pycom13g28830

FLX-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002508
Physical Location & Seq
Forward (+)
24922788 .. 24924219
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g28830.2

Sequence Viewer

Length: 888 bp
ATGTTGGGGAGACGAAAAGTACCATCATCTTTTGAGCAACGTCCCATTCAATCTCGAGGGATGATGCAACATGGTCCATTGCCTGGGTTACGGCCTACTATTGGCCACCGATCATTTGAGTCACTACCCCGTCCTGAACTATTGGAAAATAAAACTGCAGATCAAGCAGCAGAGATAAAGCAACTGGGTGACATTCAGACCCTGGTTTTTCTTGAGAAGATTGCAAAATGGGAAGCAGACCTAAAGGCTGGTGAGGGTGTGAAGAAGGAATTGCAAAAGGCTCACATGGAGGCACAGAGCTTGTTTATAACCCGACAAGAGCTGGCCATTCAAATTCAACAGGCCAAAGAGGAATTGCAGAAAACTCGACTTGATGTTCAGAAACTACCATATTTGAATGTTGAACTTGACACCTTGAGGCAAGAACACCAGAGGCTGCATCACATATACAAATTGACTTATTCCCAATCTGTTCAGCTATTGAGCGGTTCCATATTTTCATTTCAATGTGTGTTCCTTCCTTCCAAATCAGATCTTATTAAAGACTCAACCAAATTAGCTGAACGATTTCAACAAACTATATTTTCTCGATTAAAGGTCACAATTCCTATATTTCGTTTTCATTTTCATATCATTTCTGAGGTTTTAAACATAGAGCAAGTGGATCAAATGAAAGCAATGGAGAAGAATCTAATTGGGATGGCTAGAGAGAATGCGAGGTTTCATGGTTATGGAAGGCCTTGTGTTTCTTTCGGTATTCGACCACCAGGAGATGGAATAGTTCCGTATGGAAGCAGCAACAACGTTGCTGCTGGTGCTATTTGGGGAGCAGCATATGATCCCTCGCTTGATGCAGCTGGCTGTAGCTCTGAAGTCGGTGCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

33.24

Weight (kDa)

9.03

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 308
AccB7I CCANNNNNTGG 2 cut(s) 83, 773
AccBSI CCGCTC 1 cut(s) 486
AciI CCGC 1 cut(s) 486
AclI AACGTT 1 cut(s) 804
AclWI GGATC 2 cut(s) 672, 833
AcoI YGGCCR 2 cut(s) 103, 324
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 3 cut(s) 83, 101, 773
AgsI TTSAA 7 cut(s) 50, 332, 338, 397, 404, 506, 572
AjnI CCWGG 3 cut(s) 82, 201, 766
AluBI AGCT 6 cut(s) 300, 322, 478, 560, 857, 867
AluI AGCT 6 cut(s) 300, 322, 478, 560, 857, 867
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 1 cut(s) 4
AlwI GGATC 2 cut(s) 672, 833
AlwNI CAGNNNCTG 2 cut(s) 202, 436
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 5 cut(s) 92, 103, 324, 342, 737
ApeKI GCWGC 6 cut(s) 167, 436, 795, 809, 830, 854
ApoI RAATTY 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 200, 263
AvaI CYCGRG 1 cut(s) 54
AvaII GGWCC 1 cut(s) 74
BalI TGGCCA 2 cut(s) 105, 326
Bbv12I GWGCWC 1 cut(s) 883
BbvI GCAGC 6 cut(s) 179, 423, 796, 807, 842, 866
BccI CCATC 3 cut(s) 31, 694, 767
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 3 cut(s) 84, 203, 768
BcoDI GTCTC 1 cut(s) 4
BfaI CTAG 1 cut(s) 705
BfmI CTRYAG 2 cut(s) 156, 862
BglII AGATCT 1 cut(s) 532
BisI GCNGC 6 cut(s) 168, 437, 796, 810, 831, 855
BlsI GCNGC 6 cut(s) 169, 438, 797, 811, 832, 856
Bme1390I CCNGG 3 cut(s) 84, 203, 768
Bme18I GGWCC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 490
BmrFI CCNGG 3 cut(s) 84, 203, 768
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 3 cut(s) 54, 448, 841
BmuI ACTGGG 1 cut(s) 194
BpuEI CTTGAG 2 cut(s) 233, 436
BsaJI CCNNGG 2 cut(s) 83, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 101, 773
Bse1I ACTGG 1 cut(s) 189
Bse3DI GCAATG 2 cut(s) 77, 684
BseBI CCWGG 3 cut(s) 84, 203, 768
BseDI CCNNGG 2 cut(s) 83, 201
BseGI GGATG 2 cut(s) 66, 705
BseLI CCNNNNNNNGG 3 cut(s) 83, 101, 773
BseMI GCAATG 2 cut(s) 77, 684
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 189
BseXI GCAGC 6 cut(s) 179, 423, 796, 807, 842, 866
BshFI GGCC 5 cut(s) 94, 105, 326, 344, 739
BsiHKAI GWGCWC 1 cut(s) 883
BsiHKCI CYCGRG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 27
BslI CCNNNNNNNGG 3 cut(s) 83, 101, 773
BsmAI GTCTC 1 cut(s) 4
BsmBI CGTCTC 1 cut(s) 4
BsmFI GGGAC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 718
BsnI GGCC 5 cut(s) 94, 105, 326, 344, 739
BsoBI CYCGRG 1 cut(s) 54
Bsp1286I GDGCHC 1 cut(s) 883
Bsp143I GATC 5 cut(s) 110, 160, 532, 664, 838
BspACI CCGC 1 cut(s) 486
BspANI GGCC 5 cut(s) 94, 105, 326, 344, 739
BspCNI CTCAG 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 490
BspMAI CTGCAG 1 cut(s) 160
BspPI GGATC 2 cut(s) 672, 833
BsrBI CCGCTC 1 cut(s) 486
BsrDI GCAATG 2 cut(s) 77, 684
BsrI ACTGG 1 cut(s) 189
BssECI CCNNGG 2 cut(s) 83, 201
BssMI GATC 5 cut(s) 110, 160, 532, 664, 838
Bst2UI CCWGG 3 cut(s) 84, 203, 768
BstC8I GCNNGC 2 cut(s) 324, 859
BstDEI CTNAG 1 cut(s) 639
BstF5I GGATG 2 cut(s) 66, 705
BstKTI GATC 5 cut(s) 113, 163, 535, 667, 841
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 5 cut(s) 110, 160, 532, 664, 838
BstMWI GCNNNNNNNGC 2 cut(s) 164, 815
BstNI CCWGG 3 cut(s) 84, 203, 768
BstSCI CCNGG 3 cut(s) 82, 201, 766
BstSFI CTRYAG 2 cut(s) 156, 862
BstV1I GCAGC 6 cut(s) 179, 423, 796, 807, 842, 866
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuRI GGCC 5 cut(s) 94, 105, 326, 344, 739
BtsCI GGATG 2 cut(s) 66, 705
Cac8I GCNNGC 2 cut(s) 324, 859
CaiI CAGNNNCTG 2 cut(s) 202, 436
Cfr13I GGNCC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 20
CviAII CATG 3 cut(s) 71, 286, 725
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 1 cut(s) 639
DpnI GATC 5 cut(s) 112, 162, 534, 666, 840
DpnII GATC 5 cut(s) 110, 160, 532, 664, 838
DraI TTTAAA 1 cut(s) 648
EaeI YGGCCR 2 cut(s) 103, 324
Eco147I AGGCCT 1 cut(s) 739
Eco47I GGWCC 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 54
EcoRII CCWGG 3 cut(s) 82, 201, 766
Esp3I CGTCTC 1 cut(s) 4
FaeI CATG 3 cut(s) 74, 289, 728
FaqI GGGAC 1 cut(s) 27
FatI CATG 3 cut(s) 70, 285, 724
FauNDI CATATG 1 cut(s) 835
Fnu4HI GCNGC 6 cut(s) 168, 437, 796, 810, 831, 855
FokI GGATG 2 cut(s) 73, 712
Fsp4HI GCNGC 6 cut(s) 168, 437, 796, 810, 831, 855
FspBI CTAG 1 cut(s) 705
GluI GCNGC 6 cut(s) 168, 437, 796, 810, 831, 855
HaeIII GGCC 5 cut(s) 94, 105, 326, 344, 739
Hin1II CATG 3 cut(s) 74, 289, 728
HinfI GANTC 3 cut(s) 119, 545, 688
HphI GGTGA 2 cut(s) 200, 263
Hpy188I TCNGA 5 cut(s) 198, 381, 532, 640, 871
Hpy188III TCNNGA 4 cut(s) 54, 134, 212, 588
HpyAV CCTTC 4 cut(s) 259, 527, 531, 729
HpyCH4IV ACGT 2 cut(s) 40, 804
HpyCH4V TGCA 7 cut(s) 67, 158, 224, 274, 358, 439, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 815
HpyF3I CTNAG 1 cut(s) 639
HpySE526I ACGT 2 cut(s) 40, 804
Hsp92II CATG 3 cut(s) 74, 289, 728
Kzo9I GATC 5 cut(s) 110, 160, 532, 664, 838
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 6 cut(s) 179, 423, 796, 807, 842, 866
LweI GCATC 3 cut(s) 54, 448, 841
MaeI CTAG 1 cut(s) 705
MaeII ACGT 2 cut(s) 40, 804
MaeIII GTNAC 4 cut(s) 87, 120, 188, 598
MalI GATC 5 cut(s) 112, 162, 534, 666, 840
MbiI CCGCTC 1 cut(s) 486
MboI GATC 5 cut(s) 110, 160, 532, 664, 838
MboII GAAGA 3 cut(s) 229, 274, 697
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 1 cut(s) 883
MlsI TGGCCA 2 cut(s) 105, 326
MluCI AATT 7 cut(s) 269, 333, 353, 452, 554, 603, 693
MluNI TGGCCA 2 cut(s) 105, 326
MlyI GAGTC 2 cut(s) 128, 539
MnlI CCTC 9 cut(s) 50, 247, 283, 343, 411, 426, 634, 711, 853
Mox20I TGGCCA 2 cut(s) 105, 326
MroXI GAANNNNTTC 1 cut(s) 567
MscI TGGCCA 2 cut(s) 105, 326
MseI TTAA 3 cut(s) 540, 593, 647
MslI CAYNNNNRTG 2 cut(s) 505, 729
Msp20I TGGCCA 2 cut(s) 105, 326
MspA1I CMGCKG 1 cut(s) 857
MspR9I CCNGG 3 cut(s) 84, 203, 768
Mva1269I GAATGC 1 cut(s) 718
MvaI CCWGG 3 cut(s) 84, 203, 768
MwoI GCNNNNNNNGC 2 cut(s) 164, 815
NdeI CATATG 1 cut(s) 835
NdeII GATC 5 cut(s) 110, 160, 532, 664, 838
NlaIII CATG 3 cut(s) 74, 289, 728
NlaIV GGNNCC 1 cut(s) 490
NmuCI GTSAC 3 cut(s) 120, 188, 598
PaeR7I CTCGAG 1 cut(s) 54
PceI AGGCCT 1 cut(s) 739
PctI GAATGC 1 cut(s) 718
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 1 cut(s) 688
PflMI CCANNNNNTGG 2 cut(s) 83, 773
PkrI GCNGC 6 cut(s) 169, 438, 797, 811, 832, 856
PleI GAGTC 2 cut(s) 127, 539
PpsI GAGTC 2 cut(s) 127, 539
PsiI TTATAA 1 cut(s) 308
Psp1406I AACGTT 1 cut(s) 804
Psp6I CCWGG 3 cut(s) 82, 201, 766
PspGI CCWGG 3 cut(s) 82, 201, 766
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 2 cut(s) 202, 436
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 2 cut(s) 505, 729
SaqAI TTAA 3 cut(s) 540, 593, 647
SatI GCNGC 6 cut(s) 168, 437, 796, 810, 831, 855
Sau3AI GATC 5 cut(s) 110, 160, 532, 664, 838
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 2 cut(s) 128, 539
ScrFI CCNGG 3 cut(s) 84, 203, 768
SduI GDGCHC 1 cut(s) 883
SfaNI GCATC 3 cut(s) 54, 448, 841
SfcI CTRYAG 2 cut(s) 156, 862
Sfr274I CTCGAG 1 cut(s) 54
SinI GGWCC 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 54
SmiMI CAYNNNNRTG 2 cut(s) 505, 729
SmlI CTYRAG 3 cut(s) 54, 212, 415
SmoI CTYRAG 3 cut(s) 54, 212, 415
Sse9I AATT 7 cut(s) 269, 333, 353, 452, 554, 603, 693
SseBI AGGCCT 1 cut(s) 739
SsiI CCGC 1 cut(s) 486
SspMI CTAG 1 cut(s) 705
StuI AGGCCT 1 cut(s) 739
StyD4I CCNGG 3 cut(s) 82, 201, 766
TaiI ACGT 2 cut(s) 43, 807
TaqI TCGA 4 cut(s) 55, 367, 589, 760
TasI AATT 7 cut(s) 269, 333, 353, 452, 554, 603, 693
TfiI GAWTC 1 cut(s) 688
Tru1I TTAA 3 cut(s) 540, 593, 647
Tru9I TTAA 3 cut(s) 540, 593, 647
TseFI GTSAC 3 cut(s) 120, 188, 598
TseI GCWGC 6 cut(s) 167, 436, 795, 809, 830, 854
Tsp45I GTSAC 3 cut(s) 120, 188, 598
TspDTI ATGAA 5 cut(s) 489, 611, 617, 686, 713
TspGWI ACGGA 1 cut(s) 774
Van91I CCANNNNNTGG 2 cut(s) 83, 773
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 1 cut(s) 333
XhoI CTCGAG 1 cut(s) 54
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.