Rh5CG506500

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009438
Physical Location & Seq
Forward (+)
70497573 .. 70501832
4260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG506500.1

Sequence Viewer

Length: 636 bp
ATGGCTATTCCCGAGGAAGTCTGGGGGGATTCTAGAACTTCTGCAAGAGTTCTTGACCTCAGCAACAATGTTATTCAAGAAGTACCTGCCTTGATTGGCTGTATGAATTCCTTGCAGAAACTTTTACTCGATTCAAATTATATATCGGACGACTCTGTTAGCTGGGAAGCACTAAAATCTTTAAAGTCTTTAACTGTTCTTTCTCTGAACCAAAACCGTTTAACTAGATTGTCATCGGATCTGGGCTGGTTGCCATCATTAAGGCAACTTCATGTTGCGAACAACATGTTGTCTGGCCTCCCAAGTGAATTGCGATATCTGCCTAAGCTTGAAGTTCTGAAAGTCAACAGTAACAGGATCACCACCATTCCTCCATGGATAGGGGACCTTATTTCTCTTATTGAGTCCCTGTCTGTCAGTAATAATGGGCTGAAATCTCTCCCTTCTTCGCTATTCAAAATGTGCCTAATGCTCACAAGACTTGACTTGCACAACACAGAAATAACCATGGATATCCTTCGTCAGATTGAGGGATGGGAAAACTTTGATGAACGCCGTCGCTTGAAGCAACAGAAGAAACTGGATTTTCGTGTTGTGGGCTCTGCTGCATTTGATGAAGGTGCTGATAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

211

Amino Acids

23.78

Weight (kDa)

6.16

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 17 - 93 1e-05 Leucine-rich repeat region
LRR_8 PF13855 52 - 97 4.9e-07 Leucine rich repeat
LRR_14 PF23598 52 - 164 1.2e-07 Leucine-rich repeat region
LRR_4 PF12799 62 - 100 2.6e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 85 - 126 5.7e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 94
AclWI GGATC 2 cut(s) 246, 365
AcsI RAATTY 1 cut(s) 106
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 380
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 5 cut(s) 77, 135, 332, 457, 565
AluBI AGCT 3 cut(s) 162, 328, 633
AluI AGCT 3 cut(s) 162, 328, 633
AlwI GGATC 2 cut(s) 246, 365
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 385
AsuHPI GGTGA 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 385
BanII GRGCYC 1 cut(s) 602
BbvCI CCTCAGC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 592
BccI CCATC 2 cut(s) 262, 528
BceAI ACGGC 1 cut(s) 540
BfaI CTAG 2 cut(s) 33, 225
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme18I GGWCC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 386
Bpu10I CCTNAGC 2 cut(s) 59, 324
BsaBI GATNNNNATC 1 cut(s) 232
BsaJI CCNNGG 3 cut(s) 12, 374, 507
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 585
Bse8I GATNNNNATC 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 12, 374, 507
BseGI GGATG 1 cut(s) 539
BseJI GATNNNNATC 1 cut(s) 232
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 585
BseXI GCAGC 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 162
BshFI GGCC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 11
BslFI GGGAC 2 cut(s) 391, 398
BslI CCNNNNNNNGG 1 cut(s) 380
BsmFI GGGAC 2 cut(s) 391, 398
BsnI GGCC 1 cut(s) 297
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 602
Bsp143I GATC 2 cut(s) 238, 357
Bsp19I CCATGG 2 cut(s) 374, 507
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 386
BspMI ACCTGC 1 cut(s) 94
BspPI GGATC 2 cut(s) 246, 365
BsrI ACTGG 1 cut(s) 585
BssECI CCNNGG 3 cut(s) 12, 374, 507
BssMI GATC 2 cut(s) 238, 357
BssT1I CCWWGG 2 cut(s) 374, 507
Bst4CI ACNGT 3 cut(s) 196, 218, 350
BstDEI CTNAG 2 cut(s) 59, 324
BstDSI CCRYGG 2 cut(s) 374, 507
BstF5I GGATG 1 cut(s) 539
BstKTI GATC 2 cut(s) 241, 360
BstMBI GATC 2 cut(s) 238, 357
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 592
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 1 cut(s) 297
BtgI CCRYGG 2 cut(s) 374, 507
BtsCI GGATG 1 cut(s) 539
BveI ACCTGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 83
CviAII CATG 4 cut(s) 272, 286, 375, 508
CviJI RGCY 9 cut(s) 5, 99, 162, 246, 297, 328, 430, 600, 633
CviKI_1 RGCY 9 cut(s) 5, 99, 162, 246, 297, 328, 430, 600, 633
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 2 cut(s) 59, 324
DpnI GATC 2 cut(s) 240, 359
DpnII GATC 2 cut(s) 238, 357
DraI TTTAAA 1 cut(s) 183
Eco130I CCWWGG 2 cut(s) 374, 507
Eco24I GRGCYC 1 cut(s) 602
Eco32I GATATC 2 cut(s) 317, 514
Eco47I GGWCC 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 385
EcoRI GAATTC 1 cut(s) 106
EcoRV GATATC 2 cut(s) 317, 514
EcoT14I CCWWGG 2 cut(s) 374, 507
EcoT38I GRGCYC 1 cut(s) 602
ErhI CCWWGG 2 cut(s) 374, 507
FaeI CATG 4 cut(s) 275, 289, 378, 511
FaiI YATR 7 cut(s) 104, 141, 143, 273, 287, 376, 509
FaqI GGGAC 2 cut(s) 391, 398
FatI CATG 4 cut(s) 271, 285, 374, 507
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 1 cut(s) 546
FriOI GRGCYC 1 cut(s) 602
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 2 cut(s) 33, 225
GluI GCNGC 1 cut(s) 606
GsaI CCCAGC 1 cut(s) 166
HaeIII GGCC 1 cut(s) 297
Hin1II CATG 4 cut(s) 275, 289, 378, 511
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 4 cut(s) 29, 131, 152, 404
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 5 cut(s) 148, 207, 238, 339, 525
Hpy188III TCNNGA 4 cut(s) 11, 33, 53, 77
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 1 cut(s) 561
HpyAV CCTTC 3 cut(s) 453, 527, 611
HpyCH4III ACNGT 3 cut(s) 196, 218, 350
HpyCH4V TGCA 4 cut(s) 44, 115, 490, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
HpyF3I CTNAG 2 cut(s) 59, 324
Hsp92II CATG 4 cut(s) 275, 289, 378, 511
Kzo9I GATC 2 cut(s) 238, 357
LpnPI CCDG 9 cut(s) 7, 99, 148, 227, 232, 279, 340, 422, 566
Lsp1109I GCAGC 1 cut(s) 592
MaeI CTAG 2 cut(s) 33, 225
MaeIII GTNAC 1 cut(s) 350
MalI GATC 2 cut(s) 240, 359
MboI GATC 2 cut(s) 238, 357
MboII GAAGA 2 cut(s) 438, 586
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 602
MluCI AATT 3 cut(s) 106, 136, 308
MlyI GAGTC 2 cut(s) 146, 413
MnlI CCTC 5 cut(s) 7, 68, 308, 381, 523
MseI TTAA 4 cut(s) 182, 191, 221, 260
MwoI GCNNNNNNNGC 1 cut(s) 319
NcoI CCATGG 2 cut(s) 374, 507
NdeII GATC 2 cut(s) 238, 357
NlaIII CATG 4 cut(s) 275, 289, 378, 511
NlaIV GGNNCC 1 cut(s) 386
NspI RCATGY 1 cut(s) 289
PciI ACATGT 1 cut(s) 285
PfeI GAWTC 2 cut(s) 29, 131
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 2 cut(s) 146, 412
PpsI GAGTC 2 cut(s) 146, 412
PpuMI RGGWCCY 1 cut(s) 385
PscI ACATGT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 386
PspPI GGNCC 1 cut(s) 385
PspPPI RGGWCCY 1 cut(s) 385
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
SaqAI TTAA 4 cut(s) 182, 191, 221, 260
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 2 cut(s) 238, 357
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 2 cut(s) 146, 413
SduI GDGCHC 1 cut(s) 602
SetI ASST 7 cut(s) 60, 88, 164, 330, 390, 622, 635
SinI GGWCC 1 cut(s) 385
Sse9I AATT 3 cut(s) 106, 136, 308
SspMI CTAG 2 cut(s) 33, 225
StyI CCWWGG 2 cut(s) 374, 507
TaaI ACNGT 3 cut(s) 196, 218, 350
TaqI TCGA 1 cut(s) 129
TasI AATT 3 cut(s) 106, 136, 308
TfiI GAWTC 2 cut(s) 29, 131
Tru1I TTAA 4 cut(s) 182, 191, 221, 260
Tru9I TTAA 4 cut(s) 182, 191, 221, 260
TseI GCWGC 1 cut(s) 605
TspDTI ATGAA 4 cut(s) 119, 260, 564, 630
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 1 cut(s) 106
XbaI TCTAGA 1 cut(s) 32
XceI RCATGY 1 cut(s) 289
XspI CTAG 2 cut(s) 33, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.