Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003783

Physical Location & Seq

Genomic Coordinates Reverse (-)

5773425 .. 5775033

Gene Structure 1609 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1070700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1155 bp

ATGGGTCGAGGCCCGAATCAATCCAATTCAGCTCCCCGGGTCCTAATCGCCGGCAACTACTGCCACGACGTTTTATTCAAAGACGGCGTCGTTTTGGCCGAGACTCCCGGCGGGGCCGCCTCCTTCATCTCCAATGTCTTCGATGGAGTTTCCGTTCCCTACAATTTGGTGTCCAAAGTCGGGTCGGACTTCGCTTACTCGGTCCCGCGTGACCCGATTGTTGTACCCGATTGCAATACCACTCTCTTCCACGCGCATTTTGATTCCGGCGTCGGCGGTGACGAACGGCGTGACCGGGTTCTGCAACGGGTAAGAGCTTGCGGCCCGATTAGGCCCTCGGATCTCCCCGGATACAGATTTGATTTCGGAATGGCTGTCGGTGTTGGCGGCGAGATCACCGTTGAGACGGTGGAGAAGTTGGTTGAGACTTGCAGCACTGTGTTTGTGGACATTCAGGCTCTGATTCGAGCTTTCGACGGAGTCGACGGGACCGTGAAGCTTGTGGGTTTGAAGGAGAGTGGCTTCTTCCACCTCTTGCCTCGAATTGGGTTTTTGAAGGCTTCGGCGGAGGAGGCGTTCTTTATGGAGGTGGAGGAGGTGAGGAAGCTCTGCTGTGTTGTGGTGACGAACGGGAGGCGTGGGTGTAAAGTTTATTGGGGAGACGGCGAGGTGGAAGTTGGGCCTTTTCCGACGCACCAGGTTGATCCGACGGGCGCAGGAGACAGTTTCCTTGGTGGGTTTGTTGCCGGACTTGTTAATGGCTTGACTGTGCCTGATGCTGCATTGCTCGGCAACCTTTTCGGGTCACTGACTGTTGGGCATATTGGATTGCCCAAGTTTGATTCCAAGTTATTGCAGAGAGTTCGTGAAGAGGTGCAGAGGAGAAAAATGCAGTGCGGTAATTGCCGCGAAAGAAAAGATGACAGGTTTAGGTTTGTGAACATAGCAGGACATGAGCAATTTCATGCATCTCTAGGGGCAGCTAAGCAGACAACAAAGTGCCTTGCTCAGGAACCTCAATGGGATCTGCCAAGTTCTCCTCCCAAGTCAATGGAACGGGGTGTCCTCCCTCAATACGCGACACAGCCTAAATTGTTACCGGTCTCTGTCAATGAAGAACCAGTTCAAATCGTTGACACTGCCGGTAAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006066 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010264 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0019140 GO:0019637 GO:0019751 GO:0032958 GO:0033517 GO:0043647 GO:0044237 GO:0044281 GO:0044283 GO:0046165 GO:0046173 GO:0071704 GO:0090407 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 map00562 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

385

Amino Acids

41.58

Weight (kDa)

6.1

Isoelectric Point (pI)

31.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	206 - 276	2.4e-18	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003783)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58730
fragaria_vesca	FvH4_3g38550
malus_domestica	MD03G1070700.v1.1 MD11G1074700.v1.1
prunus_persica	Prupe.6G057500_v2.0.a1
pyrus_communis	pycom03g05690 pycom11g06200
rosa_chinensis	RchiOBHm_Chr5g0069451 RchiOBHm_Chr6g0276161 RchiOBHm_Chr6g0303391 RchiOBHm_Chr7g0201761
rosa_laevigata	RLG00000036054
rosa_multiflora	Rmu_sc0002658.1_g000033 Rmu_sc0004181.1_g000022 Rmu_sc0004359.1_g000001 Rmu_sc0006422.1_g000024 Rmu_sc0007219.1_g000001 Rmu_sc0010739.1_g000002 Rmu_sc0011424.1_g000019 Rmu_sc0019422.1_g000003 Rmu_sc0022597.1_g000008 Rmu_ssc0000201.1_g000003
rosa_roxburghii	Rroxscaffold_1G00011500 Rroxscaffold_2G00112290
rosa_rugosa	Rorug05G0397700
rosa_samantha	Rh5AG455000 Rh5BG474000 Rh5CG496400 Rh5DG485800 Rh6DG206800 Rh7AG156400 Rh7DG198700
rosa_wichuraiana	Rw2G037450 Rw5G042440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	483
AccII	CGCG	4 cut(s)	208, 254, 909, 1079
AciI	CCGC	9 cut(s)	111, 117, 206, 276, 321, 387, 566, 897, 907
AclWI	GGATC	3 cut(s)	348, 698, 1032
AcoI	YGGCCR	1 cut(s)	96
AcyI	GRCGYC	2 cut(s)	87, 270
AfaI	GTAC	1 cut(s)	225
AfiI	CCNNNNNNNGG	3 cut(s)	180, 545, 1009
AgeI	ACCGGT	1 cut(s)	1099
AgsI	TTSAA	4 cut(s)	79, 511, 556, 1127
AjnI	CCWGG	1 cut(s)	696
AloI	GAACNNNNNNTCC	6 cut(s)	138, 170, 560, 592, 1047, 1079
AluBI	AGCT	6 cut(s)	32, 317, 470, 499, 607, 983
AluI	AGCT	6 cut(s)	32, 317, 470, 499, 607, 983
Alw26I	GTCTC	6 cut(s)	95, 398, 419, 654, 714, 1108
AlwI	GGATC	3 cut(s)	348, 698, 1032
AlwNI	CAGNNNCTG	1 cut(s)	460
Ama87I	CYCGRG	1 cut(s)	36
AoxI	GGCC	6 cut(s)	10, 96, 114, 322, 332, 680
ApeKI	GCWGC	3 cut(s)	432, 779, 980
ArsI	GACNNNNNNTTYG	2 cut(s)	72, 104
AsiGI	ACCGGT	1 cut(s)	1099
Asp700I	GAANNNNTTC	1 cut(s)	1122
AspLEI	GCGC	2 cut(s)	256, 716
AspS9I	GGNCC	8 cut(s)	11, 40, 114, 202, 323, 333, 489, 680
AsuC2I	CCSGG	5 cut(s)	37, 38, 108, 296, 348
AsuHPI	GGTGA	4 cut(s)	290, 388, 610, 634
AvaI	CYCGRG	1 cut(s)	36
AvaII	GGWCC	3 cut(s)	40, 202, 489
BbsI	GAAGAC	1 cut(s)	130
BbvI	GCAGC	3 cut(s)	444, 766, 992
BccI	CCATC	1 cut(s)	137
BceAI	ACGGC	3 cut(s)	100, 302, 679
BcgI	CGANNNNNNTGC	4 cut(s)	781, 815, 845, 879
BciT130I	CCWGG	1 cut(s)	698
BciVI	GTATCC	1 cut(s)	344
BcnI	CCSGG	5 cut(s)	37, 38, 108, 296, 348
BcoDI	GTCTC	6 cut(s)	95, 398, 419, 654, 714, 1108
BfaI	CTAG	1 cut(s)	974
BfuI	GTATCC	1 cut(s)	344
BisI	GCNGC	7 cut(s)	117, 322, 388, 433, 780, 907, 981
BlpI	GCTNAGC	1 cut(s)	984
BlsI	GCNGC	7 cut(s)	118, 323, 389, 434, 781, 908, 982
Bme1390I	CCNGG	6 cut(s)	37, 38, 108, 296, 348, 698
Bme18I	GGWCC	3 cut(s)	40, 202, 489
BmeT110I	CYCGRG	1 cut(s)	36
BmgT120I	GGNCC	8 cut(s)	11, 40, 114, 202, 323, 333, 489, 680
BmiI	GGNNCC	5 cut(s)	41, 115, 204, 490, 1014
BmrFI	CCNGG	6 cut(s)	37, 38, 108, 296, 348, 698
BmsI	GCATC	2 cut(s)	766, 977
BpiI	GAAGAC	1 cut(s)	130
Bpu10I	CCTNAGC	1 cut(s)	1008
Bpu1102I	GCTNAGC	1 cut(s)	984
BpuMI	CCSGG	5 cut(s)	37, 38, 108, 296, 348
BsaHI	GRCGYC	2 cut(s)	87, 270
BsaI	GGTCTC	1 cut(s)	1108
BsaJI	CCNNGG	5 cut(s)	35, 36, 336, 346, 730
BsaWI	WCCGGW	1 cut(s)	1099
BsaXI	ACNNNNNCTCC	4 cut(s)	138, 168, 560, 590
Bsc4I	CCNNNNNNNGG	3 cut(s)	180, 545, 1009
Bse118I	RCCGGY	3 cut(s)	50, 1099, 1142
Bse1I	ACTGG	1 cut(s)	1121
Bse3DI	GCAATG	1 cut(s)	782
BseBI	CCWGG	1 cut(s)	698
BseDI	CCNNGG	5 cut(s)	35, 36, 336, 346, 730
BseLI	CCNNNNNNNGG	3 cut(s)	180, 545, 1009
BseMI	GCAATG	1 cut(s)	782
BseMII	CTCAG	1 cut(s)	1022
BseNI	ACTGG	1 cut(s)	1121
BseRI	GAGGAG	4 cut(s)	584, 608, 895, 1029
BseXI	GCAGC	3 cut(s)	444, 766, 992
BsgI	GTGCAG	1 cut(s)	896
Bsh1236I	CGCG	4 cut(s)	208, 254, 909, 1079
BshFI	GGCC	6 cut(s)	12, 98, 116, 324, 334, 682
BshTI	ACCGGT	1 cut(s)	1099
BsiHKCI	CYCGRG	1 cut(s)	36
BsiSI	CCGG	9 cut(s)	37, 51, 108, 267, 295, 348, 747, 1100, 1143
BslFI	GGGAC	2 cut(s)	188, 502
BslI	CCNNNNNNNGG	3 cut(s)	180, 545, 1009
BsmAI	GTCTC	6 cut(s)	95, 398, 419, 654, 714, 1108
BsmBI	CGTCTC	2 cut(s)	398, 654
BsmFI	GGGAC	2 cut(s)	188, 502
BsnI	GGCC	6 cut(s)	12, 98, 116, 324, 334, 682
Bso31I	GGTCTC	1 cut(s)	1108
BsoBI	CYCGRG	1 cut(s)	36
Bsp143I	GATC	4 cut(s)	340, 393, 703, 1024
Bsp1720I	GCTNAGC	1 cut(s)	984
BspACI	CCGC	9 cut(s)	111, 117, 206, 276, 321, 387, 566, 897, 907
BspANI	GGCC	6 cut(s)	12, 98, 116, 324, 334, 682
BspCNI	CTCAG	1 cut(s)	1021
BspFNI	CGCG	4 cut(s)	208, 254, 909, 1079
BspLI	GGNNCC	5 cut(s)	41, 115, 204, 490, 1014
BspPI	GGATC	3 cut(s)	348, 698, 1032
BspTNI	GGTCTC	1 cut(s)	1108
BsrDI	GCAATG	1 cut(s)	782
BsrFI	RCCGGY	3 cut(s)	50, 1099, 1142
BsrI	ACTGG	1 cut(s)	1121
BssAI	RCCGGY	3 cut(s)	50, 1099, 1142
BssECI	CCNNGG	5 cut(s)	35, 36, 336, 346, 730
BssMI	GATC	4 cut(s)	340, 393, 703, 1024
BssNI	GRCGYC	2 cut(s)	87, 270
BssT1I	CCWWGG	1 cut(s)	730
Bst2UI	CCWGG	1 cut(s)	698
Bst4CI	ACNGT	7 cut(s)	400, 409, 439, 493, 725, 769, 814
Bst6I	CTCTTC	2 cut(s)	251, 864
BstACI	GRCGYC	2 cut(s)	87, 270
BstAPI	GCANNNNNTGC	1 cut(s)	60
BstC8I	GCNNGC	2 cut(s)	52, 319
BstDEI	CTNAG	2 cut(s)	984, 1008
BstENI	CCTNNNNNAGG	1 cut(s)	1007
BstFNI	CGCG	4 cut(s)	208, 254, 909, 1079
BstHHI	GCGC	2 cut(s)	256, 716
BstKTI	GATC	4 cut(s)	343, 396, 706, 1027
BstMAI	GTCTC	6 cut(s)	95, 398, 419, 654, 714, 1108
BstMBI	GATC	4 cut(s)	340, 393, 703, 1024
BstMWI	GCNNNNNNNGC	3 cut(s)	60, 572, 903
BstNI	CCWGG	1 cut(s)	698
BstSCI	CCNGG	6 cut(s)	35, 36, 106, 294, 346, 696
BstUI	CGCG	4 cut(s)	208, 254, 909, 1079
BstV1I	GCAGC	3 cut(s)	444, 766, 992
BstV2I	GAAGAC	1 cut(s)	130
BstX2I	RGATCY	2 cut(s)	340, 1024
BstXI	CCANNNNNNTGG	1 cut(s)	1051
BstYI	RGATCY	2 cut(s)	340, 1024
BsuI	GTATCC	1 cut(s)	344
BsuRI	GGCC	6 cut(s)	12, 98, 116, 324, 334, 682
BtsI	GCAGTG	2 cut(s)	899, 1137
BtsIMutI	CAGTG	4 cut(s)	435, 806, 899, 1137
Cac8I	GCNNGC	2 cut(s)	52, 319
CaiI	CAGNNNCTG	1 cut(s)	460
CfoI	GCGC	2 cut(s)	256, 716
Cfr10I	RCCGGY	3 cut(s)	50, 1099, 1142
Cfr13I	GGNCC	8 cut(s)	11, 40, 114, 202, 323, 333, 489, 680
Cfr9I	CCCGGG	1 cut(s)	36
CseI	GACGC	3 cut(s)	76, 259, 700
CsiI	ACCWGGT	1 cut(s)	696
Csp6I	GTAC	1 cut(s)	224
CspAI	ACCGGT	1 cut(s)	1099
CviAII	CATG	3 cut(s)	953, 965, 1152
CviQI	GTAC	1 cut(s)	224
DdeI	CTNAG	2 cut(s)	984, 1008
DpnI	GATC	4 cut(s)	342, 395, 705, 1026
DpnII	GATC	4 cut(s)	340, 393, 703, 1024
EaeI	YGGCCR	1 cut(s)	96
Eam1104I	CTCTTC	2 cut(s)	251, 864
EarI	CTCTTC	2 cut(s)	251, 864
EciI	GGCGGA	1 cut(s)	581
Eco130I	CCWWGG	1 cut(s)	730
Eco31I	GGTCTC	1 cut(s)	1108
Eco47I	GGWCC	3 cut(s)	40, 202, 489
Eco88I	CYCGRG	1 cut(s)	36
EcoNI	CCTNNNNNAGG	1 cut(s)	1007
EcoO109I	RGGNCCY	2 cut(s)	40, 333
EcoRII	CCWGG	1 cut(s)	696
EcoT14I	CCWWGG	1 cut(s)	730
EcoT22I	ATGCAT	1 cut(s)	970
ErhI	CCWWGG	1 cut(s)	730
Esp3I	CGTCTC	2 cut(s)	398, 654
FaeI	CATG	3 cut(s)	956, 968, 1155
FaiI	YATR	6 cut(s)	584, 822, 944, 954, 966, 1153
FaqI	GGGAC	2 cut(s)	188, 502
FatI	CATG	3 cut(s)	952, 964, 1151
FauI	CCCGC	2 cut(s)	104, 213
FblI	GTMKAC	1 cut(s)	483
Fnu4HI	GCNGC	7 cut(s)	117, 322, 388, 433, 780, 907, 981
Fsp4HI	GCNGC	7 cut(s)	117, 322, 388, 433, 780, 907, 981
FspBI	CTAG	1 cut(s)	974
GlaI	GCGC	2 cut(s)	255, 715
GluI	GCNGC	7 cut(s)	117, 322, 388, 433, 780, 907, 981
HaeIII	GGCC	6 cut(s)	12, 98, 116, 324, 334, 682
HapII	CCGG	9 cut(s)	37, 51, 108, 267, 295, 348, 747, 1100, 1143
HgaI	GACGC	3 cut(s)	76, 259, 700
HhaI	GCGC	2 cut(s)	256, 716
Hin1I	GRCGYC	2 cut(s)	87, 270
Hin1II	CATG	3 cut(s)	956, 968, 1155
Hin6I	GCGC	2 cut(s)	254, 714
HinP1I	GCGC	2 cut(s)	254, 714
HincII	GTYRAC	2 cut(s)	484, 1135
HindII	GTYRAC	2 cut(s)	484, 1135
HindIII	AAGCTT	1 cut(s)	497
HinfI	GANTC	6 cut(s)	16, 103, 263, 463, 480, 842
HpaII	CCGG	9 cut(s)	37, 51, 108, 267, 295, 348, 747, 1100, 1143
HphI	GGTGA	4 cut(s)	290, 388, 610, 634
Hpy166II	GTNNAC	5 cut(s)	448, 484, 940, 1135, 1148
Hpy188I	TCNGA	6 cut(s)	187, 340, 368, 462, 690, 708
Hpy188III	TCNNGA	2 cut(s)	866, 1010
Hpy8I	GTNNAC	5 cut(s)	448, 484, 940, 1135, 1148
Hpy99I	CGWCG	7 cut(s)	71, 92, 275, 479, 488, 694, 712
HpyAV	CCTTC	3 cut(s)	133, 505, 550
HpyCH4III	ACNGT	7 cut(s)	400, 409, 439, 493, 725, 769, 814
HpyCH4IV	ACGT	1 cut(s)	69
HpyCH4V	TGCA	8 cut(s)	234, 304, 432, 782, 856, 877, 892, 968
HpyF10VI	GCNNNNNNNGC	3 cut(s)	60, 572, 903
HpyF3I	CTNAG	2 cut(s)	984, 1008
HpySE526I	ACGT	1 cut(s)	69
Hsp92I	GRCGYC	2 cut(s)	87, 270
Hsp92II	CATG	3 cut(s)	956, 968, 1155
HspAI	GCGC	2 cut(s)	254, 714
KroI	GCCGGC	1 cut(s)	50
KroNI	GCCGGC	1 cut(s)	52
Kzo9I	GATC	4 cut(s)	340, 393, 703, 1024
LmnI	GCTCC	1 cut(s)	37
Lsp1109I	GCAGC	3 cut(s)	444, 766, 992
LweI	GCATC	2 cut(s)	766, 977
MabI	ACCWGGT	1 cut(s)	696
MaeI	CTAG	1 cut(s)	974
MaeII	ACGT	1 cut(s)	69
MaeIII	GTNAC	6 cut(s)	209, 278, 290, 622, 804, 1095
MalI	GATC	4 cut(s)	342, 395, 705, 1026
MboI	GATC	4 cut(s)	340, 393, 703, 1024
MboII	GAAGA	5 cut(s)	130, 238, 517, 881, 1127
MflI	RGATCY	2 cut(s)	340, 1024
MluCI	AATT	6 cut(s)	25, 163, 543, 901, 959, 1091
MlyI	GAGTC	2 cut(s)	97, 489
MmeI	TCCRAC	3 cut(s)	165, 713, 731
Mph1103I	ATGCAT	1 cut(s)	970
MroNI	GCCGGC	1 cut(s)	50
MroXI	GAANNNNTTC	1 cut(s)	1122
MseI	TTAA	1 cut(s)	756
MspI	CCGG	9 cut(s)	37, 51, 108, 267, 295, 348, 747, 1100, 1143
MspR9I	CCNGG	6 cut(s)	37, 38, 108, 296, 348, 698
MvaI	CCWGG	1 cut(s)	698
MvnI	CGCG	4 cut(s)	208, 254, 909, 1079
MwoI	GCNNNNNNNGC	3 cut(s)	60, 572, 903
NaeI	GCCGGC	1 cut(s)	52
NciI	CCSGG	5 cut(s)	37, 38, 108, 296, 348
NdeII	GATC	4 cut(s)	340, 393, 703, 1024
NgoMIV	GCCGGC	1 cut(s)	50
NlaIII	CATG	3 cut(s)	956, 968, 1155
NlaIV	GGNNCC	5 cut(s)	41, 115, 204, 490, 1014
NmeAIII	GCCGAG	2 cut(s)	124, 768
NmuCI	GTSAC	5 cut(s)	209, 278, 290, 622, 804
NsiI	ATGCAT	1 cut(s)	970
PcsI	WCGNNNNNNNCGW	4 cut(s)	96, 279, 480, 489
PdiI	GCCGGC	1 cut(s)	52
PdmI	GAANNNNTTC	1 cut(s)	1122
PfeI	GAWTC	4 cut(s)	16, 263, 463, 842
PflFI	GACNNNGTC	2 cut(s)	86, 479
PinAI	ACCGGT	1 cut(s)	1099
PkrI	GCNGC	7 cut(s)	118, 323, 389, 434, 781, 908, 982
PleI	GAGTC	2 cut(s)	97, 488
PpsI	GAGTC	2 cut(s)	97, 488
PpuMI	RGGWCCY	1 cut(s)	40
Psp5II	RGGWCCY	1 cut(s)	40
Psp6I	CCWGG	1 cut(s)	696
PspGI	CCWGG	1 cut(s)	696
PspN4I	GGNNCC	5 cut(s)	41, 115, 204, 490, 1014
PspPI	GGNCC	8 cut(s)	11, 40, 114, 202, 323, 333, 489, 680
PspPPI	RGGWCCY	1 cut(s)	40
PstNI	CAGNNNCTG	1 cut(s)	460
PsuI	RGATCY	2 cut(s)	340, 1024
PsyI	GACNNNGTC	2 cut(s)	86, 479
RsaI	GTAC	1 cut(s)	225
RsaNI	GTAC	1 cut(s)	224
SalI	GTCGAC	1 cut(s)	482
SaqAI	TTAA	1 cut(s)	756
SatI	GCNGC	7 cut(s)	117, 322, 388, 433, 780, 907, 981
Sau3AI	GATC	4 cut(s)	340, 393, 703, 1024
Sau96I	GGNCC	8 cut(s)	11, 40, 114, 202, 323, 333, 489, 680
SchI	GAGTC	2 cut(s)	97, 489
ScrFI	CCNGG	6 cut(s)	37, 38, 108, 296, 348, 698
SexAI	ACCWGGT	1 cut(s)	696
SfaNI	GCATC	2 cut(s)	766, 977
SinI	GGWCC	3 cut(s)	40, 202, 489
SmaI	CCCGGG	1 cut(s)	38
Sse9I	AATT	6 cut(s)	25, 163, 543, 901, 959, 1091
SsiI	CCGC	9 cut(s)	111, 117, 206, 276, 321, 387, 566, 897, 907
SspMI	CTAG	1 cut(s)	974
StyD4I	CCNGG	6 cut(s)	35, 36, 106, 294, 346, 696
StyI	CCWWGG	1 cut(s)	730
TaaI	ACNGT	7 cut(s)	400, 409, 439, 493, 725, 769, 814
TaiI	ACGT	1 cut(s)	72
TaqI	TCGA	6 cut(s)	7, 141, 466, 474, 483, 541
TaqII	GACCGA	1 cut(s)	190
TasI	AATT	6 cut(s)	25, 163, 543, 901, 959, 1091
TauI	GCSGC	4 cut(s)	119, 324, 390, 909
TfiI	GAWTC	4 cut(s)	16, 263, 463, 842
Tru1I	TTAA	1 cut(s)	756
Tru9I	TTAA	1 cut(s)	756
TscAI	CASTG	4 cut(s)	442, 813, 899, 1144
TseFI	GTSAC	5 cut(s)	209, 278, 290, 622, 804
TseI	GCWGC	3 cut(s)	432, 779, 980
Tsp45I	GTSAC	5 cut(s)	209, 278, 290, 622, 804
TspDTI	ATGAA	3 cut(s)	115, 953, 1128
TspGWI	ACGGA	2 cut(s)	142, 492
TspMI	CCCGGG	1 cut(s)	36
TspRI	CASTG	4 cut(s)	442, 813, 899, 1144
Tth111I	GACNNNGTC	2 cut(s)	86, 479
VpaK11BI	GGWCC	3 cut(s)	40, 202, 489
XagI	CCTNNNNNAGG	1 cut(s)	1007
XmaI	CCCGGG	1 cut(s)	36
XmiI	GTMKAC	1 cut(s)	483
XmnI	GAANNNNTTC	1 cut(s)	1122
XspI	CTAG	1 cut(s)	974
Zsp2I	ATGCAT	1 cut(s)	970

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.