FvH4_4g10270

FLX-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002508
Physical Location & Seq
Reverse (-)
13597937 .. 13601283
3347 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10270.t3

Sequence Viewer

Length: 999 bp
ATGGCTGGGCGACGACATATTGCATCATCCTATGACAGACGCCCTGTGCAGTCTCCAGGGATGATGCGGCATGGGTCATTGCCTGGCTACCGGTTATTAGAGTCACTACCCCGTCCTGAACTATTGGAAAACAAAATTGCATATCAAGCAGCAGAGATAAAACAACTTGCAGGAGACAATCATAGATTGGCAACTTCTCAGGTGGATTTGAGGCAGGAGCTTCTAGCTGCAGAGGAGGAAGTTCAAACTGTCAAGGCACACATGAGAAGTATCCAGACTGAAAGCGACATCCAGATCCGGGCGATGCTTGATAAAATTGCCAAAAAGGAAGCAGAAGTTGTAGCCTGTGAGGTTGTGAGGAAGGAGCTGCAAAAGGCTCATACGGAAGCACACAGCTTGGTCGAAACACGGAAAGAGCTGAGAATTCAAATTCGACAGGCTACGGATGAGTTGCAGAAATCCCGTGTTGAGGTTAAGAAACTGCCAGATCTGCATGGTGAACTTGACAGTTTGAGGCAAGAACACCAGAAGCTGCGCGCTACTTTTGAATATGAAAAAAGTAGAAACATAGAACTAGTGGAGCAAATGAAAGCAATGGAGAAGAATCTAATGGGCATGGCTGGAGAAGTAGAAAGGTTGTGTGATGAGGTCCTGAATGCAGAGAAGAGAGCACAAGCCCCAAAGCCACCTAATGGTGGTTACATGAATCCAGATTCTTATTACACTGCTACCATGCCAGGTGCTAGTGCATATTTTGATGGTTATGGAAGGCCTTATGTTTCGGTGGGTAATAGGCCACTGGGAGAGGGAATAATTTACTTTGCCAGCAGCAGCAGCAAGCAGCATGCTGCTGGTATCCCAACTGCTGGGGATGGTGCTATCTGGGGAGCAGCATATAATCCCTTGCCTGACACTGCTTCTGGTGGTGCTACTGTCCCTACCACTGGGAACAGTGCTGTTTGGGGAGGAGCATATGATTCAGCACTTGCTCGGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

333

Amino Acids

36.67

Weight (kDa)

6.78

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LAMB4 PF24999 132 - 181 5.4e-06 Laminin subunit beta-4-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 692, 866
AccII CGCG 1 cut(s) 537
AciI CCGC 1 cut(s) 67
AclWI GGATC 1 cut(s) 289
AcsI RAATTY 2 cut(s) 423, 429
AcyI GRCGYC 1 cut(s) 40
AfiI CCNNNNNNNGG 6 cut(s) 298, 469, 692, 695, 866, 944
AgeI ACCGGT 1 cut(s) 90
AgsI TTSAA 3 cut(s) 245, 428, 548
AhlI ACTAGT 1 cut(s) 574
AjnI CCWGG 3 cut(s) 55, 82, 736
AluBI AGCT 6 cut(s) 220, 227, 367, 396, 418, 532
AluI AGCT 6 cut(s) 220, 227, 367, 396, 418, 532
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 2 cut(s) 57, 168
AlwI GGATC 1 cut(s) 289
AlwNI CAGNNNCTG 1 cut(s) 532
AoxI GGCC 2 cut(s) 770, 794
ApoI RAATTY 2 cut(s) 423, 429
AsiGI ACCGGT 1 cut(s) 90
AspLEI GCGC 2 cut(s) 537, 539
AspS9I GGNCC 1 cut(s) 649
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 509
AvaII GGWCC 1 cut(s) 649
Bbv12I GWGCWC 1 cut(s) 673
BccI CCATC 2 cut(s) 752, 866
BciT130I CCWGG 3 cut(s) 57, 84, 738
BciVI GTATCC 2 cut(s) 281, 866
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 57, 168
BcuI ACTAGT 1 cut(s) 574
BfaI CTAG 3 cut(s) 224, 575, 744
BfmI CTRYAG 1 cut(s) 228
BfuI GTATCC 2 cut(s) 281, 866
BglII AGATCT 1 cut(s) 487
Bme1390I CCNGG 4 cut(s) 57, 84, 299, 738
Bme18I GGWCC 1 cut(s) 649
BmgT120I GGNCC 1 cut(s) 649
BmrFI CCNGG 4 cut(s) 57, 84, 299, 738
BmrI ACTGGG 2 cut(s) 809, 954
BmsI GCATC 3 cut(s) 32, 54, 294
BmuI ACTGGG 2 cut(s) 809, 954
BpmI CTGGAG 2 cut(s) 39, 642
BpuMI CCSGG 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 56
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 6 cut(s) 298, 469, 692, 695, 866, 944
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 2 cut(s) 804, 949
Bse3DI GCAATG 2 cut(s) 77, 600
BseBI CCWGG 3 cut(s) 57, 84, 738
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 5 cut(s) 26, 66, 288, 451, 877
BseLI CCNNNNNNNGG 6 cut(s) 298, 469, 692, 695, 866, 944
BseMI GCAATG 2 cut(s) 77, 600
BseMII CTCAG 2 cut(s) 212, 410
BseNI ACTGG 2 cut(s) 804, 949
BsePI GCGCGC 1 cut(s) 535
BseRI GAGGAG 2 cut(s) 248, 981
BseYI CCCAGC 2 cut(s) 5, 866
BsgI GTGCAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 2 cut(s) 772, 796
BshTI ACCGGT 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 673
BsiSI CCGG 2 cut(s) 91, 298
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 6 cut(s) 298, 469, 692, 695, 866, 944
BsmAI GTCTC 2 cut(s) 57, 168
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 2 cut(s) 772, 796
Bsp1286I GDGCHC 1 cut(s) 673
Bsp143I GATC 2 cut(s) 294, 487
BspACI CCGC 1 cut(s) 67
BspANI GGCC 2 cut(s) 772, 796
BspCNI CTCAG 2 cut(s) 211, 411
BspFNI CGCG 1 cut(s) 537
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 1 cut(s) 289
BsrDI GCAATG 2 cut(s) 77, 600
BsrFI RCCGGY 1 cut(s) 90
BsrI ACTGG 2 cut(s) 804, 949
BssAI RCCGGY 1 cut(s) 90
BssECI CCNNGG 1 cut(s) 56
BssHII GCGCGC 1 cut(s) 535
BssMI GATC 2 cut(s) 294, 487
BssNI GRCGYC 1 cut(s) 40
Bst2UI CCWGG 3 cut(s) 57, 84, 738
Bst4CI ACNGT 4 cut(s) 250, 509, 934, 953
Bst6I CTCTTC 1 cut(s) 659
BstACI GRCGYC 1 cut(s) 40
BstC8I GCNNGC 4 cut(s) 537, 826, 839, 846
BstDEI CTNAG 2 cut(s) 198, 419
BstF5I GGATG 5 cut(s) 26, 66, 288, 451, 877
BstFNI CGCG 1 cut(s) 537
BstHHI GCGC 2 cut(s) 537, 539
BstKTI GATC 2 cut(s) 297, 490
BstMAI GTCTC 2 cut(s) 57, 168
BstMBI GATC 2 cut(s) 294, 487
BstMWI GCNNNNNNNGC 3 cut(s) 146, 490, 834
BstNI CCWGG 3 cut(s) 57, 84, 738
BstNSI RCATGY 1 cut(s) 848
BstSCI CCNGG 4 cut(s) 55, 82, 297, 736
BstSFI CTRYAG 1 cut(s) 228
BstUI CGCG 1 cut(s) 537
BstX2I RGATCY 2 cut(s) 294, 487
BstYI RGATCY 2 cut(s) 294, 487
BsuI GTATCC 2 cut(s) 281, 866
BsuRI GGCC 2 cut(s) 772, 796
BtgZI GCGATG 1 cut(s) 317
BtsCI GGATG 5 cut(s) 26, 66, 288, 451, 877
BtsI GCAGTG 2 cut(s) 723, 912
BtsIMutI CAGTG 5 cut(s) 723, 797, 912, 942, 958
Cac8I GCNNGC 4 cut(s) 537, 826, 839, 846
CaiI CAGNNNCTG 1 cut(s) 532
CfoI GCGC 2 cut(s) 537, 539
Cfr10I RCCGGY 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 649
CseI GACGC 1 cut(s) 48
CspAI ACCGGT 1 cut(s) 90
CviAII CATG 7 cut(s) 71, 262, 494, 616, 703, 733, 845
DdeI CTNAG 2 cut(s) 198, 419
DpnI GATC 2 cut(s) 296, 489
DpnII GATC 2 cut(s) 294, 487
Eam1104I CTCTTC 1 cut(s) 659
EarI CTCTTC 1 cut(s) 659
Eco147I AGGCCT 1 cut(s) 772
Eco47I GGWCC 1 cut(s) 649
EcoO109I RGGNCCY 1 cut(s) 649
EcoRI GAATTC 1 cut(s) 423
EcoRII CCWGG 3 cut(s) 55, 82, 736
FaeI CATG 7 cut(s) 74, 265, 497, 619, 706, 736, 848
FaqI GGGAC 1 cut(s) 920
FatI CATG 7 cut(s) 70, 261, 493, 615, 702, 732, 844
FauNDI CATATG 1 cut(s) 973
FokI GGATG 5 cut(s) 13, 73, 275, 458, 884
FspBI CTAG 3 cut(s) 224, 575, 744
GlaI GCGC 2 cut(s) 536, 538
GsaI CCCAGC 2 cut(s) 9, 870
GsuI CTGGAG 2 cut(s) 39, 642
HaeIII GGCC 2 cut(s) 772, 796
HapII CCGG 2 cut(s) 91, 298
HgaI GACGC 1 cut(s) 48
HhaI GCGC 2 cut(s) 537, 539
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 7 cut(s) 74, 265, 497, 619, 706, 736, 848
Hin6I GCGC 2 cut(s) 535, 537
HinP1I GCGC 2 cut(s) 535, 537
HinfI GANTC 5 cut(s) 101, 604, 706, 713, 977
HpaII CCGG 2 cut(s) 91, 298
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 1 cut(s) 500
Hpy188III TCNNGA 5 cut(s) 116, 274, 292, 652, 710
Hpy8I GTNNAC 1 cut(s) 500
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 2 cut(s) 355, 762
HpyCH4III ACNGT 4 cut(s) 250, 509, 934, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 490, 834
HpyF3I CTNAG 2 cut(s) 198, 419
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 7 cut(s) 74, 265, 497, 619, 706, 736, 848
HspAI GCGC 2 cut(s) 535, 537
Kzo9I GATC 2 cut(s) 294, 487
LmnI GCTCC 5 cut(s) 217, 364, 580, 887, 968
LweI GCATC 3 cut(s) 32, 54, 294
MaeI CTAG 3 cut(s) 224, 575, 744
MaeIII GTNAC 2 cut(s) 102, 698
MalI GATC 2 cut(s) 296, 489
MboI GATC 2 cut(s) 294, 487
MboII GAAGA 2 cut(s) 613, 676
MflI RGATCY 2 cut(s) 294, 487
MhlI GDGCHC 1 cut(s) 673
MluCI AATT 5 cut(s) 135, 315, 423, 429, 813
MlyI GAGTC 1 cut(s) 110
MseI TTAA 1 cut(s) 474
MspI CCGG 2 cut(s) 91, 298
MspR9I CCNGG 4 cut(s) 57, 84, 299, 738
Mva1269I GAATGC 1 cut(s) 661
MvaI CCWGG 3 cut(s) 57, 84, 738
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 3 cut(s) 146, 490, 834
NciI CCSGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 973
NdeII GATC 2 cut(s) 294, 487
NlaIII CATG 7 cut(s) 74, 265, 497, 619, 706, 736, 848
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 1 cut(s) 102
NspI RCATGY 1 cut(s) 848
PaeI GCATGC 1 cut(s) 848
PauI GCGCGC 1 cut(s) 535
PceI AGGCCT 1 cut(s) 772
PctI GAATGC 1 cut(s) 661
PfeI GAWTC 4 cut(s) 604, 706, 713, 977
PflMI CCANNNNNTGG 2 cut(s) 692, 866
PinAI ACCGGT 1 cut(s) 90
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PpuMI RGGWCCY 1 cut(s) 649
Psp5II RGGWCCY 1 cut(s) 649
Psp6I CCWGG 3 cut(s) 55, 82, 736
PspFI CCCAGC 2 cut(s) 5, 866
PspGI CCWGG 3 cut(s) 55, 82, 736
PspPI GGNCC 1 cut(s) 649
PspPPI RGGWCCY 1 cut(s) 649
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 532
PsuI RGATCY 2 cut(s) 294, 487
PteI GCGCGC 1 cut(s) 535
SaqAI TTAA 1 cut(s) 474
Sau3AI GATC 2 cut(s) 294, 487
Sau96I GGNCC 1 cut(s) 649
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 4 cut(s) 57, 84, 299, 738
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 3 cut(s) 32, 54, 294
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 649
SpeI ACTAGT 1 cut(s) 574
SphI GCATGC 1 cut(s) 848
Sse9I AATT 5 cut(s) 135, 315, 423, 429, 813
SseBI AGGCCT 1 cut(s) 772
SsiI CCGC 1 cut(s) 67
SspMI CTAG 3 cut(s) 224, 575, 744
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 4 cut(s) 55, 82, 297, 736
TaaI ACNGT 4 cut(s) 250, 509, 934, 953
TaqI TCGA 2 cut(s) 402, 433
TasI AATT 5 cut(s) 135, 315, 423, 429, 813
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 4 cut(s) 604, 706, 713, 977
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 5 cut(s) 730, 804, 919, 949, 958
TseFI GTSAC 1 cut(s) 102
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 3 cut(s) 567, 602, 719
TspGWI ACGGA 3 cut(s) 398, 424, 458
TspRI CASTG 5 cut(s) 730, 804, 919, 949, 958
Van91I CCANNNNNTGG 2 cut(s) 692, 866
VpaK11BI GGWCC 1 cut(s) 649
XapI RAATTY 2 cut(s) 423, 429
XceI RCATGY 1 cut(s) 848
XspI CTAG 3 cut(s) 224, 575, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.