FvH4_4g00520

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001113
Physical Location & Seq
Forward (+)
466458 .. 474236
7779 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00520.t2

Sequence Viewer

Length: 2286 bp
ATGCTTGATGTGTACATTTATGATTATCTAATGAAGAAAGAATTGCATGCCACTGCCAAGTCATTCATGAATGAAGGGAGGGTTGCCCCGGATCCAGTAGCCATTGATGCGCCTGGAGGGTTTCTTTTTGAATGGTGGTCTGTCTTCTGGGACATTTTTATTGCAAGGACAAATGATAAACATTCCGAGTCTGCAGCAGTTTATATAGAGGCACAACAAATTAAAGCCAAAGAGCAACAAATGCAGCAGTTGCAACTAATGCGCCAAGCTCAGTTGCAACGAACTCCTAGTAATCCTCCCCTTGGTGGTGCACTTAATTCACTAAGTTCTGAAGGAATGCTTGGGCAATCTGCTTCTAGTGCATTGGCCGCAAAAATGTATGAGGAACGAATGAAACACCCCAATCAGATAGATTCAGAGACATCCCAACCACTCCTTGATGCTAGAGCGGCCCTGCTGAAATCAACCACAAACCATCCTGGTCAGTTGGTTCAAGGGAACCCTGGTAATGTTAATCCGGCATTACAACAAATCCAAGCTCGAAATCAACAGACTGCTGACATAAAGAGTGAAATTAATATGGGTGCCTCTCAGAGATCTTTGCCTGTGGATTCTTCAATTTATGCACAAGGAATGATGCAGCCGAAACCAGTTATAGGAAATTCAGGTTTAAACCCAGGAGTCAGTGGTCTTCCATTAAAGGGATGGCCCTTAACAGGGATCGACCAAATGCAGCCAGGTTTAGGTGCACAAGTTCAAAAACCTTTCCTGCAAGGTGCAACTCAGTTTCAGATCCTCTCACAGCAACAACAACAACTTTTAGCACAGGTTCAGACACAAGGCAATGTTGGTAGCACACCTGGCTATGGAGATATGAGGGGACTTCCCAGGGGAAATTTAAACTCTAAAGATGGCCAACCTATAATAAACAATGGATCTATAGGTTCTACTATGCAAACAACTACACAGATAAATATGCCACAGATGCAACACTCTTCTTCTCAACAACAGGATCCTTTGCAGTCTCAGCAAGTACAGCAGAATAACCGCAAAAGGAAAGGACCTTCATCCTCTGGAGCTGCTAACAGTACCGGTACAGGAAATACAGTTGGTCCTTCATCTAATTCCCAACCTTCAACTCCATCCACTCATACCCCCGGAGATGGAATTGCTCTGGCTGGAAATTTGCCGAATGCTAGCAATGTTCCAAAAAGTTTGATGATGTATGGCACGGATGAAACAGGTGGCCTTGTATCATCAACAAATCAGCTGGAAGATATTGACCAATTTGGAGATGTTGGTTCCCTAGAAGACAATGTGGAATCCTTTTTGTCACATGATGATGGAGAAGGAAGGGATTTGTTTGGTACATTGAACGGGAACCCTGCACATTCTGCAGCAGCTTCAAAGGGTTTTTCCTTCACTGAAGTTGGTTCTATATGTAAAAGTAGTAGCAAAGTTGTCTGCTGTCACTTCTCTTCAGATGGAAAATTGTTGGCCAGTGCTGGACATGACAAGAAGGTTGTTGTTTGGAATATGGATACACTACAGACTGAAAGCACACTGGAGGATCACAGTTTGATAATTACTGATGTTCGATTTAGACCAAATTCAACTCAGTTGGCAACTTCTTCATTTGACACAACTGTGCGACTTTGGGATGCTGCTGAACCAAACTATTGTTTACAGACATATAAAGGACATACCTCGCATGTAATGTCACTTGATTTCCACCCTAAGAAGACTGACCTTTTCTGCTCATGTGACACGAACAATGAGATTCGCTTTTGGAATATTAATCAGTATTCGTGTACTCGTGTTATTAAGGGAGGTTCATCACAGGTGAGGTTCCAGCCAAGAATTGGACAATTTATGGCTGCAGCATCTGGGAATGTTGTGTCAATATTTGATGCTGAGAGTGATAGGCAGACACATTCACTGCAGGGCCACTCCACAGAGGTGCACTCTCTTTGTTGGGATACAAATGGAGATTATTTGAGTTCAGTCAGTCAAGAGTCTGTGAGAGTGTGGTCATTATCGACTGGGAAGTGCATTCACGAGCTCAGTTCTAGTGGAAACATGTTCCATTCTTGTGTTTTCCATCCAAGCTACTCCACTCTCTTGGTCATTGGAGGCTACCAGTCAATGGAGCTCTGGAACATGGCTGATAACAAATGTATGACTGTTGCTGCTCATGACTGTGTAATATCAGCTTTGGCACAGTCACCAGTTACAGGGATGGTAGCTTCAGCCAGTCATGATAAATCCGTTAAAATCTGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

762

Amino Acids

82.38

Weight (kDa)

5.99

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 10 - 36 8.8e-07 LisH
WD40_WDHD1_1st PF24817 479 - 569 2.3e-13 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 485 - 689 1.4e-14 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 485 - 651 1.7e-12 G protein beta WD-40 repeat protein
WDR55 PF24796 486 - 611 1.2e-07 WDR55
Beta-prop_EML_2 PF23414 491 - 612 1.6e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 491 - 616 1.2e-10 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 491 - 591 4.5e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 492 - 625 1.9e-15 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 493 - 625 7.1e-30 WDR5 beta-propeller domain
WD40 PF00400 494 - 522 7.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 494 - 609 4.6e-23 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 494 - 616 5.4e-06 MABP1/WDR62 first WD40 domain
Beta-prop_THOC3 PF25174 494 - 568 1e-15 THOC3 beta-propeller domain
WD40_Prp19 PF24814 495 - 731 1.5e-37 Prp19 WD40 domain
Beta-prop_EML PF23409 497 - 677 5.4e-09 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 500 - 566 4.3e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 500 - 596 1.8e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 507 - 609 1.6e-06 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 526 - 681 6.9e-17 CDC20/Fizzy WD40 domain
WD40 PF00400 529 - 564 5.1e-07 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 573 - 719 2.6e-14 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 573 - 771 2.1e-28 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 596 - 699 5.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 598 - 771 5.2e-20 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 622 - 770 1e-22 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 633 - 768 3.1e-11 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 633 - 770 2.9e-11 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 642 - 736 1e-05 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 663 - 771 1.8e-09 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 584
AccB7I CCANNNNNTGG 2 cut(s) 1862, 2146
AccBSI CCGCTC 1 cut(s) 449
AciI CCGC 3 cut(s) 369, 449, 1048
AclWI GGATC 8 cut(s) 86, 99, 728, 787, 943, 1007, 1020, 1578
AcoI YGGCCR 3 cut(s) 366, 913, 1497
AcsI RAATTY 4 cut(s) 661, 895, 1183, 1609
AcuI CTGAAG 4 cut(s) 351, 1446, 1464, 2232
AfaI GTAC 6 cut(s) 14, 1035, 1090, 1096, 1369, 1813
AflIII ACRYGT 1 cut(s) 2079
AgeI ACCGGT 1 cut(s) 1091
AgsI TTSAA 8 cut(s) 131, 494, 618, 758, 1137, 1375, 1407, 1614
AjnI CCWGG 7 cut(s) 112, 478, 502, 676, 736, 859, 887
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AleI CACNNNNGTG 2 cut(s) 1646, 1958
Alw21I GWGCWC 5 cut(s) 313, 751, 1965, 2064, 2154
Alw26I GTCTC 2 cut(s) 413, 1029
Alw44I GTGCAC 3 cut(s) 309, 747, 1961
AlwI GGATC 8 cut(s) 86, 99, 728, 787, 943, 1007, 1020, 1578
AlwNI CAGNNNCTG 1 cut(s) 1886
AoxI GGCC 7 cut(s) 366, 450, 707, 913, 1246, 1497, 1945
ApaLI GTGCAC 3 cut(s) 309, 747, 1961
ApoI RAATTY 4 cut(s) 661, 895, 1183, 1609
AseI ATTAAT 2 cut(s) 576, 1797
AsiGI ACCGGT 1 cut(s) 1091
AspLEI GCGC 2 cut(s) 112, 264
AspS9I GGNCC 5 cut(s) 451, 708, 1061, 1112, 1945
AsuC2I CCSGG 2 cut(s) 89, 1158
AsuHPI GGTGA 2 cut(s) 1855, 2217
AsuNHI GCTAGC 1 cut(s) 1196
AvaII GGWCC 2 cut(s) 1061, 1112
BaeGI GKGCMC 3 cut(s) 313, 751, 1965
BalI TGGCCA 2 cut(s) 915, 1499
BamHI GGATCC 2 cut(s) 91, 1012
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 2 cut(s) 2064, 2154
BauI CACGAG 2 cut(s) 1815, 2057
BbsI GAAGAC 4 cut(s) 136, 683, 1317, 1748
Bbv12I GWGCWC 5 cut(s) 313, 751, 1965, 2064, 2154
BccI CCATC 9 cut(s) 483, 699, 905, 1150, 1157, 1337, 1478, 2109, 2233
BciT130I CCWGG 7 cut(s) 114, 480, 504, 678, 738, 861, 889
BciVI GTATCC 2 cut(s) 1534, 1972
BcnI CCSGG 2 cut(s) 89, 1158
BcoDI GTCTC 2 cut(s) 413, 1029
BfaI CTAG 6 cut(s) 288, 357, 444, 1197, 1307, 2070
BfmI CTRYAG 6 cut(s) 192, 939, 1395, 1547, 1878, 1940
BfuI GTATCC 2 cut(s) 1534, 1972
BglII AGATCT 1 cut(s) 596
Bme1390I CCNGG 9 cut(s) 89, 114, 480, 504, 678, 738, 861, 889, 1158
Bme18I GGWCC 2 cut(s) 1061, 1112
BmgT120I GGNCC 5 cut(s) 451, 708, 1061, 1112, 1945
BmiI GGNNCC 7 cut(s) 93, 500, 586, 1014, 1303, 1382, 1850
BmrFI CCNGG 9 cut(s) 89, 114, 480, 504, 678, 738, 861, 889, 1158
BmrI ACTGGG 1 cut(s) 2052
BmsI GCATC 7 cut(s) 97, 430, 627, 975, 1651, 1892, 1900
BmtI GCTAGC 1 cut(s) 1200
BmuI ACTGGG 1 cut(s) 2052
BpiI GAAGAC 4 cut(s) 136, 683, 1317, 1748
BplI GAGNNNNNCTC 2 cut(s) 1949, 1981
BpmI CTGGAG 3 cut(s) 135, 1095, 1586
BpuMI CCSGG 2 cut(s) 89, 1158
BsaJI CCNNGG 7 cut(s) 87, 301, 502, 676, 887, 888, 1156
BsaWI WCCGGW 1 cut(s) 1091
Bse118I RCCGGY 1 cut(s) 1091
Bse1I ACTGG 8 cut(s) 95, 650, 1500, 1569, 2047, 2140, 2228, 2253
Bse3DI GCAATG 2 cut(s) 850, 1207
BseBI CCWGG 7 cut(s) 114, 480, 504, 678, 738, 861, 889
BseDI CCNNGG 7 cut(s) 87, 301, 502, 676, 887, 888, 1156
BseGI GGATG 9 cut(s) 422, 475, 710, 1067, 1142, 1240, 1666, 2101, 2244
BseMI GCAATG 2 cut(s) 850, 1207
BseMII CTCAG 7 cut(s) 284, 605, 797, 1040, 1631, 1905, 2077
BseNI ACTGG 8 cut(s) 95, 650, 1500, 1569, 2047, 2140, 2228, 2253
BseSI GKGCMC 3 cut(s) 313, 751, 1965
BsgI GTGCAG 1 cut(s) 1371
BshFI GGCC 7 cut(s) 368, 452, 709, 915, 1248, 1499, 1947
BshNI GGYRCC 1 cut(s) 584
BshTI ACCGGT 1 cut(s) 1091
BsiHKAI GWGCWC 5 cut(s) 313, 751, 1965, 2064, 2154
BsiSI CCGG 4 cut(s) 89, 518, 1092, 1158
BslFI GGGAC 2 cut(s) 164, 894
BsmAI GTCTC 2 cut(s) 413, 1029
BsmFI GGGAC 2 cut(s) 164, 894
BsmI GAATGC 3 cut(s) 342, 1198, 2052
BsnI GGCC 7 cut(s) 368, 452, 709, 915, 1248, 1499, 1947
Bsp1286I GDGCHC 5 cut(s) 313, 751, 1965, 2064, 2154
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
BspACI CCGC 3 cut(s) 369, 449, 1048
BspANI GGCC 7 cut(s) 368, 452, 709, 915, 1248, 1499, 1947
BspCNI CTCAG 7 cut(s) 283, 604, 796, 1039, 1630, 1906, 2076
BspHI TCATGA 3 cut(s) 66, 2194, 2257
BspLI GGNNCC 7 cut(s) 93, 500, 586, 1014, 1303, 1382, 1850
BspMAI CTGCAG 4 cut(s) 196, 1399, 1882, 1944
BspOI GCTAGC 1 cut(s) 1200
BspPI GGATC 8 cut(s) 86, 99, 728, 787, 943, 1007, 1020, 1578
BspT107I GGYRCC 1 cut(s) 584
BsrBI CCGCTC 1 cut(s) 449
BsrDI GCAATG 2 cut(s) 850, 1207
BsrFI RCCGGY 1 cut(s) 1091
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 8 cut(s) 95, 650, 1500, 1569, 2047, 2140, 2228, 2253
BssAI RCCGGY 1 cut(s) 1091
BssECI CCNNGG 7 cut(s) 87, 301, 502, 676, 887, 888, 1156
BssMI GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
BssSI CACGAG 2 cut(s) 1815, 2057
BssT1I CCWWGG 1 cut(s) 301
Bst2BI CACGAG 2 cut(s) 1815, 2057
Bst2UI CCWGG 7 cut(s) 114, 480, 504, 678, 738, 861, 889
Bst4CI ACNGT 7 cut(s) 1088, 1108, 1577, 1648, 2185, 2201, 2223
Bst6I CTCTTC 2 cut(s) 1000, 1483
BstAPI GCANNNNNTGC 4 cut(s) 241, 250, 259, 1394
BstAUI TGTACA 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 48, 1198
BstDEI CTNAG 9 cut(s) 270, 323, 591, 783, 1026, 1617, 1737, 1914, 2063
BstF5I GGATG 9 cut(s) 422, 475, 710, 1067, 1142, 1240, 1666, 2101, 2244
BstHHI GCGC 2 cut(s) 112, 264
BstKTI GATC 7 cut(s) 94, 599, 723, 795, 938, 1015, 1573
BstMAI GTCTC 2 cut(s) 413, 1029
BstMBI GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
BstNI CCWGG 7 cut(s) 114, 480, 504, 678, 738, 861, 889
BstNSI RCATGY 3 cut(s) 50, 1715, 2083
BstSCI CCNGG 9 cut(s) 87, 112, 478, 502, 676, 736, 859, 887, 1156
BstSFI CTRYAG 6 cut(s) 192, 939, 1395, 1547, 1878, 1940
BstSLI GKGCMC 3 cut(s) 313, 751, 1965
BstV2I GAAGAC 4 cut(s) 136, 683, 1317, 1748
BstX2I RGATCY 5 cut(s) 91, 596, 792, 935, 1012
BstXI CCANNNNNNTGG 1 cut(s) 2122
BstYI RGATCY 5 cut(s) 91, 596, 792, 935, 1012
BsuI GTATCC 2 cut(s) 1534, 1972
BsuRI GGCC 7 cut(s) 368, 452, 709, 915, 1248, 1499, 1947
BtsCI GGATG 9 cut(s) 422, 475, 710, 1067, 1142, 1240, 1666, 2101, 2244
BtsI GCAGTG 2 cut(s) 51, 1937
BtsIMutI CAGTG 6 cut(s) 51, 691, 1422, 1507, 1562, 1937
Cac8I GCNNGC 2 cut(s) 48, 1198
CaiI CAGNNNCTG 1 cut(s) 1886
CciI TCATGA 3 cut(s) 66, 2194, 2257
CfoI GCGC 2 cut(s) 112, 264
Cfr10I RCCGGY 1 cut(s) 1091
Cfr13I GGNCC 5 cut(s) 451, 708, 1061, 1112, 1945
Csp6I GTAC 6 cut(s) 13, 1034, 1089, 1095, 1368, 1812
CspAI ACCGGT 1 cut(s) 1091
CviQI GTAC 6 cut(s) 13, 1034, 1089, 1095, 1368, 1812
DdeI CTNAG 9 cut(s) 270, 323, 591, 783, 1026, 1617, 1737, 1914, 2063
DpnI GATC 7 cut(s) 93, 598, 722, 794, 937, 1014, 1572
DpnII GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
DraI TTTAAA 2 cut(s) 672, 900
EaeI YGGCCR 3 cut(s) 366, 913, 1497
Eam1104I CTCTTC 2 cut(s) 1000, 1483
EarI CTCTTC 2 cut(s) 1000, 1483
Ecl136II GAGCTC 2 cut(s) 2062, 2152
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 2 cut(s) 2064, 2154
Eco47I GGWCC 2 cut(s) 1061, 1112
Eco53kI GAGCTC 2 cut(s) 2062, 2152
Eco57I CTGAAG 4 cut(s) 351, 1446, 1464, 2232
EcoICRI GAGCTC 2 cut(s) 2062, 2152
EcoO109I RGGNCCY 1 cut(s) 1061
EcoRII CCWGG 7 cut(s) 112, 478, 502, 676, 736, 859, 887
EcoT14I CCWWGG 1 cut(s) 301
EcoT38I GRGCYC 2 cut(s) 2064, 2154
ErhI CCWWGG 1 cut(s) 301
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FaqI GGGAC 2 cut(s) 164, 894
FokI GGATG 9 cut(s) 409, 462, 717, 1054, 1129, 1247, 1673, 2088, 2251
FriOI GRGCYC 2 cut(s) 2064, 2154
FspBI CTAG 6 cut(s) 288, 357, 444, 1197, 1307, 2070
GlaI GCGC 2 cut(s) 111, 263
GsuI CTGGAG 3 cut(s) 135, 1095, 1586
HaeIII GGCC 7 cut(s) 368, 452, 709, 915, 1248, 1499, 1947
HapII CCGG 4 cut(s) 89, 518, 1092, 1158
HhaI GCGC 2 cut(s) 112, 264
Hin6I GCGC 2 cut(s) 110, 262
HinP1I GCGC 2 cut(s) 110, 262
HinfI GANTC 7 cut(s) 188, 413, 611, 681, 1322, 1780, 2015
HpaII CCGG 4 cut(s) 89, 518, 1092, 1158
HphI GGTGA 2 cut(s) 1855, 2217
Hpy166II GTNNAC 6 cut(s) 13, 311, 749, 1685, 1812, 1963
Hpy188I TCNGA 8 cut(s) 187, 331, 408, 418, 594, 792, 834, 1483
Hpy188III TCNNGA 8 cut(s) 67, 1074, 2012, 2057, 2155, 2195, 2258, 2278
Hpy8I GTNNAC 6 cut(s) 13, 311, 749, 1685, 1812, 1963
HpyAV CCTTC 9 cut(s) 68, 326, 1074, 1125, 1143, 1343, 1347, 1429, 1513
HpyCH4III ACNGT 7 cut(s) 1088, 1108, 1577, 1648, 2185, 2201, 2223
HpyF3I CTNAG 9 cut(s) 270, 323, 591, 783, 1026, 1617, 1737, 1914, 2063
HspAI GCGC 2 cut(s) 110, 262
Kzo9I GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
LmnI GCTCC 2 cut(s) 1076, 2149
LweI GCATC 7 cut(s) 97, 430, 627, 975, 1651, 1892, 1900
MaeI CTAG 6 cut(s) 288, 357, 444, 1197, 1307, 2070
MaeIII GTNAC 6 cut(s) 1332, 1469, 1719, 1763, 2223, 2230
MalI GATC 7 cut(s) 93, 598, 722, 794, 937, 1014, 1572
MbiI CCGCTC 1 cut(s) 449
MboI GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
MflI RGATCY 5 cut(s) 91, 596, 792, 935, 1012
MhlI GDGCHC 5 cut(s) 313, 751, 1965, 2064, 2154
MlsI TGGCCA 2 cut(s) 915, 1499
MluNI TGGCCA 2 cut(s) 915, 1499
MlyI GAGTC 3 cut(s) 197, 690, 2024
Mox20I TGGCCA 2 cut(s) 915, 1499
MscI TGGCCA 2 cut(s) 915, 1499
MslI CAYNNNNRTG 5 cut(s) 1341, 1646, 1958, 2091, 2199
Msp20I TGGCCA 2 cut(s) 915, 1499
MspA1I CMGCKG 1 cut(s) 1270
MspI CCGG 4 cut(s) 89, 518, 1092, 1158
MspR9I CCNGG 9 cut(s) 89, 114, 480, 504, 678, 738, 861, 889, 1158
MssI GTTTAAAC 1 cut(s) 672
Mva1269I GAATGC 3 cut(s) 342, 1198, 2052
MvaI CCWGG 7 cut(s) 114, 480, 504, 678, 738, 861, 889
NciI CCSGG 2 cut(s) 89, 1158
NdeII GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
NheI GCTAGC 1 cut(s) 1196
NlaIV GGNNCC 7 cut(s) 93, 500, 586, 1014, 1303, 1382, 1850
NmuCI GTSAC 5 cut(s) 1332, 1469, 1719, 1763, 2223
NspI RCATGY 3 cut(s) 50, 1715, 2083
OliI CACNNNNGTG 2 cut(s) 1646, 1958
PaeI GCATGC 1 cut(s) 50
PagI TCATGA 3 cut(s) 66, 2194, 2257
PasI CCCWGGG 1 cut(s) 888
PciI ACATGT 1 cut(s) 2079
PctI GAATGC 3 cut(s) 342, 1198, 2052
PfeI GAWTC 4 cut(s) 413, 611, 1322, 1780
PflMI CCANNNNNTGG 2 cut(s) 1862, 2146
PinAI ACCGGT 1 cut(s) 1091
PleI GAGTC 3 cut(s) 196, 689, 2023
PmeI GTTTAAAC 1 cut(s) 672
PpsI GAGTC 3 cut(s) 196, 689, 2023
PpuMI RGGWCCY 1 cut(s) 1061
PscI ACATGT 1 cut(s) 2079
PshBI ATTAAT 2 cut(s) 576, 1797
Psp124BI GAGCTC 2 cut(s) 2064, 2154
Psp5II RGGWCCY 1 cut(s) 1061
Psp6I CCWGG 7 cut(s) 112, 478, 502, 676, 736, 859, 887
PspGI CCWGG 7 cut(s) 112, 478, 502, 676, 736, 859, 887
PspN4I GGNNCC 7 cut(s) 93, 500, 586, 1014, 1303, 1382, 1850
PspPI GGNCC 5 cut(s) 451, 708, 1061, 1112, 1945
PspPPI RGGWCCY 1 cut(s) 1061
PstI CTGCAG 4 cut(s) 196, 1399, 1882, 1944
PstNI CAGNNNCTG 1 cut(s) 1886
PsuI RGATCY 5 cut(s) 91, 596, 792, 935, 1012
PvuII CAGCTG 1 cut(s) 1270
RsaI GTAC 6 cut(s) 14, 1035, 1090, 1096, 1369, 1813
RsaNI GTAC 6 cut(s) 13, 1034, 1089, 1095, 1368, 1812
RseI CAYNNNNRTG 5 cut(s) 1341, 1646, 1958, 2091, 2199
SacI GAGCTC 2 cut(s) 2064, 2154
Sau3AI GATC 7 cut(s) 91, 596, 720, 792, 935, 1012, 1570
Sau96I GGNCC 5 cut(s) 451, 708, 1061, 1112, 1945
SchI GAGTC 3 cut(s) 197, 690, 2024
ScrFI CCNGG 9 cut(s) 89, 114, 480, 504, 678, 738, 861, 889, 1158
SduI GDGCHC 5 cut(s) 313, 751, 1965, 2064, 2154
SfaNI GCATC 7 cut(s) 97, 430, 627, 975, 1651, 1892, 1900
SfcI CTRYAG 6 cut(s) 192, 939, 1395, 1547, 1878, 1940
SinI GGWCC 2 cut(s) 1061, 1112
SmiMI CAYNNNNRTG 5 cut(s) 1341, 1646, 1958, 2091, 2199
SphI GCATGC 1 cut(s) 50
SsiI CCGC 3 cut(s) 369, 449, 1048
SspI AATATT 2 cut(s) 1795, 1905
SspMI CTAG 6 cut(s) 288, 357, 444, 1197, 1307, 2070
SstI GAGCTC 2 cut(s) 2064, 2154
StyD4I CCNGG 9 cut(s) 87, 112, 478, 502, 676, 736, 859, 887, 1156
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 7 cut(s) 1088, 1108, 1577, 1648, 2185, 2201, 2223
TaqI TCGA 4 cut(s) 541, 723, 1597, 2039
TatI WGTACW 3 cut(s) 12, 1033, 1811
TauI GCSGC 2 cut(s) 371, 452
TfiI GAWTC 4 cut(s) 413, 611, 1322, 1780
TscAI CASTG 6 cut(s) 58, 691, 1429, 1507, 1569, 1944
TseFI GTSAC 5 cut(s) 1332, 1469, 1719, 1763, 2223
Tsp45I GTSAC 5 cut(s) 1332, 1469, 1719, 1763, 2223
TspGWI ACGGA 2 cut(s) 1247, 2257
TspRI CASTG 6 cut(s) 58, 691, 1429, 1507, 1569, 1944
Van91I CCANNNNNTGG 2 cut(s) 1862, 2146
VneI GTGCAC 3 cut(s) 309, 747, 1961
VpaK11BI GGWCC 2 cut(s) 1061, 1112
VspI ATTAAT 2 cut(s) 576, 1797
XapI RAATTY 4 cut(s) 661, 895, 1183, 1609
XceI RCATGY 3 cut(s) 50, 1715, 2083
XcmI CCANNNNNNNNNTGG 2 cut(s) 702, 1859
XspI CTAG 6 cut(s) 288, 357, 444, 1197, 1307, 2070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.