MD04G1005500.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001113
Physical Location & Seq
Forward (+)
649341 .. 657667
8327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1005500.v1.1.491

Sequence Viewer

Length: 2316 bp
ATGGCGCAGAGTAGTTGGGAAGCAGATAAGATGCTTGATGTATACATATATGATTATTTAGTGAAGAAAGAATTACATGCCACTGCTAAGTCTTTCATGACCGAAGGGAAGGTTGCACCCGATCCAGTAGCCATTGATGCTCCCGGGGGCTTTCTTTTTGAATGGTGGTCTGTCTTTTGGGACATTTTTATTGCGAGGACAAACGATAAACATTCTGAGGCTGCTGCAGCGTATATAGAGGTACAACAAAGTAAAGCAAAAGAGCAGCAACAGCTGCAAATGCAGCAGTTGCATCTAATGCGTCAAGCTCAGATGCAACGGAGAGATCCTAATCATCCTCCCCTTGGTGGTCCACTAAATTCAATTAGTTCTGAAGGGATGCTTGGGCAATCTACTACTAGTGCACTGGCGGCAAAAATGTACGAGGACCGCTTGAAACACCCCAATCCAATTGAGTCAGAGACATCCCAACCACTCCTTGATGCCAGAGTTGCCCTTCTAAGATCAACGAACCATCCTGGTCAGATGGTTCAAGGAAACCCTGGAAGTGTTAATGCAGCATTACAACAAATCCAGGCTCGAAGTCAACAGGCCACAGACATAAAGAGGGAGGTTAACATGGGCACTGCTCAGAGATCTTTGCCTATGGATTCTTCAATTTATGGGCAGGGAATGGTTCAGTCAAAACCTGGATTAGGAAATGCAGGATTGAACCCAGGAGTTGGTGGTCTTCCATTGAAGGGGTGGCCTTTAACTGGAATTGATCAAATGAGGCCTGGTTTAGGTGGACAAGTTCAAAAGCCTTTCCTGCAAGGTGCAACTCAGTTTCAGGTTCTCTCACCGCAACAACAACAGCTTTTGGCACAGGCTCAGGGACAAGGAAATGGTGGTAGCTCATCAATTTATGGAGATATGAGGGGATTTTCTAGGGGAAATTTAAATACAAAAGATACCCAACAAATGACAAATAATGGCTCCATAGGTTCTCCTATGCAGTCAAATTCATCAAAGATGCAACACTCCTCATCTCAACAACAGGATCCTTTGCAGTCTCAGCAAGTACAGCAGAATAACCGCAAAAGGAAAGGACCTTCATCCTCAGGACAGCCTGCTAACAGTACTGGTACAGGAAATACAATTGGTCCTTCGCCCAATTCTCAACCATCAACTCCATCCACCCATACCCCTGGAGACGGAGTTGCTATGACTACTAATTTGCCAAATTCGAGCAGTGTTCCAAAAAGTATGATGATGTACGGTGCAGATGGAACAGGTGGCCTTGCATCATCCACAAACCAGCTGGAAGACATTGAACAATTTGGGGATGTTGGCTCTTTAGAAGATAATGTGGAATCATTCTTGTCACATGATGATGGAGATGGTAGGGATTTATTTGGCACATTGAAACGGAACCCTGCACATTCTGCAGAAGCTTCAAAGGGTTTTTCCTTCAGTGAAGTTGGTTCAATACGTAAAAGTAATACCAAAGTTGTCTGTTGCCACTTCTCTTCAGATGGAAAATTGCTAGCCAGTGCTGGCCATGACAAAAAGGTTGTTGTTTGGAATATGGAAACTCTACAAACTGAATTCACACCAGAGGAGCATGGCCTGATAATTACAGATGTTCGATTTAGACCAAATTCATCTCAGTTGGCAACTTCTTCATTTGACACAACTGTGCGACTTTGGGATGCTGCTGAACCAAACTATTGTTTACAGACATATGCTGGGCATACCTCACATGTAACATCACTTGATTTCCACCCTAAGAAGAATGACCTTTTCTGCTCTTGTGATGCCAACAATGAGATTCGCTATTGGAATATCAATCAGTATGCTAGCACTCGCGTTTCTAAGGGAGGTTCAGCACAGGTGAGATTCCAACCGAGAATTGGACAGTTGCTGGCTGCAGCATCCGGGAATGTTGTATCTATATTTGATGCTGAGACCGACAGGCAGACACATTCATTGCCGGGCCACTCCACGGAGGTACACTCTCTTTGCTGGGATACAAATGGAGAGTATTTAGCATCTGCCAGTCAAGAGTCTGTCAGAGTGTGGTCATTATCCTCTGGAGAGTGCATTCATGAGCTCAGTTCTAGTGGAAACATGTTCCATTCTATTGTATTCCATCCAAGCTACTCGACTCTCTTGGTTATTGGAGGATACCAGTCATTTGAGCTCTGGAACATGTCTGAGAACAAGTGTATGACAATTCAGGCACATGAGTGTGTAATATCAGCTTTGGCACAGTCACCGGTTACAGGAATGGTCGCCTCCGCCAGTTTCGACAAGTCCGTTAAAATCTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

772

Amino Acids

83.66

Weight (kDa)

6.15

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 10 - 36 1.4e-06 LisH
Beta-prop_TEP1_2nd PF25047 483 - 586 3.6e-11 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 484 - 568 2.3e-11 WDHD1 first WD40 domain
WDR55 PF24796 486 - 613 9.9e-08 WDR55
Beta-prop_CAF1B_HIR1 PF24105 487 - 613 1.3e-06 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 492 - 730 1.2e-14 MABP1/WDR62 second WD40 domain
Beta-prop_WDR5 PF25175 492 - 616 1.2e-25 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 492 - 613 3.9e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 493 - 618 4.9e-12 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 493 - 594 2.2e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 493 - 522 6.9e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 494 - 568 4.3e-14 THOC3 beta-propeller domain
WD40_Prp19 PF24814 494 - 764 3.2e-39 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 494 - 609 4.3e-22 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 496 - 599 3.3e-10 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 497 - 679 5.4e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 507 - 606 1.7e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 532 - 564 8.9e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 533 - 680 1.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 539 - 771 1.4e-36 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 573 - 719 3e-15 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 578 - 713 2e-09 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 580 - 738 2.7e-07 WDR55
Beta-prop_WDR3_1st PF25173 596 - 771 1.9e-20 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 604 - 764 9e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 620 - 770 2.5e-24 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 632 - 770 1.7e-12 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 634 - 751 8.9e-06 G protein beta WD-40 repeat protein
WD40 PF00400 653 - 688 8.2e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 663 - 771 4.7e-10 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 722
AccI GTMKAC 1 cut(s) 42
AccII CGCG 1 cut(s) 1848
AciI CCGC 5 cut(s) 410, 430, 842, 1075, 2280
AclWI GGATC 4 cut(s) 116, 320, 1034, 1047
AcoI YGGCCR 1 cut(s) 1537
AcsI RAATTY 6 cut(s) 358, 934, 1000, 1222, 1586, 1639
AcuI CTGAAG 3 cut(s) 393, 1435, 1494
AfaI GTAC 7 cut(s) 243, 422, 1062, 1120, 1126, 1256, 1992
AflIII ACRYGT 3 cut(s) 1741, 2109, 2190
AgeI ACCGGT 1 cut(s) 2257
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 7 cut(s) 517, 541, 573, 688, 715, 775, 1186
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AleI CACNNNNGTG 2 cut(s) 1676, 2227
Alw21I GWGCWC 3 cut(s) 406, 2094, 2184
Alw26I GTCTC 4 cut(s) 455, 1056, 1185, 1940
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 4 cut(s) 116, 320, 1034, 1047
AlwNI CAGNNNCTG 1 cut(s) 1903
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 7 cut(s) 591, 746, 773, 1276, 1537, 1606, 1975
ApaLI GTGCAC 1 cut(s) 402
ApoI RAATTY 6 cut(s) 358, 934, 1000, 1222, 1586, 1639
ArsI GACNNNNNNTTYG 2 cut(s) 1198, 1230
AsiGI ACCGGT 1 cut(s) 2257
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 5 cut(s) 350, 427, 1088, 1142, 1975
AsuC2I CCSGG 4 cut(s) 144, 145, 1918, 1974
AsuHPI GGTGA 3 cut(s) 831, 1885, 2247
AsuNHI GCTAGC 2 cut(s) 1525, 1838
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 4 cut(s) 350, 427, 1088, 1142
AxyI CCTNAGG 1 cut(s) 1099
BaeGI GKGCMC 2 cut(s) 406, 626
BalI TGGCCA 1 cut(s) 1539
BamHI GGATCC 1 cut(s) 1039
BanII GRGCYC 2 cut(s) 2094, 2184
BbsI GAAGAC 2 cut(s) 722, 1311
Bbv12I GWGCWC 3 cut(s) 406, 2094, 2184
BccI CCATC 9 cut(s) 520, 522, 1171, 1180, 1259, 1367, 1373, 1508, 2139
BciT130I CCWGG 7 cut(s) 519, 543, 575, 690, 717, 777, 1188
BciVI GTATCC 2 cut(s) 2002, 2159
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 4 cut(s) 144, 145, 1918, 1974
BcoDI GTCTC 4 cut(s) 455, 1056, 1185, 1940
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 5 cut(s) 399, 927, 1526, 1839, 2100
BfmI CTRYAG 3 cut(s) 225, 1425, 1908
BfuI GTATCC 2 cut(s) 2002, 2159
BglII AGATCT 1 cut(s) 635
BmcAI AGTACT 1 cut(s) 1120
Bme18I GGWCC 4 cut(s) 350, 427, 1088, 1142
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 5 cut(s) 350, 427, 1088, 1142, 1975
BmiI GGNNCC 3 cut(s) 976, 1041, 1412
BmtI GCTAGC 2 cut(s) 1529, 1842
BpiI GAAGAC 2 cut(s) 722, 1311
BplI GAGNNNNNCTC 2 cut(s) 1979, 2011
BpmI CTGGAG 2 cut(s) 1209, 2094
Bpu10I CCTNAGC 1 cut(s) 870
BpuMI CCSGG 4 cut(s) 144, 145, 1918, 1974
BsaAI YACGTR 1 cut(s) 1472
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 1940
BsaJI CCNNGG 7 cut(s) 143, 144, 343, 541, 715, 1186, 1983
BsaWI WCCGGW 1 cut(s) 2257
BsaXI ACNNNNNCTCC 2 cut(s) 1182, 1212
Bse118I RCCGGY 1 cut(s) 2257
Bse1I ACTGG 8 cut(s) 125, 411, 760, 1126, 1530, 2037, 2170, 2283
Bse21I CCTNAGG 1 cut(s) 1099
Bse3DI GCAATG 1 cut(s) 1967
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 7 cut(s) 519, 543, 575, 690, 717, 777, 1188
BseDI CCNNGG 7 cut(s) 143, 144, 343, 541, 715, 1186, 1983
BseJI GATNNNNATC 1 cut(s) 330
BseMI GCAATG 1 cut(s) 1967
BseNI ACTGG 8 cut(s) 125, 411, 760, 1126, 1530, 2037, 2170, 2283
BseRI GAGGAG 2 cut(s) 1012, 1613
BseSI GKGCMC 2 cut(s) 406, 626
BseYI CCCAGC 2 cut(s) 1727, 2004
BsgI GTGCAG 2 cut(s) 1281, 1401
Bsh1236I CGCG 1 cut(s) 1848
BshFI GGCC 7 cut(s) 593, 748, 775, 1278, 1539, 1608, 1977
BshTI ACCGGT 1 cut(s) 2257
BsiHKAI GWGCWC 3 cut(s) 406, 2094, 2184
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 4 cut(s) 144, 1917, 1973, 2258
BslFI GGGAC 2 cut(s) 194, 888
BsmAI GTCTC 4 cut(s) 455, 1056, 1185, 1940
BsmBI CGTCTC 1 cut(s) 1185
BsmFI GGGAC 2 cut(s) 194, 888
BsmI GAATGC 1 cut(s) 2082
BsnI GGCC 7 cut(s) 593, 748, 775, 1278, 1539, 1608, 1977
Bso31I GGTCTC 1 cut(s) 1940
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 4 cut(s) 406, 626, 2094, 2184
Bsp143I GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
BspACI CCGC 5 cut(s) 410, 430, 842, 1075, 2280
BspANI GGCC 7 cut(s) 593, 748, 775, 1278, 1539, 1608, 1977
BspFNI CGCG 1 cut(s) 1848
BspHI TCATGA 2 cut(s) 96, 2086
BspLI GGNNCC 3 cut(s) 976, 1041, 1412
BspMAI CTGCAG 3 cut(s) 229, 1429, 1912
BspOI GCTAGC 2 cut(s) 1529, 1842
BspPI GGATC 4 cut(s) 116, 320, 1034, 1047
BspTNI GGTCTC 1 cut(s) 1940
BsrDI GCAATG 1 cut(s) 1967
BsrFI RCCGGY 1 cut(s) 2257
BsrI ACTGG 8 cut(s) 125, 411, 760, 1126, 1530, 2037, 2170, 2283
BssAI RCCGGY 1 cut(s) 2257
BssECI CCNNGG 7 cut(s) 143, 144, 343, 541, 715, 1186, 1983
BssMI GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
BssNAI GTATAC 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 343
Bst1107I GTATAC 1 cut(s) 43
Bst2UI CCWGG 7 cut(s) 519, 543, 575, 690, 717, 777, 1188
Bst4CI ACNGT 5 cut(s) 1118, 1259, 1678, 1899, 2253
Bst6I CTCTTC 1 cut(s) 1513
BstAPI GCANNNNNTGC 4 cut(s) 274, 289, 298, 1424
BstBAI YACGTR 1 cut(s) 1472
BstC8I GCNNGC 5 cut(s) 1110, 1527, 1537, 1840, 1905
BstDSI CCRYGG 1 cut(s) 1983
BstENI CCTNNNNNAGG 2 cut(s) 693, 780
BstFNI CGCG 1 cut(s) 1848
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 6 cut(s) 124, 328, 506, 638, 766, 1042
BstMAI GTCTC 4 cut(s) 455, 1056, 1185, 1940
BstMBI GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
BstNI CCWGG 7 cut(s) 519, 543, 575, 690, 717, 777, 1188
BstNSI RCATGY 4 cut(s) 80, 1745, 2113, 2194
BstSFI CTRYAG 3 cut(s) 225, 1425, 1908
BstSLI GKGCMC 2 cut(s) 406, 626
BstSNI TACGTA 1 cut(s) 1472
BstUI CGCG 1 cut(s) 1848
BstV2I GAAGAC 2 cut(s) 722, 1311
BstX2I RGATCY 3 cut(s) 325, 635, 1039
BstXI CCANNNNNNTGG 1 cut(s) 1187
BstYI RGATCY 3 cut(s) 325, 635, 1039
BstZ17I GTATAC 1 cut(s) 43
Bsu36I CCTNAGG 1 cut(s) 1099
BsuI GTATCC 2 cut(s) 2002, 2159
BsuRI GGCC 7 cut(s) 593, 748, 775, 1278, 1539, 1608, 1977
BtgI CCRYGG 1 cut(s) 1983
BtsI GCAGTG 3 cut(s) 81, 624, 1237
BtsIMutI CAGTG 6 cut(s) 81, 404, 624, 1237, 1459, 1537
Cac8I GCNNGC 5 cut(s) 1110, 1527, 1537, 1840, 1905
CaiI CAGNNNCTG 1 cut(s) 1903
CciI TCATGA 2 cut(s) 96, 2086
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 2257
Cfr13I GGNCC 5 cut(s) 350, 427, 1088, 1142, 1975
Cfr9I CCCGGG 1 cut(s) 143
CseI GACGC 1 cut(s) 290
Csp6I GTAC 7 cut(s) 242, 421, 1061, 1119, 1125, 1255, 1991
CspAI ACCGGT 1 cut(s) 2257
CviQI GTAC 7 cut(s) 242, 421, 1061, 1119, 1125, 1255, 1991
DpnI GATC 6 cut(s) 123, 327, 505, 637, 765, 1041
DpnII GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
DraI TTTAAA 1 cut(s) 939
EaeI YGGCCR 1 cut(s) 1537
Eam1104I CTCTTC 1 cut(s) 1513
EarI CTCTTC 1 cut(s) 1513
EciI GGCGGA 1 cut(s) 2269
Ecl136II GAGCTC 2 cut(s) 2092, 2182
Eco105I TACGTA 1 cut(s) 1472
Eco130I CCWWGG 1 cut(s) 343
Eco147I AGGCCT 1 cut(s) 775
Eco24I GRGCYC 2 cut(s) 2094, 2184
Eco31I GGTCTC 1 cut(s) 1940
Eco47I GGWCC 4 cut(s) 350, 427, 1088, 1142
Eco53kI GAGCTC 2 cut(s) 2092, 2182
Eco57I CTGAAG 3 cut(s) 393, 1435, 1494
Eco81I CCTNAGG 1 cut(s) 1099
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 2 cut(s) 2092, 2182
EcoNI CCTNNNNNAGG 2 cut(s) 693, 780
EcoO109I RGGNCCY 1 cut(s) 1088
EcoRI GAATTC 1 cut(s) 1586
EcoRII CCWGG 7 cut(s) 517, 541, 573, 688, 715, 775, 1186
EcoT14I CCWWGG 1 cut(s) 343
EcoT38I GRGCYC 2 cut(s) 2094, 2184
ErhI CCWWGG 1 cut(s) 343
Esp3I CGTCTC 1 cut(s) 1185
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 2 cut(s) 194, 888
FauNDI CATATG 1 cut(s) 1723
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 1 cut(s) 42
FriOI GRGCYC 2 cut(s) 2094, 2184
FspBI CTAG 5 cut(s) 399, 927, 1526, 1839, 2100
GlaI GCGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 1731, 2008
GsuI CTGGAG 2 cut(s) 1209, 2094
HaeIII GGCC 7 cut(s) 593, 748, 775, 1278, 1539, 1608, 1977
HapII CCGG 4 cut(s) 144, 1917, 1973, 2258
HgaI GACGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 7
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 587, 616
HindII GTYRAC 2 cut(s) 587, 616
HindIII AAGCTT 1 cut(s) 1431
HinfI GANTC 7 cut(s) 455, 650, 1352, 1810, 1878, 2045, 2146
HpaI GTTAAC 1 cut(s) 616
HpaII CCGG 4 cut(s) 144, 1917, 1973, 2258
HphI GGTGA 3 cut(s) 831, 1885, 2247
Hpy166II GTNNAC 8 cut(s) 43, 353, 404, 587, 616, 788, 1715, 1993
Hpy188I TCNGA 9 cut(s) 217, 312, 373, 460, 525, 633, 1513, 2054, 2197
Hpy188III TCNNGA 7 cut(s) 97, 1101, 2042, 2073, 2087, 2185, 2308
Hpy8I GTNNAC 8 cut(s) 43, 353, 404, 587, 616, 788, 1715, 1993
HpyAV CCTTC 8 cut(s) 98, 103, 368, 506, 733, 1101, 1155, 1459
HpyCH4III ACNGT 5 cut(s) 1118, 1259, 1678, 1899, 2253
HpyCH4IV ACGT 1 cut(s) 1471
HpySE526I ACGT 1 cut(s) 1471
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 763
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
LmnI GCTCC 3 cut(s) 145, 980, 1600
MaeI CTAG 5 cut(s) 399, 927, 1526, 1839, 2100
MaeII ACGT 1 cut(s) 1471
MaeIII GTNAC 4 cut(s) 1362, 1744, 2253, 2260
MalI GATC 6 cut(s) 123, 327, 505, 637, 765, 1041
MboI GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
MboII GAAGA 8 cut(s) 76, 645, 722, 1316, 1352, 1500, 1653, 1783
MfeI CAATTG 2 cut(s) 450, 1137
MflI RGATCY 3 cut(s) 325, 635, 1039
MhlI GDGCHC 4 cut(s) 406, 626, 2094, 2184
MlsI TGGCCA 1 cut(s) 1539
MluNI TGGCCA 1 cut(s) 1539
MlyI GAGTC 3 cut(s) 464, 2054, 2140
MmeI TCCRAC 1 cut(s) 1906
Mox20I TGGCCA 1 cut(s) 1539
MscI TGGCCA 1 cut(s) 1539
MseI TTAA 5 cut(s) 552, 615, 752, 938, 2301
MslI CAYNNNNRTG 4 cut(s) 1371, 1676, 2227, 2229
Msp20I TGGCCA 1 cut(s) 1539
MspA1I CMGCKG 2 cut(s) 274, 1300
MspI CCGG 4 cut(s) 144, 1917, 1973, 2258
MunI CAATTG 2 cut(s) 450, 1137
Mva1269I GAATGC 1 cut(s) 2082
MvaI CCWGG 7 cut(s) 519, 543, 575, 690, 717, 777, 1188
MvnI CGCG 1 cut(s) 1848
NciI CCSGG 4 cut(s) 144, 145, 1918, 1974
NdeI CATATG 1 cut(s) 1723
NdeII GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
NheI GCTAGC 2 cut(s) 1525, 1838
NlaIV GGNNCC 3 cut(s) 976, 1041, 1412
NmuCI GTSAC 2 cut(s) 1362, 2253
NspI RCATGY 4 cut(s) 80, 1745, 2113, 2194
OliI CACNNNNGTG 2 cut(s) 1676, 2227
PagI TCATGA 2 cut(s) 96, 2086
PceI AGGCCT 1 cut(s) 775
PciI ACATGT 3 cut(s) 1741, 2109, 2190
PcsI WCGNNNNNNNCGW 1 cut(s) 2295
PctI GAATGC 1 cut(s) 2082
PfeI GAWTC 4 cut(s) 650, 1352, 1810, 1878
PflMI CCANNNNNTGG 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 1916
PinAI ACCGGT 1 cut(s) 2257
PleI GAGTC 3 cut(s) 463, 2053, 2140
PpsI GAGTC 3 cut(s) 463, 2053, 2140
Ppu21I YACGTR 1 cut(s) 1472
PpuMI RGGWCCY 1 cut(s) 1088
PscI ACATGT 3 cut(s) 1741, 2109, 2190
Psp124BI GAGCTC 2 cut(s) 2094, 2184
Psp5II RGGWCCY 1 cut(s) 1088
Psp6I CCWGG 7 cut(s) 517, 541, 573, 688, 715, 775, 1186
PspFI CCCAGC 2 cut(s) 1727, 2004
PspGI CCWGG 7 cut(s) 517, 541, 573, 688, 715, 775, 1186
PspN4I GGNNCC 3 cut(s) 976, 1041, 1412
PspPI GGNCC 5 cut(s) 350, 427, 1088, 1142, 1975
PspPPI RGGWCCY 1 cut(s) 1088
PstI CTGCAG 3 cut(s) 229, 1429, 1912
PstNI CAGNNNCTG 1 cut(s) 1903
PsuI RGATCY 3 cut(s) 325, 635, 1039
PvuII CAGCTG 2 cut(s) 274, 1300
RsaI GTAC 7 cut(s) 243, 422, 1062, 1120, 1126, 1256, 1992
RsaNI GTAC 7 cut(s) 242, 421, 1061, 1119, 1125, 1255, 1991
RseI CAYNNNNRTG 4 cut(s) 1371, 1676, 2227, 2229
SacI GAGCTC 2 cut(s) 2094, 2184
SaqAI TTAA 5 cut(s) 552, 615, 752, 938, 2301
Sau3AI GATC 6 cut(s) 121, 325, 503, 635, 763, 1039
Sau96I GGNCC 5 cut(s) 350, 427, 1088, 1142, 1975
ScaI AGTACT 1 cut(s) 1120
SchI GAGTC 3 cut(s) 464, 2054, 2140
SduI GDGCHC 4 cut(s) 406, 626, 2094, 2184
SfcI CTRYAG 3 cut(s) 225, 1425, 1908
SinI GGWCC 4 cut(s) 350, 427, 1088, 1142
SmaI CCCGGG 1 cut(s) 145
SmiI ATTTAAAT 1 cut(s) 939
SmiMI CAYNNNNRTG 4 cut(s) 1371, 1676, 2227, 2229
SnaBI TACGTA 1 cut(s) 1472
SpeI ACTAGT 1 cut(s) 398
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 5 cut(s) 410, 430, 842, 1075, 2280
SspMI CTAG 5 cut(s) 399, 927, 1526, 1839, 2100
SstI GAGCTC 2 cut(s) 2094, 2184
StuI AGGCCT 1 cut(s) 775
StyI CCWWGG 1 cut(s) 343
SwaI ATTTAAAT 1 cut(s) 939
TaaI ACNGT 5 cut(s) 1118, 1259, 1678, 1899, 2253
TaiI ACGT 1 cut(s) 1474
TaqI TCGA 5 cut(s) 580, 1226, 1627, 2144, 2289
TaqII GACCGA 2 cut(s) 116, 1964
TatI WGTACW 2 cut(s) 1060, 1118
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 4 cut(s) 650, 1352, 1810, 1878
Tru1I TTAA 5 cut(s) 552, 615, 752, 938, 2301
Tru9I TTAA 5 cut(s) 552, 615, 752, 938, 2301
TscAI CASTG 6 cut(s) 88, 411, 631, 1237, 1459, 1537
TseFI GTSAC 2 cut(s) 1362, 2253
Tsp45I GTSAC 2 cut(s) 1362, 2253
TspDTI ATGAA 7 cut(s) 85, 993, 1083, 1632, 1653, 1956, 2075
TspGWI ACGGA 5 cut(s) 334, 1209, 1423, 2000, 2287
TspMI CCCGGG 1 cut(s) 143
TspRI CASTG 6 cut(s) 88, 411, 631, 1237, 1459, 1537
Van91I CCANNNNNTGG 1 cut(s) 722
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 4 cut(s) 350, 427, 1088, 1142
XagI CCTNNNNNAGG 2 cut(s) 693, 780
XapI RAATTY 6 cut(s) 358, 934, 1000, 1222, 1586, 1639
XceI RCATGY 4 cut(s) 80, 1745, 2113, 2194
XcmI CCANNNNNNNNNTGG 3 cut(s) 741, 1297, 1889
XmaI CCCGGG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 5 cut(s) 399, 927, 1526, 1839, 2100
ZrmI AGTACT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.