Rh4DG111100

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005070
Physical Location & Seq
Forward (+)
17378920 .. 17385023
6104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG111100.1

Sequence Viewer

Length: 3123 bp
ATGTGTAATAAAGGAATTGCATGCTTGAGTGAGTCGGATAACCCAATTGATCAGCAGCAGCAACAGGCTGTCTACTTGATGGAGAGCCCTTCCACTACCCCTCACTTTGGCTCTAATTCCACCCATGTTTCCAGCCCTGTTTTGAATGAGGACAATCCCCCTCGTGTTAAATTTCTGTGTAGTTTCTCCGGTAGTATATTGCCTCGGCCGCAGGATGGGAGGCTCCGATATGTCGGTGGGGAGACGCGGATTGTTAGTGTTCCGCGAGACATTAGGTATGAGGAGTTGATGAGCAAGATGAGGGAGCTTTATGAAGGGGCGGCGGTGCTTAAGTACCAGCAACCGGATGAGGACCTTGACGCCCTTGTCTCGGTTGTGAATGACGATGATGTGACCAACATGATGGAAGAGTATGATAAGTTGGGGTCGGGTGATGGTTTCACCAGGCTCAGGATTTTTTTGTTTTCGTATCCCGACCAAGATGGTGGCTCCTCACACTATGACGGGGATGAAAGGGATCATGAGAGGAGGTATGTTGATGCGCTCAATAACTTAAATGACGGTTCCGAATTTAGGAAGCAGCATTTAGAGTCCCCTGTTATCACTTCAGTTGATGATCTCCATGGAGTTGAACAGTTCTTTAGTCCACTGAGTCTCGAGGGTGGCCTCCATAGCCAGAGAAACGAGATATCCATGTCTCATTATAACCTGCATCGCCTCAAAATACCTCATATAGGTTCAGGGCAACACCATCAGCCCATTAGCCAAAGGTATAACGACATGGAAGCTCCGTGGAGTCCTGCATACTATTCTCCTAGGCATCATGGGCACCTTGATCCAAGACCAATGGCAGAGTTTCCAACTTCTCCTACCTCTGCACGGTACCGTATGCCATTTACAGATGTACCTGATAAATGTTCAGATAGAATGCCTGAAGAATATGCACGGCCACCATTGAATCATCAGCCTGGATATGAACACCAACCACAGTATTCTGATAATCCTGTATGGCTTCCAAGTGGAGCTATATCTGGTGAGAAGTCAGGCTTTCCAGGTAACATTTTTCATGGAAACAATGTTCTTGAAGGAAACAGTGTCTCTGAACACTGTCGGGTATGTTTCCAAAGAAACCAACCACATTTCGAGCAACCCAACATGGTCAATGGATTTCATCAGGTTGCTAGTACATGTGCTGATTGCCGTCCAAAGTTGCACCATGGTTACACAACCGAGCAGAATAATGATTCTTCACCTCTATATAATGAGAATCAAAATCAATGGGTTCCACACCGTTTGAATAGCCGGGCTGAGGAAGCAAGATCACATGTCTCTGGAACTGGAAAATTGAACGATCACTACATTGTAGATGGTCCTGGCATGAATTTGCCTCTTGTTCATAATAATATGGTGGATGTCCATCACGTCTCTACCAATTTTGTCCATCAACGAGCTGGGTCTGAAATGGGAAATGACGTGTTTCATGACCGACCTGTGGCTGGCCCACCCCACGTACACATTTCTCCTGTAGAAGACCGTGGTGTTCCATATGGAAATCTTCCTTATGCTTACGGAGGAGATAATGTTTACCCAGTGTCTCATGGACATGCACCGGGACATGGAGTATGGAGAAATGTTCATAGCACAATGCATCCTGTGCCCCCTTACGATGCATCCAATTCAGCTCCCCAAATTAATGGTTCTGTCAATCCAGGATATCTCAGACACGAGGGTAGTCCAAGATTTTGCATTGCAGCAGACAATCAGAATCTTTGGGTTGAATCATCTCAGAAGGTGTTAGGTTTTGACGGAAGATCTGTTCCAGACTATTCTTATGGCCAAGCTTTGAACTTGAACCCTAACACACTTGCTCATGAAAATCATCATCAATTTCCTCCAGAACTCATTCAACCCACACCTGATATAGTAAGTTCTGCTACCCCTTTGGACGGCGTCACTAGAGTTATGTTGGATACAGAGAGTTTACCTAGAGAAGGAAGGGAGGTGATTGATGGAGAGAAGGTTGAGAACTCTGATATGCATGTTATAAATAAAAAAGAATTGGGTGATATAAGTGAAGTGGTGCCTCTCGAGTTAGTAAATTCAAATTGTCTCAAACTCGCAGAAGAAAGTGGTGACAAGGATCATTCTACTCCTGAAATTTCAAAGCTTTCAGCTAACAATTTGAGTTTTGTGCCTGAGTTGATTGCTTCTGTAAAAAAGGTGGCCATATATGGGGTAGAGGAGGTAAAAGCAAAAGTTGAGGAAAGTACTGATTCTCAGAAGAGTAATCAGATTGCCAATGAAGCTGCAACAAACAGCTTGGAACCAGTGAACACTCCTGGAGATGGCGAAGTGGATTCTGATAGTGAGAATCTGAACAATTCCAAAATTGAGCCAACAAAGGCCGAGGCAGAAGCTATTTCCAAGGGATTGCAGACAATAAAGAATGATGATCTTGAGGAGATCCGAGAATTAGGTTCTGGAACATATGGGGCTGTTTTTCATGGGAAGTGGAAGGGTTCAGATGTAGCAATAAAGAGAATAAAGGCCAGCTGTTTTGCTGGCCGACCTTCTGAAAGAGAACGTTTGATTGCTGATTTCTGGAAGGAGGCTTTGATACTTAGTTCATTGCATCATCCAAATGTTGTTTCTTTCTATGGTATTGTGCGTGATGGTCCTGATGGCTCTTTAGCAACTGTTACTGAATTCATGGTTAATGGATCTTTGAAACAGTTTTTGCAGAAAAAAGACAGAACAATTGATCGTCGTAAACGATTGATTATAGCTATGGATGCTGCATTCGGGATGGAGTATCTGCATGGAAAAAATATAGTACATTTTGATTTGAAGTGTGAAAATCTGTTGGTAAATATGAGAGATCCACAGCGGCCTGTATGCAAGATTGGTGATTTGGGATTGTCAAAAGTGAAACAACAGACATTAGTGTCTGGAGGTGTTCGTGGAACTTTGCCCTGGATGGCACCTGAGCTTCTTAGTGGGAAAAGTCATATGGTGACAGAGAAGATTGATGTTTACTCATTTGGGATTGTTATGTGGGAACTGCTTACTGGCGATGAACCCTATACAGATATGCATTGTGCTTCTATAATTGGTAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1040

Amino Acids

115.84

Weight (kDa)

5.57

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 72 - 155 1.2e-17 PB1 domain
Pkinase PF00069 820 - 1030 4.5e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 821 - 1036 7.5e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 705, 2045
AasI GACNNNNNNGTC 2 cut(s) 365, 2944
Acc36I ACCTGC 1 cut(s) 717
Acc65I GGTACC 1 cut(s) 882
AccB1I GGYRCC 4 cut(s) 828, 882, 2080, 2980
AccI GTMKAC 1 cut(s) 72
AccII CGCG 2 cut(s) 247, 265
AciI CCGC 6 cut(s) 209, 247, 263, 320, 323, 2887
AclI AACGTT 1 cut(s) 2584
AclWI GGATC 6 cut(s) 525, 830, 2148, 2458, 2728, 2873
AcoI YGGCCR 5 cut(s) 206, 947, 1834, 2223, 2563
AcsI RAATTY 6 cut(s) 170, 569, 1381, 2098, 2157, 2705
AcuI CTGAAG 2 cut(s) 591, 954
AcyI GRCGYC 2 cut(s) 360, 1950
AfaI GTAC 7 cut(s) 335, 884, 906, 1186, 1512, 2269, 2835
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 3 cut(s) 1187, 1324, 1473
AjiI CACGTC 2 cut(s) 1423, 1474
AjnI CCWGG 7 cut(s) 443, 967, 1051, 1372, 1708, 2338, 2972
AjuI GAANNNNNNNTTGG 2 cut(s) 2632, 2664
AloI GAACNNNNNNTCC 4 cut(s) 1062, 1094, 1800, 1832
AlwI GGATC 6 cut(s) 525, 830, 2148, 2458, 2728, 2873
Ama87I CYCGRG 2 cut(s) 656, 2087
ApeKI GCWGC 6 cut(s) 55, 58, 580, 1751, 2306, 2795
ApoI RAATTY 6 cut(s) 170, 569, 1381, 2098, 2157, 2705
AseI ATTAAT 1 cut(s) 1692
Asp700I GAANNNNTTC 1 cut(s) 1902
Asp718I GGTACC 1 cut(s) 882
AspA2I CCTAGG 1 cut(s) 815
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 4 cut(s) 352, 1370, 1499, 2675
AsuC2I CCSGG 2 cut(s) 1304, 1611
AsuHPI GGTGA 9 cut(s) 433, 443, 1046, 1242, 2014, 2075, 2144, 2918, 3025
AvaI CYCGRG 2 cut(s) 656, 2087
AvaII GGWCC 3 cut(s) 352, 1370, 2675
AvrII CCTAGG 1 cut(s) 815
BaeGI GKGCMC 2 cut(s) 831, 1659
BaeI ACNNNNGTAYC 2 cut(s) 888, 921
BalI TGGCCA 2 cut(s) 1836, 2225
BanI GGYRCC 4 cut(s) 828, 882, 2080, 2980
BanII GRGCYC 1 cut(s) 89
BauI CACGAG 2 cut(s) 162, 1724
BbsI GAAGAC 1 cut(s) 1536
BbvCI CCTCAGC 1 cut(s) 1308
BbvI GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
BceAI ACGGC 3 cut(s) 962, 1185, 1963
BciT130I CCWGG 7 cut(s) 445, 969, 1053, 1374, 1710, 2340, 2974
BciVI GTATCC 2 cut(s) 480, 1963
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 2 cut(s) 1304, 1611
BfaI CTAG 4 cut(s) 816, 1182, 1956, 1986
BfmI CTRYAG 1 cut(s) 1524
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 717
BfuI GTATCC 2 cut(s) 480, 1963
BglII AGATCT 1 cut(s) 1811
BisI GCNGC 9 cut(s) 56, 59, 209, 321, 581, 1752, 2307, 2796, 2888
BlnI CCTAGG 1 cut(s) 815
BlsI GCNGC 9 cut(s) 57, 60, 210, 322, 582, 1753, 2308, 2797, 2889
BmcAI AGTACT 1 cut(s) 2269
Bme1390I CCNGG 9 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340, 2974
Bme18I GGWCC 3 cut(s) 352, 1370, 2675
BmeT110I CYCGRG 2 cut(s) 656, 2087
BmgBI CACGTC 2 cut(s) 1423, 1474
BmgT120I GGNCC 4 cut(s) 352, 1370, 1499, 2675
BmiI GGNNCC 9 cut(s) 224, 490, 565, 830, 884, 1284, 2082, 2325, 2982
BmrFI CCNGG 9 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340, 2974
BmrI ACTGGG 1 cut(s) 1583
BmsI GCATC 8 cut(s) 529, 721, 829, 1657, 1657, 1679, 2641, 2782
BmuI ACTGGG 1 cut(s) 1583
BpiI GAAGAC 1 cut(s) 1536
BpmI CTGGAG 3 cut(s) 1878, 2361, 2970
Bpu10I CCTNAGC 3 cut(s) 449, 1308, 2985
BpuEI CTTGAG 2 cut(s) 46, 2477
BpuMI CCSGG 2 cut(s) 1304, 1611
BsaAI YACGTR 1 cut(s) 1510
BsaBI GATNNNNATC 1 cut(s) 1416
BsaHI GRCGYC 2 cut(s) 360, 1950
BsaJI CCNNGG 9 cut(s) 203, 622, 791, 815, 1216, 1534, 2406, 2424, 2972
BsaWI WCCGGW 2 cut(s) 188, 343
BsaXI ACNNNNNCTCC 4 cut(s) 1566, 1596, 2004, 2034
Bse1I ACTGG 4 cut(s) 1342, 1589, 2327, 3073
Bse3DI GCAATG 2 cut(s) 1746, 2627
Bse8I GATNNNNATC 1 cut(s) 1416
BseBI CCWGG 7 cut(s) 445, 969, 1053, 1374, 1710, 2340, 2974
BseDI CCNNGG 9 cut(s) 203, 622, 791, 815, 1216, 1534, 2406, 2424, 2972
BseJI GATNNNNATC 1 cut(s) 1416
BseMI GCAATG 2 cut(s) 1746, 2627
BseMII CTCAG 8 cut(s) 463, 641, 1299, 1732, 1799, 2187, 2291, 2976
BseNI ACTGG 4 cut(s) 1342, 1589, 2327, 3073
BseRI GAGGAG 6 cut(s) 296, 481, 541, 1587, 2255, 2474
BseSI GKGCMC 2 cut(s) 831, 1659
BseX3I CGGCCG 1 cut(s) 206
BseXI GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
BseYI CCCAGC 1 cut(s) 1451
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 2 cut(s) 247, 265
Bsh1285I CGRYCG 1 cut(s) 209
BshNI GGYRCC 4 cut(s) 828, 882, 2080, 2980
BsiEI CGRYCG 1 cut(s) 209
BsiHKCI CYCGRG 2 cut(s) 656, 2087
BsiSI CCGG 4 cut(s) 189, 344, 1303, 1610
BslFI GGGAC 2 cut(s) 577, 1626
BsmBI CGTCTC 2 cut(s) 236, 1429
BsmFI GGGAC 2 cut(s) 577, 1626
BsmI GAATGC 2 cut(s) 933, 2798
BsoBI CYCGRG 2 cut(s) 656, 2087
Bsp1286I GDGCHC 3 cut(s) 89, 831, 1659
Bsp19I CCATGG 2 cut(s) 622, 1216
BspACI CCGC 6 cut(s) 209, 247, 263, 320, 323, 2887
BspCNI CTCAG 8 cut(s) 462, 642, 1300, 1731, 1798, 2188, 2290, 2977
BspFNI CGCG 2 cut(s) 247, 265
BspHI TCATGA 3 cut(s) 520, 1480, 1870
BspLI GGNNCC 9 cut(s) 224, 490, 565, 830, 884, 1284, 2082, 2325, 2982
BspMI ACCTGC 1 cut(s) 717
BspPI GGATC 6 cut(s) 525, 830, 2148, 2458, 2728, 2873
BspT107I GGYRCC 4 cut(s) 828, 882, 2080, 2980
BspTI CTTAAG 1 cut(s) 329
BsrDI GCAATG 2 cut(s) 1746, 2627
BsrI ACTGG 4 cut(s) 1342, 1589, 2327, 3073
BssECI CCNNGG 9 cut(s) 203, 622, 791, 815, 1216, 1534, 2406, 2424, 2972
BssNI GRCGYC 2 cut(s) 360, 1950
BssSI CACGAG 2 cut(s) 162, 1724
BssT1I CCWWGG 4 cut(s) 622, 815, 1216, 2424
Bst2BI CACGAG 2 cut(s) 162, 1724
Bst2UI CCWGG 7 cut(s) 445, 969, 1053, 1374, 1710, 2340, 2974
Bst6I CTCTTC 2 cut(s) 402, 2276
BstACI GRCGYC 2 cut(s) 360, 1950
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 1654
BstBAI YACGTR 1 cut(s) 1510
BstC8I GCNNGC 4 cut(s) 22, 1498, 2551, 2563
BstDSI CCRYGG 4 cut(s) 622, 791, 1216, 1534
BstENI CCTNNNNNAGG 2 cut(s) 732, 1989
BstFNI CGCG 2 cut(s) 247, 265
BstHHI GCGC 1 cut(s) 544
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 7 cut(s) 208, 672, 826, 1313, 1654, 2303, 2792
BstNI CCWGG 7 cut(s) 445, 969, 1053, 1374, 1710, 2340, 2974
BstNSI RCATGY 5 cut(s) 24, 1191, 1328, 1607, 2042
BstSCI CCNGG 9 cut(s) 443, 967, 1051, 1302, 1372, 1609, 1708, 2338, 2972
BstSFI CTRYAG 1 cut(s) 1524
BstSLI GKGCMC 2 cut(s) 831, 1659
BstUI CGCG 2 cut(s) 247, 265
BstV1I GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
BstV2I GAAGAC 1 cut(s) 1536
BstX2I RGATCY 4 cut(s) 1811, 2463, 2720, 2878
BstXI CCANNNNNNTGG 3 cut(s) 403, 485, 1694
BstYI RGATCY 4 cut(s) 1811, 2463, 2720, 2878
BstZI CGGCCG 1 cut(s) 206
BsuI GTATCC 2 cut(s) 480, 1963
BtgI CCRYGG 4 cut(s) 622, 791, 1216, 1534
BtgZI GCGATG 2 cut(s) 698, 3087
BtrI CACGTC 2 cut(s) 1423, 1474
BtsIMutI CAGTG 5 cut(s) 647, 1099, 1105, 1596, 2334
BveI ACCTGC 1 cut(s) 717
Cac8I GCNNGC 4 cut(s) 22, 1498, 2551, 2563
CciI TCATGA 3 cut(s) 520, 1480, 1870
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 4 cut(s) 352, 1370, 1499, 2675
CseI GACGC 3 cut(s) 253, 368, 1939
Csp6I GTAC 7 cut(s) 334, 883, 905, 1185, 1511, 2268, 2834
CspCI CAANNNNNGTGG 2 cut(s) 1275, 1310
CviQI GTAC 7 cut(s) 334, 883, 905, 1185, 1511, 2268, 2834
DrdI GACNNNNNNGTC 2 cut(s) 365, 2944
DseDI GACNNNNNNGTC 2 cut(s) 365, 2944
EaeI YGGCCR 5 cut(s) 206, 947, 1834, 2223, 2563
EagI CGGCCG 1 cut(s) 206
Eam1104I CTCTTC 2 cut(s) 402, 2276
EarI CTCTTC 2 cut(s) 402, 2276
EclXI CGGCCG 1 cut(s) 206
Eco130I CCWWGG 4 cut(s) 622, 815, 1216, 2424
Eco24I GRGCYC 1 cut(s) 89
Eco32I GATATC 2 cut(s) 690, 1715
Eco47I GGWCC 3 cut(s) 352, 1370, 2675
Eco52I CGGCCG 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 591, 954
Eco88I CYCGRG 2 cut(s) 656, 2087
EcoNI CCTNNNNNAGG 2 cut(s) 732, 1989
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 2705
EcoRII CCWGG 7 cut(s) 443, 967, 1051, 1372, 1708, 2338, 2972
EcoRV GATATC 2 cut(s) 690, 1715
EcoT14I CCWWGG 4 cut(s) 622, 815, 1216, 2424
EcoT22I ATGCAT 4 cut(s) 1650, 1672, 2040, 3096
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 4 cut(s) 622, 815, 1216, 2424
Esp3I CGTCTC 2 cut(s) 236, 1429
FalI AAGNNNNNCTT 2 cut(s) 1031, 1063
FaqI GGGAC 2 cut(s) 577, 1626
FauNDI CATATG 3 cut(s) 1546, 2488, 3009
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 9 cut(s) 56, 59, 209, 321, 581, 1752, 2307, 2796, 2888
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 9 cut(s) 56, 59, 209, 321, 581, 1752, 2307, 2796, 2888
FspBI CTAG 4 cut(s) 816, 1182, 1956, 1986
GlaI GCGC 1 cut(s) 543
GluI GCNGC 9 cut(s) 56, 59, 209, 321, 581, 1752, 2307, 2796, 2888
GsaI CCCAGC 1 cut(s) 1455
GsuI CTGGAG 3 cut(s) 1878, 2361, 2970
HapII CCGG 4 cut(s) 189, 344, 1303, 1610
HgaI GACGC 3 cut(s) 253, 368, 1939
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 2 cut(s) 360, 1950
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HindIII AAGCTT 2 cut(s) 1839, 2165
HpaII CCGG 4 cut(s) 189, 344, 1303, 1610
HphI GGTGA 9 cut(s) 433, 443, 1046, 1242, 2014, 2075, 2144, 2918, 3025
Hpy166II GTNNAC 8 cut(s) 73, 647, 1513, 1585, 1982, 2332, 2771, 3034
Hpy8I GTNNAC 8 cut(s) 73, 647, 1513, 1585, 1982, 2332, 2771, 3034
Hpy99I CGWCG 1 cut(s) 2769
HpyCH4IV ACGT 4 cut(s) 1422, 1473, 1509, 2584
HpyF10VI GCNNNNNNNGC 7 cut(s) 208, 672, 826, 1313, 1654, 2303, 2792
HpySE526I ACGT 4 cut(s) 1422, 1473, 1509, 2584
Hsp92I GRCGYC 2 cut(s) 360, 1950
HspAI GCGC 1 cut(s) 542
KpnI GGTACC 1 cut(s) 886
Ksp22I TGATCA 1 cut(s) 49
LmnI GCTCC 6 cut(s) 228, 304, 494, 793, 1022, 1687
Lsp1109I GCAGC 6 cut(s) 67, 70, 592, 1763, 2293, 2782
LweI GCATC 8 cut(s) 529, 721, 829, 1657, 1657, 1679, 2641, 2782
MaeI CTAG 4 cut(s) 816, 1182, 1956, 1986
MaeII ACGT 4 cut(s) 1422, 1473, 1509, 2584
MaeIII GTNAC 7 cut(s) 391, 1055, 1220, 1951, 2132, 2698, 3013
MboII GAAGA 9 cut(s) 419, 947, 1239, 1541, 1547, 1821, 2135, 2293, 3034
MfeI CAATTG 2 cut(s) 45, 2757
MflI RGATCY 4 cut(s) 1811, 2463, 2720, 2878
MhlI GDGCHC 3 cut(s) 89, 831, 1659
MlsI TGGCCA 2 cut(s) 1836, 2225
MluNI TGGCCA 2 cut(s) 1836, 2225
MlyI GAGTC 4 cut(s) 41, 599, 661, 805
MmeI TCCRAC 3 cut(s) 15, 884, 1947
Mox20I TGGCCA 2 cut(s) 1836, 2225
Mph1103I ATGCAT 4 cut(s) 1650, 1672, 2040, 3096
MroXI GAANNNNTTC 1 cut(s) 1902
MscI TGGCCA 2 cut(s) 1836, 2225
MseI TTAA 5 cut(s) 168, 330, 554, 1692, 2715
MslI CAYNNNNRTG 2 cut(s) 1602, 2640
Msp20I TGGCCA 2 cut(s) 1836, 2225
MspA1I CMGCKG 2 cut(s) 2553, 2887
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 4 cut(s) 189, 344, 1303, 1610
MspR9I CCNGG 9 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340, 2974
MunI CAATTG 2 cut(s) 45, 2757
Mva1269I GAATGC 2 cut(s) 933, 2798
MvaI CCWGG 7 cut(s) 445, 969, 1053, 1374, 1710, 2340, 2974
MvnI CGCG 2 cut(s) 247, 265
MwoI GCNNNNNNNGC 7 cut(s) 208, 672, 826, 1313, 1654, 2303, 2792
NciI CCSGG 2 cut(s) 1304, 1611
NcoI CCATGG 2 cut(s) 622, 1216
NdeI CATATG 3 cut(s) 1546, 2488, 3009
NlaIV GGNNCC 9 cut(s) 224, 490, 565, 830, 884, 1284, 2082, 2325, 2982
NmeAIII GCCGAG 2 cut(s) 184, 2431
NmuCI GTSAC 4 cut(s) 391, 1951, 2132, 3013
NsiI ATGCAT 4 cut(s) 1650, 1672, 2040, 3096
NspI RCATGY 5 cut(s) 24, 1191, 1328, 1607, 2042
PaeI GCATGC 1 cut(s) 24
PaeR7I CTCGAG 2 cut(s) 656, 2087
PagI TCATGA 3 cut(s) 520, 1480, 1870
PciI ACATGT 2 cut(s) 1187, 1324
PcsI WCGNNNNNNNCGW 1 cut(s) 2770
PctI GAATGC 2 cut(s) 933, 2798
PdmI GAANNNNTTC 1 cut(s) 1902
PfeI GAWTC 8 cut(s) 958, 1244, 1267, 1765, 1778, 2273, 2357, 2371
PflFI GACNNNGTC 1 cut(s) 1949
PfoI TCCNGGA 2 cut(s) 1708, 2338
PkrI GCNGC 9 cut(s) 57, 60, 210, 322, 582, 1753, 2308, 2797, 2889
PleI GAGTC 4 cut(s) 40, 598, 660, 804
PpsI GAGTC 4 cut(s) 40, 598, 660, 804
Ppu21I YACGTR 1 cut(s) 1510
PpuMI RGGWCCY 1 cut(s) 352
PscI ACATGT 2 cut(s) 1187, 1324
PshBI ATTAAT 1 cut(s) 1692
PsiI TTATAA 2 cut(s) 705, 2045
Psp1406I AACGTT 1 cut(s) 2584
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 7 cut(s) 443, 967, 1051, 1372, 1708, 2338, 2972
PspFI CCCAGC 1 cut(s) 1451
PspGI CCWGG 7 cut(s) 443, 967, 1051, 1372, 1708, 2338, 2972
PspN4I GGNNCC 9 cut(s) 224, 490, 565, 830, 884, 1284, 2082, 2325, 2982
PspPI GGNCC 4 cut(s) 352, 1370, 1499, 2675
PspPPI RGGWCCY 1 cut(s) 352
PsrI GAACNNNNNNTAC 2 cut(s) 1340, 1372
PsuI RGATCY 4 cut(s) 1811, 2463, 2720, 2878
PsyI GACNNNGTC 1 cut(s) 1949
PvuII CAGCTG 1 cut(s) 2553
RsaI GTAC 7 cut(s) 335, 884, 906, 1186, 1512, 2269, 2835
RsaNI GTAC 7 cut(s) 334, 883, 905, 1185, 1511, 2268, 2834
RseI CAYNNNNRTG 2 cut(s) 1602, 2640
SaqAI TTAA 5 cut(s) 168, 330, 554, 1692, 2715
SatI GCNGC 9 cut(s) 56, 59, 209, 321, 581, 1752, 2307, 2796, 2888
Sau96I GGNCC 4 cut(s) 352, 1370, 1499, 2675
ScaI AGTACT 1 cut(s) 2269
SchI GAGTC 4 cut(s) 41, 599, 661, 805
ScrFI CCNGG 9 cut(s) 445, 969, 1053, 1304, 1374, 1611, 1710, 2340, 2974
SduI GDGCHC 3 cut(s) 89, 831, 1659
SfaNI GCATC 8 cut(s) 529, 721, 829, 1657, 1657, 1679, 2641, 2782
SfcI CTRYAG 1 cut(s) 1524
Sfr274I CTCGAG 2 cut(s) 656, 2087
SinI GGWCC 3 cut(s) 352, 1370, 2675
SlaI CTCGAG 2 cut(s) 656, 2087
SmiMI CAYNNNNRTG 2 cut(s) 1602, 2640
SmlI CTYRAG 5 cut(s) 25, 329, 656, 2087, 2456
SmoI CTYRAG 5 cut(s) 25, 329, 656, 2087, 2456
SphI GCATGC 1 cut(s) 24
SsiI CCGC 6 cut(s) 209, 247, 263, 320, 323, 2887
SspMI CTAG 4 cut(s) 816, 1182, 1956, 1986
StyD4I CCNGG 9 cut(s) 443, 967, 1051, 1302, 1372, 1609, 1708, 2338, 2972
StyI CCWWGG 4 cut(s) 622, 815, 1216, 2424
TaiI ACGT 4 cut(s) 1425, 1476, 1512, 2587
TaqI TCGA 3 cut(s) 657, 1143, 2088
TaqII GACCGA 1 cut(s) 1500
TatI WGTACW 3 cut(s) 1184, 2267, 2833
TauI GCSGC 3 cut(s) 211, 323, 2890
TfiI GAWTC 8 cut(s) 958, 1244, 1267, 1765, 1778, 2273, 2357, 2371
Tru1I TTAA 5 cut(s) 168, 330, 554, 1692, 2715
Tru9I TTAA 5 cut(s) 168, 330, 554, 1692, 2715
TscAI CASTG 5 cut(s) 654, 1099, 1112, 1596, 2334
TseFI GTSAC 4 cut(s) 391, 1951, 2132, 3013
TseI GCWGC 6 cut(s) 55, 58, 580, 1751, 2306, 2795
Tsp45I GTSAC 4 cut(s) 391, 1951, 2132, 3013
TspGWI ACGGA 3 cut(s) 780, 1584, 1821
TspRI CASTG 5 cut(s) 654, 1099, 1112, 1596, 2334
Tth111I GACNNNGTC 1 cut(s) 1949
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 3 cut(s) 352, 1370, 2675
VspI ATTAAT 1 cut(s) 1692
XagI CCTNNNNNAGG 2 cut(s) 732, 1989
XapI RAATTY 6 cut(s) 170, 569, 1381, 2098, 2157, 2705
XceI RCATGY 5 cut(s) 24, 1191, 1328, 1607, 2042
XcmI CCANNNNNNNNNTGG 1 cut(s) 1448
XhoI CTCGAG 2 cut(s) 656, 2087
XmaJI CCTAGG 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 1902
XspI CTAG 4 cut(s) 816, 1182, 1956, 1986
ZrmI AGTACT 1 cut(s) 2269
Zsp2I ATGCAT 4 cut(s) 1650, 1672, 2040, 3096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.