Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0006285

Physical Location & Seq

Genomic Coordinates Reverse (-)

1266338 .. 1267969

Gene Structure 1632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG009300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGCTCACAAGGCTCCACAATCTCAAGCTCCATTCAACTTCAAAAACCCACATCAATGCCCTGCGTTACTTCTTAAACCCTCTATGCACCACAACCGAGGTCGCCGAGTCACCCGAGCTTCCAAGCTGGGTCAAGTTCTTCGACACCAACCACCCAAAACACGCAGCTCCCGACGAAGACGACGACTTCGTCATCCCTTCACTGGCCAATTGGGTCGAAACCCACAACCTAAACCACCCCACCAAACCTCCCAAGCCCTCAACTTCCGATACCGACGACCTCGAAAGCATCAGTAGAATCTTGAAAACCCGGTACCCATCGCCGGAAAATGCAGCTGAAGCGTTGGAAGGGTACGATTTTGAGCTGTCAAACGGTTTGGTGCAACGGGTTTTGATGAGGTTTAGCAATGATTGGGTGCCGGCGTTGGGGTTTTTCATTTGGGCAAAACGTCGAACAGGTTATAGGCATCTCCCTGAGGCCTATAACTCCATGGTTGATGTATTGGGAAAGTCCAAGAATTTTGACCTTATGTGGGATTTTGTTGAGGAAATGGATCAAATTGGAGATGGGTATGTGACGTTGGAGACCATGACAAAGGTGTTTAGGAGACTTGCAAAGGCTGGTAAGCACCAAGAGGCAATCGAGGCGTTTCGGGGCATGGAGCGGTTCGGATTGAGCAAAGATGTTGCGGCATTGAACACTCTGATGAATGCATTGGTGAAGGAGAGAAGCGTTGAGCTTGCCCAAGAAGCTTTCTTGGAGTTTAAGGAATCTATACCTGTGAATTCTCACAGCTTTAATGTTTTGATACATGGGTGGTGTAAGAATAGGAAGTTGGATATTGCTAGGAACACAATGGAGGAGATGGAGAGACACGGGTTTCGCCGTGATGTTTTCTCTTATACTTGTTTGGTTGAGGCCTATTGTAGTGACAAGGATTTTCGCAAAGTTGATGAAGTTCTTAGTGAAATGAAGGGCAAGGCTTGCGAGCCGAATGCTGTGACATACACTATCGTAATGCATGCTTTGGGGAAGGCGAGGGAGATAACCAAGGCTTTGGAGGTTTATGAGTTGATGAAAGGGAATGGTGTTGTTCCTGATGTTTCGTTTTACAGCTCTTTGATTTACATTCTTGGTAAAGCCGGCAGGCTTAAGGATGTTCAAGAAGTGGTTGAGGATATGACAAAGCAGGGTGTAAAACCGGACGCTATGACCTATAACACCCTGATTTCTTGTGCTTGCGAGCATTCGCAGGAAGAGGCTGCTCTCAAGTTGCTGAAGAAAATGGAGGAGGATTCATGCAAGCCGGATGTTCAGACATACGCCCCATTGTTGAAGATGTGCTGCAGAAAGAAGAGGATGAAGGTGCTCTACTTTTTGTTAGACCACATGTTTAAGAATGACATTAGTATCGACGCTGGAACCTATGCGCTCTTGGTACGTGGGATGTGCAAGAATGGTAAACCCGAAAACGCTTGCTCCTTCTTTGAGGAGATGGCATTGAAGGGTTTTGTTCCCAAGGACAGTACATACAAGATGTTGCTAGAGGAATTGCAGGGGAAGAATATGGAGAAAGAGAAGAAACATATTGAGAAGTTGATGTTGCAGGCAAAAGAGCAAGGAAACGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

543

Amino Acids

62.12

Weight (kDa)

6.38

Isoelectric Point (pI)

37.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	256 - 384	3.4e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	262 - 309	1.6e-11	PPR repeat family
PPR_3	PF13812	286 - 345	2.2e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	296 - 345	4.5e-14	PPR repeat family
PPR_2	PF13041	334 - 375	1.1e-07	PPR repeat family
PPR	PF01535	334 - 364	7.4e-06	PPR repeat
TPR_24	PF23276	335 - 428	1.3e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	366 - 414	7.3e-11	PPR repeat family
PPR_long	PF17177	371 - 515	1.9e-12	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	390 - 448	1.8e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	398 - 430	1.6e-07	PPR repeat
PPR_2	PF13041	401 - 450	7.5e-14	PPR repeat family
PPR_1	PF12854	469 - 499	3.1e-06	PPR repeat
PPR_2	PF13041	473 - 517	9.4e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22670
fragaria_vesca	FvH4_4g00721
malus_domestica	MD13G1197300.v1.1
prunus_persica	Prupe.1G018400_v2.0.a1
pyrus_communis	pycom13g17020
rosa_chinensis	RchiOBHm_Chr1g0322101 RchiOBHm_Chr4g0386451
rosa_laevigata	RLG00000010131 RLG00000010157 RLG00000010161
rosa_multiflora	Rmu_co8274927.1_g000001 Rmu_sc0001942.1_g000008 Rmu_sc0001942.1_g000027 Rmu_sc0001942.1_g000051 Rmu_sc0002785.1_g000008 Rmu_sc0003786.1_g000023 Rmu_sc0006648.1_g000016 Rmu_sc0036937.1_g000001 Rmu_ssc0000272.1_g000008 Rmu_ssc0000272.1_g000009
rosa_roxburghii	Rroxscaffold_5G00333960 Rroxscaffold_6G00392780
rosa_samantha	Rh1AG049600 Rh4BG009300 Rh4CG014300 Rh4DG010100
rosa_wichuraiana	Rw4G000030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	188
Acc65I	GGTACC	1 cut(s)	312
AccB1I	GGYRCC	2 cut(s)	312, 415
AccBSI	CCGCTC	1 cut(s)	664
AciI	CCGC	2 cut(s)	664, 689
AclWI	GGATC	1 cut(s)	561
AcoI	YGGCCR	1 cut(s)	204
AcsI	RAATTY	2 cut(s)	517, 784
AcuI	CTGAAG	2 cut(s)	357, 1298
AfaI	GTAC	4 cut(s)	314, 353, 1440, 1528
AfiI	CCNNNNNNNGG	4 cut(s)	202, 322, 425, 532
AflII	CTTAAG	1 cut(s)	1151
AflIII	ACRYGT	1 cut(s)	1389
AgsI	TTSAA	7 cut(s)	36, 42, 304, 697, 1163, 1336, 1504
AjuI	GAANNNNNNNTTGG	2 cut(s)	818, 850
Alw21I	GWGCWC	1 cut(s)	1371
Alw26I	GTCTC	3 cut(s)	578, 601, 865
AlwI	GGATC	1 cut(s)	561
Ama87I	CYCGRG	1 cut(s)	113
AoxI	GGCC	3 cut(s)	204, 477, 918
ApeKI	GCWGC	4 cut(s)	164, 332, 1262, 1344
ApoI	RAATTY	2 cut(s)	517, 784
ArsI	GACNNNNNNTTYG	4 cut(s)	170, 202, 864, 896
Asp718I	GGTACC	1 cut(s)	312
AspLEI	GCGC	1 cut(s)	1432
AsuC2I	CCSGG	1 cut(s)	310
AsuHPI	GGTGA	2 cut(s)	102, 730
AvaI	CYCGRG	1 cut(s)	113
AxyI	CCTNAGG	1 cut(s)	474
BaeI	ACNNNNGTAYC	4 cut(s)	800, 833, 1393, 1426
BalI	TGGCCA	1 cut(s)	206
BanI	GGYRCC	2 cut(s)	312, 415
BarI	GAAGNNNNNNTAC	2 cut(s)	1355, 1387
BbsI	GAAGAC	1 cut(s)	183
Bbv12I	GWGCWC	1 cut(s)	1371
BbvI	GCAGC	4 cut(s)	176, 344, 1249, 1331
BccI	CCATC	4 cut(s)	325, 560, 859, 1489
BceAI	ACGGC	1 cut(s)	870
BcgI	CGANNNNNNTGC	2 cut(s)	977, 1011
BcnI	CCSGG	1 cut(s)	310
BcoDI	GTCTC	3 cut(s)	578, 601, 865
BfaI	CTAG	2 cut(s)	846, 1544
BfmI	CTRYAG	1 cut(s)	1345
BfrI	CTTAAG	1 cut(s)	1151
BisI	GCNGC	5 cut(s)	165, 333, 690, 1263, 1345
BlsI	GCNGC	5 cut(s)	166, 334, 691, 1264, 1346
Bme1390I	CCNGG	1 cut(s)	310
BmeT110I	CYCGRG	1 cut(s)	113
BmiI	GGNNCC	4 cut(s)	14, 314, 417, 1423
BmrFI	CCNGG	1 cut(s)	310
BmsI	GCATC	2 cut(s)	297, 475
BpiI	GAAGAC	1 cut(s)	183
BpuEI	CTTGAG	2 cut(s)	8, 1253
BpuMI	CCSGG	1 cut(s)	310
BsaAI	YACGTR	1 cut(s)	1442
BsaI	GGTCTC	1 cut(s)	578
BsaJI	CCNNGG	4 cut(s)	96, 489, 1050, 1518
BsaWI	WCCGGW	1 cut(s)	1201
BsaXI	ACNNNNNCTCC	4 cut(s)	232, 262, 1463, 1493
Bsc4I	CCNNNNNNNGG	4 cut(s)	202, 322, 425, 532
Bse118I	RCCGGY	2 cut(s)	418, 1142
Bse1I	ACTGG	1 cut(s)	207
Bse21I	CCTNAGG	1 cut(s)	474
Bse3DI	GCAATG	1 cut(s)	412
BseDI	CCNNGG	4 cut(s)	96, 489, 1050, 1518
BseGI	GGATG	5 cut(s)	192, 1162, 1315, 1365, 1452
BseLI	CCNNNNNNNGG	4 cut(s)	202, 322, 425, 532
BseMI	GCAATG	1 cut(s)	412
BseMII	CTCAG	1 cut(s)	465
BseNI	ACTGG	1 cut(s)	207
BseRI	GAGGAG	3 cut(s)	875, 1304, 1505
BseXI	GCAGC	4 cut(s)	176, 344, 1249, 1331
BseYI	CCCAGC	1 cut(s)	126
BshFI	GGCC	3 cut(s)	206, 479, 920
BshNI	GGYRCC	2 cut(s)	312, 415
BsiHKAI	GWGCWC	1 cut(s)	1371
BsiHKCI	CYCGRG	1 cut(s)	113
BsiSI	CCGG	6 cut(s)	310, 323, 419, 1143, 1202, 1307
BslI	CCNNNNNNNGG	4 cut(s)	202, 322, 425, 532
BsmAI	GTCTC	3 cut(s)	578, 601, 865
BsmI	GAATGC	3 cut(s)	715, 1000, 1246
BsnI	GGCC	3 cut(s)	206, 479, 920
Bso31I	GGTCTC	1 cut(s)	578
BsoBI	CYCGRG	1 cut(s)	113
Bsp1286I	GDGCHC	1 cut(s)	1371
Bsp143I	GATC	1 cut(s)	553
Bsp19I	CCATGG	1 cut(s)	489
BspACI	CCGC	2 cut(s)	664, 689
BspANI	GGCC	3 cut(s)	206, 479, 920
BspCNI	CTCAG	1 cut(s)	466
BspLI	GGNNCC	4 cut(s)	14, 314, 417, 1423
BspMAI	CTGCAG	1 cut(s)	1349
BspPI	GGATC	1 cut(s)	561
BspT107I	GGYRCC	2 cut(s)	312, 415
BspTI	CTTAAG	1 cut(s)	1151
BspTNI	GGTCTC	1 cut(s)	578
BsrBI	CCGCTC	1 cut(s)	664
BsrDI	GCAATG	1 cut(s)	412
BsrFI	RCCGGY	2 cut(s)	418, 1142
BsrI	ACTGG	1 cut(s)	207
BssAI	RCCGGY	2 cut(s)	418, 1142
BssECI	CCNNGG	4 cut(s)	96, 489, 1050, 1518
BssMI	GATC	1 cut(s)	553
BssT1I	CCWWGG	3 cut(s)	489, 1050, 1518
Bst4CI	ACNGT	2 cut(s)	374, 1526
Bst6I	CTCTTC	2 cut(s)	1251, 1349
BstAFI	CTTAAG	1 cut(s)	1151
BstAPI	GCANNNNNTGC	1 cut(s)	984
BstBAI	YACGTR	1 cut(s)	1442
BstDEI	CTNAG	2 cut(s)	474, 962
BstDSI	CCRYGG	1 cut(s)	489
BstF5I	GGATG	5 cut(s)	192, 1162, 1315, 1365, 1452
BstHHI	GCGC	1 cut(s)	1432
BstKTI	GATC	1 cut(s)	556
BstMAI	GTCTC	3 cut(s)	578, 601, 865
BstMBI	GATC	1 cut(s)	553
BstMWI	GCNNNNNNNGC	5 cut(s)	10, 338, 644, 749, 984
BstNSI	RCATGY	2 cut(s)	1025, 1393
BstSCI	CCNGG	1 cut(s)	308
BstSFI	CTRYAG	1 cut(s)	1345
BstV1I	GCAGC	4 cut(s)	176, 344, 1249, 1331
BstV2I	GAAGAC	1 cut(s)	183
BstXI	CCANNNNNNTGG	1 cut(s)	1057
Bsu36I	CCTNAGG	1 cut(s)	474
BsuRI	GGCC	3 cut(s)	206, 479, 920
BtgI	CCRYGG	1 cut(s)	489
BtgZI	GCGATG	1 cut(s)	303
BtsCI	GGATG	5 cut(s)	192, 1162, 1315, 1365, 1452
BtsIMutI	CAGTG	1 cut(s)	200
CfoI	GCGC	1 cut(s)	1432
Cfr10I	RCCGGY	2 cut(s)	418, 1142
CseI	GACGC	2 cut(s)	1214, 1424
Csp6I	GTAC	4 cut(s)	313, 352, 1439, 1527
CviAII	CATG	7 cut(s)	490, 589, 658, 812, 1022, 1299, 1390
CviQI	GTAC	4 cut(s)	313, 352, 1439, 1527
DdeI	CTNAG	2 cut(s)	474, 962
DpnI	GATC	1 cut(s)	555
DpnII	GATC	1 cut(s)	553
DrdI	GACNNNNNNGTC	1 cut(s)	188
DseDI	GACNNNNNNGTC	1 cut(s)	188
EaeI	YGGCCR	1 cut(s)	204
Eam1104I	CTCTTC	2 cut(s)	1251, 1349
EarI	CTCTTC	2 cut(s)	1251, 1349
Eco130I	CCWWGG	3 cut(s)	489, 1050, 1518
Eco147I	AGGCCT	2 cut(s)	479, 920
Eco31I	GGTCTC	1 cut(s)	578
Eco57I	CTGAAG	2 cut(s)	357, 1298
Eco81I	CCTNAGG	1 cut(s)	474
Eco88I	CYCGRG	1 cut(s)	113
EcoRI	GAATTC	1 cut(s)	784
EcoT14I	CCWWGG	3 cut(s)	489, 1050, 1518
EcoT22I	ATGCAT	2 cut(s)	715, 1023
ErhI	CCWWGG	3 cut(s)	489, 1050, 1518
FaeI	CATG	7 cut(s)	493, 592, 661, 815, 1025, 1302, 1393
FatI	CATG	7 cut(s)	489, 588, 657, 811, 1021, 1298, 1389
Fnu4HI	GCNGC	5 cut(s)	165, 333, 690, 1263, 1345
FokI	GGATG	5 cut(s)	179, 1169, 1322, 1372, 1459
Fsp4HI	GCNGC	5 cut(s)	165, 333, 690, 1263, 1345
FspBI	CTAG	2 cut(s)	846, 1544
GlaI	GCGC	1 cut(s)	1431
GluI	GCNGC	5 cut(s)	165, 333, 690, 1263, 1345
GsaI	CCCAGC	1 cut(s)	130
HaeIII	GGCC	3 cut(s)	206, 479, 920
HapII	CCGG	6 cut(s)	310, 323, 419, 1143, 1202, 1307
HgaI	GACGC	2 cut(s)	1214, 1424
HhaI	GCGC	1 cut(s)	1432
Hin1II	CATG	7 cut(s)	493, 592, 661, 815, 1025, 1302, 1393
Hin6I	GCGC	1 cut(s)	1430
HinP1I	GCGC	1 cut(s)	1430
HindIII	AAGCTT	1 cut(s)	750
HinfI	GANTC	4 cut(s)	107, 297, 770, 1295
HpaII	CCGG	6 cut(s)	310, 323, 419, 1143, 1202, 1307
HphI	GGTGA	2 cut(s)	102, 730
Hpy166II	GTNNAC	1 cut(s)	1463
Hpy188I	TCNGA	4 cut(s)	268, 671, 705, 1317
Hpy188III	TCNNGA	4 cut(s)	170, 301, 1097, 1163
Hpy8I	GTNNAC	1 cut(s)	1463
Hpy99I	CGWCG	5 cut(s)	176, 185, 278, 453, 1418
HpyAV	CCTTC	8 cut(s)	207, 341, 715, 967, 1027, 1357, 1492, 1498
HpyCH4III	ACNGT	2 cut(s)	374, 1526
HpyCH4IV	ACGT	4 cut(s)	448, 578, 1441, 1626
HpyF10VI	GCNNNNNNNGC	5 cut(s)	10, 338, 644, 749, 984
HpyF3I	CTNAG	2 cut(s)	474, 962
HpySE526I	ACGT	4 cut(s)	448, 578, 1441, 1626
Hsp92II	CATG	7 cut(s)	493, 592, 661, 815, 1025, 1302, 1393
HspAI	GCGC	1 cut(s)	1430
KpnI	GGTACC	1 cut(s)	316
KroI	GCCGGC	2 cut(s)	418, 1142
KroNI	GCCGGC	2 cut(s)	420, 1144
Kzo9I	GATC	1 cut(s)	553
LmnI	GCTCC	5 cut(s)	18, 33, 172, 661, 1484
Lsp1109I	GCAGC	4 cut(s)	176, 344, 1249, 1331
LweI	GCATC	2 cut(s)	297, 475
MaeI	CTAG	2 cut(s)	846, 1544
MaeII	ACGT	4 cut(s)	448, 578, 1441, 1626
MaeIII	GTNAC	5 cut(s)	65, 108, 574, 929, 1000
MalI	GATC	1 cut(s)	555
MbiI	CCGCTC	1 cut(s)	664
MboI	GATC	1 cut(s)	553
MboII	GAAGA	8 cut(s)	130, 188, 1268, 1291, 1348, 1366, 1573, 1591
MfeI	CAATTG	1 cut(s)	208
MhlI	GDGCHC	1 cut(s)	1371
MlsI	TGGCCA	1 cut(s)	206
MluCI	AATT	5 cut(s)	208, 517, 558, 784, 1550
MluNI	TGGCCA	1 cut(s)	206
MlyI	GAGTC	1 cut(s)	116
MmeI	TCCRAC	3 cut(s)	324, 561, 816
Mox20I	TGGCCA	1 cut(s)	206
Mph1103I	ATGCAT	2 cut(s)	715, 1023
MroNI	GCCGGC	2 cut(s)	418, 1142
MscI	TGGCCA	1 cut(s)	206
MseI	TTAA	5 cut(s)	74, 765, 798, 1152, 1395
MslI	CAYNNNNRTG	2 cut(s)	54, 704
Msp20I	TGGCCA	1 cut(s)	206
MspA1I	CMGCKG	1 cut(s)	335
MspCI	CTTAAG	1 cut(s)	1151
MspI	CCGG	6 cut(s)	310, 323, 419, 1143, 1202, 1307
MspR9I	CCNGG	1 cut(s)	310
MunI	CAATTG	1 cut(s)	208
Mva1269I	GAATGC	3 cut(s)	715, 1000, 1246
MwoI	GCNNNNNNNGC	5 cut(s)	10, 338, 644, 749, 984
NaeI	GCCGGC	2 cut(s)	420, 1144
NciI	CCSGG	1 cut(s)	310
NcoI	CCATGG	1 cut(s)	489
NdeII	GATC	1 cut(s)	553
NgoMIV	GCCGGC	2 cut(s)	418, 1142
NlaIII	CATG	7 cut(s)	493, 592, 661, 815, 1025, 1302, 1393
NlaIV	GGNNCC	4 cut(s)	14, 314, 417, 1423
NmeAIII	GCCGAG	1 cut(s)	130
NmuCI	GTSAC	4 cut(s)	108, 574, 929, 1000
NsiI	ATGCAT	2 cut(s)	715, 1023
NspI	RCATGY	2 cut(s)	1025, 1393
PaeI	GCATGC	1 cut(s)	1025
PceI	AGGCCT	2 cut(s)	479, 920
PciI	ACATGT	1 cut(s)	1389
PcsI	WCGNNNNNNNCGW	3 cut(s)	168, 180, 186
PctI	GAATGC	3 cut(s)	715, 1000, 1246
PdiI	GCCGGC	2 cut(s)	420, 1144
PfeI	GAWTC	3 cut(s)	297, 770, 1295
PflFI	GACNNNGTC	1 cut(s)	188
PkrI	GCNGC	5 cut(s)	166, 334, 691, 1264, 1346
PleI	GAGTC	1 cut(s)	115
PpsI	GAGTC	1 cut(s)	115
Ppu21I	YACGTR	1 cut(s)	1442
PscI	ACATGT	1 cut(s)	1389
PspFI	CCCAGC	1 cut(s)	126
PspN4I	GGNNCC	4 cut(s)	14, 314, 417, 1423
PstI	CTGCAG	1 cut(s)	1349
PsyI	GACNNNGTC	1 cut(s)	188
PvuII	CAGCTG	1 cut(s)	335
RsaI	GTAC	4 cut(s)	314, 353, 1440, 1528
RsaNI	GTAC	4 cut(s)	313, 352, 1439, 1527
RseI	CAYNNNNRTG	2 cut(s)	54, 704
SaqAI	TTAA	5 cut(s)	74, 765, 798, 1152, 1395
SatI	GCNGC	5 cut(s)	165, 333, 690, 1263, 1345
Sau3AI	GATC	1 cut(s)	553
SchI	GAGTC	1 cut(s)	116
ScrFI	CCNGG	1 cut(s)	310
SduI	GDGCHC	1 cut(s)	1371
SfaNI	GCATC	2 cut(s)	297, 475
SfcI	CTRYAG	1 cut(s)	1345
SmiMI	CAYNNNNRTG	2 cut(s)	54, 704
SmlI	CTYRAG	3 cut(s)	23, 1151, 1268
SmoI	CTYRAG	3 cut(s)	23, 1151, 1268
SphI	GCATGC	1 cut(s)	1025
Sse9I	AATT	5 cut(s)	208, 517, 558, 784, 1550
SseBI	AGGCCT	2 cut(s)	479, 920
SsiI	CCGC	2 cut(s)	664, 689
SspMI	CTAG	2 cut(s)	846, 1544
StuI	AGGCCT	2 cut(s)	479, 920
StyD4I	CCNGG	1 cut(s)	308
StyI	CCWWGG	3 cut(s)	489, 1050, 1518
TaaI	ACNGT	2 cut(s)	374, 1526
TaiI	ACGT	4 cut(s)	451, 581, 1444, 1629
TaqI	TCGA	6 cut(s)	141, 216, 282, 451, 642, 1413
TasI	AATT	5 cut(s)	208, 517, 558, 784, 1550
TatI	WGTACW	1 cut(s)	1526
TauI	GCSGC	1 cut(s)	692
TfiI	GAWTC	3 cut(s)	297, 770, 1295
Tru1I	TTAA	5 cut(s)	74, 765, 798, 1152, 1395
Tru9I	TTAA	5 cut(s)	74, 765, 798, 1152, 1395
TscAI	CASTG	1 cut(s)	207
TseFI	GTSAC	4 cut(s)	108, 574, 929, 1000
TseI	GCWGC	4 cut(s)	164, 332, 1262, 1344
Tsp45I	GTSAC	4 cut(s)	108, 574, 929, 1000
TspDTI	ATGAA	7 cut(s)	424, 722, 969, 986, 1091, 1287, 1376
TspRI	CASTG	1 cut(s)	207
Tth111I	GACNNNGTC	1 cut(s)	188
Vha464I	CTTAAG	1 cut(s)	1151
XapI	RAATTY	2 cut(s)	517, 784
XceI	RCATGY	2 cut(s)	1025, 1393
XspI	CTAG	2 cut(s)	846, 1544
Zsp2I	ATGCAT	2 cut(s)	715, 1023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.