RchiOBHm_Chr5g0070821

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009438
Physical Location & Seq
Forward (+)
76621079 .. 76622842
1764 bp
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UTR
Exon/CDS
Intron
PRQ34600

Sequence Viewer

Length: 636 bp
ATGAATTCCTTGCAGAAACTTTTACTCGATTCAAATGATATATCGGAGGACTCTGTTAGCTGGGAAGCACTAAAATCTTTAAAGTCTTTAACTGTTCTTTCTCTGAACCAAAACCGTTTAACTAGATTGTCATCGGATCTGGGCTGGTTGCCATCATTAAGGCAACTTCATGTTGCGAACAACATGTTGTCTGGCCTCCCAAGTGAATTGCGATATCTGCCTAAGCTTGAAGTTCTGAAAGTCAACAATAACAGGCCGGGTGATCTTGAAAGTATTCTAAGCAAGGATATGATCACCACCATTCCTCCATGGATAGGGGACCTTATTTCTCTTATTGAGGTTGATCTTTCATCAAATCTTCTGTCAGATTTGCCCGAGACATTTAGCAGTTTGCATAATCTAAAGTCCCTGTCTCTCAGTAAGAATGGGCTGAAATCTCTCCCTTCTTCGCTATTCAAAATGTGCCTAATGCTCACAAGACTTGACTTGCACAACACAGAAATAACCATGGATATCCTTCGTCAGATTGAGGGATGGGAAAACTTTGATGAACGCCGTCGCTTGAAGCAACGGAAGAAACTGGATTTTCGTGTTGTGGGCTCTGCTGCATTTGATGAAGGTGCTGATAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

211

Amino Acids

23.86

Weight (kDa)

6.32

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 3 - 63 6.1e-08 Leucine rich repeat
LRR_14 PF23598 15 - 112 8.9e-06 Leucine-rich repeat region
LRR_4 PF12799 28 - 66 3.7e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 51 - 91 2.8e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 104 - 144 1.5e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 144
AcsI RAATTY 1 cut(s) 4
AfiI CCNNNNNNNGG 1 cut(s) 314
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 5 cut(s) 33, 230, 269, 457, 565
AluBI AGCT 3 cut(s) 60, 226, 633
AluI AGCT 3 cut(s) 60, 226, 633
Alw26I GTCTC 2 cut(s) 371, 417
AlwI GGATC 1 cut(s) 144
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 2 cut(s) 193, 254
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 1 cut(s) 4
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
Asp700I GAANNNNTTC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 319
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 2 cut(s) 272, 286
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 602
BbvI GCAGC 1 cut(s) 592
BccI CCATC 2 cut(s) 160, 528
BceAI ACGGC 1 cut(s) 540
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 2 cut(s) 371, 417
BfaI CTAG 1 cut(s) 123
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 308, 507
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 585
Bse8I GATNNNNATC 1 cut(s) 130
BseDI CCNNGG 2 cut(s) 308, 507
BseGI GGATG 1 cut(s) 539
BseJI GATNNNNATC 1 cut(s) 130
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 430
BseNI ACTGG 1 cut(s) 585
BseXI GCAGC 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 195, 256
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 2 cut(s) 332, 391
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 2 cut(s) 371, 417
BsmFI GGGAC 2 cut(s) 332, 391
BsnI GGCC 2 cut(s) 195, 256
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 602
Bsp143I GATC 4 cut(s) 136, 262, 291, 343
Bsp19I CCATGG 2 cut(s) 308, 507
BspANI GGCC 2 cut(s) 195, 256
BspCNI CTCAG 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 320
BspPI GGATC 1 cut(s) 144
BsrI ACTGG 1 cut(s) 585
BssECI CCNNGG 2 cut(s) 308, 507
BssMI GATC 4 cut(s) 136, 262, 291, 343
BssT1I CCWWGG 2 cut(s) 308, 507
Bst4CI ACNGT 2 cut(s) 94, 116
BstDEI CTNAG 3 cut(s) 222, 278, 416
BstDSI CCRYGG 2 cut(s) 308, 507
BstF5I GGATG 1 cut(s) 539
BstKTI GATC 4 cut(s) 139, 265, 294, 346
BstMAI GTCTC 2 cut(s) 371, 417
BstMBI GATC 4 cut(s) 136, 262, 291, 343
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNSI RCATGY 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 592
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 2 cut(s) 195, 256
BtgI CCRYGG 2 cut(s) 308, 507
BtsCI GGATG 1 cut(s) 539
Cfr13I GGNCC 1 cut(s) 319
CviAII CATG 4 cut(s) 170, 184, 309, 508
CviJI RGCY 8 cut(s) 60, 144, 195, 226, 256, 430, 600, 633
CviKI_1 RGCY 8 cut(s) 60, 144, 195, 226, 256, 430, 600, 633
DdeI CTNAG 3 cut(s) 222, 278, 416
DpnI GATC 4 cut(s) 138, 264, 293, 345
DpnII GATC 4 cut(s) 136, 262, 291, 343
DraI TTTAAA 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 308, 507
Eco24I GRGCYC 1 cut(s) 602
Eco32I GATATC 2 cut(s) 215, 514
Eco47I GGWCC 1 cut(s) 319
Eco88I CYCGRG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 319
EcoRI GAATTC 1 cut(s) 4
EcoRV GATATC 2 cut(s) 215, 514
EcoT14I CCWWGG 2 cut(s) 308, 507
EcoT38I GRGCYC 1 cut(s) 602
ErhI CCWWGG 2 cut(s) 308, 507
FaeI CATG 4 cut(s) 173, 187, 312, 511
FaiI YATR 7 cut(s) 41, 171, 185, 290, 310, 396, 509
FaqI GGGAC 2 cut(s) 332, 391
FatI CATG 4 cut(s) 169, 183, 308, 507
FbaI TGATCA 1 cut(s) 291
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 1 cut(s) 546
FriOI GRGCYC 1 cut(s) 602
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 1 cut(s) 123
GluI GCNGC 1 cut(s) 606
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 2 cut(s) 195, 256
HapII CCGG 1 cut(s) 257
Hin1II CATG 4 cut(s) 173, 187, 312, 511
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 2 cut(s) 29, 50
HpaII CCGG 1 cut(s) 257
HphI GGTGA 2 cut(s) 272, 286
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 6 cut(s) 46, 105, 136, 237, 367, 525
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 1 cut(s) 561
HpyAV CCTTC 3 cut(s) 453, 527, 611
HpyCH4III ACNGT 2 cut(s) 94, 116
HpyCH4V TGCA 4 cut(s) 13, 394, 490, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 3 cut(s) 222, 278, 416
Hsp92II CATG 4 cut(s) 173, 187, 312, 511
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 4 cut(s) 136, 262, 291, 343
LpnPI CCDG 8 cut(s) 46, 125, 130, 177, 238, 270, 422, 566
Lsp1109I GCAGC 1 cut(s) 592
MaeI CTAG 1 cut(s) 123
MalI GATC 4 cut(s) 138, 264, 293, 345
MboI GATC 4 cut(s) 136, 262, 291, 343
MboII GAAGA 3 cut(s) 350, 438, 586
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 602
MluCI AATT 2 cut(s) 4, 206
MlyI GAGTC 1 cut(s) 44
MnlI CCTC 5 cut(s) 40, 206, 315, 331, 523
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 4 cut(s) 80, 89, 119, 158
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 258
MwoI GCNNNNNNNGC 1 cut(s) 217
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 2 cut(s) 308, 507
NdeII GATC 4 cut(s) 136, 262, 291, 343
NlaIII CATG 4 cut(s) 173, 187, 312, 511
NlaIV GGNNCC 1 cut(s) 320
NspI RCATGY 1 cut(s) 187
PciI ACATGT 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PpuMI RGGWCCY 1 cut(s) 319
PscI ACATGT 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 320
PspPI GGNCC 1 cut(s) 319
PspPPI RGGWCCY 1 cut(s) 319
PsuI RGATCY 1 cut(s) 136
SaqAI TTAA 4 cut(s) 80, 89, 119, 158
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 4 cut(s) 136, 262, 291, 343
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 602
SetI ASST 6 cut(s) 62, 228, 324, 342, 622, 635
SinI GGWCC 1 cut(s) 319
Sse9I AATT 2 cut(s) 4, 206
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 2 cut(s) 308, 507
TaaI ACNGT 2 cut(s) 94, 116
TaqI TCGA 1 cut(s) 27
TasI AATT 2 cut(s) 4, 206
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 80, 89, 119, 158
Tru9I TTAA 4 cut(s) 80, 89, 119, 158
TseI GCWGC 1 cut(s) 605
TspDTI ATGAA 5 cut(s) 17, 158, 339, 564, 630
TspGWI ACGGA 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.