FvH4_3g39360

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009438
Physical Location & Seq
Forward (+)
33487414 .. 33491054
3641 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39360.t1

Sequence Viewer

Length: 1143 bp
ATGGAGAGCGGTGTCACCGGAGCTGACGTCAGCGGAGCCGCCACCAACACCACCCCCACCATAAGCCTCAGTGTCAAGTTCGCCGGCCAGACGATACCCATCAAAAATCTCTCACCGGACTCGACCGTCAAGGACCTCAAGGCTCTTCTTCAACCTCTCACCAATGTCCTCCCTCGCGGCCAAAAGCTCATATCAAAAGGGAAGCTTTTGGTGGATGCAATGACTCTGAGGGAGTCTGAGATCGGTAATGGGGCCAAGATCATGCTCATGGCTTCTCAGGGTTTGCACCAAGGGGATGGACCTATCATTAAAAAAGCTCAGAGTCGCCCAATAATCAGAGAAAACAATGCCGATAAAGGAAAGGCGAAAAAAGCTGCTGTTGATAAGAATAGGATGGAACGATGGAAGGCTACTGGAGTTATAGGACTCAGTGAGTGTAACTTGAAGGCTATACCCGAAGAAGTCTGGGATTGTATAAGTTTTGCAAGAGTTCTGGACCTCAGAAATAATGTTATCCAAGAAGTATCTCCCTACATTGGCTGTATGAGTTTATTGCAGAAATTGTTACTCGATTCAAATGATATATCTGATGACTCAGTTAGCTGGGAAGCACTAAAATCTTTGAAGTCTTTGACTGTTCTTTCTCTGAACCAAAACCGTTTAACTAGATTGCCATCGGATCTGGGCTGGTTGCCATCACTAAGGGAACTTCATCTTGCAAGCAACCAGTTGTCTAGCCTCCCAAGTACTTTCCGATATCTGTCTAAGCTTGAAGTTCTGAAAGTTAACAATAACAGGATCAGCACCATTCCTACATGGATAGGGGACCTTATTTCTCTTATTGAGGTTGATCTTTCATCAAATCTTCTGTCAGATTTGCCCGAGACATTTAGCAATTTGCATAATCTGAAGTCTCTGTCTCTCAGTAATAATGGGCTGAAATCGCTTCCTTCTTCACTATTCAAAATGTGCCTAATGCTCACGAGTCTCGATTTGCACAACACAGAAATAACCATGGATATTCTTCGTCAGATTGAGGGATGGGAAAACTTTGATGAACGACGTCGCTTGAAGCAACAGAAGAAGCTGGATTTTCGCGTTGTCGGCTCTACTGCATTTGATGAAGGTGCTGATAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

381

Amino Acids

41.93

Weight (kDa)

8.89

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 25 - 93 6.1e-14 Ubiquitin family
LRR_8 PF13855 185 - 244 9.1e-09 Leucine rich repeat
LRR_14 PF23598 196 - 285 1e-10 Leucine-rich repeat region
LRR_4 PF12799 209 - 247 2.2e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 232 - 290 7.9e-10 Leucine rich repeat
LRR_4 PF12799 232 - 272 1.1e-07 Leucine Rich repeats (2 copies)
LRR_5 PF13306 248 - 335 6.2e-06 BspA type Leucine rich repeat region (6 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AatII GACGTC 2 cut(s) 30, 1066
AccBSI CCGCTC 1 cut(s) 9
AccII CGCG 2 cut(s) 177, 1098
AciI CCGC 4 cut(s) 9, 33, 39, 177
AclWI GGATC 2 cut(s) 687, 806
AcoI YGGCCR 2 cut(s) 85, 178
AcuI CTGAAG 1 cut(s) 929
AcyI GRCGYC 2 cut(s) 27, 1063
AfaI GTAC 1 cut(s) 748
AfiI CCNNNNNNNGG 1 cut(s) 536
AgsI TTSAA 7 cut(s) 152, 445, 576, 625, 773, 964, 1072
AluBI AGCT 9 cut(s) 23, 187, 205, 317, 374, 603, 769, 1087, 1140
AluI AGCT 9 cut(s) 23, 187, 205, 317, 374, 603, 769, 1087, 1140
Alw26I GTCTC 4 cut(s) 878, 918, 924, 992
AlwI GGATC 2 cut(s) 687, 806
Ama87I CYCGRG 1 cut(s) 881
AoxI GGCC 3 cut(s) 85, 178, 252
ApeKI GCWGC 1 cut(s) 374
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
AspS9I GGNCC 5 cut(s) 133, 252, 299, 496, 826
AsuHPI GGTGA 3 cut(s) 7, 105, 151
AvaI CYCGRG 1 cut(s) 881
AvaII GGWCC 4 cut(s) 133, 299, 496, 826
BauI CACGAG 1 cut(s) 982
BbvI GCAGC 1 cut(s) 361
BccI CCATC 7 cut(s) 107, 290, 388, 396, 682, 703, 1035
BcoDI GTCTC 4 cut(s) 878, 918, 924, 992
BfaI CTAG 2 cut(s) 666, 735
BisI GCNGC 3 cut(s) 39, 178, 375
BlsI GCNGC 3 cut(s) 40, 179, 376
BmcAI AGTACT 1 cut(s) 748
Bme18I GGWCC 4 cut(s) 133, 299, 496, 826
BmeT110I CYCGRG 1 cut(s) 881
BmgT120I GGNCC 5 cut(s) 133, 252, 299, 496, 826
BmiI GGNNCC 3 cut(s) 37, 253, 827
BmsI GCATC 1 cut(s) 205
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 122
BsaBI GATNNNNATC 2 cut(s) 98, 673
BsaHI GRCGYC 2 cut(s) 27, 1063
BsaJI CCNNGG 2 cut(s) 289, 1014
BsaWI WCCGGW 2 cut(s) 17, 115
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 1 cut(s) 536
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 418, 727
Bse3DI GCAATG 1 cut(s) 225
Bse8I GATNNNNATC 2 cut(s) 98, 673
BseDI CCNNGG 2 cut(s) 289, 1014
BseGI GGATG 4 cut(s) 220, 301, 399, 1046
BseJI GATNNNNATC 2 cut(s) 98, 673
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 9 cut(s) 82, 218, 228, 290, 332, 442, 514, 609, 937
BseNI ACTGG 2 cut(s) 418, 727
BseXI GCAGC 1 cut(s) 361
BseYI CCCAGC 1 cut(s) 603
Bsh1236I CGCG 2 cut(s) 177, 1098
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 87, 180, 254
BsiEI CGRYCG 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 881
BsiSI CCGG 3 cut(s) 18, 84, 116
BslFI GGGAC 1 cut(s) 839
BslI CCNNNNNNNGG 1 cut(s) 536
BsmAI GTCTC 4 cut(s) 878, 918, 924, 992
BsmFI GGGAC 1 cut(s) 839
BsnI GGCC 3 cut(s) 87, 180, 254
BsoBI CYCGRG 1 cut(s) 881
Bsp143I GATC 5 cut(s) 240, 258, 679, 798, 850
Bsp19I CCATGG 1 cut(s) 1014
BspACI CCGC 4 cut(s) 9, 33, 39, 177
BspANI GGCC 3 cut(s) 87, 180, 254
BspCNI CTCAG 9 cut(s) 81, 219, 229, 289, 331, 441, 513, 608, 936
BspFNI CGCG 2 cut(s) 177, 1098
BspLI GGNNCC 3 cut(s) 37, 253, 827
BspPI GGATC 2 cut(s) 687, 806
BspQI GCTCTTC 1 cut(s) 150
BsrBI CCGCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 418, 727
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 289, 1014
BssMI GATC 5 cut(s) 240, 258, 679, 798, 850
BssNI GRCGYC 2 cut(s) 27, 1063
BssSI CACGAG 1 cut(s) 982
BssT1I CCWWGG 2 cut(s) 289, 1014
Bst2BI CACGAG 1 cut(s) 982
Bst4CI ACNGT 3 cut(s) 127, 637, 659
Bst6I CTCTTC 1 cut(s) 150
BstACI GRCGYC 2 cut(s) 27, 1063
BstC8I GCNNGC 2 cut(s) 85, 721
BstDSI CCRYGG 1 cut(s) 1014
BstF5I GGATG 4 cut(s) 220, 301, 399, 1046
BstFNI CGCG 2 cut(s) 177, 1098
BstKTI GATC 5 cut(s) 243, 261, 682, 801, 853
BstMAI GTCTC 4 cut(s) 878, 918, 924, 992
BstMBI GATC 5 cut(s) 240, 258, 679, 798, 850
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 3 cut(s) 371, 943, 1104
BstUI CGCG 2 cut(s) 177, 1098
BstV1I GCAGC 1 cut(s) 361
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 1 cut(s) 679
BsuRI GGCC 3 cut(s) 87, 180, 254
BtgI CCRYGG 1 cut(s) 1014
BtsCI GGATG 4 cut(s) 220, 301, 399, 1046
BtsIMutI CAGTG 2 cut(s) 76, 436
Cac8I GCNNGC 2 cut(s) 85, 721
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 5 cut(s) 133, 252, 299, 496, 826
Csp6I GTAC 1 cut(s) 747
CviAII CATG 4 cut(s) 262, 268, 816, 1015
CviQI GTAC 1 cut(s) 747
DpnI GATC 5 cut(s) 242, 260, 681, 800, 852
DpnII GATC 5 cut(s) 240, 258, 679, 798, 850
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 85, 178
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco130I CCWWGG 2 cut(s) 289, 1014
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 4 cut(s) 133, 299, 496, 826
Eco57I CTGAAG 1 cut(s) 929
Eco88I CYCGRG 1 cut(s) 881
EcoO109I RGGNCCY 2 cut(s) 133, 826
EcoRV GATATC 1 cut(s) 758
EcoT14I CCWWGG 2 cut(s) 289, 1014
ErhI CCWWGG 2 cut(s) 289, 1014
FaeI CATG 4 cut(s) 265, 271, 819, 1018
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FaqI GGGAC 1 cut(s) 839
FatI CATG 4 cut(s) 261, 267, 815, 1014
Fnu4HI GCNGC 3 cut(s) 39, 178, 375
FokI GGATG 4 cut(s) 227, 308, 406, 1053
Fsp4HI GCNGC 3 cut(s) 39, 178, 375
FspBI CTAG 2 cut(s) 666, 735
GluI GCNGC 3 cut(s) 39, 178, 375
GsaI CCCAGC 1 cut(s) 607
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 3 cut(s) 87, 180, 254
HapII CCGG 3 cut(s) 18, 84, 116
Hin1I GRCGYC 2 cut(s) 27, 1063
Hin1II CATG 4 cut(s) 265, 271, 819, 1018
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HindIII AAGCTT 2 cut(s) 203, 767
HinfI GANTC 8 cut(s) 119, 223, 233, 322, 426, 572, 593, 985
HpaI GTTAAC 1 cut(s) 787
HpaII CCGG 3 cut(s) 18, 84, 116
HphI GGTGA 3 cut(s) 7, 105, 151
Hpy166II GTNNAC 1 cut(s) 787
Hpy188III TCNNGA 3 cut(s) 494, 982, 989
Hpy8I GTNNAC 1 cut(s) 787
Hpy99I CGWCG 2 cut(s) 1065, 1068
HpyAV CCTTC 4 cut(s) 400, 439, 960, 1118
HpyCH4III ACNGT 3 cut(s) 127, 637, 659
HpyCH4IV ACGT 2 cut(s) 27, 1063
HpyCH4V TGCA 8 cut(s) 218, 286, 485, 556, 719, 901, 997, 1115
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 943, 1104
HpySE526I ACGT 2 cut(s) 27, 1063
Hsp92I GRCGYC 2 cut(s) 27, 1063
Hsp92II CATG 4 cut(s) 265, 271, 819, 1018
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 5 cut(s) 240, 258, 679, 798, 850
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 2 cut(s) 20, 35
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 1 cut(s) 205
MaeI CTAG 2 cut(s) 666, 735
MaeII ACGT 2 cut(s) 27, 1063
MaeIII GTNAC 3 cut(s) 13, 437, 564
MalI GATC 5 cut(s) 242, 260, 681, 800, 852
MbiI CCGCTC 1 cut(s) 9
MboI GATC 5 cut(s) 240, 258, 679, 798, 850
MboII GAAGA 7 cut(s) 137, 140, 470, 857, 945, 1016, 1093
MflI RGATCY 1 cut(s) 679
MluCI AATT 2 cut(s) 560, 895
MlyI GAGTC 7 cut(s) 113, 217, 242, 331, 420, 587, 994
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 3 cut(s) 309, 662, 786
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 3 cut(s) 18, 84, 116
MvnI CGCG 2 cut(s) 177, 1098
MwoI GCNNNNNNNGC 3 cut(s) 371, 943, 1104
NaeI GCCGGC 1 cut(s) 85
NcoI CCATGG 1 cut(s) 1014
NdeII GATC 5 cut(s) 240, 258, 679, 798, 850
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 4 cut(s) 265, 271, 819, 1018
NlaIV GGNNCC 3 cut(s) 37, 253, 827
NmuCI GTSAC 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 572
PkrI GCNGC 3 cut(s) 40, 179, 376
PleI GAGTC 7 cut(s) 113, 217, 241, 330, 420, 587, 993
PpsI GAGTC 7 cut(s) 113, 217, 241, 330, 420, 587, 993
PpuMI RGGWCCY 2 cut(s) 133, 826
Psp5II RGGWCCY 2 cut(s) 133, 826
PspFI CCCAGC 1 cut(s) 603
PspN4I GGNNCC 3 cut(s) 37, 253, 827
PspPI GGNCC 5 cut(s) 133, 252, 299, 496, 826
PspPPI RGGWCCY 2 cut(s) 133, 826
PsuI RGATCY 1 cut(s) 679
RsaI GTAC 1 cut(s) 748
RsaNI GTAC 1 cut(s) 747
RseI CAYNNNNRTG 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 3 cut(s) 309, 662, 786
SatI GCNGC 3 cut(s) 39, 178, 375
Sau3AI GATC 5 cut(s) 240, 258, 679, 798, 850
Sau96I GGNCC 5 cut(s) 133, 252, 299, 496, 826
ScaI AGTACT 1 cut(s) 748
SchI GAGTC 7 cut(s) 113, 217, 242, 331, 420, 587, 994
SfaNI GCATC 1 cut(s) 205
SinI GGWCC 4 cut(s) 133, 299, 496, 826
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 2 cut(s) 560, 895
SsiI CCGC 4 cut(s) 9, 33, 39, 177
SspMI CTAG 2 cut(s) 666, 735
StyI CCWWGG 2 cut(s) 289, 1014
TaaI ACNGT 3 cut(s) 127, 637, 659
TaiI ACGT 2 cut(s) 30, 1066
TaqI TCGA 3 cut(s) 122, 570, 990
TasI AATT 2 cut(s) 560, 895
TatI WGTACW 1 cut(s) 746
TauI GCSGC 2 cut(s) 41, 180
TfiI GAWTC 1 cut(s) 572
Tru1I TTAA 3 cut(s) 309, 662, 786
Tru9I TTAA 3 cut(s) 309, 662, 786
TscAI CASTG 2 cut(s) 76, 436
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 1 cut(s) 374
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 4 cut(s) 701, 846, 1071, 1137
TspRI CASTG 2 cut(s) 76, 436
VpaK11BI GGWCC 4 cut(s) 133, 299, 496, 826
XspI CTAG 2 cut(s) 666, 735
ZraI GACGTC 2 cut(s) 28, 1064
ZrmI AGTACT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.