MD02G1271000.v1.1

anion transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004686
Physical Location & Seq
Reverse (-)
32566873 .. 32571210
4338 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1271000.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGAGGACGATGAAATTTCCAAAGCGTTATTTAGTCGTCATATTGACCTTCATCTGCACATCTGTTTGCTATATAGAACGTGTCGGGTTTTCAATTGCATACACTTTTGCTGCTGATGCTGCTGGGGTAAATCAAGCAAGTAAAGGCACAATTCTTTCTACATTCTATTATGGCTATGCTTGTTCACAGGTGCCTGGAGGCTGGGCAGCCCAGAAAATTGGGGGAAGGAAGGTTCTTCTCCTTTCATTTGTATTATGGTCAGCTACTTGTGCATTGGTCCCTTTGGACCCCAATCGAGTCTTTGTCATGGTAATTGCTCGGCTGCTTGTTGGTGTCGCACAAGGTTTTATATTCCCCTCAATCCACACAGTTCTAGCACAGTGGGTTCCACCGCATGAAAGATCCAGATCTGTTTCTCTTACGACTTCTGGTATGTACCTAGGTGCCGCTGCAGGAATGCTTTTTCTTCCGAGCCTTGTAAAGTATAGAGGCCCGCAGGATGTCTTTCTTGCTGAGGCAGCACTAGGTGCCATGTGGTCTTTGCTTTGGTTCAAGTATGCCAGCGACCCACCTCGCTCAGAACATCCAAAAGCCACAGCTGCTGGTTTTGGGGAGTCAATGTTGCCAATCAATGGAAGTCATAAATTGAAAATAGAGAATGGAGGAAGTTCCATCAGATCTGCGAAAATCCCATGGAAGAGAATTTTACTCAGTTTGCCAGTTTGGGCAATTGTGGTTAATAATTTCACATTTCACTACGCTTTGTATGTGTTAATGAACTGGCTGCCGACATACTTTGAATTGGGCCTCCAGCTTAGCCTTCAGGAAATGGGTTCTTCTAAGATGTTGCCGTATCTCAACATGTTTATTTTCTCAAACATAGGTGGGGTGGTTGCTGATCACTTGGTCACAAAGAGAATCTTGTCTGTTACTAGAACGCGGAAGCTCTTGAACACCATAGGGTTCATAGTTGCTTCTCTTGCACTGATGGCAATTCCCATCTTCAGAACGTCTAGTGGAGCTGTCCTCTGCTCTTCTGTTGCTCTGGGTTTCTTGGCACTTGGGAGAGCTGGTTTTGCAGTGAACCACATGGATATTGCCCCGAGATATGCAGGCATTGTGATGGGAGTTTCCAACACTGCTGGTACGTTAGCCGGCATTATTGGAGTTGATCTGACCGGCAAACTTCTTGAAGCCGCAAGAAGTGTTGATTCAAGTCTTTCGAGTCCAGAGAGCTGGAGATTGGTGTTCTTTATCCCGGGTTTGCTCTGCATTTTCAGTTCTCTAGTATTTTTACTGTTCTCGACGGGGGAGAGAATTTTTGACTGA

Protein Analysis

443

Amino Acids

48.07

Weight (kDa)

9.75

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 17 - 389 3.9e-49 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 190, 443, 527
AccB7I CCANNNNNTGG 1 cut(s) 632
AccII CGCG 1 cut(s) 940
AciI CCGC 5 cut(s) 392, 447, 494, 940, 1197
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 3 cut(s) 14, 702, 1317
AcuI CTGAAG 2 cut(s) 806, 988
AdeI CACNNNGTG 1 cut(s) 527
AfaI GTAC 2 cut(s) 437, 1147
AfiI CCNNNNNNNGG 1 cut(s) 632
AflIII ACRYGT 2 cut(s) 79, 861
AgsI TTSAA 7 cut(s) 93, 553, 649, 800, 952, 1193, 1215
AjnI CCWGG 1 cut(s) 193
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 7 cut(s) 263, 599, 814, 946, 1022, 1070, 1236
AluI AGCT 7 cut(s) 263, 599, 814, 946, 1022, 1070, 1236
AlwI GGATC 1 cut(s) 396
AlwNI CAGNNNCTG 1 cut(s) 602
Ama87I CYCGRG 2 cut(s) 1102, 1258
AoxI GGCC 2 cut(s) 490, 805
ApeKI GCWGC 8 cut(s) 110, 119, 206, 322, 449, 518, 599, 784
ApoI RAATTY 3 cut(s) 14, 702, 1317
AspA2I CCTAGG 1 cut(s) 439
AspS9I GGNCC 4 cut(s) 277, 286, 491, 805
AsuC2I CCSGG 2 cut(s) 1259, 1260
AvaI CYCGRG 2 cut(s) 1102, 1258
AvaII GGWCC 2 cut(s) 277, 286
AvrII CCTAGG 1 cut(s) 439
BanI GGYRCC 3 cut(s) 190, 443, 527
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 8 cut(s) 97, 106, 218, 309, 436, 530, 586, 771
BccI CCATC 4 cut(s) 680, 982, 1007, 1117
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 2 cut(s) 1259, 1260
BfaI CTAG 6 cut(s) 374, 440, 524, 933, 1014, 1286
BfmI CTRYAG 1 cut(s) 450
BglII AGATCT 2 cut(s) 407, 677
BlnI CCTAGG 1 cut(s) 439
BlpI GCTNAGC 1 cut(s) 815
Bme1390I CCNGG 3 cut(s) 195, 1259, 1260
Bme18I GGWCC 2 cut(s) 277, 286
BmeT110I CYCGRG 2 cut(s) 1102, 1258
BmgT120I GGNCC 4 cut(s) 277, 286, 491, 805
BmiI GGNNCC 6 cut(s) 192, 279, 288, 387, 445, 529
BmrFI CCNGG 3 cut(s) 195, 1259, 1260
BmsI GCATC 1 cut(s) 106
BplI GAGNNNNNCTC 2 cut(s) 1011, 1043
BpmI CTGGAG 3 cut(s) 216, 794, 1258
Bpu10I CCTNAGC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 815
BpuMI CCSGG 2 cut(s) 1259, 1260
BsaBI GATNNNNATC 1 cut(s) 406
BsaJI CCNNGG 3 cut(s) 439, 692, 1258
BsaXI ACNNNNNCTCC 4 cut(s) 605, 635, 1057, 1087
Bsc4I CCNNNNNNNGG 1 cut(s) 632
Bse118I RCCGGY 2 cut(s) 1154, 1178
Bse1I ACTGG 2 cut(s) 719, 785
Bse8I GATNNNNATC 1 cut(s) 406
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 3 cut(s) 439, 692, 1258
BseGI GGATG 2 cut(s) 505, 583
BseJI GATNNNNATC 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 632
BseMII CTCAG 3 cut(s) 504, 591, 724
BseNI ACTGG 2 cut(s) 719, 785
BseXI GCAGC 8 cut(s) 97, 106, 218, 309, 436, 530, 586, 771
BseYI CCCAGC 2 cut(s) 122, 201
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 940
BshFI GGCC 2 cut(s) 492, 807
BshNI GGYRCC 3 cut(s) 190, 443, 527
BsiHKCI CYCGRG 2 cut(s) 1102, 1258
BsiSI CCGG 3 cut(s) 1155, 1179, 1259
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 632
BsmFI GGGAC 1 cut(s) 263
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 2 cut(s) 492, 807
BsoBI CYCGRG 2 cut(s) 1102, 1258
Bsp143I GATC 5 cut(s) 401, 407, 677, 898, 1171
Bsp1720I GCTNAGC 1 cut(s) 815
Bsp19I CCATGG 1 cut(s) 692
BspACI CCGC 5 cut(s) 392, 447, 494, 940, 1197
BspANI GGCC 2 cut(s) 492, 807
BspCNI CTCAG 3 cut(s) 505, 590, 723
BspFNI CGCG 1 cut(s) 940
BspLI GGNNCC 6 cut(s) 192, 279, 288, 387, 445, 529
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 1 cut(s) 396
BspQI GCTCTTC 1 cut(s) 1039
BspT107I GGYRCC 3 cut(s) 190, 443, 527
BsrFI RCCGGY 2 cut(s) 1154, 1178
BsrI ACTGG 2 cut(s) 719, 785
BssAI RCCGGY 2 cut(s) 1154, 1178
BssECI CCNNGG 3 cut(s) 439, 692, 1258
BssMI GATC 5 cut(s) 401, 407, 677, 898, 1171
BssT1I CCWWGG 2 cut(s) 439, 692
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 3 cut(s) 370, 381, 1299
Bst6I CTCTTC 2 cut(s) 692, 1039
BstAPI GCANNNNNTGC 1 cut(s) 527
BstC8I GCNNGC 4 cut(s) 494, 562, 1114, 1156
BstDEI CTNAG 5 cut(s) 513, 577, 710, 815, 840
BstDSI CCRYGG 1 cut(s) 692
BstF5I GGATG 2 cut(s) 505, 583
BstFNI CGCG 1 cut(s) 940
BstKTI GATC 5 cut(s) 404, 410, 680, 901, 1174
BstMBI GATC 5 cut(s) 401, 407, 677, 898, 1171
BstMWI GCNNNNNNNGC 9 cut(s) 116, 119, 269, 518, 527, 599, 980, 989, 1076
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 865
BstSCI CCNGG 3 cut(s) 193, 1257, 1258
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 1 cut(s) 940
BstV1I GCAGC 8 cut(s) 97, 106, 218, 309, 436, 530, 586, 771
BstX2I RGATCY 3 cut(s) 401, 407, 677
BstXI CCANNNNNNTGG 2 cut(s) 218, 1236
BstYI RGATCY 3 cut(s) 401, 407, 677
BsuRI GGCC 2 cut(s) 492, 807
BtgI CCRYGG 1 cut(s) 692
BtsCI GGATG 2 cut(s) 505, 583
BtsI GCAGTG 2 cut(s) 1086, 1137
BtsIMutI CAGTG 4 cut(s) 386, 983, 1086, 1137
Cac8I GCNNGC 4 cut(s) 494, 562, 1114, 1156
CaiI CAGNNNCTG 1 cut(s) 602
Cfr10I RCCGGY 2 cut(s) 1154, 1178
Cfr13I GGNCC 4 cut(s) 277, 286, 491, 805
Cfr9I CCCGGG 1 cut(s) 1258
Csp6I GTAC 2 cut(s) 436, 1146
CviAII CATG 6 cut(s) 307, 395, 532, 693, 862, 1090
CviQI GTAC 2 cut(s) 436, 1146
DdeI CTNAG 5 cut(s) 513, 577, 710, 815, 840
DpnI GATC 5 cut(s) 403, 409, 679, 900, 1173
DpnII GATC 5 cut(s) 401, 407, 677, 898, 1171
DraIII CACNNNGTG 1 cut(s) 527
Eam1104I CTCTTC 2 cut(s) 692, 1039
EarI CTCTTC 2 cut(s) 692, 1039
Eco130I CCWWGG 2 cut(s) 439, 692
Eco47I GGWCC 2 cut(s) 277, 286
Eco57I CTGAAG 2 cut(s) 806, 988
Eco88I CYCGRG 2 cut(s) 1102, 1258
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 439, 692
ErhI CCWWGG 2 cut(s) 439, 692
FaeI CATG 6 cut(s) 310, 398, 535, 696, 865, 1093
FalI AAGNNNNNCTT 2 cut(s) 905, 937
FaqI GGGAC 1 cut(s) 263
FatI CATG 6 cut(s) 306, 394, 531, 692, 861, 1089
FauI CCCGC 1 cut(s) 501
FbaI TGATCA 1 cut(s) 898
FokI GGATG 2 cut(s) 512, 570
FspBI CTAG 6 cut(s) 374, 440, 524, 933, 1014, 1286
GsaI CCCAGC 2 cut(s) 126, 205
GsuI CTGGAG 3 cut(s) 216, 794, 1258
HaeIII GGCC 2 cut(s) 492, 807
HapII CCGG 3 cut(s) 1155, 1179, 1259
Hin1II CATG 6 cut(s) 310, 398, 535, 696, 865, 1093
HinfI GANTC 5 cut(s) 297, 614, 918, 1211, 1225
HpaII CCGG 3 cut(s) 1155, 1179, 1259
Hpy166II GTNNAC 2 cut(s) 185, 1084
Hpy188I TCNGA 5 cut(s) 471, 580, 677, 1007, 1176
Hpy188III TCNNGA 6 cut(s) 405, 824, 949, 1190, 1229, 1303
Hpy8I GTNNAC 2 cut(s) 185, 1084
Hpy99I CGWCG 1 cut(s) 1309
HpyAV CCTTC 4 cut(s) 58, 219, 223, 830
HpyCH4III ACNGT 3 cut(s) 370, 381, 1299
HpyCH4IV ACGT 3 cut(s) 79, 1010, 1148
HpyCH4V TGCA 8 cut(s) 57, 98, 272, 452, 983, 1079, 1112, 1272
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 119, 269, 518, 527, 599, 980, 989, 1076
HpyF3I CTNAG 5 cut(s) 513, 577, 710, 815, 840
HpySE526I ACGT 3 cut(s) 79, 1010, 1148
Hsp92II CATG 6 cut(s) 310, 398, 535, 696, 865, 1093
KroI GCCGGC 1 cut(s) 1154
KroNI GCCGGC 1 cut(s) 1156
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 5 cut(s) 401, 407, 677, 898, 1171
LguI GCTCTTC 1 cut(s) 1039
LmnI GCTCC 1 cut(s) 1019
Lsp1109I GCAGC 8 cut(s) 97, 106, 218, 309, 436, 530, 586, 771
LweI GCATC 1 cut(s) 106
MaeI CTAG 6 cut(s) 374, 440, 524, 933, 1014, 1286
MaeII ACGT 3 cut(s) 79, 1010, 1148
MaeIII GTNAC 2 cut(s) 907, 928
MalI GATC 5 cut(s) 403, 409, 679, 900, 1173
MboI GATC 5 cut(s) 401, 407, 677, 898, 1171
MboII GAAGA 6 cut(s) 227, 458, 709, 828, 994, 1026
MfeI CAATTG 2 cut(s) 93, 729
MflI RGATCY 3 cut(s) 401, 407, 677
MlyI GAGTC 3 cut(s) 306, 623, 1234
MmeI TCCRAC 1 cut(s) 1158
MnlI CCTC 8 cut(s) 191, 367, 482, 508, 582, 656, 818, 1037
MroNI GCCGGC 1 cut(s) 1154
MseI TTAA 2 cut(s) 738, 773
MslI CAYNNNNRTG 1 cut(s) 1121
MspA1I CMGCKG 2 cut(s) 449, 599
MspI CCGG 3 cut(s) 1155, 1179, 1259
MspR9I CCNGG 3 cut(s) 195, 1259, 1260
MunI CAATTG 2 cut(s) 93, 729
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 1 cut(s) 940
MwoI GCNNNNNNNGC 9 cut(s) 116, 119, 269, 518, 527, 599, 980, 989, 1076
NaeI GCCGGC 1 cut(s) 1156
NciI CCSGG 2 cut(s) 1259, 1260
NcoI CCATGG 1 cut(s) 692
NdeII GATC 5 cut(s) 401, 407, 677, 898, 1171
NgoMIV GCCGGC 1 cut(s) 1154
NlaIII CATG 6 cut(s) 310, 398, 535, 696, 865, 1093
NlaIV GGNNCC 6 cut(s) 192, 279, 288, 387, 445, 529
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 907
NspI RCATGY 1 cut(s) 865
PciI ACATGT 1 cut(s) 861
PciSI GCTCTTC 1 cut(s) 1039
PctI GAATGC 1 cut(s) 462
PdiI GCCGGC 1 cut(s) 1156
PfeI GAWTC 2 cut(s) 918, 1211
PflMI CCANNNNNTGG 1 cut(s) 632
PleI GAGTC 3 cut(s) 305, 622, 1233
PpsI GAGTC 3 cut(s) 305, 622, 1233
PscI ACATGT 1 cut(s) 861
Psp6I CCWGG 1 cut(s) 193
PspFI CCCAGC 2 cut(s) 122, 201
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 6 cut(s) 192, 279, 288, 387, 445, 529
PspPI GGNCC 4 cut(s) 277, 286, 491, 805
PstI CTGCAG 1 cut(s) 454
PstNI CAGNNNCTG 1 cut(s) 602
PsuI RGATCY 3 cut(s) 401, 407, 677
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 2 cut(s) 437, 1147
RsaNI GTAC 2 cut(s) 436, 1146
RseI CAYNNNNRTG 1 cut(s) 1121
SapI GCTCTTC 1 cut(s) 1039
SaqAI TTAA 2 cut(s) 738, 773
Sau3AI GATC 5 cut(s) 401, 407, 677, 898, 1171
Sau96I GGNCC 4 cut(s) 277, 286, 491, 805
SchI GAGTC 3 cut(s) 306, 623, 1234
ScrFI CCNGG 3 cut(s) 195, 1259, 1260
SfaNI GCATC 1 cut(s) 106
SfcI CTRYAG 1 cut(s) 450
SinI GGWCC 2 cut(s) 277, 286
SmaI CCCGGG 1 cut(s) 1260
SmiMI CAYNNNNRTG 1 cut(s) 1121
SsiI CCGC 5 cut(s) 392, 447, 494, 940, 1197
SspMI CTAG 6 cut(s) 374, 440, 524, 933, 1014, 1286
StyD4I CCNGG 3 cut(s) 193, 1257, 1258
StyI CCWWGG 2 cut(s) 439, 692
TaaI ACNGT 3 cut(s) 370, 381, 1299
TaiI ACGT 3 cut(s) 82, 1013, 1151
TaqI TCGA 3 cut(s) 295, 1223, 1304
TauI GCSGC 2 cut(s) 449, 1199
TfiI GAWTC 2 cut(s) 918, 1211
Tru1I TTAA 2 cut(s) 738, 773
Tru9I TTAA 2 cut(s) 738, 773
TscAI CASTG 4 cut(s) 386, 990, 1086, 1144
TseFI GTSAC 1 cut(s) 907
TseI GCWGC 8 cut(s) 110, 119, 206, 322, 449, 518, 599, 784
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 6 cut(s) 26, 40, 234, 411, 791, 955
TspMI CCCGGG 1 cut(s) 1258
TspRI CASTG 4 cut(s) 386, 990, 1086, 1144
Van91I CCANNNNNTGG 1 cut(s) 632
VpaK11BI GGWCC 2 cut(s) 277, 286
XapI RAATTY 3 cut(s) 14, 702, 1317
XceI RCATGY 1 cut(s) 865
XmaI CCCGGG 1 cut(s) 1258
XmaJI CCTAGG 1 cut(s) 439
XspI CTAG 6 cut(s) 374, 440, 524, 933, 1014, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.