MD13G1197300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006285
Physical Location & Seq
Reverse (-)
17262847 .. 17267827
4981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1197300.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGCTTCGAAGCTTTCAAAACCCCAATCTCTACTCCATTTCCAAAACCCACATCAAACTCCTGCATTACTTCTTATCCAATCACTTATGCACCACAACAGAGCCAACCCAAGTCCCCGAGTCACCTGACCTTCCGAACTGGGTTAAATTCTTGGACACCAAGCACCCAGAAGCCGCAGGTTTAGACGAAGATTTTATCATCCCTTCACTTGCCAATTGGGTCGAAGCCCCAATGCTCAAAGACCCCAAAAAAGCCGTCAAGCTCCCAGTTGTCGAGATCGCGGATACCGACATAGACATAATTAGTAGAGTTTTGAAAAACCAGCACCCGTCGCTGGAAAATGTAGCTCAAGCTTTAAGTGGGATTGTTTTGGATTTGTCAAACGGTTTGGTGGAACGGGTTTTGAGGAGGTTTAGCAATGAATGGGTTCCGGCTTTCGGGTTTTTCACTTGGGCAAAAATGCAAACAGGTTATAGGCATGCGCCGGAGGTGTACAATTTTATGGTTGACATCTTGGGGAAAGCCGAGAAGTTTGACATTATGTGGGATTTGGTTATAGAAATGGACAAAATTGGAGAAAGGTATGTTAGTTTGGATACGATGAGTAAGGTGATGAGGAGGCTTGCTAGGGCTGGTAGGTACAAGGAGGCAATCGATGCATTTCGAGGAATCGAGCAATTTGGAGTGAGCAAAGATGTGTTGTCTCTAAACGCTCTGATGGGTGCATTGGTGAAGGAGCGAAGCGTTGAGCTTGCCCACGAAATTTTCTTGGAGTTTAAGGAATCCATACCTTTGAATGTTAGTACTTTCAATGTTTTGATACATGGGTGGTGCAAATATAGGAACTTGGACATTGCTAGGAAGACAATGGAGGAGATGGAGAAGCACAGGTTTAAACCTGATGTTTTCTCGTATACCTCTTTGATTGAAGCTTATTGTAACGACAAAGATTTTCGCAAAGTTGCCGAAGTTCTTGGGGAGATGAAGGACAAGGGTTGTGAGCCAAATGCCGTGACTTATACCATCATAATGCATGCTTTAGGGAAGGCAAGGGAGATAACTAAGGCTTTGGATATTTACGAGAAGATGAAAATGAGTGGCTGTATTGCGGATACTTCATTTTACAGCTCGTTGATTTTCCTCCTTGGTAAAGCCGGTAGGCTTAAGGATGTTCAGGAAATAGTTGAGGACATGGAAAAGCAAGGTATAAAGCTGGACGTTTTGGCATATAATACCTTGATTAACATAGCATCTACGCATTCACAGGAAGAGGCTGCTCTCAAATGGCTCAAGAAAATGGAGGAGGATTTGTGCAAGCCGGATCTCAAGACCTATGCTCCGCTGTTGAAGTTGTTCTGCAGAAAGAAAAAGATGAGGGTGCCCTGCTTTTTGTTAGATCACATGTTTAAGAATGACATTGGCATTGAAGGTGGAACTTATGCACTCTTGGTACGCGGGCTGTGTATGAATGGTAAACTTGCAACCGCTTGCTCCTTCTTTGAGGAGATGGTATTGAAGGGTTTTTCTCCGAAGGACAGCACATACAGTATGTTGATAAAGGAATTGGAGGGTAAGAGTATGCAGAAAGAGAAGAAACATGTTGAGAGGTTGATGTTGCAGGCAAGAGGGCAAGACATAATAGGCAACGATTCTGCAGTGTCTACTGTTGAAGAGAGTAATGCACAAGGGGCATGTGTTTCTGTGATGTAA

Protein Analysis

570

Amino Acids

64.7

Weight (kDa)

6.7

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 263 - 294 6.5e-06 PPR repeat
PPR_2 PF13041 266 - 314 3.7e-14 PPR repeat family
TPR_24 PF23276 283 - 365 1.1e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 298 - 349 9.3e-14 Pentatricopeptide repeat domain
PPR_2 PF13041 300 - 349 1.2e-16 PPR repeat family
PPR PF01535 303 - 333 1.3e-06 PPR repeat
PPR_1 PF12854 331 - 364 8e-07 PPR repeat
PPR_2 PF13041 338 - 379 5.1e-07 PPR repeat family
PPR PF01535 338 - 368 4.2e-06 PPR repeat
TPR_24 PF23276 340 - 432 8.9e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 348 - 492 1.4e-14 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 371 - 415 1.2e-06 PPR repeat family
PPR_3 PF13812 394 - 452 3.6e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 406 - 454 3.5e-08 PPR repeat family
PPR_2 PF13041 479 - 522 2.7e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 167
AccB1I GGYRCC 1 cut(s) 1378
AccI GTMKAC 2 cut(s) 914, 1661
AccII CGCG 2 cut(s) 281, 1455
AciI CCGC 6 cut(s) 174, 281, 1109, 1340, 1455, 1485
AclWI GGATC 1 cut(s) 1329
AcsI RAATTY 2 cut(s) 146, 762
AfaI GTAC 4 cut(s) 494, 641, 805, 1452
AfiI CCNNNNNNNGG 2 cut(s) 334, 437
AflII CTTAAG 1 cut(s) 1163
AflIII ACRYGT 2 cut(s) 1401, 1597
AgsI TTSAA 9 cut(s) 17, 316, 796, 811, 929, 1348, 1427, 1516, 1670
AluBI AGCT 8 cut(s) 12, 262, 347, 353, 751, 932, 1128, 1213
AluI AGCT 8 cut(s) 12, 262, 347, 353, 751, 932, 1128, 1213
Alw26I GTCTC 1 cut(s) 708
AlwI GGATC 1 cut(s) 1329
Ama87I CYCGRG 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 1274
ApoI RAATTY 2 cut(s) 146, 762
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
Asp700I GAANNNNTTC 2 cut(s) 426, 1352
AspLEI GCGC 1 cut(s) 484
AsuHPI GGTGA 3 cut(s) 114, 622, 742
AsuII TTCGAA 1 cut(s) 7
AvaI CYCGRG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 1383
BaeI ACNNNNGTAYC 2 cut(s) 812, 845
BanI GGYRCC 1 cut(s) 1378
BbsI GAAGAC 1 cut(s) 869
BbvI GCAGC 1 cut(s) 1261
BccI CCATC 4 cut(s) 712, 871, 1031, 1501
BceAI ACGGC 2 cut(s) 239, 995
BciVI GTATCC 3 cut(s) 277, 589, 1105
BcoDI GTCTC 1 cut(s) 708
BfaI CTAG 2 cut(s) 627, 858
BfmI CTRYAG 2 cut(s) 1357, 1653
BfrI CTTAAG 1 cut(s) 1163
BfuAI ACCTGC 1 cut(s) 167
BfuI GTATCC 3 cut(s) 277, 589, 1105
BisI GCNGC 2 cut(s) 174, 1275
BlsI GCNGC 2 cut(s) 175, 1276
BmcAI AGTACT 1 cut(s) 805
BmeT110I CYCGRG 1 cut(s) 116
BmiI GGNNCC 2 cut(s) 429, 1380
BmrI ACTGGG 2 cut(s) 148, 260
BmsI GCATC 2 cut(s) 646, 1259
BmuI ACTGGG 2 cut(s) 148, 260
BpiI GAAGAC 1 cut(s) 869
Bpu14I TTCGAA 1 cut(s) 7
BpuEI CTTGAG 3 cut(s) 333, 1274, 1310
Bsa29I ATCGAT 1 cut(s) 654
BsaJI CCNNGG 1 cut(s) 1144
BsaXI ACNNNNNCTCC 4 cut(s) 1295, 1321, 1325, 1351
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 437
Bse118I RCCGGY 1 cut(s) 1154
Bse1I ACTGG 2 cut(s) 143, 266
Bse3DI GCAATG 2 cut(s) 424, 852
BseCI ATCGAT 1 cut(s) 654
BseDI CCNNGG 1 cut(s) 1144
BseGI GGATG 2 cut(s) 198, 1174
BseLI CCNNNNNNNGG 2 cut(s) 334, 437
BseMI GCAATG 2 cut(s) 424, 852
BseNI ACTGG 2 cut(s) 143, 266
BseRI GAGGAG 5 cut(s) 421, 631, 887, 1316, 1517
BseSI GKGCMC 1 cut(s) 1383
BseXI GCAGC 1 cut(s) 1261
Bsh1236I CGCG 2 cut(s) 281, 1455
BshNI GGYRCC 1 cut(s) 1378
BshVI ATCGAT 1 cut(s) 654
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 4 cut(s) 431, 485, 1155, 1319
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 2 cut(s) 334, 437
BsmAI GTCTC 1 cut(s) 708
BsmFI GGGAC 1 cut(s) 98
BsmI GAATGC 1 cut(s) 1258
BsoBI CYCGRG 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 1383
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 3 cut(s) 276, 1321, 1396
BspACI CCGC 6 cut(s) 174, 281, 1109, 1340, 1455, 1485
BspDI ATCGAT 1 cut(s) 654
BspFNI CGCG 2 cut(s) 281, 1455
BspLI GGNNCC 2 cut(s) 429, 1380
BspMAI CTGCAG 2 cut(s) 1361, 1657
BspMI ACCTGC 1 cut(s) 167
BspPI GGATC 1 cut(s) 1329
BspT104I TTCGAA 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 1378
BspTI CTTAAG 1 cut(s) 1163
BsrDI GCAATG 2 cut(s) 424, 852
BsrFI RCCGGY 1 cut(s) 1154
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 143, 266
BssAI RCCGGY 1 cut(s) 1154
BssECI CCNNGG 1 cut(s) 1144
BssMI GATC 3 cut(s) 276, 1321, 1396
BssNAI GTATAC 1 cut(s) 915
BssT1I CCWWGG 1 cut(s) 1144
Bst1107I GTATAC 1 cut(s) 915
Bst4CI ACNGT 3 cut(s) 386, 1547, 1666
Bst6I CTCTTC 2 cut(s) 1263, 1665
BstAFI CTTAAG 1 cut(s) 1163
BstAPI GCANNNNNTGC 1 cut(s) 656
BstAUI TGTACA 1 cut(s) 492
BstBI TTCGAA 1 cut(s) 7
BstC8I GCNNGC 8 cut(s) 480, 624, 753, 1035, 1316, 1457, 1489, 1620
BstDEI CTNAG 1 cut(s) 1062
BstF5I GGATG 2 cut(s) 198, 1174
BstFNI CGCG 2 cut(s) 281, 1455
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 3 cut(s) 279, 1324, 1399
BstMAI GTCTC 1 cut(s) 708
BstMBI GATC 3 cut(s) 276, 1321, 1396
BstMWI GCNNNNNNNGC 3 cut(s) 331, 656, 1688
BstNSI RCATGY 5 cut(s) 482, 1037, 1405, 1601, 1695
BstSFI CTRYAG 2 cut(s) 1357, 1653
BstSLI GKGCMC 1 cut(s) 1383
BstUI CGCG 2 cut(s) 281, 1455
BstV1I GCAGC 1 cut(s) 1261
BstV2I GAAGAC 1 cut(s) 869
BstX2I RGATCY 1 cut(s) 1321
BstYI RGATCY 1 cut(s) 1321
BstZ17I GTATAC 1 cut(s) 915
Bsu15I ATCGAT 1 cut(s) 654
BsuI GTATCC 3 cut(s) 277, 589, 1105
BsuTUI ATCGAT 1 cut(s) 654
BtsCI GGATG 2 cut(s) 198, 1174
BtsI GCAGTG 1 cut(s) 1662
BtsIMutI CAGTG 1 cut(s) 1662
BveI ACCTGC 1 cut(s) 167
Cac8I GCNNGC 8 cut(s) 480, 624, 753, 1035, 1316, 1457, 1489, 1620
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 1154
ClaI ATCGAT 1 cut(s) 654
Csp6I GTAC 4 cut(s) 493, 640, 804, 1451
CviAII CATG 7 cut(s) 479, 824, 1034, 1192, 1402, 1598, 1692
CviQI GTAC 4 cut(s) 493, 640, 804, 1451
DdeI CTNAG 1 cut(s) 1062
DpnI GATC 3 cut(s) 278, 1323, 1398
DpnII GATC 3 cut(s) 276, 1321, 1396
DraI TTTAAA 1 cut(s) 895
Eam1104I CTCTTC 2 cut(s) 1263, 1665
EarI CTCTTC 2 cut(s) 1263, 1665
Eco130I CCWWGG 1 cut(s) 1144
Eco88I CYCGRG 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 1144
EcoT22I ATGCAT 2 cut(s) 661, 1035
ErhI CCWWGG 1 cut(s) 1144
FaeI CATG 7 cut(s) 482, 827, 1037, 1195, 1405, 1601, 1695
FaqI GGGAC 1 cut(s) 98
FatI CATG 7 cut(s) 478, 823, 1033, 1191, 1401, 1597, 1691
FauI CCCGC 1 cut(s) 1448
FblI GTMKAC 2 cut(s) 914, 1661
Fnu4HI GCNGC 2 cut(s) 174, 1275
FokI GGATG 2 cut(s) 185, 1181
Fsp4HI GCNGC 2 cut(s) 174, 1275
FspBI CTAG 2 cut(s) 627, 858
GlaI GCGC 1 cut(s) 483
GluI GCNGC 2 cut(s) 174, 1275
HapII CCGG 4 cut(s) 431, 485, 1155, 1319
HhaI GCGC 1 cut(s) 484
Hin1II CATG 7 cut(s) 482, 827, 1037, 1195, 1405, 1601, 1695
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 3 cut(s) 10, 351, 930
HinfI GANTC 4 cut(s) 119, 669, 782, 1649
HpaII CCGG 4 cut(s) 431, 485, 1155, 1319
HphI GGTGA 3 cut(s) 114, 622, 742
Hpy166II GTNNAC 5 cut(s) 493, 508, 915, 1475, 1662
Hpy188I TCNGA 3 cut(s) 135, 717, 1530
Hpy188III TCNNGA 4 cut(s) 274, 1175, 1291, 1327
Hpy8I GTNNAC 5 cut(s) 493, 508, 915, 1475, 1662
Hpy99I CGWCG 1 cut(s) 334
HpyAV CCTTC 9 cut(s) 140, 213, 727, 979, 1039, 1421, 1504, 1510, 1525
HpyCH4III ACNGT 3 cut(s) 386, 1547, 1666
HpyCH4IV ACGT 1 cut(s) 1218
HpyF10VI GCNNNNNNNGC 3 cut(s) 331, 656, 1688
HpyF3I CTNAG 1 cut(s) 1062
HpySE526I ACGT 1 cut(s) 1218
Hsp92II CATG 7 cut(s) 482, 827, 1037, 1195, 1405, 1601, 1695
HspAI GCGC 1 cut(s) 482
Kzo9I GATC 3 cut(s) 276, 1321, 1396
LmnI GCTCC 4 cut(s) 267, 736, 1342, 1496
Lsp1109I GCAGC 1 cut(s) 1261
LweI GCATC 2 cut(s) 646, 1259
MaeI CTAG 2 cut(s) 627, 858
MaeII ACGT 1 cut(s) 1218
MaeIII GTNAC 3 cut(s) 120, 938, 1012
MalI GATC 3 cut(s) 278, 1323, 1398
MboI GATC 3 cut(s) 276, 1321, 1396
MboII GAAGA 6 cut(s) 200, 874, 1096, 1280, 1603, 1682
MfeI CAATTG 1 cut(s) 214
MflI RGATCY 1 cut(s) 1321
MhlI GDGCHC 1 cut(s) 1383
MluCI AATT 8 cut(s) 146, 214, 300, 496, 570, 677, 762, 1562
MlyI GAGTC 1 cut(s) 128
Mph1103I ATGCAT 2 cut(s) 661, 1035
MroXI GAANNNNTTC 2 cut(s) 426, 1352
MseI TTAA 7 cut(s) 144, 356, 777, 894, 1164, 1242, 1407
MslI CAYNNNNRTG 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 1342
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 4 cut(s) 431, 485, 1155, 1319
MssI GTTTAAAC 1 cut(s) 895
MunI CAATTG 1 cut(s) 214
Mva1269I GAATGC 1 cut(s) 1258
MvnI CGCG 2 cut(s) 281, 1455
MwoI GCNNNNNNNGC 3 cut(s) 331, 656, 1688
NdeII GATC 3 cut(s) 276, 1321, 1396
NlaIII CATG 7 cut(s) 482, 827, 1037, 1195, 1405, 1601, 1695
NlaIV GGNNCC 2 cut(s) 429, 1380
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 2 cut(s) 120, 1012
NsiI ATGCAT 2 cut(s) 661, 1035
NspI RCATGY 5 cut(s) 482, 1037, 1405, 1601, 1695
NspV TTCGAA 1 cut(s) 7
PaeI GCATGC 2 cut(s) 482, 1037
PciI ACATGT 2 cut(s) 1401, 1597
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 1258
PdmI GAANNNNTTC 2 cut(s) 426, 1352
PfeI GAWTC 3 cut(s) 669, 782, 1649
PkrI GCNGC 2 cut(s) 175, 1276
PleI GAGTC 1 cut(s) 127
PmeI GTTTAAAC 1 cut(s) 895
PpsI GAGTC 1 cut(s) 127
PscI ACATGT 2 cut(s) 1401, 1597
PspN4I GGNNCC 2 cut(s) 429, 1380
PstI CTGCAG 2 cut(s) 1361, 1657
PsuI RGATCY 1 cut(s) 1321
RsaI GTAC 4 cut(s) 494, 641, 805, 1452
RsaNI GTAC 4 cut(s) 493, 640, 804, 1451
RseI CAYNNNNRTG 1 cut(s) 1028
SaqAI TTAA 7 cut(s) 144, 356, 777, 894, 1164, 1242, 1407
SatI GCNGC 2 cut(s) 174, 1275
Sau3AI GATC 3 cut(s) 276, 1321, 1396
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 1 cut(s) 128
SduI GDGCHC 1 cut(s) 1383
SfaNI GCATC 2 cut(s) 646, 1259
SfcI CTRYAG 2 cut(s) 1357, 1653
SfuI TTCGAA 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 4 cut(s) 348, 1163, 1289, 1325
SmoI CTYRAG 4 cut(s) 348, 1163, 1289, 1325
SphI GCATGC 2 cut(s) 482, 1037
Sse9I AATT 8 cut(s) 146, 214, 300, 496, 570, 677, 762, 1562
SsiI CCGC 6 cut(s) 174, 281, 1109, 1340, 1455, 1485
SspMI CTAG 2 cut(s) 627, 858
StyI CCWWGG 1 cut(s) 1144
TaaI ACNGT 3 cut(s) 386, 1547, 1666
TaiI ACGT 1 cut(s) 1221
TaqI TCGA 6 cut(s) 7, 222, 273, 654, 664, 672
TasI AATT 8 cut(s) 146, 214, 300, 496, 570, 677, 762, 1562
TatI WGTACW 2 cut(s) 492, 803
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 3 cut(s) 669, 782, 1649
Tru1I TTAA 7 cut(s) 144, 356, 777, 894, 1164, 1242, 1407
Tru9I TTAA 7 cut(s) 144, 356, 777, 894, 1164, 1242, 1407
TscAI CASTG 1 cut(s) 1662
TseFI GTSAC 2 cut(s) 120, 1012
TseI GCWGC 1 cut(s) 1274
Tsp45I GTSAC 2 cut(s) 120, 1012
TspDTI ATGAA 5 cut(s) 435, 998, 1103, 1107, 1481
TspRI CASTG 1 cut(s) 1662
Vha464I CTTAAG 1 cut(s) 1163
XapI RAATTY 2 cut(s) 146, 762
XceI RCATGY 5 cut(s) 482, 1037, 1405, 1601, 1695
XmiI GTMKAC 2 cut(s) 914, 1661
XmnI GAANNNNTTC 2 cut(s) 426, 1352
XspI CTAG 2 cut(s) 627, 858
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 2 cut(s) 661, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.