MD10G1165100.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001113
Physical Location & Seq
Reverse (-)
25577515 .. 25583764
6250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1165100.v1.1.491

Sequence Viewer

Length: 2337 bp
ATGGCGCAGAGTAATTGGGAAGCCGATAAGATGCTTGATGTTTATATTCATGATTATTTGTTGAAAAGAAAATTGCTTGCTTCTGCAAATGCGTTTATGACGGAAGGGAAGGTTGCTACGGATCCAGTAGCAATTGATGCACCAGGAGGATTCCTCTTTGAATGGTGGTCTGTCTTTTGGGACATATTCATTGCAAGGACAAATGAGAAGCATTCGGAGGCTGCTGCAGCTTACATTGAGCAAGTTAAGGCAAGGGAGCAACAACAGCTACAAATGCAGCAGCTGCAGCTATTGCAGCAGCGCAGTGCCCAGTTACAGCGCAGGGATCCTAATCATCCTGCCCTTGGTGGTGCTATAAATGCTATAAATTCTGAGGGAGTCATGGGGCAACCGTCAGCCAGTGTGCTAGCCATGAAAATGTATGAAGATCGTATGAAACACCCTCACCCGATGGATTCAGAGGCATCCCCAACGCTCATTGATGCCAGTAGAATGGCCCTGCTCAAATCAGCCGCAAATCCACAAGGCCAGTTGGTACAAGGCAATTCTGGGAACATGCCTGCTGGTTTTCAGCAAATTCAAGCCCGAACTCCTTTAACCACTGACATCAAAACCGAAGTTGGCTTAGGTACACCTCAGAAAAGTTTGCCTATGGATCCTTCATCAATTTATGGACAAGCAATTTTACAGTCAAAATCTGGACTGAGCAGTGCAGGATTGAATCAAGGTATTACAGGTCTTCCCTTGAAGGGTTGGCCTCTAACTGGAATCGACCAATTACGACCAAATTTGGGTGTACAAATGCAGAAGCCAAATCTTCAGACCCAAAATCAGCTCCTTTTGGCATCTCAACAACAACAAGTTTTGGCACAAGCTCAGGCACAAAATAACCTTCGTAATTCCACAAATTACGGAGATATGGATCCTCGCAGATTTTCTGGACTGCCTAGGGGTAACTTGAATGCAAAAGATGGTCAATCTACCAGAAATGATGGATCTATGTCCTCTCCAGTTTTATCAAGTTCACCAAAGATAAAAATGGCTCAGATGCAGCACCCTTCCTCTCAACAACATGAACAATTGCCACAGCAGCAACTGCAACAGAATAACAGAAAAAGGAAACAACACTCTTCCTCTGGACCTGCCAACAGCACTGGCACAGGAAACACTGTTGGCCCTTCACCTAGTTCGCCAACATCAACTCACACGCCTGGTGATGGAATTAACACAGCAAATAGTATGCAGCATGTTAACAGCATGCCAAAAAGCTTGATGATGTATCCAGAAGGAACAGGACTTCCTTCGTCTTCCAATTTACTGGATGACATTGACCGATTTGGAGACGTTGGTTCGCTAGAAGATAATGTGGAATCGTTTTTGTCAAATGATGGGGGAGACGGGAGGGATCTGTATGGGACATTAAAGCAGAGTCTTAAACAGCACCATAAAGATTCCTCTAAAGGTTTTACTTTTGGTGAAGTTGGCTGCATACGGACCAGAAACAGCAAAGTTACTTGCTGTCACTTTTCTTCGGATGGGAAGTTGCTGGCAAGTGCAGGACATGACAAAAAGGTTGTCCTCTGGAACATGGATACTCTGCAGACTGAGAGTACTCCCGAAGAACACACGTTAGTTATCACAGATGTTCGGTTTAGACCAAATTCATCTCAACTGGCAACAGCTTCTTTTGACAAATCTGTACGATTGTGGGATGCAGCCAATCCAAACTATTGTTTACAAGCATATACGGGGCACAACTCACCTATTATGTCCCTTGACTTCCACCCAAAGAAGACTGATTTATTTTGTTTCTGTGATCATGACAATGAAATTCGGTACTGGAATATCAACCCATTCTCTTGTACTCGTAGTTCGAAGGGAGGTACTGCACAAGTGAGGTTTCAGCCAAAAACTGGACAACTACTGGCAGCAGCCTCAGATAAATTAGTTTCCATCTTTGATGTCGAAACTGACAGACAAATGCACTCGTTCCAGGGGCACTCTGACGTTGTAAACTTCATTTGTTGGAATGCAAATGGAGATTATCTGGCTTCTGTCAGTCAGAACTTGGTGAAGATTTGGTCGTTAGTTTCGGGAGATTGTATTCAGGAGCTCGGCTCTAACGGCAACCAGTTTCATTCGTGTGTTTTTCACCCAAGTTATTCAACTCTCTTGGTCATCGGAGGAATCTCGTCTTTGGAGCTGTGGAACATGGCTGAGAACAAGAGCATGACAATTGCAGCACACGAGAATATAATCTCGGCTCTGGCGCAGTCACCTGACACTGGAATGGTTGCGTCGGCAAGCCACGACACCACCGTCAAGTTATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

779

Amino Acids

85.14

Weight (kDa)

6.66

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 490 - 602 4.8e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 490 - 593 3.1e-12 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 493 - 695 1e-15 MABP1/WDR62 second WD40 domain
EIF3I PF24805 496 - 574 1.7e-06 EIF3I
WD40_Prp19 PF24814 499 - 771 1.2e-40 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 499 - 624 1.5e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 501 - 616 1.3e-20 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 501 - 575 1.9e-15 THOC3 beta-propeller domain
WD40 PF00400 501 - 529 6.9e-07 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 501 - 576 1.9e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 501 - 622 7.2e-14 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 501 - 598 3.4e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 501 - 619 5.3e-14 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 502 - 615 2.6e-11 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 535 - 624 1.9e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 538 - 571 3.3e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 543 - 726 1e-18 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 578 - 688 1.8e-08 WDR3 second beta-propeller domain
WDR55 PF24796 591 - 745 4.4e-07 WDR55
Beta-prop_WDR3_1st PF25173 606 - 737 4.7e-16 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 607 - 704 3.2e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 613 - 776 2.6e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 626 - 777 6.6e-26 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 629 - 777 3.4e-22 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 632 - 777 1.4e-13 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 639 - 757 4.1e-06 G protein beta WD-40 repeat protein
WD40 PF00400 660 - 695 4.7e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 672 - 778 2.1e-10 WDR3 second beta-propeller domain
WD40 PF00400 744 - 777 6.8e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2318
Acc36I ACCTGC 1 cut(s) 1150
AccB7I CCANNNNNTGG 1 cut(s) 1913
AciI CCGC 1 cut(s) 513
AcsI RAATTY 5 cut(s) 367, 576, 787, 1660, 1830
AcuI CTGAAG 1 cut(s) 803
AfaI GTAC 8 cut(s) 537, 631, 798, 1612, 1701, 1838, 1864, 1885
AfiI CCNNNNNNNGG 7 cut(s) 344, 749, 764, 791, 1217, 1913, 2285
AflIII ACRYGT 1 cut(s) 1626
AgsI TTSAA 7 cut(s) 64, 161, 581, 721, 748, 961, 2166
AjnI CCWGG 3 cut(s) 142, 1210, 1992
AloI GAACNNNNNNTCC 2 cut(s) 1282, 1314
Alw21I GWGCWC 1 cut(s) 2115
Alw26I GTCTC 2 cut(s) 1335, 1389
AlwNI CAGNNNCTG 2 cut(s) 283, 1096
AoxI GGCC 4 cut(s) 495, 526, 755, 1174
ApoI RAATTY 5 cut(s) 367, 576, 787, 1660, 1830
ArsI GACNNNNNNTTYG 2 cut(s) 1560, 1592
AspA2I CCTAGG 1 cut(s) 947
AspLEI GCGC 4 cut(s) 7, 303, 321, 2272
AspS9I GGNCC 4 cut(s) 496, 1139, 1175, 1494
AsuHPI GGTGA 9 cut(s) 437, 1017, 1173, 1226, 1487, 1752, 2083, 2144, 2268
AsuII TTCGAA 1 cut(s) 1874
AsuNHI GCTAGC 1 cut(s) 406
AvaII GGWCC 2 cut(s) 1139, 1494
AvrII CCTAGG 1 cut(s) 947
BaeGI GKGCMC 3 cut(s) 310, 1755, 2001
BamHI GGATCC 4 cut(s) 121, 325, 655, 922
BanII GRGCYC 1 cut(s) 2115
BauI CACGAG 1 cut(s) 2246
BbsI GAAGAC 3 cut(s) 731, 1299, 1799
Bbv12I GWGCWC 1 cut(s) 2115
BccI CCATC 7 cut(s) 445, 965, 986, 1211, 1382, 1529, 1961
BceAI ACGGC 1 cut(s) 2140
BciT130I CCWGG 3 cut(s) 144, 1212, 1994
BciVI GTATCC 2 cut(s) 1290, 1585
BclI TGATCA 1 cut(s) 1816
BcoDI GTCTC 2 cut(s) 1335, 1389
BfaI CTAG 4 cut(s) 407, 948, 1185, 1355
BfmI CTRYAG 3 cut(s) 225, 284, 1598
BfuAI ACCTGC 1 cut(s) 1150
BfuI GTATCC 2 cut(s) 1290, 1585
BlnI CCTAGG 1 cut(s) 947
BmcAI AGTACT 1 cut(s) 1612
Bme1390I CCNGG 3 cut(s) 144, 1212, 1994
Bme18I GGWCC 2 cut(s) 1139, 1494
BmgT120I GGNCC 4 cut(s) 496, 1139, 1175, 1494
BmiI GGNNCC 4 cut(s) 123, 327, 657, 924
BmrFI CCNGG 3 cut(s) 144, 1212, 1994
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 7 cut(s) 21, 127, 472, 473, 854, 1038, 1702
BmtI GCTAGC 1 cut(s) 410
BmuI ACTGGG 1 cut(s) 304
BpiI GAAGAC 3 cut(s) 731, 1299, 1799
BplI GAGNNNNNCTC 4 cut(s) 138, 170, 2102, 2134
BpmI CTGGAG 1 cut(s) 993
Bpu10I CCTNAGC 2 cut(s) 625, 876
Bpu14I TTCGAA 1 cut(s) 1874
BsaBI GATNNNNATC 2 cut(s) 330, 921
BsaJI CCNNGG 3 cut(s) 343, 947, 1993
Bsc4I CCNNNNNNNGG 7 cut(s) 344, 749, 764, 791, 1217, 1913, 2285
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 2 cut(s) 330, 921
BseBI CCWGG 3 cut(s) 144, 1212, 1994
BseDI CCNNGG 3 cut(s) 343, 947, 1993
BseGI GGATG 5 cut(s) 334, 464, 1327, 1540, 1717
BseJI GATNNNNATC 2 cut(s) 330, 921
BseLI CCNNNNNNNGG 7 cut(s) 344, 749, 764, 791, 1217, 1913, 2285
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 8 cut(s) 363, 650, 695, 890, 1058, 1596, 1950, 2208
BseSI GKGCMC 3 cut(s) 310, 1755, 2001
BsgI GTGCAG 3 cut(s) 732, 1575, 1872
BshFI GGCC 4 cut(s) 497, 528, 757, 1176
BsiHKAI GWGCWC 1 cut(s) 2115
BslFI GGGAC 3 cut(s) 194, 1429, 1756
BslI CCNNNNNNNGG 7 cut(s) 344, 749, 764, 791, 1217, 1913, 2285
BsmAI GTCTC 2 cut(s) 1335, 1389
BsmBI CGTCTC 2 cut(s) 1335, 1389
BsmFI GGGAC 3 cut(s) 194, 1429, 1756
BsmI GAATGC 3 cut(s) 211, 967, 2035
BsnI GGCC 4 cut(s) 497, 528, 757, 1176
Bsp119I TTCGAA 1 cut(s) 1874
Bsp1286I GDGCHC 4 cut(s) 310, 1755, 2001, 2115
Bsp1407I TGTACA 1 cut(s) 796
Bsp143I GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
BspACI CCGC 1 cut(s) 513
BspANI GGCC 4 cut(s) 497, 528, 757, 1176
BspCNI CTCAG 8 cut(s) 364, 649, 696, 889, 1057, 1597, 1949, 2209
BspHI TCATGA 2 cut(s) 49, 1819
BspLI GGNNCC 4 cut(s) 123, 327, 657, 924
BspMAI CTGCAG 3 cut(s) 229, 288, 1602
BspMI ACCTGC 1 cut(s) 1150
BspOI GCTAGC 1 cut(s) 410
BspT104I TTCGAA 1 cut(s) 1874
BsrDI GCAATG 1 cut(s) 189
BsrGI TGTACA 1 cut(s) 796
BssECI CCNNGG 3 cut(s) 343, 947, 1993
BssMI GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
BssSI CACGAG 1 cut(s) 2246
BssT1I CCWWGG 2 cut(s) 343, 947
Bst2BI CACGAG 1 cut(s) 2246
Bst2UI CCWGG 3 cut(s) 144, 1212, 1994
Bst4CI ACNGT 4 cut(s) 393, 690, 1171, 2320
Bst6I CTCTTC 1 cut(s) 1135
BstAPI GCANNNNNTGC 4 cut(s) 137, 283, 292, 1096
BstAUI TGTACA 1 cut(s) 796
BstBI TTCGAA 1 cut(s) 1874
BstC8I GCNNGC 6 cut(s) 78, 408, 561, 1259, 1548, 2305
BstDEI CTNAG 9 cut(s) 372, 625, 636, 704, 876, 1044, 1605, 1936, 2217
BstF5I GGATG 5 cut(s) 334, 464, 1327, 1540, 1717
BstHHI GCGC 4 cut(s) 7, 303, 321, 2272
BstKTI GATC 8 cut(s) 124, 328, 430, 658, 925, 998, 1408, 1819
BstMAI GTCTC 2 cut(s) 1335, 1389
BstMBI GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
BstNI CCWGG 3 cut(s) 144, 1212, 1994
BstNSI RCATGY 3 cut(s) 559, 1250, 1261
BstSCI CCNGG 3 cut(s) 142, 1210, 1992
BstSFI CTRYAG 3 cut(s) 225, 284, 1598
BstSLI GKGCMC 3 cut(s) 310, 1755, 2001
BstV2I GAAGAC 3 cut(s) 731, 1299, 1799
BstX2I RGATCY 6 cut(s) 121, 325, 655, 922, 995, 1405
BstXI CCANNNNNNTGG 2 cut(s) 493, 1318
BstYI RGATCY 6 cut(s) 121, 325, 655, 922, 995, 1405
BsuI GTATCC 2 cut(s) 1290, 1585
BsuRI GGCC 4 cut(s) 497, 528, 757, 1176
BtsCI GGATG 5 cut(s) 334, 464, 1327, 1540, 1717
BtsI GCAGTG 2 cut(s) 310, 715
BtsIMutI CAGTG 7 cut(s) 310, 406, 600, 715, 1152, 1167, 2283
BveI ACCTGC 1 cut(s) 1150
Cac8I GCNNGC 6 cut(s) 78, 408, 561, 1259, 1548, 2305
CaiI CAGNNNCTG 2 cut(s) 283, 1096
CciI TCATGA 2 cut(s) 49, 1819
CfoI GCGC 4 cut(s) 7, 303, 321, 2272
Cfr13I GGNCC 4 cut(s) 496, 1139, 1175, 1494
CseI GACGC 1 cut(s) 2286
Csp6I GTAC 8 cut(s) 536, 630, 797, 1611, 1700, 1837, 1863, 1884
CviQI GTAC 8 cut(s) 536, 630, 797, 1611, 1700, 1837, 1863, 1884
DdeI CTNAG 9 cut(s) 372, 625, 636, 704, 876, 1044, 1605, 1936, 2217
DpnI GATC 8 cut(s) 123, 327, 429, 657, 924, 997, 1407, 1818
DpnII GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
DrdI GACNNNNNNGTC 1 cut(s) 2318
DseDI GACNNNNNNGTC 1 cut(s) 2318
Eam1104I CTCTTC 1 cut(s) 1135
EarI CTCTTC 1 cut(s) 1135
Ecl136II GAGCTC 1 cut(s) 2113
Eco130I CCWWGG 2 cut(s) 343, 947
Eco24I GRGCYC 1 cut(s) 2115
Eco47I GGWCC 2 cut(s) 1139, 1494
Eco53kI GAGCTC 1 cut(s) 2113
Eco57I CTGAAG 1 cut(s) 803
EcoICRI GAGCTC 1 cut(s) 2113
EcoRII CCWGG 3 cut(s) 142, 1210, 1992
EcoT14I CCWWGG 2 cut(s) 343, 947
EcoT38I GRGCYC 1 cut(s) 2115
ErhI CCWWGG 2 cut(s) 343, 947
Esp3I CGTCTC 2 cut(s) 1335, 1389
FaqI GGGAC 3 cut(s) 194, 1429, 1756
FbaI TGATCA 1 cut(s) 1816
FokI GGATG 5 cut(s) 321, 451, 1334, 1547, 1724
FriOI GRGCYC 1 cut(s) 2115
FspBI CTAG 4 cut(s) 407, 948, 1185, 1355
GlaI GCGC 4 cut(s) 6, 302, 320, 2271
GsuI CTGGAG 1 cut(s) 993
HaeIII GGCC 4 cut(s) 497, 528, 757, 1176
HgaI GACGC 1 cut(s) 2286
HhaI GCGC 4 cut(s) 7, 303, 321, 2272
Hin6I GCGC 4 cut(s) 5, 301, 319, 2270
HinP1I GCGC 4 cut(s) 5, 301, 319, 2270
HincII GTYRAC 1 cut(s) 1252
HindII GTYRAC 1 cut(s) 1252
HindIII AAGCTT 1 cut(s) 1267
HinfI GANTC 9 cut(s) 150, 378, 455, 721, 768, 1370, 1429, 1451, 2187
HpaI GTTAAC 1 cut(s) 1252
HphI GGTGA 9 cut(s) 437, 1017, 1173, 1226, 1487, 1752, 2083, 2144, 2268
Hpy166II GTNNAC 6 cut(s) 632, 797, 1025, 1252, 1736, 2014
Hpy8I GTNNAC 6 cut(s) 632, 797, 1025, 1252, 1736, 2014
Hpy99I CGWCG 1 cut(s) 2302
HpyCH4III ACNGT 4 cut(s) 393, 690, 1171, 2320
HpyCH4IV ACGT 3 cut(s) 1344, 1628, 2007
HpyF3I CTNAG 9 cut(s) 372, 625, 636, 704, 876, 1044, 1605, 1936, 2217
HpySE526I ACGT 3 cut(s) 1344, 1628, 2007
HspAI GCGC 4 cut(s) 5, 301, 319, 2270
Ksp22I TGATCA 1 cut(s) 1816
KspAI GTTAAC 1 cut(s) 1252
Kzo9I GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
LmnI GCTCC 4 cut(s) 256, 840, 2110, 2200
LweI GCATC 7 cut(s) 21, 127, 472, 473, 854, 1038, 1702
MaeI CTAG 4 cut(s) 407, 948, 1185, 1355
MaeII ACGT 3 cut(s) 1344, 1628, 2007
MaeIII GTNAC 5 cut(s) 312, 953, 1510, 1520, 2274
MalI GATC 8 cut(s) 123, 327, 429, 657, 924, 997, 1407, 1818
MboI GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
MfeI CAATTG 3 cut(s) 132, 1079, 2235
MflI RGATCY 6 cut(s) 121, 325, 655, 922, 995, 1405
MhlI GDGCHC 4 cut(s) 310, 1755, 2001, 2115
MlyI GAGTC 2 cut(s) 387, 1438
MmeI TCCRAC 1 cut(s) 2006
MseI TTAA 6 cut(s) 246, 596, 1224, 1251, 1421, 1434
MslI CAYNNNNRTG 4 cut(s) 416, 1824, 2142, 2288
MspA1I CMGCKG 1 cut(s) 283
MspR9I CCNGG 3 cut(s) 144, 1212, 1994
MunI CAATTG 3 cut(s) 132, 1079, 2235
Mva1269I GAATGC 3 cut(s) 211, 967, 2035
MvaI CCWGG 3 cut(s) 144, 1212, 1994
NdeII GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
NheI GCTAGC 1 cut(s) 406
NlaIV GGNNCC 4 cut(s) 123, 327, 657, 924
NmeAIII GCCGAG 2 cut(s) 2094, 2240
NmuCI GTSAC 2 cut(s) 1520, 2274
NspI RCATGY 3 cut(s) 559, 1250, 1261
NspV TTCGAA 1 cut(s) 1874
PaeI GCATGC 1 cut(s) 1261
PagI TCATGA 2 cut(s) 49, 1819
PcsI WCGNNNNNNNCGW 1 cut(s) 2316
PctI GAATGC 3 cut(s) 211, 967, 2035
PfeI GAWTC 7 cut(s) 150, 455, 721, 768, 1370, 1451, 2187
PflMI CCANNNNNTGG 1 cut(s) 1913
PleI GAGTC 2 cut(s) 386, 1437
PpsI GAGTC 2 cut(s) 386, 1437
Psp124BI GAGCTC 1 cut(s) 2115
Psp6I CCWGG 3 cut(s) 142, 1210, 1992
PspGI CCWGG 3 cut(s) 142, 1210, 1992
PspN4I GGNNCC 4 cut(s) 123, 327, 657, 924
PspPI GGNCC 4 cut(s) 496, 1139, 1175, 1494
PsrI GAACNNNNNNTAC 2 cut(s) 1855, 1887
PstI CTGCAG 3 cut(s) 229, 288, 1602
PstNI CAGNNNCTG 2 cut(s) 283, 1096
PsuI RGATCY 6 cut(s) 121, 325, 655, 922, 995, 1405
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 8 cut(s) 537, 631, 798, 1612, 1701, 1838, 1864, 1885
RsaNI GTAC 8 cut(s) 536, 630, 797, 1611, 1700, 1837, 1863, 1884
RseI CAYNNNNRTG 4 cut(s) 416, 1824, 2142, 2288
SacI GAGCTC 1 cut(s) 2115
SaqAI TTAA 6 cut(s) 246, 596, 1224, 1251, 1421, 1434
Sau3AI GATC 8 cut(s) 121, 325, 427, 655, 922, 995, 1405, 1816
Sau96I GGNCC 4 cut(s) 496, 1139, 1175, 1494
ScaI AGTACT 1 cut(s) 1612
SchI GAGTC 2 cut(s) 387, 1438
ScrFI CCNGG 3 cut(s) 144, 1212, 1994
SduI GDGCHC 4 cut(s) 310, 1755, 2001, 2115
SfaNI GCATC 7 cut(s) 21, 127, 472, 473, 854, 1038, 1702
SfcI CTRYAG 3 cut(s) 225, 284, 1598
SfuI TTCGAA 1 cut(s) 1874
SinI GGWCC 2 cut(s) 1139, 1494
SmiMI CAYNNNNRTG 4 cut(s) 416, 1824, 2142, 2288
SphI GCATGC 1 cut(s) 1261
SsiI CCGC 1 cut(s) 513
SspMI CTAG 4 cut(s) 407, 948, 1185, 1355
SstI GAGCTC 1 cut(s) 2115
StyD4I CCNGG 3 cut(s) 142, 1210, 1992
StyI CCWWGG 2 cut(s) 343, 947
TaaI ACNGT 4 cut(s) 393, 690, 1171, 2320
TaiI ACGT 3 cut(s) 1347, 1631, 2010
TaqI TCGA 3 cut(s) 771, 1874, 1965
TaqII GACCGA 1 cut(s) 1347
TatI WGTACW 3 cut(s) 796, 1610, 1862
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 7 cut(s) 150, 455, 721, 768, 1370, 1451, 2187
Tru1I TTAA 6 cut(s) 246, 596, 1224, 1251, 1421, 1434
Tru9I TTAA 6 cut(s) 246, 596, 1224, 1251, 1421, 1434
TscAI CASTG 7 cut(s) 310, 406, 607, 715, 1159, 1174, 2290
TseFI GTSAC 2 cut(s) 1520, 2274
Tsp45I GTSAC 2 cut(s) 1520, 2274
TspGWI ACGGA 4 cut(s) 116, 134, 927, 1507
TspRI CASTG 7 cut(s) 310, 406, 607, 715, 1159, 1174, 2290
Van91I CCANNNNNTGG 1 cut(s) 1913
VpaK11BI GGWCC 2 cut(s) 1139, 1494
XapI RAATTY 5 cut(s) 367, 576, 787, 1660, 1830
XceI RCATGY 3 cut(s) 559, 1250, 1261
XmaJI CCTAGG 1 cut(s) 947
XspI CTAG 4 cut(s) 407, 948, 1185, 1355
ZrmI AGTACT 1 cut(s) 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.