FvH4_3g38550

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003783
Physical Location & Seq
Forward (+)
32895552 .. 32898516
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38550.t1

Sequence Viewer

Length: 1161 bp
ATGGGGAGAAACCGAACCACCCACCTCACTTCCTCCCCTCGCCGCTGCCTGATTGCCGGACACTACTGCCATGACGTTCTCATCCGCGACGGCGTCGTTTTGGCCGAGACCCTCGGCGGCGCAGCTTCGTTTATCTCCCACGTCTTCGATGGCGTGTCCGTCACTTACAATCTGGTGTCCAAAGTCGGGCCGGATTTCGCTTACTCGGTCCACCGCGACCCGATTGTGGTGCCCGATTCCCGGACAACTCTGTTCCACGCGCATTTTGATTCCGGAATCCACGGGGAGGGGCAGCAGGACCGGGTCCTGAAGCGGGTAAATGCCTGCGACCCGATTCGGCCCTCGGATCTGCCCGATTCCAGGTTTGATTTCGGAATGGTGGTTGGGGTTGGTGGCGAGATTACGGTGGAGACGGTTGAGAAGCTGGTGGAGATTTGTGGCACTGTGTTTGTGGACATTCAGGCTCTGATTCGGGTTTTTGATGAGGTTGATGGGTCTGTGAGGCTTGTGGGTTTGAAAGAGAGTGGGTTCTGTCACCTCTTGCCGAGAATTGGGTTTATAAAGGCGTCGGCGGAGGAGGCTCTGTATATGGACGTGGAGGAGGTCAGGAAGGTGTGCTGTGTTGTGGTGACGAATGGGAAACGTGGGTGTAGAGTGTGTTGGAGAGATGGGGAAGTGGAAGTTGGGCCGTTTCCGACGACCCAGGTGGATCCAACTGGTGCTGGAGATAGTTTTCTTGGTGGGTTTGTTGCGGGGCTTGTTAATGGGTTGGCTGTGCCTGATGCTGCATTGCTTGGGAACTTGTTTGGGTCACTGACTGTGGGGCAAATCGGACTGCCCAAGTTCGACTTGAGGATGTTACAGAGAGTTCAGGAAGAGGTGCAGAAGAGAAAGGTACAGTGTCTCAGCTGGCACGAGAGAATAGACGAGAAGTTCAGGTTTATGAATGTAGCAGGACATGAACAATTTCATGAATCCCTCATAGCAGCTAAACAGATACCAAGGTGCCTTCCTCTTGAATGTGAATGGGATCTGCCAAGTTCTCCTTCCAGACCAGTGGAGCAGGGTTTGGGCGTCCTCCCCCATTTCGCAACACAGCCAAAACTTCTACATGATTCCGTCTATGAGGAGCCAATTCGAACAGTGGACCTTAAACCATGA

Protein Analysis

387

Amino Acids

42.48

Weight (kDa)

5.79

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 207 - 278 3.7e-19 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB1I GGYRCC 2 cut(s) 229, 1005
AccII CGCG 3 cut(s) 87, 216, 260
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 7 cut(s) 43, 85, 117, 214, 313, 572, 752
AclWI GGATC 4 cut(s) 354, 704, 717, 1038
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 3 cut(s) 93, 566, 1074
AfaI GTAC 1 cut(s) 897
AfiI CCNNNNNNNGG 7 cut(s) 186, 226, 240, 286, 313, 360, 551
AgsI TTSAA 2 cut(s) 517, 1019
AjiI CACGTC 2 cut(s) 142, 595
AjnI CCWGG 2 cut(s) 359, 702
AjuI GAANNNNNNNTTGG 4 cut(s) 666, 698, 1030, 1062
AluBI AGCT 4 cut(s) 125, 424, 909, 989
AluI AGCT 4 cut(s) 125, 424, 909, 989
Alw26I GTCTC 3 cut(s) 101, 404, 908
AlwI GGATC 4 cut(s) 354, 704, 717, 1038
AlwNI CAGNNNCTG 1 cut(s) 466
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 4 cut(s) 102, 188, 338, 686
ApeKI GCWGC 5 cut(s) 45, 122, 292, 785, 986
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 2 cut(s) 122, 262
AspS9I GGNCC 7 cut(s) 188, 208, 298, 304, 339, 686, 1147
AsuC2I CCSGG 2 cut(s) 241, 302
AsuHPI GGTGA 2 cut(s) 527, 640
AsuII TTCGAA 1 cut(s) 1138
AvaII GGWCC 4 cut(s) 208, 298, 304, 1147
BaeGI GKGCMC 1 cut(s) 234
BaeI ACNNNNGTAYC 2 cut(s) 989, 1022
BamHI GGATCC 1 cut(s) 709
BanI GGYRCC 2 cut(s) 229, 1005
BauI CACGAG 1 cut(s) 914
BbsI GAAGAC 1 cut(s) 136
BbvI GCAGC 5 cut(s) 32, 134, 304, 772, 998
BccI CCATC 3 cut(s) 143, 485, 662
BceAI ACGGC 2 cut(s) 106, 673
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 2 cut(s) 361, 704
BcnI CCSGG 2 cut(s) 241, 302
BcoDI GTCTC 3 cut(s) 101, 404, 908
BisI GCNGC 7 cut(s) 43, 46, 118, 123, 293, 786, 987
BlsI GCNGC 7 cut(s) 44, 47, 119, 124, 294, 787, 988
Bme1390I CCNGG 4 cut(s) 241, 302, 361, 704
Bme18I GGWCC 4 cut(s) 208, 298, 304, 1147
BmgBI CACGTC 2 cut(s) 142, 595
BmgT120I GGNCC 7 cut(s) 188, 208, 298, 304, 339, 686, 1147
BmiI GGNNCC 5 cut(s) 231, 305, 711, 1007, 1131
BmrFI CCNGG 4 cut(s) 241, 302, 361, 704
BmsI GCATC 1 cut(s) 772
BpiI GAAGAC 1 cut(s) 136
BpmI CTGGAG 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 1138
BpuEI CTTGAG 1 cut(s) 871
BpuMI CCSGG 2 cut(s) 241, 302
BsaHI GRCGYC 3 cut(s) 93, 566, 1074
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 5 cut(s) 112, 280, 342, 702, 1001
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 569, 599
Bsc4I CCNNNNNNNGG 7 cut(s) 186, 226, 240, 286, 313, 360, 551
Bse1I ACTGG 2 cut(s) 721, 1055
Bse3DI GCAATG 1 cut(s) 788
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 2 cut(s) 361, 704
BseDI CCNNGG 5 cut(s) 112, 280, 342, 702, 1001
BseGI GGATG 2 cut(s) 81, 861
BseLI CCNNNNNNNGG 7 cut(s) 186, 226, 240, 286, 313, 360, 551
BseMI GCAATG 1 cut(s) 788
BseMII CTCAG 1 cut(s) 919
BseNI ACTGG 2 cut(s) 721, 1055
BseRI GAGGAG 3 cut(s) 590, 614, 1142
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 5 cut(s) 32, 134, 304, 772, 998
BsgI GTGCAG 1 cut(s) 902
Bsh1236I CGCG 3 cut(s) 87, 216, 260
BshFI GGCC 4 cut(s) 104, 190, 340, 688
BshNI GGYRCC 2 cut(s) 229, 1005
BsiSI CCGG 5 cut(s) 57, 191, 241, 273, 301
BslI CCNNNNNNNGG 7 cut(s) 186, 226, 240, 286, 313, 360, 551
BsmAI GTCTC 3 cut(s) 101, 404, 908
BsmBI CGTCTC 1 cut(s) 404
BsnI GGCC 4 cut(s) 104, 190, 340, 688
Bso31I GGTCTC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 1138
Bsp1286I GDGCHC 1 cut(s) 234
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 3 cut(s) 346, 709, 1030
BspACI CCGC 7 cut(s) 43, 85, 117, 214, 313, 572, 752
BspANI GGCC 4 cut(s) 104, 190, 340, 688
BspCNI CTCAG 1 cut(s) 918
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 3 cut(s) 87, 216, 260
BspHI TCATGA 1 cut(s) 970
BspLI GGNNCC 5 cut(s) 231, 305, 711, 1007, 1131
BspPI GGATC 4 cut(s) 354, 704, 717, 1038
BspT104I TTCGAA 1 cut(s) 1138
BspT107I GGYRCC 2 cut(s) 229, 1005
BspTNI GGTCTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 788
BsrI ACTGG 2 cut(s) 721, 1055
BssECI CCNNGG 5 cut(s) 112, 280, 342, 702, 1001
BssMI GATC 3 cut(s) 346, 709, 1030
BssNI GRCGYC 3 cut(s) 93, 566, 1074
BssSI CACGAG 1 cut(s) 914
BssT1I CCWWGG 1 cut(s) 1001
Bst2BI CACGAG 1 cut(s) 914
Bst2UI CCWGG 2 cut(s) 361, 704
Bst4CI ACNGT 6 cut(s) 406, 415, 445, 820, 900, 1144
Bst6I CTCTTC 2 cut(s) 870, 881
BstACI GRCGYC 3 cut(s) 93, 566, 1074
BstBI TTCGAA 1 cut(s) 1138
BstC8I GCNNGC 2 cut(s) 325, 911
BstDEI CTNAG 1 cut(s) 905
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 81, 861
BstFNI CGCG 3 cut(s) 87, 216, 260
BstHHI GCGC 2 cut(s) 122, 262
BstKTI GATC 3 cut(s) 349, 712, 1033
BstMAI GTCTC 3 cut(s) 101, 404, 908
BstMBI GATC 3 cut(s) 346, 709, 1030
BstMWI GCNNNNNNNGC 1 cut(s) 578
BstNI CCWGG 2 cut(s) 361, 704
BstSCI CCNGG 4 cut(s) 239, 300, 359, 702
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 3 cut(s) 87, 216, 260
BstV1I GCAGC 5 cut(s) 32, 134, 304, 772, 998
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 3 cut(s) 346, 709, 1030
BstXI CCANNNNNNTGG 1 cut(s) 1057
BstYI RGATCY 3 cut(s) 346, 709, 1030
BsuRI GGCC 4 cut(s) 104, 190, 340, 688
BtgI CCRYGG 1 cut(s) 280
BtrI CACGTC 2 cut(s) 142, 595
BtsCI GGATG 2 cut(s) 81, 861
BtsIMutI CAGTG 5 cut(s) 441, 812, 905, 1062, 1149
Cac8I GCNNGC 2 cut(s) 325, 911
CaiI CAGNNNCTG 1 cut(s) 466
CciI TCATGA 1 cut(s) 970
CfoI GCGC 2 cut(s) 122, 262
Cfr13I GGNCC 7 cut(s) 188, 208, 298, 304, 339, 686, 1147
CseI GACGC 3 cut(s) 82, 555, 1063
Csp6I GTAC 1 cut(s) 896
CviAII CATG 5 cut(s) 71, 959, 971, 1112, 1158
CviQI GTAC 1 cut(s) 896
DdeI CTNAG 1 cut(s) 905
DpnI GATC 3 cut(s) 348, 711, 1032
DpnII GATC 3 cut(s) 346, 709, 1030
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 2 cut(s) 870, 881
EarI CTCTTC 2 cut(s) 870, 881
EciI GGCGGA 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 1001
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 4 cut(s) 208, 298, 304, 1147
Eco57I CTGAAG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 359, 702
EcoT14I CCWWGG 1 cut(s) 1001
ErhI CCWWGG 1 cut(s) 1001
Esp3I CGTCTC 1 cut(s) 404
FaeI CATG 5 cut(s) 74, 962, 974, 1115, 1161
FalI AAGNNNNNCTT 4 cut(s) 833, 865, 1030, 1062
FatI CATG 5 cut(s) 70, 958, 970, 1111, 1157
FauI CCCGC 2 cut(s) 306, 745
Fnu4HI GCNGC 7 cut(s) 43, 46, 118, 123, 293, 786, 987
FokI GGATG 2 cut(s) 68, 868
Fsp4HI GCNGC 7 cut(s) 43, 46, 118, 123, 293, 786, 987
GlaI GCGC 2 cut(s) 121, 261
GluI GCNGC 7 cut(s) 43, 46, 118, 123, 293, 786, 987
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 4 cut(s) 104, 190, 340, 688
HapII CCGG 5 cut(s) 57, 191, 241, 273, 301
HgaI GACGC 3 cut(s) 82, 555, 1063
HhaI GCGC 2 cut(s) 122, 262
Hin1I GRCGYC 3 cut(s) 93, 566, 1074
Hin1II CATG 5 cut(s) 74, 962, 974, 1115, 1161
Hin6I GCGC 2 cut(s) 120, 260
HinP1I GCGC 2 cut(s) 120, 260
HinfI GANTC 8 cut(s) 236, 269, 276, 334, 356, 469, 974, 1115
HpaII CCGG 5 cut(s) 57, 191, 241, 273, 301
HphI GGTGA 2 cut(s) 527, 640
Hpy166II GTNNAC 3 cut(s) 211, 454, 1147
Hpy188I TCNGA 5 cut(s) 346, 374, 468, 696, 833
Hpy188III TCNNGA 7 cut(s) 273, 307, 607, 872, 971, 1016, 1050
Hpy8I GTNNAC 3 cut(s) 211, 454, 1147
Hpy99I CGWCG 4 cut(s) 92, 98, 571, 700
HpyAV CCTTC 3 cut(s) 604, 1019, 1056
HpyCH4III ACNGT 6 cut(s) 406, 415, 445, 820, 900, 1144
HpyCH4IV ACGT 4 cut(s) 75, 141, 594, 643
HpyCH4V TGCA 2 cut(s) 788, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 578
HpyF3I CTNAG 1 cut(s) 905
HpySE526I ACGT 4 cut(s) 75, 141, 594, 643
Hsp92I GRCGYC 3 cut(s) 93, 566, 1074
Hsp92II CATG 5 cut(s) 74, 962, 974, 1115, 1161
HspAI GCGC 2 cut(s) 120, 260
Kpn2I TCCGGA 1 cut(s) 272
Kzo9I GATC 3 cut(s) 346, 709, 1030
LmnI GCTCC 2 cut(s) 1060, 1129
Lsp1109I GCAGC 5 cut(s) 32, 134, 304, 772, 998
LweI GCATC 1 cut(s) 772
MaeII ACGT 4 cut(s) 75, 141, 594, 643
MaeIII GTNAC 5 cut(s) 160, 533, 628, 810, 858
MalI GATC 3 cut(s) 348, 711, 1032
MboI GATC 3 cut(s) 346, 709, 1030
MboII GAAGA 3 cut(s) 136, 887, 898
MflI RGATCY 3 cut(s) 346, 709, 1030
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 3 cut(s) 549, 965, 1134
MmeI TCCRAC 3 cut(s) 641, 719, 737
MroI TCCGGA 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 966
MseI TTAA 2 cut(s) 762, 1152
MspA1I CMGCKG 2 cut(s) 45, 909
MspI CCGG 5 cut(s) 57, 191, 241, 273, 301
MspR9I CCNGG 4 cut(s) 241, 302, 361, 704
MvaI CCWGG 2 cut(s) 361, 704
MvnI CGCG 3 cut(s) 87, 216, 260
MwoI GCNNNNNNNGC 1 cut(s) 578
NciI CCSGG 2 cut(s) 241, 302
NdeII GATC 3 cut(s) 346, 709, 1030
NlaIII CATG 5 cut(s) 74, 962, 974, 1115, 1161
NlaIV GGNNCC 5 cut(s) 231, 305, 711, 1007, 1131
NmeAIII GCCGAG 3 cut(s) 93, 130, 570
NmuCI GTSAC 4 cut(s) 160, 533, 628, 810
NspV TTCGAA 1 cut(s) 1138
PagI TCATGA 1 cut(s) 970
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 8 cut(s) 236, 269, 276, 334, 356, 469, 974, 1115
PflFI GACNNNGTC 2 cut(s) 92, 302
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 7 cut(s) 44, 47, 119, 124, 294, 787, 988
PpuMI RGGWCCY 1 cut(s) 304
PsiI TTATAA 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 304
Psp6I CCWGG 2 cut(s) 359, 702
PspGI CCWGG 2 cut(s) 359, 702
PspN4I GGNNCC 5 cut(s) 231, 305, 711, 1007, 1131
PspPI GGNCC 7 cut(s) 188, 208, 298, 304, 339, 686, 1147
PspPPI RGGWCCY 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 3 cut(s) 346, 709, 1030
PsyI GACNNNGTC 2 cut(s) 92, 302
PvuII CAGCTG 1 cut(s) 909
RsaI GTAC 1 cut(s) 897
RsaNI GTAC 1 cut(s) 896
SaqAI TTAA 2 cut(s) 762, 1152
SatI GCNGC 7 cut(s) 43, 46, 118, 123, 293, 786, 987
Sau3AI GATC 3 cut(s) 346, 709, 1030
Sau96I GGNCC 7 cut(s) 188, 208, 298, 304, 339, 686, 1147
ScrFI CCNGG 4 cut(s) 241, 302, 361, 704
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 1 cut(s) 772
SfuI TTCGAA 1 cut(s) 1138
SinI GGWCC 4 cut(s) 208, 298, 304, 1147
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
Sse9I AATT 3 cut(s) 549, 965, 1134
SsiI CCGC 7 cut(s) 43, 85, 117, 214, 313, 572, 752
StyD4I CCNGG 4 cut(s) 239, 300, 359, 702
StyI CCWWGG 1 cut(s) 1001
TaaI ACNGT 6 cut(s) 406, 415, 445, 820, 900, 1144
TaiI ACGT 4 cut(s) 78, 144, 597, 646
TaqI TCGA 3 cut(s) 147, 846, 1138
TaqII GACCGA 1 cut(s) 196
TasI AATT 3 cut(s) 549, 965, 1134
TauI GCSGC 2 cut(s) 45, 120
TfiI GAWTC 8 cut(s) 236, 269, 276, 334, 356, 469, 974, 1115
Tru1I TTAA 2 cut(s) 762, 1152
Tru9I TTAA 2 cut(s) 762, 1152
TscAI CASTG 5 cut(s) 448, 819, 905, 1062, 1149
TseFI GTSAC 4 cut(s) 160, 533, 628, 810
TseI GCWGC 5 cut(s) 45, 122, 292, 785, 986
Tsp45I GTSAC 4 cut(s) 160, 533, 628, 810
TspDTI ATGAA 4 cut(s) 959, 959, 975, 987
TspGWI ACGGA 2 cut(s) 148, 1108
TspRI CASTG 5 cut(s) 448, 819, 905, 1062, 1149
Tth111I GACNNNGTC 2 cut(s) 92, 302
VpaK11BI GGWCC 4 cut(s) 208, 298, 304, 1147
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.