pycom13g22910

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005070
Physical Location & Seq
Forward (+)
20696343 .. 20699671
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g22910.3

Sequence Viewer

Length: 2742 bp
ATGTGTAACAAAGGAATTGCCTGCTTGAGCGACTCTGACTCCGAAAACCCAATTGATCATCAGGCTGTATACTTGATGGATAGCCCTTCCGCTACCCCTCACTTTGGTTCTAATTCCACCGCTGTTTCGTCCCCCAATTCGAACGCTGAAAACCCGCGTGTTAAGTTTCTGTGTAGTTTTTCGGGGAGTATATTGCCCCGCCCACAAGATGGGAAGCTCCGTTATGTGGGAGGAGAGACGCGAATTGTGAGTGTTCCACGAGATATCAAGTTTGATGAGTTGATTAACAAGATGAGGGAGCTTTATGAAGGTGCAGCAGTGCTCAAGTATCAGCAACCCGATGAGGACCTTGATGCTCTTGTCTCGGTTGTGAACGATGATGATGTGACTAACATGATGGAAGAGTATGATAAGTTGGGATCTGGCGATGGTTTCACTAGGCTCAGGATTTTTTTGTTTTCGCATCCCGACCAAGATAGCTCATCGCACTATGAGGGTGAGGAAAGGGATAATGAGAGGAGGTATGTCGATGCTCTCAATAATTTAAATGATAGCTCCGAATTCAGGAAGCAATATCCCGAGTCCCCTCTTATTAATCCGGTTGATGATCTCCATATAGCGGAACAATTTTTTAGTCCAATGAGTCTTGAGGGTGGCCTTCACAGTCAGAGAAATTGTGATATATCCATGGCTCAATACAACTTGCATCAGCTCAAGATAACTCATGCAGGATCGGGGCAACATCATCAACCGATTAGTCAGAGGTATAACGAAATGGAAGCTCCTTGGAGTCCTGCATACTATTCTCCCAGGCACCATGCGCACCTTGATCCAAGACCAATGGCTGAGTTTCCTTCCTCTCCTTCCTCTGCACGGTACCATATGCCGTTTCCAGATGTACCCGATAAATGTTTAGATAGAATGCCTGAAGAATATGCACGACAGCCATTGAATCATCAGCCTCCATATGAACATCAACCACAGTATTCTGAGAATGTTGTATGGCTTCCAAGTGGAGCAATAGGTGGTGAAAAGTCAGGATTTCCAGGTAACATTTTTCATGGAAACAATGCTGTCGAAGGAAACAGTATTTGTGAGCACTGCCAGATGACTTTCCAGAGAAACCAGCCACATTTTGAGCAACCAGTCATGGCAAATGGATTTCAACATGTTGCTAATCCAAGCGGCAAGTGTACTGCAAATAGAGAGACTTTCGTGATGAATCCAGATACAAAGTTGCACCATGAAATATACGTAAGTGAACAGAATACTGGTCCTCATCGTTTTAGTGAGACTCCAAATCATGAAAGAGGCTGGACTCCACATCCTCATTTGAATTGCCGGACTGAGGAAGCACGACCACATGCTTCTGGAGCTGGGAAATTGAATGATCCTTACATTGTAGATGGTCACATAAATTTGCCTCTTGGTCCTAATACGGTGGATGACCATCATGTCACTTCCAATTATGTTCATCACCCAGCTGGACCTGAAATGGGAAATGAAGTGTTTCACGAACGATCTGTGGCTGCTCCACCCCATGTACACATTGCTCCTGTAGAAGAGCGTGGTGTTCGTTATGGAAATTTTCCTTATCCCTATGGAGGGGATAATATGTATCGGGGGTCTCATGGACATGCACCTGGACAGGCAGTATGGAGAAATGTTCAGAGCCCAATGCATGCTGCGCCCCCTTACGAAGCATCCATTTCTGCTCCACAAGTGAATGGTTCTGTTAATGTGGGATATCTCAGACGTGAAGATAGTCCAGGGTTTTCCATTGCATTAGACAATCAAGATATTTGGGTTGACACCTCGCAGGAAATGTTAGGTTTGGAAGGAAAAACTGTTCCTCCGGACTATTCTTATGGGAACGCTGTGAAGTTGAACCCAAACCCACTCTGTCAAGAAAATCATCAAGCATATCCTCCAGACCGCATTCAACCTACACCTGATATGATAAATTATGCTATTCCTTTGGACCCCAGCGCTGGAGTTGTAAGGTTGGAGGAAAAGAGTTCACCTGGAGTAAATGAGGTGAATCTTGTGGACAAGGTGGAGAACCCTGAAACAGAGGTTATAAATCCAAACAATCATTGTGATAAAAATGGTGGTGTGGTATCTCTTGAGTCAATAAATTCGAATTTTGCTAAGCTTGCAGAAGAAAGTTGTAATGTTGGCAAAACAAGTGACGAGGATCACTCTACTCGTAAACTTTCAAAGCTTTCTGTTAACAATTTGAGTTTCATACCGGAATTGATTGCTTCTGTTAAAAAAGCTGCTCTAGAGGGGGCTGAGGAGGTGAAAACCAATGCTGAAGAAAGTACTGACCCTGAGAAGAGTAGTTCAATAGACAAAGAAGCAGCTGCAAAAAATTTGGAACAACCGAACACTCCTGGAGACAGGGAATTGGATTCTGATAGTGATAATCTAAACAATTCCAAAATTGAGCCAACAAAGGCCGAGGCAGAAGCTATTGCCAAGGGATTGCAGACAATAAGGAATGATGATCGATCGGAGATCCGAGAATTAGGTTCTGGGACTTACGGGGCTGTGTTTCATGGGAAGTGGAAGGGGTCTGATGTAGCAATAAAGAGAATAAAGTCCAGTTGTTTTGCTGGGAGACCATCAGAAAGAGAACGTTTGATTGCGGATTTCTGGAAGGAGGCTTTAATACTGAGTTCATTGCATCACCCAAATGTTGTTTCCATGTATGGTATTGTGCGCGATGGCCCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

914

Amino Acids

101.39

Weight (kDa)

5.29

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2081
AasI GACNNNNNNGTC 1 cut(s) 1454
Acc16I TGCGCA 1 cut(s) 822
Acc65I GGTACC 1 cut(s) 876
AccB1I GGYRCC 2 cut(s) 813, 876
AccB7I CCANNNNNTGG 1 cut(s) 209
AccI GTMKAC 1 cut(s) 69
AccII CGCG 3 cut(s) 157, 241, 2727
AccIII TCCGGA 1 cut(s) 1855
AciI CCGC 8 cut(s) 90, 120, 155, 199, 620, 1185, 1936, 2651
AclI AACGTT 1 cut(s) 2641
AclWI GGATC 6 cut(s) 427, 739, 824, 1385, 2205, 2515
AcsI RAATTY 6 cut(s) 560, 1417, 1585, 2137, 2143, 2374
AcuI CTGAAG 2 cut(s) 948, 2337
AfaI GTAC 5 cut(s) 878, 900, 1195, 1545, 2326
AfeI AGCGCT 1 cut(s) 1990
AflIII ACRYGT 1 cut(s) 1168
AgsI TTSAA 8 cut(s) 952, 1166, 1336, 1387, 1888, 1943, 2220, 2349
AjiI CACGTC 1 cut(s) 1757
AjnI CCWGG 6 cut(s) 809, 1045, 1642, 1768, 2023, 2395
AjuI GAANNNNNNNTTGG 2 cut(s) 2689, 2721
AloI GAACNNNNNNTCC 2 cut(s) 1833, 1865
Alw21I GWGCWC 2 cut(s) 324, 1101
Alw26I GTCTC 7 cut(s) 230, 367, 1202, 1286, 1632, 2394, 2617
AlwI GGATC 6 cut(s) 427, 739, 824, 1385, 2205, 2515
Ama87I CYCGRG 1 cut(s) 578
Aor13HI TCCGGA 1 cut(s) 1855
Aor51HI AGCGCT 1 cut(s) 1990
AoxI GGCC 3 cut(s) 655, 2460, 2731
ApeKI GCWGC 6 cut(s) 314, 1529, 1685, 2279, 2363, 2366
ApoI RAATTY 6 cut(s) 560, 1417, 1585, 2137, 2143, 2374
AseI ATTAAT 1 cut(s) 594
Asp700I GAANNNNTTC 1 cut(s) 1509
Asp718I GGTACC 1 cut(s) 876
AspLEI GCGC 4 cut(s) 823, 1690, 1991, 2727
AspS9I GGNCC 6 cut(s) 346, 1274, 1430, 1487, 1981, 2732
AsuHPI GGTGA 7 cut(s) 509, 1040, 1469, 2013, 2050, 2314, 2684
AsuII TTCGAA 2 cut(s) 140, 2141
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 5 cut(s) 346, 1274, 1430, 1487, 1981
BanI GGYRCC 2 cut(s) 813, 876
BanII GRGCYC 1 cut(s) 1676
BauI CACGAG 1 cut(s) 258
Bbv12I GWGCWC 2 cut(s) 324, 1101
BbvCI CCTCAGC 1 cut(s) 2295
BbvI GCAGC 6 cut(s) 326, 1516, 1672, 2266, 2353, 2375
BccI CCATC 8 cut(s) 70, 203, 391, 422, 1400, 1458, 2635, 2723
BceAI ACGGC 1 cut(s) 871
BciT130I CCWGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 7 cut(s) 230, 367, 1202, 1286, 1632, 2394, 2617
BfaI CTAG 2 cut(s) 438, 2285
BfmI CTRYAG 1 cut(s) 1557
BfoI RGCGCY 1 cut(s) 1992
BisI GCNGC 7 cut(s) 315, 1186, 1530, 1686, 2280, 2364, 2367
BlpI GCTNAGC 1 cut(s) 2151
BlsI GCNGC 7 cut(s) 316, 1187, 1531, 1687, 2281, 2365, 2368
BmcAI AGTACT 1 cut(s) 2326
Bme1390I CCNGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
Bme18I GGWCC 5 cut(s) 346, 1274, 1430, 1487, 1981
BmeT110I CYCGRG 1 cut(s) 578
BmgBI CACGTC 1 cut(s) 1757
BmgT120I GGNCC 6 cut(s) 346, 1274, 1430, 1487, 1981, 2732
BmiI GGNNCC 3 cut(s) 815, 878, 1983
BmrFI CCNGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
BmsI GCATC 6 cut(s) 343, 472, 520, 715, 1712, 2698
BplI GAGNNNNNCTC 2 cut(s) 2186, 2218
BpmI CTGGAG 5 cut(s) 1392, 1914, 2013, 2046, 2418
Bpu10I CCTNAGC 2 cut(s) 443, 2295
Bpu1102I GCTNAGC 1 cut(s) 2151
Bpu14I TTCGAA 2 cut(s) 140, 2141
BpuEI CTTGAG 5 cut(s) 46, 308, 668, 698, 2147
Bsa29I ATCGAT 1 cut(s) 2512
BsaAI YACGTR 1 cut(s) 1255
BsaBI GATNNNNATC 1 cut(s) 1449
BsaI GGTCTC 2 cut(s) 1632, 2617
BsaJI CCNNGG 6 cut(s) 687, 785, 809, 1769, 2463, 2481
BsaWI WCCGGW 3 cut(s) 598, 1855, 2251
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 1837, 1867
Bse1I ACTGG 3 cut(s) 1145, 1276, 2607
Bse3DI GCAATG 3 cut(s) 1548, 1779, 2684
Bse8I GATNNNNATC 1 cut(s) 1449
BseAI TCCGGA 1 cut(s) 1855
BseBI CCWGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
BseCI ATCGAT 1 cut(s) 2512
BseDI CCNNGG 6 cut(s) 687, 785, 809, 1769, 2463, 2481
BseGI GGATG 4 cut(s) 463, 1324, 1450, 1703
BseJI GATNNNNATC 1 cut(s) 1449
BseMI GCAATG 3 cut(s) 1548, 1779, 2684
BseMII CTCAG 8 cut(s) 457, 837, 981, 1338, 1765, 2286, 2325, 2669
BseNI ACTGG 3 cut(s) 1145, 1276, 2607
BseRI GAGGAG 3 cut(s) 246, 532, 2312
BseXI GCAGC 6 cut(s) 326, 1516, 1672, 2266, 2353, 2375
BseYI CCCAGC 4 cut(s) 1376, 1480, 1985, 2618
BsgI GTGCAG 2 cut(s) 333, 855
Bsh1236I CGCG 3 cut(s) 157, 241, 2727
Bsh1285I CGRYCG 1 cut(s) 2516
BshFI GGCC 3 cut(s) 657, 2462, 2733
BshNI GGYRCC 2 cut(s) 813, 876
BshVI ATCGAT 1 cut(s) 2512
BsiEI CGRYCG 1 cut(s) 2516
BsiHKAI GWGCWC 2 cut(s) 324, 1101
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 4 cut(s) 599, 1342, 1856, 2252
BslFI GGGAC 3 cut(s) 115, 568, 2554
BsmAI GTCTC 7 cut(s) 230, 367, 1202, 1286, 1632, 2394, 2617
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 3 cut(s) 115, 568, 2554
BsmI GAATGC 2 cut(s) 927, 1938
BsnI GGCC 3 cut(s) 657, 2462, 2733
Bso31I GGTCTC 2 cut(s) 1632, 2617
BsoBI CYCGRG 1 cut(s) 578
Bsp119I TTCGAA 2 cut(s) 140, 2141
Bsp1286I GDGCHC 3 cut(s) 324, 1101, 1676
Bsp13I TCCGGA 1 cut(s) 1855
Bsp1407I TGTACA 1 cut(s) 1543
Bsp1720I GCTNAGC 1 cut(s) 2151
Bsp19I CCATGG 1 cut(s) 687
BspACI CCGC 8 cut(s) 90, 120, 155, 199, 620, 1185, 1936, 2651
BspANI GGCC 3 cut(s) 657, 2462, 2733
BspCNI CTCAG 8 cut(s) 456, 838, 982, 1339, 1764, 2287, 2326, 2670
BspDI ATCGAT 1 cut(s) 2512
BspEI TCCGGA 1 cut(s) 1855
BspFNI CGCG 3 cut(s) 157, 241, 2727
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 3 cut(s) 815, 878, 1983
BspPI GGATC 6 cut(s) 427, 739, 824, 1385, 2205, 2515
BspQI GCTCTTC 1 cut(s) 1557
BspT104I TTCGAA 2 cut(s) 140, 2141
BspT107I GGYRCC 2 cut(s) 813, 876
BspTNI GGTCTC 2 cut(s) 1632, 2617
BsrDI GCAATG 3 cut(s) 1548, 1779, 2684
BsrGI TGTACA 1 cut(s) 1543
BsrI ACTGG 3 cut(s) 1145, 1276, 2607
BssECI CCNNGG 6 cut(s) 687, 785, 809, 1769, 2463, 2481
BssNAI GTATAC 1 cut(s) 70
BssSI CACGAG 1 cut(s) 258
BssT1I CCWWGG 3 cut(s) 687, 785, 2481
Bst1107I GTATAC 1 cut(s) 70
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
Bst4CI ACNGT 6 cut(s) 665, 876, 984, 1088, 1441, 1849
Bst6I CTCTTC 3 cut(s) 396, 1557, 2333
BstAUI TGTACA 1 cut(s) 1543
BstBAI YACGTR 1 cut(s) 1255
BstBI TTCGAA 2 cut(s) 140, 2141
BstC8I GCNNGC 3 cut(s) 22, 1683, 2157
BstDEI CTNAG 9 cut(s) 443, 846, 990, 1347, 1751, 2151, 2295, 2334, 2678
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 4 cut(s) 463, 1324, 1450, 1703
BstFNI CGCG 3 cut(s) 157, 241, 2727
BstH2I RGCGCY 1 cut(s) 1992
BstHHI GCGC 4 cut(s) 823, 1690, 1991, 2727
BstMAI GTCTC 7 cut(s) 230, 367, 1202, 1286, 1632, 2394, 2617
BstMCI CGRYCG 1 cut(s) 2516
BstMWI GCNNNNNNNGC 4 cut(s) 820, 1373, 1687, 2156
BstNI CCWGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
BstNSI RCATGY 4 cut(s) 1172, 1367, 1640, 1685
BstSCI CCNGG 6 cut(s) 809, 1045, 1642, 1768, 2023, 2395
BstSFI CTRYAG 1 cut(s) 1557
BstSNI TACGTA 1 cut(s) 1255
BstUI CGCG 3 cut(s) 157, 241, 2727
BstV1I GCAGC 6 cut(s) 326, 1516, 1672, 2266, 2353, 2375
BstX2I RGATCY 2 cut(s) 419, 2520
BstYI RGATCY 2 cut(s) 419, 2520
BstZ17I GTATAC 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 2512
BsuRI GGCC 3 cut(s) 657, 2462, 2733
BsuTUI ATCGAT 1 cut(s) 2512
BtgI CCRYGG 1 cut(s) 687
BtgZI GCGATG 2 cut(s) 441, 468
BtrI CACGTC 1 cut(s) 1757
BtsCI GGATG 4 cut(s) 463, 1324, 1450, 1703
BtsI GCAGTG 2 cut(s) 324, 1099
BtsIMutI CAGTG 2 cut(s) 324, 1099
Cac8I GCNNGC 3 cut(s) 22, 1683, 2157
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 4 cut(s) 823, 1690, 1991, 2727
Cfr13I GGNCC 6 cut(s) 346, 1274, 1430, 1487, 1981, 2732
ClaI ATCGAT 1 cut(s) 2512
CseI GACGC 1 cut(s) 247
Csp6I GTAC 5 cut(s) 877, 899, 1194, 1544, 2325
CviQI GTAC 5 cut(s) 877, 899, 1194, 1544, 2325
DdeI CTNAG 9 cut(s) 443, 846, 990, 1347, 1751, 2151, 2295, 2334, 2678
DraI TTTAAA 1 cut(s) 546
DrdI GACNNNNNNGTC 1 cut(s) 1454
DseDI GACNNNNNNGTC 1 cut(s) 1454
Eam1104I CTCTTC 3 cut(s) 396, 1557, 2333
EarI CTCTTC 3 cut(s) 396, 1557, 2333
Eco105I TACGTA 1 cut(s) 1255
Eco130I CCWWGG 3 cut(s) 687, 785, 2481
Eco24I GRGCYC 1 cut(s) 1676
Eco31I GGTCTC 2 cut(s) 1632, 2617
Eco32I GATATC 2 cut(s) 265, 1748
Eco47I GGWCC 5 cut(s) 346, 1274, 1430, 1487, 1981
Eco47III AGCGCT 1 cut(s) 1990
Eco57I CTGAAG 2 cut(s) 948, 2337
Eco88I CYCGRG 1 cut(s) 578
EcoO109I RGGNCCY 1 cut(s) 346
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 6 cut(s) 809, 1045, 1642, 1768, 2023, 2395
EcoRV GATATC 2 cut(s) 265, 1748
EcoT14I CCWWGG 3 cut(s) 687, 785, 2481
EcoT22I ATGCAT 1 cut(s) 1683
EcoT38I GRGCYC 1 cut(s) 1676
ErhI CCWWGG 3 cut(s) 687, 785, 2481
Esp3I CGTCTC 1 cut(s) 230
FaqI GGGAC 3 cut(s) 115, 568, 2554
FauI CCCGC 2 cut(s) 162, 206
FauNDI CATATG 2 cut(s) 882, 967
FbaI TGATCA 1 cut(s) 55
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 7 cut(s) 315, 1186, 1530, 1686, 2280, 2364, 2367
FokI GGATG 4 cut(s) 450, 1311, 1457, 1690
FriOI GRGCYC 1 cut(s) 1676
Fsp4HI GCNGC 7 cut(s) 315, 1186, 1530, 1686, 2280, 2364, 2367
FspAI RTGCGCAY 1 cut(s) 822
FspBI CTAG 2 cut(s) 438, 2285
FspI TGCGCA 1 cut(s) 822
GlaI GCGC 4 cut(s) 822, 1689, 1990, 2726
GluI GCNGC 7 cut(s) 315, 1186, 1530, 1686, 2280, 2364, 2367
GsaI CCCAGC 4 cut(s) 1380, 1484, 1989, 2622
GsuI CTGGAG 5 cut(s) 1392, 1914, 2013, 2046, 2418
HaeII RGCGCY 1 cut(s) 1992
HaeIII GGCC 3 cut(s) 657, 2462, 2733
HapII CCGG 4 cut(s) 599, 1342, 1856, 2252
HgaI GACGC 1 cut(s) 247
HhaI GCGC 4 cut(s) 823, 1690, 1991, 2727
Hin6I GCGC 4 cut(s) 821, 1688, 1989, 2725
HinP1I GCGC 4 cut(s) 821, 1688, 1989, 2725
HincII GTYRAC 2 cut(s) 1810, 2233
HindII GTYRAC 2 cut(s) 1810, 2233
HindIII AAGCTT 2 cut(s) 2153, 2222
HpaI GTTAAC 1 cut(s) 2233
HpaII CCGG 4 cut(s) 599, 1342, 1856, 2252
HphI GGTGA 7 cut(s) 509, 1040, 1469, 2013, 2050, 2314, 2684
HpyAV CCTTC 9 cut(s) 96, 302, 668, 864, 873, 1073, 1832, 2566, 2656
HpyCH4III ACNGT 6 cut(s) 665, 876, 984, 1088, 1441, 1849
HpyCH4IV ACGT 3 cut(s) 1254, 1756, 2641
HpyF10VI GCNNNNNNNGC 4 cut(s) 820, 1373, 1687, 2156
HpyF3I CTNAG 9 cut(s) 443, 846, 990, 1347, 1751, 2151, 2295, 2334, 2678
HpySE526I ACGT 3 cut(s) 1254, 1756, 2641
HspAI GCGC 4 cut(s) 821, 1688, 1989, 2725
Kpn2I TCCGGA 1 cut(s) 1855
KpnI GGTACC 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 55
KspAI GTTAAC 1 cut(s) 2233
LguI GCTCTTC 1 cut(s) 1557
LmnI GCTCC 9 cut(s) 222, 298, 560, 787, 1016, 1373, 1537, 1558, 1720
Lsp1109I GCAGC 6 cut(s) 326, 1516, 1672, 2266, 2353, 2375
LweI GCATC 6 cut(s) 343, 472, 520, 715, 1712, 2698
MaeI CTAG 2 cut(s) 438, 2285
MaeII ACGT 3 cut(s) 1254, 1756, 2641
MaeIII GTNAC 6 cut(s) 5, 385, 1049, 1409, 1456, 2189
MboII GAAGA 7 cut(s) 413, 941, 1574, 1772, 2174, 2330, 2350
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 2 cut(s) 419, 2520
MhlI GDGCHC 3 cut(s) 324, 1101, 1676
MlyI GAGTC 8 cut(s) 26, 32, 590, 652, 799, 1288, 1312, 2138
MmeI TCCRAC 1 cut(s) 1986
Mph1103I ATGCAT 1 cut(s) 1683
MroI TCCGGA 1 cut(s) 1855
MroXI GAANNNNTTC 1 cut(s) 1509
MseI TTAA 8 cut(s) 162, 285, 545, 594, 1737, 2232, 2271, 2672
MslI CAYNNNNRTG 2 cut(s) 1635, 2697
MspA1I CMGCKG 3 cut(s) 122, 1484, 2366
MspI CCGG 4 cut(s) 599, 1342, 1856, 2252
MspR9I CCNGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 2 cut(s) 927, 1938
MvaI CCWGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
MvnI CGCG 3 cut(s) 157, 241, 2727
MwoI GCNNNNNNNGC 4 cut(s) 820, 1373, 1687, 2156
NcoI CCATGG 1 cut(s) 687
NdeI CATATG 2 cut(s) 882, 967
NlaIV GGNNCC 3 cut(s) 815, 878, 1983
NmeAIII GCCGAG 1 cut(s) 2488
NmuCI GTSAC 4 cut(s) 385, 1409, 1456, 2189
NsbI TGCGCA 1 cut(s) 822
NsiI ATGCAT 1 cut(s) 1683
NspI RCATGY 4 cut(s) 1172, 1367, 1640, 1685
NspV TTCGAA 2 cut(s) 140, 2141
PaeI GCATGC 1 cut(s) 1685
PagI TCATGA 1 cut(s) 1303
PciI ACATGT 1 cut(s) 1168
PciSI GCTCTTC 1 cut(s) 1557
PctI GAATGC 2 cut(s) 927, 1938
PdmI GAANNNNTTC 1 cut(s) 1509
PfeI GAWTC 4 cut(s) 952, 1222, 2041, 2414
PflMI CCANNNNNTGG 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 2395
PkrI GCNGC 7 cut(s) 316, 1187, 1531, 1687, 2281, 2365, 2368
Ple19I CGATCG 1 cut(s) 2516
PleI GAGTC 8 cut(s) 26, 32, 589, 651, 798, 1288, 1312, 2137
PpsI GAGTC 8 cut(s) 26, 32, 589, 651, 798, 1288, 1312, 2137
Ppu21I YACGTR 1 cut(s) 1255
PpuMI RGGWCCY 1 cut(s) 346
PscI ACATGT 1 cut(s) 1168
PshBI ATTAAT 1 cut(s) 594
PsiI TTATAA 1 cut(s) 2081
Psp1406I AACGTT 1 cut(s) 2641
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 6 cut(s) 809, 1045, 1642, 1768, 2023, 2395
PspFI CCCAGC 4 cut(s) 1376, 1480, 1985, 2618
PspGI CCWGG 6 cut(s) 809, 1045, 1642, 1768, 2023, 2395
PspN4I GGNNCC 3 cut(s) 815, 878, 1983
PspPI GGNCC 6 cut(s) 346, 1274, 1430, 1487, 1981, 2732
PspPPI RGGWCCY 1 cut(s) 346
PsuI RGATCY 2 cut(s) 419, 2520
PvuI CGATCG 1 cut(s) 2516
PvuII CAGCTG 2 cut(s) 1484, 2366
RsaI GTAC 5 cut(s) 878, 900, 1195, 1545, 2326
RsaNI GTAC 5 cut(s) 877, 899, 1194, 1544, 2325
RseI CAYNNNNRTG 2 cut(s) 1635, 2697
SapI GCTCTTC 1 cut(s) 1557
SaqAI TTAA 8 cut(s) 162, 285, 545, 594, 1737, 2232, 2271, 2672
SatI GCNGC 7 cut(s) 315, 1186, 1530, 1686, 2280, 2364, 2367
Sau96I GGNCC 6 cut(s) 346, 1274, 1430, 1487, 1981, 2732
ScaI AGTACT 1 cut(s) 2326
SchI GAGTC 8 cut(s) 26, 32, 590, 652, 799, 1288, 1312, 2138
ScrFI CCNGG 6 cut(s) 811, 1047, 1644, 1770, 2025, 2397
SduI GDGCHC 3 cut(s) 324, 1101, 1676
SfaNI GCATC 6 cut(s) 343, 472, 520, 715, 1712, 2698
SfcI CTRYAG 1 cut(s) 1557
SfuI TTCGAA 2 cut(s) 140, 2141
SinI GGWCC 5 cut(s) 346, 1274, 1430, 1487, 1981
SmiI ATTTAAAT 1 cut(s) 546
SmiMI CAYNNNNRTG 2 cut(s) 1635, 2697
SmlI CTYRAG 5 cut(s) 25, 323, 647, 713, 2126
SmoI CTYRAG 5 cut(s) 25, 323, 647, 713, 2126
SnaBI TACGTA 1 cut(s) 1255
SphI GCATGC 1 cut(s) 1685
SsiI CCGC 8 cut(s) 90, 120, 155, 199, 620, 1185, 1936, 2651
SspMI CTAG 2 cut(s) 438, 2285
StyD4I CCNGG 6 cut(s) 809, 1045, 1642, 1768, 2023, 2395
StyI CCWWGG 3 cut(s) 687, 785, 2481
SwaI ATTTAAAT 1 cut(s) 546
TaaI ACNGT 6 cut(s) 665, 876, 984, 1088, 1441, 1849
TaiI ACGT 3 cut(s) 1257, 1759, 2644
TaqI TCGA 5 cut(s) 140, 528, 1077, 2141, 2512
TatI WGTACW 3 cut(s) 1193, 1543, 2324
TauI GCSGC 1 cut(s) 1188
TfiI GAWTC 4 cut(s) 952, 1222, 2041, 2414
Tru1I TTAA 8 cut(s) 162, 285, 545, 594, 1737, 2232, 2271, 2672
Tru9I TTAA 8 cut(s) 162, 285, 545, 594, 1737, 2232, 2271, 2672
TscAI CASTG 2 cut(s) 324, 1106
TseFI GTSAC 4 cut(s) 385, 1409, 1456, 2189
TseI GCWGC 6 cut(s) 314, 1529, 1685, 2279, 2363, 2366
Tsp45I GTSAC 4 cut(s) 385, 1409, 1456, 2189
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 2 cut(s) 324, 1106
Van91I CCANNNNNTGG 1 cut(s) 209
VpaK11BI GGWCC 5 cut(s) 346, 1274, 1430, 1487, 1981
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 6 cut(s) 560, 1417, 1585, 2137, 2143, 2374
XbaI TCTAGA 1 cut(s) 2284
XceI RCATGY 4 cut(s) 1172, 1367, 1640, 1685
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 1509
XspI CTAG 2 cut(s) 438, 2285
ZrmI AGTACT 1 cut(s) 2326
Zsp2I ATGCAT 1 cut(s) 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.