Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009438

Physical Location & Seq

Genomic Coordinates Reverse (-)

5002750 .. 5006399

Gene Structure 3650 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1062200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGCAAGCAGTGCCACCGGCGGTGACGATAGCGGAGGCGCCACCGTAAGCCTCACCGTGAAGTTCAGCGGCAGAACAATTCCCATCACAAACCTCTCTGTGGACTCCACCATCGAAGACCTCAAGGCCCTTCTGCAGCCTCTCACCAATGTCCTCCCTCGCGGCCAAAAGCTCATCTGCAAAGGGAAGCTTCTGGTGGATGGAATGACACTGAAGGAGTCTGAGGTCAGGGATGGAGCCAAGATCATGCTCATGGCTTCTCAGGGCTTGCACCAAGGGGACGGACCCATCCTAAAACAAGCTCAGACACGCCCCATTTCCAGATTCAGAATTGTTGATACATTGGGAAAAGAAAAGACGGAAGTTCCTGTTGATAAGAACCGGTTGGATAGATGGAAGGCTACTGGAGTTATAGGACTGTCTGACTACAACTTGAAGGCTATACCTGATGAAGTGTGGGCTTCTGGGACTTCTGCAAGAGTTATTGAACTCAGCAATAATTCTATTCGAGATGTACCTTCAAAGATTGGTTGTCTAAACTCCTTGCAGAAATTGTTGCTCGCTGCAAATGATATAGTGGATGATTCTATTAGCTGGGAGGGAATAGTATCTTTGAAGTATCTAACTGTTCTGTCTCTGGAGCAAAACCATTTAACAATGTTGCCATCTTCACTGGGCTTGTTGACATCACTGAGGCAACTTAATGTTGCAAAAAACAAAGTATCTAGCCTCCCAAATGAAATAGGACTTCTGACTAAACTTGAAGTTCTGAAAGCGAACAATAACAGGATAAGCATCATCCCTACATGTATAGGGGATTGCAATGCTCTTATTGAGGTTGATGTTTCATCAAATCTTCTTTCCGAGTTGCCAGAGACAATGGGAAATTTGAACAATCTGAAGTCTTTGTATCTCAGCAACAACGGGTTGAAAGCACTTCCCTCTGCGTTATTCAAAATGTGCCTACAGCTAACAACACTTGATCTGCACAATACAGAAATAACTGTGGATGTCCTTCGTCAGTTTGAAGGCTGGGAAAGCTTTGATGAACGACGCCGGTTGAAGCATCAGAAGCAACTGGATTTCCGAGTTGTAAACTCCGGTGCATTTGATGAAGGTGCTGATAAGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04014 map04014

Pfam Domains

Protein Families

LRR_4 LRR_8 ubiquitin

Protein Analysis

378

Amino Acids

41.14

Weight (kDa)

6.91

Isoelectric Point (pI)

32.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ubiquitin	PF00240	18 - 88	5e-14	Ubiquitin family
LRR_14	PF23598	196 - 279	3.4e-09	Leucine-rich repeat region
LRR_8	PF13855	299 - 344	5.7e-06	Leucine rich repeat
LRR_4	PF12799	299 - 337	3.8e-06	Leucine Rich repeats (2 copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30105
fragaria_vesca	FvH4_3g39360 FvH4_3g39360 FvH4_3g39360
malus_domestica	MD03G1062200.v1.1
prunus_persica	Prupe.6G052000_v2.0.a1 Prupe.6G052000_v2.0.a1
pyrus_communis	pycom03g04960
rosa_chinensis	RchiOBHm_Chr5g0070801 RchiOBHm_Chr5g0070821
rosa_laevigata	RLG00000036159
rosa_multiflora	Rmu_sc0002944.1_g000022 Rmu_sc0006500.1_g000001 Rmu_sc0010241.1_g000005
rosa_roxburghii	Rroxscaffold_1G00010350
rosa_rugosa	Rorug05G0408500
rosa_samantha	Rh5AG464000 Rh5BG481800 Rh5CG506300 Rh5CG506500 Rh5DG494700
rosa_wichuraiana	Rw5G043190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	38
AccII	CGCG	1 cut(s)	162
AciI	CCGC	4 cut(s)	21, 33, 69, 162
AcoI	YGGCCR	1 cut(s)	163
AcsI	RAATTY	1 cut(s)	886
AcuI	CTGAAG	2 cut(s)	233, 920
AcyI	GRCGYC	2 cut(s)	39, 1054
AfaI	GTAC	1 cut(s)	516
AfiI	CCNNNNNNNGG	1 cut(s)	100
AflIII	ACRYGT	1 cut(s)	806
AgeI	ACCGGT	1 cut(s)	381
AluBI	AGCT	7 cut(s)	172, 190, 302, 594, 970, 1041, 1131
AluI	AGCT	7 cut(s)	172, 190, 302, 594, 970, 1041, 1131
Alw26I	GTCTC	2 cut(s)	639, 869
AoxI	GGCC	2 cut(s)	126, 163
ApeKI	GCWGC	2 cut(s)	136, 563
ApoI	RAATTY	1 cut(s)	886
AsiGI	ACCGGT	1 cut(s)	381
AspLEI	GCGC	1 cut(s)	41
AspS9I	GGNCC	2 cut(s)	127, 284
AsuHPI	GGTGA	3 cut(s)	35, 46, 136
AvaII	GGWCC	1 cut(s)	284
BanI	GGYRCC	1 cut(s)	38
BbsI	GAAGAC	1 cut(s)	123
BbvI	GCAGC	2 cut(s)	148, 550
BccI	CCATC	7 cut(s)	92, 119, 194, 227, 296, 387, 673
BcoDI	GTCTC	2 cut(s)	639, 869
BfaI	CTAG	1 cut(s)	726
BfmI	CTRYAG	2 cut(s)	134, 965
BfoI	RGCGCY	1 cut(s)	42
BisI	GCNGC	4 cut(s)	70, 137, 163, 564
BlsI	GCNGC	4 cut(s)	71, 138, 164, 565
Bme18I	GGWCC	1 cut(s)	284
BmgT120I	GGNCC	2 cut(s)	127, 284
BmiI	GGNNCC	3 cut(s)	40, 238, 286
BmrI	ACTGGG	1 cut(s)	683
BmsI	GCATC	2 cut(s)	804, 1075
BmuI	ACTGGG	1 cut(s)	683
BpiI	GAAGAC	1 cut(s)	123
BpmI	CTGGAG	2 cut(s)	426, 659
BpuEI	CTTGAG	1 cut(s)	107
BsaBI	GATNNNNATC	1 cut(s)	794
BsaHI	GRCGYC	2 cut(s)	39, 1054
BsaJI	CCNNGG	1 cut(s)	274
BsaWI	WCCGGW	2 cut(s)	381, 1100
Bsc4I	CCNNNNNNNGG	1 cut(s)	100
Bse118I	RCCGGY	3 cut(s)	17, 381, 1056
Bse1I	ACTGG	3 cut(s)	409, 678, 1083
Bse3DI	GCAATG	1 cut(s)	829
Bse8I	GATNNNNATC	1 cut(s)	794
BseDI	CCNNGG	1 cut(s)	274
BseGI	GGATG	6 cut(s)	205, 238, 288, 586, 798, 1015
BseJI	GATNNNNATC	1 cut(s)	794
BseLI	CCNNNNNNNGG	1 cut(s)	100
BseMI	GCAATG	1 cut(s)	829
BseMII	CTCAG	6 cut(s)	213, 275, 317, 505, 683, 928
BseNI	ACTGG	3 cut(s)	409, 678, 1083
BseXI	GCAGC	2 cut(s)	148, 550
BseYI	CCCAGC	2 cut(s)	594, 1032
BsgI	GTGCAG	1 cut(s)	971
Bsh1236I	CGCG	1 cut(s)	162
BshFI	GGCC	2 cut(s)	128, 165
BshNI	GGYRCC	1 cut(s)	38
BshTI	ACCGGT	1 cut(s)	381
BsiSI	CCGG	4 cut(s)	18, 382, 1057, 1101
BslFI	GGGAC	2 cut(s)	293, 481
BslI	CCNNNNNNNGG	1 cut(s)	100
BsmAI	GTCTC	2 cut(s)	639, 869
BsmFI	GGGAC	2 cut(s)	293, 481
BsnI	GGCC	2 cut(s)	128, 165
Bsp143I	GATC	2 cut(s)	243, 982
BspACI	CCGC	4 cut(s)	21, 33, 69, 162
BspANI	GGCC	2 cut(s)	128, 165
BspCNI	CTCAG	6 cut(s)	214, 274, 316, 504, 684, 927
BspFNI	CGCG	1 cut(s)	162
BspLI	GGNNCC	3 cut(s)	40, 238, 286
BspMAI	CTGCAG	1 cut(s)	138
BspT107I	GGYRCC	1 cut(s)	38
BsrDI	GCAATG	1 cut(s)	829
BsrFI	RCCGGY	3 cut(s)	17, 381, 1056
BsrI	ACTGG	3 cut(s)	409, 678, 1083
BssAI	RCCGGY	3 cut(s)	17, 381, 1056
BssECI	CCNNGG	1 cut(s)	274
BssMI	GATC	2 cut(s)	243, 982
BssNI	GRCGYC	2 cut(s)	39, 1054
BssT1I	CCWWGG	1 cut(s)	274
Bst4CI	ACNGT	5 cut(s)	46, 58, 420, 628, 1006
BstACI	GRCGYC	2 cut(s)	39, 1054
BstAPI	GCANNNNNTGC	1 cut(s)	11
BstC8I	GCNNGC	3 cut(s)	7, 269, 561
BstDEI	CTNAG	6 cut(s)	222, 261, 303, 491, 692, 914
BstF5I	GGATG	6 cut(s)	205, 238, 288, 586, 798, 1015
BstFNI	CGCG	1 cut(s)	162
BstH2I	RGCGCY	1 cut(s)	42
BstHHI	GCGC	1 cut(s)	41
BstKTI	GATC	2 cut(s)	246, 985
BstMAI	GTCTC	2 cut(s)	639, 869
BstMBI	GATC	2 cut(s)	243, 982
BstMWI	GCNNNNNNNGC	3 cut(s)	11, 1038, 1072
BstNSI	RCATGY	1 cut(s)	810
BstSFI	CTRYAG	2 cut(s)	134, 965
BstUI	CGCG	1 cut(s)	162
BstV1I	GCAGC	2 cut(s)	148, 550
BstV2I	GAAGAC	1 cut(s)	123
BsuRI	GGCC	2 cut(s)	128, 165
BtsCI	GGATG	6 cut(s)	205, 238, 288, 586, 798, 1015
BtsI	GCAGTG	1 cut(s)	16
BtsIMutI	CAGTG	4 cut(s)	16, 209, 671, 689
Cac8I	GCNNGC	3 cut(s)	7, 269, 561
CfoI	GCGC	1 cut(s)	41
Cfr10I	RCCGGY	3 cut(s)	17, 381, 1056
Cfr13I	GGNCC	2 cut(s)	127, 284
CseI	GACGC	1 cut(s)	1062
Csp6I	GTAC	1 cut(s)	515
CspAI	ACCGGT	1 cut(s)	381
CviAII	CATG	3 cut(s)	247, 253, 807
CviQI	GTAC	1 cut(s)	515
DdeI	CTNAG	6 cut(s)	222, 261, 303, 491, 692, 914
DinI	GGCGCC	1 cut(s)	40
DpnI	GATC	2 cut(s)	245, 984
DpnII	GATC	2 cut(s)	243, 982
EaeI	YGGCCR	1 cut(s)	163
Eco130I	CCWWGG	1 cut(s)	274
Eco47I	GGWCC	1 cut(s)	284
Eco57I	CTGAAG	2 cut(s)	233, 920
EcoO109I	RGGNCCY	1 cut(s)	127
EcoT14I	CCWWGG	1 cut(s)	274
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
ErhI	CCWWGG	1 cut(s)	274
FaeI	CATG	3 cut(s)	250, 256, 810
FaiI	YATR	7 cut(s)	248, 254, 413, 443, 575, 808, 812
FalI	AAGNNNNNCTT	2 cut(s)	174, 206
FaqI	GGGAC	2 cut(s)	293, 481
FatI	CATG	3 cut(s)	246, 252, 806
Fnu4HI	GCNGC	4 cut(s)	70, 137, 163, 564
FokI	GGATG	6 cut(s)	212, 245, 275, 593, 785, 1022
Fsp4HI	GCNGC	4 cut(s)	70, 137, 163, 564
FspBI	CTAG	1 cut(s)	726
GlaI	GCGC	1 cut(s)	40
GluI	GCNGC	4 cut(s)	70, 137, 163, 564
GsaI	CCCAGC	2 cut(s)	598, 1036
GsuI	CTGGAG	2 cut(s)	426, 659
HaeII	RGCGCY	1 cut(s)	42
HaeIII	GGCC	2 cut(s)	128, 165
HapII	CCGG	4 cut(s)	18, 382, 1057, 1101
HgaI	GACGC	1 cut(s)	1062
HhaI	GCGC	1 cut(s)	41
Hin1I	GRCGYC	2 cut(s)	39, 1054
Hin1II	CATG	3 cut(s)	250, 256, 810
Hin6I	GCGC	1 cut(s)	39
HinP1I	GCGC	1 cut(s)	39
HincII	GTYRAC	1 cut(s)	684
HindII	GTYRAC	1 cut(s)	684
HindIII	AAGCTT	2 cut(s)	188, 1039
HinfI	GANTC	4 cut(s)	104, 218, 324, 584
HpaII	CCGG	4 cut(s)	18, 382, 1057, 1101
HphI	GGTGA	3 cut(s)	35, 46, 136
Hpy166II	GTNNAC	3 cut(s)	103, 684, 1096
Hpy188III	TCNNGA	3 cut(s)	321, 509, 638
Hpy8I	GTNNAC	3 cut(s)	103, 684, 1096
Hpy99I	CGWCG	1 cut(s)	1056
HpyAV	CCTTC	8 cut(s)	140, 208, 391, 430, 528, 1022, 1025, 1109
HpyCH4III	ACNGT	5 cut(s)	46, 58, 420, 628, 1006
HpyF10VI	GCNNNNNNNGC	3 cut(s)	11, 1038, 1072
HpyF3I	CTNAG	6 cut(s)	222, 261, 303, 491, 692, 914
Hsp92I	GRCGYC	2 cut(s)	39, 1054
Hsp92II	CATG	3 cut(s)	250, 256, 810
HspAI	GCGC	1 cut(s)	39
KasI	GGCGCC	1 cut(s)	38
Kzo9I	GATC	2 cut(s)	243, 982
LmnI	GCTCC	2 cut(s)	236, 640
Lsp1109I	GCAGC	2 cut(s)	148, 550
LweI	GCATC	2 cut(s)	804, 1075
MaeI	CTAG	1 cut(s)	726
MaeIII	GTNAC	1 cut(s)	23
MalI	GATC	2 cut(s)	245, 984
MboI	GATC	2 cut(s)	243, 982
MboII	GAAGA	3 cut(s)	128, 660, 848
MluCI	AATT	5 cut(s)	78, 330, 499, 551, 886
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	2 cut(s)	98, 227
MmeI	TCCRAC	1 cut(s)	366
MseI	TTAA	2 cut(s)	653, 702
MslI	CAYNNNNRTG	1 cut(s)	251
MspA1I	CMGCKG	1 cut(s)	69
MspI	CCGG	4 cut(s)	18, 382, 1057, 1101
MvnI	CGCG	1 cut(s)	162
MwoI	GCNNNNNNNGC	3 cut(s)	11, 1038, 1072
NarI	GGCGCC	1 cut(s)	39
NdeII	GATC	2 cut(s)	243, 982
NlaIII	CATG	3 cut(s)	250, 256, 810
NlaIV	GGNNCC	3 cut(s)	40, 238, 286
NmuCI	GTSAC	1 cut(s)	23
NspI	RCATGY	1 cut(s)	810
PciI	ACATGT	1 cut(s)	806
PfeI	GAWTC	2 cut(s)	324, 584
PinAI	ACCGGT	1 cut(s)	381
PkrI	GCNGC	4 cut(s)	71, 138, 164, 565
PleI	GAGTC	2 cut(s)	98, 226
PluTI	GGCGCC	1 cut(s)	42
PpsI	GAGTC	2 cut(s)	98, 226
PscI	ACATGT	1 cut(s)	806
PspFI	CCCAGC	2 cut(s)	594, 1032
PspN4I	GGNNCC	3 cut(s)	40, 238, 286
PspPI	GGNCC	2 cut(s)	127, 284
PstI	CTGCAG	1 cut(s)	138
RsaI	GTAC	1 cut(s)	516
RsaNI	GTAC	1 cut(s)	515
RseI	CAYNNNNRTG	1 cut(s)	251
SaqAI	TTAA	2 cut(s)	653, 702
SatI	GCNGC	4 cut(s)	70, 137, 163, 564
Sau3AI	GATC	2 cut(s)	243, 982
Sau96I	GGNCC	2 cut(s)	127, 284
SchI	GAGTC	2 cut(s)	98, 227
SfaNI	GCATC	2 cut(s)	804, 1075
SfcI	CTRYAG	2 cut(s)	134, 965
SfoI	GGCGCC	1 cut(s)	40
SgrAI	CRCCGGYG	1 cut(s)	17
SinI	GGWCC	1 cut(s)	284
SmiMI	CAYNNNNRTG	1 cut(s)	251
SmlI	CTYRAG	1 cut(s)	122
SmoI	CTYRAG	1 cut(s)	122
Sse9I	AATT	5 cut(s)	78, 330, 499, 551, 886
SsiI	CCGC	4 cut(s)	21, 33, 69, 162
SspDI	GGCGCC	1 cut(s)	38
SspMI	CTAG	1 cut(s)	726
StyI	CCWWGG	1 cut(s)	274
TaaI	ACNGT	5 cut(s)	46, 58, 420, 628, 1006
TaqI	TCGA	2 cut(s)	114, 508
TasI	AATT	5 cut(s)	78, 330, 499, 551, 886
TauI	GCSGC	2 cut(s)	72, 165
TfiI	GAWTC	2 cut(s)	324, 584
Tru1I	TTAA	2 cut(s)	653, 702
Tru9I	TTAA	2 cut(s)	653, 702
TscAI	CASTG	4 cut(s)	16, 216, 678, 696
TseFI	GTSAC	1 cut(s)	23
TseI	GCWGC	2 cut(s)	136, 563
Tsp45I	GTSAC	1 cut(s)	23
TspDTI	ATGAA	5 cut(s)	465, 753, 837, 1062, 1128
TspGWI	ACGGA	2 cut(s)	297, 374
TspRI	CASTG	4 cut(s)	16, 216, 678, 696
VpaK11BI	GGWCC	1 cut(s)	284
XapI	RAATTY	1 cut(s)	886
XceI	RCATGY	1 cut(s)	810
XspI	CTAG	1 cut(s)	726

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.