RchiOBHm_Chr5g0070801

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009438
Physical Location & Seq
Forward (+)
76604400 .. 76607946
3547 bp
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UTR
Exon/CDS
Intron
PRQ34599

Sequence Viewer

Length: 1152 bp
ATGGAGACCGGTGTCACCGGCGCTGACGTCAGCGGAGCGACCACCACCACCACCACCATAAGCCTGAGCGTCAAGTTCAGTGGCCGAACCATACCCATCACAAATCTCTCACCGGACTCGACCATCAAGGACTTCAAGGCCCTTCTTCAACCTCTCACCAATGTCCTCCCTCGCGGCCAAAAGCTCATATCCAAAGGGAAGCTTTTGGTGGATGCAATGACGCTGAGGGAGTCTGAGATCACTAATGGGGCCAAGATCATGCTCATGGCTTCTCAGGGTTTGCACCAAGGGGATGGACCTATTCTTAAACAAGCTCAGAGTCGCCCCATTTCTAGAGACAACAATGCTGATAGATCCGATAAAGGAAAGGCGAAAAAAGCTGCTGTTGATAAGAATAGGTTGGAACGATGGAAGGCTACTGGAGTTATAGGACTCAGTGAGTGTAACTTGAAGGCTATACCCGAGGAAGTCTGGGGGGATTCTAGAACTTCTGCAAGAGTTCTTGACCTCAGCAACAATGTTATTCAAGAAGTACCCGCCTTGATTGGCTGTATGAATTCCTTGCAGAAACTTTTACTCGATTCAAACGATATATCGGATGACTCTGTTAGCTGGGAAGCACTAAAATCTTTAAAGTCTTTAACTGTTCTTTCTCTGAACCAAAACCGTTTAACTAGATTGTCATCGGATCTGGGCTGGTTGCCATCACTAAGGCAACTTCATGTTGCGAACAACATGTTGTCTAGCCTCCCAAGTGAATTGCGATATCTGCCTAAGCTTGAAGTTCTGAAAGTTAACAATAACAGGATCACCACCATTCCTCCATGGATAGGGGACCTTATTTCTCTTATTGAGGTTGATCTTTCATCAAATCTTCTGTCAGATTTGCCCGAGACATTTAGCAATTTGCATAATCTAAAGTCCCTGTCTCTCAGTAATAATGGACTGAAATCTCTCCCTTCTTCGCTATTCAAAATGTGCCTAATGCTCACAAGACTTGACTTGCACAACACAGAAATAACCATGGATATCCTTCGTCAGATTGAGGGATGGGAAAACTTTGATGAACGCCGTCGCTTGAAGCAACAGAAGAAACTGGATTTTCGTGTTGTGGGCTCTGCTGCATTTGATGAAGGTGCTGATAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

383

Amino Acids

42.24

Weight (kDa)

8.99

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 23 - 92 2.2e-14 Ubiquitin family
LRR_8 PF13855 144 - 198 9.4e-06 Leucine rich repeat
LRR_14 PF23598 144 - 243 3.9e-06 Leucine-rich repeat region
LRR_14 PF23598 201 - 286 5.8e-08 Leucine-rich repeat region
LRR_8 PF13855 202 - 247 1.2e-06 Leucine rich repeat
LRR_4 PF12799 212 - 252 2.3e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 235 - 293 2.5e-07 Leucine rich repeat
LRR_4 PF12799 235 - 276 5.9e-08 Leucine Rich repeats (2 copies)
LRR_8 PF13855 258 - 316 3.9e-10 Leucine rich repeat
LRR_4 PF12799 258 - 297 1.7e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
AccII CGCG 1 cut(s) 174
AciI CCGC 3 cut(s) 33, 174, 537
AclWI GGATC 3 cut(s) 348, 696, 815
AcoI YGGCCR 2 cut(s) 82, 175
AcsI RAATTY 1 cut(s) 556
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 1 cut(s) 830
AflIII ACRYGT 1 cut(s) 735
AgeI ACCGGT 1 cut(s) 8
AgsI TTSAA 8 cut(s) 136, 149, 451, 527, 585, 782, 973, 1081
AluBI AGCT 7 cut(s) 184, 202, 314, 380, 612, 778, 1149
AluI AGCT 7 cut(s) 184, 202, 314, 380, 612, 778, 1149
Alw26I GTCTC 3 cut(s) 330, 887, 933
AlwI GGATC 3 cut(s) 348, 696, 815
Ama87I CYCGRG 2 cut(s) 461, 890
AoxI GGCC 4 cut(s) 82, 138, 175, 249
ApeKI GCWGC 2 cut(s) 380, 1121
ApoI RAATTY 1 cut(s) 556
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AsiGI ACCGGT 1 cut(s) 8
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 4 cut(s) 139, 249, 296, 835
AsuHPI GGTGA 4 cut(s) 7, 102, 148, 802
AvaI CYCGRG 2 cut(s) 461, 890
AvaII GGWCC 2 cut(s) 296, 835
BanII GRGCYC 1 cut(s) 1118
BbvCI CCTCAGC 2 cut(s) 224, 509
BbvI GCAGC 2 cut(s) 367, 1108
BccI CCATC 6 cut(s) 104, 131, 287, 402, 712, 1044
BceAI ACGGC 1 cut(s) 1056
BcoDI GTCTC 3 cut(s) 330, 887, 933
BfaI CTAG 4 cut(s) 333, 483, 675, 744
BfoI RGCGCY 1 cut(s) 24
BisI GCNGC 3 cut(s) 175, 381, 1122
BlsI GCNGC 3 cut(s) 176, 382, 1123
Bme18I GGWCC 2 cut(s) 296, 835
BmeT110I CYCGRG 2 cut(s) 461, 890
BmgT120I GGNCC 4 cut(s) 139, 249, 296, 835
BmiI GGNNCC 2 cut(s) 250, 836
BmsI GCATC 1 cut(s) 202
BoxI GACNNNNGTC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 441
Bpu10I CCTNAGC 4 cut(s) 65, 224, 509, 774
BsaBI GATNNNNATC 1 cut(s) 682
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 4 cut(s) 286, 462, 824, 1023
BsaWI WCCGGW 2 cut(s) 8, 112
BsaXI ACNNNNNCTCC 2 cut(s) 805, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 830
Bse118I RCCGGY 2 cut(s) 8, 17
Bse1I ACTGG 2 cut(s) 424, 1101
Bse3DI GCAATG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 682
BseDI CCNNGG 4 cut(s) 286, 462, 824, 1023
BseGI GGATG 4 cut(s) 217, 298, 604, 1055
BseJI GATNNNNATC 1 cut(s) 682
BseLI CCNNNNNNNGG 1 cut(s) 830
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 8 cut(s) 56, 215, 225, 287, 329, 448, 523, 946
BseNI ACTGG 2 cut(s) 424, 1101
BseXI GCAGC 2 cut(s) 367, 1108
BseYI CCCAGC 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 4 cut(s) 84, 140, 177, 251
BshTI ACCGGT 1 cut(s) 8
BsiHKCI CYCGRG 2 cut(s) 461, 890
BsiSI CCGG 3 cut(s) 9, 18, 113
BslFI GGGAC 2 cut(s) 848, 907
BslI CCNNNNNNNGG 1 cut(s) 830
BsmAI GTCTC 3 cut(s) 330, 887, 933
BsmFI GGGAC 2 cut(s) 848, 907
BsnI GGCC 4 cut(s) 84, 140, 177, 251
BsoBI CYCGRG 2 cut(s) 461, 890
Bsp1286I GDGCHC 1 cut(s) 1118
Bsp143I GATC 6 cut(s) 237, 255, 353, 688, 807, 859
Bsp19I CCATGG 2 cut(s) 824, 1023
BspACI CCGC 3 cut(s) 33, 174, 537
BspANI GGCC 4 cut(s) 84, 140, 177, 251
BspCNI CTCAG 8 cut(s) 57, 216, 226, 286, 328, 447, 522, 945
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 2 cut(s) 250, 836
BspPI GGATC 3 cut(s) 348, 696, 815
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 2 cut(s) 8, 17
BsrI ACTGG 2 cut(s) 424, 1101
BssAI RCCGGY 2 cut(s) 8, 17
BssECI CCNNGG 4 cut(s) 286, 462, 824, 1023
BssMI GATC 6 cut(s) 237, 255, 353, 688, 807, 859
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 3 cut(s) 286, 824, 1023
Bst4CI ACNGT 2 cut(s) 646, 668
BstACI GRCGYC 1 cut(s) 27
BstDSI CCRYGG 2 cut(s) 824, 1023
BstF5I GGATG 4 cut(s) 217, 298, 604, 1055
BstFNI CGCG 1 cut(s) 174
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 6 cut(s) 240, 258, 356, 691, 810, 862
BstMAI GTCTC 3 cut(s) 330, 887, 933
BstMBI GATC 6 cut(s) 237, 255, 353, 688, 807, 859
BstMWI GCNNNNNNNGC 2 cut(s) 377, 769
BstNSI RCATGY 1 cut(s) 739
BstPAI GACNNNNGTC 1 cut(s) 11
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 2 cut(s) 367, 1108
BstX2I RGATCY 2 cut(s) 353, 688
BstXI CCANNNNNNTGG 1 cut(s) 293
BstYI RGATCY 2 cut(s) 353, 688
BsuRI GGCC 4 cut(s) 84, 140, 177, 251
BtgI CCRYGG 2 cut(s) 824, 1023
BtsCI GGATG 4 cut(s) 217, 298, 604, 1055
BtsIMutI CAGTG 2 cut(s) 85, 442
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 2 cut(s) 8, 17
Cfr13I GGNCC 4 cut(s) 139, 249, 296, 835
CseI GACGC 2 cut(s) 58, 229
Csp6I GTAC 1 cut(s) 533
CspAI ACCGGT 1 cut(s) 8
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 6 cut(s) 259, 265, 722, 736, 825, 1024
CviQI GTAC 1 cut(s) 533
DpnI GATC 6 cut(s) 239, 257, 355, 690, 809, 861
DpnII GATC 6 cut(s) 237, 255, 353, 688, 807, 859
DraI TTTAAA 1 cut(s) 633
EaeI YGGCCR 2 cut(s) 82, 175
Eco130I CCWWGG 3 cut(s) 286, 824, 1023
Eco24I GRGCYC 1 cut(s) 1118
Eco32I GATATC 2 cut(s) 767, 1030
Eco47I GGWCC 2 cut(s) 296, 835
Eco88I CYCGRG 2 cut(s) 461, 890
EcoO109I RGGNCCY 2 cut(s) 139, 835
EcoRI GAATTC 1 cut(s) 556
EcoRV GATATC 2 cut(s) 767, 1030
EcoT14I CCWWGG 3 cut(s) 286, 824, 1023
EcoT38I GRGCYC 1 cut(s) 1118
ErhI CCWWGG 3 cut(s) 286, 824, 1023
FaeI CATG 6 cut(s) 262, 268, 725, 739, 828, 1027
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 2 cut(s) 848, 907
FatI CATG 6 cut(s) 258, 264, 721, 735, 824, 1023
FauI CCCGC 1 cut(s) 544
Fnu4HI GCNGC 3 cut(s) 175, 381, 1122
FokI GGATG 4 cut(s) 224, 305, 611, 1062
FriOI GRGCYC 1 cut(s) 1118
Fsp4HI GCNGC 3 cut(s) 175, 381, 1122
FspBI CTAG 4 cut(s) 333, 483, 675, 744
GlaI GCGC 1 cut(s) 22
GluI GCNGC 3 cut(s) 175, 381, 1122
GsaI CCCAGC 1 cut(s) 616
GsuI CTGGAG 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 4 cut(s) 84, 140, 177, 251
HapII CCGG 3 cut(s) 9, 18, 113
HgaI GACGC 2 cut(s) 58, 229
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 6 cut(s) 262, 268, 725, 739, 828, 1027
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 2 cut(s) 200, 776
HinfI GANTC 7 cut(s) 116, 230, 319, 432, 479, 581, 602
HpaI GTTAAC 1 cut(s) 796
HpaII CCGG 3 cut(s) 9, 18, 113
HphI GGTGA 4 cut(s) 7, 102, 148, 802
Hpy166II GTNNAC 1 cut(s) 796
Hpy188I TCNGA 9 cut(s) 235, 318, 358, 598, 657, 688, 789, 883, 1041
Hpy188III TCNNGA 4 cut(s) 333, 483, 503, 527
Hpy8I GTNNAC 1 cut(s) 796
Hpy99I CGWCG 1 cut(s) 1077
HpyAV CCTTC 6 cut(s) 152, 406, 445, 969, 1043, 1127
HpyCH4III ACNGT 2 cut(s) 646, 668
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 7 cut(s) 215, 283, 494, 565, 910, 1006, 1124
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 769
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 6 cut(s) 262, 268, 725, 739, 828, 1027
HspAI GCGC 1 cut(s) 21
KspAI GTTAAC 1 cut(s) 796
Kzo9I GATC 6 cut(s) 237, 255, 353, 688, 807, 859
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 2 cut(s) 367, 1108
LweI GCATC 1 cut(s) 202
MaeI CTAG 4 cut(s) 333, 483, 675, 744
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 13, 443
MalI GATC 6 cut(s) 239, 257, 355, 690, 809, 861
MboI GATC 6 cut(s) 237, 255, 353, 688, 807, 859
MboII GAAGA 4 cut(s) 137, 866, 954, 1102
MflI RGATCY 2 cut(s) 353, 688
MhlI GDGCHC 1 cut(s) 1118
MluCI AATT 3 cut(s) 556, 758, 904
MlyI GAGTC 5 cut(s) 110, 239, 328, 426, 596
MmeI TCCRAC 1 cut(s) 381
MseI TTAA 5 cut(s) 306, 632, 641, 671, 795
MslI CAYNNNNRTG 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 3 cut(s) 9, 18, 113
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 377, 769
NcoI CCATGG 2 cut(s) 824, 1023
NdeII GATC 6 cut(s) 237, 255, 353, 688, 807, 859
NlaIII CATG 6 cut(s) 262, 268, 725, 739, 828, 1027
NlaIV GGNNCC 2 cut(s) 250, 836
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 1 cut(s) 739
PciI ACATGT 1 cut(s) 735
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PfeI GAWTC 2 cut(s) 479, 581
PinAI ACCGGT 1 cut(s) 8
PkrI GCNGC 3 cut(s) 176, 382, 1123
PleI GAGTC 5 cut(s) 110, 238, 327, 426, 596
PpsI GAGTC 5 cut(s) 110, 238, 327, 426, 596
PpuMI RGGWCCY 1 cut(s) 835
PscI ACATGT 1 cut(s) 735
PshAI GACNNNNGTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 835
PspFI CCCAGC 1 cut(s) 612
PspN4I GGNNCC 2 cut(s) 250, 836
PspPI GGNCC 4 cut(s) 139, 249, 296, 835
PspPPI RGGWCCY 1 cut(s) 835
PsuI RGATCY 2 cut(s) 353, 688
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 5 cut(s) 306, 632, 641, 671, 795
SatI GCNGC 3 cut(s) 175, 381, 1122
Sau3AI GATC 6 cut(s) 237, 255, 353, 688, 807, 859
Sau96I GGNCC 4 cut(s) 139, 249, 296, 835
SchI GAGTC 5 cut(s) 110, 239, 328, 426, 596
SduI GDGCHC 1 cut(s) 1118
SfaNI GCATC 1 cut(s) 202
SgrAI CRCCGGYG 1 cut(s) 17
SinI GGWCC 2 cut(s) 296, 835
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 3 cut(s) 556, 758, 904
SsiI CCGC 3 cut(s) 33, 174, 537
SspMI CTAG 4 cut(s) 333, 483, 675, 744
StyI CCWWGG 3 cut(s) 286, 824, 1023
TaaI ACNGT 2 cut(s) 646, 668
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 119, 579
TasI AATT 3 cut(s) 556, 758, 904
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 2 cut(s) 479, 581
Tru1I TTAA 5 cut(s) 306, 632, 641, 671, 795
Tru9I TTAA 5 cut(s) 306, 632, 641, 671, 795
TscAI CASTG 2 cut(s) 85, 442
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 2 cut(s) 380, 1121
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 5 cut(s) 569, 710, 855, 1080, 1146
TspRI CASTG 2 cut(s) 85, 442
VpaK11BI GGWCC 2 cut(s) 296, 835
XapI RAATTY 1 cut(s) 556
XbaI TCTAGA 2 cut(s) 332, 482
XceI RCATGY 1 cut(s) 739
XspI CTAG 4 cut(s) 333, 483, 675, 744
ZraI GACGTC 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.