FvH4_4g09060

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005070
Physical Location & Seq
Reverse (-)
10208786 .. 10214863
6078 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09060.t1

Sequence Viewer

Length: 3279 bp
ATGATACTCATGTGTAATAAGGGAATTGCATGCTTGAGTGAGTCGGAGAACCCAATTGATCAGCACCAGCCTCCTCACTTGATGGAGAGCCCTTTCACTACCCCTCACTTTAGCTCTAATTCTACACATGCTTCCAGCCTTGTTTTGAATGAGGAGACTCCTCGCGTTAAGTTTCTGTGTAGTTTTTCCGGTAGTATATTGCCCCGGCCGCAGGATGGGAAGCTGCGGTATGTTGGTGGGGAGACACGAATTGTTTGTGTTCCGCGAGAGATTAAGTATGAGGAGTTGATGAGCAAGATGAGGGAGCTTTATGAAGGCGCAGCGGTGCTGAAGTACCAGCAGCCAGATGAGGACCTTGACGCTCTTGTTTCGGTTGTGAATGATGATGATGTGACCAACATGATGGAAGAGTATGATAAGTTGGGGTCAGGTGATGGTTTCACCAGGCTCAGGATTTTTTTGTTTCCGTATCCTGACCAAGATGGTGGTTCCTCGCACTATGATGGGGATGAAAGGGATCATGAGAGGAGATATGTTGATGCTCTCAATCACTTGAATGATGGTACAGAATTTAGGAAGCAGCATATGGAGTCCCCTGTGATTGGTTCAATTGATGATCTACATGGAGTGGAACAGTTCTTTAGTCCAATGAGTCTTGAAGGTGGTCTACATAGCCAGAGAAACGAGATACCTATGTCTCAGTATAACCTGCATCACCTCAAGATACCTCATACGGGGTCAGGGCAACACCATCAGCCCATTAGTCAAAGGTATAATGAAATGGAAGCTCCGTGGAGTCCTGCATACTATTCTCCTAGGCATCATGGATACCTCGATCCAAGACCAATGCCAGAGTTTCCATCTTCTCCTACCTCTGCACGGTACCGGATGCCATTCGCTGATGTACCTGATAGATGTTCAGACAGAACGCCTGAAGAATATGCACGGCCGCCATTGAATCATCAGGCTGGATATGAACAGCAACCACAATATTCTGACAATGTTGTATGGCTTCCAAGTGGAGCAATATCTGGTGAGAAGTCAGGTTTTCCAGGTAACATTTTTCATGGAAACAGTGTTCTTGAAGGAAACAGTGTATCTGAACACTGCCGAGTATGTTTCCAGAGGAACCAACCACGTTATGAGCACGCTAACTTGGTCAATGGATTTCATCAGGTTGCTAATACTTGTGCTGATTGCCGTCCAAAATTGCACCATGGTTTCACAACTGAGCAGAATAACGACTCTTCATCTCTATATAATGAGAATCAAAATCAATGGGTTGCACATCATTTGAATAGCCGGGCTGAGGAAGCAAGATCACACGTCTCTGGAACTGGAAAGTTGAATGATCACTACATTGTAGATGGTCCTGGCATGAGTTTGCCTCTTGTTCGTACTAATATGGTGGATATCCATCACCCGTCTACCAATTTAATTCATCAACGAGCTGGCTGTGAGATGGGAAATGAAGTGTTTCATGACCGACCTGTGGCTGGCCCACCCCACGTACACATTCCTCCGTCGGAAGACCGTGGTGTTCGATATGGAAATCCTCCATATGCCTACGGAGGAGATAATGTTTACCCAGTGTCTCATGGACATGCACAGGGACATGCAGTATGGAGAAATGTTCAGAGCACAATGCATGCTCTGCCCCCTTATGATGCATCCAATTCAGCTCCACAAATTAATGGTTCTGTTACTCCAGGATATCTCAAACACGAAGGTAGTCCAAGATTTTGCATTGCAGCAGACAATCCGAATCTTTGGGTTGAGTCATCGCAAAAGGTGTTAGGTTTTGATGGAAAATCTGTTCCTGACTATTCTTATGGCCAAGCTTTGAACTTGAACCCAAACACACTTGCTCATGAAATTCATCATCAATCTCCTCCAGAACTCATTCAACCTGCACCCGATATAGTAAGTTCTGCTACTCCTTTGAACGCCACCATTACAGTTATGTTGGAGTCAGAGTGTTTACCTAGAGAAGGACAGGAGGTGATTGATGGAGAGAAGATTGAGAACTCTGACATGCGGGTTATAAATCAACAAAAGTTGGATGATAAAAGTGAAGTGGCGCCTCTTGAGTCAGTAGATATAAATTCTCTCAAGCTCGCAGAAGAAGGTGGCGACAAAGATCATTCTACTCCTGAAGTTTCAAAGCTTGCAGTTAACAATTTGAGTTTCGTGCCTGAGTTGATTGCTAATATAAAAAAAGCAGGCATACATGGGGCAGAGGAGGTAAAAGCAAAAGTTGAGGAAACTACTGATCCTCAGAAGAGTATTTTGATTGCCAATGAAGCTGCAACAAACAGTTTGGAACCAGTGAACACTCCTGGAGACGGCGAAGTTGATTCTGATAGTGAGAATATGAATAATTCCAAAATTGAGCCAACAAAGGCCGAGGCAGAAGCTATTTCGAAGGGACTGCAGACAATAAAGAATGATGATCTAGAGGAGATCCGAGAATTAGGTTCTGGAACATATGGGGCTGTTTTTCATGGGAAGTGGAAGGGTTCCGATGTAGCAATAAAGAGAATAAAGGCCAGCTGTTTTGCTGGCCGACCTTCTGAAAGAGAGCGTTTGATTGCTGATTTCTGGAAGGAGGCTTTGATACTTAGTTCATTGCATCATCCAAATGTTGTTTCTTTCTATGGTATTGTGCGTGATGGTCCTGATGGTTCTTTAGCAACGGTGACTGAATTCATGGTCAATGGATCATTGAAACAGTTTTTGCAGAAAAAAGACAGGACAATTGATCGTCGTAAAAGATTAATTATTGCTATGGATGCTGCATTTGGGATGGAGTATCTGCATGGGAAAAATATAGTACATTTTGATTTGAAGTGTGAAAATCTGTTGGTAAATATGAGAGATCCCCAGCGGCCTGTATGCAAGATCGGTGATTTGGGGTTGTCAAAAGTGAAACAACAGACGTTAGTGTCTGGAGGTGTTCGTGGAACTTTGCCCTGGATGGCACCTGAGCTTCTGAGTGGGAAAAGTCATATGGTGACAGAGAAGATTGATGTTTACTCGTTTGGGATTGTTATGTGGGAACTGCTTACTGGCGATGAACCCTATACAGATATGCATTGTGCTTCCATAATTGGAGGAATTGTGAACAACACTTTGCGTCCTCAAATTCCAACATGGTGTGATCCTGAATGGAAGTCTTTGATGGAAAGTTGTTGGGGCTCCGAGCCTGCTCAGAGGCCATCATTTTCCGAGATCTCTCAGAAGCTGAGGAATATGGCTGCTGCAATGAATGTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1093

Amino Acids

121.56

Weight (kDa)

5.55

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 71 - 156 1.5e-17 PB1 domain
Pkinase PF00069 820 - 1080 4.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 821 - 1082 4e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2045
AasI GACNNNNNNGTC 1 cut(s) 2944
Acc36I ACCTGC 2 cut(s) 717, 1918
Acc65I GGTACC 1 cut(s) 882
AccB1I GGYRCC 3 cut(s) 882, 2080, 2980
AccI GTMKAC 2 cut(s) 667, 1427
AccII CGCG 2 cut(s) 165, 265
AciI CCGC 7 cut(s) 209, 226, 263, 323, 950, 2038, 2887
AclWI GGATC 7 cut(s) 525, 830, 2267, 2458, 2728, 2873, 3155
AcoI YGGCCR 4 cut(s) 206, 947, 1834, 2563
AcsI RAATTY 5 cut(s) 569, 1875, 2104, 2705, 3144
AcuI CTGAAG 3 cut(s) 350, 954, 2175
AcyI GRCGYC 1 cut(s) 2081
AfaI GTAC 7 cut(s) 335, 565, 884, 906, 1399, 1512, 2835
AflIII ACRYGT 1 cut(s) 1324
AjiI CACGTC 1 cut(s) 1327
AjnI CCWGG 6 cut(s) 443, 1051, 1372, 1708, 2338, 2972
AjuI GAANNNNNNNTTGG 2 cut(s) 2632, 2664
AloI GAACNNNNNNTCC 4 cut(s) 1062, 1094, 1800, 1832
Alw21I GWGCWC 2 cut(s) 1149, 1643
Alw26I GTCTC 6 cut(s) 149, 236, 702, 1333, 1599, 2337
AlwI GGATC 7 cut(s) 525, 830, 2267, 2458, 2728, 2873, 3155
AlwNI CAGNNNCTG 1 cut(s) 3244
AoxI GGCC 9 cut(s) 206, 947, 1498, 1834, 2403, 2547, 2563, 2888, 3215
ApeKI GCWGC 9 cut(s) 223, 320, 340, 580, 1751, 2306, 2795, 3257, 3260
ApoI RAATTY 5 cut(s) 569, 1875, 2104, 2705, 3144
AseI ATTAAT 2 cut(s) 1692, 2777
Asp700I GAANNNNTTC 2 cut(s) 1476, 1902
Asp718I GGTACC 1 cut(s) 882
AspA2I CCTAGG 1 cut(s) 815
AspLEI GCGC 2 cut(s) 320, 2083
AspS9I GGNCC 4 cut(s) 352, 1370, 1499, 2675
AsuC2I CCSGG 2 cut(s) 205, 1304
AsuHPI GGTGA 9 cut(s) 433, 443, 707, 1046, 1412, 2014, 2710, 2918, 3025
AsuII TTCGAA 1 cut(s) 2423
AvaII GGWCC 3 cut(s) 352, 1370, 2675
AvrII CCTAGG 1 cut(s) 815
BaeI ACNNNNGTAYC 3 cut(s) 29, 680, 713
BalI TGGCCA 1 cut(s) 1836
BanI GGYRCC 3 cut(s) 882, 2080, 2980
BanII GRGCYC 2 cut(s) 92, 3200
BarI GAAGNNNNNNTAC 2 cut(s) 651, 683
BbsI GAAGAC 1 cut(s) 1536
Bbv12I GWGCWC 2 cut(s) 1149, 1643
BbvCI CCTCAGC 2 cut(s) 1308, 3245
BbvI GCAGC 9 cut(s) 210, 332, 352, 592, 1763, 2293, 2782, 3244, 3247
BceAI ACGGC 3 cut(s) 962, 1185, 2362
BcgI CGANNNNNNTGC 2 cut(s) 2413, 2447
BciT130I CCWGG 6 cut(s) 445, 1053, 1374, 1710, 2340, 2974
BciVI GTATCC 2 cut(s) 480, 821
BclI TGATCA 2 cut(s) 58, 1351
BcnI CCSGG 2 cut(s) 205, 1304
BcoDI GTCTC 6 cut(s) 149, 236, 702, 1333, 1599, 2337
BfaI CTAG 3 cut(s) 816, 1986, 2456
BfmI CTRYAG 1 cut(s) 2432
BfoI RGCGCY 1 cut(s) 2084
BfuAI ACCTGC 2 cut(s) 717, 1918
BfuI GTATCC 2 cut(s) 480, 821
BglII AGATCT 1 cut(s) 3231
BlnI CCTAGG 1 cut(s) 815
Bme1390I CCNGG 8 cut(s) 205, 445, 1053, 1304, 1374, 1710, 2340, 2974
Bme18I GGWCC 3 cut(s) 352, 1370, 2675
BmgBI CACGTC 1 cut(s) 1327
BmgT120I GGNCC 4 cut(s) 352, 1370, 1499, 2675
BmiI GGNNCC 8 cut(s) 490, 884, 1130, 2082, 2325, 2521, 2982, 3199
BmrFI CCNGG 8 cut(s) 205, 445, 1053, 1304, 1374, 1710, 2340, 2974
BmrI ACTGGG 1 cut(s) 1583
BmsI GCATC 8 cut(s) 529, 721, 829, 879, 1657, 1679, 2641, 2782
BmuI ACTGGG 1 cut(s) 1583
BpiI GAAGAC 1 cut(s) 1536
BplI GAGNNNNNCTC 2 cut(s) 1372, 1404
BpmI CTGGAG 4 cut(s) 1692, 1878, 2361, 2970
Bpu10I CCTNAGC 4 cut(s) 449, 1308, 2985, 3245
Bpu14I TTCGAA 1 cut(s) 2423
BpuEI CTTGAG 4 cut(s) 55, 704, 2096, 2108
BpuMI CCSGG 2 cut(s) 205, 1304
BsaAI YACGTR 1 cut(s) 1510
BsaBI GATNNNNATC 1 cut(s) 1416
BsaHI GRCGYC 1 cut(s) 2081
BsaJI CCNNGG 7 cut(s) 203, 791, 815, 1216, 1534, 2406, 2972
BsaWI WCCGGW 2 cut(s) 188, 885
BsaXI ACNNNNNCTCC 6 cut(s) 1566, 1596, 1961, 1991, 3105, 3135
Bse1I ACTGG 4 cut(s) 1342, 1589, 2327, 3073
Bse3DI GCAATG 3 cut(s) 1746, 2627, 3270
Bse8I GATNNNNATC 1 cut(s) 1416
BseBI CCWGG 6 cut(s) 445, 1053, 1374, 1710, 2340, 2974
BseDI CCNNGG 7 cut(s) 203, 791, 815, 1216, 1534, 2406, 2972
BseGI GGATG 9 cut(s) 220, 514, 894, 1670, 2068, 2635, 2797, 2811, 2982
BseJI GATNNNNATC 1 cut(s) 1416
BseMI GCAATG 3 cut(s) 1746, 2627, 3270
BseNI ACTGG 4 cut(s) 1342, 1589, 2327, 3073
BseRI GAGGAG 9 cut(s) 63, 150, 167, 296, 541, 1587, 1881, 2255, 2474
BseX3I CGGCCG 2 cut(s) 206, 947
BseXI GCAGC 9 cut(s) 210, 332, 352, 592, 1763, 2293, 2782, 3244, 3247
BseYI CCCAGC 1 cut(s) 2883
BsgI GTGCAG 2 cut(s) 861, 1896
Bsh1236I CGCG 2 cut(s) 165, 265
Bsh1285I CGRYCG 2 cut(s) 209, 950
BshFI GGCC 9 cut(s) 208, 949, 1500, 1836, 2405, 2549, 2565, 2890, 3217
BshNI GGYRCC 3 cut(s) 882, 2080, 2980
BsiEI CGRYCG 2 cut(s) 209, 950
BsiHKAI GWGCWC 2 cut(s) 1149, 1643
BsiSI CCGG 4 cut(s) 189, 205, 886, 1303
BslFI GGGAC 3 cut(s) 577, 1626, 2442
BsmAI GTCTC 6 cut(s) 149, 236, 702, 1333, 1599, 2337
BsmBI CGTCTC 2 cut(s) 1333, 2337
BsmFI GGGAC 3 cut(s) 577, 1626, 2442
BsnI GGCC 9 cut(s) 208, 949, 1500, 1836, 2405, 2549, 2565, 2890, 3217
Bsp119I TTCGAA 1 cut(s) 2423
Bsp1286I GDGCHC 4 cut(s) 92, 1149, 1643, 3200
Bsp19I CCATGG 1 cut(s) 1216
BspACI CCGC 7 cut(s) 209, 226, 263, 323, 950, 2038, 2887
BspANI GGCC 9 cut(s) 208, 949, 1500, 1836, 2405, 2549, 2565, 2890, 3217
BspFNI CGCG 2 cut(s) 165, 265
BspHI TCATGA 3 cut(s) 520, 1480, 1870
BspLI GGNNCC 8 cut(s) 490, 884, 1130, 2082, 2325, 2521, 2982, 3199
BspMAI CTGCAG 1 cut(s) 2436
BspMI ACCTGC 2 cut(s) 717, 1918
BspPI GGATC 7 cut(s) 525, 830, 2267, 2458, 2728, 2873, 3155
BspT104I TTCGAA 1 cut(s) 2423
BspT107I GGYRCC 3 cut(s) 882, 2080, 2980
BsrDI GCAATG 3 cut(s) 1746, 2627, 3270
BsrI ACTGG 4 cut(s) 1342, 1589, 2327, 3073
BssECI CCNNGG 7 cut(s) 203, 791, 815, 1216, 1534, 2406, 2972
BssNI GRCGYC 1 cut(s) 2081
BssT1I CCWWGG 2 cut(s) 815, 1216
Bst2UI CCWGG 6 cut(s) 445, 1053, 1374, 1710, 2340, 2974
Bst4CI ACNGT 9 cut(s) 636, 882, 1076, 1094, 1535, 1960, 2318, 2698, 2733
Bst6I CTCTTC 3 cut(s) 402, 1252, 2276
BstACI GRCGYC 1 cut(s) 2081
BstAPI GCANNNNNTGC 1 cut(s) 1654
BstBAI YACGTR 1 cut(s) 1510
BstBI TTCGAA 1 cut(s) 2423
BstDSI CCRYGG 3 cut(s) 791, 1216, 1534
BstENI CCTNNNNNAGG 1 cut(s) 1989
BstF5I GGATG 9 cut(s) 220, 514, 894, 1670, 2068, 2635, 2797, 2811, 2982
BstFNI CGCG 2 cut(s) 165, 265
BstH2I RGCGCY 1 cut(s) 2084
BstHHI GCGC 2 cut(s) 320, 2083
BstMAI GTCTC 6 cut(s) 149, 236, 702, 1333, 1599, 2337
BstMCI CGRYCG 2 cut(s) 209, 950
BstMWI GCNNNNNNNGC 5 cut(s) 208, 1313, 1654, 2303, 2792
BstNI CCWGG 6 cut(s) 445, 1053, 1374, 1710, 2340, 2974
BstNSI RCATGY 6 cut(s) 33, 131, 1607, 1619, 1652, 2038
BstSCI CCNGG 8 cut(s) 203, 443, 1051, 1302, 1372, 1708, 2338, 2972
BstSFI CTRYAG 1 cut(s) 2432
BstUI CGCG 2 cut(s) 165, 265
BstV1I GCAGC 9 cut(s) 210, 332, 352, 592, 1763, 2293, 2782, 3244, 3247
BstV2I GAAGAC 1 cut(s) 1536
BstX2I RGATCY 3 cut(s) 2463, 2878, 3231
BstXI CCANNNNNNTGG 2 cut(s) 403, 485
BstYI RGATCY 3 cut(s) 2463, 2878, 3231
BstZI CGGCCG 2 cut(s) 206, 947
BsuI GTATCC 2 cut(s) 480, 821
BsuRI GGCC 9 cut(s) 208, 949, 1500, 1836, 2405, 2549, 2565, 2890, 3217
BtgI CCRYGG 3 cut(s) 791, 1216, 1534
BtgZI GCGATG 2 cut(s) 1767, 3087
BtrI CACGTC 1 cut(s) 1327
BtsCI GGATG 9 cut(s) 220, 514, 894, 1670, 2068, 2635, 2797, 2811, 2982
BtsI GCAGTG 1 cut(s) 1105
BtsIMutI CAGTG 5 cut(s) 1081, 1099, 1105, 1596, 2334
BveI ACCTGC 2 cut(s) 717, 1918
CaiI CAGNNNCTG 1 cut(s) 3244
CciI TCATGA 3 cut(s) 520, 1480, 1870
CfoI GCGC 2 cut(s) 320, 2083
Cfr13I GGNCC 4 cut(s) 352, 1370, 1499, 2675
CseI GACGC 2 cut(s) 368, 3125
Csp6I GTAC 7 cut(s) 334, 564, 883, 905, 1398, 1511, 2834
CviQI GTAC 7 cut(s) 334, 564, 883, 905, 1398, 1511, 2834
DinI GGCGCC 1 cut(s) 2082
DrdI GACNNNNNNGTC 1 cut(s) 2944
DseDI GACNNNNNNGTC 1 cut(s) 2944
EaeI YGGCCR 4 cut(s) 206, 947, 1834, 2563
EagI CGGCCG 2 cut(s) 206, 947
Eam1104I CTCTTC 3 cut(s) 402, 1252, 2276
EarI CTCTTC 3 cut(s) 402, 1252, 2276
EclXI CGGCCG 2 cut(s) 206, 947
Eco130I CCWWGG 2 cut(s) 815, 1216
Eco24I GRGCYC 2 cut(s) 92, 3200
Eco32I GATATC 2 cut(s) 1414, 1715
Eco47I GGWCC 3 cut(s) 352, 1370, 2675
Eco52I CGGCCG 2 cut(s) 206, 947
Eco57I CTGAAG 3 cut(s) 350, 954, 2175
EcoNI CCTNNNNNAGG 1 cut(s) 1989
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 2705
EcoRII CCWGG 6 cut(s) 443, 1051, 1372, 1708, 2338, 2972
EcoRV GATATC 2 cut(s) 1414, 1715
EcoT14I CCWWGG 2 cut(s) 815, 1216
EcoT22I ATGCAT 3 cut(s) 1650, 1672, 3096
EcoT38I GRGCYC 2 cut(s) 92, 3200
EgeI GGCGCC 1 cut(s) 2082
EheI GGCGCC 1 cut(s) 2082
ErhI CCWWGG 2 cut(s) 815, 1216
Esp3I CGTCTC 2 cut(s) 1333, 2337
FaqI GGGAC 3 cut(s) 577, 1626, 2442
FauI CCCGC 1 cut(s) 2031
FauNDI CATATG 4 cut(s) 585, 1561, 2488, 3009
FbaI TGATCA 2 cut(s) 58, 1351
FblI GTMKAC 2 cut(s) 667, 1427
FokI GGATG 9 cut(s) 227, 521, 901, 1657, 2075, 2622, 2804, 2818, 2989
FriOI GRGCYC 2 cut(s) 92, 3200
FspBI CTAG 3 cut(s) 816, 1986, 2456
GlaI GCGC 2 cut(s) 319, 2082
GsaI CCCAGC 1 cut(s) 2887
GsuI CTGGAG 4 cut(s) 1692, 1878, 2361, 2970
HaeII RGCGCY 1 cut(s) 2084
HaeIII GGCC 9 cut(s) 208, 949, 1500, 1836, 2405, 2549, 2565, 2890, 3217
HapII CCGG 4 cut(s) 189, 205, 886, 1303
HgaI GACGC 2 cut(s) 368, 3125
HhaI GCGC 2 cut(s) 320, 2083
Hin1I GRCGYC 1 cut(s) 2081
Hin6I GCGC 2 cut(s) 318, 2081
HinP1I GCGC 2 cut(s) 318, 2081
HincII GTYRAC 1 cut(s) 2176
HindII GTYRAC 1 cut(s) 2176
HindIII AAGCTT 2 cut(s) 1839, 2165
HpaI GTTAAC 1 cut(s) 2176
HpaII CCGG 4 cut(s) 189, 205, 886, 1303
HphI GGTGA 9 cut(s) 433, 443, 707, 1046, 1412, 2014, 2710, 2918, 3025
Hpy166II GTNNAC 9 cut(s) 668, 1428, 1513, 1585, 1982, 2176, 2332, 3034, 3124
Hpy8I GTNNAC 9 cut(s) 668, 1428, 1513, 1585, 1982, 2176, 2332, 3034, 3124
Hpy99I CGWCG 2 cut(s) 1528, 2769
HpyCH4III ACNGT 9 cut(s) 636, 882, 1076, 1094, 1535, 1960, 2318, 2698, 2733
HpyCH4IV ACGT 4 cut(s) 1138, 1326, 1509, 2939
HpyF10VI GCNNNNNNNGC 5 cut(s) 208, 1313, 1654, 2303, 2792
HpySE526I ACGT 4 cut(s) 1138, 1326, 1509, 2939
Hsp92I GRCGYC 1 cut(s) 2081
HspAI GCGC 2 cut(s) 318, 2081
KasI GGCGCC 1 cut(s) 2080
KpnI GGTACC 1 cut(s) 886
Ksp22I TGATCA 2 cut(s) 58, 1351
KspAI GTTAAC 1 cut(s) 2176
LmnI GCTCC 5 cut(s) 304, 793, 1022, 1687, 3203
Lsp1109I GCAGC 9 cut(s) 210, 332, 352, 592, 1763, 2293, 2782, 3244, 3247
LweI GCATC 8 cut(s) 529, 721, 829, 879, 1657, 1679, 2641, 2782
MaeI CTAG 3 cut(s) 816, 1986, 2456
MaeII ACGT 4 cut(s) 1138, 1326, 1509, 2939
MaeIII GTNAC 5 cut(s) 391, 1055, 1702, 2698, 3013
MboII GAAGA 9 cut(s) 419, 855, 947, 1239, 1541, 2029, 2135, 2293, 3034
MfeI CAATTG 3 cut(s) 54, 609, 2757
MflI RGATCY 3 cut(s) 2463, 2878, 3231
MhlI GDGCHC 4 cut(s) 92, 1149, 1643, 3200
MlsI TGGCCA 1 cut(s) 1836
MluNI TGGCCA 1 cut(s) 1836
Mly113I GGCGCC 1 cut(s) 2081
MlyI GAGTC 9 cut(s) 50, 151, 599, 661, 805, 1238, 1787, 1979, 2099
MmeI TCCRAC 5 cut(s) 24, 1506, 1947, 2040, 3173
Mox20I TGGCCA 1 cut(s) 1836
Mph1103I ATGCAT 3 cut(s) 1650, 1672, 3096
MroXI GAANNNNTTC 2 cut(s) 1476, 1902
MscI TGGCCA 1 cut(s) 1836
MseI TTAA 7 cut(s) 168, 273, 1436, 1692, 2175, 2777, 3273
MslI CAYNNNNRTG 4 cut(s) 501, 555, 1602, 2640
Msp20I TGGCCA 1 cut(s) 1836
MspA1I CMGCKG 3 cut(s) 323, 2553, 2887
MspI CCGG 4 cut(s) 189, 205, 886, 1303
MspR9I CCNGG 8 cut(s) 205, 445, 1053, 1304, 1374, 1710, 2340, 2974
MunI CAATTG 3 cut(s) 54, 609, 2757
MvaI CCWGG 6 cut(s) 445, 1053, 1374, 1710, 2340, 2974
MvnI CGCG 2 cut(s) 165, 265
MwoI GCNNNNNNNGC 5 cut(s) 208, 1313, 1654, 2303, 2792
NarI GGCGCC 1 cut(s) 2081
NciI CCSGG 2 cut(s) 205, 1304
NcoI CCATGG 1 cut(s) 1216
NdeI CATATG 4 cut(s) 585, 1561, 2488, 3009
NlaIV GGNNCC 8 cut(s) 490, 884, 1130, 2082, 2325, 2521, 2982, 3199
NmeAIII GCCGAG 2 cut(s) 1136, 2431
NmuCI GTSAC 3 cut(s) 391, 2698, 3013
NsiI ATGCAT 3 cut(s) 1650, 1672, 3096
NspI RCATGY 6 cut(s) 33, 131, 1607, 1619, 1652, 2038
NspV TTCGAA 1 cut(s) 2423
PaeI GCATGC 2 cut(s) 33, 1652
PagI TCATGA 3 cut(s) 520, 1480, 1870
PdmI GAANNNNTTC 2 cut(s) 1476, 1902
PfeI GAWTC 4 cut(s) 958, 1267, 1765, 2357
PfoI TCCNGGA 2 cut(s) 1708, 2338
PleI GAGTC 9 cut(s) 49, 151, 598, 660, 804, 1238, 1786, 1978, 2098
PluTI GGCGCC 1 cut(s) 2084
PpsI GAGTC 9 cut(s) 49, 151, 598, 660, 804, 1238, 1786, 1978, 2098
Ppu21I YACGTR 1 cut(s) 1510
PpuMI RGGWCCY 1 cut(s) 352
PshBI ATTAAT 2 cut(s) 1692, 2777
PsiI TTATAA 1 cut(s) 2045
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 6 cut(s) 443, 1051, 1372, 1708, 2338, 2972
PspFI CCCAGC 1 cut(s) 2883
PspGI CCWGG 6 cut(s) 443, 1051, 1372, 1708, 2338, 2972
PspN4I GGNNCC 8 cut(s) 490, 884, 1130, 2082, 2325, 2521, 2982, 3199
PspPI GGNCC 4 cut(s) 352, 1370, 1499, 2675
PspPPI RGGWCCY 1 cut(s) 352
PstI CTGCAG 1 cut(s) 2436
PstNI CAGNNNCTG 1 cut(s) 3244
PsuI RGATCY 3 cut(s) 2463, 2878, 3231
PvuII CAGCTG 1 cut(s) 2553
RsaI GTAC 7 cut(s) 335, 565, 884, 906, 1399, 1512, 2835
RsaNI GTAC 7 cut(s) 334, 564, 883, 905, 1398, 1511, 2834
RseI CAYNNNNRTG 4 cut(s) 501, 555, 1602, 2640
SaqAI TTAA 7 cut(s) 168, 273, 1436, 1692, 2175, 2777, 3273
Sau96I GGNCC 4 cut(s) 352, 1370, 1499, 2675
SchI GAGTC 9 cut(s) 50, 151, 599, 661, 805, 1238, 1787, 1979, 2099
ScrFI CCNGG 8 cut(s) 205, 445, 1053, 1304, 1374, 1710, 2340, 2974
SduI GDGCHC 4 cut(s) 92, 1149, 1643, 3200
SfaNI GCATC 8 cut(s) 529, 721, 829, 879, 1657, 1679, 2641, 2782
SfcI CTRYAG 1 cut(s) 2432
SfoI GGCGCC 1 cut(s) 2082
SfuI TTCGAA 1 cut(s) 2423
SinI GGWCC 3 cut(s) 352, 1370, 2675
SmiMI CAYNNNNRTG 4 cut(s) 501, 555, 1602, 2640
SmlI CTYRAG 4 cut(s) 34, 719, 2087, 2111
SmoI CTYRAG 4 cut(s) 34, 719, 2087, 2111
SphI GCATGC 2 cut(s) 33, 1652
SsiI CCGC 7 cut(s) 209, 226, 263, 323, 950, 2038, 2887
SspDI GGCGCC 1 cut(s) 2080
SspI AATATT 1 cut(s) 992
SspMI CTAG 3 cut(s) 816, 1986, 2456
StyD4I CCNGG 8 cut(s) 203, 443, 1051, 1302, 1372, 1708, 2338, 2972
StyI CCWWGG 2 cut(s) 815, 1216
TaaI ACNGT 9 cut(s) 636, 882, 1076, 1094, 1535, 1960, 2318, 2698, 2733
TaiI ACGT 4 cut(s) 1141, 1329, 1512, 2942
TaqI TCGA 3 cut(s) 834, 1543, 2423
TaqII GACCGA 1 cut(s) 1500
TatI WGTACW 1 cut(s) 2833
TauI GCSGC 3 cut(s) 211, 952, 2890
TfiI GAWTC 4 cut(s) 958, 1267, 1765, 2357
Tru1I TTAA 7 cut(s) 168, 273, 1436, 1692, 2175, 2777, 3273
Tru9I TTAA 7 cut(s) 168, 273, 1436, 1692, 2175, 2777, 3273
TscAI CASTG 5 cut(s) 1081, 1099, 1112, 1596, 2334
TseFI GTSAC 3 cut(s) 391, 2698, 3013
TseI GCWGC 9 cut(s) 223, 320, 340, 580, 1751, 2306, 2795, 3257, 3260
Tsp45I GTSAC 3 cut(s) 391, 2698, 3013
TspGWI ACGGA 4 cut(s) 456, 780, 1512, 1584
TspRI CASTG 5 cut(s) 1081, 1099, 1112, 1596, 2334
VpaK11BI GGWCC 3 cut(s) 352, 1370, 2675
VspI ATTAAT 2 cut(s) 1692, 2777
XagI CCTNNNNNAGG 1 cut(s) 1989
XapI RAATTY 5 cut(s) 569, 1875, 2104, 2705, 3144
XbaI TCTAGA 1 cut(s) 2455
XceI RCATGY 6 cut(s) 33, 131, 1607, 1619, 1652, 2038
XmaJI CCTAGG 1 cut(s) 815
XmiI GTMKAC 2 cut(s) 667, 1427
XmnI GAANNNNTTC 2 cut(s) 1476, 1902
XspI CTAG 3 cut(s) 816, 1986, 2456
Zsp2I ATGCAT 3 cut(s) 1650, 1672, 3096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.