RchiOBHm_Chr4g0404461

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005070
Physical Location & Seq
Forward (+)
24242862 .. 24244037
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37600

Sequence Viewer

Length: 915 bp
ATGTGTAATAAAGGAATTGCATGCTTGAGTGAGTCGGATAACCCCATTGATCAGCAGCAGCAACAGGCTGTCTACTTGATGGAGAGCCCTTCGACTACCCCTCACTTTGGCTCTAATTCCACCCATGTTTCCAGCCCTTTTTTGAATGAGGACAATCCCCCTCGTGTTAAATTTCTGTGTAGTTTCTCCGGTAGTATATTGCCTCGGCCGCAGGATGGGAGGCTCCGATATGTCGGTGGGGAGACACGGATTGTTAGTGTTCCGCGAGACATTAGGTATGAGGAGTTGATGAGCAAGATGAGGGAGCTTTATGAAGGGGCGGCGGTGCTGAAGTACCAGCAACCGGATGAGGACCTTGACGCCCTTGTCTCGGTTGTGAATGATGATGATGTGACCAACATGATGGAAGAGTATGATAAGTTGGGGTCGGGTGATGGTTTCACCAGGCTCAGGATTTTTTTGTTTTCGTATCCCGACCAAGATGGTGGCTCCTCACACTATGACGGGGATGAAGGGGATCATGAGAGGAGGTATGTTGATGCTCTCAATAACTTAAATGATGGTTCCGAATTTAGGAAGCAGCATTTAGAGTCCCCTGTTATCACTTCAGTTGATGATCTCCATGGAGTTGAACAGTTCTTTAGTCCAATGAGTCTCGAGGGTGGCCTCCATAGCCAGAGAAACGAGATATCCATGTCTCATTATAACCTGCATCGCCTCAAAATACCTCATATAGGTTCAGGGCAACACCATCAGCCCATTAGCCAAAGGTTTAACGACATGGAAGCTCCGTGGAGTCCTGCATACTATTCTCCTAGGCATCATGGGCACCTTGATCCAAGACCAATGGCAGAGTTTCCAACTTCTCCTACCTCTGCACGGTACCGTATGCCATTTGCAGATGAATGCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

304

Amino Acids

34.54

Weight (kDa)

5.11

Isoelectric Point (pI)

58.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 72 - 155 1.7e-18 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AasI GACNNNNNNGTC 1 cut(s) 365
Acc36I ACCTGC 1 cut(s) 717
Acc65I GGTACC 1 cut(s) 882
AccB1I GGYRCC 2 cut(s) 828, 882
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 265
AciI CCGC 4 cut(s) 209, 263, 320, 323
AclWI GGATC 2 cut(s) 525, 830
AcoI YGGCCR 1 cut(s) 206
AcsI RAATTY 2 cut(s) 170, 569
AcuI CTGAAG 2 cut(s) 350, 591
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 2 cut(s) 335, 884
AfiI CCNNNNNNNGG 8 cut(s) 107, 215, 343, 370, 450, 573, 734, 879
AgsI TTSAA 2 cut(s) 145, 632
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 2 cut(s) 307, 788
AluI AGCT 2 cut(s) 307, 788
Alw26I GTCTC 5 cut(s) 236, 261, 373, 659, 702
AlwI GGATC 2 cut(s) 525, 830
Ama87I CYCGRG 1 cut(s) 656
AoxI GGCC 2 cut(s) 206, 664
ApeKI GCWGC 3 cut(s) 55, 58, 580
ApoI RAATTY 2 cut(s) 170, 569
Asp718I GGTACC 1 cut(s) 882
AspA2I CCTAGG 1 cut(s) 815
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 433, 443
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 1 cut(s) 352
AvrII CCTAGG 1 cut(s) 815
BaeGI GKGCMC 1 cut(s) 831
BanI GGYRCC 2 cut(s) 828, 882
BanII GRGCYC 1 cut(s) 89
BauI CACGAG 1 cut(s) 162
BbvI GCAGC 3 cut(s) 67, 70, 592
BccI CCATC 7 cut(s) 73, 209, 397, 428, 476, 554, 759
BciT130I CCWGG 1 cut(s) 445
BciVI GTATCC 1 cut(s) 480
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 5 cut(s) 236, 261, 373, 659, 702
BfaI CTAG 1 cut(s) 816
BfuAI ACCTGC 1 cut(s) 717
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 5 cut(s) 56, 59, 209, 321, 581
BlnI CCTAGG 1 cut(s) 815
BlsI GCNGC 5 cut(s) 57, 60, 210, 322, 582
Bme1390I CCNGG 1 cut(s) 445
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 5 cut(s) 224, 490, 565, 830, 884
BmrFI CCNGG 1 cut(s) 445
BmsI GCATC 3 cut(s) 529, 721, 829
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 360
BsaJI CCNNGG 4 cut(s) 203, 622, 791, 815
BsaWI WCCGGW 2 cut(s) 188, 343
Bsc4I CCNNNNNNNGG 8 cut(s) 107, 215, 343, 370, 450, 573, 734, 879
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 4 cut(s) 203, 622, 791, 815
BseGI GGATG 3 cut(s) 220, 352, 514
BseLI CCNNNNNNNGG 8 cut(s) 107, 215, 343, 370, 450, 573, 734, 879
BseMII CTCAG 1 cut(s) 463
BseRI GAGGAG 3 cut(s) 296, 481, 541
BseSI GKGCMC 1 cut(s) 831
BseX3I CGGCCG 1 cut(s) 206
BseXI GCAGC 3 cut(s) 67, 70, 592
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 2 cut(s) 208, 666
BshNI GGYRCC 2 cut(s) 828, 882
BsiEI CGRYCG 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 2 cut(s) 189, 344
BslFI GGGAC 1 cut(s) 577
BslI CCNNNNNNNGG 8 cut(s) 107, 215, 343, 370, 450, 573, 734, 879
BsmAI GTCTC 5 cut(s) 236, 261, 373, 659, 702
BsmFI GGGAC 1 cut(s) 577
BsmI GAATGC 1 cut(s) 911
BsnI GGCC 2 cut(s) 208, 666
BsoBI CYCGRG 1 cut(s) 656
Bsp1286I GDGCHC 2 cut(s) 89, 831
Bsp143I GATC 4 cut(s) 49, 517, 616, 835
Bsp19I CCATGG 1 cut(s) 622
BspACI CCGC 4 cut(s) 209, 263, 320, 323
BspANI GGCC 2 cut(s) 208, 666
BspCNI CTCAG 1 cut(s) 462
BspFNI CGCG 1 cut(s) 265
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 5 cut(s) 224, 490, 565, 830, 884
BspMI ACCTGC 1 cut(s) 717
BspPI GGATC 2 cut(s) 525, 830
BspT107I GGYRCC 2 cut(s) 828, 882
BssECI CCNNGG 4 cut(s) 203, 622, 791, 815
BssMI GATC 4 cut(s) 49, 517, 616, 835
BssNI GRCGYC 1 cut(s) 360
BssSI CACGAG 1 cut(s) 162
BssT1I CCWWGG 2 cut(s) 622, 815
Bst2BI CACGAG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 3 cut(s) 636, 882, 887
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 1 cut(s) 449
BstDSI CCRYGG 2 cut(s) 622, 791
BstENI CCTNNNNNAGG 1 cut(s) 732
BstF5I GGATG 3 cut(s) 220, 352, 514
BstFNI CGCG 1 cut(s) 265
BstKTI GATC 4 cut(s) 52, 520, 619, 838
BstMAI GTCTC 5 cut(s) 236, 261, 373, 659, 702
BstMBI GATC 4 cut(s) 49, 517, 616, 835
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 208, 672, 826
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 2 cut(s) 24, 913
BstSCI CCNGG 1 cut(s) 443
BstSLI GKGCMC 1 cut(s) 831
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 3 cut(s) 67, 70, 592
BstXI CCANNNNNNTGG 2 cut(s) 403, 485
BstZI CGGCCG 1 cut(s) 206
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 2 cut(s) 208, 666
BtgI CCRYGG 2 cut(s) 622, 791
BtgZI GCGATG 1 cut(s) 698
BtsCI GGATG 3 cut(s) 220, 352, 514
BveI ACCTGC 1 cut(s) 717
Cac8I GCNNGC 1 cut(s) 22
CciI TCATGA 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 352
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 334, 883
CviAII CATG 9 cut(s) 21, 125, 400, 521, 623, 694, 781, 824, 910
CviQI GTAC 2 cut(s) 334, 883
DdeI CTNAG 1 cut(s) 449
DpnI GATC 4 cut(s) 51, 519, 618, 837
DpnII GATC 4 cut(s) 49, 517, 616, 835
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 206
EagI CGGCCG 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EclXI CGGCCG 1 cut(s) 206
Eco130I CCWWGG 2 cut(s) 622, 815
Eco24I GRGCYC 1 cut(s) 89
Eco32I GATATC 1 cut(s) 690
Eco47I GGWCC 1 cut(s) 352
Eco52I CGGCCG 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 350, 591
Eco88I CYCGRG 1 cut(s) 656
EcoNI CCTNNNNNAGG 1 cut(s) 732
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 443
EcoRV GATATC 1 cut(s) 690
EcoT14I CCWWGG 2 cut(s) 622, 815
EcoT22I ATGCAT 1 cut(s) 911
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 2 cut(s) 622, 815
FaeI CATG 9 cut(s) 24, 128, 403, 524, 626, 697, 784, 827, 913
FaqI GGGAC 1 cut(s) 577
FatI CATG 9 cut(s) 20, 124, 399, 520, 622, 693, 780, 823, 909
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 56, 59, 209, 321, 581
FokI GGATG 3 cut(s) 227, 359, 521
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 5 cut(s) 56, 59, 209, 321, 581
FspBI CTAG 1 cut(s) 816
GluI GCNGC 5 cut(s) 56, 59, 209, 321, 581
HaeIII GGCC 2 cut(s) 208, 666
HapII CCGG 2 cut(s) 189, 344
HgaI GACGC 1 cut(s) 368
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 9 cut(s) 24, 128, 403, 524, 626, 697, 784, 827, 913
HinfI GANTC 4 cut(s) 32, 590, 652, 796
HpaII CCGG 2 cut(s) 189, 344
HphI GGTGA 2 cut(s) 433, 443
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 37, 227, 568
Hpy188III TCNNGA 4 cut(s) 451, 473, 521, 656
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 99, 308, 506
HpyCH4III ACNGT 3 cut(s) 636, 882, 887
HpyCH4V TGCA 6 cut(s) 20, 712, 803, 878, 899, 909
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 672, 826
HpyF3I CTNAG 1 cut(s) 449
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 9 cut(s) 24, 128, 403, 524, 626, 697, 784, 827, 913
KpnI GGTACC 1 cut(s) 886
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 4 cut(s) 49, 517, 616, 835
LmnI GCTCC 4 cut(s) 228, 304, 494, 793
Lsp1109I GCAGC 3 cut(s) 67, 70, 592
LweI GCATC 3 cut(s) 529, 721, 829
MaeI CTAG 1 cut(s) 816
MaeIII GTNAC 1 cut(s) 391
MalI GATC 4 cut(s) 51, 519, 618, 837
MboI GATC 4 cut(s) 49, 517, 616, 835
MboII GAAGA 1 cut(s) 419
MhlI GDGCHC 2 cut(s) 89, 831
MluCI AATT 4 cut(s) 15, 115, 170, 569
MlyI GAGTC 4 cut(s) 41, 599, 661, 805
MmeI TCCRAC 2 cut(s) 15, 884
Mph1103I ATGCAT 1 cut(s) 911
MseI TTAA 3 cut(s) 168, 554, 774
MspI CCGG 2 cut(s) 189, 344
MspR9I CCNGG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 911
MvaI CCWGG 1 cut(s) 445
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 3 cut(s) 208, 672, 826
NcoI CCATGG 1 cut(s) 622
NdeII GATC 4 cut(s) 49, 517, 616, 835
NlaIII CATG 9 cut(s) 24, 128, 403, 524, 626, 697, 784, 827, 913
NlaIV GGNNCC 5 cut(s) 224, 490, 565, 830, 884
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 391
NsiI ATGCAT 1 cut(s) 911
NspI RCATGY 2 cut(s) 24, 913
PaeI GCATGC 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 656
PagI TCATGA 1 cut(s) 520
PctI GAATGC 1 cut(s) 911
PkrI GCNGC 5 cut(s) 57, 60, 210, 322, 582
PleI GAGTC 4 cut(s) 40, 598, 660, 804
PpsI GAGTC 4 cut(s) 40, 598, 660, 804
PpuMI RGGWCCY 1 cut(s) 352
PsiI TTATAA 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 5 cut(s) 224, 490, 565, 830, 884
PspPI GGNCC 1 cut(s) 352
PspPPI RGGWCCY 1 cut(s) 352
RsaI GTAC 2 cut(s) 335, 884
RsaNI GTAC 2 cut(s) 334, 883
SaqAI TTAA 3 cut(s) 168, 554, 774
SatI GCNGC 5 cut(s) 56, 59, 209, 321, 581
Sau3AI GATC 4 cut(s) 49, 517, 616, 835
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 4 cut(s) 41, 599, 661, 805
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 2 cut(s) 89, 831
SfaNI GCATC 3 cut(s) 529, 721, 829
Sfr274I CTCGAG 1 cut(s) 656
SinI GGWCC 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 656
SmlI CTYRAG 2 cut(s) 25, 656
SmoI CTYRAG 2 cut(s) 25, 656
SphI GCATGC 1 cut(s) 24
Sse9I AATT 4 cut(s) 15, 115, 170, 569
SsiI CCGC 4 cut(s) 209, 263, 320, 323
SspMI CTAG 1 cut(s) 816
StyD4I CCNGG 1 cut(s) 443
StyI CCWWGG 2 cut(s) 622, 815
TaaI ACNGT 3 cut(s) 636, 882, 887
TaqI TCGA 2 cut(s) 92, 657
TasI AATT 4 cut(s) 15, 115, 170, 569
TauI GCSGC 2 cut(s) 211, 323
Tru1I TTAA 3 cut(s) 168, 554, 774
Tru9I TTAA 3 cut(s) 168, 554, 774
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 3 cut(s) 55, 58, 580
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 327, 525
TspGWI ACGGA 2 cut(s) 262, 780
VpaK11BI GGWCC 1 cut(s) 352
XagI CCTNNNNNAGG 1 cut(s) 732
XapI RAATTY 2 cut(s) 170, 569
XceI RCATGY 2 cut(s) 24, 913
XhoI CTCGAG 1 cut(s) 656
XmaJI CCTAGG 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 1 cut(s) 816
Zsp2I ATGCAT 1 cut(s) 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.