Rh4CG014300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006285
Physical Location & Seq
Reverse (-)
2325959 .. 2327587
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG014300.1

Sequence Viewer

Length: 1629 bp
ATGCTCACAAGGCTCCACAATCTCAAGCTCCATTCAACTTCAAAAACCCACATCAATGCCCTGCGTTACTTCTTAAACCCTCTATGCACCACAACCGAGGTCGCCGAGTCACCGGACCTTCCAAGCTGGGTAAAGTTCTTCGACACCAACCACCCAAAACACGCAGCTTCCGACGACGACGACTTCGTCATCCCTTCACTGGCCAATTGGGTCGAAACCCACAACCTAAACCACCCCACCAAACCTCCCAAGCCCTCAACTTCCGATACCGACGACCTCGAAATCATCAGTAGAATCTTGAAAATCCGGTACCCATCGCCGGAAAATGCAGCTGAAACGTTGGAAAGGTACGATTTTGAGGTGTCAAACGGTTTGGTGCAACGGGTTTTGATGAGGTTTAGCAATGATTGGGTGCCGGCGTTGGGGTTTTTCATTTGGGCAAAACGTCGAACAGGTTATAGGCATCTTCCTGAGGCCTATAACTCCATGGTTGATGTATTGGGAAAGTCCAAGAATTTTGAACTTATGTGGGATTTTGTTGAGGAAATGGATCAAATTGGAGAGGGGTATGTGACGTTGGAGACAATGACTAAGGTGTTTAGGAGACTTGCAAAGGCTGGTAAGCACCAAGAGGCAATCGAGGCGTTTCGGGGCATGGAGCGGTTCGGATTGAGCAAAGATGTTGCGGCATTGAACACTCTGATGAATGCATTGGTGAAGGAGAGAAGCGTTGAGCTTGCCCAAGAAGCTTTCTTGGAGTTTAAGGAATCTATACCCGTGAATTCTCACAGCTTTAATGTTTTGATACATGGGTGGTGTAAGAATAGGAAGTTGGATATTGCTAGGAACACAATGGAGGAGATGGAGAGACACGGGTTTCGCCCGGATGTTTTCTCTTATACTTGTTTGGTTGAGGCGTTTTGTAGTGACAAGGATTTTCGCAAAGTTGATGAAGTTCTTAGTGAAATGAAGGGCAAGGCTTGCGAGCCGAATGCTGTGATATACACTATTGTAATGCATGCTTTGGGGAAGGCGAGGGAGATCACCAAGGCTTTGGAGGTTTATGAGTTGATGAAAGGGAATGGTGTTGTTCCTGATGTTTCGTTTTACAGCTCTTTGATTTACATTCTTGGTAAAGCCGGCAGGCTTAAGGATGTTCAAGAAGTGGTTGAGGACATGACAAAGCAGGGTGTAAAACCAGATGCTATGACCTATAACACCCTGATTTCTTGTGCTTGTGAGCATTCGCAGGAAGAGGCTGCACTCAAGTTGCTGAAGAAAATGGAGGAGGATTCGTGCAAGCCAGATGTTCAGACATACGCCCCGTTGTTGAAGATGTGCTGCAGAAAGAAGAGGATGAAGGTGCTCTACTTTTTGTTAGACCACATGTTTAAGAATGACATTAGTATCGACGCTGGAACCTATGCGCTCTTGGTACGTGGGATGTGCAAGAATGGTAAACTCGAAAACGCTTGCTCCTTCTTTGAGGAGATGGTATTGAAGAGTTTTGTTCCCAAGGACAGCACATACAAGATGTTGATAGAGGAATTGCAGGGGAAGAGTATGGAGAAAGAGAAGAAACATATTGAGGAGTTGATGTTGCAGGCAAAAGAGCAAGGTAACGTATAA

Protein Analysis

542

Amino Acids

62.13

Weight (kDa)

6.28

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 255 - 383 4.6e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 261 - 308 1.6e-13 PPR repeat family
PPR_3 PF13812 285 - 344 8e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 295 - 344 3.5e-11 PPR repeat family
TPR_24 PF23276 326 - 427 6.8e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 330 - 374 1.3e-08 PPR repeat family
PPR_long PF17177 343 - 514 1e-17 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 365 - 413 7.2e-11 PPR repeat family
TPR_24 PF23276 378 - 497 1.9e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 389 - 447 1.8e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 397 - 429 1.6e-07 PPR repeat
PPR_2 PF13041 400 - 449 7.5e-14 PPR repeat family
PPR_1 PF12854 468 - 499 1.3e-07 PPR repeat
PPR_2 PF13041 472 - 516 7.5e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 185
Acc65I GGTACC 1 cut(s) 309
AccB1I GGYRCC 2 cut(s) 309, 412
AccBSI CCGCTC 1 cut(s) 661
AciI CCGC 2 cut(s) 661, 686
AclI AACGTT 1 cut(s) 338
AclWI GGATC 1 cut(s) 558
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 2 cut(s) 514, 781
AcuI CTGAAG 1 cut(s) 1295
AfaI GTAC 3 cut(s) 311, 350, 1437
AfiI CCNNNNNNNGG 3 cut(s) 199, 319, 422
AflII CTTAAG 1 cut(s) 1148
AflIII ACRYGT 1 cut(s) 1386
AgsI TTSAA 8 cut(s) 36, 42, 301, 521, 694, 1160, 1333, 1501
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
AluBI AGCT 8 cut(s) 28, 126, 167, 332, 736, 749, 792, 1113
AluI AGCT 8 cut(s) 28, 126, 167, 332, 736, 749, 792, 1113
Alw21I GWGCWC 1 cut(s) 1368
Alw26I GTCTC 3 cut(s) 575, 598, 862
AlwI GGATC 1 cut(s) 558
AoxI GGCC 2 cut(s) 201, 474
ApeKI GCWGC 4 cut(s) 164, 329, 1259, 1341
ApoI RAATTY 2 cut(s) 514, 781
ArsI GACNNNNNNTTYG 4 cut(s) 167, 199, 861, 893
Asp718I GGTACC 1 cut(s) 309
AspLEI GCGC 1 cut(s) 1429
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 1 cut(s) 884
AsuHPI GGTGA 3 cut(s) 102, 727, 1036
AvaII GGWCC 1 cut(s) 115
AxyI CCTNAGG 1 cut(s) 471
BaeI ACNNNNGTAYC 4 cut(s) 797, 830, 1390, 1423
BalI TGGCCA 1 cut(s) 203
BanI GGYRCC 2 cut(s) 309, 412
BarI GAAGNNNNNNTAC 2 cut(s) 1352, 1384
Bbv12I GWGCWC 1 cut(s) 1368
BbvI GCAGC 4 cut(s) 176, 341, 1246, 1328
BccI CCATC 3 cut(s) 322, 856, 1486
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BcnI CCSGG 1 cut(s) 884
BcoDI GTCTC 3 cut(s) 575, 598, 862
BfaI CTAG 1 cut(s) 843
BfmI CTRYAG 1 cut(s) 1342
BfrI CTTAAG 1 cut(s) 1148
BisI GCNGC 5 cut(s) 165, 330, 687, 1260, 1342
BlsI GCNGC 5 cut(s) 166, 331, 688, 1261, 1343
Bme1390I CCNGG 1 cut(s) 884
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 4 cut(s) 14, 311, 414, 1420
BmrFI CCNGG 1 cut(s) 884
BmsI GCATC 2 cut(s) 472, 1192
BpuEI CTTGAG 2 cut(s) 8, 1250
BpuMI CCSGG 1 cut(s) 884
BsaAI YACGTR 1 cut(s) 1439
BsaJI CCNNGG 4 cut(s) 96, 486, 1047, 1515
BsaWI WCCGGW 2 cut(s) 112, 306
BsaXI ACNNNNNCTCC 8 cut(s) 229, 259, 1280, 1310, 1460, 1490, 1583, 1613
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 319, 422
Bse118I RCCGGY 2 cut(s) 415, 1139
Bse1I ACTGG 1 cut(s) 204
Bse21I CCTNAGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 409
BseDI CCNNGG 4 cut(s) 96, 486, 1047, 1515
BseGI GGATG 5 cut(s) 189, 892, 1159, 1362, 1449
BseLI CCNNNNNNNGG 3 cut(s) 199, 319, 422
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 4 cut(s) 872, 1301, 1502, 1604
BseXI GCAGC 4 cut(s) 176, 341, 1246, 1328
BseYI CCCAGC 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 1245
BshFI GGCC 2 cut(s) 203, 476
BshNI GGYRCC 2 cut(s) 309, 412
BsiHKAI GWGCWC 1 cut(s) 1368
BsiSI CCGG 6 cut(s) 113, 307, 320, 416, 884, 1140
BslI CCNNNNNNNGG 3 cut(s) 199, 319, 422
BsmAI GTCTC 3 cut(s) 575, 598, 862
BsmI GAATGC 3 cut(s) 712, 997, 1243
BsnI GGCC 2 cut(s) 203, 476
Bsp1286I GDGCHC 1 cut(s) 1368
Bsp143I GATC 2 cut(s) 550, 1041
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 2 cut(s) 661, 686
BspANI GGCC 2 cut(s) 203, 476
BspCNI CTCAG 1 cut(s) 463
BspLI GGNNCC 4 cut(s) 14, 311, 414, 1420
BspMAI CTGCAG 1 cut(s) 1346
BspPI GGATC 1 cut(s) 558
BspT107I GGYRCC 2 cut(s) 309, 412
BspTI CTTAAG 1 cut(s) 1148
BsrBI CCGCTC 1 cut(s) 661
BsrDI GCAATG 1 cut(s) 409
BsrFI RCCGGY 2 cut(s) 415, 1139
BsrI ACTGG 1 cut(s) 204
BssAI RCCGGY 2 cut(s) 415, 1139
BssECI CCNNGG 4 cut(s) 96, 486, 1047, 1515
BssMI GATC 2 cut(s) 550, 1041
BssT1I CCWWGG 3 cut(s) 486, 1047, 1515
Bst4CI ACNGT 1 cut(s) 371
Bst6I CTCTTC 4 cut(s) 1248, 1346, 1496, 1553
BstAFI CTTAAG 1 cut(s) 1148
BstAPI GCANNNNNTGC 1 cut(s) 981
BstBAI YACGTR 1 cut(s) 1439
BstDEI CTNAG 3 cut(s) 471, 591, 959
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 5 cut(s) 189, 892, 1159, 1362, 1449
BstHHI GCGC 1 cut(s) 1429
BstKTI GATC 2 cut(s) 553, 1044
BstMAI GTCTC 3 cut(s) 575, 598, 862
BstMBI GATC 2 cut(s) 550, 1041
BstMWI GCNNNNNNNGC 4 cut(s) 10, 641, 746, 981
BstNSI RCATGY 2 cut(s) 1022, 1390
BstSCI CCNGG 1 cut(s) 882
BstSFI CTRYAG 1 cut(s) 1342
BstV1I GCAGC 4 cut(s) 176, 341, 1246, 1328
BstXI CCANNNNNNTGG 1 cut(s) 1054
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 2 cut(s) 203, 476
BtgI CCRYGG 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 5 cut(s) 189, 892, 1159, 1362, 1449
BtsIMutI CAGTG 1 cut(s) 197
CfoI GCGC 1 cut(s) 1429
Cfr10I RCCGGY 2 cut(s) 415, 1139
Cfr13I GGNCC 1 cut(s) 115
CseI GACGC 1 cut(s) 1421
Csp6I GTAC 3 cut(s) 310, 349, 1436
CviAII CATG 6 cut(s) 487, 655, 809, 1019, 1177, 1387
CviQI GTAC 3 cut(s) 310, 349, 1436
DdeI CTNAG 3 cut(s) 471, 591, 959
DpnI GATC 2 cut(s) 552, 1043
DpnII GATC 2 cut(s) 550, 1041
DrdI GACNNNNNNGTC 1 cut(s) 185
DseDI GACNNNNNNGTC 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 4 cut(s) 1248, 1346, 1496, 1553
EarI CTCTTC 4 cut(s) 1248, 1346, 1496, 1553
Eco130I CCWWGG 3 cut(s) 486, 1047, 1515
Eco147I AGGCCT 1 cut(s) 476
Eco47I GGWCC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 1295
Eco81I CCTNAGG 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 781
EcoT14I CCWWGG 3 cut(s) 486, 1047, 1515
EcoT22I ATGCAT 2 cut(s) 712, 1020
ErhI CCWWGG 3 cut(s) 486, 1047, 1515
FaeI CATG 6 cut(s) 490, 658, 812, 1022, 1180, 1390
FatI CATG 6 cut(s) 486, 654, 808, 1018, 1176, 1386
Fnu4HI GCNGC 5 cut(s) 165, 330, 687, 1260, 1342
FokI GGATG 5 cut(s) 176, 899, 1166, 1369, 1456
Fsp4HI GCNGC 5 cut(s) 165, 330, 687, 1260, 1342
FspBI CTAG 1 cut(s) 843
GlaI GCGC 1 cut(s) 1428
GluI GCNGC 5 cut(s) 165, 330, 687, 1260, 1342
GsaI CCCAGC 1 cut(s) 130
HaeIII GGCC 2 cut(s) 203, 476
HapII CCGG 6 cut(s) 113, 307, 320, 416, 884, 1140
HgaI GACGC 1 cut(s) 1421
HhaI GCGC 1 cut(s) 1429
Hin1II CATG 6 cut(s) 490, 658, 812, 1022, 1180, 1390
Hin6I GCGC 1 cut(s) 1427
HinP1I GCGC 1 cut(s) 1427
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 4 cut(s) 107, 294, 767, 1292
HpaII CCGG 6 cut(s) 113, 307, 320, 416, 884, 1140
HphI GGTGA 3 cut(s) 102, 727, 1036
Hpy166II GTNNAC 1 cut(s) 1460
Hpy188I TCNGA 5 cut(s) 172, 265, 668, 702, 1314
Hpy188III TCNNGA 4 cut(s) 298, 470, 1094, 1160
Hpy8I GTNNAC 1 cut(s) 1460
Hpy99I CGWCG 6 cut(s) 176, 179, 182, 275, 450, 1415
HpyAV CCTTC 7 cut(s) 128, 204, 712, 964, 1024, 1354, 1489
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4IV ACGT 5 cut(s) 338, 445, 575, 1438, 1623
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 641, 746, 981
HpyF3I CTNAG 3 cut(s) 471, 591, 959
HpySE526I ACGT 5 cut(s) 338, 445, 575, 1438, 1623
Hsp92II CATG 6 cut(s) 490, 658, 812, 1022, 1180, 1390
HspAI GCGC 1 cut(s) 1427
KpnI GGTACC 1 cut(s) 313
KroI GCCGGC 2 cut(s) 415, 1139
KroNI GCCGGC 2 cut(s) 417, 1141
Kzo9I GATC 2 cut(s) 550, 1041
LmnI GCTCC 4 cut(s) 18, 33, 658, 1481
Lsp1109I GCAGC 4 cut(s) 176, 341, 1246, 1328
LweI GCATC 2 cut(s) 472, 1192
MaeI CTAG 1 cut(s) 843
MaeII ACGT 5 cut(s) 338, 445, 575, 1438, 1623
MaeIII GTNAC 5 cut(s) 65, 108, 571, 926, 1619
MalI GATC 2 cut(s) 552, 1043
MbiI CCGCTC 1 cut(s) 661
MboI GATC 2 cut(s) 550, 1041
MboII GAAGA 9 cut(s) 130, 458, 1265, 1288, 1345, 1363, 1513, 1570, 1588
MfeI CAATTG 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 1368
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 5 cut(s) 205, 514, 555, 781, 1547
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 4 cut(s) 195, 321, 558, 813
Mox20I TGGCCA 1 cut(s) 203
Mph1103I ATGCAT 2 cut(s) 712, 1020
MroNI GCCGGC 2 cut(s) 415, 1139
MscI TGGCCA 1 cut(s) 203
MseI TTAA 5 cut(s) 74, 762, 795, 1149, 1392
MslI CAYNNNNRTG 2 cut(s) 54, 701
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 332
MspCI CTTAAG 1 cut(s) 1148
MspI CCGG 6 cut(s) 113, 307, 320, 416, 884, 1140
MspR9I CCNGG 1 cut(s) 884
MunI CAATTG 1 cut(s) 205
Mva1269I GAATGC 3 cut(s) 712, 997, 1243
MwoI GCNNNNNNNGC 4 cut(s) 10, 641, 746, 981
NaeI GCCGGC 2 cut(s) 417, 1141
NciI CCSGG 1 cut(s) 884
NcoI CCATGG 1 cut(s) 486
NdeII GATC 2 cut(s) 550, 1041
NgoMIV GCCGGC 2 cut(s) 415, 1139
NlaIII CATG 6 cut(s) 490, 658, 812, 1022, 1180, 1390
NlaIV GGNNCC 4 cut(s) 14, 311, 414, 1420
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 3 cut(s) 108, 571, 926
NsiI ATGCAT 2 cut(s) 712, 1020
NspI RCATGY 2 cut(s) 1022, 1390
PaeI GCATGC 1 cut(s) 1022
PceI AGGCCT 1 cut(s) 476
PciI ACATGT 1 cut(s) 1386
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 183
PctI GAATGC 3 cut(s) 712, 997, 1243
PdiI GCCGGC 2 cut(s) 417, 1141
PfeI GAWTC 3 cut(s) 294, 767, 1292
PflFI GACNNNGTC 1 cut(s) 185
PkrI GCNGC 5 cut(s) 166, 331, 688, 1261, 1343
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 1439
PscI ACATGT 1 cut(s) 1386
Psp1406I AACGTT 1 cut(s) 338
PspFI CCCAGC 1 cut(s) 126
PspN4I GGNNCC 4 cut(s) 14, 311, 414, 1420
PspPI GGNCC 1 cut(s) 115
PstI CTGCAG 1 cut(s) 1346
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 3 cut(s) 311, 350, 1437
RsaNI GTAC 3 cut(s) 310, 349, 1436
RseI CAYNNNNRTG 2 cut(s) 54, 701
SaqAI TTAA 5 cut(s) 74, 762, 795, 1149, 1392
SatI GCNGC 5 cut(s) 165, 330, 687, 1260, 1342
Sau3AI GATC 2 cut(s) 550, 1041
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 884
SduI GDGCHC 1 cut(s) 1368
SfaNI GCATC 2 cut(s) 472, 1192
SfcI CTRYAG 1 cut(s) 1342
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 2 cut(s) 54, 701
SmlI CTYRAG 3 cut(s) 23, 1148, 1265
SmoI CTYRAG 3 cut(s) 23, 1148, 1265
SphI GCATGC 1 cut(s) 1022
Sse9I AATT 5 cut(s) 205, 514, 555, 781, 1547
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 2 cut(s) 661, 686
SspMI CTAG 1 cut(s) 843
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 1 cut(s) 882
StyI CCWWGG 3 cut(s) 486, 1047, 1515
TaaI ACNGT 1 cut(s) 371
TaiI ACGT 5 cut(s) 341, 448, 578, 1441, 1626
TaqI TCGA 7 cut(s) 141, 213, 279, 448, 639, 1410, 1464
TasI AATT 5 cut(s) 205, 514, 555, 781, 1547
TauI GCSGC 1 cut(s) 689
TfiI GAWTC 3 cut(s) 294, 767, 1292
Tru1I TTAA 5 cut(s) 74, 762, 795, 1149, 1392
Tru9I TTAA 5 cut(s) 74, 762, 795, 1149, 1392
TscAI CASTG 1 cut(s) 204
TseFI GTSAC 3 cut(s) 108, 571, 926
TseI GCWGC 4 cut(s) 164, 329, 1259, 1341
Tsp45I GTSAC 3 cut(s) 108, 571, 926
TspDTI ATGAA 6 cut(s) 421, 719, 966, 983, 1088, 1373
TspRI CASTG 1 cut(s) 204
Tth111I GACNNNGTC 1 cut(s) 185
Vha464I CTTAAG 1 cut(s) 1148
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 2 cut(s) 514, 781
XceI RCATGY 2 cut(s) 1022, 1390
XspI CTAG 1 cut(s) 843
Zsp2I ATGCAT 2 cut(s) 712, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.