Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0006285

Physical Location & Seq

Genomic Coordinates Reverse (-)

647412 .. 650983

Gene Structure 3572 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g00721.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1722 bp

ATGTCCCTTGAACCGGACCCCATTTTCATAAAAGGTTCTCCTCCTCCTTCACTGAAGAGCAGCCATTTCACTTTGCTCTCACCCAAAATGCTCACAACAAGGCTCCAAAATCTCAAGCTCCATTCGTCTCTAAAACCCCACATCAAATCCCTGCGTCACTTCTTAAACCCTCTATGCACCACACCCGAGTCACCGGAGCTTCCAAGCTGGGTCAAGTTCTTCGACTCCAACCACCCAAAACCCGCCGCGTCTTCTTCTTCCGACGACGACTTCGTCATCCCTTCTCTGGCCAACTGGGTCGAGACCCACAACCTAAACCACCCCACCAAACCCCCCAAGCCCTCAGCTTCCGACGCCGACGAGACCCTCGAAAGCATCAGCAAAATCTTGAAGCTCCGGTACCCTTCGCCGGAAAACGCAGCTGAAGCGTTGGAAAGGTGTGATTTTGAGGTGTCAGATGGTCTGGTGCAACGGGTTTTGATGAGGTTTAGCAATGATTGGGTCCCTGCTTTGGGTTTTTTCACTTGGGCAAAACGTCGAACAGGTTATGTGCATTGTCATGAGGCCTATAACTCCATTGTTGATGTGTTGGGAAAGTCGACAAATTTCGAACTTATGTGGGATGTTGTTGAGGAAATGGATCGGATTGGAAAAGGATATGTGTCATTGGAGACATTGACAAAGGTGTTTAGGAGGCTTGCAAAGGGTGGTAAGCACAAAGAAGCAATCGAGGCGTTTCGGGGCATGGAGAGGTTCGGATTGAGCAAAGATGTTGTGGCCTTGAACACTCTTTTGAGTGCATTGGTGAAGGAGAGGAGCGTTGAGCTTGCGCAAGAAGCTTTCTTGGAGTTCAAGGAATCTGTACCTGTGAATGATCACAGTTTTAATATTTTGATACATGGGTGGTGTAAGAATAGGAAGATGGATATTGCTAGGAGCACAATGGAGGAGATGGAGAGACATGGGTTTCGCCCTGATGTTTTCTCTTATACTTGTTTGGTTGAGGCTTATTGTAGTGACAAGGATTTTCGGAAAGTTGATGAAGTTGTTAGTGAAATGAAGGGAAAGGGTTGCGCGCCGAATGCTGTGACTTACACTGTCATACTGCATGCTTTGGGGAAGGCAAGGGAGATAACCAAGGCTTTGGAGGTTTATGAGGAAATGAAAGGGAATGGTGTTGTTCCTGATGTATCCTTTTACAGCTCTTTGCTATACATTCTTGGTAAAGCTGGCAGGCTTAAGGATGTTCAAGAAGTCGTTGAGGACATGACAAAGCAGGGTGTAAAACCGAACGTTGTGACCTATAACACCTTGATTTCTTGTGCTTGCGAGCATTCGCAGGAAGAGGGTGCTCTCAAGTTGCTGAAAAAAATGGAGGATTCGTGCAAGCCTGATGTTGAGACTTATGCCCCCTTGTTGAAGATGTGCTGCAGAAAGAAGAGGATGAAGGTGCTCTACTTTCTGTTAGACCACATGTTTAAGAATGACATAAGTATTGATGCTGGGACCTATGCGCTTTTGGTACGCGGGATGTGCAAGAATGGTAAACTTGAAAACGCTTCCGCCTTCTTTGAGGAGATGGTATTGAAGGGTTTTGTTCCCAAGGACAGCACATACAAGATGATGATAGAGGAATTGCAGAGGAAGAGTATGGAGGAAAAGAAGAAAGATATTGAGAAGTTGATGTTGCAGGCAAAAGAGAAAGGCAACTTAACTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

574

Amino Acids

64.96

Weight (kDa)

7.85

Isoelectric Point (pI)

40.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	256 - 301	9.6e-06	PPR repeat family
PPR_long	PF17177	285 - 419	1.8e-14	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	292 - 338	1.7e-13	PPR repeat family
PPR_3	PF13812	315 - 374	6.7e-15	Pentatricopeptide repeat domain
PPR_2	PF13041	325 - 374	1.9e-14	PPR repeat family
PPR_1	PF12854	356 - 389	5.5e-07	PPR repeat
PPR_2	PF13041	360 - 404	3.8e-10	PPR repeat family
PPR_3	PF13812	363 - 409	3.6e-06	Pentatricopeptide repeat domain
PPR	PF01535	363 - 393	2.4e-06	PPR repeat
TPR_24	PF23276	364 - 457	2.2e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	395 - 443	1.8e-11	PPR repeat family
PPR_long	PF17177	400 - 541	2e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	419 - 476	2.3e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	427 - 459	7.2e-09	PPR repeat
PPR_2	PF13041	430 - 478	3.9e-12	PPR repeat family
PPR_2	PF13041	464 - 513	2.9e-07	PPR repeat family
PPR_1	PF12854	497 - 528	1.8e-07	PPR repeat
PPR_2	PF13041	501 - 546	3.3e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22670
fragaria_vesca	FvH4_4g00721
malus_domestica	MD13G1197300.v1.1
prunus_persica	Prupe.1G018400_v2.0.a1
pyrus_communis	pycom13g17020
rosa_chinensis	RchiOBHm_Chr1g0322101 RchiOBHm_Chr4g0386451
rosa_laevigata	RLG00000010131 RLG00000010157 RLG00000010161
rosa_multiflora	Rmu_co8274927.1_g000001 Rmu_sc0001942.1_g000008 Rmu_sc0001942.1_g000027 Rmu_sc0001942.1_g000051 Rmu_sc0002785.1_g000008 Rmu_sc0003786.1_g000023 Rmu_sc0006648.1_g000016 Rmu_sc0036937.1_g000001 Rmu_ssc0000272.1_g000008 Rmu_ssc0000272.1_g000009
rosa_roxburghii	Rroxscaffold_5G00333960 Rroxscaffold_6G00392780
rosa_samantha	Rh1AG049600 Rh4BG009300 Rh4CG014300 Rh4DG010100
rosa_wichuraiana	Rw4G000030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	272
Acc16I	TGCGCA	1 cut(s)	831
Acc65I	GGTACC	1 cut(s)	399
AccB1I	GGYRCC	1 cut(s)	399
AccI	GTMKAC	1 cut(s)	599
AccII	CGCG	3 cut(s)	248, 1076, 1527
AciI	CCGC	4 cut(s)	243, 246, 1527, 1563
AclI	AACGTT	1 cut(s)	1293
AclWI	GGATC	1 cut(s)	648
AcoI	YGGCCR	1 cut(s)	288
AcsI	RAATTY	1 cut(s)	604
AcuI	CTGAAG	2 cut(s)	74, 444
AcyI	GRCGYC	1 cut(s)	354
AfaI	GTAC	3 cut(s)	401, 864, 1524
AfiI	CCNNNNNNNGG	5 cut(s)	13, 286, 409, 511, 512
AflII	CTTAAG	1 cut(s)	1238
AflIII	ACRYGT	1 cut(s)	1473
AgsI	TTSAA	8 cut(s)	11, 391, 784, 853, 1250, 1420, 1553, 1588
Alw21I	GWGCWC	3 cut(s)	941, 1354, 1455
Alw26I	GTCTC	6 cut(s)	132, 296, 356, 665, 952, 1394
AlwI	GGATC	1 cut(s)	648
Ama87I	CYCGRG	1 cut(s)	185
AoxI	GGCC	3 cut(s)	288, 564, 777
ApeKI	GCWGC	3 cut(s)	60, 419, 1428
ApoI	RAATTY	1 cut(s)	604
ArsI	GACNNNNNNTTYG	4 cut(s)	254, 286, 951, 983
Asp718I	GGTACC	1 cut(s)	399
AspLEI	GCGC	4 cut(s)	832, 1076, 1078, 1516
AspS9I	GGNCC	3 cut(s)	16, 502, 1506
AsuHPI	GGTGA	3 cut(s)	72, 183, 817
AsuII	TTCGAA	1 cut(s)	609
AvaI	CYCGRG	1 cut(s)	185
AvaII	GGWCC	3 cut(s)	16, 502, 1506
BaeI	ACNNNNGTAYC	2 cut(s)	887, 920
BalI	TGGCCA	1 cut(s)	290
BanI	GGYRCC	1 cut(s)	399
BarI	GAAGNNNNNNTAC	4 cut(s)	383, 415, 1439, 1471
BbsI	GAAGAC	1 cut(s)	243
Bbv12I	GWGCWC	3 cut(s)	941, 1354, 1455
BbvCI	CCTCAGC	1 cut(s)	343
BbvI	GCAGC	3 cut(s)	72, 431, 1415
BccI	CCATC	4 cut(s)	452, 916, 946, 1573
BciVI	GTATCC	1 cut(s)	1201
BclI	TGATCA	1 cut(s)	874
BcoDI	GTCTC	6 cut(s)	132, 296, 356, 665, 952, 1394
BfaI	CTAG	1 cut(s)	933
BfmI	CTRYAG	1 cut(s)	1429
BfrI	CTTAAG	1 cut(s)	1238
BfuI	GTATCC	1 cut(s)	1201
BisI	GCNGC	4 cut(s)	61, 246, 420, 1429
BlsI	GCNGC	4 cut(s)	62, 247, 421, 1430
Bme18I	GGWCC	3 cut(s)	16, 502, 1506
BmeT110I	CYCGRG	1 cut(s)	185
BmgT120I	GGNCC	3 cut(s)	16, 502, 1506
BmiI	GGNNCC	6 cut(s)	18, 104, 401, 503, 504, 1507
BmrI	ACTGGG	1 cut(s)	304
BmsI	GCATC	2 cut(s)	384, 1489
BmuI	ACTGGG	1 cut(s)	304
BpiI	GAAGAC	1 cut(s)	243
Bpu10I	CCTNAGC	1 cut(s)	343
Bpu14I	TTCGAA	1 cut(s)	609
BpuEI	CTTGAG	2 cut(s)	98, 1340
BsaHI	GRCGYC	1 cut(s)	354
BsaI	GGTCTC	2 cut(s)	296, 356
BsaJI	CCNNGG	2 cut(s)	1137, 1602
BsaWI	WCCGGW	3 cut(s)	13, 193, 396
BsaXI	ACNNNNNCTCC	2 cut(s)	1367, 1397
Bsc4I	CCNNNNNNNGG	5 cut(s)	13, 286, 409, 511, 512
Bse1I	ACTGG	1 cut(s)	299
Bse3DI	GCAATG	1 cut(s)	499
BseDI	CCNNGG	2 cut(s)	1137, 1602
BseGI	GGATG	5 cut(s)	276, 628, 1249, 1449, 1536
BseLI	CCNNNNNNNGG	5 cut(s)	13, 286, 409, 511, 512
BseMI	GCAATG	1 cut(s)	499
BseMII	CTCAG	1 cut(s)	357
BseNI	ACTGG	1 cut(s)	299
BsePI	GCGCGC	1 cut(s)	1074
BseRI	GAGGAG	5 cut(s)	30, 33, 829, 962, 1589
BseXI	GCAGC	3 cut(s)	72, 431, 1415
BseYI	CCCAGC	2 cut(s)	207, 1502
Bsh1236I	CGCG	3 cut(s)	248, 1076, 1527
BshFI	GGCC	3 cut(s)	290, 566, 779
BshNI	GGYRCC	1 cut(s)	399
BsiHKAI	GWGCWC	3 cut(s)	941, 1354, 1455
BsiHKCI	CYCGRG	1 cut(s)	185
BsiSI	CCGG	4 cut(s)	14, 194, 397, 410
BslFI	GGGAC	2 cut(s)	488, 1519
BslI	CCNNNNNNNGG	5 cut(s)	13, 286, 409, 511, 512
BsmAI	GTCTC	6 cut(s)	132, 296, 356, 665, 952, 1394
BsmBI	CGTCTC	1 cut(s)	132
BsmFI	GGGAC	2 cut(s)	488, 1519
BsmI	GAATGC	2 cut(s)	1087, 1333
BsnI	GGCC	3 cut(s)	290, 566, 779
Bso31I	GGTCTC	2 cut(s)	296, 356
BsoBI	CYCGRG	1 cut(s)	185
Bsp119I	TTCGAA	1 cut(s)	609
Bsp1286I	GDGCHC	3 cut(s)	941, 1354, 1455
Bsp143I	GATC	2 cut(s)	640, 874
BspACI	CCGC	4 cut(s)	243, 246, 1527, 1563
BspANI	GGCC	3 cut(s)	290, 566, 779
BspCNI	CTCAG	1 cut(s)	356
BspFNI	CGCG	3 cut(s)	248, 1076, 1527
BspHI	TCATGA	1 cut(s)	559
BspLI	GGNNCC	6 cut(s)	18, 104, 401, 503, 504, 1507
BspMAI	CTGCAG	1 cut(s)	1433
BspPI	GGATC	1 cut(s)	648
BspQI	GCTCTTC	1 cut(s)	50
BspT104I	TTCGAA	1 cut(s)	609
BspT107I	GGYRCC	1 cut(s)	399
BspTI	CTTAAG	1 cut(s)	1238
BspTNI	GGTCTC	2 cut(s)	296, 356
BsrDI	GCAATG	1 cut(s)	499
BsrI	ACTGG	1 cut(s)	299
BssECI	CCNNGG	2 cut(s)	1137, 1602
BssHII	GCGCGC	1 cut(s)	1074
BssMI	GATC	2 cut(s)	640, 874
BssNI	GRCGYC	1 cut(s)	354
BssT1I	CCWWGG	2 cut(s)	1137, 1602
Bst4CI	ACNGT	2 cut(s)	881, 1099
Bst6I	CTCTTC	4 cut(s)	50, 1338, 1433, 1640
BstACI	GRCGYC	1 cut(s)	354
BstAFI	CTTAAG	1 cut(s)	1238
BstBI	TTCGAA	1 cut(s)	609
BstDEI	CTNAG	1 cut(s)	343
BstF5I	GGATG	5 cut(s)	276, 628, 1249, 1449, 1536
BstFNI	CGCG	3 cut(s)	248, 1076, 1527
BstHHI	GCGC	4 cut(s)	832, 1076, 1078, 1516
BstKTI	GATC	2 cut(s)	643, 877
BstMAI	GTCTC	6 cut(s)	132, 296, 356, 665, 952, 1394
BstMBI	GATC	2 cut(s)	640, 874
BstMWI	GCNNNNNNNGC	6 cut(s)	353, 425, 731, 836, 1082, 1533
BstNSI	RCATGY	2 cut(s)	1112, 1477
BstSFI	CTRYAG	1 cut(s)	1429
BstUI	CGCG	3 cut(s)	248, 1076, 1527
BstV1I	GCAGC	3 cut(s)	72, 431, 1415
BstV2I	GAAGAC	1 cut(s)	243
BstXI	CCANNNNNNTGG	1 cut(s)	1144
BsuI	GTATCC	1 cut(s)	1201
BsuRI	GGCC	3 cut(s)	290, 566, 779
BtsCI	GGATG	5 cut(s)	276, 628, 1249, 1449, 1536
BtsIMutI	CAGTG	2 cut(s)	50, 1095
CciI	TCATGA	1 cut(s)	559
CfoI	GCGC	4 cut(s)	832, 1076, 1078, 1516
Cfr13I	GGNCC	3 cut(s)	16, 502, 1506
CseI	GACGC	3 cut(s)	143, 237, 362
Csp6I	GTAC	3 cut(s)	400, 863, 1523
CviAII	CATG	7 cut(s)	560, 745, 899, 962, 1109, 1267, 1474
CviQI	GTAC	3 cut(s)	400, 863, 1523
DdeI	CTNAG	1 cut(s)	343
DpnI	GATC	2 cut(s)	642, 876
DpnII	GATC	2 cut(s)	640, 874
DrdI	GACNNNNNNGTC	1 cut(s)	272
DseDI	GACNNNNNNGTC	1 cut(s)	272
EaeI	YGGCCR	1 cut(s)	288
Eam1104I	CTCTTC	4 cut(s)	50, 1338, 1433, 1640
EarI	CTCTTC	4 cut(s)	50, 1338, 1433, 1640
EciI	GGCGGA	1 cut(s)	1552
Eco130I	CCWWGG	2 cut(s)	1137, 1602
Eco147I	AGGCCT	1 cut(s)	566
Eco31I	GGTCTC	2 cut(s)	296, 356
Eco47I	GGWCC	3 cut(s)	16, 502, 1506
Eco57I	CTGAAG	2 cut(s)	74, 444
Eco88I	CYCGRG	1 cut(s)	185
EcoO109I	RGGNCCY	2 cut(s)	502, 1506
EcoT14I	CCWWGG	2 cut(s)	1137, 1602
ErhI	CCWWGG	2 cut(s)	1137, 1602
Esp3I	CGTCTC	1 cut(s)	132
FaeI	CATG	7 cut(s)	563, 748, 902, 965, 1112, 1270, 1477
FaqI	GGGAC	2 cut(s)	488, 1519
FatI	CATG	7 cut(s)	559, 744, 898, 961, 1108, 1266, 1473
FauI	CCCGC	2 cut(s)	250, 1520
FbaI	TGATCA	1 cut(s)	874
FblI	GTMKAC	1 cut(s)	599
Fnu4HI	GCNGC	4 cut(s)	61, 246, 420, 1429
FokI	GGATG	5 cut(s)	263, 635, 1256, 1456, 1543
Fsp4HI	GCNGC	4 cut(s)	61, 246, 420, 1429
FspBI	CTAG	1 cut(s)	933
FspI	TGCGCA	1 cut(s)	831
GlaI	GCGC	4 cut(s)	831, 1075, 1077, 1515
GluI	GCNGC	4 cut(s)	61, 246, 420, 1429
GsaI	CCCAGC	2 cut(s)	211, 1506
HaeIII	GGCC	3 cut(s)	290, 566, 779
HapII	CCGG	4 cut(s)	14, 194, 397, 410
HgaI	GACGC	3 cut(s)	143, 237, 362
HhaI	GCGC	4 cut(s)	832, 1076, 1078, 1516
Hin1I	GRCGYC	1 cut(s)	354
Hin1II	CATG	7 cut(s)	563, 748, 902, 965, 1112, 1270, 1477
Hin6I	GCGC	4 cut(s)	830, 1074, 1076, 1514
HinP1I	GCGC	4 cut(s)	830, 1074, 1076, 1514
HincII	GTYRAC	1 cut(s)	600
HindII	GTYRAC	1 cut(s)	600
HindIII	AAGCTT	1 cut(s)	837
HinfI	GANTC	4 cut(s)	188, 224, 857, 1379
HpaII	CCGG	4 cut(s)	14, 194, 397, 410
HphI	GGTGA	3 cut(s)	72, 183, 817
Hpy166II	GTNNAC	2 cut(s)	600, 1547
Hpy188I	TCNGA	6 cut(s)	262, 352, 457, 645, 758, 1032
Hpy188III	TCNNGA	5 cut(s)	301, 388, 560, 1184, 1250
Hpy8I	GTNNAC	2 cut(s)	600, 1547
Hpy99I	CGWCG	5 cut(s)	266, 269, 356, 362, 540
HpyAV	CCTTC	9 cut(s)	57, 291, 414, 802, 1054, 1114, 1441, 1576, 1582
HpyCH4III	ACNGT	2 cut(s)	881, 1099
HpyCH4IV	ACGT	2 cut(s)	535, 1293
HpyF10VI	GCNNNNNNNGC	6 cut(s)	353, 425, 731, 836, 1082, 1533
HpyF3I	CTNAG	1 cut(s)	343
HpySE526I	ACGT	2 cut(s)	535, 1293
Hsp92I	GRCGYC	1 cut(s)	354
Hsp92II	CATG	7 cut(s)	563, 748, 902, 965, 1112, 1270, 1477
HspAI	GCGC	4 cut(s)	830, 1074, 1076, 1514
KflI	GGGWCCC	1 cut(s)	502
KpnI	GGTACC	1 cut(s)	403
Ksp22I	TGATCA	1 cut(s)	874
Kzo9I	GATC	2 cut(s)	640, 874
LguI	GCTCTTC	1 cut(s)	50
LmnI	GCTCC	6 cut(s)	108, 123, 196, 399, 816, 936
Lsp1109I	GCAGC	3 cut(s)	72, 431, 1415
LweI	GCATC	2 cut(s)	384, 1489
MaeI	CTAG	1 cut(s)	933
MaeII	ACGT	2 cut(s)	535, 1293
MaeIII	GTNAC	5 cut(s)	155, 189, 1016, 1087, 1297
MalI	GATC	2 cut(s)	642, 876
MboI	GATC	2 cut(s)	640, 874
MhlI	GDGCHC	3 cut(s)	941, 1354, 1455
MlsI	TGGCCA	1 cut(s)	290
MluCI	AATT	2 cut(s)	604, 1634
MluNI	TGGCCA	1 cut(s)	290
MlyI	GAGTC	2 cut(s)	197, 218
MmeI	TCCRAC	4 cut(s)	252, 285, 375, 411
Mox20I	TGGCCA	1 cut(s)	290
MscI	TGGCCA	1 cut(s)	290
MseI	TTAA	6 cut(s)	164, 885, 1239, 1479, 1712, 1720
MslI	CAYNNNNRTG	1 cut(s)	558
Msp20I	TGGCCA	1 cut(s)	290
MspA1I	CMGCKG	1 cut(s)	422
MspCI	CTTAAG	1 cut(s)	1238
MspI	CCGG	4 cut(s)	14, 194, 397, 410
Mva1269I	GAATGC	2 cut(s)	1087, 1333
MvnI	CGCG	3 cut(s)	248, 1076, 1527
MwoI	GCNNNNNNNGC	6 cut(s)	353, 425, 731, 836, 1082, 1533
NdeII	GATC	2 cut(s)	640, 874
NlaIII	CATG	7 cut(s)	563, 748, 902, 965, 1112, 1270, 1477
NlaIV	GGNNCC	6 cut(s)	18, 104, 401, 503, 504, 1507
NmuCI	GTSAC	5 cut(s)	155, 189, 1016, 1087, 1297
NsbI	TGCGCA	1 cut(s)	831
NspI	RCATGY	2 cut(s)	1112, 1477
NspV	TTCGAA	1 cut(s)	609
PaeI	GCATGC	1 cut(s)	1112
PagI	TCATGA	1 cut(s)	559
PauI	GCGCGC	1 cut(s)	1074
PceI	AGGCCT	1 cut(s)	566
PciI	ACATGT	1 cut(s)	1473
PciSI	GCTCTTC	1 cut(s)	50
PcsI	WCGNNNNNNNCGW	2 cut(s)	270, 366
PctI	GAATGC	2 cut(s)	1087, 1333
PfeI	GAWTC	2 cut(s)	857, 1379
PflFI	GACNNNGTC	1 cut(s)	272
PkrI	GCNGC	4 cut(s)	62, 247, 421, 1430
PleI	GAGTC	2 cut(s)	196, 218
PpsI	GAGTC	2 cut(s)	196, 218
PpuMI	RGGWCCY	2 cut(s)	502, 1506
PscI	ACATGT	1 cut(s)	1473
Psp1406I	AACGTT	1 cut(s)	1293
Psp5II	RGGWCCY	2 cut(s)	502, 1506
PspFI	CCCAGC	2 cut(s)	207, 1502
PspN4I	GGNNCC	6 cut(s)	18, 104, 401, 503, 504, 1507
PspPI	GGNCC	3 cut(s)	16, 502, 1506
PspPPI	RGGWCCY	2 cut(s)	502, 1506
PstI	CTGCAG	1 cut(s)	1433
PsyI	GACNNNGTC	1 cut(s)	272
PteI	GCGCGC	1 cut(s)	1074
PvuII	CAGCTG	1 cut(s)	422
RsaI	GTAC	3 cut(s)	401, 864, 1524
RsaNI	GTAC	3 cut(s)	400, 863, 1523
RseI	CAYNNNNRTG	1 cut(s)	558
SalI	GTCGAC	1 cut(s)	598
SapI	GCTCTTC	1 cut(s)	50
SaqAI	TTAA	6 cut(s)	164, 885, 1239, 1479, 1712, 1720
SatI	GCNGC	4 cut(s)	61, 246, 420, 1429
Sau3AI	GATC	2 cut(s)	640, 874
Sau96I	GGNCC	3 cut(s)	16, 502, 1506
SchI	GAGTC	2 cut(s)	197, 218
SduI	GDGCHC	3 cut(s)	941, 1354, 1455
SfaNI	GCATC	2 cut(s)	384, 1489
SfcI	CTRYAG	1 cut(s)	1429
SfuI	TTCGAA	1 cut(s)	609
SinI	GGWCC	3 cut(s)	16, 502, 1506
SmiMI	CAYNNNNRTG	1 cut(s)	558
SmlI	CTYRAG	3 cut(s)	113, 1238, 1355
SmoI	CTYRAG	3 cut(s)	113, 1238, 1355
SphI	GCATGC	1 cut(s)	1112
Sse9I	AATT	2 cut(s)	604, 1634
SseBI	AGGCCT	1 cut(s)	566
SsiI	CCGC	4 cut(s)	243, 246, 1527, 1563
SspI	AATATT	1 cut(s)	889
SspMI	CTAG	1 cut(s)	933
StuI	AGGCCT	1 cut(s)	566
StyI	CCWWGG	2 cut(s)	1137, 1602
TaaI	ACNGT	2 cut(s)	881, 1099
TaiI	ACGT	2 cut(s)	538, 1296
TaqI	TCGA	7 cut(s)	222, 300, 369, 538, 599, 609, 729
TasI	AATT	2 cut(s)	604, 1634
TauI	GCSGC	1 cut(s)	248
TfiI	GAWTC	2 cut(s)	857, 1379
Tru1I	TTAA	6 cut(s)	164, 885, 1239, 1479, 1712, 1720
Tru9I	TTAA	6 cut(s)	164, 885, 1239, 1479, 1712, 1720
TscAI	CASTG	2 cut(s)	57, 1102
TseFI	GTSAC	5 cut(s)	155, 189, 1016, 1087, 1297
TseI	GCWGC	3 cut(s)	60, 419, 1428
Tsp45I	GTSAC	5 cut(s)	155, 189, 1016, 1087, 1297
TspDTI	ATGAA	5 cut(s)	16, 1056, 1073, 1178, 1460
TspRI	CASTG	2 cut(s)	57, 1102
Tth111I	GACNNNGTC	1 cut(s)	272
Vha464I	CTTAAG	1 cut(s)	1238
VpaK11BI	GGWCC	3 cut(s)	16, 502, 1506
XapI	RAATTY	1 cut(s)	604
XceI	RCATGY	2 cut(s)	1112, 1477
XmiI	GTMKAC	1 cut(s)	599
XspI	CTAG	1 cut(s)	933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.