Rh5CG496400

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003783
Physical Location & Seq
Forward (+)
69083738 .. 69085623
1886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG496400.1

Sequence Viewer

Length: 1158 bp
ATGGGGAGAAACCGAACCCAGCCCACTTCCTCCCCTCGCCGGTGCCTGATTGCCGGAAACTACTGCCACGACGTTCTCATCCGCGACGGCGTCGTTTTGGCAGAGACGCTCGGCGGGGCTGCTTCTTTCATCTCCAACGTGTTCGATGGCGTGTCCGTGAGTTACAATCTGGTGTCCAAAGTCGGGTCCGATTTTGCTTACTCGGTCCACCGCGACCCGATTGTGGTACCTGATTGCAGGACGACTCTGTTCCACGCGCATTTTGATTCTGGAATCGACGGGGAGGGCCAGCATGATCGGGTTCTGAAGCGGGTCAATGCCTGCGACCCGATTCGCCCCTCGGATCTGCCCGAATCCAGGTTTGATTTCGGAATGGCGGTTGGGGTCGGCGGCGAGATTACGGTGGAGACGGTTGAGAAACTGGTGGAGATTTGCAGCACTGTGTTTGTGGACATTCAGGCTCTGATTCGGGTTTTTGATGAGGTTGATGGGGCTGTGAAGCTTGTGGGTTTGAAAGAGAGTGGGTTCTTTCACCTCTTGCCCAGAATTGGGTTTCTAAAGGCGTCGGCGGAGGAGGCTCTGTTTATGGACGTGGAGGAGGTCAGGAAGGTGTGTTGTGTTGTGGTGACGAATGGGAAGCGTGGGTGTAGAGTGTATTGGAGAGATGGGGAAGTAGAAGTCGGGCCGTTTCCGACCAGCCAGGTTGATCCAACCGGTGCCGGAGATAGTTTTCTCGGTGGGTTTGTTGCGGGGCTTGTTAATGGGTTGGCTGTGCCTGATGCTGCATTGCTTGGGAACTTATTCGGGTCATTGACTGTGGGGCAAATCGGACTGCCCAAGTTCGACTTGAGGTTGTTACAGAGAGTTCAGGAAGAGGTGCAGAAGAGAAAGGTACAGTGTATCAGCTGCCACGAAAGAAAAGACGAGAAGTTCAGGTTTATGAATGTAGCAGGACATGAACAGTTTCATGCATCCCTCATAGCAGCTAAACAGATACCAAGGTGCCTTCCTCGTGAATGTGAATGGGATCTGCCAAATTCTCCTTCCAGAGCAGTGGAGCAGGGTTTGAATGTCCTCCCCCATTTCACAACCCAGCCGAAACTTTTACCCGATTCCGTCTACGAGGAGCCAATTCGAACAGCGGATGGTAAACAATGA

Protein Analysis

385

Amino Acids

42.22

Weight (kDa)

5.74

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 206 - 277 4.2e-19 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 4 cut(s) 42, 226, 716, 1002
AccI GTMKAC 1 cut(s) 1119
AccII CGCG 3 cut(s) 84, 213, 257
AciI CCGC 9 cut(s) 82, 114, 211, 310, 377, 390, 569, 749, 1142
AclWI GGATC 3 cut(s) 351, 701, 1035
AcsI RAATTY 1 cut(s) 1036
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 2 cut(s) 90, 563
AfaI GTAC 2 cut(s) 228, 894
AfiI CCNNNNNNNGG 6 cut(s) 39, 183, 223, 357, 548, 549
AflIII ACRYGT 1 cut(s) 138
AgeI ACCGGT 1 cut(s) 713
AgsI TTSAA 2 cut(s) 514, 1069
AjiI CACGTC 1 cut(s) 592
AjnI CCWGG 2 cut(s) 356, 699
AjuI GAANNNNNNNTTGG 2 cut(s) 1027, 1059
AluBI AGCT 3 cut(s) 502, 906, 986
AluI AGCT 3 cut(s) 502, 906, 986
Alw26I GTCTC 2 cut(s) 98, 401
AlwI GGATC 3 cut(s) 351, 701, 1035
AlwNI CAGNNNCTG 1 cut(s) 463
AoxI GGCC 2 cut(s) 286, 683
ApeKI GCWGC 5 cut(s) 119, 435, 782, 906, 983
ApoI RAATTY 1 cut(s) 1036
AsiGI ACCGGT 1 cut(s) 713
Asp700I GAANNNNTTC 2 cut(s) 800, 963
Asp718I GGTACC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 4 cut(s) 186, 205, 286, 683
AsuHPI GGTGA 2 cut(s) 524, 637
AsuII TTCGAA 1 cut(s) 1135
AvaII GGWCC 2 cut(s) 186, 205
BaeI ACNNNNGTAYC 2 cut(s) 986, 1019
BanI GGYRCC 4 cut(s) 42, 226, 716, 1002
BauI CACGAG 1 cut(s) 1011
BbvI GCAGC 5 cut(s) 106, 447, 769, 893, 995
BccI CCATC 4 cut(s) 140, 482, 659, 1139
BceAI ACGGC 2 cut(s) 103, 670
BciT130I CCWGG 2 cut(s) 358, 701
BcoDI GTCTC 2 cut(s) 98, 401
BisI GCNGC 6 cut(s) 120, 391, 436, 783, 907, 984
BlsI GCNGC 6 cut(s) 121, 392, 437, 784, 908, 985
Bme1390I CCNGG 2 cut(s) 358, 701
Bme18I GGWCC 2 cut(s) 186, 205
BmgBI CACGTC 1 cut(s) 592
BmgT120I GGNCC 4 cut(s) 186, 205, 286, 683
BmiI GGNNCC 6 cut(s) 44, 187, 228, 718, 1004, 1128
BmrFI CCNGG 2 cut(s) 358, 701
BmsI GCATC 2 cut(s) 769, 980
Bpu14I TTCGAA 1 cut(s) 1135
BpuEI CTTGAG 1 cut(s) 868
BsaHI GRCGYC 2 cut(s) 90, 563
BsaJI CCNNGG 2 cut(s) 339, 998
BsaWI WCCGGW 1 cut(s) 713
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 6 cut(s) 39, 183, 223, 357, 548, 549
Bse118I RCCGGY 2 cut(s) 39, 713
Bse1I ACTGG 1 cut(s) 426
Bse3DI GCAATG 1 cut(s) 785
BseBI CCWGG 2 cut(s) 358, 701
BseDI CCNNGG 2 cut(s) 339, 998
BseGI GGATG 3 cut(s) 78, 971, 1150
BseLI CCNNNNNNNGG 6 cut(s) 39, 183, 223, 357, 548, 549
BseMI GCAATG 1 cut(s) 785
BseNI ACTGG 1 cut(s) 426
BseRI GAGGAG 3 cut(s) 587, 611, 1139
BseXI GCAGC 5 cut(s) 106, 447, 769, 893, 995
BseYI CCCAGC 2 cut(s) 18, 1092
BsgI GTGCAG 1 cut(s) 899
Bsh1236I CGCG 3 cut(s) 84, 213, 257
BshFI GGCC 2 cut(s) 288, 685
BshNI GGYRCC 4 cut(s) 42, 226, 716, 1002
BshTI ACCGGT 1 cut(s) 713
BsiSI CCGG 4 cut(s) 40, 54, 714, 720
BslI CCNNNNNNNGG 6 cut(s) 39, 183, 223, 357, 548, 549
BsmAI GTCTC 2 cut(s) 98, 401
BsmBI CGTCTC 2 cut(s) 98, 401
BsnI GGCC 2 cut(s) 288, 685
Bsp119I TTCGAA 1 cut(s) 1135
Bsp143I GATC 4 cut(s) 295, 343, 706, 1027
BspACI CCGC 9 cut(s) 82, 114, 211, 310, 377, 390, 569, 749, 1142
BspANI GGCC 2 cut(s) 288, 685
BspFNI CGCG 3 cut(s) 84, 213, 257
BspLI GGNNCC 6 cut(s) 44, 187, 228, 718, 1004, 1128
BspPI GGATC 3 cut(s) 351, 701, 1035
BspT104I TTCGAA 1 cut(s) 1135
BspT107I GGYRCC 4 cut(s) 42, 226, 716, 1002
BsrDI GCAATG 1 cut(s) 785
BsrFI RCCGGY 2 cut(s) 39, 713
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 2 cut(s) 39, 713
BssECI CCNNGG 2 cut(s) 339, 998
BssMI GATC 4 cut(s) 295, 343, 706, 1027
BssNI GRCGYC 2 cut(s) 90, 563
BssSI CACGAG 1 cut(s) 1011
BssT1I CCWWGG 1 cut(s) 998
Bst2BI CACGAG 1 cut(s) 1011
Bst2UI CCWGG 2 cut(s) 358, 701
Bst4CI ACNGT 6 cut(s) 403, 412, 442, 817, 897, 963
Bst6I CTCTTC 2 cut(s) 867, 878
BstACI GRCGYC 2 cut(s) 90, 563
BstBI TTCGAA 1 cut(s) 1135
BstC8I GCNNGC 2 cut(s) 290, 322
BstF5I GGATG 3 cut(s) 78, 971, 1150
BstFNI CGCG 3 cut(s) 84, 213, 257
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 4 cut(s) 298, 346, 709, 1030
BstMAI GTCTC 2 cut(s) 98, 401
BstMBI GATC 4 cut(s) 295, 343, 706, 1027
BstMWI GCNNNNNNNGC 1 cut(s) 575
BstNI CCWGG 2 cut(s) 358, 701
BstSCI CCNGG 2 cut(s) 356, 699
BstUI CGCG 3 cut(s) 84, 213, 257
BstV1I GCAGC 5 cut(s) 106, 447, 769, 893, 995
BstX2I RGATCY 2 cut(s) 343, 1027
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 2 cut(s) 343, 1027
BsuRI GGCC 2 cut(s) 288, 685
BtrI CACGTC 1 cut(s) 592
BtsCI GGATG 3 cut(s) 78, 971, 1150
BtsI GCAGTG 1 cut(s) 1059
BtsIMutI CAGTG 3 cut(s) 438, 902, 1059
Cac8I GCNNGC 2 cut(s) 290, 322
CaiI CAGNNNCTG 1 cut(s) 463
CfoI GCGC 1 cut(s) 259
Cfr10I RCCGGY 2 cut(s) 39, 713
Cfr13I GGNCC 4 cut(s) 186, 205, 286, 683
CseI GACGC 3 cut(s) 79, 115, 552
Csp6I GTAC 2 cut(s) 227, 893
CspAI ACCGGT 1 cut(s) 713
CviAII CATG 3 cut(s) 293, 956, 968
CviQI GTAC 2 cut(s) 227, 893
DpnI GATC 4 cut(s) 297, 345, 708, 1029
DpnII GATC 4 cut(s) 295, 343, 706, 1027
Eam1104I CTCTTC 2 cut(s) 867, 878
EarI CTCTTC 2 cut(s) 867, 878
EciI GGCGGA 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 998
Eco47I GGWCC 2 cut(s) 186, 205
Eco57I CTGAAG 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 356, 699
EcoT14I CCWWGG 1 cut(s) 998
EcoT22I ATGCAT 1 cut(s) 973
ErhI CCWWGG 1 cut(s) 998
Esp3I CGTCTC 2 cut(s) 98, 401
FaeI CATG 3 cut(s) 296, 959, 971
FaiI YATR 6 cut(s) 294, 587, 941, 957, 969, 980
FalI AAGNNNNNCTT 2 cut(s) 830, 862
FatI CATG 3 cut(s) 292, 955, 967
FauI CCCGC 3 cut(s) 107, 303, 742
FblI GTMKAC 1 cut(s) 1119
Fnu4HI GCNGC 6 cut(s) 120, 391, 436, 783, 907, 984
FokI GGATG 2 cut(s) 65, 958
Fsp4HI GCNGC 6 cut(s) 120, 391, 436, 783, 907, 984
GlaI GCGC 1 cut(s) 258
GluI GCNGC 6 cut(s) 120, 391, 436, 783, 907, 984
GsaI CCCAGC 2 cut(s) 22, 1096
HaeIII GGCC 2 cut(s) 288, 685
HapII CCGG 4 cut(s) 40, 54, 714, 720
HgaI GACGC 3 cut(s) 79, 115, 552
HhaI GCGC 1 cut(s) 259
Hin1I GRCGYC 2 cut(s) 90, 563
Hin1II CATG 3 cut(s) 296, 959, 971
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 7 cut(s) 244, 266, 273, 331, 353, 466, 1112
HpaII CCGG 4 cut(s) 40, 54, 714, 720
HphI GGTGA 2 cut(s) 524, 637
Hpy166II GTNNAC 4 cut(s) 208, 451, 1120, 1151
Hpy188I TCNGA 7 cut(s) 190, 306, 343, 371, 465, 693, 830
Hpy188III TCNNGA 5 cut(s) 270, 604, 869, 1013, 1047
Hpy8I GTNNAC 4 cut(s) 208, 451, 1120, 1151
Hpy99I CGWCG 5 cut(s) 74, 89, 95, 281, 568
HpyAV CCTTC 3 cut(s) 601, 1016, 1053
HpyCH4III ACNGT 6 cut(s) 403, 412, 442, 817, 897, 963
HpyCH4IV ACGT 3 cut(s) 72, 138, 591
HpyCH4V TGCA 5 cut(s) 237, 435, 785, 880, 971
HpyF10VI GCNNNNNNNGC 1 cut(s) 575
HpySE526I ACGT 3 cut(s) 72, 138, 591
Hsp92I GRCGYC 2 cut(s) 90, 563
Hsp92II CATG 3 cut(s) 296, 959, 971
HspAI GCGC 1 cut(s) 257
KpnI GGTACC 1 cut(s) 230
Kzo9I GATC 4 cut(s) 295, 343, 706, 1027
LmnI GCTCC 2 cut(s) 1057, 1126
Lsp1109I GCAGC 5 cut(s) 106, 447, 769, 893, 995
LweI GCATC 2 cut(s) 769, 980
MaeII ACGT 3 cut(s) 72, 138, 591
MaeIII GTNAC 3 cut(s) 161, 625, 855
MalI GATC 4 cut(s) 297, 345, 708, 1029
MboI GATC 4 cut(s) 295, 343, 706, 1027
MboII GAAGA 2 cut(s) 884, 895
MflI RGATCY 2 cut(s) 343, 1027
MluCI AATT 3 cut(s) 546, 1036, 1131
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 3 cut(s) 159, 716, 734
Mph1103I ATGCAT 1 cut(s) 973
MroXI GAANNNNTTC 2 cut(s) 800, 963
MseI TTAA 1 cut(s) 759
MspA1I CMGCKG 2 cut(s) 906, 1142
MspI CCGG 4 cut(s) 40, 54, 714, 720
MspR9I CCNGG 2 cut(s) 358, 701
MvaI CCWGG 2 cut(s) 358, 701
MvnI CGCG 3 cut(s) 84, 213, 257
MwoI GCNNNNNNNGC 1 cut(s) 575
NdeII GATC 4 cut(s) 295, 343, 706, 1027
NlaIII CATG 3 cut(s) 296, 959, 971
NlaIV GGNNCC 6 cut(s) 44, 187, 228, 718, 1004, 1128
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 625
NsiI ATGCAT 1 cut(s) 973
NspV TTCGAA 1 cut(s) 1135
PdmI GAANNNNTTC 2 cut(s) 800, 963
PfeI GAWTC 6 cut(s) 266, 273, 331, 353, 466, 1112
PflFI GACNNNGTC 1 cut(s) 89
PinAI ACCGGT 1 cut(s) 713
PkrI GCNGC 6 cut(s) 121, 392, 437, 784, 908, 985
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
Psp6I CCWGG 2 cut(s) 356, 699
PspFI CCCAGC 2 cut(s) 18, 1092
PspGI CCWGG 2 cut(s) 356, 699
PspN4I GGNNCC 6 cut(s) 44, 187, 228, 718, 1004, 1128
PspPI GGNCC 4 cut(s) 186, 205, 286, 683
PstNI CAGNNNCTG 1 cut(s) 463
PsuI RGATCY 2 cut(s) 343, 1027
PsyI GACNNNGTC 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 906
RsaI GTAC 2 cut(s) 228, 894
RsaNI GTAC 2 cut(s) 227, 893
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 6 cut(s) 120, 391, 436, 783, 907, 984
Sau3AI GATC 4 cut(s) 295, 343, 706, 1027
Sau96I GGNCC 4 cut(s) 186, 205, 286, 683
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 358, 701
SfaNI GCATC 2 cut(s) 769, 980
SfuI TTCGAA 1 cut(s) 1135
SgrAI CRCCGGYG 1 cut(s) 39
SinI GGWCC 2 cut(s) 186, 205
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
Sse9I AATT 3 cut(s) 546, 1036, 1131
SsiI CCGC 9 cut(s) 82, 114, 211, 310, 377, 390, 569, 749, 1142
StyD4I CCNGG 2 cut(s) 356, 699
StyI CCWWGG 1 cut(s) 998
TaaI ACNGT 6 cut(s) 403, 412, 442, 817, 897, 963
TaiI ACGT 3 cut(s) 75, 141, 594
TaqI TCGA 4 cut(s) 144, 276, 843, 1135
TaqII GACCGA 1 cut(s) 193
TasI AATT 3 cut(s) 546, 1036, 1131
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 6 cut(s) 266, 273, 331, 353, 466, 1112
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 3 cut(s) 445, 902, 1059
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 5 cut(s) 119, 435, 782, 906, 983
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 4 cut(s) 118, 956, 956, 972
TspGWI ACGGA 2 cut(s) 145, 1105
TspRI CASTG 3 cut(s) 445, 902, 1059
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 186, 205
XapI RAATTY 1 cut(s) 1036
XmiI GTMKAC 1 cut(s) 1119
XmnI GAANNNNTTC 2 cut(s) 800, 963
Zsp2I ATGCAT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.