MD16G1266900.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005070
Physical Location & Seq
Reverse (-)
34059930 .. 34067869
7940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1266900.v1.1.491

Sequence Viewer

Length: 3321 bp
ATGTGTAACAAAGGAATTGAGTGCTTAAGTGACTCCGAGAACCCAATTAATCATCAGGCTGTGTACTTGATGGATAGCCCTTCCGCTACCCCCCACTTTGGTTCTAATTCCACTGCTGCTCTGTCCCCCAATTTGAATGAGGAGAATCCGCGTGTTAAGTTTCTGTGTAGTTTTTCGGGTAGTATATTGCCCCGCCCACAAGATGGGAAACTCCGGTATGTGGGTGGAGAGACGCGAATTGTGAGTGTTCCACAAGATATCAAGTTCGAGGAGTTGATGAACAAGATACGGGAGCTTTATGAAGGGGCAGCAGTGCTCAAGTACCAGCAACCTGATGAGGACCTTGATGCTCTTGTCTCGGTTGTGAACGATGATGATGTGACTAACATGATGGAAGAGTATGATAAGTTGGGATCTGGCGATGGTTTCACTAGGCTCAGGATTTTTTTGTTTTCGCATCCTGACCAGGATAGTTCATCGCACTATGAGGGTGATGAAAGGGATAATGAGAGGAGGTATGTCGACGCTCTCAATACTTTAAATGATGGCTCCGAATTTAGGAAGCAGTATCCTGAGTCCCCTCTTATTAATCCTGTTGATGATCTCCATATAGCTGAACAATTTTTTAGTCCAATAAGTCTTGAGGGTTGCCTTCATAGCCAGAGAAATTGTGATATATCCATGGCTCAATACAACTTGCATCAGCTCAAGATACCTCATGCAGGATCGGGGCAACACCATCAACCAATTAGTCAGAGGTATAACGAAATGGAAGCTCCTTGGAGTCCTGCATACTATTCTCCTGGGCATCATGGGCACATTGATTCAAGACCAATGGCAGAGTTTCCTTCCTCTCCTTCCTCTGCATGGTACCGTATGCCGTTTCCAGATGTAGCCGATAAATGTTTAGATAGACTGCCTGAAGAATATGCACGACAGCCATTGAATCATCAGCCTCCATATGAACACCTTTCTGAGAATGTTTCATTGCTTCCAAGTGGAGCTATAGGTGGTGAAAAGTCAGGATTTCCGGGTAACGTTTTTCATGGAAACAATGCTGTTGAAGGAAACAGTATTTGTGAACACTGCCGGATGGCTTTCCAGAGAAACCAACCACATTTTGAGCAACCAAACATGGCAAATGGATTTCAGCAGGTTCCTAATCCAAGCAGCGAGGGTACTCCAAATAGAGAGACTTTCATGATGAATCCAGATGCAAAGTTGCACCATGAAATATACGCAAGTGAACAGAATAATGGTCCTCCTCATTTTAATGAGACTCCAAATCATGAAAGAGGGTGGGCTCAACATCATCATTTGAATTGTCGGACCGAGGAAACAAGACCACATGCTTCTGGAGCTGGGAAATTGAATGATCCTTACATTGTAGATGGTCCCAACATGAATTTGCCTCTTGGTCCTAATATGGTGGATGGCCATTACATCTCTTCCAATTATGTTCATCACCGAGCTGGACCTAAAGTGGGAAATGAAGTGTTTCACGAACGATCTGTGGCTGCTCTACCCAATGTACACATTGCTCCTGTAGAAGAGCGTGGTGTTCGTTATGGAAATCTTCCTTATGCCTATGGAGGCGATAATATTTATCTGGGGTCTCATGGACATGCACCTGGACATGCAGTATGGAGAAATGTTCAGATCCCAATGCATGCTGCGCCTCCTCATGAAGCATCCGTTTCTGCTCCACAAGTGAATGGTTCTGTTAATGTGGGATATTTCAGACGTGAGGATAGTCCAAGGTTTTCCATTGCATTAGACAATCAAAATATTTGGGTTGACTCCTCGCAGAAAATGTTAGGTTTGGAAGGGAAAGCTGTTCCTCCAGACTATTCTTATGGGCACGCTGTAAAGTTGAACCCAAACGCACTTTGTCAAGAAAATCATCAAGCATTTCCTCTAGAACCCATTCAGTCCACACCTGATATGATAAATTGTGCTATTCCTTTGAACCCCGTCGCCGGAGTTGTAAGGTTGGAGGAAAAGAGTTCACCTGGAGTAAATGAGGTGAATATTGTGGACAAGGTGGAGAACTCTGAAACGGAGGTTATAAACCCAAACAATCATTGTGATAAAAGTGGTGGTGTGGTATCTCTTGAGTCGATAAATTCAAATTTCAGTAAGCTTGCAGAAGAAAGTGGTAATGCTGGCAAAACAAGTGACGAACATCAGTCTACTCGTGAACTTTTAAAGCTTTCTGTTAATGATTTGAGTTTCATACCGGAGTTGATTGCTTCTGTTAAAAAAGCTGCACTAGAGGGGGCTGAGGGGGTAAAAGCCAACGTTGAAGAAAGTACTGATCCTGAGAAGAGTAGTTCAATAGGCAAAGAAGCAGCTGGAAAAAATTTGGAACCGAACACTCCTCGAGAAAGGGAATTTGATTGTGATAGTGATAATCTAAACAATCCCAAAATCGAGCCAACGAAGGCGGAGGCAGAAGCTATTGCCAAGGGATTGCAGACAATAAGGAACGATGATCTAGAGGAGATCCGAGAATTAGGTTCTGGGACTTATGGGGCTGTGTATCATGGGAAGTGGAAGGGGTCTGACATAGCGATAAAGAGAATAAAATCCAGTTGTTTTGCTGGGAAACCATCAGAAAGAGAACGTTTGATTGCAGATTTCTGGAAGGAGGCTTTGATACTGAGTTCATTGCATCACCCAAATGTTGTTTCCATGTATGGTATTGTGCGCAATGGCCCTGATGGATCTTTAGCAACTGTGACTGAATTCATGGTTAATGGTTCTTTGAAACAGTTTTTGCAGAAAAAAGATCGAACAATCGATCGTCGTAAAAGATTTATCATAGCTATGGATGCTGCATTTGGGATGGAGTACCTGCATGGAAAAAATATAGTACATTTTGATTTAAAGTGTGAAAATCTGTTAGTGAATATGAGAGATCCACAGCGGCCTGTATGCAAGATTGGTGATTTGGGATTATCCAAAGTAAAACAACAGACTTTAGTGTCTGGAGGTGTTCGTGGAACTTTACCCTGGATGGCACCTGAGCTTCTTAGCGGGAAAAGTAACATGGTAACAGAGAAGATTGATGTTTACTCATTTGGGATTGTTATGTGGGAACTGCTTACGGGAGAAGAGCCATATACAGATATGCATTGTGCGTCTATAATTGGAGGTATTGTGAACAACACGTTGCGCCCTCAAATTCCCACGTGGTGCGACCCAGAATGGAAGTCTTTGATGGAAAGTTGTTGGGCCTCTGATCCTTCCCACAGGCCATCGTTTTCTGAAATTTCTCAGAAGCTAAGAAATATGGCTGCTGCAATGAATGTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1107

Amino Acids

122.83

Weight (kDa)

5.54

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 67 - 151 2.3e-18 PB1 domain
Pkinase PF00069 834 - 1094 1.4e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 835 - 1096 1.5e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2069
AasI GACNNNNNNGTC 1 cut(s) 2986
Acc16I TGCGCA 1 cut(s) 2711
Acc36I ACCTGC 2 cut(s) 1144, 2865
Acc65I GGTACC 1 cut(s) 870
AccB1I GGYRCC 2 cut(s) 870, 3022
AccB7I CCANNNNNTGG 1 cut(s) 203
AccI GTMKAC 2 cut(s) 522, 2192
AccII CGCG 2 cut(s) 151, 235
AciI CCGC 6 cut(s) 84, 149, 193, 2447, 2929, 3039
AclI AACGTT 3 cut(s) 1038, 2301, 2626
AclWI GGATC 9 cut(s) 421, 733, 1370, 1654, 2312, 2500, 2734, 2915, 3239
AcoI YGGCCR 1 cut(s) 1435
AcsI RAATTY 9 cut(s) 554, 1405, 2125, 2131, 2362, 2393, 2747, 3186, 3273
AcuI CTGAAG 1 cut(s) 942
AcvI CACGTG 1 cut(s) 3195
AdeI CACNNNGTG 1 cut(s) 3198
AfaI GTAC 8 cut(s) 65, 323, 872, 1180, 1533, 2314, 2855, 2877
AfiI CCNNNNNNNGG 8 cut(s) 98, 203, 220, 558, 722, 867, 1484, 1979
AflII CTTAAG 1 cut(s) 25
AflIII ACRYGT 1 cut(s) 3171
AjiI CACGTC 1 cut(s) 1745
AjnI CCWGG 5 cut(s) 465, 802, 1630, 2011, 3014
AjuI GAANNNNNNNTTGG 2 cut(s) 2674, 2706
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 5 cut(s) 224, 361, 1187, 1271, 1620
AlwI GGATC 9 cut(s) 421, 733, 1370, 1654, 2312, 2500, 2734, 2915, 3239
Ama87I CYCGRG 1 cut(s) 2382
AoxI GGCC 5 cut(s) 1435, 2716, 2930, 3237, 3257
ApoI RAATTY 9 cut(s) 554, 1405, 2125, 2131, 2362, 2393, 2747, 3186, 3273
AseI ATTAAT 2 cut(s) 48, 588
Asp700I GAANNNNTTC 2 cut(s) 1497, 1926
Asp718I GGTACC 1 cut(s) 870
AspLEI GCGC 3 cut(s) 1678, 2712, 3180
AspS9I GGNCC 8 cut(s) 340, 1259, 1329, 1394, 1418, 1475, 2717, 3237
AsuC2I CCSGG 1 cut(s) 1032
AsuHPI GGTGA 7 cut(s) 503, 1025, 1457, 2001, 2038, 2669, 2960
AvaI CYCGRG 1 cut(s) 2382
AvaII GGWCC 6 cut(s) 340, 1259, 1329, 1394, 1418, 1475
BaeGI GKGCMC 2 cut(s) 819, 1863
BalI TGGCCA 1 cut(s) 1437
BanI GGYRCC 2 cut(s) 870, 3022
BanII GRGCYC 1 cut(s) 1306
BauI CACGAG 1 cut(s) 2196
BbrPI CACGTG 1 cut(s) 3195
Bbv12I GWGCWC 1 cut(s) 318
BbvCI CCTCAGC 1 cut(s) 2283
BceAI ACGGC 1 cut(s) 865
BciT130I CCWGG 5 cut(s) 467, 804, 1632, 2013, 3016
BciVI GTATCC 1 cut(s) 579
BcnI CCSGG 1 cut(s) 1032
BcoDI GTCTC 5 cut(s) 224, 361, 1187, 1271, 1620
BfaI CTAG 4 cut(s) 432, 1919, 2273, 2498
BfmI CTRYAG 2 cut(s) 1005, 1545
BfrI CTTAAG 1 cut(s) 25
BfuAI ACCTGC 2 cut(s) 1144, 2865
BfuI GTATCC 1 cut(s) 579
BmcAI AGTACT 1 cut(s) 2314
Bme1390I CCNGG 6 cut(s) 467, 804, 1032, 1632, 2013, 3016
Bme18I GGWCC 6 cut(s) 340, 1259, 1329, 1394, 1418, 1475
BmeT110I CYCGRG 1 cut(s) 2382
BmgBI CACGTC 1 cut(s) 1745
BmgT120I GGNCC 8 cut(s) 340, 1259, 1329, 1394, 1418, 1475, 2717, 3237
BmiI GGNNCC 6 cut(s) 550, 872, 1158, 1396, 2370, 3024
BmrFI CCNGG 6 cut(s) 467, 804, 1032, 1632, 2013, 3016
BmsI GCATC 8 cut(s) 337, 466, 709, 817, 1204, 1700, 2683, 2824
BpmI CTGGAG 4 cut(s) 1377, 1827, 2034, 3012
Bpu10I CCTNAGC 3 cut(s) 437, 2283, 3027
BpuEI CTTGAG 4 cut(s) 302, 662, 692, 2135
BpuMI CCSGG 1 cut(s) 1032
Bsa29I ATCGAT 1 cut(s) 2802
BsaAI YACGTR 1 cut(s) 3195
BsaI GGTCTC 1 cut(s) 1620
BsaJI CCNNGG 7 cut(s) 681, 779, 803, 1332, 1757, 2466, 3014
BsaWI WCCGGW 2 cut(s) 213, 2239
BsaXI ACNNNNNCTCC 2 cut(s) 3147, 3177
Bsc4I CCNNNNNNNGG 8 cut(s) 98, 203, 220, 558, 722, 867, 1484, 1979
Bse1I ACTGG 1 cut(s) 2592
Bse3DI GCAATG 6 cut(s) 986, 1536, 1767, 2669, 2719, 3312
BseBI CCWGG 5 cut(s) 467, 804, 1632, 2013, 3016
BseCI ATCGAT 1 cut(s) 2802
BseDI CCNNGG 7 cut(s) 681, 779, 803, 1332, 1757, 2466, 3014
BseGI GGATG 7 cut(s) 457, 1098, 1438, 1691, 2839, 2853, 3024
BseLI CCNNNNNNNGG 8 cut(s) 98, 203, 220, 558, 722, 867, 1484, 1979
BseMI GCAATG 6 cut(s) 986, 1536, 1767, 2669, 2719, 3312
BseMII CTCAG 8 cut(s) 451, 564, 966, 2274, 2313, 2654, 3018, 3293
BseNI ACTGG 1 cut(s) 2592
BseRI GAGGAG 8 cut(s) 155, 284, 526, 1254, 1671, 1792, 2370, 2516
BseSI GKGCMC 2 cut(s) 819, 1863
BseYI CCCAGC 2 cut(s) 1361, 2603
BsgI GTGCAG 1 cut(s) 2253
Bsh1236I CGCG 2 cut(s) 151, 235
Bsh1285I CGRYCG 1 cut(s) 2806
BshFI GGCC 5 cut(s) 1437, 2718, 2932, 3239, 3259
BshNI GGYRCC 2 cut(s) 870, 3022
BshVI ATCGAT 1 cut(s) 2802
BsiEI CGRYCG 1 cut(s) 2806
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 2382
BsiSI CCGG 5 cut(s) 214, 1031, 1090, 1980, 2240
BslFI GGGAC 4 cut(s) 109, 562, 1380, 2539
BslI CCNNNNNNNGG 8 cut(s) 98, 203, 220, 558, 722, 867, 1484, 1979
BsmAI GTCTC 5 cut(s) 224, 361, 1187, 1271, 1620
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 4 cut(s) 109, 562, 1380, 2539
BsnI GGCC 5 cut(s) 1437, 2718, 2932, 3239, 3259
Bso31I GGTCTC 1 cut(s) 1620
BsoBI CYCGRG 1 cut(s) 2382
Bsp1286I GDGCHC 4 cut(s) 318, 819, 1306, 1863
Bsp1407I TGTACA 1 cut(s) 1531
Bsp19I CCATGG 1 cut(s) 681
BspACI CCGC 6 cut(s) 84, 149, 193, 2447, 2929, 3039
BspANI GGCC 5 cut(s) 1437, 2718, 2932, 3239, 3259
BspCNI CTCAG 8 cut(s) 450, 565, 967, 2275, 2314, 2655, 3019, 3292
BspDI ATCGAT 1 cut(s) 2802
BspFNI CGCG 2 cut(s) 151, 235
BspHI TCATGA 3 cut(s) 1200, 1288, 1684
BspLI GGNNCC 6 cut(s) 550, 872, 1158, 1396, 2370, 3024
BspMI ACCTGC 2 cut(s) 1144, 2865
BspPI GGATC 9 cut(s) 421, 733, 1370, 1654, 2312, 2500, 2734, 2915, 3239
BspQI GCTCTTC 2 cut(s) 1545, 3111
BspT107I GGYRCC 2 cut(s) 870, 3022
BspTI CTTAAG 1 cut(s) 25
BspTNI GGTCTC 1 cut(s) 1620
BsrDI GCAATG 6 cut(s) 986, 1536, 1767, 2669, 2719, 3312
BsrGI TGTACA 1 cut(s) 1531
BsrI ACTGG 1 cut(s) 2592
BssECI CCNNGG 7 cut(s) 681, 779, 803, 1332, 1757, 2466, 3014
BssSI CACGAG 1 cut(s) 2196
BssT1I CCWWGG 4 cut(s) 681, 779, 1757, 2466
Bst2BI CACGAG 1 cut(s) 2196
Bst2UI CCWGG 5 cut(s) 467, 804, 1632, 2013, 3016
Bst4CI ACNGT 4 cut(s) 875, 1073, 2740, 2775
Bst6I CTCTTC 5 cut(s) 390, 1453, 1545, 2321, 3111
BstAFI CTTAAG 1 cut(s) 25
BstAUI TGTACA 1 cut(s) 1531
BstBAI YACGTR 1 cut(s) 3195
BstC8I GCNNGC 4 cut(s) 1671, 1863, 2145, 2167
BstDSI CCRYGG 1 cut(s) 681
BstENI CCTNNNNNAGG 1 cut(s) 720
BstF5I GGATG 7 cut(s) 457, 1098, 1438, 1691, 2839, 2853, 3024
BstFNI CGCG 2 cut(s) 151, 235
BstHHI GCGC 3 cut(s) 1678, 2712, 3180
BstMAI GTCTC 5 cut(s) 224, 361, 1187, 1271, 1620
BstMCI CGRYCG 1 cut(s) 2806
BstMWI GCNNNNNNNGC 5 cut(s) 657, 814, 1358, 1675, 2834
BstNI CCWGG 5 cut(s) 467, 804, 1632, 2013, 3016
BstNSI RCATGY 4 cut(s) 1352, 1628, 1640, 1673
BstSCI CCNGG 6 cut(s) 465, 802, 1030, 1630, 2011, 3014
BstSFI CTRYAG 2 cut(s) 1005, 1545
BstSLI GKGCMC 2 cut(s) 819, 1863
BstUI CGCG 2 cut(s) 151, 235
BstX2I RGATCY 5 cut(s) 413, 1659, 2505, 2726, 2920
BstYI RGATCY 5 cut(s) 413, 1659, 2505, 2726, 2920
Bsu15I ATCGAT 1 cut(s) 2802
BsuI GTATCC 1 cut(s) 579
BsuRI GGCC 5 cut(s) 1437, 2718, 2932, 3239, 3259
BsuTUI ATCGAT 1 cut(s) 2802
BtgI CCRYGG 1 cut(s) 681
BtgZI GCGATG 2 cut(s) 435, 462
BtrI CACGTC 1 cut(s) 1745
BtsCI GGATG 7 cut(s) 457, 1098, 1438, 1691, 2839, 2853, 3024
BtsI GCAGTG 3 cut(s) 111, 318, 1084
BtsIMutI CAGTG 3 cut(s) 111, 318, 1084
BveI ACCTGC 2 cut(s) 1144, 2865
Cac8I GCNNGC 4 cut(s) 1671, 1863, 2145, 2167
CciI TCATGA 3 cut(s) 1200, 1288, 1684
CfoI GCGC 3 cut(s) 1678, 2712, 3180
Cfr13I GGNCC 8 cut(s) 340, 1259, 1329, 1394, 1418, 1475, 2717, 3237
ClaI ATCGAT 1 cut(s) 2802
CpoI CGGWCCG 1 cut(s) 1329
CseI GACGC 3 cut(s) 241, 533, 3132
Csp6I GTAC 8 cut(s) 64, 322, 871, 1179, 1532, 2313, 2854, 2876
CspI CGGWCCG 1 cut(s) 1329
CviQI GTAC 8 cut(s) 64, 322, 871, 1179, 1532, 2313, 2854, 2876
DraI TTTAAA 3 cut(s) 540, 2208, 2889
DraIII CACNNNGTG 1 cut(s) 3198
DrdI GACNNNNNNGTC 1 cut(s) 2986
DseDI GACNNNNNNGTC 1 cut(s) 2986
EaeI YGGCCR 1 cut(s) 1435
Eam1104I CTCTTC 5 cut(s) 390, 1453, 1545, 2321, 3111
EarI CTCTTC 5 cut(s) 390, 1453, 1545, 2321, 3111
EciI GGCGGA 1 cut(s) 2462
Eco130I CCWWGG 4 cut(s) 681, 779, 1757, 2466
Eco24I GRGCYC 1 cut(s) 1306
Eco31I GGTCTC 1 cut(s) 1620
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 6 cut(s) 340, 1259, 1329, 1394, 1418, 1475
Eco57I CTGAAG 1 cut(s) 942
Eco72I CACGTG 1 cut(s) 3195
Eco88I CYCGRG 1 cut(s) 2382
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 2747
EcoRII CCWGG 5 cut(s) 465, 802, 1630, 2011, 3014
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 4 cut(s) 681, 779, 1757, 2466
EcoT22I ATGCAT 2 cut(s) 1671, 3138
EcoT38I GRGCYC 1 cut(s) 1306
ErhI CCWWGG 4 cut(s) 681, 779, 1757, 2466
Esp3I CGTCTC 1 cut(s) 224
FaqI GGGAC 4 cut(s) 109, 562, 1380, 2539
FauI CCCGC 2 cut(s) 200, 3032
FauNDI CATATG 1 cut(s) 961
FblI GTMKAC 2 cut(s) 522, 2192
FokI GGATG 7 cut(s) 444, 1105, 1445, 1678, 2846, 2860, 3031
FriOI GRGCYC 1 cut(s) 1306
FspBI CTAG 4 cut(s) 432, 1919, 2273, 2498
FspI TGCGCA 1 cut(s) 2711
GlaI GCGC 3 cut(s) 1677, 2711, 3179
GsaI CCCAGC 2 cut(s) 1365, 2607
GsuI CTGGAG 4 cut(s) 1377, 1827, 2034, 3012
HaeIII GGCC 5 cut(s) 1437, 2718, 2932, 3239, 3259
HapII CCGG 5 cut(s) 214, 1031, 1090, 1980, 2240
HgaI GACGC 3 cut(s) 241, 533, 3132
HhaI GCGC 3 cut(s) 1678, 2712, 3180
Hin6I GCGC 3 cut(s) 1676, 2710, 3178
HinP1I GCGC 3 cut(s) 1676, 2710, 3178
HincII GTYRAC 2 cut(s) 523, 1798
HindII GTYRAC 2 cut(s) 523, 1798
HindIII AAGCTT 2 cut(s) 2141, 2210
HpaII CCGG 5 cut(s) 214, 1031, 1090, 1980, 2240
HphI GGTGA 7 cut(s) 503, 1025, 1457, 2001, 2038, 2669, 2960
Hpy99I CGWCG 3 cut(s) 527, 1979, 2811
HpyCH4III ACNGT 4 cut(s) 875, 1073, 2740, 2775
HpyCH4IV ACGT 6 cut(s) 1038, 1744, 2301, 2626, 3173, 3194
HpyF10VI GCNNNNNNNGC 5 cut(s) 657, 814, 1358, 1675, 2834
HpySE526I ACGT 6 cut(s) 1038, 1744, 2301, 2626, 3173, 3194
HspAI GCGC 3 cut(s) 1676, 2710, 3178
KpnI GGTACC 1 cut(s) 874
LguI GCTCTTC 2 cut(s) 1545, 3111
LmnI GCTCC 7 cut(s) 292, 554, 781, 1001, 1358, 1546, 1708
LweI GCATC 8 cut(s) 337, 466, 709, 817, 1204, 1700, 2683, 2824
MaeI CTAG 4 cut(s) 432, 1919, 2273, 2498
MaeII ACGT 6 cut(s) 1038, 1744, 2301, 2626, 3173, 3194
MaeIII GTNAC 8 cut(s) 5, 29, 379, 1034, 2177, 2740, 3047, 3055
MflI RGATCY 5 cut(s) 413, 1659, 2505, 2726, 2920
MhlI GDGCHC 4 cut(s) 318, 819, 1306, 1863
MlsI TGGCCA 1 cut(s) 1437
MluNI TGGCCA 1 cut(s) 1437
MlyI GAGTC 6 cut(s) 26, 584, 793, 1273, 1793, 2126
MmeI TCCRAC 2 cut(s) 1307, 1974
Mox20I TGGCCA 1 cut(s) 1437
Mph1103I ATGCAT 2 cut(s) 1671, 3138
MroXI GAANNNNTTC 2 cut(s) 1497, 1926
MscI TGGCCA 1 cut(s) 1437
MslI CAYNNNNRTG 4 cut(s) 1272, 1623, 2682, 2828
Msp20I TGGCCA 1 cut(s) 1437
MspA1I CMGCKG 2 cut(s) 2354, 2929
MspCI CTTAAG 1 cut(s) 25
MspI CCGG 5 cut(s) 214, 1031, 1090, 1980, 2240
MspR9I CCNGG 6 cut(s) 467, 804, 1032, 1632, 2013, 3016
MvaI CCWGG 5 cut(s) 467, 804, 1632, 2013, 3016
MvnI CGCG 2 cut(s) 151, 235
MwoI GCNNNNNNNGC 5 cut(s) 657, 814, 1358, 1675, 2834
NciI CCSGG 1 cut(s) 1032
NcoI CCATGG 1 cut(s) 681
NdeI CATATG 1 cut(s) 961
NlaIV GGNNCC 6 cut(s) 550, 872, 1158, 1396, 2370, 3024
NmuCI GTSAC 4 cut(s) 29, 379, 2177, 2740
NsbI TGCGCA 1 cut(s) 2711
NsiI ATGCAT 2 cut(s) 1671, 3138
NspI RCATGY 4 cut(s) 1352, 1628, 1640, 1673
PaeI GCATGC 1 cut(s) 1673
PaeR7I CTCGAG 1 cut(s) 2382
PagI TCATGA 3 cut(s) 1200, 1288, 1684
PciSI GCTCTTC 2 cut(s) 1545, 3111
PdmI GAANNNNTTC 2 cut(s) 1497, 1926
PfeI GAWTC 4 cut(s) 145, 824, 946, 1207
PflMI CCANNNNNTGG 1 cut(s) 203
Ple19I CGATCG 1 cut(s) 2806
PleI GAGTC 6 cut(s) 26, 583, 792, 1273, 1793, 2125
PmaCI CACGTG 1 cut(s) 3195
PmlI CACGTG 1 cut(s) 3195
PpsI GAGTC 6 cut(s) 26, 583, 792, 1273, 1793, 2125
Ppu21I YACGTR 1 cut(s) 3195
PpuMI RGGWCCY 1 cut(s) 340
PshBI ATTAAT 2 cut(s) 48, 588
PsiI TTATAA 1 cut(s) 2069
Psp1406I AACGTT 3 cut(s) 1038, 2301, 2626
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 5 cut(s) 465, 802, 1630, 2011, 3014
PspCI CACGTG 1 cut(s) 3195
PspFI CCCAGC 2 cut(s) 1361, 2603
PspGI CCWGG 5 cut(s) 465, 802, 1630, 2011, 3014
PspN4I GGNNCC 6 cut(s) 550, 872, 1158, 1396, 2370, 3024
PspPI GGNCC 8 cut(s) 340, 1259, 1329, 1394, 1418, 1475, 2717, 3237
PspPPI RGGWCCY 1 cut(s) 340
PsuI RGATCY 5 cut(s) 413, 1659, 2505, 2726, 2920
PvuI CGATCG 1 cut(s) 2806
PvuII CAGCTG 1 cut(s) 2354
RsaI GTAC 8 cut(s) 65, 323, 872, 1180, 1533, 2314, 2855, 2877
RsaNI GTAC 8 cut(s) 64, 322, 871, 1179, 1532, 2313, 2854, 2876
RseI CAYNNNNRTG 4 cut(s) 1272, 1623, 2682, 2828
Rsr2I CGGWCCG 1 cut(s) 1329
RsrII CGGWCCG 1 cut(s) 1329
SalI GTCGAC 1 cut(s) 521
SapI GCTCTTC 2 cut(s) 1545, 3111
Sau96I GGNCC 8 cut(s) 340, 1259, 1329, 1394, 1418, 1475, 2717, 3237
ScaI AGTACT 1 cut(s) 2314
SchI GAGTC 6 cut(s) 26, 584, 793, 1273, 1793, 2126
ScrFI CCNGG 6 cut(s) 467, 804, 1032, 1632, 2013, 3016
SduI GDGCHC 4 cut(s) 318, 819, 1306, 1863
SfaNI GCATC 8 cut(s) 337, 466, 709, 817, 1204, 1700, 2683, 2824
SfcI CTRYAG 2 cut(s) 1005, 1545
Sfr274I CTCGAG 1 cut(s) 2382
SinI GGWCC 6 cut(s) 340, 1259, 1329, 1394, 1418, 1475
SlaI CTCGAG 1 cut(s) 2382
SmiMI CAYNNNNRTG 4 cut(s) 1272, 1623, 2682, 2828
SmlI CTYRAG 6 cut(s) 25, 317, 641, 707, 2114, 2382
SmoI CTYRAG 6 cut(s) 25, 317, 641, 707, 2114, 2382
SphI GCATGC 1 cut(s) 1673
SsiI CCGC 6 cut(s) 84, 149, 193, 2447, 2929, 3039
SspI AATATT 3 cut(s) 1603, 1789, 2032
SspMI CTAG 4 cut(s) 432, 1919, 2273, 2498
StyD4I CCNGG 6 cut(s) 465, 802, 1030, 1630, 2011, 3014
StyI CCWWGG 4 cut(s) 681, 779, 1757, 2466
TaaI ACNGT 4 cut(s) 875, 1073, 2740, 2775
TaiI ACGT 6 cut(s) 1041, 1747, 2304, 2629, 3176, 3197
TaqI TCGA 7 cut(s) 267, 522, 2120, 2383, 2433, 2794, 2802
TaqII GACCGA 1 cut(s) 1346
TatI WGTACW 4 cut(s) 63, 1531, 2312, 2875
TauI GCSGC 1 cut(s) 2932
TfiI GAWTC 4 cut(s) 145, 824, 946, 1207
TscAI CASTG 3 cut(s) 118, 318, 1091
TseFI GTSAC 4 cut(s) 29, 379, 2177, 2740
Tsp45I GTSAC 4 cut(s) 29, 379, 2177, 2740
TspGWI ACGGA 2 cut(s) 1684, 2075
TspRI CASTG 3 cut(s) 118, 318, 1091
Van91I CCANNNNNTGG 1 cut(s) 203
Vha464I CTTAAG 1 cut(s) 25
VpaK11BI GGWCC 6 cut(s) 340, 1259, 1329, 1394, 1418, 1475
VspI ATTAAT 2 cut(s) 48, 588
XagI CCTNNNNNAGG 1 cut(s) 720
XapI RAATTY 9 cut(s) 554, 1405, 2125, 2131, 2362, 2393, 2747, 3186, 3273
XbaI TCTAGA 2 cut(s) 1918, 2497
XceI RCATGY 4 cut(s) 1352, 1628, 1640, 1673
XhoI CTCGAG 1 cut(s) 2382
XmiI GTMKAC 2 cut(s) 522, 2192
XmnI GAANNNNTTC 2 cut(s) 1497, 1926
XspI CTAG 4 cut(s) 432, 1919, 2273, 2498
ZrmI AGTACT 1 cut(s) 2314
Zsp2I ATGCAT 2 cut(s) 1671, 3138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.