Rroxscaffold_7G00187490

anion transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004686
Physical Location & Seq
Reverse (-)
26898670 .. 26899767
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187490.1

Sequence Viewer

Length: 1098 bp
ATGCCAGTTCACAAATTCCTGGAGGTTGGGCAGCTCAAAAGTTTGGAGGAAGGTCTGTGCTCCTCATCTCATTTGTTTCATGGTCCCTTAACATATGCTTTTGTCCCTCTGGACCCTAAAAATGTCAAAACTATGGTTTTGGCTCGGTTGCTTGTTGGTGTGGCACAGGGGTTCATGTTCCCTTCCATCCACTCTGTTCTCGCTCAATGGGTTCCACAAAATGAGAGGTCCAGATCAGTTTCCCTCACAACTTCTGGGATGTACCTTGGTGCAGCTGCAGGTATGCTTGTGGTTCCAACCCTAGTGAAGTTGACAAGCCCCCAATTTGTGTTTTTGGGTGAAGCAACTTTAGGGACTATTTGGTCAGTGCTTTGGTTAAACTATGCTAGTGAACCACCAATTCAATTGTCTAAGCAAAATGGAGCATTTCCTAATTCTAATAGAAAAACCAAAATTCCATGGAAGAGTATTTTTAGAAGCTTACCTATTTGGGCTATTGTGGTGAACAATTTCACTTTCCACTATGCTTTATATGTCATGATGAATTGGCTTCCAACATACTTTGAACAAGGCCTGAAGCTTAGTCTCCAAGAAATGGGGACTTACAAAATGGTGCCTTATTTCAACATGTTCATGTTCTCAAACATTGGTGGATTTGTAGCTGATTATTTAATCACAAAGAGGCTCATGTCTGTGACTAGAACTAGAAAGTTCTTGAACACTTTAGGGTTCATAGTTGCTTCTATTGCATTGATGGCACTGCCCAAATTCAGAACTTCAATTGGGGCCGTGATTTGTTCTTCATTTGCACTTGGGTTTTTGGCTTTAGGCAGAGCTGGGTTTGCAGTGAATCACATGGACATTGCTCCAAGGTATGCAGGAATTGTGATGGGTGTGTCTAACACAGCTGGTACATTAGCTGGGATAATTGGGGTTGATCTCACTGGGAAACTTTTAGAAGCTTCTAGATCTGCTCATGATTCAGATCTTTCGAGTCCAGAAAGCTGGACATCAGTTTTCATGATTCCTGGGGTGCTTTGCATTTTCAGCTCAATCGGGTTTTTGATCTTCTCAACTGGGGAGAGGATTTTTGACTGA

Protein Analysis

365

Amino Acids

40.15

Weight (kDa)

9.56

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 40 - 311 1.1e-35 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 361
Acc36I ACCTGC 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 595
AcsI RAATTY 3 cut(s) 14, 453, 767
AcuI CTGAAG 1 cut(s) 596
AfaI GTAC 2 cut(s) 263, 913
AfiI CCNNNNNNNGG 1 cut(s) 595
AflIII ACRYGT 1 cut(s) 627
AgsI TTSAA 5 cut(s) 404, 566, 625, 718, 780
AjnI CCWGG 2 cut(s) 18, 1027
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 590
AoxI GGCC 2 cut(s) 571, 786
ApeKI GCWGC 3 cut(s) 31, 272, 275
ApoI RAATTY 3 cut(s) 14, 453, 767
Asp700I GAANNNNTTC 1 cut(s) 509
AspS9I GGNCC 4 cut(s) 83, 112, 228, 786
AsuHPI GGTGA 2 cut(s) 350, 514
AvaII GGWCC 3 cut(s) 83, 112, 228
BanI GGYRCC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 3 cut(s) 43, 262, 284
BccI CCATC 3 cut(s) 194, 748, 883
BceAI ACGGC 1 cut(s) 773
BciT130I CCWGG 2 cut(s) 20, 1029
BcoDI GTCTC 1 cut(s) 590
BfaI CTAG 5 cut(s) 302, 387, 699, 705, 966
BfmI CTRYAG 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 269
BglII AGATCT 2 cut(s) 968, 985
BisI GCNGC 3 cut(s) 32, 273, 276
BlsI GCNGC 3 cut(s) 33, 274, 277
Bme1390I CCNGG 2 cut(s) 20, 1029
Bme18I GGWCC 3 cut(s) 83, 112, 228
BmgT120I GGNCC 4 cut(s) 83, 112, 228, 786
BmiI GGNNCC 6 cut(s) 85, 114, 213, 294, 615, 787
BmrFI CCNGG 2 cut(s) 20, 1029
BmrI ACTGGG 2 cut(s) 954, 1086
BmuI ACTGGG 2 cut(s) 954, 1086
BpmI CTGGAG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 984
BsaJI CCNNGG 4 cut(s) 265, 458, 869, 1028
Bsc4I CCNNNNNNNGG 1 cut(s) 595
Bse1I ACTGG 3 cut(s) 5, 949, 1081
Bse3DI GCAATG 1 cut(s) 861
Bse8I GATNNNNATC 1 cut(s) 984
BseBI CCWGG 2 cut(s) 20, 1029
BseDI CCNNGG 4 cut(s) 265, 458, 869, 1028
BseGI GGATG 2 cut(s) 186, 264
BseJI GATNNNNATC 1 cut(s) 984
BseLI CCNNNNNNNGG 1 cut(s) 595
BseMI GCAATG 1 cut(s) 861
BseNI ACTGG 3 cut(s) 5, 949, 1081
BseRI GAGGAG 1 cut(s) 52
BseXI GCAGC 3 cut(s) 43, 262, 284
BseYI CCCAGC 2 cut(s) 836, 920
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 2 cut(s) 573, 788
BshNI GGYRCC 1 cut(s) 613
BsiHKAI GWGCWC 1 cut(s) 62
BslFI GGGAC 4 cut(s) 69, 89, 367, 613
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 1 cut(s) 590
BsmFI GGGAC 4 cut(s) 69, 89, 367, 613
BsnI GGCC 2 cut(s) 573, 788
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 5 cut(s) 233, 937, 968, 985, 1065
Bsp19I CCATGG 1 cut(s) 458
BspANI GGCC 2 cut(s) 573, 788
BspHI TCATGA 3 cut(s) 537, 976, 1020
BspLI GGNNCC 6 cut(s) 85, 114, 213, 294, 615, 787
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 269
BspT107I GGYRCC 1 cut(s) 613
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 3 cut(s) 5, 949, 1081
BssECI CCNNGG 4 cut(s) 265, 458, 869, 1028
BssMI GATC 5 cut(s) 233, 937, 968, 985, 1065
BssT1I CCWWGG 3 cut(s) 265, 458, 869
Bst2UI CCWGG 2 cut(s) 20, 1029
Bst6I CTCTTC 1 cut(s) 458
BstDEI CTNAG 2 cut(s) 411, 581
BstDSI CCRYGG 1 cut(s) 458
BstF5I GGATG 2 cut(s) 186, 264
BstKTI GATC 5 cut(s) 236, 940, 971, 988, 1068
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 5 cut(s) 233, 937, 968, 985, 1065
BstMWI GCNNNNNNNGC 4 cut(s) 746, 755, 842, 1047
BstNI CCWGG 2 cut(s) 20, 1029
BstNSI RCATGY 1 cut(s) 631
BstSCI CCNGG 2 cut(s) 18, 1027
BstSFI CTRYAG 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 43, 262, 284
BstX2I RGATCY 2 cut(s) 968, 985
BstXI CCANNNNNNTGG 1 cut(s) 1005
BstYI RGATCY 2 cut(s) 968, 985
BsuRI GGCC 2 cut(s) 573, 788
BtgI CCRYGG 1 cut(s) 458
BtsCI GGATG 2 cut(s) 186, 264
BtsI GCAGTG 2 cut(s) 758, 852
BtsIMutI CAGTG 4 cut(s) 372, 758, 852, 942
BveI ACCTGC 1 cut(s) 269
CciI TCATGA 3 cut(s) 537, 976, 1020
Cfr13I GGNCC 4 cut(s) 83, 112, 228, 786
Csp6I GTAC 2 cut(s) 262, 912
CviQI GTAC 2 cut(s) 262, 912
DdeI CTNAG 2 cut(s) 411, 581
DpnI GATC 5 cut(s) 235, 939, 970, 987, 1067
DpnII GATC 5 cut(s) 233, 937, 968, 985, 1065
DrdI GACNNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
Eco130I CCWWGG 3 cut(s) 265, 458, 869
Eco147I AGGCCT 1 cut(s) 573
Eco47I GGWCC 3 cut(s) 83, 112, 228
Eco57I CTGAAG 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 18, 1027
EcoT14I CCWWGG 3 cut(s) 265, 458, 869
ErhI CCWWGG 3 cut(s) 265, 458, 869
FaqI GGGAC 4 cut(s) 69, 89, 367, 613
FauNDI CATATG 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 32, 273, 276
FokI GGATG 2 cut(s) 173, 271
Fsp4HI GCNGC 3 cut(s) 32, 273, 276
FspBI CTAG 5 cut(s) 302, 387, 699, 705, 966
GluI GCNGC 3 cut(s) 32, 273, 276
GsaI CCCAGC 2 cut(s) 840, 924
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 2 cut(s) 573, 788
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 3 cut(s) 478, 578, 960
HinfI GANTC 4 cut(s) 850, 980, 994, 1024
HphI GGTGA 2 cut(s) 350, 514
Hpy166II GTNNAC 4 cut(s) 10, 312, 392, 505
Hpy188I TCNGA 2 cut(s) 773, 985
Hpy188III TCNNGA 8 cut(s) 110, 231, 538, 715, 966, 977, 998, 1021
Hpy8I GTNNAC 4 cut(s) 10, 312, 392, 505
HpyAV CCTTC 2 cut(s) 44, 192
HpyCH4V TGCA 7 cut(s) 272, 278, 749, 809, 845, 878, 1041
HpyF10VI GCNNNNNNNGC 4 cut(s) 746, 755, 842, 1047
HpyF3I CTNAG 2 cut(s) 411, 581
Kzo9I GATC 5 cut(s) 233, 937, 968, 985, 1065
LmnI GCTCC 3 cut(s) 65, 422, 871
Lsp1109I GCAGC 3 cut(s) 43, 262, 284
MaeI CTAG 5 cut(s) 302, 387, 699, 705, 966
MaeIII GTNAC 1 cut(s) 694
MalI GATC 5 cut(s) 235, 939, 970, 987, 1067
MboI GATC 5 cut(s) 233, 937, 968, 985, 1065
MboII GAAGA 3 cut(s) 475, 792, 1060
MfeI CAATTG 2 cut(s) 404, 780
MflI RGATCY 2 cut(s) 968, 985
MhlI GDGCHC 1 cut(s) 62
MlyI GAGTC 1 cut(s) 1003
MmeI TCCRAC 2 cut(s) 320, 578
MnlI CCTC 8 cut(s) 16, 40, 73, 117, 219, 254, 675, 1077
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 3 cut(s) 89, 377, 671
MslI CAYNNNNRTG 2 cut(s) 632, 692
MspA1I CMGCKG 2 cut(s) 275, 908
MspR9I CCNGG 2 cut(s) 20, 1029
MunI CAATTG 2 cut(s) 404, 780
MvaI CCWGG 2 cut(s) 20, 1029
MwoI GCNNNNNNNGC 4 cut(s) 746, 755, 842, 1047
NcoI CCATGG 1 cut(s) 458
NdeI CATATG 1 cut(s) 94
NdeII GATC 5 cut(s) 233, 937, 968, 985, 1065
NlaIV GGNNCC 6 cut(s) 85, 114, 213, 294, 615, 787
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 1 cut(s) 631
PagI TCATGA 3 cut(s) 537, 976, 1020
PceI AGGCCT 1 cut(s) 573
PciI ACATGT 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 3 cut(s) 850, 980, 1024
PflMI CCANNNNNTGG 1 cut(s) 595
PfoI TCCNGGA 1 cut(s) 18
PkrI GCNGC 3 cut(s) 33, 274, 277
PleI GAGTC 1 cut(s) 1002
PpsI GAGTC 1 cut(s) 1002
PscI ACATGT 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 18, 1027
PspFI CCCAGC 2 cut(s) 836, 920
PspGI CCWGG 2 cut(s) 18, 1027
PspN4I GGNNCC 6 cut(s) 85, 114, 213, 294, 615, 787
PspPI GGNCC 4 cut(s) 83, 112, 228, 786
PstI CTGCAG 1 cut(s) 280
PsuI RGATCY 2 cut(s) 968, 985
PvuII CAGCTG 2 cut(s) 275, 908
RsaI GTAC 2 cut(s) 263, 913
RsaNI GTAC 2 cut(s) 262, 912
RseI CAYNNNNRTG 2 cut(s) 632, 692
SaqAI TTAA 3 cut(s) 89, 377, 671
SatI GCNGC 3 cut(s) 32, 273, 276
Sau3AI GATC 5 cut(s) 233, 937, 968, 985, 1065
Sau96I GGNCC 4 cut(s) 83, 112, 228, 786
SchI GAGTC 1 cut(s) 1003
ScrFI CCNGG 2 cut(s) 20, 1029
SduI GDGCHC 1 cut(s) 62
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 3 cut(s) 83, 112, 228
SmiMI CAYNNNNRTG 2 cut(s) 632, 692
SseBI AGGCCT 1 cut(s) 573
SspMI CTAG 5 cut(s) 302, 387, 699, 705, 966
StuI AGGCCT 1 cut(s) 573
StyD4I CCNGG 2 cut(s) 18, 1027
StyI CCWWGG 3 cut(s) 265, 458, 869
TaqI TCGA 1 cut(s) 992
TfiI GAWTC 3 cut(s) 850, 980, 1024
Tru1I TTAA 3 cut(s) 89, 377, 671
Tru9I TTAA 3 cut(s) 89, 377, 671
TscAI CASTG 4 cut(s) 372, 765, 852, 949
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 3 cut(s) 31, 272, 275
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 7 cut(s) 68, 163, 557, 622, 721, 792, 1009
TspRI CASTG 4 cut(s) 372, 765, 852, 949
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 3 cut(s) 83, 112, 228
XapI RAATTY 3 cut(s) 14, 453, 767
XbaI TCTAGA 1 cut(s) 965
XceI RCATGY 1 cut(s) 631
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 5 cut(s) 302, 387, 699, 705, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.