Rh4AG118400

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005070
Physical Location & Seq
Forward (+)
25560269 .. 25566496
6228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG118400.1

Sequence Viewer

Length: 3012 bp
ATGTGTAATAAAGGAATTGCATGCTTGAGTGAGTCGGATAACCCAATTGATCAGCAGCAGCAACAGGCTGTCTACTTGATGGAGAGCCCTTCCACTACCCCTCACTTTGGCTCTAATTCCACCCCTGTTTCCAGCCCTGTTTTGAATGAGGACAATCCCCCTCGTGTTAAATTTCTGTGTAGTTTCTCCGGTAGTATATTGCCTCGGCCGCAGGATGGGAGGCTCCGATATGTAGGTGGGGAGACACGGATTGTTAGTGTTCCGCGAGACATTCGGTATGAGGAGTTGATGAGCAAGATGAGGGAGCTTTATGAAGGGGCGGCGGTGCTTAAGTACCAGCAACCGGATGAGGACCTTGACGCCCTTGTCTCGGTTGTGAATGATGATGATGTGACCAACATGATGGAAGAGTATGATAAGTTGGGGTCGGGTGATGGTTTCACCAGGCTCAGGATTTTTTTGTTTTCGTATCCCGACCAAGATGGTGGCTCCTCACACTATGACGGGGATGAAAGGGATCATGAGAGGAGGTATGTTGATGCGCTCAATAACTTAAATGATGGTTCCGAATTTAGGAAGCAGCATTTAGAGTCCCCTATTATCACTTCAGTTGATGATCTCCATGGAGTGGAACAGTTCTTTAGTCCAATGAGTCTCGAGGGTGGCCTCCATAGCCAGAGAAACGAGATATCCATGTCTCATTATAACCTGCATCGCCTCAAAATACCTCATATAGGTTCAGGGCAACACCATCAGCCCATTAGCCAAAGGTATAATGACATGGAAGCTCCGTGGAGTCCTGCATACTATTCTCCTAGGCATCATGGGCACATTGATCCAAGACCAATGGCAGAGTTTCCAACTTCTCCTACCTCTGCACGGTACCGTATGCCATTTGCAGATGTACCTGATAAATGTTCAGATAGAATGCCTGAAGAATATGCACGGCCGCCATTGAATCATCAGCCTGGATATGAACACCAGCCACAGTATTCTGATAATGCTGTATGGCTTCCAAGTGGAGCTATATCTGGTGAGAAGTCAGGCTTTCCAGGTAACATTTTTCATGGAAACAATGTTCTCGAAGGAAACAGTGTCTCTGAACACTGTCGGGTATGTTTCCAAAGAAACCAACCACATTTCGAGCAACCCAACATGGTCAATGGATTTCATCAGGTTGCTAATACATGTGCTGATTGCCGTCCAAAGTTGCACCATGGTTACACAACTGAGCAGAATAACGATTCTTCACCTCTATATAATGAGAATCAAAATCAATGGGCTCCACACCGTTTGAATAGCCGGGCTGAGGAAGCAAGATCACATGTCTCTGGAACTGGAAAATTGAACGATCACTACATTGTAGATGGTCCTGGCATGAATTTGCCTCTTGTTCATAATAATATGGTGGATGTCCATCACGTCTCTACCAATTTTGTCCATCAACGAGCTGGGTCTGAAATGGGAAATGACGTGTTTCATGACCGACCTGTGGCTGGCCCACCCCACGTACACATTTCTCCTGTAGAAGACCGTGGCGTTCCATATGGAAATCTTCCTTATGCTTACGGAGGAGATAATGTTTACCCAGTGTCTCATGGACATGCACCGGGACATGGAGTATGGAGAAATGTTCATAGCACAATGCATCCTGTGCCCCCTTACGATGCATCCAATTCAGCTCCCCAAATTAATGGTTCTGTCAATCCAGGATATCTCAGACACGAGGGTAGTCCAAGATTTTGCATTGCAGCAGACAATCAGAATCTTTGGGTTGAATCATCGCAGAAGGTGTTAGGTTTTGACGGAAGATCTGTTCCAGACTATTCTTATGGCCAAGCTTTGAACTTGAACCCTAACACACTTGCTCATGAAAATCATCATCAATTTCCTCCAGAACTCATTCAACCCACACCTGATATAGTAAGTTCTGCTACCCCTTTGGACGGCGTCACTAGAGTTATGTTGGATACAGAGAGTTTACCTAGAGAAGGAAGGGAGGTGATTGATGGAGAGAAGGTTGAGAACTCTGATATGAAGGTTATAAATAAAAAAGAATTGGGTGATATAAGTGAAGTGGTGCCTCTCGAGTTAGTAAATTCAAATTGTCTCAAACTCGCAGAAGAAAGTGGTGACAAGGATCATTCTACTCCTGAAATTTCAAAGCTTTCAGCTAACAATTTGAGTTTTGTGCCTGAGTTGATTGCTTCTGTAAAAAAAGTGGCCATATATGGGGTAGAGGAGGTAAAAGCAAAAGTTGAGGAAAGTACTGATTCTCAGAAGAGTAATCCGATTGCCAATGAAGCTGCAACAAACAGTTTGGAACCAGTGAACACTCCTGGAGATGGCGAAGTGGATTCTGATAGTGAGAATCTGAATAATTCCAAAATTGAGCCAACAAAGGCCGAGGCAGAAGCTATTTCCAAGGGATTGCAGACAATAAAGAATGATGATCTTGAGGAGATCCGAGAATTAGGTTCTGGAACATATGGAGCTGTTTTTCATGGGAAGTGGAAGGGTTCAGATGTAGCAATAAAGAGAATAAAAGCCAGCTGTTTTGCTGGCCGACCTTCTGAAAGAGAACGTTTGATTGCTGATTTCTGGAAGGAGGCTTTGATACTTAGTTCATTGCATCATCCAAATGTTGTTTCTTTCTATGGTATTGTGCGTGATGGTCCTGATGGCTCTTTAGCAACTGTTACTGAATTCATGGTTAATGGATCTTTGAAACAGTTTTTGCAGAAAAAAGACAGAACAATTGATCGTCGTAAACGATTGATTATAGCTATGGATGCTGCATTCGGGATGGAGTATCTGCATGGAAAAAATATAGTACATTTTGATTTGAAGTGTGAAAATCTGTTGGTAAATATGAGAGATCCACAGCGGCCTGTATGCAAGGTTGGCTGGTTTAATTACCTTCTTTTCACTTGTAGTGTCTTTTCACCTACAGTTTTTCTAACCATGTATGTGTTGGGACTCGCATCATATGTGCATTCTTTAGATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1003

Amino Acids

111.95

Weight (kDa)

5.52

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 72 - 155 1.1e-17 PB1 domain
Pkinase PF00069 820 - 958 2.4e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 821 - 969 5.3e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 705, 2045
AasI GACNNNNNNGTC 1 cut(s) 365
Acc36I ACCTGC 1 cut(s) 717
Acc65I GGTACC 1 cut(s) 882
AccB1I GGYRCC 2 cut(s) 882, 2080
AccB7I CCANNNNNTGG 1 cut(s) 628
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 265
AciI CCGC 6 cut(s) 209, 263, 320, 323, 950, 2887
AclI AACGTT 1 cut(s) 2584
AclWI GGATC 6 cut(s) 525, 830, 2148, 2458, 2728, 2873
AcoI YGGCCR 5 cut(s) 206, 947, 1834, 2223, 2563
AcsI RAATTY 6 cut(s) 170, 569, 1381, 2098, 2157, 2705
AcuI CTGAAG 2 cut(s) 591, 954
AcyI GRCGYC 2 cut(s) 360, 1950
AfaI GTAC 6 cut(s) 335, 884, 906, 1512, 2269, 2835
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 3 cut(s) 1187, 1324, 1473
AjiI CACGTC 2 cut(s) 1423, 1474
AjnI CCWGG 6 cut(s) 443, 967, 1051, 1372, 1708, 2338
AjuI GAANNNNNNNTTGG 2 cut(s) 2632, 2664
AloI GAACNNNNNNTCC 4 cut(s) 1062, 1094, 1800, 1832
AlwI GGATC 6 cut(s) 525, 830, 2148, 2458, 2728, 2873
Ama87I CYCGRG 2 cut(s) 656, 2087
AoxI GGCC 9 cut(s) 206, 664, 947, 1498, 1834, 2223, 2403, 2563, 2888
ApeKI GCWGC 6 cut(s) 55, 58, 580, 1751, 2306, 2795
ApoI RAATTY 6 cut(s) 170, 569, 1381, 2098, 2157, 2705
AseI ATTAAT 1 cut(s) 1692
Asp700I GAANNNNTTC 1 cut(s) 1902
Asp718I GGTACC 1 cut(s) 882
AspA2I CCTAGG 1 cut(s) 815
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 4 cut(s) 352, 1370, 1499, 2675
AsuC2I CCSGG 2 cut(s) 1304, 1611
AsuHPI GGTGA 8 cut(s) 433, 443, 1046, 1242, 2014, 2075, 2144, 2937
AvaI CYCGRG 2 cut(s) 656, 2087
AvaII GGWCC 3 cut(s) 352, 1370, 2675
AvrII CCTAGG 1 cut(s) 815
BaeGI GKGCMC 2 cut(s) 831, 1659
BalI TGGCCA 2 cut(s) 1836, 2225
BanI GGYRCC 2 cut(s) 882, 2080
BanII GRGCYC 2 cut(s) 89, 1285
BauI CACGAG 2 cut(s) 162, 1724
BbsI GAAGAC 1 cut(s) 1536
BbvCI CCTCAGC 1 cut(s) 1308
BbvI GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
BceAI ACGGC 3 cut(s) 962, 1185, 1963
BciT130I CCWGG 6 cut(s) 445, 969, 1053, 1374, 1710, 2340
BciVI GTATCC 2 cut(s) 480, 1963
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 2 cut(s) 1304, 1611
BfaI CTAG 3 cut(s) 816, 1956, 1986
BfmI CTRYAG 2 cut(s) 1524, 2949
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 717
BfuI GTATCC 2 cut(s) 480, 1963
BglII AGATCT 1 cut(s) 1811
BlnI CCTAGG 1 cut(s) 815
BmcAI AGTACT 1 cut(s) 2269
Bme1390I CCNGG 8 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340
Bme18I GGWCC 3 cut(s) 352, 1370, 2675
BmeT110I CYCGRG 2 cut(s) 656, 2087
BmgBI CACGTC 2 cut(s) 1423, 1474
BmgT120I GGNCC 4 cut(s) 352, 1370, 1499, 2675
BmiI GGNNCC 7 cut(s) 224, 490, 565, 884, 1284, 2082, 2325
BmrFI CCNGG 8 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340
BmrI ACTGGG 1 cut(s) 1583
BmsI GCATC 9 cut(s) 529, 721, 829, 1657, 1657, 1679, 2641, 2782, 2993
BmuI ACTGGG 1 cut(s) 1583
BpiI GAAGAC 1 cut(s) 1536
BpmI CTGGAG 2 cut(s) 1878, 2361
Bpu10I CCTNAGC 2 cut(s) 449, 1308
BpuEI CTTGAG 2 cut(s) 46, 2477
BpuMI CCSGG 2 cut(s) 1304, 1611
BsaAI YACGTR 1 cut(s) 1510
BsaBI GATNNNNATC 1 cut(s) 1416
BsaHI GRCGYC 2 cut(s) 360, 1950
BsaJI CCNNGG 8 cut(s) 203, 622, 791, 815, 1216, 1534, 2406, 2424
BsaWI WCCGGW 2 cut(s) 188, 343
BsaXI ACNNNNNCTCC 4 cut(s) 1566, 1596, 2004, 2034
Bse1I ACTGG 3 cut(s) 1342, 1589, 2327
Bse3DI GCAATG 2 cut(s) 1746, 2627
Bse8I GATNNNNATC 1 cut(s) 1416
BseBI CCWGG 6 cut(s) 445, 969, 1053, 1374, 1710, 2340
BseDI CCNNGG 8 cut(s) 203, 622, 791, 815, 1216, 1534, 2406, 2424
BseGI GGATG 9 cut(s) 220, 352, 514, 1417, 1648, 1670, 2635, 2797, 2811
BseJI GATNNNNATC 1 cut(s) 1416
BseMI GCAATG 2 cut(s) 1746, 2627
BseMII CTCAG 6 cut(s) 463, 1221, 1299, 1732, 2187, 2291
BseNI ACTGG 3 cut(s) 1342, 1589, 2327
BseRI GAGGAG 6 cut(s) 296, 481, 541, 1587, 2255, 2474
BseSI GKGCMC 2 cut(s) 831, 1659
BseX3I CGGCCG 2 cut(s) 206, 947
BseXI GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
BseYI CCCAGC 1 cut(s) 1451
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 1 cut(s) 265
Bsh1285I CGRYCG 2 cut(s) 209, 950
BshFI GGCC 9 cut(s) 208, 666, 949, 1500, 1836, 2225, 2405, 2565, 2890
BshNI GGYRCC 2 cut(s) 882, 2080
BsiEI CGRYCG 2 cut(s) 209, 950
BsiHKCI CYCGRG 2 cut(s) 656, 2087
BsiSI CCGG 4 cut(s) 189, 344, 1303, 1610
BslFI GGGAC 3 cut(s) 577, 1626, 2991
BsmBI CGTCTC 1 cut(s) 1429
BsmFI GGGAC 3 cut(s) 577, 1626, 2991
BsmI GAATGC 3 cut(s) 933, 2798, 2995
BsnI GGCC 9 cut(s) 208, 666, 949, 1500, 1836, 2225, 2405, 2565, 2890
BsoBI CYCGRG 2 cut(s) 656, 2087
Bsp1286I GDGCHC 4 cut(s) 89, 831, 1285, 1659
Bsp19I CCATGG 2 cut(s) 622, 1216
BspACI CCGC 6 cut(s) 209, 263, 320, 323, 950, 2887
BspANI GGCC 9 cut(s) 208, 666, 949, 1500, 1836, 2225, 2405, 2565, 2890
BspCNI CTCAG 6 cut(s) 462, 1222, 1300, 1731, 2188, 2290
BspFNI CGCG 1 cut(s) 265
BspHI TCATGA 3 cut(s) 520, 1480, 1870
BspLI GGNNCC 7 cut(s) 224, 490, 565, 884, 1284, 2082, 2325
BspMI ACCTGC 1 cut(s) 717
BspPI GGATC 6 cut(s) 525, 830, 2148, 2458, 2728, 2873
BspT107I GGYRCC 2 cut(s) 882, 2080
BspTI CTTAAG 1 cut(s) 329
BsrDI GCAATG 2 cut(s) 1746, 2627
BsrI ACTGG 3 cut(s) 1342, 1589, 2327
BssECI CCNNGG 8 cut(s) 203, 622, 791, 815, 1216, 1534, 2406, 2424
BssNI GRCGYC 2 cut(s) 360, 1950
BssSI CACGAG 2 cut(s) 162, 1724
BssT1I CCWWGG 4 cut(s) 622, 815, 1216, 2424
Bst2BI CACGAG 2 cut(s) 162, 1724
Bst2UI CCWGG 6 cut(s) 445, 969, 1053, 1374, 1710, 2340
Bst6I CTCTTC 2 cut(s) 402, 2276
BstACI GRCGYC 2 cut(s) 360, 1950
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 1654
BstBAI YACGTR 1 cut(s) 1510
BstC8I GCNNGC 4 cut(s) 22, 1498, 2551, 2563
BstDEI CTNAG 7 cut(s) 449, 1230, 1308, 1718, 2196, 2277, 2621
BstDSI CCRYGG 4 cut(s) 622, 791, 1216, 1534
BstENI CCTNNNNNAGG 2 cut(s) 732, 1989
BstF5I GGATG 9 cut(s) 220, 352, 514, 1417, 1648, 1670, 2635, 2797, 2811
BstFNI CGCG 1 cut(s) 265
BstHHI GCGC 1 cut(s) 544
BstMCI CGRYCG 2 cut(s) 209, 950
BstMWI GCNNNNNNNGC 8 cut(s) 208, 672, 826, 1313, 1654, 2303, 2792, 2904
BstNI CCWGG 6 cut(s) 445, 969, 1053, 1374, 1710, 2340
BstNSI RCATGY 4 cut(s) 24, 1191, 1328, 1607
BstSCI CCNGG 8 cut(s) 443, 967, 1051, 1302, 1372, 1609, 1708, 2338
BstSFI CTRYAG 2 cut(s) 1524, 2949
BstSLI GKGCMC 2 cut(s) 831, 1659
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
BstV2I GAAGAC 1 cut(s) 1536
BstX2I RGATCY 4 cut(s) 1811, 2463, 2720, 2878
BstXI CCANNNNNNTGG 3 cut(s) 403, 485, 1694
BstYI RGATCY 4 cut(s) 1811, 2463, 2720, 2878
BstZI CGGCCG 2 cut(s) 206, 947
BsuI GTATCC 2 cut(s) 480, 1963
BsuRI GGCC 9 cut(s) 208, 666, 949, 1500, 1836, 2225, 2405, 2565, 2890
BtgI CCRYGG 4 cut(s) 622, 791, 1216, 1534
BtgZI GCGATG 2 cut(s) 698, 1767
BtrI CACGTC 2 cut(s) 1423, 1474
BtsCI GGATG 9 cut(s) 220, 352, 514, 1417, 1648, 1670, 2635, 2797, 2811
BtsIMutI CAGTG 4 cut(s) 1099, 1105, 1596, 2334
BveI ACCTGC 1 cut(s) 717
Cac8I GCNNGC 4 cut(s) 22, 1498, 2551, 2563
CciI TCATGA 3 cut(s) 520, 1480, 1870
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 4 cut(s) 352, 1370, 1499, 2675
CseI GACGC 2 cut(s) 368, 1939
Csp6I GTAC 6 cut(s) 334, 883, 905, 1511, 2268, 2834
CspCI CAANNNNNGTGG 2 cut(s) 1275, 1310
CviQI GTAC 6 cut(s) 334, 883, 905, 1511, 2268, 2834
DdeI CTNAG 7 cut(s) 449, 1230, 1308, 1718, 2196, 2277, 2621
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 5 cut(s) 206, 947, 1834, 2223, 2563
EagI CGGCCG 2 cut(s) 206, 947
Eam1104I CTCTTC 2 cut(s) 402, 2276
EarI CTCTTC 2 cut(s) 402, 2276
EclXI CGGCCG 2 cut(s) 206, 947
Eco130I CCWWGG 4 cut(s) 622, 815, 1216, 2424
Eco24I GRGCYC 2 cut(s) 89, 1285
Eco32I GATATC 2 cut(s) 690, 1715
Eco47I GGWCC 3 cut(s) 352, 1370, 2675
Eco52I CGGCCG 2 cut(s) 206, 947
Eco57I CTGAAG 2 cut(s) 591, 954
Eco88I CYCGRG 2 cut(s) 656, 2087
EcoNI CCTNNNNNAGG 2 cut(s) 732, 1989
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 2705
EcoRII CCWGG 6 cut(s) 443, 967, 1051, 1372, 1708, 2338
EcoRV GATATC 2 cut(s) 690, 1715
EcoT14I CCWWGG 4 cut(s) 622, 815, 1216, 2424
EcoT22I ATGCAT 2 cut(s) 1650, 1672
EcoT38I GRGCYC 2 cut(s) 89, 1285
ErhI CCWWGG 4 cut(s) 622, 815, 1216, 2424
Esp3I CGTCTC 1 cut(s) 1429
FalI AAGNNNNNCTT 2 cut(s) 1031, 1063
FaqI GGGAC 3 cut(s) 577, 1626, 2991
FauNDI CATATG 3 cut(s) 1546, 2488, 2989
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 1 cut(s) 72
FokI GGATG 9 cut(s) 227, 359, 521, 1424, 1635, 1657, 2622, 2804, 2818
FriOI GRGCYC 2 cut(s) 89, 1285
FspBI CTAG 3 cut(s) 816, 1956, 1986
GlaI GCGC 1 cut(s) 543
GsaI CCCAGC 1 cut(s) 1455
GsuI CTGGAG 2 cut(s) 1878, 2361
HaeIII GGCC 9 cut(s) 208, 666, 949, 1500, 1836, 2225, 2405, 2565, 2890
HapII CCGG 4 cut(s) 189, 344, 1303, 1610
HgaI GACGC 2 cut(s) 368, 1939
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 2 cut(s) 360, 1950
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HindIII AAGCTT 2 cut(s) 1839, 2165
HpaII CCGG 4 cut(s) 189, 344, 1303, 1610
HphI GGTGA 8 cut(s) 433, 443, 1046, 1242, 2014, 2075, 2144, 2937
Hpy166II GTNNAC 6 cut(s) 73, 1513, 1585, 1982, 2332, 2771
Hpy8I GTNNAC 6 cut(s) 73, 1513, 1585, 1982, 2332, 2771
Hpy99I CGWCG 1 cut(s) 2769
HpyCH4IV ACGT 4 cut(s) 1422, 1473, 1509, 2584
HpyF10VI GCNNNNNNNGC 8 cut(s) 208, 672, 826, 1313, 1654, 2303, 2792, 2904
HpyF3I CTNAG 7 cut(s) 449, 1230, 1308, 1718, 2196, 2277, 2621
HpySE526I ACGT 4 cut(s) 1422, 1473, 1509, 2584
Hsp92I GRCGYC 2 cut(s) 360, 1950
HspAI GCGC 1 cut(s) 542
KpnI GGTACC 1 cut(s) 886
Ksp22I TGATCA 1 cut(s) 49
LmnI GCTCC 8 cut(s) 228, 304, 494, 793, 1022, 1288, 1687, 2492
Lsp1109I GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
LweI GCATC 9 cut(s) 529, 721, 829, 1657, 1657, 1679, 2641, 2782, 2993
MaeI CTAG 3 cut(s) 816, 1956, 1986
MaeII ACGT 4 cut(s) 1422, 1473, 1509, 2584
MaeIII GTNAC 6 cut(s) 391, 1055, 1220, 1951, 2132, 2698
MboII GAAGA 8 cut(s) 419, 947, 1239, 1541, 1547, 1821, 2135, 2293
MfeI CAATTG 2 cut(s) 45, 2757
MflI RGATCY 4 cut(s) 1811, 2463, 2720, 2878
MhlI GDGCHC 4 cut(s) 89, 831, 1285, 1659
MlsI TGGCCA 2 cut(s) 1836, 2225
MluNI TGGCCA 2 cut(s) 1836, 2225
MlyI GAGTC 5 cut(s) 41, 599, 661, 805, 2973
MmeI TCCRAC 3 cut(s) 15, 884, 1947
Mox20I TGGCCA 2 cut(s) 1836, 2225
Mph1103I ATGCAT 2 cut(s) 1650, 1672
MroXI GAANNNNTTC 1 cut(s) 1902
MscI TGGCCA 2 cut(s) 1836, 2225
MseI TTAA 6 cut(s) 168, 330, 554, 1692, 2715, 2913
MslI CAYNNNNRTG 3 cut(s) 1602, 2640, 2969
Msp20I TGGCCA 2 cut(s) 1836, 2225
MspA1I CMGCKG 2 cut(s) 2553, 2887
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 4 cut(s) 189, 344, 1303, 1610
MspR9I CCNGG 8 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340
MunI CAATTG 2 cut(s) 45, 2757
Mva1269I GAATGC 3 cut(s) 933, 2798, 2995
MvaI CCWGG 6 cut(s) 445, 969, 1053, 1374, 1710, 2340
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 8 cut(s) 208, 672, 826, 1313, 1654, 2303, 2792, 2904
NciI CCSGG 2 cut(s) 1304, 1611
NcoI CCATGG 2 cut(s) 622, 1216
NdeI CATATG 3 cut(s) 1546, 2488, 2989
NlaIV GGNNCC 7 cut(s) 224, 490, 565, 884, 1284, 2082, 2325
NmeAIII GCCGAG 2 cut(s) 184, 2431
NmuCI GTSAC 3 cut(s) 391, 1951, 2132
NsiI ATGCAT 2 cut(s) 1650, 1672
NspI RCATGY 4 cut(s) 24, 1191, 1328, 1607
PaeI GCATGC 1 cut(s) 24
PaeR7I CTCGAG 2 cut(s) 656, 2087
PagI TCATGA 3 cut(s) 520, 1480, 1870
PciI ACATGT 2 cut(s) 1187, 1324
PcsI WCGNNNNNNNCGW 1 cut(s) 2770
PctI GAATGC 3 cut(s) 933, 2798, 2995
PdmI GAANNNNTTC 1 cut(s) 1902
PfeI GAWTC 8 cut(s) 958, 1244, 1267, 1765, 1778, 2273, 2357, 2371
PflFI GACNNNGTC 1 cut(s) 1949
PflMI CCANNNNNTGG 1 cut(s) 628
PfoI TCCNGGA 2 cut(s) 1708, 2338
PleI GAGTC 5 cut(s) 40, 598, 660, 804, 2973
PpsI GAGTC 5 cut(s) 40, 598, 660, 804, 2973
Ppu21I YACGTR 1 cut(s) 1510
PpuMI RGGWCCY 1 cut(s) 352
PscI ACATGT 2 cut(s) 1187, 1324
PshBI ATTAAT 1 cut(s) 1692
PsiI TTATAA 2 cut(s) 705, 2045
Psp1406I AACGTT 1 cut(s) 2584
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 6 cut(s) 443, 967, 1051, 1372, 1708, 2338
PspFI CCCAGC 1 cut(s) 1451
PspGI CCWGG 6 cut(s) 443, 967, 1051, 1372, 1708, 2338
PspN4I GGNNCC 7 cut(s) 224, 490, 565, 884, 1284, 2082, 2325
PspPI GGNCC 4 cut(s) 352, 1370, 1499, 2675
PspPPI RGGWCCY 1 cut(s) 352
PsrI GAACNNNNNNTAC 2 cut(s) 1340, 1372
PsuI RGATCY 4 cut(s) 1811, 2463, 2720, 2878
PsyI GACNNNGTC 1 cut(s) 1949
PvuII CAGCTG 1 cut(s) 2553
RsaI GTAC 6 cut(s) 335, 884, 906, 1512, 2269, 2835
RsaNI GTAC 6 cut(s) 334, 883, 905, 1511, 2268, 2834
RseI CAYNNNNRTG 3 cut(s) 1602, 2640, 2969
SaqAI TTAA 6 cut(s) 168, 330, 554, 1692, 2715, 2913
Sau96I GGNCC 4 cut(s) 352, 1370, 1499, 2675
ScaI AGTACT 1 cut(s) 2269
SchI GAGTC 5 cut(s) 41, 599, 661, 805, 2973
ScrFI CCNGG 8 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340
SduI GDGCHC 4 cut(s) 89, 831, 1285, 1659
SfaNI GCATC 9 cut(s) 529, 721, 829, 1657, 1657, 1679, 2641, 2782, 2993
SfcI CTRYAG 2 cut(s) 1524, 2949
Sfr274I CTCGAG 2 cut(s) 656, 2087
SinI GGWCC 3 cut(s) 352, 1370, 2675
SlaI CTCGAG 2 cut(s) 656, 2087
SmiMI CAYNNNNRTG 3 cut(s) 1602, 2640, 2969
SmlI CTYRAG 5 cut(s) 25, 329, 656, 2087, 2456
SmoI CTYRAG 5 cut(s) 25, 329, 656, 2087, 2456
SphI GCATGC 1 cut(s) 24
SsiI CCGC 6 cut(s) 209, 263, 320, 323, 950, 2887
SspMI CTAG 3 cut(s) 816, 1956, 1986
StyD4I CCNGG 8 cut(s) 443, 967, 1051, 1302, 1372, 1609, 1708, 2338
StyI CCWWGG 4 cut(s) 622, 815, 1216, 2424
TaiI ACGT 4 cut(s) 1425, 1476, 1512, 2587
TaqI TCGA 4 cut(s) 657, 1083, 1143, 2088
TaqII GACCGA 1 cut(s) 1500
TatI WGTACW 2 cut(s) 2267, 2833
TauI GCSGC 4 cut(s) 211, 323, 952, 2890
TfiI GAWTC 8 cut(s) 958, 1244, 1267, 1765, 1778, 2273, 2357, 2371
Tru1I TTAA 6 cut(s) 168, 330, 554, 1692, 2715, 2913
Tru9I TTAA 6 cut(s) 168, 330, 554, 1692, 2715, 2913
TscAI CASTG 4 cut(s) 1099, 1112, 1596, 2334
TseFI GTSAC 3 cut(s) 391, 1951, 2132
TseI GCWGC 6 cut(s) 55, 58, 580, 1751, 2306, 2795
Tsp45I GTSAC 3 cut(s) 391, 1951, 2132
TspGWI ACGGA 4 cut(s) 262, 780, 1584, 1821
TspRI CASTG 4 cut(s) 1099, 1112, 1596, 2334
Tth111I GACNNNGTC 1 cut(s) 1949
Van91I CCANNNNNTGG 1 cut(s) 628
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 3 cut(s) 352, 1370, 2675
VspI ATTAAT 1 cut(s) 1692
XagI CCTNNNNNAGG 2 cut(s) 732, 1989
XapI RAATTY 6 cut(s) 170, 569, 1381, 2098, 2157, 2705
XceI RCATGY 4 cut(s) 24, 1191, 1328, 1607
XcmI CCANNNNNNNNNTGG 2 cut(s) 1448, 2971
XhoI CTCGAG 2 cut(s) 656, 2087
XmaJI CCTAGG 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 1902
XspI CTAG 3 cut(s) 816, 1956, 1986
ZrmI AGTACT 1 cut(s) 2269
Zsp2I ATGCAT 2 cut(s) 1650, 1672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.