RchiOBHm_Chr4g0403261

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005070
Physical Location & Seq
Forward (+)
22351721 .. 22353477
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37502

Sequence Viewer

Length: 771 bp
ATGAGCAAGATGAGGGAGCTTTATGAAGGGGCGGCGGTGCTTAAGTACCAGCAACCGGATGAGGACCTTGACGCCCTTGTCTCGGTTGTGAATGATGATGATGTGACCAACATGATGGAAGAGTATGATAAGTTGGGGTCGGGTGATGGTTTCACCAGGCTCAGGATTTTTTTGTTTTCGTATCCCGACCAAGATGGTGGTTCCTCACACTATGACGGGGATGAAAGGGATCATGAGAGGAGGTATGTTGATGCACTCAATAACTTAAATGATGGTTCCGAATTTAGGAAGCAGCATTTAGAGTCCCCTGTCATCACTTCAGTTGATGATCTCCATGGAGTTGAACAGTTCTTTAGTCCAATGAGTCTCGAGGGTGGCCTCCATAGCCAGAGAAACGAGATATCCATGTCTCATTATAACCTGCATCGCCTCAAAATACCTCATATAGGTTCAGGGCAACACCATCAGCCCATTAGCCAAAGGTATAACGACATGGAAGCTCCGTGGAGTCCTGCATACCATTCTCCTAGGCATCACGGGCACCTTGATCCAAGACCAATGGCAGAGTTTCCAATTTCTCCTACCTCTGCACGGTACCGTATGCCATTTGCAGATGTACCTGATAAATGTTCAGATAGAATGCCTGAAGAATATGCACGGCCGCCATTGAATCATCAGCCTGGATTTCATCAGTATTCTGATAATGCTGTATGGCTTCCATCTCACTTTGAAGAGAGGAATGCATATAATGCTTGTTTTGCTGTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

256

Amino Acids

29.44

Weight (kDa)

5.26

Isoelectric Point (pI)

57.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 2 - 59 1.2e-08 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 429
Acc65I GGTACC 1 cut(s) 594
AccB1I GGYRCC 2 cut(s) 540, 594
AciI CCGC 3 cut(s) 32, 35, 662
AclWI GGATC 2 cut(s) 237, 542
AcoI YGGCCR 1 cut(s) 659
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 2 cut(s) 303, 666
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 47, 596, 618
AfiI CCNNNNNNNGG 6 cut(s) 55, 82, 162, 285, 446, 591
AflII CTTAAG 1 cut(s) 41
AgsI TTSAA 3 cut(s) 344, 670, 731
AjnI CCWGG 2 cut(s) 155, 679
AluBI AGCT 2 cut(s) 19, 500
AluI AGCT 2 cut(s) 19, 500
Alw26I GTCTC 3 cut(s) 85, 371, 414
AlwI GGATC 2 cut(s) 237, 542
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 2 cut(s) 376, 659
ApeKI GCWGC 1 cut(s) 292
ApoI RAATTY 1 cut(s) 281
Asp718I GGTACC 1 cut(s) 594
AspA2I CCTAGG 1 cut(s) 527
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 2 cut(s) 145, 155
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 64
AvrII CCTAGG 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 543
BanI GGYRCC 2 cut(s) 540, 594
BbvI GCAGC 1 cut(s) 304
BccI CCATC 6 cut(s) 109, 140, 188, 266, 471, 727
BceAI ACGGC 1 cut(s) 674
BciT130I CCWGG 2 cut(s) 157, 681
BciVI GTATCC 1 cut(s) 192
BcoDI GTCTC 3 cut(s) 85, 371, 414
BfaI CTAG 1 cut(s) 528
BfrI CTTAAG 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 429
BfuI GTATCC 1 cut(s) 192
BisI GCNGC 3 cut(s) 33, 293, 662
BlnI CCTAGG 1 cut(s) 527
BlsI GCNGC 3 cut(s) 34, 294, 663
Bme1390I CCNGG 2 cut(s) 157, 681
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 4 cut(s) 202, 277, 542, 596
BmrFI CCNGG 2 cut(s) 157, 681
BmsI GCATC 3 cut(s) 241, 433, 541
Bpu10I CCTNAGC 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 334, 503, 527
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 6 cut(s) 55, 82, 162, 285, 446, 591
BseBI CCWGG 2 cut(s) 157, 681
BseDI CCNNGG 3 cut(s) 334, 503, 527
BseGI GGATG 2 cut(s) 64, 226
BseLI CCNNNNNNNGG 6 cut(s) 55, 82, 162, 285, 446, 591
BseMII CTCAG 1 cut(s) 175
BseRI GAGGAG 1 cut(s) 253
BseSI GKGCMC 1 cut(s) 543
BseX3I CGGCCG 1 cut(s) 659
BseXI GCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 573
Bsh1285I CGRYCG 1 cut(s) 662
BshFI GGCC 2 cut(s) 378, 661
BshNI GGYRCC 2 cut(s) 540, 594
BsiEI CGRYCG 1 cut(s) 662
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 1 cut(s) 56
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 6 cut(s) 55, 82, 162, 285, 446, 591
BsmAI GTCTC 3 cut(s) 85, 371, 414
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 2 cut(s) 645, 745
BsnI GGCC 2 cut(s) 378, 661
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 3 cut(s) 229, 328, 547
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 3 cut(s) 32, 35, 662
BspANI GGCC 2 cut(s) 378, 661
BspCNI CTCAG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 4 cut(s) 202, 277, 542, 596
BspMI ACCTGC 1 cut(s) 429
BspPI GGATC 2 cut(s) 237, 542
BspT107I GGYRCC 2 cut(s) 540, 594
BspTI CTTAAG 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 334, 503, 527
BssMI GATC 3 cut(s) 229, 328, 547
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 334, 527
Bst2UI CCWGG 2 cut(s) 157, 681
Bst4CI ACNGT 3 cut(s) 348, 594, 599
Bst6I CTCTTC 2 cut(s) 114, 726
BstACI GRCGYC 1 cut(s) 72
BstAFI CTTAAG 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 749
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 2 cut(s) 334, 503
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 2 cut(s) 64, 226
BstKTI GATC 3 cut(s) 232, 331, 550
BstMAI GTCTC 3 cut(s) 85, 371, 414
BstMBI GATC 3 cut(s) 229, 328, 547
BstMCI CGRYCG 1 cut(s) 662
BstMWI GCNNNNNNNGC 4 cut(s) 384, 538, 749, 758
BstNI CCWGG 2 cut(s) 157, 681
BstSCI CCNGG 2 cut(s) 155, 679
BstSLI GKGCMC 1 cut(s) 543
BstV1I GCAGC 1 cut(s) 304
BstXI CCANNNNNNTGG 2 cut(s) 115, 197
BstZI CGGCCG 1 cut(s) 659
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 2 cut(s) 378, 661
BtgI CCRYGG 2 cut(s) 334, 503
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 2 cut(s) 64, 226
BveI ACCTGC 1 cut(s) 429
CciI TCATGA 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 64
CseI GACGC 1 cut(s) 80
Csp6I GTAC 3 cut(s) 46, 595, 617
CviAII CATG 5 cut(s) 112, 233, 335, 406, 493
CviQI GTAC 3 cut(s) 46, 595, 617
DdeI CTNAG 1 cut(s) 161
DpnI GATC 3 cut(s) 231, 330, 549
DpnII GATC 3 cut(s) 229, 328, 547
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 659
EagI CGGCCG 1 cut(s) 659
Eam1104I CTCTTC 2 cut(s) 114, 726
EarI CTCTTC 2 cut(s) 114, 726
EclXI CGGCCG 1 cut(s) 659
Eco130I CCWWGG 2 cut(s) 334, 527
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 64
Eco52I CGGCCG 1 cut(s) 659
Eco57I CTGAAG 2 cut(s) 303, 666
Eco88I CYCGRG 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 155, 679
EcoRV GATATC 1 cut(s) 402
EcoT14I CCWWGG 2 cut(s) 334, 527
EcoT22I ATGCAT 1 cut(s) 745
ErhI CCWWGG 2 cut(s) 334, 527
FaeI CATG 5 cut(s) 115, 236, 338, 409, 496
FaqI GGGAC 1 cut(s) 289
FatI CATG 5 cut(s) 111, 232, 334, 405, 492
Fnu4HI GCNGC 3 cut(s) 33, 293, 662
FokI GGATG 2 cut(s) 71, 233
Fsp4HI GCNGC 3 cut(s) 33, 293, 662
FspBI CTAG 1 cut(s) 528
GluI GCNGC 3 cut(s) 33, 293, 662
HaeIII GGCC 2 cut(s) 378, 661
HapII CCGG 1 cut(s) 56
HgaI GACGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 5 cut(s) 115, 236, 338, 409, 496
HinfI GANTC 4 cut(s) 302, 364, 508, 670
HpaII CCGG 1 cut(s) 56
HphI GGTGA 2 cut(s) 145, 155
Hpy188I TCNGA 3 cut(s) 280, 634, 700
Hpy188III TCNNGA 4 cut(s) 163, 185, 233, 368
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 3 cut(s) 348, 594, 599
HpyCH4V TGCA 7 cut(s) 254, 424, 515, 590, 611, 656, 743
HpyF10VI GCNNNNNNNGC 4 cut(s) 384, 538, 749, 758
HpyF3I CTNAG 1 cut(s) 161
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 5 cut(s) 115, 236, 338, 409, 496
KpnI GGTACC 1 cut(s) 598
Kzo9I GATC 3 cut(s) 229, 328, 547
LmnI GCTCC 2 cut(s) 16, 505
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 3 cut(s) 241, 433, 541
MaeI CTAG 1 cut(s) 528
MaeIII GTNAC 1 cut(s) 103
MalI GATC 3 cut(s) 231, 330, 549
MboI GATC 3 cut(s) 229, 328, 547
MboII GAAGA 3 cut(s) 131, 659, 743
MhlI GDGCHC 1 cut(s) 543
MluCI AATT 2 cut(s) 281, 573
MlyI GAGTC 3 cut(s) 311, 373, 517
Mph1103I ATGCAT 1 cut(s) 745
MseI TTAA 2 cut(s) 42, 266
MspCI CTTAAG 1 cut(s) 41
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 2 cut(s) 157, 681
Mva1269I GAATGC 2 cut(s) 645, 745
MvaI CCWGG 2 cut(s) 157, 681
MwoI GCNNNNNNNGC 4 cut(s) 384, 538, 749, 758
NcoI CCATGG 1 cut(s) 334
NdeII GATC 3 cut(s) 229, 328, 547
NlaIII CATG 5 cut(s) 115, 236, 338, 409, 496
NlaIV GGNNCC 4 cut(s) 202, 277, 542, 596
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 745
PaeR7I CTCGAG 1 cut(s) 368
PagI TCATGA 1 cut(s) 232
PctI GAATGC 2 cut(s) 645, 745
PfeI GAWTC 1 cut(s) 670
PkrI GCNGC 3 cut(s) 34, 294, 663
PleI GAGTC 3 cut(s) 310, 372, 516
PpsI GAGTC 3 cut(s) 310, 372, 516
PpuMI RGGWCCY 1 cut(s) 64
PsiI TTATAA 1 cut(s) 417
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 155, 679
PspGI CCWGG 2 cut(s) 155, 679
PspN4I GGNNCC 4 cut(s) 202, 277, 542, 596
PspPI GGNCC 1 cut(s) 64
PspPPI RGGWCCY 1 cut(s) 64
RsaI GTAC 3 cut(s) 47, 596, 618
RsaNI GTAC 3 cut(s) 46, 595, 617
SaqAI TTAA 2 cut(s) 42, 266
SatI GCNGC 3 cut(s) 33, 293, 662
Sau3AI GATC 3 cut(s) 229, 328, 547
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 3 cut(s) 311, 373, 517
ScrFI CCNGG 2 cut(s) 157, 681
SduI GDGCHC 1 cut(s) 543
SfaNI GCATC 3 cut(s) 241, 433, 541
Sfr274I CTCGAG 1 cut(s) 368
SinI GGWCC 1 cut(s) 64
SlaI CTCGAG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 41, 368
SmoI CTYRAG 2 cut(s) 41, 368
Sse9I AATT 2 cut(s) 281, 573
SsiI CCGC 3 cut(s) 32, 35, 662
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 2 cut(s) 155, 679
StyI CCWWGG 2 cut(s) 334, 527
TaaI ACNGT 3 cut(s) 348, 594, 599
TaqI TCGA 1 cut(s) 369
TasI AATT 2 cut(s) 281, 573
TauI GCSGC 2 cut(s) 35, 664
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 2 cut(s) 42, 266
Tru9I TTAA 2 cut(s) 42, 266
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 39, 237, 677
TspGWI ACGGA 1 cut(s) 492
Vha464I CTTAAG 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 368
XmaJI CCTAGG 1 cut(s) 527
XspI CTAG 1 cut(s) 528
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.