RLG00000036159

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009438
Physical Location & Seq
Forward (+)
76668556 .. 76671868
3313 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036159

Sequence Viewer

Length: 1155 bp
ATGGAGACCGATGTCACCGGCGCTGACGACAGCGGAGCCGCCACCGCCACCGCCACTGCCATAAGCCTGAGCGTCAAGTTCAGTGGCCGAACCATACCCATCACAAATCTCTCACCGGACTCGACCATCAAGGACCTCAAGGCCCTTCTTCAACCTCTCACCAATGTCCTCCCTCGCGGCCAAAAGCTCATATCCAAGGGGAAGCTTTTGGTGGATGCAATGACGCTGAGGGAGTCTGAGATCACTAATGGGGCCAAGATCATGCTCATGGCTTCTCAGGGTTTGCACCAAGGGGATGGACCTATTCTTAAACAAGCTCAGAGTCGCCCCATTTCTAGAGACAACAATGCTGATAGATCCGATAAAGGAAAGGCGAAAAAAGCTGCTGTTGATAAGAATAGGTTGGAACGATGGAAGGCTACTGGAGTTATAGGACTCAGTGAGTGTAACTTAAAGGCTATTCCCGAGGAAGTCTGGGGGGATTCTAGAACTTCTGCAAGAGTTCTTGACCTCAGCAACAATGTTATTCAAGAAGTACCCGCCTTGATTGGCTGTATGAATTCCTTGCAGAAACTTTTACTCGATTCAAACGATATATCGGATGACTCTGTTAGCTGGGAAGCACTAAAATCTTTAAAGTCTTTAACTGTTCTTTCTCTGAACCAAAACCGTTTAACCAGATTGTCATCGGATCTGGGCTGGTTGCCATCATTAAGGCAACTTCATGTTGCGAACAACATGTTGTCTAGCCTCCCAAGTGAATTGCGATATCTGCCTAAGCTTGAAGTTCTGAAAGTCAACAATAACAGGATCACCACCATTCCTCCATGGATAGGGGACCTTATTTCTCTTCTTGAGGTTGATCTTTCATCAAATCTTCTGTCAGATTTGCCTGAGACATTTAGCAATTTGCATAATCTAAAGTCCCTGTCTCTCAGTAATAATGGGCTGAAATCTCTCCCTTCTTCGCTATTCAAAATGTGCCTAATGCTCACAAGACTTGACTTGCACAACACAGAAATAACCATGGATATCCTTCGTCAGATTGAGGGATGGGAAAACTTTGATGAACGTCGTCGTTTGAAGCAACAGAAGAAACTGGATTTTCGTGTTGTGGGCTCTGCTGCATTTGATGAAGGTGCTGATAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

385

Amino Acids

42.26

Weight (kDa)

8.63

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 24 - 93 1e-14 Ubiquitin family
LRR_8 PF13855 145 - 199 9.4e-06 Leucine rich repeat
LRR_14 PF23598 145 - 243 3.9e-06 Leucine-rich repeat region
LRR_14 PF23598 202 - 287 6.7e-08 Leucine-rich repeat region
LRR_8 PF13855 203 - 248 1.3e-06 Leucine rich repeat
LRR_4 PF12799 213 - 253 2.3e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 236 - 294 2.3e-07 Leucine rich repeat
LRR_8 PF13855 259 - 317 7.1e-10 Leucine rich repeat
LRR_4 PF12799 259 - 298 4.6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 177
AciI CCGC 6 cut(s) 33, 39, 45, 51, 177, 540
AclWI GGATC 3 cut(s) 351, 699, 818
AcoI YGGCCR 2 cut(s) 85, 178
AcsI RAATTY 1 cut(s) 559
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 833
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 6 cut(s) 152, 530, 588, 785, 976, 1084
AluBI AGCT 7 cut(s) 187, 205, 317, 383, 615, 781, 1152
AluI AGCT 7 cut(s) 187, 205, 317, 383, 615, 781, 1152
Alw26I GTCTC 3 cut(s) 333, 890, 936
AlwI GGATC 3 cut(s) 351, 699, 818
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 4 cut(s) 85, 141, 178, 252
ApeKI GCWGC 2 cut(s) 383, 1124
ApoI RAATTY 1 cut(s) 559
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 5 cut(s) 133, 142, 252, 299, 838
AsuHPI GGTGA 4 cut(s) 7, 105, 151, 805
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 3 cut(s) 133, 299, 838
BanII GRGCYC 1 cut(s) 1121
BbvCI CCTCAGC 2 cut(s) 227, 512
BbvI GCAGC 2 cut(s) 370, 1111
BccI CCATC 6 cut(s) 107, 134, 290, 405, 715, 1047
BcoDI GTCTC 3 cut(s) 333, 890, 936
BfaI CTAG 3 cut(s) 336, 486, 747
BfoI RGCGCY 1 cut(s) 24
BisI GCNGC 4 cut(s) 39, 178, 384, 1125
BlsI GCNGC 4 cut(s) 40, 179, 385, 1126
Bme18I GGWCC 3 cut(s) 133, 299, 838
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 5 cut(s) 133, 142, 252, 299, 838
BmiI GGNNCC 3 cut(s) 37, 253, 839
BmsI GCATC 1 cut(s) 205
BoxI GACNNNNGTC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 4 cut(s) 68, 227, 512, 777
BpuEI CTTGAG 2 cut(s) 122, 875
BsaBI GATNNNNATC 1 cut(s) 685
BsaJI CCNNGG 5 cut(s) 195, 289, 465, 827, 1026
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 808, 838
Bsc4I CCNNNNNNNGG 1 cut(s) 833
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 2 cut(s) 427, 1104
Bse3DI GCAATG 1 cut(s) 225
Bse8I GATNNNNATC 1 cut(s) 685
BseDI CCNNGG 5 cut(s) 195, 289, 465, 827, 1026
BseGI GGATG 4 cut(s) 220, 301, 607, 1058
BseJI GATNNNNATC 1 cut(s) 685
BseLI CCNNNNNNNGG 1 cut(s) 833
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 9 cut(s) 59, 218, 228, 290, 332, 451, 526, 885, 949
BseNI ACTGG 2 cut(s) 427, 1104
BseXI GCAGC 2 cut(s) 370, 1111
BseYI CCCAGC 1 cut(s) 615
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 4 cut(s) 87, 143, 180, 254
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 2 cut(s) 18, 116
BslFI GGGAC 2 cut(s) 851, 910
BslI CCNNNNNNNGG 1 cut(s) 833
BsmAI GTCTC 3 cut(s) 333, 890, 936
BsmFI GGGAC 2 cut(s) 851, 910
BsnI GGCC 4 cut(s) 87, 143, 180, 254
BsoBI CYCGRG 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 1121
Bsp143I GATC 6 cut(s) 240, 258, 356, 691, 810, 862
Bsp19I CCATGG 2 cut(s) 827, 1026
BspACI CCGC 6 cut(s) 33, 39, 45, 51, 177, 540
BspANI GGCC 4 cut(s) 87, 143, 180, 254
BspCNI CTCAG 9 cut(s) 60, 219, 229, 289, 331, 450, 525, 886, 948
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 3 cut(s) 37, 253, 839
BspPI GGATC 3 cut(s) 351, 699, 818
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 2 cut(s) 427, 1104
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 5 cut(s) 195, 289, 465, 827, 1026
BssMI GATC 6 cut(s) 240, 258, 356, 691, 810, 862
BssT1I CCWWGG 4 cut(s) 195, 289, 827, 1026
Bst4CI ACNGT 2 cut(s) 649, 671
Bst6I CTCTTC 1 cut(s) 855
BstDSI CCRYGG 2 cut(s) 827, 1026
BstF5I GGATG 4 cut(s) 220, 301, 607, 1058
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 6 cut(s) 243, 261, 359, 694, 813, 865
BstMAI GTCTC 3 cut(s) 333, 890, 936
BstMBI GATC 6 cut(s) 240, 258, 356, 691, 810, 862
BstMWI GCNNNNNNNGC 3 cut(s) 44, 380, 772
BstNSI RCATGY 1 cut(s) 742
BstPAI GACNNNNGTC 1 cut(s) 11
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 2 cut(s) 370, 1111
BstX2I RGATCY 2 cut(s) 356, 691
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 2 cut(s) 356, 691
BsuRI GGCC 4 cut(s) 87, 143, 180, 254
BtgI CCRYGG 2 cut(s) 827, 1026
BtsCI GGATG 4 cut(s) 220, 301, 607, 1058
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 3 cut(s) 54, 88, 445
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 5 cut(s) 133, 142, 252, 299, 838
CseI GACGC 2 cut(s) 61, 232
Csp6I GTAC 1 cut(s) 536
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviAII CATG 6 cut(s) 262, 268, 725, 739, 828, 1027
CviQI GTAC 1 cut(s) 536
DpnI GATC 6 cut(s) 242, 260, 358, 693, 812, 864
DpnII GATC 6 cut(s) 240, 258, 356, 691, 810, 862
DraI TTTAAA 1 cut(s) 636
EaeI YGGCCR 2 cut(s) 85, 178
Eam1104I CTCTTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 855
Eco130I CCWWGG 4 cut(s) 195, 289, 827, 1026
Eco24I GRGCYC 1 cut(s) 1121
Eco32I GATATC 2 cut(s) 770, 1033
Eco47I GGWCC 3 cut(s) 133, 299, 838
Eco88I CYCGRG 1 cut(s) 464
EcoO109I RGGNCCY 3 cut(s) 133, 142, 838
EcoRI GAATTC 1 cut(s) 559
EcoRV GATATC 2 cut(s) 770, 1033
EcoT14I CCWWGG 4 cut(s) 195, 289, 827, 1026
EcoT38I GRGCYC 1 cut(s) 1121
ErhI CCWWGG 4 cut(s) 195, 289, 827, 1026
FaeI CATG 6 cut(s) 265, 271, 728, 742, 831, 1030
FaqI GGGAC 2 cut(s) 851, 910
FatI CATG 6 cut(s) 261, 267, 724, 738, 827, 1026
FauI CCCGC 1 cut(s) 547
Fnu4HI GCNGC 4 cut(s) 39, 178, 384, 1125
FokI GGATG 4 cut(s) 227, 308, 614, 1065
FriOI GRGCYC 1 cut(s) 1121
Fsp4HI GCNGC 4 cut(s) 39, 178, 384, 1125
FspBI CTAG 3 cut(s) 336, 486, 747
GlaI GCGC 1 cut(s) 22
GluI GCNGC 4 cut(s) 39, 178, 384, 1125
GsaI CCCAGC 1 cut(s) 619
GsuI CTGGAG 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 4 cut(s) 87, 143, 180, 254
HapII CCGG 2 cut(s) 18, 116
HgaI GACGC 2 cut(s) 61, 232
HhaI GCGC 1 cut(s) 23
Hin1II CATG 6 cut(s) 265, 271, 728, 742, 831, 1030
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HindIII AAGCTT 2 cut(s) 203, 779
HinfI GANTC 7 cut(s) 119, 233, 322, 435, 482, 584, 605
HpaII CCGG 2 cut(s) 18, 116
HphI GGTGA 4 cut(s) 7, 105, 151, 805
Hpy166II GTNNAC 1 cut(s) 799
Hpy188I TCNGA 9 cut(s) 238, 321, 361, 601, 660, 691, 792, 886, 1044
Hpy188III TCNNGA 6 cut(s) 336, 464, 486, 506, 530, 854
Hpy8I GTNNAC 1 cut(s) 799
Hpy99I CGWCG 2 cut(s) 1077, 1080
HpyAV CCTTC 5 cut(s) 155, 409, 972, 1046, 1130
HpyCH4III ACNGT 2 cut(s) 649, 671
HpyCH4IV ACGT 1 cut(s) 1072
HpyCH4V TGCA 7 cut(s) 218, 286, 497, 568, 913, 1009, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 380, 772
HpySE526I ACGT 1 cut(s) 1072
Hsp92II CATG 6 cut(s) 265, 271, 728, 742, 831, 1030
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 6 cut(s) 240, 258, 356, 691, 810, 862
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 2 cut(s) 370, 1111
LweI GCATC 1 cut(s) 205
MaeI CTAG 3 cut(s) 336, 486, 747
MaeII ACGT 1 cut(s) 1072
MaeIII GTNAC 2 cut(s) 13, 446
MalI GATC 6 cut(s) 242, 260, 358, 693, 812, 864
MboI GATC 6 cut(s) 240, 258, 356, 691, 810, 862
MboII GAAGA 5 cut(s) 140, 842, 869, 957, 1105
MflI RGATCY 2 cut(s) 356, 691
MhlI GDGCHC 1 cut(s) 1121
MluCI AATT 3 cut(s) 559, 761, 907
MlyI GAGTC 5 cut(s) 113, 242, 331, 429, 599
MmeI TCCRAC 1 cut(s) 384
MseI TTAA 6 cut(s) 309, 452, 635, 644, 674, 713
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 2 cut(s) 18, 116
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 44, 380, 772
NcoI CCATGG 2 cut(s) 827, 1026
NdeII GATC 6 cut(s) 240, 258, 356, 691, 810, 862
NlaIII CATG 6 cut(s) 265, 271, 728, 742, 831, 1030
NlaIV GGNNCC 3 cut(s) 37, 253, 839
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 1 cut(s) 742
PciI ACATGT 1 cut(s) 738
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PfeI GAWTC 2 cut(s) 482, 584
PkrI GCNGC 4 cut(s) 40, 179, 385, 1126
PleI GAGTC 5 cut(s) 113, 241, 330, 429, 599
PpsI GAGTC 5 cut(s) 113, 241, 330, 429, 599
PpuMI RGGWCCY 2 cut(s) 133, 838
PscI ACATGT 1 cut(s) 738
PshAI GACNNNNGTC 1 cut(s) 11
Psp5II RGGWCCY 2 cut(s) 133, 838
PspFI CCCAGC 1 cut(s) 615
PspN4I GGNNCC 3 cut(s) 37, 253, 839
PspPI GGNCC 5 cut(s) 133, 142, 252, 299, 838
PspPPI RGGWCCY 2 cut(s) 133, 838
PsuI RGATCY 2 cut(s) 356, 691
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 6 cut(s) 309, 452, 635, 644, 674, 713
SatI GCNGC 4 cut(s) 39, 178, 384, 1125
Sau3AI GATC 6 cut(s) 240, 258, 356, 691, 810, 862
Sau96I GGNCC 5 cut(s) 133, 142, 252, 299, 838
SchI GAGTC 5 cut(s) 113, 242, 331, 429, 599
SduI GDGCHC 1 cut(s) 1121
SfaNI GCATC 1 cut(s) 205
SgrAI CRCCGGYG 1 cut(s) 17
SinI GGWCC 3 cut(s) 133, 299, 838
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 2 cut(s) 137, 854
SmoI CTYRAG 2 cut(s) 137, 854
Sse9I AATT 3 cut(s) 559, 761, 907
SsiI CCGC 6 cut(s) 33, 39, 45, 51, 177, 540
SspMI CTAG 3 cut(s) 336, 486, 747
StyI CCWWGG 4 cut(s) 195, 289, 827, 1026
TaaI ACNGT 2 cut(s) 649, 671
TaiI ACGT 1 cut(s) 1075
TaqI TCGA 2 cut(s) 122, 582
TaqII GACCGA 1 cut(s) 23
TasI AATT 3 cut(s) 559, 761, 907
TauI GCSGC 2 cut(s) 41, 180
TfiI GAWTC 2 cut(s) 482, 584
Tru1I TTAA 6 cut(s) 309, 452, 635, 644, 674, 713
Tru9I TTAA 6 cut(s) 309, 452, 635, 644, 674, 713
TscAI CASTG 3 cut(s) 61, 88, 445
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 2 cut(s) 383, 1124
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 5 cut(s) 572, 713, 858, 1083, 1149
TspRI CASTG 3 cut(s) 61, 88, 445
VpaK11BI GGWCC 3 cut(s) 133, 299, 838
XapI RAATTY 1 cut(s) 559
XbaI TCTAGA 2 cut(s) 335, 485
XceI RCATGY 1 cut(s) 742
XspI CTAG 3 cut(s) 336, 486, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.