Prupe.1G089600_v2.0.a1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005070
Physical Location & Seq
Forward (+)
6706535 .. 6713264
6730 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G089600.1

Sequence Viewer

Length: 3339 bp
ATGTGTAATAAAGGAATTGCGTGCTTGAGCGAGTCTGAGAACCCAATTGATCAGCAACACCCTCATCAAGCTGTGTACTTGATGGATAGCCCTTCCGCTACCCCTCACTTTGGTTCTAATTCCACCGCTGTTTCGAGCCCTAATTGGAATGAGGAGAGCCCACGTGTTAAATTTCTATGTAGCTTTTCGGGTAGTATATTGCCTCGCCCACAAGATGGGAAGCTCCGGTATGTGGGTGGAGAGACACGAATTGTGAGTGTTCCACGAGATATTAAGTATGAGGAGTTGATGAACAAGATGAGGGACCTTTATGAAGGTGCAGCAGTGCTCAAGTACCAGCAACCTGATGAAGATCTTGACGCACTTGTATCGGTTGTGAACGATGATGATGTGACTAATATGATGGAAGAGTATGATAAGCTGGGCTCCGGTGATGGTTTCACTAGGCTCAGGATTTTTCTGTTTTCGCATCCGGAACAAGATGGGTCATCACACTATGAGGGAGATGAAAGGGATAATGAGAGGAGGTATGTCGATGCTCTCAATAATTTAAATGATGGTTCCGACTTTAGGAAGCAGCATCCTGAGTCCCCTTTTATTAATCCAGTTGATGATATCCATATAGCTGAACAGTTTTTTAGCCCAATAAGTCTTGAGGGTGGCCTTCAGAGAAGTTGTGATATGTCCGCACCTCAATATAACTTGCATCACCTCAAGATACCTCATATAGGATCAGGGCAACACCATCAACCGATTACTCAGAGGTATAACGAAATGGAAGCTCCTTGGAGTCCTGCATACTATTCTCCTAGGCATCATGGGCATCTTGATCCAAGACCAATGCCAGAATTTCCATCTTCTCCTTCCTCTGCACGGTACCGTATTCCATTTCCAGATTTACCTGATAAATGTTTAGATAGAATGCCTGAAGAATATGCACGACAGCCATTGAATCATCAGCCTGCATATGAACACCAAACACAATATACTGAGAATGTTGTGTGGCTTCCAAGTGGAGCTATATCTGGTGAAAAGTCTGGCTTTCCTGGTCACATCTTTCATGGAACCAATGTTCTCGAAGGAAACAGTATTTGTGAGCATTGCCGGATGAATTTCCAGAGAAACCAACCACATTTTGAGCAATCCAACACGGTGAATGGATTTCACCAGGTTGCTAACCCAAGCACTGAGTGTCCTCCGAATAGAGAAAGTTTCATGATGAATTCAGATGCAAAGTTGCACCATGAGATATACGCAAGTGAACAGAATAATGGTCCTCCATCTCTTTATAATGAGACTCCAAATCATGAAAGAGGATGGATTCCACATCATCATTTGAATTGTCGTACTGAGGAAGCAAGACCACATGTCTCTGGAGCTGGGAAATTGAATGATCACTACATTGTAGATGGTCCCAGCATGAATTTGCCTCTTGGTCCTAGTAACATGGTTGATGGCCATCATGTCTCTTCCAATTATGTTCATCAGCGAGTTGGACCTGAAATTGGAAATGAAGTGTTTCATGACCGACTTGTGCCTGCTCCACCCCATGTACACGTTGCTCCCTCAGAAGAGCGTGGGGTTCGTTATGGGAATCCTCCTTATGCCTTTGGAGGAGATAATCCTTACCCAGTGTCTCATGGACATGTACCTGGACCTGCAGTATGGAGAAATGTTCAGAGCCCAATGCATGCTGCGCCCTCTTACGAAGCGTCCAATTCTGCTCCACAAGTGAATGGCACTGTTAACCCAGGATTTCTCAGGCATGAGGATAGTCCAAGGTTTGGCCTTACAGTAGACAATCAAAATATTTGGGCTGACTCCTCGCAGCAAATGTTAGGTTTTGATGGAAAAGTTGTTCCAGACTATTCGTATGGCCACACTTTGAAGTTTAATCCAAACACACTTGGTCAGGAAAATCATCCTCCATTTCCTTCAGACCCTACTCAACCCACACCTGATATGCTAAATTGTGCTATTCCTTTAGACCCCGTTACTGGAGTTGTGAGGTTGGAGGGAGAAAGTTTGCCTGGAGAAGAGAAGGAGGTAAATCTTGTGGAGAAGCTAGAGTACTCTGACATGCAGGGTATAAGTCAGAACAAATTTTCCGATAAAAATTATGAAATGGTATCTCCTGAGTTGATACATTCAAATTTCCCTAAGCTTACTGAAGTAAGTGGTGATGTTGTCAAAACTAGTGACAATGATCATTCTACTCCTGAAGTTCCAAAGCTTTCTGTTAGCCATTTCATACCTGAGTTGATGGCTTCTGTAAAAAGAGCTGCATTAGAGGAGGCTGAGGAGGTGAAAGCCAATGTTAAAGAAAGTGGAGATCCTGAGAAGGATAGTTCAATAGCCGAAGAAGCAGCTGCAAACAATTTGGAACGAGTGAACACTCCTGGAGATGGGGAATTGGATTCTGATAACGATTATCTGAACAATTCCAAAATTGAGCCAACGAAGGCTGAGGCAGAAGCTATTTCCAAAGGATTGCAGACAATAAAGAATGATGATCTAGAGGAGATTCGAGAATTGGGTTCTGGAACTTATGGGGCTGTTTTTCATGGGAAGTGGAAGGGGTCTGACGTAGCGATAAAGAGAATAAAATCCAGCTGTTTTGCTGGGAGACCATCAGAAAGAGAACGCTTGATTGCAGATTTCTGGAAGGAGGCTTTGATACTTGGTTCGTTGCATCACCCAAATGTTGTTTCTTTCTATGGTATTGTGCGTGACGGTCCTGATGGGTCTTTAGCAACAGTGACCGAATTCATGGTTAATGGATCTTTGAAACAGTTTTTGCAGAAAAAAGACAGAACAATTGATCGTCGTAAAAGATTGATCATTGCTATGGATGCTGCATTTGGAATGGAGTATCTGCACGGGAGAAATATAGTACATTTTGACTTAAAGTGTGAAAATCTTTTAGTAAATATGAGAGATCCACAGCGGCCTGTATGTAAGATTGGTGATTTGGGATTATCAAAAGTAAAACAACACACTTTAGTGTCTGGAGGTGTTCGTGGAACTTTGCCTTGGATGGCGCCTGAACTTCTTAGTGGGAAAAGTAACATGGTAACAGAAAAGATTGATGTTTACTCGTTTGGAATTGTTATGTGGGAACTGCTTACGGGGGATGAGCCATATACGGATATGCATTGTGCTTCTATAATTGGAGGAATTGTGAACAACACATTGCGTCCTCAAATTCCCACTTGGTGTGATCCTGAATGGAAATCTTTGATGGAAAGTTGTTGGGCCTCTGAGCCTTCTCAGAGGCCATCATTTTCTGAAATCTCTCAGAAGCTAAGGAATATGGCTGCTGCAATGAATGTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1113

Amino Acids

124.29

Weight (kDa)

5.42

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1290
Acc36I ACCTGC 1 cut(s) 1666
Acc65I GGTACC 1 cut(s) 876
AccB1I GGYRCC 2 cut(s) 876, 3040
AccB7I CCANNNNNTGG 2 cut(s) 215, 1784
AccI GTMKAC 1 cut(s) 1797
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 4 cut(s) 96, 126, 687, 2947
AclWI GGATC 6 cut(s) 739, 824, 2327, 2788, 2933, 3215
AcoI YGGCCR 2 cut(s) 1456, 1876
AcsI RAATTY 9 cut(s) 170, 848, 1111, 1222, 1423, 2102, 2152, 2765, 3204
AcuI CTGAAG 5 cut(s) 650, 948, 1920, 2190, 2241
AcvI CACGTG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 3041
AdeI CACNNNGTG 2 cut(s) 1191, 3216
AfaI GTAC 8 cut(s) 77, 335, 878, 1348, 1554, 1650, 2072, 2895
AflIII ACRYGT 4 cut(s) 163, 1366, 1555, 1645
AgsI TTSAA 7 cut(s) 952, 1339, 1390, 1888, 2151, 2352, 2788
AhdI GACNNNNNGTC 1 cut(s) 1367
AhlI ACTAGT 1 cut(s) 2195
AjnI CCWGG 6 cut(s) 1045, 1167, 1651, 1750, 2029, 2398
AjuI GAANNNNNNNTTGG 4 cut(s) 2692, 2724, 3016, 3048
AleI CACNNNNGTG 1 cut(s) 3002
AloI GAACNNNNNNTCC 6 cut(s) 1056, 1088, 1842, 1874, 2090, 2122
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 6 cut(s) 236, 1289, 1375, 1471, 1641, 2620
AlwI GGATC 6 cut(s) 739, 824, 2327, 2788, 2933, 3215
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 7 cut(s) 661, 1456, 1786, 1876, 2948, 3255, 3275
ApoI RAATTY 9 cut(s) 170, 848, 1111, 1222, 1423, 2102, 2152, 2765, 3204
AseI ATTAAT 1 cut(s) 600
Asp700I GAANNNNTTC 1 cut(s) 1518
Asp718I GGTACC 1 cut(s) 876
AspA2I CCTAGG 1 cut(s) 809
AspLEI GCGC 2 cut(s) 1699, 3043
AspS9I GGNCC 8 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735, 3255
AsuHPI GGTGA 9 cut(s) 443, 701, 1040, 1157, 1165, 2192, 2317, 2687, 2978
AvaII GGWCC 7 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735
AvrII CCTAGG 1 cut(s) 809
BalI TGGCCA 2 cut(s) 1458, 1878
BanI GGYRCC 2 cut(s) 876, 3040
BanII GRGCYC 4 cut(s) 140, 161, 428, 1685
BarI GAAGNNNNNNTAC 2 cut(s) 2054, 2086
BauI CACGAG 1 cut(s) 264
BbrPI CACGTG 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 2 cut(s) 2298, 2466
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
BclI TGATCA 4 cut(s) 49, 1393, 2206, 2838
BcoDI GTCTC 6 cut(s) 236, 1289, 1375, 1471, 1641, 2620
BcuI ACTAGT 1 cut(s) 2195
BfaI CTAG 6 cut(s) 444, 810, 1440, 2066, 2196, 2516
BfmI CTRYAG 1 cut(s) 1659
BfoI RGCGCY 1 cut(s) 3044
BfuAI ACCTGC 1 cut(s) 1666
BglII AGATCT 1 cut(s) 352
BlnI CCTAGG 1 cut(s) 809
BmcAI AGTACT 1 cut(s) 2072
Bme1390I CCNGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
Bme18I GGWCC 7 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735
BmeRI GACNNNNNGTC 1 cut(s) 1367
BmgT120I GGNCC 8 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735, 3255
BmiI GGNNCC 7 cut(s) 305, 427, 562, 878, 1066, 1414, 3042
BmrFI CCNGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
BmrI ACTGGG 1 cut(s) 1625
BmsI GCATC 9 cut(s) 478, 526, 589, 715, 823, 832, 1219, 2701, 2842
BmuI ACTGGG 1 cut(s) 1625
BpmI CTGGAG 5 cut(s) 1395, 2019, 2052, 2421, 3030
Bpu10I CCTNAGC 5 cut(s) 449, 2160, 2298, 2466, 3305
BpuEI CTTGAG 4 cut(s) 46, 314, 674, 698
BsaAI YACGTR 1 cut(s) 164
BsaBI GATNNNNATC 2 cut(s) 351, 1458
BsaHI GRCGYC 1 cut(s) 3041
BsaI GGTCTC 1 cut(s) 2620
BsaJI CCNNGG 5 cut(s) 785, 809, 1750, 1778, 3032
BsaWI WCCGGW 3 cut(s) 225, 428, 472
BsaXI ACNNNNNCTCC 2 cut(s) 3165, 3195
Bse1I ACTGG 3 cut(s) 605, 1631, 2002
Bse3DI GCAATG 4 cut(s) 1099, 2841, 3191, 3330
Bse8I GATNNNNATC 2 cut(s) 351, 1458
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
BseDI CCNNGG 5 cut(s) 785, 809, 1750, 1778, 3032
BseGI GGATG 8 cut(s) 469, 580, 1113, 1322, 1921, 2857, 3042, 3139
BseJI GATNNNNATC 2 cut(s) 351, 1458
BseMI GCAATG 4 cut(s) 1099, 2841, 3191, 3330
BseNI ACTGG 3 cut(s) 605, 1631, 2002
BseRI GAGGAG 8 cut(s) 167, 296, 538, 1629, 1813, 2306, 2315, 2534
BseYI CCCAGC 4 cut(s) 421, 1379, 1415, 2621
BsgI GTGCAG 3 cut(s) 339, 855, 2861
BshFI GGCC 7 cut(s) 663, 1458, 1788, 1878, 2950, 3257, 3277
BshNI GGYRCC 2 cut(s) 876, 3040
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 4 cut(s) 226, 429, 473, 1105
BslFI GGGAC 3 cut(s) 317, 574, 1398
BsmAI GTCTC 6 cut(s) 236, 1289, 1375, 1471, 1641, 2620
BsmFI GGGAC 3 cut(s) 317, 574, 1398
BsmI GAATGC 1 cut(s) 927
BsnI GGCC 7 cut(s) 663, 1458, 1788, 1878, 2950, 3257, 3277
Bso31I GGTCTC 1 cut(s) 2620
Bsp1286I GDGCHC 5 cut(s) 140, 161, 330, 428, 1685
Bsp13I TCCGGA 1 cut(s) 472
Bsp1407I TGTACA 1 cut(s) 1552
BspACI CCGC 4 cut(s) 96, 126, 687, 2947
BspANI GGCC 7 cut(s) 663, 1458, 1788, 1878, 2950, 3257, 3277
BspEI TCCGGA 1 cut(s) 472
BspHI TCATGA 3 cut(s) 1215, 1306, 1522
BspLI GGNNCC 7 cut(s) 305, 427, 562, 878, 1066, 1414, 3042
BspMAI CTGCAG 1 cut(s) 1663
BspMI ACCTGC 1 cut(s) 1666
BspPI GGATC 6 cut(s) 739, 824, 2327, 2788, 2933, 3215
BspQI GCTCTTC 1 cut(s) 1566
BspT107I GGYRCC 2 cut(s) 876, 3040
BspTNI GGTCTC 1 cut(s) 2620
BsrDI GCAATG 4 cut(s) 1099, 2841, 3191, 3330
BsrGI TGTACA 1 cut(s) 1552
BsrI ACTGG 3 cut(s) 605, 1631, 2002
BssECI CCNNGG 5 cut(s) 785, 809, 1750, 1778, 3032
BssNI GRCGYC 1 cut(s) 3041
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 4 cut(s) 785, 809, 1778, 3032
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
Bst6I CTCTTC 4 cut(s) 402, 1474, 1566, 2031
BstACI GRCGYC 1 cut(s) 3041
BstAUI TGTACA 1 cut(s) 1552
BstBAI YACGTR 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 22, 963, 1539, 1692
BstENI CCTNNNNNAGG 1 cut(s) 726
BstF5I GGATG 8 cut(s) 469, 580, 1113, 1322, 1921, 2857, 3042, 3139
BstH2I RGCGCY 1 cut(s) 3044
BstHHI GCGC 2 cut(s) 1699, 3043
BstMAI GTCTC 6 cut(s) 236, 1289, 1375, 1471, 1641, 2620
BstMWI GCNNNNNNNGC 4 cut(s) 820, 1696, 2363, 2852
BstNI CCWGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
BstNSI RCATGY 4 cut(s) 1370, 1649, 1694, 2083
BstSCI CCNGG 6 cut(s) 1045, 1167, 1651, 1750, 2029, 2398
BstSFI CTRYAG 1 cut(s) 1659
BstX2I RGATCY 4 cut(s) 352, 2332, 2780, 2938
BstYI RGATCY 4 cut(s) 352, 2332, 2780, 2938
BsuRI GGCC 7 cut(s) 663, 1458, 1788, 1878, 2950, 3257, 3277
BtsCI GGATG 8 cut(s) 469, 580, 1113, 1322, 1921, 2857, 3042, 3139
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 5 cut(s) 330, 1185, 1638, 1740, 2763
BveI ACCTGC 1 cut(s) 1666
Cac8I GCNNGC 4 cut(s) 22, 963, 1539, 1692
CciI TCATGA 3 cut(s) 1215, 1306, 1522
CfoI GCGC 2 cut(s) 1699, 3043
Cfr13I GGNCC 8 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735, 3255
CseI GACGC 3 cut(s) 368, 1701, 3185
CsiI ACCWGGT 1 cut(s) 1167
Csp6I GTAC 8 cut(s) 76, 334, 877, 1347, 1553, 1649, 2071, 2894
CviQI GTAC 8 cut(s) 76, 334, 877, 1347, 1553, 1649, 2071, 2894
DinI GGCGCC 1 cut(s) 3042
DraI TTTAAA 1 cut(s) 552
DraIII CACNNNGTG 2 cut(s) 1191, 3216
DriI GACNNNNNGTC 1 cut(s) 1367
EaeI YGGCCR 2 cut(s) 1456, 1876
Eam1104I CTCTTC 4 cut(s) 402, 1474, 1566, 2031
Eam1105I GACNNNNNGTC 1 cut(s) 1367
EarI CTCTTC 4 cut(s) 402, 1474, 1566, 2031
Eco130I CCWWGG 4 cut(s) 785, 809, 1778, 3032
Eco24I GRGCYC 4 cut(s) 140, 161, 428, 1685
Eco31I GGTCTC 1 cut(s) 2620
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 7 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735
Eco57I CTGAAG 5 cut(s) 650, 948, 1920, 2190, 2241
Eco72I CACGTG 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 726
EcoO109I RGGNCCY 1 cut(s) 304
EcoRI GAATTC 2 cut(s) 1222, 2765
EcoRII CCWGG 6 cut(s) 1045, 1167, 1651, 1750, 2029, 2398
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 4 cut(s) 785, 809, 1778, 3032
EcoT22I ATGCAT 2 cut(s) 1692, 3156
EcoT38I GRGCYC 4 cut(s) 140, 161, 428, 1685
EgeI GGCGCC 1 cut(s) 3042
EheI GGCGCC 1 cut(s) 3042
ErhI CCWWGG 4 cut(s) 785, 809, 1778, 3032
FalI AAGNNNNNCTT 2 cut(s) 1025, 1057
FaqI GGGAC 3 cut(s) 317, 574, 1398
FauNDI CATATG 1 cut(s) 967
FbaI TGATCA 4 cut(s) 49, 1393, 2206, 2838
FblI GTMKAC 1 cut(s) 1797
FokI GGATG 8 cut(s) 456, 567, 1120, 1329, 1908, 2864, 3049, 3146
FriOI GRGCYC 4 cut(s) 140, 161, 428, 1685
FspBI CTAG 6 cut(s) 444, 810, 1440, 2066, 2196, 2516
GlaI GCGC 2 cut(s) 1698, 3042
GsaI CCCAGC 4 cut(s) 425, 1383, 1419, 2625
GsuI CTGGAG 5 cut(s) 1395, 2019, 2052, 2421, 3030
HaeII RGCGCY 1 cut(s) 3044
HaeIII GGCC 7 cut(s) 663, 1458, 1788, 1878, 2950, 3257, 3277
HapII CCGG 4 cut(s) 226, 429, 473, 1105
HgaI GACGC 3 cut(s) 368, 1701, 3185
HhaI GCGC 2 cut(s) 1699, 3043
Hin1I GRCGYC 1 cut(s) 3041
Hin6I GCGC 2 cut(s) 1697, 3041
HinP1I GCGC 2 cut(s) 1697, 3041
HincII GTYRAC 1 cut(s) 1747
HindII GTYRAC 1 cut(s) 1747
HindIII AAGCTT 2 cut(s) 2162, 2231
HpaI GTTAAC 1 cut(s) 1747
HpaII CCGG 4 cut(s) 226, 429, 473, 1105
HphI GGTGA 9 cut(s) 443, 701, 1040, 1157, 1165, 2192, 2317, 2687, 2978
Hpy166II GTNNAC 9 cut(s) 76, 379, 1262, 1555, 1747, 1798, 2392, 3094, 3184
Hpy8I GTNNAC 9 cut(s) 76, 379, 1262, 1555, 1747, 1798, 2392, 3094, 3184
Hpy99I CGWCG 1 cut(s) 2829
HpyCH4IV ACGT 3 cut(s) 163, 1557, 2586
HpyF10VI GCNNNNNNNGC 4 cut(s) 820, 1696, 2363, 2852
HpySE526I ACGT 3 cut(s) 163, 1557, 2586
Hsp92I GRCGYC 1 cut(s) 3041
HspAI GCGC 2 cut(s) 1697, 3041
KasI GGCGCC 1 cut(s) 3040
Kpn2I TCCGGA 1 cut(s) 472
KpnI GGTACC 1 cut(s) 880
Ksp22I TGATCA 4 cut(s) 49, 1393, 2206, 2838
KspAI GTTAAC 1 cut(s) 1747
LguI GCTCTTC 1 cut(s) 1566
LmnI GCTCC 8 cut(s) 228, 431, 787, 1016, 1376, 1546, 1567, 1729
LweI GCATC 9 cut(s) 478, 526, 589, 715, 823, 832, 1219, 2701, 2842
MabI ACCWGGT 1 cut(s) 1167
MaeI CTAG 6 cut(s) 444, 810, 1440, 2066, 2196, 2516
MaeII ACGT 3 cut(s) 163, 1557, 2586
MaeIII GTNAC 9 cut(s) 391, 1049, 1442, 1993, 2198, 2729, 2758, 3065, 3073
MboII GAAGA 8 cut(s) 362, 419, 849, 941, 1461, 1583, 2048, 2372
MfeI CAATTG 2 cut(s) 45, 2817
MflI RGATCY 4 cut(s) 352, 2332, 2780, 2938
MhlI GDGCHC 5 cut(s) 140, 161, 330, 428, 1685
MlsI TGGCCA 2 cut(s) 1458, 1878
MluNI TGGCCA 2 cut(s) 1458, 1878
Mly113I GGCGCC 1 cut(s) 3041
MlyI GAGTC 5 cut(s) 41, 596, 799, 1291, 1814
MmeI TCCRAC 4 cut(s) 588, 1170, 1474, 1992
Mox20I TGGCCA 2 cut(s) 1458, 1878
Mph1103I ATGCAT 2 cut(s) 1692, 3156
MroI TCCGGA 1 cut(s) 472
MroXI GAANNNNTTC 1 cut(s) 1518
MscI TGGCCA 2 cut(s) 1458, 1878
MseI TTAA 9 cut(s) 168, 273, 551, 600, 1746, 1893, 2319, 2775, 2906
MslI CAYNNNNRTG 4 cut(s) 1644, 2700, 2846, 3002
Msp20I TGGCCA 2 cut(s) 1458, 1878
MspA1I CMGCKG 4 cut(s) 128, 2369, 2613, 2947
MspI CCGG 4 cut(s) 226, 429, 473, 1105
MspR9I CCNGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
MunI CAATTG 2 cut(s) 45, 2817
Mva1269I GAATGC 1 cut(s) 927
MvaI CCWGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
MwoI GCNNNNNNNGC 4 cut(s) 820, 1696, 2363, 2852
NarI GGCGCC 1 cut(s) 3041
NdeI CATATG 1 cut(s) 967
NlaIV GGNNCC 7 cut(s) 305, 427, 562, 878, 1066, 1414, 3042
NmuCI GTSAC 5 cut(s) 391, 1049, 2198, 2729, 2758
NsiI ATGCAT 2 cut(s) 1692, 3156
NspI RCATGY 4 cut(s) 1370, 1649, 1694, 2083
OliI CACNNNNGTG 1 cut(s) 3002
PaeI GCATGC 1 cut(s) 1694
PagI TCATGA 3 cut(s) 1215, 1306, 1522
PciI ACATGT 2 cut(s) 1366, 1645
PciSI GCTCTTC 1 cut(s) 1566
PctI GAATGC 1 cut(s) 927
PdmI GAANNNNTTC 1 cut(s) 1518
PfeI GAWTC 5 cut(s) 952, 1321, 1594, 2417, 2524
PflMI CCANNNNNTGG 2 cut(s) 215, 1784
PfoI TCCNGGA 1 cut(s) 2398
PleI GAGTC 5 cut(s) 40, 595, 798, 1291, 1814
PluTI GGCGCC 1 cut(s) 3044
PmaCI CACGTG 1 cut(s) 164
PmlI CACGTG 1 cut(s) 164
PpsI GAGTC 5 cut(s) 40, 595, 798, 1291, 1814
Ppu21I YACGTR 1 cut(s) 164
PpuMI RGGWCCY 1 cut(s) 304
PscI ACATGT 2 cut(s) 1366, 1645
PshBI ATTAAT 1 cut(s) 600
PsiI TTATAA 1 cut(s) 1290
Psp5II RGGWCCY 1 cut(s) 304
Psp6I CCWGG 6 cut(s) 1045, 1167, 1651, 1750, 2029, 2398
PspCI CACGTG 1 cut(s) 164
PspFI CCCAGC 4 cut(s) 421, 1379, 1415, 2621
PspGI CCWGG 6 cut(s) 1045, 1167, 1651, 1750, 2029, 2398
PspN4I GGNNCC 7 cut(s) 305, 427, 562, 878, 1066, 1414, 3042
PspPI GGNCC 8 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735, 3255
PspPPI RGGWCCY 1 cut(s) 304
PstI CTGCAG 1 cut(s) 1663
PsuI RGATCY 4 cut(s) 352, 2332, 2780, 2938
PvuII CAGCTG 2 cut(s) 2369, 2613
RsaI GTAC 8 cut(s) 77, 335, 878, 1348, 1554, 1650, 2072, 2895
RsaNI GTAC 8 cut(s) 76, 334, 877, 1347, 1553, 1649, 2071, 2894
RseI CAYNNNNRTG 4 cut(s) 1644, 2700, 2846, 3002
SapI GCTCTTC 1 cut(s) 1566
SaqAI TTAA 9 cut(s) 168, 273, 551, 600, 1746, 1893, 2319, 2775, 2906
Sau96I GGNCC 8 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735, 3255
ScaI AGTACT 1 cut(s) 2072
SchI GAGTC 5 cut(s) 41, 596, 799, 1291, 1814
ScrFI CCNGG 6 cut(s) 1047, 1169, 1653, 1752, 2031, 2400
SduI GDGCHC 5 cut(s) 140, 161, 330, 428, 1685
SexAI ACCWGGT 1 cut(s) 1167
SfaNI GCATC 9 cut(s) 478, 526, 589, 715, 823, 832, 1219, 2701, 2842
SfcI CTRYAG 1 cut(s) 1659
SfoI GGCGCC 1 cut(s) 3042
SinI GGWCC 7 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735
SmiI ATTTAAAT 1 cut(s) 552
SmiMI CAYNNNNRTG 4 cut(s) 1644, 2700, 2846, 3002
SmlI CTYRAG 4 cut(s) 25, 329, 653, 713
SmoI CTYRAG 4 cut(s) 25, 329, 653, 713
SpeI ACTAGT 1 cut(s) 2195
SphI GCATGC 1 cut(s) 1694
SsiI CCGC 4 cut(s) 96, 126, 687, 2947
SspDI GGCGCC 1 cut(s) 3040
SspI AATATT 1 cut(s) 1810
SspMI CTAG 6 cut(s) 444, 810, 1440, 2066, 2196, 2516
StyD4I CCNGG 6 cut(s) 1045, 1167, 1651, 1750, 2029, 2398
StyI CCWWGG 4 cut(s) 785, 809, 1778, 3032
SwaI ATTTAAAT 1 cut(s) 552
TaiI ACGT 3 cut(s) 166, 1560, 2589
TaqI TCGA 4 cut(s) 134, 534, 1077, 2527
TaqII GACCGA 2 cut(s) 1542, 2777
TatI WGTACW 4 cut(s) 75, 1552, 2070, 2893
TauI GCSGC 1 cut(s) 2950
TfiI GAWTC 5 cut(s) 952, 1321, 1594, 2417, 2524
Tru1I TTAA 9 cut(s) 168, 273, 551, 600, 1746, 1893, 2319, 2775, 2906
Tru9I TTAA 9 cut(s) 168, 273, 551, 600, 1746, 1893, 2319, 2775, 2906
TscAI CASTG 5 cut(s) 330, 1192, 1638, 1747, 2763
TseFI GTSAC 5 cut(s) 391, 1049, 2198, 2729, 2758
Tsp45I GTSAC 5 cut(s) 391, 1049, 2198, 2729, 2758
TspGWI ACGGA 1 cut(s) 3161
TspRI CASTG 5 cut(s) 330, 1192, 1638, 1747, 2763
Van91I CCANNNNNTGG 2 cut(s) 215, 1784
VpaK11BI GGWCC 7 cut(s) 304, 1274, 1412, 1436, 1496, 1655, 2735
VspI ATTAAT 1 cut(s) 600
XagI CCTNNNNNAGG 1 cut(s) 726
XapI RAATTY 9 cut(s) 170, 848, 1111, 1222, 1423, 2102, 2152, 2765, 3204
XbaI TCTAGA 1 cut(s) 2515
XceI RCATGY 4 cut(s) 1370, 1649, 1694, 2083
XmaJI CCTAGG 1 cut(s) 809
XmiI GTMKAC 1 cut(s) 1797
XmnI GAANNNNTTC 1 cut(s) 1518
XspI CTAG 6 cut(s) 444, 810, 1440, 2066, 2196, 2516
ZrmI AGTACT 1 cut(s) 2072
Zsp2I ATGCAT 2 cut(s) 1692, 3156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.