Rroxscaffold_5G00347950

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005070
Physical Location & Seq
Forward (+)
19769533 .. 19775485
5953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347950.1

Sequence Viewer

Length: 3084 bp
ATGCCAAATTCCTCCGGAGGAGATAAATCAGATGCTAGCAAGAGGAGAAACGCGAAAGGCAGTTTCTCCGGTAGTATATTGCCTCGGCCGCAGGATGGGAGGCTCCGATATGTGGGTGGGGAGACACGAATTGTTAGTGTTCCGCGAGACATTAGGTATGAGGAGTTGATGAGCAAGATGAGGGAGCTTTATGAAGGGGCGGCGGTGCTTAAGTACCAGCAACCGGATGAGGACCTTGACGCCCTTGTCTCGGTTGTGAATGATGATGATGTGACCAACATGATGGAAGAGTATGATAAGTTGGGGTCGGGTGATGGTTTCACCAGGCTCAGGATTTTTTTGTTTTCGTATCCCGACCAAGATGGTGGCTCCTCACACTATGACGGGGATGAAAGGGATCATGAGAGGAGGTATGTTGATGCTCTCAATAACTTAAATGATGGTTCCGAATTTAGGAAGCAGCATTTAGAGTCCCCTGTTATCACTTCAGTTGATGATCTCCATGGAGTTGAACAGTTCTTTAGTCCAATGAGTCTCGAGGGTGGCCTCCATAGCCAGAGAAACGAGATATCCATGTCTCATTATAACCTGCATCGCCTCAAAATACCTCATATAGGTTCAGGGCAACACCATCAGCCCATTAGCCAAAGGTATAACGACATGGAAGCTCCGTGGAGTCCTGCATTCTATTCTCCTAGGCATCATGGGCACATTGATCCAAGACCAATGGCAGAGTTTCCAACTTCTCCTACCTCTGCACGGTACCGTATGCCATTTGCAGATGTACCTGATAAATGTTCAGATAGAATGCCTGAAGAATATGCACGGCCGCCATTGAATCATCAGCCTGGATATGAACACCAACCACAGTATTCTGATAATGCTGTATGGCTTCCAAGTGGAGCTATATCTGGTGAGAAGTCAGGCTTTCCAGGTAACATTTTTCATGGAAACAATGTTCTCGAAGGAAACAGTGTCTGTGAACACTGTCGGGTATGTTTCCAAAGAAACCAACCACATTTCGAGCAACCCAACGTGGTCAATGGATTTCATCAGGTTGCTAATACATGTGCTGATTGCCGTCCAAAGTTGCACCATGGTTACACAACTGAGCAGAATAACGATTCTTCATCTCTATATAATGAGAATCAAAATCAATGGGTTCCACACCATTTGAATAGCCGGGCTGAGGAAGCAAGATCACATGTCTCTGGAACTGGAAAATTGAACGATCACTACATTGTAGATGGTCCTGGCATGAATTTGCCTCTTGTTCATAATAATATGGTGGATGTCCATCACGTCTCTACCAATTTTGTCCATCAACGAGCTGGGTCTGAAATGGGAAATGACGTGTTTCATGACCGACCTGTGGCTGGCCCACCCCACGTTCACATTCCTCCTGTAGAAGACCGTGGCGTTCGATATGGAAATCTTCCTTATGCTTACGGAGGAGATAATGTTTACCCAGTGTCTCATGGACATGCACCGGGACATGGAGTGTGGAGAAATGTTCATAGCACAATGCACCCTGTGCCCCCTTACGATGCATCCAATTCAGCTCCCCAAATTAATGGTTCTGTCAATCCAGGATATCTCAGACACGAGGGTAGTCCAAGATTTTGCATTGCAGCAGACAATCAGAATCTTTGGGTTGAATCATCGCAGAAGGTGTTAGGTTTTGACGGAAAATCTGTTCTGGACTATTCTTATGGCCAAGCTTTGAACTTGAACCCTAACACACTTGCTCATGAAAATCATCATCAATTTCCTCCAGAACTCATTCAACCCACACCTGATATAGTAAGTTCTGCTACCCCTTTGGACGGCGTCACTAGAGTTATGTTGGATACAGAGAGTTTACCTAGAGAAGGAAGGGAGGTGATTGATGGAGAGAAGGTTGAGAACTCTGATATGCAGCTCGCAGAAGAAAGTGGTGACAAGGATCATTCTACTCCTGAAATTTCAAAGCTTTCAGCTAACAATTTGAGTTTTGTGCCTGAGTTGATTGCTTCTGTAAAAAAAGTGGCCATATATGGGGTAGAGGAGGTAAAAGCAAAAGTTGAGGAAAGTACTGATTCTCAGAAGAGTAATCCGATTGCCAATGAAGCTGCAACAAACAGTTTGGAACCAGTGAACACTCCTGGAGATGGGGAAGTGGATTCTGATAGTGACAATCTGAATAATTCCAAAATTGAGCCAACAAAGGCCGAGGCTGAAGCTATTTCCAAGGGATTGCAGACAATAAAGAATGATGATCTAGAGGAGATCCGAGAATTAGGTTCTGGAACATATGGGGCTGTTTTTCATGGGAAGTGGAAGGGTTCAGATGTAGCAATAAAAAGAATAAAAGCCAGCTGTTTTGCTGGCCGACCTTCTGAAAGAGAGCGTTTGATTGCTGATTTCTGGAAGGAGGCTTTGATACTTAGTTCATTGCATCATCCAAATGTTGTTTCTTTCTATGGTATTGTGCGTGATGGTCCTGATGGCTCTTTAGCAACTGTTACTGAATTCATGGTTAATGGATCTTTGAAACAGTTTTTGCAGAAAAAAGACAGAACAATTGATCGTCGTAAACGATTGATTATAGCTATGGATGCTGCATTCGGGATGGAGTATCTGCATGGAAAAAATATAGTACATTTTGATTTGAAGTGTGAAAATCTGTTGGTAAATATGAGAGATCCACAGCGGCCTGTATGCAAGATTGGTGATTTGGGATTGTCAAAAGTGAAACAACAGACATTAGTGTCTGGAGGTGTTCGTGGAACTTTGCCCTGGATGGCACCAGAGCTTCTTAGTGGGAAAAGTCATATGGTGACAGAGAAGATTGATGTTTACTCATTTGGGATTGTTATGTGGGAACTGCTTACTGGCGATGAACCCTATACAGATATGCATTGTGCTTCTATAATTGGAGGAATTGTGAACAACACTTTACGTCCTCAAATTCCAACATGGTGTGATCCTGAATGGAAGTCTTTGATGGAAAGTTGTTGGGGCTCTGAGCCTGCTCAGAGGCCATCATTTTCTGAGATCTCTCAGAAGCTAAGGAATATGGCTGCTGCAATGAATATGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1027

Amino Acids

114.4

Weight (kDa)

5.68

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 32 - 115 1.2e-17 PB1 domain
Pkinase PF00069 755 - 1015 3.8e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 756 - 1017 3.6e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AasI GACNNNNNNGTC 2 cut(s) 245, 2749
Acc36I ACCTGC 1 cut(s) 597
Acc65I GGTACC 1 cut(s) 762
AccB1I GGYRCC 2 cut(s) 762, 2785
AccII CGCG 2 cut(s) 53, 145
AccIII TCCGGA 1 cut(s) 14
AciI CCGC 6 cut(s) 89, 143, 200, 203, 830, 2692
AclWI GGATC 7 cut(s) 405, 710, 1953, 2263, 2533, 2678, 2960
AcoI YGGCCR 5 cut(s) 86, 827, 1714, 2028, 2368
AcsI RAATTY 6 cut(s) 7, 449, 1261, 1962, 2510, 2949
AcuI CTGAAG 3 cut(s) 471, 834, 2238
AcyI GRCGYC 2 cut(s) 240, 1830
AdeI CACNNNGTG 1 cut(s) 1534
AfaI GTAC 5 cut(s) 215, 764, 786, 2074, 2640
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 3 cut(s) 1067, 1204, 1353
AjiI CACGTC 2 cut(s) 1303, 1354
AjnI CCWGG 7 cut(s) 323, 847, 931, 1252, 1588, 2143, 2777
AjuI GAANNNNNNNTTGG 2 cut(s) 2437, 2469
AloI GAACNNNNNNTCC 4 cut(s) 942, 974, 1680, 1712
Alw26I GTCTC 8 cut(s) 116, 141, 253, 539, 582, 1213, 1309, 1479
AlwI GGATC 7 cut(s) 405, 710, 1953, 2263, 2533, 2678, 2960
AlwNI CAGNNNCTG 1 cut(s) 978
Ama87I CYCGRG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 14
ApeKI GCWGC 7 cut(s) 460, 1631, 1918, 2111, 2600, 3062, 3065
ApoI RAATTY 6 cut(s) 7, 449, 1261, 1962, 2510, 2949
AseI ATTAAT 1 cut(s) 1572
Asp700I GAANNNNTTC 1 cut(s) 1782
Asp718I GGTACC 1 cut(s) 762
AspA2I CCTAGG 1 cut(s) 695
AspS9I GGNCC 4 cut(s) 232, 1250, 1379, 2480
AsuC2I CCSGG 2 cut(s) 1184, 1491
AsuHPI GGTGA 7 cut(s) 313, 323, 926, 1894, 1949, 2723, 2830
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 3 cut(s) 232, 1250, 2480
AvrII CCTAGG 1 cut(s) 695
BaeGI GKGCMC 2 cut(s) 711, 1539
BalI TGGCCA 2 cut(s) 1716, 2030
BanI GGYRCC 2 cut(s) 762, 2785
BanII GRGCYC 1 cut(s) 3005
BauI CACGAG 1 cut(s) 1604
BbsI GAAGAC 1 cut(s) 1416
BbvCI CCTCAGC 1 cut(s) 1188
BbvI GCAGC 7 cut(s) 472, 1643, 1930, 2098, 2587, 3049, 3052
BceAI ACGGC 3 cut(s) 842, 1065, 1843
BciT130I CCWGG 7 cut(s) 325, 849, 933, 1254, 1590, 2145, 2779
BciVI GTATCC 2 cut(s) 360, 1843
BcnI CCSGG 2 cut(s) 1184, 1491
BcoDI GTCTC 8 cut(s) 116, 141, 253, 539, 582, 1213, 1309, 1479
BfaI CTAG 5 cut(s) 36, 696, 1836, 1866, 2261
BfmI CTRYAG 1 cut(s) 1404
BfrI CTTAAG 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 597
BfuI GTATCC 2 cut(s) 360, 1843
BglII AGATCT 1 cut(s) 3036
BlnI CCTAGG 1 cut(s) 695
BmcAI AGTACT 1 cut(s) 2074
Bme1390I CCNGG 9 cut(s) 325, 849, 933, 1184, 1254, 1491, 1590, 2145, 2779
Bme18I GGWCC 3 cut(s) 232, 1250, 2480
BmeT110I CYCGRG 1 cut(s) 536
BmgBI CACGTC 2 cut(s) 1303, 1354
BmgT120I GGNCC 4 cut(s) 232, 1250, 1379, 2480
BmiI GGNNCC 7 cut(s) 104, 370, 445, 764, 1164, 2130, 2787
BmrFI CCNGG 9 cut(s) 325, 849, 933, 1184, 1254, 1491, 1590, 2145, 2779
BmrI ACTGGG 1 cut(s) 1463
BmsI GCATC 8 cut(s) 22, 409, 601, 709, 1537, 1559, 2446, 2587
BmtI GCTAGC 1 cut(s) 39
BmuI ACTGGG 1 cut(s) 1463
BpiI GAAGAC 1 cut(s) 1416
BpmI CTGGAG 3 cut(s) 1758, 2166, 2775
Bpu10I CCTNAGC 3 cut(s) 329, 1188, 3050
BpuMI CCSGG 2 cut(s) 1184, 1491
BsaBI GATNNNNATC 1 cut(s) 1296
BsaHI GRCGYC 2 cut(s) 240, 1830
BsaJI CCNNGG 9 cut(s) 83, 502, 671, 695, 1096, 1414, 2211, 2229, 2777
BsaWI WCCGGW 3 cut(s) 14, 68, 223
BsaXI ACNNNNNCTCC 6 cut(s) 1446, 1476, 1884, 1914, 2910, 2940
Bse1I ACTGG 4 cut(s) 1222, 1469, 2132, 2878
Bse3DI GCAATG 3 cut(s) 1626, 2432, 3075
Bse8I GATNNNNATC 1 cut(s) 1296
BseAI TCCGGA 1 cut(s) 14
BseBI CCWGG 7 cut(s) 325, 849, 933, 1254, 1590, 2145, 2779
BseDI CCNNGG 9 cut(s) 83, 502, 671, 695, 1096, 1414, 2211, 2229, 2777
BseGI GGATG 9 cut(s) 100, 232, 394, 1297, 1550, 2440, 2602, 2616, 2787
BseJI GATNNNNATC 1 cut(s) 1296
BseMI GCAATG 3 cut(s) 1626, 2432, 3075
BseNI ACTGG 4 cut(s) 1222, 1469, 2132, 2878
BseRI GAGGAG 8 cut(s) 33, 58, 176, 361, 421, 1467, 2060, 2279
BseSI GKGCMC 2 cut(s) 711, 1539
BseX3I CGGCCG 2 cut(s) 86, 827
BseXI GCAGC 7 cut(s) 472, 1643, 1930, 2098, 2587, 3049, 3052
BseYI CCCAGC 1 cut(s) 1331
BsgI GTGCAG 1 cut(s) 741
Bsh1236I CGCG 2 cut(s) 53, 145
Bsh1285I CGRYCG 2 cut(s) 89, 830
BshNI GGYRCC 2 cut(s) 762, 2785
BsiEI CGRYCG 2 cut(s) 89, 830
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 5 cut(s) 15, 69, 224, 1183, 1490
BslFI GGGAC 2 cut(s) 457, 1506
BsmAI GTCTC 8 cut(s) 116, 141, 253, 539, 582, 1213, 1309, 1479
BsmBI CGTCTC 1 cut(s) 1309
BsmFI GGGAC 2 cut(s) 457, 1506
BsmI GAATGC 3 cut(s) 683, 813, 2603
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 3 cut(s) 711, 1539, 3005
Bsp13I TCCGGA 1 cut(s) 14
Bsp19I CCATGG 2 cut(s) 502, 1096
BspACI CCGC 6 cut(s) 89, 143, 200, 203, 830, 2692
BspEI TCCGGA 1 cut(s) 14
BspFNI CGCG 2 cut(s) 53, 145
BspHI TCATGA 3 cut(s) 400, 1360, 1750
BspLI GGNNCC 7 cut(s) 104, 370, 445, 764, 1164, 2130, 2787
BspMI ACCTGC 1 cut(s) 597
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 7 cut(s) 405, 710, 1953, 2263, 2533, 2678, 2960
BspT107I GGYRCC 2 cut(s) 762, 2785
BspTI CTTAAG 1 cut(s) 209
BsrDI GCAATG 3 cut(s) 1626, 2432, 3075
BsrI ACTGG 4 cut(s) 1222, 1469, 2132, 2878
BssECI CCNNGG 9 cut(s) 83, 502, 671, 695, 1096, 1414, 2211, 2229, 2777
BssNI GRCGYC 2 cut(s) 240, 1830
BssSI CACGAG 1 cut(s) 1604
BssT1I CCWWGG 4 cut(s) 502, 695, 1096, 2229
Bst2BI CACGAG 1 cut(s) 1604
Bst2UI CCWGG 7 cut(s) 325, 849, 933, 1254, 1590, 2145, 2779
Bst6I CTCTTC 2 cut(s) 282, 2081
BstACI GRCGYC 2 cut(s) 240, 1830
BstAFI CTTAAG 1 cut(s) 209
BstAPI GCANNNNNTGC 1 cut(s) 1534
BstC8I GCNNGC 6 cut(s) 37, 1378, 1923, 2356, 2368, 3012
BstDSI CCRYGG 4 cut(s) 502, 671, 1096, 1414
BstENI CCTNNNNNAGG 2 cut(s) 612, 1869
BstF5I GGATG 9 cut(s) 100, 232, 394, 1297, 1550, 2440, 2602, 2616, 2787
BstFNI CGCG 2 cut(s) 53, 145
BstMAI GTCTC 8 cut(s) 116, 141, 253, 539, 582, 1213, 1309, 1479
BstMCI CGRYCG 2 cut(s) 89, 830
BstMWI GCNNNNNNNGC 7 cut(s) 88, 552, 706, 1193, 1534, 2108, 2597
BstNI CCWGG 7 cut(s) 325, 849, 933, 1254, 1590, 2145, 2779
BstNSI RCATGY 3 cut(s) 1071, 1208, 1487
BstSCI CCNGG 9 cut(s) 323, 847, 931, 1182, 1252, 1489, 1588, 2143, 2777
BstSFI CTRYAG 1 cut(s) 1404
BstSLI GKGCMC 2 cut(s) 711, 1539
BstUI CGCG 2 cut(s) 53, 145
BstV1I GCAGC 7 cut(s) 472, 1643, 1930, 2098, 2587, 3049, 3052
BstV2I GAAGAC 1 cut(s) 1416
BstX2I RGATCY 4 cut(s) 2268, 2525, 2683, 3036
BstXI CCANNNNNNTGG 3 cut(s) 283, 365, 1574
BstYI RGATCY 4 cut(s) 2268, 2525, 2683, 3036
BstZI CGGCCG 2 cut(s) 86, 827
BsuI GTATCC 2 cut(s) 360, 1843
BtgI CCRYGG 4 cut(s) 502, 671, 1096, 1414
BtgZI GCGATG 3 cut(s) 578, 1647, 2892
BtrI CACGTC 2 cut(s) 1303, 1354
BtsCI GGATG 9 cut(s) 100, 232, 394, 1297, 1550, 2440, 2602, 2616, 2787
BtsIMutI CAGTG 4 cut(s) 979, 985, 1476, 2139
BveI ACCTGC 1 cut(s) 597
Cac8I GCNNGC 6 cut(s) 37, 1378, 1923, 2356, 2368, 3012
CaiI CAGNNNCTG 1 cut(s) 978
CciI TCATGA 3 cut(s) 400, 1360, 1750
Cfr13I GGNCC 4 cut(s) 232, 1250, 1379, 2480
CseI GACGC 2 cut(s) 248, 1819
Csp6I GTAC 5 cut(s) 214, 763, 785, 2073, 2639
CspCI CAANNNNNGTGG 2 cut(s) 1155, 1190
CviQI GTAC 5 cut(s) 214, 763, 785, 2073, 2639
DraIII CACNNNGTG 1 cut(s) 1534
DrdI GACNNNNNNGTC 2 cut(s) 245, 2749
DseDI GACNNNNNNGTC 2 cut(s) 245, 2749
EaeI YGGCCR 5 cut(s) 86, 827, 1714, 2028, 2368
EagI CGGCCG 2 cut(s) 86, 827
Eam1104I CTCTTC 2 cut(s) 282, 2081
EarI CTCTTC 2 cut(s) 282, 2081
EclXI CGGCCG 2 cut(s) 86, 827
Eco130I CCWWGG 4 cut(s) 502, 695, 1096, 2229
Eco24I GRGCYC 1 cut(s) 3005
Eco32I GATATC 2 cut(s) 570, 1595
Eco47I GGWCC 3 cut(s) 232, 1250, 2480
Eco52I CGGCCG 2 cut(s) 86, 827
Eco57I CTGAAG 3 cut(s) 471, 834, 2238
Eco88I CYCGRG 1 cut(s) 536
EcoNI CCTNNNNNAGG 2 cut(s) 612, 1869
EcoO109I RGGNCCY 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 2510
EcoRII CCWGG 7 cut(s) 323, 847, 931, 1252, 1588, 2143, 2777
EcoRV GATATC 2 cut(s) 570, 1595
EcoT14I CCWWGG 4 cut(s) 502, 695, 1096, 2229
EcoT22I ATGCAT 2 cut(s) 1552, 2901
EcoT38I GRGCYC 1 cut(s) 3005
ErhI CCWWGG 4 cut(s) 502, 695, 1096, 2229
Esp3I CGTCTC 1 cut(s) 1309
FalI AAGNNNNNCTT 2 cut(s) 911, 943
FaqI GGGAC 2 cut(s) 457, 1506
FauNDI CATATG 2 cut(s) 2293, 2814
FokI GGATG 9 cut(s) 107, 239, 401, 1304, 1537, 2427, 2609, 2623, 2794
FriOI GRGCYC 1 cut(s) 3005
FspBI CTAG 5 cut(s) 36, 696, 1836, 1866, 2261
GsaI CCCAGC 1 cut(s) 1335
GsuI CTGGAG 3 cut(s) 1758, 2166, 2775
HapII CCGG 5 cut(s) 15, 69, 224, 1183, 1490
HgaI GACGC 2 cut(s) 248, 1819
Hin1I GRCGYC 2 cut(s) 240, 1830
HindIII AAGCTT 2 cut(s) 1719, 1970
HpaII CCGG 5 cut(s) 15, 69, 224, 1183, 1490
HphI GGTGA 7 cut(s) 313, 323, 926, 1894, 1949, 2723, 2830
Hpy166II GTNNAC 8 cut(s) 983, 1393, 1465, 1862, 2137, 2576, 2839, 2929
Hpy8I GTNNAC 8 cut(s) 983, 1393, 1465, 1862, 2137, 2576, 2839, 2929
Hpy99I CGWCG 1 cut(s) 2574
HpyAV CCTTC 9 cut(s) 188, 959, 1663, 1865, 1869, 1891, 2314, 2385, 2404
HpyCH4IV ACGT 5 cut(s) 1035, 1302, 1353, 1389, 2941
HpyF10VI GCNNNNNNNGC 7 cut(s) 88, 552, 706, 1193, 1534, 2108, 2597
HpySE526I ACGT 5 cut(s) 1035, 1302, 1353, 1389, 2941
Hsp92I GRCGYC 2 cut(s) 240, 1830
Kpn2I TCCGGA 1 cut(s) 14
KpnI GGTACC 1 cut(s) 766
LmnI GCTCC 6 cut(s) 108, 184, 374, 673, 902, 1567
Lsp1109I GCAGC 7 cut(s) 472, 1643, 1930, 2098, 2587, 3049, 3052
LweI GCATC 8 cut(s) 22, 409, 601, 709, 1537, 1559, 2446, 2587
MaeI CTAG 5 cut(s) 36, 696, 1836, 1866, 2261
MaeII ACGT 5 cut(s) 1035, 1302, 1353, 1389, 2941
MaeIII GTNAC 8 cut(s) 271, 935, 1100, 1831, 1937, 2171, 2503, 2818
MboII GAAGA 8 cut(s) 299, 827, 1119, 1421, 1427, 1940, 2098, 2839
MfeI CAATTG 1 cut(s) 2562
MflI RGATCY 4 cut(s) 2268, 2525, 2683, 3036
MhlI GDGCHC 3 cut(s) 711, 1539, 3005
MlsI TGGCCA 2 cut(s) 1716, 2030
MluNI TGGCCA 2 cut(s) 1716, 2030
MlyI GAGTC 3 cut(s) 479, 541, 685
MmeI TCCRAC 3 cut(s) 764, 1827, 2978
Mox20I TGGCCA 2 cut(s) 1716, 2030
Mph1103I ATGCAT 2 cut(s) 1552, 2901
MroI TCCGGA 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 1782
MscI TGGCCA 2 cut(s) 1716, 2030
MseI TTAA 4 cut(s) 210, 434, 1572, 2520
MslI CAYNNNNRTG 2 cut(s) 1482, 2445
Msp20I TGGCCA 2 cut(s) 1716, 2030
MspA1I CMGCKG 2 cut(s) 2358, 2692
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 5 cut(s) 15, 69, 224, 1183, 1490
MspR9I CCNGG 9 cut(s) 325, 849, 933, 1184, 1254, 1491, 1590, 2145, 2779
MunI CAATTG 1 cut(s) 2562
Mva1269I GAATGC 3 cut(s) 683, 813, 2603
MvaI CCWGG 7 cut(s) 325, 849, 933, 1254, 1590, 2145, 2779
MvnI CGCG 2 cut(s) 53, 145
MwoI GCNNNNNNNGC 7 cut(s) 88, 552, 706, 1193, 1534, 2108, 2597
NciI CCSGG 2 cut(s) 1184, 1491
NcoI CCATGG 2 cut(s) 502, 1096
NdeI CATATG 2 cut(s) 2293, 2814
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 7 cut(s) 104, 370, 445, 764, 1164, 2130, 2787
NmeAIII GCCGAG 2 cut(s) 64, 2236
NmuCI GTSAC 5 cut(s) 271, 1831, 1937, 2171, 2818
NsiI ATGCAT 2 cut(s) 1552, 2901
NspI RCATGY 3 cut(s) 1071, 1208, 1487
PaeR7I CTCGAG 1 cut(s) 536
PagI TCATGA 3 cut(s) 400, 1360, 1750
PciI ACATGT 2 cut(s) 1067, 1204
PcsI WCGNNNNNNNCGW 1 cut(s) 2575
PctI GAATGC 3 cut(s) 683, 813, 2603
PdmI GAANNNNTTC 1 cut(s) 1782
PfeI GAWTC 7 cut(s) 838, 1124, 1147, 1645, 1658, 2078, 2162
PflFI GACNNNGTC 1 cut(s) 1829
PfoI TCCNGGA 2 cut(s) 1588, 2143
PleI GAGTC 3 cut(s) 478, 540, 684
PpsI GAGTC 3 cut(s) 478, 540, 684
PpuMI RGGWCCY 1 cut(s) 232
PscI ACATGT 2 cut(s) 1067, 1204
PshBI ATTAAT 1 cut(s) 1572
PsiI TTATAA 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 7 cut(s) 323, 847, 931, 1252, 1588, 2143, 2777
PspFI CCCAGC 1 cut(s) 1331
PspGI CCWGG 7 cut(s) 323, 847, 931, 1252, 1588, 2143, 2777
PspN4I GGNNCC 7 cut(s) 104, 370, 445, 764, 1164, 2130, 2787
PspPI GGNCC 4 cut(s) 232, 1250, 1379, 2480
PspPPI RGGWCCY 1 cut(s) 232
PsrI GAACNNNNNNTAC 2 cut(s) 1220, 1252
PstNI CAGNNNCTG 1 cut(s) 978
PsuI RGATCY 4 cut(s) 2268, 2525, 2683, 3036
PsyI GACNNNGTC 1 cut(s) 1829
PvuII CAGCTG 1 cut(s) 2358
RsaI GTAC 5 cut(s) 215, 764, 786, 2074, 2640
RsaNI GTAC 5 cut(s) 214, 763, 785, 2073, 2639
RseI CAYNNNNRTG 2 cut(s) 1482, 2445
SaqAI TTAA 4 cut(s) 210, 434, 1572, 2520
Sau96I GGNCC 4 cut(s) 232, 1250, 1379, 2480
ScaI AGTACT 1 cut(s) 2074
SchI GAGTC 3 cut(s) 479, 541, 685
ScrFI CCNGG 9 cut(s) 325, 849, 933, 1184, 1254, 1491, 1590, 2145, 2779
SduI GDGCHC 3 cut(s) 711, 1539, 3005
SfaNI GCATC 8 cut(s) 22, 409, 601, 709, 1537, 1559, 2446, 2587
SfcI CTRYAG 1 cut(s) 1404
Sfr274I CTCGAG 1 cut(s) 536
SinI GGWCC 3 cut(s) 232, 1250, 2480
SlaI CTCGAG 1 cut(s) 536
SmiMI CAYNNNNRTG 2 cut(s) 1482, 2445
SmlI CTYRAG 2 cut(s) 209, 536
SmoI CTYRAG 2 cut(s) 209, 536
SsiI CCGC 6 cut(s) 89, 143, 200, 203, 830, 2692
SspMI CTAG 5 cut(s) 36, 696, 1836, 1866, 2261
StyD4I CCNGG 9 cut(s) 323, 847, 931, 1182, 1252, 1489, 1588, 2143, 2777
StyI CCWWGG 4 cut(s) 502, 695, 1096, 2229
TaiI ACGT 5 cut(s) 1038, 1305, 1356, 1392, 2944
TaqI TCGA 4 cut(s) 537, 963, 1023, 1423
TaqII GACCGA 1 cut(s) 1380
TatI WGTACW 2 cut(s) 2072, 2638
TauI GCSGC 4 cut(s) 91, 203, 832, 2695
TfiI GAWTC 7 cut(s) 838, 1124, 1147, 1645, 1658, 2078, 2162
Tru1I TTAA 4 cut(s) 210, 434, 1572, 2520
Tru9I TTAA 4 cut(s) 210, 434, 1572, 2520
TscAI CASTG 4 cut(s) 979, 992, 1476, 2139
TseFI GTSAC 5 cut(s) 271, 1831, 1937, 2171, 2818
TseI GCWGC 7 cut(s) 460, 1631, 1918, 2111, 2600, 3062, 3065
Tsp45I GTSAC 5 cut(s) 271, 1831, 1937, 2171, 2818
TspGWI ACGGA 3 cut(s) 660, 1464, 1701
TspRI CASTG 4 cut(s) 979, 992, 1476, 2139
Tth111I GACNNNGTC 1 cut(s) 1829
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 3 cut(s) 232, 1250, 2480
VspI ATTAAT 1 cut(s) 1572
XagI CCTNNNNNAGG 2 cut(s) 612, 1869
XapI RAATTY 6 cut(s) 7, 449, 1261, 1962, 2510, 2949
XbaI TCTAGA 1 cut(s) 2260
XceI RCATGY 3 cut(s) 1071, 1208, 1487
XcmI CCANNNNNNNNNTGG 1 cut(s) 1328
XhoI CTCGAG 1 cut(s) 536
XmaJI CCTAGG 1 cut(s) 695
XmnI GAANNNNTTC 1 cut(s) 1782
XspI CTAG 5 cut(s) 36, 696, 1836, 1866, 2261
ZrmI AGTACT 1 cut(s) 2074
Zsp2I ATGCAT 2 cut(s) 1552, 2901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.