pycom03g04960

LRR repeats and ubiquitin-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009438
Physical Location & Seq
Reverse (-)
3874271 .. 3877049
2779 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04960.1

Sequence Viewer

Length: 903 bp
ATGGAGCCAAGATCGATGCTCATGGCTTCTCAGGGCTTGCACCAAGGGGACGGACCCATCCTAAAACAAGCGCAGACACGCCCCATTTCCAGATTCAGAATTGTTGATACATTGGGAAGAGAAAAGACGGAAGTTCCTGCTGATAAGAACCGGTTAGATAGATGGAAGGCTACTGGAGTTATAGGACTGTCTGACTACAACTTGAAGGCTATACCTGATGAAGTGTGGGCTTCTGGTACTTCTGCAAGAGTTATTGAACTCAGCAATAATTCTATTCGAGATGTACCTTCAAAGATTGGTTGTCTAAGCTCCTTGCAGAAATTGTTGCTTGCTGCAAATGATATAGTGGATGATTCTATTAGCTGGGAAGGAATAGTATCTTTGAAGTATCTAGCCGTTCTGTCTCTGGAGCAAAACCATTTAACGACATTGCCATCTTCACTGGGCTTGTTGACATCACTGAGGCAACTTAATGTTGCAAAAAACAAATTATCTAGCCTCCCAAATGAAATAGGACTTCTGACTAAGCTTGAAGTTCTGAAAGTGAACAATAACAGGATAAGCATCATTCCTACATGTATAGGGGATTGCAATGCTCTTATTGAGGTTGATGTTTCGTCAAATCTTCTTTCCGAGTTGCCAGAGACAATGGGAAATTTGAACAATCTGAAGTCTTTGTATCTCAGCAACAACGGTTTGAAAGCGCTTCCCACTGTGTTATTCAAAATGTGCTTACAGCTCACAACACTTGATCTGCACAATACAGAAATAACTGTGGATGTCCTTCGTCAGTTTGAAGGCTGGGAAAGCTTTGATGAACGACGCCGATTGAAGCATCAGAAGCAACTGGATTTCCGAGTTGTAAACTCCGGTGCATTTGATGAAGGTGCTGATAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_4 LRR_8 ubiquitin

Protein Analysis

301

Amino Acids

33.23

Weight (kDa)

6.46

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 118 - 203 1.8e-09 Leucine-rich repeat region
LRR_8 PF13855 153 - 210 5.6e-06 Leucine rich repeat
LRR_8 PF13855 176 - 233 4.3e-07 Leucine rich repeat
LRR_8 PF13855 222 - 267 5e-06 Leucine rich repeat
LRR_4 PF12799 222 - 260 6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 655
AcuI CTGAAG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 823
AfaI GTAC 2 cut(s) 238, 285
AfeI AGCGCT 1 cut(s) 705
AflIII ACRYGT 1 cut(s) 575
AgeI ACCGGT 1 cut(s) 150
AluBI AGCT 6 cut(s) 309, 363, 529, 739, 810, 900
AluI AGCT 6 cut(s) 309, 363, 529, 739, 810, 900
Alw26I GTCTC 2 cut(s) 408, 638
Aor51HI AGCGCT 1 cut(s) 705
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 655
AsiGI ACCGGT 1 cut(s) 150
AspLEI GCGC 2 cut(s) 73, 706
AspS9I GGNCC 1 cut(s) 53
AvaII GGWCC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 319
BccI CCATC 3 cut(s) 65, 156, 442
BceAI ACGGC 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 408, 638
BfaI CTAG 2 cut(s) 392, 495
BfoI RGCGCY 1 cut(s) 707
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 2 cut(s) 6, 55
BmrI ACTGGG 1 cut(s) 452
BmsI GCATC 3 cut(s) 6, 573, 844
BmuI ACTGGG 1 cut(s) 452
BpmI CTGGAG 2 cut(s) 195, 428
Bsa29I ATCGAT 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 1 cut(s) 823
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 2 cut(s) 150, 869
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 3 cut(s) 178, 447, 852
Bse3DI GCAATG 2 cut(s) 428, 598
Bse8I GATNNNNATC 1 cut(s) 563
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 3 cut(s) 57, 355, 784
BseJI GATNNNNATC 1 cut(s) 563
BseMI GCAATG 2 cut(s) 428, 598
BseMII CTCAG 4 cut(s) 44, 274, 452, 697
BseNI ACTGG 3 cut(s) 178, 447, 852
BseXI GCAGC 1 cut(s) 319
BseYI CCCAGC 2 cut(s) 363, 801
BsgI GTGCAG 1 cut(s) 740
BshTI ACCGGT 1 cut(s) 150
BshVI ATCGAT 1 cut(s) 14
BsiSI CCGG 2 cut(s) 151, 870
BslFI GGGAC 1 cut(s) 62
BsmAI GTCTC 2 cut(s) 408, 638
BsmFI GGGAC 1 cut(s) 62
Bsp143I GATC 2 cut(s) 11, 751
BspCNI CTCAG 4 cut(s) 43, 273, 453, 696
BspDI ATCGAT 1 cut(s) 14
BspLI GGNNCC 2 cut(s) 6, 55
BsrDI GCAATG 2 cut(s) 428, 598
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 3 cut(s) 178, 447, 852
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 2 cut(s) 11, 751
BssNI GRCGYC 1 cut(s) 823
BssT1I CCWWGG 1 cut(s) 43
Bst4CI ACNGT 4 cut(s) 189, 695, 715, 775
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 823
BstC8I GCNNGC 2 cut(s) 38, 330
BstDEI CTNAG 6 cut(s) 30, 260, 305, 461, 525, 683
BstF5I GGATG 3 cut(s) 57, 355, 784
BstH2I RGCGCY 1 cut(s) 707
BstHHI GCGC 2 cut(s) 73, 706
BstKTI GATC 2 cut(s) 14, 754
BstMAI GTCTC 2 cut(s) 408, 638
BstMBI GATC 2 cut(s) 11, 751
BstMWI GCNNNNNNNGC 2 cut(s) 807, 841
BstNSI RCATGY 1 cut(s) 579
BstV1I GCAGC 1 cut(s) 319
Bsu15I ATCGAT 1 cut(s) 14
BsuTUI ATCGAT 1 cut(s) 14
BtsCI GGATG 3 cut(s) 57, 355, 784
BtsIMutI CAGTG 3 cut(s) 440, 458, 711
Cac8I GCNNGC 2 cut(s) 38, 330
CfoI GCGC 2 cut(s) 73, 706
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 53
ClaI ATCGAT 1 cut(s) 14
CseI GACGC 1 cut(s) 831
Csp6I GTAC 2 cut(s) 237, 284
CspAI ACCGGT 1 cut(s) 150
CviAII CATG 2 cut(s) 22, 576
CviQI GTAC 2 cut(s) 237, 284
DdeI CTNAG 6 cut(s) 30, 260, 305, 461, 525, 683
DpnI GATC 2 cut(s) 13, 753
DpnII GATC 2 cut(s) 11, 751
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 53
Eco47III AGCGCT 1 cut(s) 705
Eco57I CTGAAG 1 cut(s) 689
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 2 cut(s) 25, 579
FaiI YATR 6 cut(s) 23, 182, 212, 344, 577, 581
FaqI GGGAC 1 cut(s) 62
FatI CATG 2 cut(s) 21, 575
Fnu4HI GCNGC 1 cut(s) 333
FokI GGATG 3 cut(s) 44, 362, 791
Fsp4HI GCNGC 1 cut(s) 333
FspBI CTAG 2 cut(s) 392, 495
GlaI GCGC 2 cut(s) 72, 705
GluI GCNGC 1 cut(s) 333
GsaI CCCAGC 2 cut(s) 367, 805
GsuI CTGGAG 2 cut(s) 195, 428
HaeII RGCGCY 1 cut(s) 707
HapII CCGG 2 cut(s) 151, 870
HgaI GACGC 1 cut(s) 831
HhaI GCGC 2 cut(s) 73, 706
Hin1I GRCGYC 1 cut(s) 823
Hin1II CATG 2 cut(s) 25, 579
Hin6I GCGC 2 cut(s) 71, 704
HinP1I GCGC 2 cut(s) 71, 704
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HindIII AAGCTT 2 cut(s) 527, 808
HinfI GANTC 2 cut(s) 93, 353
HpaII CCGG 2 cut(s) 151, 870
Hpy166II GTNNAC 3 cut(s) 453, 547, 865
Hpy188I TCNGA 8 cut(s) 98, 193, 522, 540, 634, 669, 840, 857
Hpy188III TCNNGA 3 cut(s) 90, 278, 407
Hpy8I GTNNAC 3 cut(s) 453, 547, 865
Hpy99I CGWCG 1 cut(s) 825
HpyAV CCTTC 7 cut(s) 160, 199, 297, 362, 791, 794, 878
HpyCH4III ACNGT 4 cut(s) 189, 695, 715, 775
HpyCH4V TGCA 8 cut(s) 40, 245, 316, 335, 479, 591, 757, 875
HpyF10VI GCNNNNNNNGC 2 cut(s) 807, 841
HpyF3I CTNAG 6 cut(s) 30, 260, 305, 461, 525, 683
Hsp92I GRCGYC 1 cut(s) 823
Hsp92II CATG 2 cut(s) 25, 579
HspAI GCGC 2 cut(s) 71, 704
Kzo9I GATC 2 cut(s) 11, 751
LmnI GCTCC 3 cut(s) 4, 314, 409
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 3 cut(s) 6, 573, 844
MaeI CTAG 2 cut(s) 392, 495
MalI GATC 2 cut(s) 13, 753
MboI GATC 2 cut(s) 11, 751
MboII GAAGA 3 cut(s) 129, 429, 617
MluCI AATT 5 cut(s) 99, 268, 320, 488, 655
MnlI CCTC 3 cut(s) 456, 509, 598
MseI TTAA 2 cut(s) 422, 471
MspI CCGG 2 cut(s) 151, 870
MwoI GCNNNNNNNGC 2 cut(s) 807, 841
NdeII GATC 2 cut(s) 11, 751
NlaIII CATG 2 cut(s) 25, 579
NlaIV GGNNCC 2 cut(s) 6, 55
NspI RCATGY 1 cut(s) 579
PciI ACATGT 1 cut(s) 575
PfeI GAWTC 2 cut(s) 93, 353
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 1 cut(s) 334
PscI ACATGT 1 cut(s) 575
PspFI CCCAGC 2 cut(s) 363, 801
PspN4I GGNNCC 2 cut(s) 6, 55
PspPI GGNCC 1 cut(s) 53
RsaI GTAC 2 cut(s) 238, 285
RsaNI GTAC 2 cut(s) 237, 284
SaqAI TTAA 2 cut(s) 422, 471
SatI GCNGC 1 cut(s) 333
Sau3AI GATC 2 cut(s) 11, 751
Sau96I GGNCC 1 cut(s) 53
SfaNI GCATC 3 cut(s) 6, 573, 844
SinI GGWCC 1 cut(s) 53
Sse9I AATT 5 cut(s) 99, 268, 320, 488, 655
SspMI CTAG 2 cut(s) 392, 495
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 4 cut(s) 189, 695, 715, 775
TaqI TCGA 2 cut(s) 14, 277
TasI AATT 5 cut(s) 99, 268, 320, 488, 655
TfiI GAWTC 2 cut(s) 93, 353
Tru1I TTAA 2 cut(s) 422, 471
Tru9I TTAA 2 cut(s) 422, 471
TscAI CASTG 3 cut(s) 447, 465, 718
TseI GCWGC 1 cut(s) 332
TspDTI ATGAA 4 cut(s) 234, 522, 831, 897
TspGWI ACGGA 2 cut(s) 66, 143
TspRI CASTG 3 cut(s) 447, 465, 718
VpaK11BI GGWCC 1 cut(s) 53
XapI RAATTY 1 cut(s) 655
XceI RCATGY 1 cut(s) 579
XspI CTAG 2 cut(s) 392, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.