MD05G1176000.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001113
Physical Location & Seq
Reverse (-)
30355057 .. 30361121
6065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1176000.v1.1.491

Sequence Viewer

Length: 2325 bp
ATGGCGCAGAGTAACTGGGAAGCCGATAAGATGCTTGATGTTTATATTCATGACTATTTGTTGAAAAGAAAATTGCTTGCTTCTGCAAATGCGTTTATGACGGAAGGGAAGGTTGCTACAGATCCAGTAGCAATTGATGCACCAGGAGGATTTCTCTTTGAATGGTGGTCTGTCTTTTGGGACATATTCATTGCAAGGACAAATGAGAAGCATTCAGAGGCTGCTGCAGCTTACATTGAGGCACAGCAAATTAAGGCAAGGGAGCAACAGCAGATACAATTGCAGCAGCTGCAGCTGTTGCAGCAGCGCAGTGCTCAGTTACAGCGAAGGGATCCTAGTCATCCTCCTCTTGGTAGTGCTATAAATGCTATAAACTCTGAGGGTGTTATGGGGCAACCATCGGCCAGTGTGCTAGCCATGAAAATGTATGAAGATCGTATGAAACACCCTCACTCAATGGATTCAGAGGCTTCCCCTGCGCTTATTGATGCCAGTAGGATGGCCCTGCTCAAATCAGCTGCAAATCCACAAGGCCAGTTGGTACAAGGCAATTCTGGGAACATGCCAGGTGCTTTGCAGCAAATCCAAGCACGAATTCCTTTAACCACTGACATCAAAAGCGAAGTTAGCTTAGGTGCAACTCAGAAAAGTTTGCCTATGGATCCTTCATCAATTTATGGACAAGCCATTTTACAGTCAAAATCTGGACTAAGCACTGCAGGATTGAATCAAGGTATTACAGGTCTTCCTTTGAAGGGTTGGCCTCTAACTGGAATCAACCAGTTACGACCGAATTTGGGTGTACAAATGCAGAAGCCAAATCTTCAGACCCAAAATCAGTTCCTTTTGGCACCTCAACAACAGCAAGTTTTGGCACAAGGTAATTCCACAAGTTACGGAGATATGGATCCTCGCAGATTTTCTGGACTGCCTAGGGGTGGCTTGAATGCAAAAGATGGTCAATCTACCAGAAATGATGGATCAATGTCCTCTCCAGTTTTATCGAGTTCACCAAAGATAAAAATGGCTCAGATGCAGCACCCTTCCTCTCAACAACATGAACAATTACCACAACAGCAACTACAACAGAAAAACAGAAAGAGGAAACAACACTCTTCCTCTGGACCTGCCAACAGCACCGGCACAGGAAACACTGTTGGCCCTTCACCCAGTTCGCCAGCATCAACTCACACACCTGGTGATGGAATTAACACAGCCAATAGTATGCAGCAAGTCAACAGTATGCCAAAAAGCTTGATGACGTATGGTCCAGAAGGAACAGGACTTCCGTCATCTTCCAATTTACTGGATGATATTGAACGATTTGGAGATGTTGGTTCTTTAGAAGACAATGTGGAATCATTTTTGTCTAATGATGGGGGAGACAGGAGGGATCTGTATGGGACATTAAAGCAGAGTCCTAAACAGCACCAAAAAGATTCTTCTAAAGGTTTTACATTTGGTGAAGTTGGCTGCATACGGACCAGAACCAGCAAAGTTACTTGCTGTCACTTCTCTTCAGATGGGAAGTTGCTGGCTAGTGCTGGACATGACAAAAAGGTTGTCCTCTGGAACATGGATACTCTGCAGACAGAGAGCACTCCCGAAGAACACAAGTTAGTTATCACAGATGTTCGGTTTAGACCAAATTCATCTCAGCTGGCAACAGCTTCAGTTGACAAATCTATACGATTGTGGGATGCAGCCAATCCAAACTATTGTTTACGTGCATATACGGGGCACAACTCACCTATCATGTCCCTTGACTTCCACCCTAAGAAGACTGATTTATTTTGTTTCTGTGATCATGACAATGAAATTCGGTACTGGAATATTAACTCATTCTCTTGTACTCGTAGTTCAAAGGGAGGAACTGCACAAGTGAGGTTTCAGCCAAGAACTGGACAACTGCTGGCAGCAGCCTCAGATAAAATGGTATCCATCTTTGATGTTGAAACTGACAGGCAAACGCACTCGTTCCAGGGGCACTCTGACATGGTAAACTACATTTGTTGGAATGCAAATGGAGAATATCTGGCATCTGTCAGTCAGAACTTGGTGAAGATTTGGTCAATATCTTCGGGAGAGTGTATTCAGGAGCTCGGCTCTAACGGCAACCAGTTTCATTCGTGTGTTTTTCACCCAAGTTATTCAAATCTCTTGGTCATCGGAGGAAACTCGTCTTTGGAGCTGTGGAACATGGCTGAGAACAAGAGCATGACAGTGGCGGCACACGAGAATATAATCTCGGCTCTGGCTCAGTCACCTGACACTGGAATGGTTGCGTCGGCAAGTCATGACAGCTACGTCAAGTTATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

775

Amino Acids

84.65

Weight (kDa)

7.33

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 478 - 598 1.3e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 486 - 590 6e-13 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 489 - 690 8.6e-16 MABP1/WDR62 second WD40 domain
EIF3I PF24805 492 - 570 1.1e-06 EIF3I
WD40_Prp19 PF24814 495 - 767 5.4e-41 Prp19 WD40 domain
WD40_Gbeta PF25391 495 - 612 5.4e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 495 - 620 1.5e-26 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 496 - 571 5.7e-11 WDHD1 first WD40 domain
WD40 PF00400 497 - 525 4.6e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 497 - 650 1e-16 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 497 - 617 3.6e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 497 - 612 6.3e-21 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 497 - 570 3.1e-15 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 498 - 593 2.9e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 534 - 567 6.2e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 541 - 773 2e-23 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 542 - 723 2.1e-20 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 598 - 733 2.3e-18 WDR3 first beta-propeller domain
B-prop_COPA_B_2nd PF04053 600 - 709 2.6e-06 COPA/B second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 603 - 699 5.9e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 605 - 770 1.2e-10 WDR36/Utp21 first beta-propeller
Beta-prop_EML_2 PF23414 619 - 699 2.5e-07 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 622 - 741 5.2e-07 WDR55
Beta-prop_WDR5 PF25175 622 - 773 2.5e-27 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 625 - 773 3.4e-23 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 628 - 771 6.6e-06 WDR19 first beta-propeller
Beta-prop_EML PF23409 634 - 739 2.2e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 635 - 754 6.1e-08 G protein beta WD-40 repeat protein
WD40 PF00400 656 - 691 6e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 668 - 774 1.5e-11 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2306
Acc36I ACCTGC 1 cut(s) 1135
AccB1I GGYRCC 1 cut(s) 850
AccB7I CCANNNNNTGG 1 cut(s) 1901
AciI CCGC 1 cut(s) 2228
AclWI GGATC 9 cut(s) 116, 326, 339, 656, 669, 902, 915, 988, 1401
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 4 cut(s) 594, 793, 1648, 1818
AcuI CTGAAG 3 cut(s) 809, 1503, 1656
AdeI CACNNNGTG 1 cut(s) 1199
AfaI GTAC 4 cut(s) 543, 804, 1826, 1852
AfiI CCNNNNNNNGG 8 cut(s) 350, 755, 770, 797, 938, 1202, 1901, 2273
AgsI TTSAA 9 cut(s) 64, 161, 727, 754, 946, 1319, 1863, 1955, 2154
AhdI GACNNNNNGTC 1 cut(s) 1266
AjnI CCWGG 4 cut(s) 142, 565, 1195, 1980
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AloI GAACNNNNNNTCC 2 cut(s) 1270, 1302
Alw21I GWGCWC 3 cut(s) 316, 1601, 2103
Alw26I GTCTC 1 cut(s) 1377
AlwI GGATC 9 cut(s) 116, 326, 339, 656, 669, 902, 915, 988, 1401
AlwNI CAGNNNCTG 2 cut(s) 221, 289
AoxI GGCC 5 cut(s) 402, 501, 532, 761, 1159
ApoI RAATTY 4 cut(s) 594, 793, 1648, 1818
ArsI GACNNNNNNTTYG 2 cut(s) 1548, 1580
AspA2I CCTAGG 1 cut(s) 932
AspLEI GCGC 3 cut(s) 7, 309, 481
AspS9I GGNCC 5 cut(s) 502, 1124, 1160, 1268, 1482
AsuHPI GGTGA 8 cut(s) 1002, 1158, 1211, 1475, 1740, 2071, 2132, 2256
AsuNHI GCTAGC 1 cut(s) 412
AvaII GGWCC 3 cut(s) 1124, 1268, 1482
AvrII CCTAGG 1 cut(s) 932
BaeGI GKGCMC 2 cut(s) 1743, 1989
BamHI GGATCC 3 cut(s) 331, 661, 907
BanI GGYRCC 1 cut(s) 850
BanII GRGCYC 1 cut(s) 2103
BauI CACGAG 1 cut(s) 2234
BbsI GAAGAC 3 cut(s) 737, 1353, 1787
Bbv12I GWGCWC 3 cut(s) 316, 1601, 2103
BccI CCATC 8 cut(s) 406, 493, 950, 971, 1196, 1370, 1517, 1949
BceAI ACGGC 1 cut(s) 2128
BciT130I CCWGG 4 cut(s) 144, 567, 1197, 1982
BciVI GTATCC 2 cut(s) 1573, 1948
BclI TGATCA 1 cut(s) 1804
BcoDI GTCTC 1 cut(s) 1377
BfaI CTAG 4 cut(s) 336, 413, 933, 1539
BfmI CTRYAG 5 cut(s) 117, 225, 290, 717, 1586
BfuAI ACCTGC 1 cut(s) 1135
BfuI GTATCC 2 cut(s) 1573, 1948
BlnI CCTAGG 1 cut(s) 932
Bme1390I CCNGG 4 cut(s) 144, 567, 1197, 1982
Bme18I GGWCC 3 cut(s) 1124, 1268, 1482
BmeRI GACNNNNNGTC 1 cut(s) 1266
BmgT120I GGNCC 5 cut(s) 502, 1124, 1160, 1268, 1482
BmiI GGNNCC 4 cut(s) 333, 663, 852, 909
BmrFI CCNGG 4 cut(s) 144, 567, 1197, 1982
BmrI ACTGGG 2 cut(s) 25, 1164
BmsI GCATC 7 cut(s) 21, 127, 478, 1023, 1190, 1690, 2048
BmtI GCTAGC 1 cut(s) 416
BmuI ACTGGG 2 cut(s) 25, 1164
BoxI GACNNNNGTC 1 cut(s) 1288
BpiI GAAGAC 3 cut(s) 737, 1353, 1787
BplI GAGNNNNNCTC 4 cut(s) 138, 170, 2090, 2122
BpmI CTGGAG 1 cut(s) 978
Bpu10I CCTNAGC 1 cut(s) 631
BsaAI YACGTR 1 cut(s) 1727
BsaBI GATNNNNATC 1 cut(s) 906
BsaJI CCNNGG 2 cut(s) 932, 1981
Bsc4I CCNNNNNNNGG 8 cut(s) 350, 755, 770, 797, 938, 1202, 1901, 2273
Bse118I RCCGGY 1 cut(s) 1139
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 1 cut(s) 906
BseBI CCWGG 4 cut(s) 144, 567, 1197, 1982
BseDI CCNNGG 2 cut(s) 932, 1981
BseGI GGATG 4 cut(s) 340, 504, 1315, 1705
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 8 cut(s) 350, 755, 770, 797, 938, 1202, 1901, 2273
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 8 cut(s) 329, 369, 656, 1043, 1670, 1938, 2196, 2273
BseRI GAGGAG 1 cut(s) 336
BseSI GKGCMC 2 cut(s) 1743, 1989
BsgI GTGCAG 1 cut(s) 1860
Bsh1285I CGRYCG 1 cut(s) 791
BshFI GGCC 5 cut(s) 404, 503, 534, 763, 1161
BshNI GGYRCC 1 cut(s) 850
BsiEI CGRYCG 1 cut(s) 791
BsiHKAI GWGCWC 3 cut(s) 316, 1601, 2103
BsiSI CCGG 1 cut(s) 1140
BslFI GGGAC 3 cut(s) 194, 1417, 1744
BslI CCNNNNNNNGG 8 cut(s) 350, 755, 770, 797, 938, 1202, 1901, 2273
BsmAI GTCTC 1 cut(s) 1377
BsmFI GGGAC 3 cut(s) 194, 1417, 1744
BsmI GAATGC 3 cut(s) 211, 952, 2023
BsnI GGCC 5 cut(s) 404, 503, 534, 763, 1161
Bsp1286I GDGCHC 5 cut(s) 316, 1601, 1743, 1989, 2103
Bsp1407I TGTACA 1 cut(s) 802
Bsp143I GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
BspACI CCGC 1 cut(s) 2228
BspANI GGCC 5 cut(s) 404, 503, 534, 763, 1161
BspCNI CTCAG 8 cut(s) 328, 370, 655, 1042, 1669, 1937, 2197, 2272
BspHI TCATGA 3 cut(s) 49, 1807, 2296
BspLI GGNNCC 4 cut(s) 333, 663, 852, 909
BspMAI CTGCAG 4 cut(s) 229, 294, 721, 1590
BspMI ACCTGC 1 cut(s) 1135
BspOI GCTAGC 1 cut(s) 416
BspPI GGATC 9 cut(s) 116, 326, 339, 656, 669, 902, 915, 988, 1401
BspT107I GGYRCC 1 cut(s) 850
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 1139
BsrGI TGTACA 1 cut(s) 802
BssAI RCCGGY 1 cut(s) 1139
BssECI CCNNGG 2 cut(s) 932, 1981
BssMI GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
BssSI CACGAG 1 cut(s) 2234
BssT1I CCWWGG 1 cut(s) 932
Bst2BI CACGAG 1 cut(s) 2234
Bst2UI CCWGG 4 cut(s) 144, 567, 1197, 1982
Bst4CI ACNGT 4 cut(s) 696, 1156, 1241, 2224
Bst6I CTCTTC 2 cut(s) 1120, 1522
BstAPI GCANNNNNTGC 3 cut(s) 137, 289, 298
BstAUI TGTACA 1 cut(s) 802
BstBAI YACGTR 1 cut(s) 1727
BstC8I GCNNGC 6 cut(s) 78, 414, 1179, 1536, 1662, 1914
BstF5I GGATG 4 cut(s) 340, 504, 1315, 1705
BstHHI GCGC 3 cut(s) 7, 309, 481
BstKTI GATC 8 cut(s) 124, 334, 436, 664, 910, 983, 1396, 1807
BstMAI GTCTC 1 cut(s) 1377
BstMBI GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
BstMCI CGRYCG 1 cut(s) 791
BstNI CCWGG 4 cut(s) 144, 567, 1197, 1982
BstNSI RCATGY 1 cut(s) 565
BstPAI GACNNNNGTC 1 cut(s) 1288
BstSCI CCNGG 4 cut(s) 142, 565, 1195, 1980
BstSFI CTRYAG 5 cut(s) 117, 225, 290, 717, 1586
BstSLI GKGCMC 2 cut(s) 1743, 1989
BstV2I GAAGAC 3 cut(s) 737, 1353, 1787
BstX2I RGATCY 5 cut(s) 121, 331, 661, 907, 1393
BstXI CCANNNNNNTGG 2 cut(s) 499, 1306
BstYI RGATCY 5 cut(s) 121, 331, 661, 907, 1393
BsuI GTATCC 2 cut(s) 1573, 1948
BsuRI GGCC 5 cut(s) 404, 503, 534, 763, 1161
BtsCI GGATG 4 cut(s) 340, 504, 1315, 1705
BtsI GCAGTG 2 cut(s) 316, 714
BtsIMutI CAGTG 7 cut(s) 316, 412, 606, 714, 1152, 2229, 2271
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 6 cut(s) 78, 414, 1179, 1536, 1662, 1914
CaiI CAGNNNCTG 2 cut(s) 221, 289
CciI TCATGA 3 cut(s) 49, 1807, 2296
CfoI GCGC 3 cut(s) 7, 309, 481
Cfr10I RCCGGY 1 cut(s) 1139
Cfr13I GGNCC 5 cut(s) 502, 1124, 1160, 1268, 1482
CseI GACGC 1 cut(s) 2274
CsiI ACCWGGT 1 cut(s) 1195
Csp6I GTAC 4 cut(s) 542, 803, 1825, 1851
CviQI GTAC 4 cut(s) 542, 803, 1825, 1851
DpnI GATC 8 cut(s) 123, 333, 435, 663, 909, 982, 1395, 1806
DpnII GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
DraIII CACNNNGTG 1 cut(s) 1199
DrdI GACNNNNNNGTC 1 cut(s) 2306
DriI GACNNNNNGTC 1 cut(s) 1266
DseDI GACNNNNNNGTC 1 cut(s) 2306
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 1120, 1522
Eam1105I GACNNNNNGTC 1 cut(s) 1266
EarI CTCTTC 2 cut(s) 1120, 1522
Ecl136II GAGCTC 1 cut(s) 2101
Eco130I CCWWGG 1 cut(s) 932
Eco24I GRGCYC 1 cut(s) 2103
Eco47I GGWCC 3 cut(s) 1124, 1268, 1482
Eco53kI GAGCTC 1 cut(s) 2101
Eco57I CTGAAG 3 cut(s) 809, 1503, 1656
EcoICRI GAGCTC 1 cut(s) 2101
EcoRI GAATTC 1 cut(s) 594
EcoRII CCWGG 4 cut(s) 142, 565, 1195, 1980
EcoT14I CCWWGG 1 cut(s) 932
EcoT38I GRGCYC 1 cut(s) 2103
ErhI CCWWGG 1 cut(s) 932
FaqI GGGAC 3 cut(s) 194, 1417, 1744
FbaI TGATCA 1 cut(s) 1804
FokI GGATG 4 cut(s) 327, 511, 1322, 1712
FriOI GRGCYC 1 cut(s) 2103
FspBI CTAG 4 cut(s) 336, 413, 933, 1539
GlaI GCGC 3 cut(s) 6, 308, 480
GsuI CTGGAG 1 cut(s) 978
HaeIII GGCC 5 cut(s) 404, 503, 534, 763, 1161
HapII CCGG 1 cut(s) 1140
HgaI GACGC 1 cut(s) 2274
HhaI GCGC 3 cut(s) 7, 309, 481
Hin6I GCGC 3 cut(s) 5, 307, 479
HinP1I GCGC 3 cut(s) 5, 307, 479
HincII GTYRAC 2 cut(s) 1237, 1678
HindII GTYRAC 2 cut(s) 1237, 1678
HindIII AAGCTT 1 cut(s) 1252
HinfI GANTC 6 cut(s) 461, 727, 774, 1358, 1417, 1439
HpaII CCGG 1 cut(s) 1140
HphI GGTGA 8 cut(s) 1002, 1158, 1211, 1475, 1740, 2071, 2132, 2256
Hpy166II GTNNAC 6 cut(s) 803, 1010, 1237, 1678, 1724, 2002
Hpy8I GTNNAC 6 cut(s) 803, 1010, 1237, 1678, 1724, 2002
Hpy99I CGWCG 1 cut(s) 2290
HpyAV CCTTC 8 cut(s) 98, 103, 321, 675, 748, 1053, 1173, 1268
HpyCH4III ACNGT 4 cut(s) 696, 1156, 1241, 2224
HpyCH4IV ACGT 3 cut(s) 1262, 1726, 2307
HpySE526I ACGT 3 cut(s) 1262, 1726, 2307
HspAI GCGC 3 cut(s) 5, 307, 479
Ksp22I TGATCA 1 cut(s) 1804
Kzo9I GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
LmnI GCTCC 3 cut(s) 262, 2098, 2188
LweI GCATC 7 cut(s) 21, 127, 478, 1023, 1190, 1690, 2048
MabI ACCWGGT 1 cut(s) 1195
MaeI CTAG 4 cut(s) 336, 413, 933, 1539
MaeII ACGT 3 cut(s) 1262, 1726, 2307
MaeIII GTNAC 7 cut(s) 11, 318, 783, 893, 1498, 1508, 2262
MalI GATC 8 cut(s) 123, 333, 435, 663, 909, 982, 1395, 1806
MboI GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
MfeI CAATTG 2 cut(s) 132, 278
MflI RGATCY 5 cut(s) 121, 331, 661, 907, 1393
MhlI GDGCHC 5 cut(s) 316, 1601, 1743, 1989, 2103
MlyI GAGTC 1 cut(s) 1426
MmeI TCCRAC 1 cut(s) 1994
MseI TTAA 5 cut(s) 252, 602, 1209, 1409, 1836
MslI CAYNNNNRTG 5 cut(s) 422, 1812, 2130, 2222, 2276
MspA1I CMGCKG 4 cut(s) 289, 295, 518, 1660
MspI CCGG 1 cut(s) 1140
MspR9I CCNGG 4 cut(s) 144, 567, 1197, 1982
MunI CAATTG 2 cut(s) 132, 278
Mva1269I GAATGC 3 cut(s) 211, 952, 2023
MvaI CCWGG 4 cut(s) 144, 567, 1197, 1982
NdeII GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
NheI GCTAGC 1 cut(s) 412
NlaIV GGNNCC 4 cut(s) 333, 663, 852, 909
NmeAIII GCCGAG 2 cut(s) 2082, 2228
NmuCI GTSAC 2 cut(s) 1508, 2262
NspI RCATGY 1 cut(s) 565
PagI TCATGA 3 cut(s) 49, 1807, 2296
PctI GAATGC 3 cut(s) 211, 952, 2023
PfeI GAWTC 5 cut(s) 461, 727, 774, 1358, 1439
PflMI CCANNNNNTGG 1 cut(s) 1901
PleI GAGTC 1 cut(s) 1425
PpsI GAGTC 1 cut(s) 1425
Ppu21I YACGTR 1 cut(s) 1727
PshAI GACNNNNGTC 1 cut(s) 1288
Psp124BI GAGCTC 1 cut(s) 2103
Psp6I CCWGG 4 cut(s) 142, 565, 1195, 1980
PspGI CCWGG 4 cut(s) 142, 565, 1195, 1980
PspN4I GGNNCC 4 cut(s) 333, 663, 852, 909
PspPI GGNCC 5 cut(s) 502, 1124, 1160, 1268, 1482
PsrI GAACNNNNNNTAC 2 cut(s) 1843, 1875
PstI CTGCAG 4 cut(s) 229, 294, 721, 1590
PstNI CAGNNNCTG 2 cut(s) 221, 289
PsuI RGATCY 5 cut(s) 121, 331, 661, 907, 1393
PvuII CAGCTG 4 cut(s) 289, 295, 518, 1660
RsaI GTAC 4 cut(s) 543, 804, 1826, 1852
RsaNI GTAC 4 cut(s) 542, 803, 1825, 1851
RseI CAYNNNNRTG 5 cut(s) 422, 1812, 2130, 2222, 2276
SacI GAGCTC 1 cut(s) 2103
SaqAI TTAA 5 cut(s) 252, 602, 1209, 1409, 1836
Sau3AI GATC 8 cut(s) 121, 331, 433, 661, 907, 980, 1393, 1804
Sau96I GGNCC 5 cut(s) 502, 1124, 1160, 1268, 1482
SchI GAGTC 1 cut(s) 1426
ScrFI CCNGG 4 cut(s) 144, 567, 1197, 1982
SduI GDGCHC 5 cut(s) 316, 1601, 1743, 1989, 2103
SexAI ACCWGGT 1 cut(s) 1195
SfaNI GCATC 7 cut(s) 21, 127, 478, 1023, 1190, 1690, 2048
SfcI CTRYAG 5 cut(s) 117, 225, 290, 717, 1586
SinI GGWCC 3 cut(s) 1124, 1268, 1482
SmiMI CAYNNNNRTG 5 cut(s) 422, 1812, 2130, 2222, 2276
SsiI CCGC 1 cut(s) 2228
SspI AATATT 1 cut(s) 1834
SspMI CTAG 4 cut(s) 336, 413, 933, 1539
SstI GAGCTC 1 cut(s) 2103
StyD4I CCNGG 4 cut(s) 142, 565, 1195, 1980
StyI CCWWGG 1 cut(s) 932
TaaI ACNGT 4 cut(s) 696, 1156, 1241, 2224
TaiI ACGT 3 cut(s) 1265, 1729, 2310
TaqI TCGA 1 cut(s) 1004
TaqII GACCGA 1 cut(s) 805
TatI WGTACW 2 cut(s) 802, 1850
TauI GCSGC 1 cut(s) 2231
TfiI GAWTC 5 cut(s) 461, 727, 774, 1358, 1439
Tru1I TTAA 5 cut(s) 252, 602, 1209, 1409, 1836
Tru9I TTAA 5 cut(s) 252, 602, 1209, 1409, 1836
TscAI CASTG 7 cut(s) 316, 412, 613, 721, 1159, 2229, 2278
TseFI GTSAC 2 cut(s) 1508, 2262
Tsp45I GTSAC 2 cut(s) 1508, 2262
TspGWI ACGGA 4 cut(s) 116, 912, 1278, 1495
TspRI CASTG 7 cut(s) 316, 412, 613, 721, 1159, 2229, 2278
Van91I CCANNNNNTGG 1 cut(s) 1901
VpaK11BI GGWCC 3 cut(s) 1124, 1268, 1482
XapI RAATTY 4 cut(s) 594, 793, 1648, 1818
XceI RCATGY 1 cut(s) 565
XmaJI CCTAGG 1 cut(s) 932
XspI CTAG 4 cut(s) 336, 413, 933, 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.