MD13G1266700.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005070
Physical Location & Seq
Reverse (-)
34704682 .. 34711335
6654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1266700.v1.1.491

Sequence Viewer

Length: 3339 bp
ATGTGTAACAAAGGAATTGCCTGCTTGAGCGACTCCGACTCCGACTCCGAAAACCCAATTGATCATCAGGCTGTGTACTTGATGGATAGCCCTTCGGCTACCCCGCACTTTGGTTCTAATTCCACCGCTGCTTCGTCCCCCAATTCGAATGAGGAAAACCCGCGTGTTAAGTTTCTGTGTAGTTTTTCGGGGAGTATATTGCCCCGCCCACAAGATGGGAAGCTCCGTTATGTGGGAGGAGAGACGCGAATTGTGAGTGTTCCACGAGATATCAAGTTTGATGAGTTGATTAACAAGATGAGGGAGCTTTATGAAGGTGCAGCAGTGCTCAAGTATCAGCAACCCGATGAGGACCTTGATGCTCTTGTCTCAGTTGTGAACAATGATGATGTGACTAACATGATGGAAGAGTATGATAAGTTGGGATCTGGCGATGGTTTCACTAGGCTCAGGATTTTTTTGTTTTCGCATCCCGACCAAGACAGCTCATCGCACTATGAGGGTGATGAAAGGGATAATGAGAGGAGGTATGTCGATGCTCTCAATAATTTAAATGATAGCTCCGAATTTAGGAAGCAATATCCCGAGTCCCCTCTTACTAATCCGGTTGATGACCTCCATATAGCAGAACAATTTTTTAGTCCAATGAGTCTTGAGGGTGGCCTTCACAGTCAGAGAAATTGTGATATATCCATGGCTCAATACAACTTGCATCAGCTCAAGATACCTCATGCAGGATCGGGGCAACATCATCAACCAATTAGTCAGAGGTATAATGAAATGGAAGCTCCTTGGAGTCCTGCATACTATTCTCCCAGGCACCATGCGCACCTTGATCCAAGACCAATGGCTGAGTTTCCTTCCTCTCCTTCCTCTGCACGGTACTGTATGCCATTTCCAGATGTACCCGATAAATGTTTAGATAGAATGCCTGAAGAATATGCACGACAGCCATTGAATCACCAGCCTCCATATGAACACCAACCACAGTATTCTGAGAATGTTGTATGGCTTCCAAGTGGAGCAATAGGTGGTGAAAGGTCAGGATTTCCAGGTAACATTTTTCATGGAAACAATGCCGTCGAAGGAAACAGTATTTGTGAACACTGCCAGATGACTTTCCAGAGAAACCAACCACATTTTGAGCAACCAATCGTGGCAAATGGATTTCAGCATGTTGCTAATCCAAGCGGCAAGTGTACGCCAAATAGAGAGACTTTCGTGATGAATCCAGATGCAAAGTTGCACCATGAAATATACGTAAGTGAACAGAATACTGGTCCTCATCATTTTAGTGAGACTCCAAATCATGAAAGAGGCTGGACTCCACATCTTCATTTGAATTGCCGGACTGAGGAAGCACGACCACATGCTTCTGGAGCTGGGAAATTGAATGATCCTTACATTGTAGATGGTCACATAAATTTGCCTCTTGGTCCTAATACTGTGGATGACCATCACGTCACTTCCAATTATGTTCATCACCCAGCTGGACCTGAAATGGGAAATGAAGTGTTTCATGAACGATCTGTGGCTGCTCCACCCCATGTACACATTGCTCCTGTAGAAGAGTATGGTGTTCGTTATGGAAATTTTCCTTATCCCTATGGAGGAGATAATATTTATCGGGGGTCTCATGGACATGCAACTGGACCGGCAGTATGGAGAAATGTTCAGAGCCCAATGCATGCTGCGCCCCCTTACGAAGCATCCATTTCTGCTCCACAAGTGAATGGTTCTGTTAATGTGGGATATCTCAGACGTGAAGATAGTCCAGTGTTTTCCATTGGATTAGACAATCAAAATATTTGGGTTGACACCTCGCAGGAAATGTTAGGTTTGGAAGGAAAAGCTGTTCCTCCGGACTGTTCTTATGGGCACGCTGTGAAGTTGAACCCAAACCCACTCTGTCAAGAAAATCATCAAGCATATCCTCCAGACCGCGTTCAACCTACACCTGATATGATAAATTGTGCTATTCCTTTGGACCCCAACTCCGTTGTAAGGTTTGAGGAAAAGAGTTCACCTGGAGTAAATGAGGTGAATCTTGTGGACAACGTGGAGAACCCTGAAACAGAGGTTATAAATCCAAACAATCATTGTGATAAAAATGGTGGTGTGGTATCTCTTGAGTCAATAAATTCGAATTTTGCTAAGCTTGCAGAAGAAAGTGGTAATGTTGGCAAAACAAGTGACGAGGATCAGTCTACTTGTGAACTTTCGAAGCTTTCTGTTAACAATTTGAGTTTCATACCGGAATTGATTGCTTCTGTTAAAAAAGCTGCTCTAGAGGGGGCTGAGGAGGTGAAAACCAATGCTGAAGAAAGTACCGACCCTGAGAAGAGTAGTTCAATAGACAAAGAAGCAGCTGCAAAAAATTTGGAACAACCGAACACTCCTGGAGACAGGGAATTGGATTCTGATAGTGATAATCTAAACAATTCCAAAATTGAGCCAACAAGGGCCGAGGCAGAAGCTATTGCCAAGGGATTGCAGACAATAAGGAATGATGATCTTGAGGAGATCCGAGAATTAGGTTCTGGGACTTACGGGGCTGTGTTTCATGGGAAGTGGAAGGGGTCTGATGTAGCAATAAAGAGAATAAAGTCCAGTTGTTTTGCTGGGAGACCATCAGAAAGAGAACGTTTGATTGCGGATTTCTGGAAGGAGGCTTTAATACTGAGTTCATTGCATCACCCAAATGTTGTTTCAATGTATGGTATTGTGCGAGATGGCCCTGATGGATCTTTAGCAACTGTGACCGAATTCATGGTTAATGGTTCTTTGAAACAGTTTTTGCAGAAAAAAGACCGAACAATTGATCGTCGTAAAAGATTGATCATAGCTATGGATGCTGCATTTGGGATGGAGTATCTGCATGGAAAAAATATTGTGCATTTTGATTTAAAGTGTGAAAATCTATTAGTAAATATGAGAGATCCACAACGGCCTGTATGCAAGATTGGTGATTTGGGATTATCCAAAGTAAAACAACAGACTTTAGTGTCTGGAGGTGTTCGTGGAACTTTGCCCTGGATGGCACCTGAGCTTCTTAGCGGGAAAAGTAACATGGTAACAGAGAAGATTGATGTTTACTCATTTGGAATTGTTATGTGGGAACTGCTTACGGGAGATGAGCCATATAGAGATATGCATTGTGCTTCTATAATTGGAGGTATAGTGAACAACACTCTGCGTCCTCAAATTCCCCCGTGGTGCGATCCAGAATGGAAGTCTTTGATGGAAAGTTGTTGGGCTCCTGAGCCTTCACAGAGGCCATCGTTTTCTGAAATCTCTCAGAAGCTAAGGAATATGGCTGCTGCAATGAATGTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1113

Amino Acids

123.64

Weight (kDa)

5.3

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 71 - 155 7.7e-17 PB1 domain
Pkinase PF00069 840 - 1100 5e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 841 - 1102 1.6e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2084
AasI GACNNNNNNGTC 2 cut(s) 1460, 3004
Acc16I TGCGCA 1 cut(s) 828
AccB1I GGYRCC 2 cut(s) 819, 3040
AccB7I CCANNNNNTGG 1 cut(s) 215
AccI GTMKAC 1 cut(s) 2207
AccII CGCG 3 cut(s) 163, 247, 1944
AccIII TCCGGA 1 cut(s) 1861
AciI CCGC 8 cut(s) 104, 126, 161, 205, 1191, 1942, 2654, 3057
AclI AACGTT 1 cut(s) 2644
AclWI GGATC 9 cut(s) 433, 745, 830, 1391, 2208, 2518, 2752, 2933, 3215
AcsI RAATTY 8 cut(s) 566, 1423, 1591, 2140, 2146, 2377, 2765, 3204
AcuI CTGAAG 2 cut(s) 954, 2340
AdeI CACNNNGTG 1 cut(s) 1885
AfaI GTAC 6 cut(s) 77, 884, 906, 1201, 1551, 2329
AgsI TTSAA 8 cut(s) 958, 1342, 1393, 1894, 1949, 2352, 2712, 2788
AjiI CACGTC 2 cut(s) 1462, 1763
AjnI CCWGG 5 cut(s) 815, 1051, 2026, 2398, 3032
AjuI GAANNNNNNNTTGG 2 cut(s) 2692, 2724
AloI GAACNNNNNNTCC 2 cut(s) 1839, 1871
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 7 cut(s) 236, 373, 1208, 1292, 1638, 2397, 2620
AlwI GGATC 9 cut(s) 433, 745, 830, 1391, 2208, 2518, 2752, 2933, 3215
Ama87I CYCGRG 1 cut(s) 584
Aor13HI TCCGGA 1 cut(s) 1861
AoxI GGCC 5 cut(s) 661, 2463, 2734, 2948, 3275
ApoI RAATTY 8 cut(s) 566, 1423, 1591, 2140, 2146, 2377, 2765, 3204
Asp700I GAANNNNTTC 1 cut(s) 1515
AspLEI GCGC 2 cut(s) 829, 1696
AspS9I GGNCC 8 cut(s) 352, 1280, 1436, 1493, 1652, 1987, 2463, 2735
AsuHPI GGTGA 9 cut(s) 515, 953, 1046, 1475, 2016, 2053, 2317, 2687, 2978
AsuII TTCGAA 3 cut(s) 146, 2144, 2222
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 6 cut(s) 352, 1280, 1436, 1493, 1652, 1987
BaeGI GKGCMC 1 cut(s) 1881
BanI GGYRCC 2 cut(s) 819, 3040
BanII GRGCYC 2 cut(s) 1682, 3259
BauI CACGAG 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 2298
BceAI ACGGC 2 cut(s) 1064, 2963
BciT130I CCWGG 5 cut(s) 817, 1053, 2028, 2400, 3034
BclI TGATCA 2 cut(s) 61, 2838
BcoDI GTCTC 7 cut(s) 236, 373, 1208, 1292, 1638, 2397, 2620
BfaI CTAG 2 cut(s) 444, 2288
BfmI CTRYAG 1 cut(s) 1563
BlpI GCTNAGC 1 cut(s) 2154
Bme1390I CCNGG 5 cut(s) 817, 1053, 2028, 2400, 3034
Bme18I GGWCC 6 cut(s) 352, 1280, 1436, 1493, 1652, 1987
BmeT110I CYCGRG 1 cut(s) 584
BmgBI CACGTC 2 cut(s) 1462, 1763
BmgT120I GGNCC 8 cut(s) 352, 1280, 1436, 1493, 1652, 1987, 2463, 2735
BmiI GGNNCC 4 cut(s) 821, 1989, 3042, 3258
BmrFI CCNGG 5 cut(s) 817, 1053, 2028, 2400, 3034
BmsI GCATC 8 cut(s) 349, 478, 526, 721, 1225, 1718, 2701, 2842
BpmI CTGGAG 5 cut(s) 1398, 1920, 2049, 2421, 3030
Bpu10I CCTNAGC 5 cut(s) 449, 2298, 3045, 3261, 3305
Bpu1102I GCTNAGC 1 cut(s) 2154
Bpu14I TTCGAA 3 cut(s) 146, 2144, 2222
BpuEI CTTGAG 6 cut(s) 46, 314, 674, 704, 2150, 2537
BsaAI YACGTR 1 cut(s) 1261
BsaBI GATNNNNATC 1 cut(s) 1455
BsaI GGTCTC 2 cut(s) 1638, 2620
BsaJI CCNNGG 7 cut(s) 693, 791, 815, 2466, 2484, 3032, 3212
BsaWI WCCGGW 3 cut(s) 604, 1861, 2254
BsaXI ACNNNNNCTCC 8 cut(s) 23, 29, 53, 59, 1979, 2009, 3165, 3195
Bse118I RCCGGY 1 cut(s) 1654
Bse1I ACTGG 4 cut(s) 1282, 1654, 1775, 2610
Bse3DI GCAATG 3 cut(s) 1554, 2687, 3330
Bse8I GATNNNNATC 1 cut(s) 1455
BseAI TCCGGA 1 cut(s) 1861
BseBI CCWGG 5 cut(s) 817, 1053, 2028, 2400, 3034
BseDI CCNNGG 7 cut(s) 693, 791, 815, 2466, 2484, 3032, 3212
BseGI GGATG 6 cut(s) 469, 1456, 1709, 2857, 2871, 3042
BseJI GATNNNNATC 1 cut(s) 1455
BseMI GCAATG 3 cut(s) 1554, 2687, 3330
BseNI ACTGG 4 cut(s) 1282, 1654, 1775, 2610
BseRI GAGGAG 5 cut(s) 252, 538, 1626, 2315, 2534
BseSI GKGCMC 1 cut(s) 1881
BseYI CCCAGC 3 cut(s) 1382, 1486, 2621
BsgI GTGCAG 2 cut(s) 339, 861
Bsh1236I CGCG 3 cut(s) 163, 247, 1944
BshFI GGCC 5 cut(s) 663, 2465, 2736, 2950, 3277
BshNI GGYRCC 2 cut(s) 819, 3040
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 5 cut(s) 605, 1348, 1655, 1862, 2255
BslFI GGGAC 3 cut(s) 121, 574, 2557
BsmAI GTCTC 7 cut(s) 236, 373, 1208, 1292, 1638, 2397, 2620
BsmBI CGTCTC 1 cut(s) 236
BsmFI GGGAC 3 cut(s) 121, 574, 2557
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 5 cut(s) 663, 2465, 2736, 2950, 3277
Bso31I GGTCTC 2 cut(s) 1638, 2620
BsoBI CYCGRG 1 cut(s) 584
Bsp119I TTCGAA 3 cut(s) 146, 2144, 2222
Bsp1286I GDGCHC 4 cut(s) 330, 1682, 1881, 3259
Bsp13I TCCGGA 1 cut(s) 1861
Bsp1407I TGTACA 1 cut(s) 1549
Bsp1720I GCTNAGC 1 cut(s) 2154
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 8 cut(s) 104, 126, 161, 205, 1191, 1942, 2654, 3057
BspANI GGCC 5 cut(s) 663, 2465, 2736, 2950, 3277
BspEI TCCGGA 1 cut(s) 1861
BspFNI CGCG 3 cut(s) 163, 247, 1944
BspHI TCATGA 2 cut(s) 1309, 1519
BspLI GGNNCC 4 cut(s) 821, 1989, 3042, 3258
BspPI GGATC 9 cut(s) 433, 745, 830, 1391, 2208, 2518, 2752, 2933, 3215
BspT104I TTCGAA 3 cut(s) 146, 2144, 2222
BspT107I GGYRCC 2 cut(s) 819, 3040
BspTNI GGTCTC 2 cut(s) 1638, 2620
BsrDI GCAATG 3 cut(s) 1554, 2687, 3330
BsrFI RCCGGY 1 cut(s) 1654
BsrGI TGTACA 1 cut(s) 1549
BsrI ACTGG 4 cut(s) 1282, 1654, 1775, 2610
BssAI RCCGGY 1 cut(s) 1654
BssECI CCNNGG 7 cut(s) 693, 791, 815, 2466, 2484, 3032, 3212
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 3 cut(s) 693, 791, 2484
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 5 cut(s) 817, 1053, 2028, 2400, 3034
Bst4CI ACNGT 9 cut(s) 671, 882, 887, 990, 1094, 1447, 1868, 2758, 2793
Bst6I CTCTTC 3 cut(s) 402, 1563, 2336
BstAUI TGTACA 1 cut(s) 1549
BstBAI YACGTR 1 cut(s) 1261
BstBI TTCGAA 3 cut(s) 146, 2144, 2222
BstC8I GCNNGC 4 cut(s) 22, 1689, 1881, 2160
BstDSI CCRYGG 2 cut(s) 693, 3212
BstENI CCTNNNNNAGG 1 cut(s) 732
BstF5I GGATG 6 cut(s) 469, 1456, 1709, 2857, 2871, 3042
BstFNI CGCG 3 cut(s) 163, 247, 1944
BstHHI GCGC 2 cut(s) 829, 1696
BstMAI GTCTC 7 cut(s) 236, 373, 1208, 1292, 1638, 2397, 2620
BstMWI GCNNNNNNNGC 5 cut(s) 826, 1379, 1693, 2159, 2852
BstNI CCWGG 5 cut(s) 817, 1053, 2028, 2400, 3034
BstNSI RCATGY 4 cut(s) 1178, 1373, 1646, 1691
BstSCI CCNGG 5 cut(s) 815, 1051, 2026, 2398, 3032
BstSFI CTRYAG 1 cut(s) 1563
BstSLI GKGCMC 1 cut(s) 1881
BstSNI TACGTA 1 cut(s) 1261
BstUI CGCG 3 cut(s) 163, 247, 1944
BstX2I RGATCY 4 cut(s) 425, 2523, 2744, 2938
BstYI RGATCY 4 cut(s) 425, 2523, 2744, 2938
BsuRI GGCC 5 cut(s) 663, 2465, 2736, 2950, 3277
BtgI CCRYGG 2 cut(s) 693, 3212
BtgZI GCGATG 2 cut(s) 447, 474
BtrI CACGTC 2 cut(s) 1462, 1763
BtsCI GGATG 6 cut(s) 469, 1456, 1709, 2857, 2871, 3042
BtsI GCAGTG 2 cut(s) 330, 1105
BtsIMutI CAGTG 3 cut(s) 330, 1105, 1782
Cac8I GCNNGC 4 cut(s) 22, 1689, 1881, 2160
CciI TCATGA 2 cut(s) 1309, 1519
CfoI GCGC 2 cut(s) 829, 1696
Cfr10I RCCGGY 1 cut(s) 1654
Cfr13I GGNCC 8 cut(s) 352, 1280, 1436, 1493, 1652, 1987, 2463, 2735
CseI GACGC 2 cut(s) 253, 3185
Csp6I GTAC 6 cut(s) 76, 883, 905, 1200, 1550, 2328
CviQI GTAC 6 cut(s) 76, 883, 905, 1200, 1550, 2328
DraI TTTAAA 2 cut(s) 552, 2907
DraIII CACNNNGTG 1 cut(s) 1885
DrdI GACNNNNNNGTC 2 cut(s) 1460, 3004
DseDI GACNNNNNNGTC 2 cut(s) 1460, 3004
Eam1104I CTCTTC 3 cut(s) 402, 1563, 2336
EarI CTCTTC 3 cut(s) 402, 1563, 2336
Eco105I TACGTA 1 cut(s) 1261
Eco130I CCWWGG 3 cut(s) 693, 791, 2484
Eco24I GRGCYC 2 cut(s) 1682, 3259
Eco31I GGTCTC 2 cut(s) 1638, 2620
Eco32I GATATC 2 cut(s) 271, 1754
Eco47I GGWCC 6 cut(s) 352, 1280, 1436, 1493, 1652, 1987
Eco57I CTGAAG 2 cut(s) 954, 2340
Eco88I CYCGRG 1 cut(s) 584
EcoNI CCTNNNNNAGG 1 cut(s) 732
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 2765
EcoRII CCWGG 5 cut(s) 815, 1051, 2026, 2398, 3032
EcoRV GATATC 2 cut(s) 271, 1754
EcoT14I CCWWGG 3 cut(s) 693, 791, 2484
EcoT22I ATGCAT 2 cut(s) 1689, 3156
EcoT38I GRGCYC 2 cut(s) 1682, 3259
ErhI CCWWGG 3 cut(s) 693, 791, 2484
Esp3I CGTCTC 1 cut(s) 236
FaqI GGGAC 3 cut(s) 121, 574, 2557
FauI CCCGC 4 cut(s) 111, 168, 212, 3050
FauNDI CATATG 1 cut(s) 973
FbaI TGATCA 2 cut(s) 61, 2838
FblI GTMKAC 1 cut(s) 2207
FokI GGATG 6 cut(s) 456, 1463, 1696, 2864, 2878, 3049
FriOI GRGCYC 2 cut(s) 1682, 3259
FspAI RTGCGCAY 1 cut(s) 828
FspBI CTAG 2 cut(s) 444, 2288
FspI TGCGCA 1 cut(s) 828
GlaI GCGC 2 cut(s) 828, 1695
GsaI CCCAGC 3 cut(s) 1386, 1490, 2625
GsuI CTGGAG 5 cut(s) 1398, 1920, 2049, 2421, 3030
HaeIII GGCC 5 cut(s) 663, 2465, 2736, 2950, 3277
HapII CCGG 5 cut(s) 605, 1348, 1655, 1862, 2255
HgaI GACGC 2 cut(s) 253, 3185
HhaI GCGC 2 cut(s) 829, 1696
Hin6I GCGC 2 cut(s) 827, 1694
HinP1I GCGC 2 cut(s) 827, 1694
HincII GTYRAC 2 cut(s) 1816, 2236
HindII GTYRAC 2 cut(s) 1816, 2236
HindIII AAGCTT 2 cut(s) 2156, 2225
HpaI GTTAAC 1 cut(s) 2236
HpaII CCGG 5 cut(s) 605, 1348, 1655, 1862, 2255
HphI GGTGA 9 cut(s) 515, 953, 1046, 1475, 2016, 2053, 2317, 2687, 2978
Hpy99I CGWCG 2 cut(s) 1085, 2829
HpyCH4III ACNGT 9 cut(s) 671, 882, 887, 990, 1094, 1447, 1868, 2758, 2793
HpyCH4IV ACGT 5 cut(s) 1260, 1461, 1762, 2058, 2644
HpyF10VI GCNNNNNNNGC 5 cut(s) 826, 1379, 1693, 2159, 2852
HpySE526I ACGT 5 cut(s) 1260, 1461, 1762, 2058, 2644
HspAI GCGC 2 cut(s) 827, 1694
Kpn2I TCCGGA 1 cut(s) 1861
Ksp22I TGATCA 2 cut(s) 61, 2838
KspAI GTTAAC 1 cut(s) 2236
LweI GCATC 8 cut(s) 349, 478, 526, 721, 1225, 1718, 2701, 2842
MaeI CTAG 2 cut(s) 444, 2288
MaeII ACGT 5 cut(s) 1260, 1461, 1762, 2058, 2644
MaeIII GTNAC 9 cut(s) 5, 391, 1055, 1415, 1462, 2192, 2758, 3065, 3073
MboII GAAGA 9 cut(s) 419, 947, 1325, 1580, 1778, 2177, 2333, 2353, 3094
MfeI CAATTG 2 cut(s) 57, 2817
MflI RGATCY 4 cut(s) 425, 2523, 2744, 2938
MhlI GDGCHC 4 cut(s) 330, 1682, 1881, 3259
MlyI GAGTC 9 cut(s) 26, 32, 38, 596, 658, 805, 1294, 1318, 2141
MmeI TCCRAC 2 cut(s) 60, 66
Mph1103I ATGCAT 2 cut(s) 1689, 3156
MroI TCCGGA 1 cut(s) 1861
MroXI GAANNNNTTC 1 cut(s) 1515
MseI TTAA 9 cut(s) 168, 291, 551, 1743, 2235, 2274, 2675, 2775, 2906
MslI CAYNNNNRTG 4 cut(s) 1293, 1641, 2700, 2846
MspA1I CMGCKG 3 cut(s) 128, 1490, 2369
MspI CCGG 5 cut(s) 605, 1348, 1655, 1862, 2255
MspR9I CCNGG 5 cut(s) 817, 1053, 2028, 2400, 3034
MunI CAATTG 2 cut(s) 57, 2817
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 5 cut(s) 817, 1053, 2028, 2400, 3034
MvnI CGCG 3 cut(s) 163, 247, 1944
MwoI GCNNNNNNNGC 5 cut(s) 826, 1379, 1693, 2159, 2852
NcoI CCATGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 973
NlaIV GGNNCC 4 cut(s) 821, 1989, 3042, 3258
NmeAIII GCCGAG 1 cut(s) 2491
NmuCI GTSAC 5 cut(s) 391, 1415, 1462, 2192, 2758
NsbI TGCGCA 1 cut(s) 828
NsiI ATGCAT 2 cut(s) 1689, 3156
NspI RCATGY 4 cut(s) 1178, 1373, 1646, 1691
NspV TTCGAA 3 cut(s) 146, 2144, 2222
PaeI GCATGC 1 cut(s) 1691
PagI TCATGA 2 cut(s) 1309, 1519
PctI GAATGC 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 1515
PfeI GAWTC 4 cut(s) 958, 1228, 2044, 2417
PflMI CCANNNNNTGG 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 2398
PleI GAGTC 9 cut(s) 26, 32, 38, 595, 657, 804, 1294, 1318, 2140
PpsI GAGTC 9 cut(s) 26, 32, 38, 595, 657, 804, 1294, 1318, 2140
Ppu21I YACGTR 1 cut(s) 1261
PpuMI RGGWCCY 1 cut(s) 352
PsiI TTATAA 1 cut(s) 2084
Psp1406I AACGTT 1 cut(s) 2644
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 5 cut(s) 815, 1051, 2026, 2398, 3032
PspFI CCCAGC 3 cut(s) 1382, 1486, 2621
PspGI CCWGG 5 cut(s) 815, 1051, 2026, 2398, 3032
PspN4I GGNNCC 4 cut(s) 821, 1989, 3042, 3258
PspPI GGNCC 8 cut(s) 352, 1280, 1436, 1493, 1652, 1987, 2463, 2735
PspPPI RGGWCCY 1 cut(s) 352
PsuI RGATCY 4 cut(s) 425, 2523, 2744, 2938
PvuII CAGCTG 2 cut(s) 1490, 2369
RsaI GTAC 6 cut(s) 77, 884, 906, 1201, 1551, 2329
RsaNI GTAC 6 cut(s) 76, 883, 905, 1200, 1550, 2328
RseI CAYNNNNRTG 4 cut(s) 1293, 1641, 2700, 2846
SaqAI TTAA 9 cut(s) 168, 291, 551, 1743, 2235, 2274, 2675, 2775, 2906
Sau96I GGNCC 8 cut(s) 352, 1280, 1436, 1493, 1652, 1987, 2463, 2735
SchI GAGTC 9 cut(s) 26, 32, 38, 596, 658, 805, 1294, 1318, 2141
ScrFI CCNGG 5 cut(s) 817, 1053, 2028, 2400, 3034
SduI GDGCHC 4 cut(s) 330, 1682, 1881, 3259
SfaNI GCATC 8 cut(s) 349, 478, 526, 721, 1225, 1718, 2701, 2842
SfcI CTRYAG 1 cut(s) 1563
SfuI TTCGAA 3 cut(s) 146, 2144, 2222
SinI GGWCC 6 cut(s) 352, 1280, 1436, 1493, 1652, 1987
SmiI ATTTAAAT 1 cut(s) 552
SmiMI CAYNNNNRTG 4 cut(s) 1293, 1641, 2700, 2846
SmlI CTYRAG 6 cut(s) 25, 329, 653, 719, 2129, 2516
SmoI CTYRAG 6 cut(s) 25, 329, 653, 719, 2129, 2516
SnaBI TACGTA 1 cut(s) 1261
SphI GCATGC 1 cut(s) 1691
SsiI CCGC 8 cut(s) 104, 126, 161, 205, 1191, 1942, 2654, 3057
SspI AATATT 3 cut(s) 1621, 1807, 2890
SspMI CTAG 2 cut(s) 444, 2288
StyD4I CCNGG 5 cut(s) 815, 1051, 2026, 2398, 3032
StyI CCWWGG 3 cut(s) 693, 791, 2484
SwaI ATTTAAAT 1 cut(s) 552
TaaI ACNGT 9 cut(s) 671, 882, 887, 990, 1094, 1447, 1868, 2758, 2793
TaiI ACGT 5 cut(s) 1263, 1464, 1765, 2061, 2647
TaqI TCGA 5 cut(s) 146, 534, 1083, 2144, 2222
TaqII GACCGA 2 cut(s) 2777, 2826
TatI WGTACW 2 cut(s) 75, 1549
TauI GCSGC 1 cut(s) 1194
TfiI GAWTC 4 cut(s) 958, 1228, 2044, 2417
Tru1I TTAA 9 cut(s) 168, 291, 551, 1743, 2235, 2274, 2675, 2775, 2906
Tru9I TTAA 9 cut(s) 168, 291, 551, 1743, 2235, 2274, 2675, 2775, 2906
TscAI CASTG 3 cut(s) 330, 1112, 1782
TseFI GTSAC 5 cut(s) 391, 1415, 1462, 2192, 2758
Tsp45I GTSAC 5 cut(s) 391, 1415, 1462, 2192, 2758
TspGWI ACGGA 2 cut(s) 215, 1987
TspRI CASTG 3 cut(s) 330, 1112, 1782
Van91I CCANNNNNTGG 1 cut(s) 215
VpaK11BI GGWCC 6 cut(s) 352, 1280, 1436, 1493, 1652, 1987
XagI CCTNNNNNAGG 1 cut(s) 732
XapI RAATTY 8 cut(s) 566, 1423, 1591, 2140, 2146, 2377, 2765, 3204
XbaI TCTAGA 1 cut(s) 2287
XceI RCATGY 4 cut(s) 1178, 1373, 1646, 1691
XmiI GTMKAC 1 cut(s) 2207
XmnI GAANNNNTTC 1 cut(s) 1515
XspI CTAG 2 cut(s) 444, 2288
Zsp2I ATGCAT 2 cut(s) 1689, 3156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.