Rroxscaffold_1G00010350

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009438
Physical Location & Seq
Reverse (-)
13090365 .. 13093976
3612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00010350.1

Sequence Viewer

Length: 1164 bp
ATGGAGACCGGTGTCTCCGGCGCTGACGTCAGCGGAACCGCCCCCACCGCCACCGCCACCGCCACCACCATAAGCCTGAGCGTCAAGTTCAGTGGCCGAACCATACCCATCACAAATCTCTCACCGGACTCGACCATCAAGGACCTCAAGGCCCTTCTTCAACCTCTCACCAACGTCCTCCCTCGCGGCCAAAAGCTCATATCCAAAGGGAAGCTTTTGGTGGATGCAATGACGCTGAGGGAGTCTGAGATCACTAATGGGTCCAAGATCATGCTCATGGCTTCTCAGGGTTTGCACCAAGGGGATGGACCTATTCTTAAACAAGCTCAGAGTCGCCCCATTTCTAGAGACAACAACGCTGATAGATCCGATAAAGGAAAGGCGAAAAAAGCTGCTGTTGATAAGAATAGGTTGGAACGATGGAGGGCTACTGGAGTTATAGGACTCAGTGAGTGTAACTTGAAGGCTATACCCGAGGAAGTCTGGGGGGATTCCAGAACTTCTGCAAGAGTTCTTGACCTCAGCAACAATGTTATTCAAGAAGTACCCGCCTTGATTGGCTGTATGAATTCCTTGCAGAAACTTTTACTCGATTCAAACGATATATCGGATGACTCTGTTAGCTGGGAAGCACTAAAATCTTTAAAGTCTTTAACTGTTCTTTCTCTGAACCAAAACCGTTTAACTAGATTGTCATCGGATCTGGGCTGGTTGCCATCACTAAGGCAACTTCATGTTGCGAACAACATGTTGTCTAGCCTCCCAAGTGAATTGCGATATCTGCCTAAGCTTGAAGTTCTGAAAGTCAACAATAACAGGATCACCACCATTCCTCCATGGATAGGGGACCTTATTTCTCTTATTGAGGTTGATCTTTCATCAAATCTTCTGTCAGATTTGCCCGAGACATTTAGCAATTTGCATAATCTAAAGTCCCTGTCTCTCAGTAATAATGGGCTGAAATCTCTCCCTTCTTCGCTATTCAAAATGTGCCTAATGCTCACAAGACTTGACTTGCACAACACAGAAATAACCATGGATATCCTTCGTCAGATTGAGGGATGGGAAAACTTTGATGAACGCCGTCGCTTGAAGCAACAGAAGAAACTGGATTTTCGTGTTGTGGGCTCTGCTGCATTTGATGAAGGTGCTGATAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

387

Amino Acids

42.54

Weight (kDa)

9.01

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 27 - 96 4.5e-15 Ubiquitin family
LRR_8 PF13855 148 - 202 9.5e-06 Leucine rich repeat
LRR_14 PF23598 148 - 247 3.7e-06 Leucine-rich repeat region
LRR_14 PF23598 205 - 290 5.7e-08 Leucine-rich repeat region
LRR_8 PF13855 206 - 251 1.2e-06 Leucine rich repeat
LRR_4 PF12799 216 - 256 2.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 239 - 297 2.6e-07 Leucine rich repeat
LRR_4 PF12799 239 - 280 6e-08 Leucine Rich repeats (2 copies)
LRR_8 PF13855 262 - 320 4e-10 Leucine rich repeat
LRR_4 PF12799 262 - 301 1.8e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
AccII CGCG 1 cut(s) 186
AciI CCGC 7 cut(s) 33, 39, 48, 54, 60, 186, 549
AclWI GGATC 3 cut(s) 360, 708, 827
AcoI YGGCCR 2 cut(s) 94, 187
AcsI RAATTY 1 cut(s) 568
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 1 cut(s) 546
AfiI CCNNNNNNNGG 1 cut(s) 842
AflIII ACRYGT 1 cut(s) 747
AgeI ACCGGT 1 cut(s) 8
AgsI TTSAA 7 cut(s) 161, 463, 539, 597, 794, 985, 1093
AluBI AGCT 7 cut(s) 196, 214, 326, 392, 624, 790, 1161
AluI AGCT 7 cut(s) 196, 214, 326, 392, 624, 790, 1161
Alw26I GTCTC 4 cut(s) 19, 342, 899, 945
AlwI GGATC 3 cut(s) 360, 708, 827
Ama87I CYCGRG 2 cut(s) 473, 902
AoxI GGCC 3 cut(s) 94, 150, 187
ApeKI GCWGC 2 cut(s) 392, 1133
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 2 cut(s) 875, 907
AsiGI ACCGGT 1 cut(s) 8
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 5 cut(s) 142, 151, 261, 308, 847
AsuHPI GGTGA 3 cut(s) 114, 160, 814
AvaI CYCGRG 2 cut(s) 473, 902
AvaII GGWCC 4 cut(s) 142, 261, 308, 847
BanII GRGCYC 1 cut(s) 1130
BbvCI CCTCAGC 2 cut(s) 236, 521
BbvI GCAGC 2 cut(s) 379, 1120
BccI CCATC 6 cut(s) 116, 143, 299, 414, 724, 1056
BceAI ACGGC 1 cut(s) 1068
BcoDI GTCTC 4 cut(s) 19, 342, 899, 945
BfaI CTAG 3 cut(s) 345, 687, 756
BfoI RGCGCY 1 cut(s) 24
BisI GCNGC 3 cut(s) 187, 393, 1134
BlsI GCNGC 3 cut(s) 188, 394, 1135
Bme18I GGWCC 4 cut(s) 142, 261, 308, 847
BmeT110I CYCGRG 2 cut(s) 473, 902
BmgT120I GGNCC 5 cut(s) 142, 151, 261, 308, 847
BmiI GGNNCC 3 cut(s) 37, 262, 848
BmsI GCATC 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 4 cut(s) 77, 236, 521, 786
BpuEI CTTGAG 1 cut(s) 131
BsaBI GATNNNNATC 1 cut(s) 694
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 4 cut(s) 298, 474, 836, 1035
BsaWI WCCGGW 2 cut(s) 8, 124
BsaXI ACNNNNNCTCC 2 cut(s) 817, 847
Bsc4I CCNNNNNNNGG 1 cut(s) 842
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 2 cut(s) 436, 1113
Bse3DI GCAATG 1 cut(s) 234
Bse8I GATNNNNATC 1 cut(s) 694
BseDI CCNNGG 4 cut(s) 298, 474, 836, 1035
BseGI GGATG 4 cut(s) 229, 310, 616, 1067
BseJI GATNNNNATC 1 cut(s) 694
BseLI CCNNNNNNNGG 1 cut(s) 842
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 8 cut(s) 68, 227, 237, 299, 341, 460, 535, 958
BseNI ACTGG 2 cut(s) 436, 1113
BseXI GCAGC 2 cut(s) 379, 1120
BseYI CCCAGC 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 96, 152, 189
BshTI ACCGGT 1 cut(s) 8
BsiHKCI CYCGRG 2 cut(s) 473, 902
BsiSI CCGG 3 cut(s) 9, 18, 125
BslFI GGGAC 2 cut(s) 860, 919
BslI CCNNNNNNNGG 1 cut(s) 842
BsmAI GTCTC 4 cut(s) 19, 342, 899, 945
BsmFI GGGAC 2 cut(s) 860, 919
BsnI GGCC 3 cut(s) 96, 152, 189
BsoBI CYCGRG 2 cut(s) 473, 902
Bsp1286I GDGCHC 1 cut(s) 1130
Bsp143I GATC 6 cut(s) 249, 267, 365, 700, 819, 871
Bsp19I CCATGG 2 cut(s) 836, 1035
BspACI CCGC 7 cut(s) 33, 39, 48, 54, 60, 186, 549
BspANI GGCC 3 cut(s) 96, 152, 189
BspCNI CTCAG 8 cut(s) 69, 228, 238, 298, 340, 459, 534, 957
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 3 cut(s) 37, 262, 848
BspPI GGATC 3 cut(s) 360, 708, 827
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 8
BsrI ACTGG 2 cut(s) 436, 1113
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 4 cut(s) 298, 474, 836, 1035
BssMI GATC 6 cut(s) 249, 267, 365, 700, 819, 871
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 3 cut(s) 298, 836, 1035
Bst4CI ACNGT 2 cut(s) 658, 680
BstACI GRCGYC 1 cut(s) 27
BstDSI CCRYGG 2 cut(s) 836, 1035
BstF5I GGATG 4 cut(s) 229, 310, 616, 1067
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 6 cut(s) 252, 270, 368, 703, 822, 874
BstMAI GTCTC 4 cut(s) 19, 342, 899, 945
BstMBI GATC 6 cut(s) 249, 267, 365, 700, 819, 871
BstMWI GCNNNNNNNGC 3 cut(s) 47, 389, 781
BstNSI RCATGY 1 cut(s) 751
BstPAI GACNNNNGTC 1 cut(s) 11
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 379, 1120
BstX2I RGATCY 2 cut(s) 365, 700
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 2 cut(s) 365, 700
BsuRI GGCC 3 cut(s) 96, 152, 189
BtgI CCRYGG 2 cut(s) 836, 1035
BtsCI GGATG 4 cut(s) 229, 310, 616, 1067
BtsIMutI CAGTG 2 cut(s) 97, 454
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 5 cut(s) 142, 151, 261, 308, 847
CseI GACGC 2 cut(s) 70, 241
Csp6I GTAC 1 cut(s) 545
CspAI ACCGGT 1 cut(s) 8
CspCI CAANNNNNGTGG 2 cut(s) 73, 108
CviAII CATG 6 cut(s) 271, 277, 734, 748, 837, 1036
CviQI GTAC 1 cut(s) 545
DpnI GATC 6 cut(s) 251, 269, 367, 702, 821, 873
DpnII GATC 6 cut(s) 249, 267, 365, 700, 819, 871
DraI TTTAAA 1 cut(s) 645
EaeI YGGCCR 2 cut(s) 94, 187
Eco130I CCWWGG 3 cut(s) 298, 836, 1035
Eco24I GRGCYC 1 cut(s) 1130
Eco32I GATATC 2 cut(s) 779, 1042
Eco47I GGWCC 4 cut(s) 142, 261, 308, 847
Eco88I CYCGRG 2 cut(s) 473, 902
EcoO109I RGGNCCY 3 cut(s) 142, 151, 847
EcoRI GAATTC 1 cut(s) 568
EcoRV GATATC 2 cut(s) 779, 1042
EcoT14I CCWWGG 3 cut(s) 298, 836, 1035
EcoT38I GRGCYC 1 cut(s) 1130
ErhI CCWWGG 3 cut(s) 298, 836, 1035
FaeI CATG 6 cut(s) 274, 280, 737, 751, 840, 1039
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 2 cut(s) 860, 919
FatI CATG 6 cut(s) 270, 276, 733, 747, 836, 1035
FauI CCCGC 1 cut(s) 556
Fnu4HI GCNGC 3 cut(s) 187, 393, 1134
FokI GGATG 4 cut(s) 236, 317, 623, 1074
FriOI GRGCYC 1 cut(s) 1130
Fsp4HI GCNGC 3 cut(s) 187, 393, 1134
FspBI CTAG 3 cut(s) 345, 687, 756
GlaI GCGC 1 cut(s) 22
GluI GCNGC 3 cut(s) 187, 393, 1134
GsaI CCCAGC 1 cut(s) 628
GsuI CTGGAG 1 cut(s) 453
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 3 cut(s) 96, 152, 189
HapII CCGG 3 cut(s) 9, 18, 125
HgaI GACGC 2 cut(s) 70, 241
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 6 cut(s) 274, 280, 737, 751, 840, 1039
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 2 cut(s) 212, 788
HinfI GANTC 7 cut(s) 128, 242, 331, 444, 491, 593, 614
HpaII CCGG 3 cut(s) 9, 18, 125
HphI GGTGA 3 cut(s) 114, 160, 814
Hpy166II GTNNAC 1 cut(s) 808
Hpy188I TCNGA 9 cut(s) 247, 330, 370, 610, 669, 700, 801, 895, 1053
Hpy188III TCNNGA 4 cut(s) 345, 495, 515, 539
Hpy8I GTNNAC 1 cut(s) 808
Hpy99I CGWCG 1 cut(s) 1089
HpyAV CCTTC 5 cut(s) 164, 457, 981, 1055, 1139
HpyCH4III ACNGT 2 cut(s) 658, 680
HpyCH4IV ACGT 2 cut(s) 27, 174
HpyCH4V TGCA 7 cut(s) 227, 295, 506, 577, 922, 1018, 1136
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 389, 781
HpySE526I ACGT 2 cut(s) 27, 174
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 6 cut(s) 274, 280, 737, 751, 840, 1039
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 6 cut(s) 249, 267, 365, 700, 819, 871
Lsp1109I GCAGC 2 cut(s) 379, 1120
LweI GCATC 1 cut(s) 214
MaeI CTAG 3 cut(s) 345, 687, 756
MaeII ACGT 2 cut(s) 27, 174
MaeIII GTNAC 1 cut(s) 455
MalI GATC 6 cut(s) 251, 269, 367, 702, 821, 873
MboI GATC 6 cut(s) 249, 267, 365, 700, 819, 871
MboII GAAGA 4 cut(s) 149, 878, 966, 1114
MflI RGATCY 2 cut(s) 365, 700
MhlI GDGCHC 1 cut(s) 1130
MluCI AATT 3 cut(s) 568, 770, 916
MlyI GAGTC 5 cut(s) 122, 251, 340, 438, 608
MmeI TCCRAC 1 cut(s) 393
MseI TTAA 4 cut(s) 318, 644, 653, 683
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 3 cut(s) 9, 18, 125
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 47, 389, 781
NcoI CCATGG 2 cut(s) 836, 1035
NdeII GATC 6 cut(s) 249, 267, 365, 700, 819, 871
NlaIII CATG 6 cut(s) 274, 280, 737, 751, 840, 1039
NlaIV GGNNCC 3 cut(s) 37, 262, 848
NspI RCATGY 1 cut(s) 751
PciI ACATGT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PfeI GAWTC 2 cut(s) 491, 593
PinAI ACCGGT 1 cut(s) 8
PkrI GCNGC 3 cut(s) 188, 394, 1135
PleI GAGTC 5 cut(s) 122, 250, 339, 438, 608
PpsI GAGTC 5 cut(s) 122, 250, 339, 438, 608
PpuMI RGGWCCY 2 cut(s) 142, 847
PscI ACATGT 1 cut(s) 747
PshAI GACNNNNGTC 1 cut(s) 11
Psp5II RGGWCCY 2 cut(s) 142, 847
PspFI CCCAGC 1 cut(s) 624
PspN4I GGNNCC 3 cut(s) 37, 262, 848
PspPI GGNCC 5 cut(s) 142, 151, 261, 308, 847
PspPPI RGGWCCY 2 cut(s) 142, 847
PsuI RGATCY 2 cut(s) 365, 700
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 4 cut(s) 318, 644, 653, 683
SatI GCNGC 3 cut(s) 187, 393, 1134
Sau3AI GATC 6 cut(s) 249, 267, 365, 700, 819, 871
Sau96I GGNCC 5 cut(s) 142, 151, 261, 308, 847
SchI GAGTC 5 cut(s) 122, 251, 340, 438, 608
SduI GDGCHC 1 cut(s) 1130
SfaNI GCATC 1 cut(s) 214
SinI GGWCC 4 cut(s) 142, 261, 308, 847
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 3 cut(s) 568, 770, 916
SsiI CCGC 7 cut(s) 33, 39, 48, 54, 60, 186, 549
SspMI CTAG 3 cut(s) 345, 687, 756
StyI CCWWGG 3 cut(s) 298, 836, 1035
TaaI ACNGT 2 cut(s) 658, 680
TaiI ACGT 2 cut(s) 30, 177
TaqI TCGA 2 cut(s) 131, 591
TasI AATT 3 cut(s) 568, 770, 916
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 2 cut(s) 491, 593
Tru1I TTAA 4 cut(s) 318, 644, 653, 683
Tru9I TTAA 4 cut(s) 318, 644, 653, 683
TscAI CASTG 2 cut(s) 97, 454
TseI GCWGC 2 cut(s) 392, 1133
TspDTI ATGAA 5 cut(s) 581, 722, 867, 1092, 1158
TspRI CASTG 2 cut(s) 97, 454
VpaK11BI GGWCC 4 cut(s) 142, 261, 308, 847
XapI RAATTY 1 cut(s) 568
XbaI TCTAGA 1 cut(s) 344
XceI RCATGY 1 cut(s) 751
XspI CTAG 3 cut(s) 345, 687, 756
ZraI GACGTC 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.