Rorug04G0041200

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005070
Physical Location & Seq
Forward (+)
6421117 .. 6423236
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0041200.1

Sequence Viewer

Length: 936 bp
ATGGCCAATGGTTTCGATTTCGGTTGGTGCTACTGTCCTACTGATGTTGATGGTTCTTGCATTGCTGAAAGTCAAGACACTGGGTCTTCGGCAACCTCATCAAGATTTGCAGTTGGCCAGTTGTCTACTTATCTGGCCTCAGGCCTGCAATTTCAATATCATCAACAGGTGGACTGCATTGTGTTCGACAAGACAGGGACTCTTACAATTGGGAAGCCACTGGTTGTTAACACACGACTCATGAAAAATATAGTGCTTCGAGAATTCTATGAACTTGTTGCTGCAGCTGAGGTTAACAGTGAACACCCCTTGGCCAAGGCCATTGTTGAGTATGCCAAAAAATTCAGAGAAGATGAAGAGAATCCAGCCGGGCCAGAAGCGCATGACTTTGCCTCCATTACTGGTCACGGGGTGAAGGCTATTGTTCAGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTGCAGTTCCACTCGATGCAGAAGACTACCTAGCAGAAGCTGAAGGGCTGGCTCAAACTGGGATTCTAGTAGCCATAGATGGAGAAGTGGCTGGAGTTCTAGCAATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTAGAAGCATAATGGTGACAGGTGACAATTGGGGAATTGCAAATTCTATTGCCAATGAAGTTGGTATTGATACTGTTATAGCCGAAGCGAAACCTGATCACCAGAAAGCAGAGAAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGCGATGGTGGGAGATGGCATCAATGACTCACCAGCACTTGTGGCGGCAGATGTAGGAATGGCAATTGGTGCTGGCACTAACATTGCAATTGAGGCAGCAGACATTGTGCTAATGAAGAGTAGGTTTCCTCTTTATTTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

311

Amino Acids

33.02

Weight (kDa)

4.55

Isoelectric Point (pI)

24.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 57 - 281 7.6e-33 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 125
AciI CCGC 1 cut(s) 836
AclWI GGATC 1 cut(s) 584
AcoI YGGCCR 3 cut(s) 3, 115, 312
AcsI RAATTY 3 cut(s) 263, 341, 685
AcuI CTGAAG 2 cut(s) 540, 762
AdeI CACNNNGTG 1 cut(s) 412
AgsI TTSAA 1 cut(s) 155
AhdI GACNNNNNGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 3 cut(s) 287, 459, 518
AluI AGCT 3 cut(s) 287, 459, 518
Alw21I GWGCWC 1 cut(s) 610
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 7 cut(s) 3, 115, 135, 142, 312, 318, 371
ApeKI GCWGC 3 cut(s) 281, 284, 887
ApoI RAATTY 3 cut(s) 263, 341, 685
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 2 cut(s) 371, 469
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 5 cut(s) 424, 670, 677, 734, 813
AvaII GGWCC 1 cut(s) 469
AxyI CCTNAGG 1 cut(s) 139
BalI TGGCCA 3 cut(s) 5, 117, 314
BbsI GAAGAC 2 cut(s) 78, 507
Bbv12I GWGCWC 1 cut(s) 610
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 3 cut(s) 268, 296, 899
BccI CCATC 5 cut(s) 44, 457, 551, 790, 800
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 603
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 1 cut(s) 370
BfaI CTAG 3 cut(s) 509, 545, 578
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 4 cut(s) 282, 285, 837, 888
BlsI GCNGC 4 cut(s) 283, 286, 838, 889
Bme1390I CCNGG 2 cut(s) 370, 603
Bme18I GGWCC 1 cut(s) 469
BmeRI GACNNNNNGTC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 371, 469
BmrFI CCNGG 2 cut(s) 370, 603
BmrI ACTGGG 2 cut(s) 90, 546
BmsI GCATC 2 cut(s) 484, 819
BmuI ACTGGG 2 cut(s) 90, 546
BpiI GAAGAC 2 cut(s) 78, 507
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 594, 614
BpuMI CCSGG 1 cut(s) 370
BsaJI CCNNGG 2 cut(s) 309, 315
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bse1I ACTGG 5 cut(s) 85, 118, 225, 406, 541
Bse21I CCTNAGG 1 cut(s) 139
Bse3DI GCAATG 3 cut(s) 60, 477, 873
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 2 cut(s) 309, 315
BseMI GCAATG 3 cut(s) 60, 477, 873
BseMII CTCAG 2 cut(s) 153, 279
BseNI ACTGG 5 cut(s) 85, 118, 225, 406, 541
BseXI GCAGC 3 cut(s) 268, 296, 899
BshFI GGCC 7 cut(s) 5, 117, 137, 144, 314, 320, 373
BsiHKAI GWGCWC 1 cut(s) 610
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 7 cut(s) 5, 117, 137, 144, 314, 320, 373
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 2 cut(s) 589, 739
BspACI CCGC 1 cut(s) 836
BspANI GGCC 7 cut(s) 5, 117, 137, 144, 314, 320, 373
BspCNI CTCAG 2 cut(s) 152, 280
BspHI TCATGA 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 1 cut(s) 584
BsrDI GCAATG 3 cut(s) 60, 477, 873
BsrI ACTGG 5 cut(s) 85, 118, 225, 406, 541
BssECI CCNNGG 2 cut(s) 309, 315
BssMI GATC 2 cut(s) 589, 739
BssT1I CCWWGG 2 cut(s) 309, 315
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 4 cut(s) 35, 299, 718, 790
Bst6I CTCTTC 2 cut(s) 351, 902
BstAPI GCANNNNNTGC 1 cut(s) 860
BstC8I GCNNGC 4 cut(s) 146, 528, 774, 865
BstDEI CTNAG 2 cut(s) 139, 288
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 2 cut(s) 592, 742
BstMBI GATC 2 cut(s) 589, 739
BstMWI GCNNNNNNNGC 4 cut(s) 379, 833, 860, 884
BstNI CCWGG 1 cut(s) 603
BstSCI CCNGG 2 cut(s) 368, 601
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 268, 296, 899
BstV2I GAAGAC 2 cut(s) 78, 507
Bsu36I CCTNAGG 1 cut(s) 139
BsuRI GGCC 7 cut(s) 5, 117, 137, 144, 314, 320, 373
BtgZI GCGATG 1 cut(s) 809
BtsIMutI CAGTG 5 cut(s) 78, 218, 304, 593, 786
Cac8I GCNNGC 4 cut(s) 146, 528, 774, 865
CaiI CAGNNNCTG 1 cut(s) 518
CciI TCATGA 1 cut(s) 240
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 2 cut(s) 371, 469
CsiI ACCWGGT 1 cut(s) 601
CviAII CATG 2 cut(s) 241, 383
DdeI CTNAG 2 cut(s) 139, 288
DpnI GATC 2 cut(s) 591, 741
DpnII GATC 2 cut(s) 589, 739
DraIII CACNNNGTG 1 cut(s) 412
DriI GACNNNNNGTC 1 cut(s) 82
EaeI YGGCCR 3 cut(s) 3, 115, 312
Eam1104I CTCTTC 2 cut(s) 351, 902
Eam1105I GACNNNNNGTC 1 cut(s) 82
EarI CTCTTC 2 cut(s) 351, 902
Eco130I CCWWGG 2 cut(s) 309, 315
Eco147I AGGCCT 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 469
Eco57I CTGAAG 2 cut(s) 540, 762
Eco81I CCTNAGG 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 2 cut(s) 309, 315
ErhI CCWWGG 2 cut(s) 309, 315
FaeI CATG 2 cut(s) 244, 386
FaiI YATR 9 cut(s) 242, 251, 270, 333, 384, 554, 626, 653, 722
FaqI GGGAC 1 cut(s) 211
FatI CATG 2 cut(s) 240, 382
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 125
Fnu4HI GCNGC 4 cut(s) 282, 285, 837, 888
Fsp4HI GCNGC 4 cut(s) 282, 285, 837, 888
FspBI CTAG 3 cut(s) 509, 545, 578
GlaI GCGC 1 cut(s) 381
GluI GCNGC 4 cut(s) 282, 285, 837, 888
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 7 cut(s) 5, 117, 137, 144, 314, 320, 373
HapII CCGG 1 cut(s) 369
HhaI GCGC 1 cut(s) 382
Hin1II CATG 2 cut(s) 244, 386
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 2 cut(s) 229, 295
HindII GTYRAC 2 cut(s) 229, 295
HinfI GANTC 5 cut(s) 199, 237, 361, 541, 818
HpaI GTTAAC 2 cut(s) 229, 295
HpaII CCGG 1 cut(s) 369
HphI GGTGA 5 cut(s) 424, 670, 677, 734, 813
Hpy166II GTNNAC 6 cut(s) 126, 172, 229, 295, 302, 469
Hpy188I TCNGA 2 cut(s) 347, 589
Hpy188III TCNNGA 5 cut(s) 74, 102, 241, 260, 611
Hpy8I GTNNAC 6 cut(s) 126, 172, 229, 295, 302, 469
HpyAV CCTTC 3 cut(s) 409, 515, 757
HpyCH4III ACNGT 4 cut(s) 35, 299, 718, 790
HpyF10VI GCNNNNNNNGC 4 cut(s) 379, 833, 860, 884
HpyF3I CTNAG 2 cut(s) 139, 288
Hsp92II CATG 2 cut(s) 244, 386
HspAI GCGC 1 cut(s) 380
Ksp22I TGATCA 1 cut(s) 739
KspAI GTTAAC 2 cut(s) 229, 295
Kzo9I GATC 2 cut(s) 589, 739
Lsp1109I GCAGC 3 cut(s) 268, 296, 899
LweI GCATC 2 cut(s) 484, 819
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 3 cut(s) 509, 545, 578
MaeIII GTNAC 3 cut(s) 404, 658, 665
MalI GATC 2 cut(s) 591, 741
MboI GATC 2 cut(s) 589, 739
MboII GAAGA 5 cut(s) 78, 362, 368, 512, 919
MfeI CAATTG 4 cut(s) 207, 670, 855, 879
MhlI GDGCHC 1 cut(s) 610
MlsI TGGCCA 3 cut(s) 5, 117, 314
MluNI TGGCCA 3 cut(s) 5, 117, 314
MlyI GAGTC 3 cut(s) 193, 231, 812
MnlI CCTC 6 cut(s) 106, 148, 283, 403, 877, 930
Mox20I TGGCCA 3 cut(s) 5, 117, 314
MscI TGGCCA 3 cut(s) 5, 117, 314
MseI TTAA 2 cut(s) 228, 294
MslI CAYNNNNRTG 1 cut(s) 656
Msp20I TGGCCA 3 cut(s) 5, 117, 314
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 2 cut(s) 370, 603
MunI CAATTG 4 cut(s) 207, 670, 855, 879
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 4 cut(s) 379, 833, 860, 884
NciI CCSGG 1 cut(s) 370
NdeII GATC 2 cut(s) 589, 739
NlaIII CATG 2 cut(s) 244, 386
NmuCI GTSAC 3 cut(s) 404, 658, 665
PagI TCATGA 1 cut(s) 240
PceI AGGCCT 1 cut(s) 144
PfeI GAWTC 2 cut(s) 361, 541
PkrI GCNGC 4 cut(s) 283, 286, 838, 889
PleI GAGTC 3 cut(s) 193, 231, 812
PpsI GAGTC 3 cut(s) 193, 231, 812
Psp6I CCWGG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 601
PspPI GGNCC 2 cut(s) 371, 469
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 287
RseI CAYNNNNRTG 1 cut(s) 656
SaqAI TTAA 2 cut(s) 228, 294
SatI GCNGC 4 cut(s) 282, 285, 837, 888
Sau3AI GATC 2 cut(s) 589, 739
Sau96I GGNCC 2 cut(s) 371, 469
SchI GAGTC 3 cut(s) 193, 231, 812
ScrFI CCNGG 2 cut(s) 370, 603
SduI GDGCHC 1 cut(s) 610
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 2 cut(s) 484, 819
SfcI CTRYAG 1 cut(s) 282
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 2 cut(s) 609, 629
SmoI CTYRAG 2 cut(s) 609, 629
SseBI AGGCCT 1 cut(s) 144
SsiI CCGC 1 cut(s) 836
SspMI CTAG 3 cut(s) 509, 545, 578
StuI AGGCCT 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 368, 601
StyI CCWWGG 2 cut(s) 309, 315
TaaI ACNGT 4 cut(s) 35, 299, 718, 790
TaqI TCGA 4 cut(s) 15, 186, 259, 492
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 2 cut(s) 361, 541
Tru1I TTAA 2 cut(s) 228, 294
Tru9I TTAA 2 cut(s) 228, 294
TscAI CASTG 5 cut(s) 85, 225, 304, 600, 793
TseFI GTSAC 3 cut(s) 404, 658, 665
TseI GCWGC 3 cut(s) 281, 284, 887
Tsp45I GTSAC 3 cut(s) 404, 658, 665
TspDTI ATGAA 6 cut(s) 257, 285, 369, 653, 714, 920
TspRI CASTG 5 cut(s) 85, 225, 304, 600, 793
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 3 cut(s) 263, 341, 685
XmiI GTMKAC 1 cut(s) 125
XspI CTAG 3 cut(s) 509, 545, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.