Rorug04G0041300

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005070
Physical Location & Seq
Forward (+)
6429582 .. 6430250
669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0041300.1

Sequence Viewer

Length: 669 bp
ATGCAGAGGAACACTGGCTTCTGCAAAAGCTTGGTGAAAGACCACATTACTACCATGACAAGGGCCACTGATGGAGATTCCAACAACAAGAAGATGAAGAATATTGAAGATGATGAAGACTATATGAATGTGTATCCATGGCCTCCGAGGTCTTACACATGTAGCTTCTGCAAGAGAGAATTCAAATCTGCTCAAGCTCTGGGTGGCCATATGAATGTTCATAGGAAAGACCGAGCCAGGCTCAGAAGCTCACCCCCAACCGATTTTATTGGTCAGTACACTCACAGTACTATACTTAATCTCAACCTCAAACCTAACCCTAACCCTAATTTCGCATCCTCACCACTACCATCATCATCTCCCTTCTCTTCTGTTTCAACTCGGCAGCTCCCACCGTTAACTACTAGCTCTTCGATATCACAACCTTCTCATCCCCCTCCTACATTTCCATCACCATTTGATACTTGCCATAGTGAAAACATGAAATGGGCTGTGGGTGGTACCCTTTCCACTCCTTTAAACCTCAAACCTTTGGATTTAAGTACTCAAATGAAGATTAATACAGAGTCTCAAGAAGATGGATGCATGAAATTGGACTTGGAAATTGGTGTTCTTGGCTGTAACTCAAGGGATGACATCGATTTGGAGCTTCGATTAGGCTACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

222

Amino Acids

24.74

Weight (kDa)

7.64

Isoelectric Point (pI)

60.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 52 - 76 4.5e-07 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 500
AccB1I GGYRCC 1 cut(s) 500
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 4 cut(s) 278, 289, 502, 544
AfiI CCNNNNNNNGG 1 cut(s) 60
AflIII ACRYGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 107, 184, 378
AjnI CCWGG 1 cut(s) 236
AjuI GAANNNNNNNTTGG 2 cut(s) 581, 613
AluBI AGCT 7 cut(s) 30, 165, 197, 249, 388, 408, 649
AluI AGCT 7 cut(s) 30, 165, 197, 249, 388, 408, 649
Alw26I GTCTC 1 cut(s) 573
AoxI GGCC 3 cut(s) 63, 140, 205
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 1 cut(s) 179
AseI ATTAAT 1 cut(s) 558
Asp718I GGTACC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 4 cut(s) 46, 243, 333, 444
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 123
BbvI GCAGC 1 cut(s) 397
BccI CCATC 4 cut(s) 65, 358, 457, 572
BciT130I CCWGG 1 cut(s) 238
BciVI GTATCC 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 573
BfaI CTAG 2 cut(s) 405, 667
BfuI GTATCC 1 cut(s) 144
BisI GCNGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 387
BmcAI AGTACT 2 cut(s) 289, 544
Bme1390I CCNGG 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 344, 572
BpiI GAAGAC 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 225, 257
BpuEI CTTGAG 3 cut(s) 177, 555, 610
Bsa29I ATCGAT 1 cut(s) 639
BsaJI CCNNGG 2 cut(s) 137, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 19
BseBI CCWGG 1 cut(s) 238
BseCI ATCGAT 1 cut(s) 639
BseDI CCNNGG 2 cut(s) 137, 146
BseGI GGATG 4 cut(s) 335, 430, 587, 637
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 256
BseNI ACTGG 1 cut(s) 19
BseXI GCAGC 1 cut(s) 397
BshFI GGCC 3 cut(s) 65, 142, 207
BshNI GGYRCC 1 cut(s) 500
BshVI ATCGAT 1 cut(s) 639
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 573
BsnI GGCC 3 cut(s) 65, 142, 207
Bsp19I CCATGG 1 cut(s) 137
BspANI GGCC 3 cut(s) 65, 142, 207
BspCNI CTCAG 1 cut(s) 255
BspDI ATCGAT 1 cut(s) 639
BspLI GGNNCC 1 cut(s) 502
BspQI GCTCTTC 1 cut(s) 415
BspT107I GGYRCC 1 cut(s) 500
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 2 cut(s) 137, 146
BssT1I CCWWGG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 287, 396
Bst6I CTCTTC 2 cut(s) 373, 415
BstDEI CTNAG 1 cut(s) 242
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 4 cut(s) 335, 430, 587, 637
BstMAI GTCTC 1 cut(s) 573
BstNI CCWGG 1 cut(s) 238
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 1 cut(s) 123
Bsu15I ATCGAT 1 cut(s) 639
BsuI GTATCC 1 cut(s) 144
BsuRI GGCC 3 cut(s) 65, 142, 207
BsuTUI ATCGAT 1 cut(s) 639
BtgI CCRYGG 1 cut(s) 137
BtsCI GGATG 4 cut(s) 335, 430, 587, 637
BtsIMutI CAGTG 2 cut(s) 12, 66
Cfr13I GGNCC 1 cut(s) 63
ClaI ATCGAT 1 cut(s) 639
Csp6I GTAC 4 cut(s) 277, 288, 501, 543
CviAII CATG 5 cut(s) 55, 138, 159, 481, 586
CviQI GTAC 4 cut(s) 277, 288, 501, 543
DdeI CTNAG 1 cut(s) 242
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 373, 415
EarI CTCTTC 2 cut(s) 373, 415
Eco130I CCWWGG 1 cut(s) 137
Eco32I GATATC 1 cut(s) 417
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 236
EcoRV GATATC 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 5 cut(s) 58, 141, 162, 484, 589
FatI CATG 5 cut(s) 54, 137, 158, 480, 585
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 1 cut(s) 386
FokI GGATG 4 cut(s) 322, 417, 594, 644
Fsp4HI GCNGC 1 cut(s) 386
FspBI CTAG 2 cut(s) 405, 667
GluI GCNGC 1 cut(s) 386
HaeIII GGCC 3 cut(s) 65, 142, 207
Hin1II CATG 5 cut(s) 58, 141, 162, 484, 589
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 2 cut(s) 77, 566
HpaI GTTAAC 1 cut(s) 399
HphI GGTGA 4 cut(s) 46, 243, 333, 444
Hpy166II GTNNAC 2 cut(s) 279, 399
Hpy188I TCNGA 2 cut(s) 147, 245
Hpy188III TCNNGA 1 cut(s) 572
Hpy8I GTNNAC 2 cut(s) 279, 399
HpyAV CCTTC 2 cut(s) 373, 435
HpyCH4III ACNGT 2 cut(s) 287, 396
HpyCH4V TGCA 4 cut(s) 4, 24, 171, 585
HpyF3I CTNAG 1 cut(s) 242
Hsp92II CATG 5 cut(s) 58, 141, 162, 484, 589
KpnI GGTACC 1 cut(s) 504
KspAI GTTAAC 1 cut(s) 399
LguI GCTCTTC 1 cut(s) 415
LmnI GCTCC 2 cut(s) 393, 646
LpnPI CCDG 3 cut(s) 185, 223, 250
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 2 cut(s) 344, 572
MaeI CTAG 2 cut(s) 405, 667
MaeIII GTNAC 1 cut(s) 620
MboII GAAGA 8 cut(s) 103, 109, 119, 128, 360, 402, 565, 587
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 4 cut(s) 179, 328, 590, 603
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 6 cut(s) 141, 153, 317, 349, 447, 533
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 587
MscI TGGCCA 1 cut(s) 207
MseI TTAA 5 cut(s) 297, 398, 518, 539, 558
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 238
MvaI CCWGG 1 cut(s) 238
NcoI CCATGG 1 cut(s) 137
NdeI CATATG 1 cut(s) 210
NlaIII CATG 5 cut(s) 58, 141, 162, 484, 589
NlaIV GGNNCC 1 cut(s) 502
NmeAIII GCCGAG 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 587
NspI RCATGY 1 cut(s) 162
PciI ACATGT 1 cut(s) 158
PciSI GCTCTTC 1 cut(s) 415
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 387
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PscI ACATGT 1 cut(s) 158
PshBI ATTAAT 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 1 cut(s) 63
RsaI GTAC 4 cut(s) 278, 289, 502, 544
RsaNI GTAC 4 cut(s) 277, 288, 501, 543
RseI CAYNNNNRTG 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 415
SaqAI TTAA 5 cut(s) 297, 398, 518, 539, 558
SatI GCNGC 1 cut(s) 386
Sau96I GGNCC 1 cut(s) 63
ScaI AGTACT 2 cut(s) 289, 544
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 238
SfaNI GCATC 2 cut(s) 344, 572
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 3 cut(s) 192, 570, 625
SmoI CTYRAG 3 cut(s) 192, 570, 625
Sse9I AATT 4 cut(s) 179, 328, 590, 603
SspI AATATT 1 cut(s) 103
SspMI CTAG 2 cut(s) 405, 667
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 137
TaaI ACNGT 2 cut(s) 287, 396
TaqI TCGA 3 cut(s) 413, 639, 652
TaqII GACCGA 1 cut(s) 246
TasI AATT 4 cut(s) 179, 328, 590, 603
TatI WGTACW 3 cut(s) 276, 287, 542
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 5 cut(s) 297, 398, 518, 539, 558
Tru9I TTAA 5 cut(s) 297, 398, 518, 539, 558
TscAI CASTG 2 cut(s) 19, 73
TseI GCWGC 1 cut(s) 385
TspDTI ATGAA 8 cut(s) 110, 129, 140, 209, 227, 497, 566, 602
TspRI CASTG 2 cut(s) 19, 73
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 1 cut(s) 179
XceI RCATGY 1 cut(s) 162
XspI CTAG 2 cut(s) 405, 667
ZrmI AGTACT 2 cut(s) 289, 544
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.