Rorug04G0052800

FLX-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002508
Physical Location & Seq
Forward (+)
8109694 .. 8118378
8685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0052800.1

Sequence Viewer

Length: 2709 bp
ATGGGAATCTTTGAGCCCTTCAGAGCAATCGGCTACACAACCACCTCCGTCCCCTTCTCCGTTCAGCGCCTCGGCACTGAAGCCTTCGTCACCGTCAGCGTCGGCAAAGCCTTCCAAATTTACAATTGCGCAAAGCTCACTCTGGTCCTCGTCGGTCCGCAATTGCCGAAGAAGATTCGAGCTCTTGCTTCGTATCGGGACTACACATTTGCTGCGTATGGGACTGATATAGCTGTTTTCAAGCGATCTCATCAGGTTGCTACGTGGAGCGCCCATAATGCGAAGGTCAATTTGTTGCTATTGTTTGGAGAGCATATCCTAAGTGTTGATGTGGAAGGTAATCTGTATATATGGACATTTAAAGGAATTGATGAGAATCTTGATCCGGTTGGACATATTAAGTTGGACGAAAATTTTACTCCTAGCTGTATAATGCATCCGGATACCTACTTAAATAAGGTTATCATTGGGAGTCAAGAAGGTTCTCTGCAGCTTTGGAATATCAGCACAAAGAAAAAACTGTATGAGTTCAAGGGATGGAAATCTTCTATATGCAGCTGTGTTTCGTCTCCTGCGCTAGATGTTGTTGCGGTTGGTTGTGCTGATGGAAAAATTCATGTTCACAACATACGATTTGATGAAGAGTTAGTGACGTTTTCCCATTCAACACGAGGTGCTGTGACTGCCTTATCTTTCAGCACAGATGGGCAGCCTCTTCTAGCATCTGGAGGTTCTTCTGGTGTTATAAGCATATGGAATCTCGAAAAAAGAAGGCTTCAATCAGTTATAAAAGAGGCACACGATGGTTCTATACTTTCACTGCACTTTTTTGTGAATGAACCAGTGTTAATGAGTTCATCATCAGACAATTCTATTAAAATGTGGATTTTTGACACAAGCGATGGAGATGCTCGTCTTTTACGGTTTAGAAGTGGTCATAGTGCTCCTCCACAATGCATAAGGTTTTATGCAAATGGGAGACATATTCTATCGGCTGGTCAGGATCGTGCCTTTCGCCTTTTCTCTATAATCCAGGATCAACAAAGTAGAGAGCTTTCTCAACGTCATATAACAAAACGAGCAAAGAAACTCAGGGTGAAGGAGGAAGAGATAAAACTGAAACCTGTCATTGCTTTTGATTGTGCTGAAATAAGAGAGCGAGATTGGTGCAATGTTGTTACATGTCATATGGATACTGCACAGGCATATGTCTGGAGACTTCAAAACTTTGTTCTTGGGGAGCATATTCTGAAACCAAGACCAGAAAACCCAACACCTGTGAAGGCCTGTGCCATCAGTGCATGTGGAAATTTTGCCATACTAGGGACCGCTGGTGGTTGGATTGAGCGGTTCAACCTGCAGTCAGGAAGCAGCCGAGGTAATTATGTGGATCCATCAGAAAGAAGCATCTGTGCCCATGACGGGGAAGTTGTTGGAATTGCTTGTGATTCAACTAATACTCACATGATAAGTGCTGGATATCACGGAGACATAAAGGTCTGGGATTTCAAGGGACGTGGAGTGCCATCTAGATGGGAAGTCGGGAGCTCTGTTGTCAGGATTGTATTCAATCGCTTGAATGGTCTTTTGGCTGTTGCGGCTGATGACTTAATTATCCGCTTGTTTGATGTTGTGGCATCAAGAATGGTCCGTAAATTTGAAGGCCATACTGATCGTGTTACAGACATGTGTTTTAGTGAGGATGGAAAATGGCTTTTGTCTTCCAGTATGGATGGAAGTCTTCGAGTTTGGGATGTTATCTTAGCAAGACAAATAGATGCGTTGCATGTTGATGTGTCTGTGACTGCATTGTCTCTATCACCGAATATGGATATTTTAGCGACCTCCCATGTTGATCAAAATGGGGTCTATCTTTGGGTGAATCAATCAATGTTTTCGGGAGCTTCAAAGGTAGATTCCTATGCAAGTGGAAAGCGAGTTGTGAGTGTTAAGTTGCCATCTGTCTCTTCGACAAAAGGTTCTGAAGCTGAGGATTCTGATGAGCCTGTTGGAAACTCTGAACAACTGAACGATGCTTCTGCTCTTCCAAGTCCTGTACAGCAAATTCCAGATCTTGTTACTCTCTCATCATATCCTAAAAGCCAGTGGCAAAGCTTAATCAACTTAGACATTATAAAGGAACGAAATAAACCGATTGAGCCCCCAAAGAAACCTGAAAAGGCTCCTTTCTTTTTGCCTTCAGTTCCATCTCTTTCAGGAGAAATAATGTTTAAGCCTACTGGGTCGACAAATGAGGAGGCAAAAGGTGGTGAAGAATCATCTAGAATAAAGGCTGCTCTAGCGCCATCAATATTCCTTCAAGCGCTTCAGTCATCTGCAGAAATGAAGAATTTTTCAGAATTTACTGATTTCATCAAAACTTTGTCTCCATCATCCCTGGATATGGAACTTCGAATTCTTCAGATTGTAGGTGATGAGGATGAAGAAGAGCCAGAAAAGTTGCTTCCAATTGAACTGCTTCTCGACTATTTTATACACGAGACTGCATGCAGAAACAATTTTGACTTTGTACAAGCTGTGATCAGGGTTTTTTTGAAGATACATGGCGAAACAATTCGACGCCAGTCAAGACTACAGGATAAGGCAAGCAAGCTCCTAGACGTTCAATGCAACACATGGCAAAGAGTAGATAAAATGTTTCAGGGTGCCAGGTGCATGGTCACTTTTCTTAGCAATTCACAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

902

Amino Acids

100.34

Weight (kDa)

6.56

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 30 - 298 2.1e-100 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 103 - 260 2.8e-15 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 123 - 353 2.3e-16 G protein beta WD-40 repeat protein
Beta-prop_RIG_2nd PF23775 134 - 287 2.6e-07 RIG second beta-propeller
WD40_Prp19 PF24814 143 - 295 4e-17 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 146 - 299 3e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 148 - 259 4.2e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 152 - 258 1.6e-06 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 153 - 260 3.9e-12 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 154 - 257 3.9e-10 THOC3 beta-propeller domain
Beta-prop_HPS5 PF23756 154 - 286 1.9e-07 HPS5 beta-propeller
Beta-prop_WDR3_1st PF25173 155 - 343 2.6e-25 WDR3 first beta-propeller domain
WDR90_beta-prop_4th PF23342 156 - 273 2e-07 WDR90, 4th beta-propeller
Beta-prop_WDR5 PF25175 157 - 356 2.5e-21 WDR5 beta-propeller domain
Beta-prop_EML PF23409 157 - 298 7.2e-15 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 159 - 262 6.1e-07 WDHD1 first WD40 domain
Beta-prop_Aladin PF25460 175 - 343 2.9e-07 Aladin seven-bladed propeller
WDR55 PF24796 183 - 295 3.8e-10 WDR55
Beta-prop_VPS8 PF23410 194 - 290 6.5e-07 Vps8 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 223 - 360 2e-10 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 225 - 345 1.3e-10 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 226 - 299 5.7e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 227 - 343 3.6e-09 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 239 - 343 4.3e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 240 - 358 2.8e-08 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 319 - 636 4.6e-103 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 427 - 626 2.2e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 433 - 615 3.8e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 468 - 616 1.8e-14 Prp19 WD40 domain
WDR55 PF24796 472 - 586 2.6e-07 WDR55
WD40_MABP1-WDR62_2nd PF24782 481 - 585 7.7e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 482 - 618 2.1e-08 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 516 - 631 8.3e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 516 - 621 1.8e-16 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 517 - 597 5.3e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 518 - 592 2.7e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 528 - 627 6e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR75_1st PF23869 533 - 609 9.4e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 549 - 585 4.5e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 550 - 621 4.7e-07 CAF1B/HIR1 beta-propeller domain
Utp21 PF04192 688 - 898 1.8e-53 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 746, 788, 2135
AasI GACNNNNNNGTC 2 cut(s) 1496, 1810
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 1365
AccB1I GGYRCC 1 cut(s) 2666
AccBSI CCGCTC 1 cut(s) 1348
AccI GTMKAC 1 cut(s) 2246
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 6 cut(s) 158, 590, 1329, 1348, 1598, 1618
AclWI GGATC 5 cut(s) 377, 1011, 1044, 1385, 1398
AcsI RAATTY 9 cut(s) 117, 412, 612, 1309, 1655, 2064, 2350, 2360, 2415
AcuI CTGAAG 6 cut(s) 4, 99, 2004, 2184, 2312, 2405
AcyI GRCGYC 1 cut(s) 2581
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 2 cut(s) 2058, 2532
AfeI AGCGCT 1 cut(s) 2325
AfiI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
AflIII ACRYGT 2 cut(s) 1181, 1686
AjiI CACGTC 1 cut(s) 1517
AjnI CCWGG 3 cut(s) 1032, 2397, 2669
AjuI GAANNNNNNNTTGG 2 cut(s) 1571, 1603
Alw21I GWGCWC 3 cut(s) 184, 946, 1550
Alw26I GTCTC 8 cut(s) 573, 973, 1210, 1482, 1818, 1969, 2391, 2495
AlwI GGATC 5 cut(s) 377, 1011, 1044, 1385, 1398
Aor13HI TCCGGA 1 cut(s) 439
Aor51HI AGCGCT 1 cut(s) 2325
AoxI GGCC 2 cut(s) 1284, 1663
ApeKI GCWGC 6 cut(s) 212, 490, 555, 709, 1371, 2294
ApoI RAATTY 9 cut(s) 117, 412, 612, 1309, 1655, 2064, 2350, 2360, 2415
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 2 cut(s) 2478, 2574
AspLEI GCGC 6 cut(s) 69, 131, 272, 577, 2305, 2326
AspS9I GGNCC 4 cut(s) 145, 155, 1326, 1648
AsuHPI GGTGA 6 cut(s) 82, 1108, 1812, 1891, 2282, 2444
AsuII TTCGAA 1 cut(s) 2413
AvaII GGWCC 4 cut(s) 145, 155, 1326, 1648
BaeGI GKGCMC 1 cut(s) 1417
BamHI GGATCC 1 cut(s) 1390
BanI GGYRCC 1 cut(s) 2666
BanII GRGCYC 4 cut(s) 18, 184, 1550, 2163
BauI CACGAG 2 cut(s) 669, 2498
BbsI GAAGAC 2 cut(s) 1713, 1733
Bbv12I GWGCWC 3 cut(s) 184, 946, 1550
BbvCI CCTCAGC 1 cut(s) 1989
BbvI GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
BcgI CGANNNNNNTGC 6 cut(s) 890, 924, 1149, 1183, 2021, 2055
BciT130I CCWGG 3 cut(s) 1034, 2399, 2671
BciVI GTATCC 2 cut(s) 436, 1186
BclI TGATCA 2 cut(s) 1855, 2541
BcoDI GTCTC 8 cut(s) 573, 973, 1210, 1482, 1818, 1969, 2391, 2495
BfaI CTAG 8 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2618
BfmI CTRYAG 4 cut(s) 488, 1358, 2337, 2594
BfoI RGCGCY 4 cut(s) 70, 273, 2306, 2327
BfuAI ACCTGC 1 cut(s) 1365
BfuI GTATCC 2 cut(s) 436, 1186
BglII AGATCT 1 cut(s) 2071
BisI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
BlsI GCNGC 7 cut(s) 214, 492, 557, 711, 1373, 1600, 2296
Bme1390I CCNGG 3 cut(s) 1034, 2399, 2671
Bme18I GGWCC 4 cut(s) 145, 155, 1326, 1648
BmgBI CACGTC 1 cut(s) 1517
BmgT120I GGNCC 4 cut(s) 145, 155, 1326, 1648
BmiI GGNNCC 4 cut(s) 1327, 1392, 2184, 2668
BmrFI CCNGG 3 cut(s) 1034, 2399, 2671
BmrI ACTGGG 1 cut(s) 2250
BmsI GCATC 7 cut(s) 445, 731, 898, 1416, 1646, 1768, 2023
BmuI ACTGGG 1 cut(s) 2250
BoxI GACNNNNGTC 1 cut(s) 2584
BpiI GAAGAC 2 cut(s) 1713, 1733
BpmI CTGGAG 2 cut(s) 747, 1234
Bpu10I CCTNAGC 1 cut(s) 1989
Bpu14I TTCGAA 1 cut(s) 2413
BsaAI YACGTR 1 cut(s) 264
BsaBI GATNNNNATC 2 cut(s) 375, 541
BsaHI GRCGYC 1 cut(s) 2581
BsaJI CCNNGG 3 cut(s) 70, 1375, 2397
BsaWI WCCGGW 2 cut(s) 385, 439
BsaXI ACNNNNNCTCC 2 cut(s) 2371, 2401
Bsc4I CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
Bse1I ACTGG 5 cut(s) 842, 1725, 2104, 2245, 2584
Bse3DI GCAATG 2 cut(s) 1128, 1177
Bse8I GATNNNNATC 2 cut(s) 375, 541
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 3 cut(s) 1034, 2399, 2671
BseDI CCNNGG 3 cut(s) 70, 1375, 2397
BseGI GGATG 7 cut(s) 436, 542, 1708, 1738, 1759, 2393, 2446
BseJI GATNNNNATC 2 cut(s) 375, 541
BseLI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
BseMI GCAATG 2 cut(s) 1128, 1177
BseMII CTCAG 2 cut(s) 1105, 1980
BseNI ACTGG 5 cut(s) 842, 1725, 2104, 2245, 2584
BseRI GAGGAG 2 cut(s) 936, 2270
BseSI GKGCMC 1 cut(s) 1417
BseXI GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
BsgI GTGCAG 2 cut(s) 806, 1182
BshFI GGCC 2 cut(s) 1286, 1665
BshNI GGYRCC 1 cut(s) 2666
BsiHKAI GWGCWC 3 cut(s) 184, 946, 1550
BsiSI CCGG 2 cut(s) 386, 440
BslFI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
BslI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
BsmAI GTCTC 8 cut(s) 573, 973, 1210, 1482, 1818, 1969, 2391, 2495
BsmBI CGTCTC 1 cut(s) 573
BsmFI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
BsnI GGCC 2 cut(s) 1286, 1665
Bsp119I TTCGAA 1 cut(s) 2413
Bsp1286I GDGCHC 6 cut(s) 18, 184, 946, 1417, 1550, 2163
Bsp13I TCCGGA 1 cut(s) 439
Bsp1407I TGTACA 2 cut(s) 2056, 2530
Bsp143I GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
BspACI CCGC 6 cut(s) 158, 590, 1329, 1348, 1598, 1618
BspANI GGCC 2 cut(s) 1286, 1665
BspCNI CTCAG 2 cut(s) 1104, 1981
BspEI TCCGGA 1 cut(s) 439
BspLI GGNNCC 4 cut(s) 1327, 1392, 2184, 2668
BspMAI CTGCAG 3 cut(s) 492, 1362, 2341
BspMI ACCTGC 1 cut(s) 1365
BspPI GGATC 5 cut(s) 377, 1011, 1044, 1385, 1398
BspQI GCTCTTC 2 cut(s) 2049, 2442
BspT104I TTCGAA 1 cut(s) 2413
BspT107I GGYRCC 1 cut(s) 2666
BsrBI CCGCTC 1 cut(s) 1348
BsrDI GCAATG 2 cut(s) 1128, 1177
BsrGI TGTACA 2 cut(s) 2056, 2530
BsrI ACTGG 5 cut(s) 842, 1725, 2104, 2245, 2584
BssECI CCNNGG 3 cut(s) 70, 1375, 2397
BssMI GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
BssNI GRCGYC 1 cut(s) 2581
BssSI CACGAG 2 cut(s) 669, 2498
Bst2BI CACGAG 2 cut(s) 669, 2498
Bst2UI CCWGG 3 cut(s) 1034, 2399, 2671
Bst4CI ACNGT 4 cut(s) 94, 522, 924, 2703
Bst6I CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2049, 2442
BstACI GRCGYC 1 cut(s) 2581
BstAUI TGTACA 2 cut(s) 2056, 2530
BstBAI YACGTR 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 2413
BstC8I GCNNGC 3 cut(s) 2509, 2608, 2612
BstDEI CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2125, 2690
BstF5I GGATG 7 cut(s) 436, 542, 1708, 1738, 1759, 2393, 2446
BstH2I RGCGCY 4 cut(s) 70, 273, 2306, 2327
BstHHI GCGC 6 cut(s) 69, 131, 272, 577, 2305, 2326
BstKTI GATC 9 cut(s) 248, 385, 1006, 1039, 1393, 1675, 1858, 2074, 2544
BstMAI GTCTC 8 cut(s) 573, 973, 1210, 1482, 1818, 1969, 2391, 2495
BstMBI GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
BstMWI GCNNNNNNNGC 5 cut(s) 278, 683, 1298, 1598, 2300
BstNI CCWGG 3 cut(s) 1034, 2399, 2671
BstNSI RCATGY 5 cut(s) 1185, 1305, 1690, 1790, 2511
BstPAI GACNNNNGTC 1 cut(s) 2584
BstSCI CCNGG 3 cut(s) 1032, 2397, 2669
BstSFI CTRYAG 4 cut(s) 488, 1358, 2337, 2594
BstSLI GKGCMC 1 cut(s) 1417
BstV1I GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
BstV2I GAAGAC 2 cut(s) 1713, 1733
BstX2I RGATCY 2 cut(s) 1390, 2071
BstXI CCANNNNNNTGG 2 cut(s) 1533, 2677
BstYI RGATCY 2 cut(s) 1390, 2071
BsuI GTATCC 2 cut(s) 436, 1186
BsuRI GGCC 2 cut(s) 1286, 1665
BtgZI GCGATG 1 cut(s) 915
BtrI CACGTC 1 cut(s) 1517
BtsCI GGATG 7 cut(s) 436, 542, 1708, 1738, 1759, 2393, 2446
BtsI GCAGTG 1 cut(s) 818
BtsIMutI CAGTG 5 cut(s) 75, 818, 849, 1303, 2111
BveI ACCTGC 1 cut(s) 1365
Cac8I GCNNGC 3 cut(s) 2509, 2608, 2612
CfoI GCGC 6 cut(s) 69, 131, 272, 577, 2305, 2326
Cfr13I GGNCC 4 cut(s) 145, 155, 1326, 1648
CpoI CGGWCCG 1 cut(s) 155
CseI GACGC 2 cut(s) 88, 2589
Csp6I GTAC 2 cut(s) 2057, 2531
CspCI CAANNNNNGTGG 2 cut(s) 1498, 1533
CspI CGGWCCG 1 cut(s) 155
CviQI GTAC 2 cut(s) 2057, 2531
DdeI CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2125, 2690
DpnI GATC 9 cut(s) 247, 384, 1005, 1038, 1392, 1674, 1857, 2073, 2543
DpnII GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 674
DrdI GACNNNNNNGTC 2 cut(s) 1496, 1810
DseDI GACNNNNNNGTC 2 cut(s) 1496, 1810
Eam1104I CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2049, 2442
EarI CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2049, 2442
Ecl136II GAGCTC 2 cut(s) 182, 1548
Eco147I AGGCCT 1 cut(s) 1286
Eco24I GRGCYC 4 cut(s) 18, 184, 1550, 2163
Eco32I GATATC 1 cut(s) 1481
Eco47I GGWCC 4 cut(s) 145, 155, 1326, 1648
Eco47III AGCGCT 1 cut(s) 2325
Eco53kI GAGCTC 2 cut(s) 182, 1548
Eco57I CTGAAG 6 cut(s) 4, 99, 2004, 2184, 2312, 2405
EcoICRI GAGCTC 2 cut(s) 182, 1548
EcoRI GAATTC 1 cut(s) 2415
EcoRII CCWGG 3 cut(s) 1032, 2397, 2669
EcoRV GATATC 1 cut(s) 1481
EcoT22I ATGCAT 2 cut(s) 438, 959
EcoT38I GRGCYC 4 cut(s) 18, 184, 1550, 2163
Esp3I CGTCTC 1 cut(s) 573
FaqI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
FauNDI CATATG 3 cut(s) 752, 1188, 1207
FbaI TGATCA 2 cut(s) 1855, 2541
FblI GTMKAC 1 cut(s) 2246
Fnu4HI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
FokI GGATG 7 cut(s) 423, 549, 1715, 1745, 1766, 2380, 2453
FriOI GRGCYC 4 cut(s) 18, 184, 1550, 2163
Fsp4HI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
FspBI CTAG 8 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2618
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 6 cut(s) 68, 130, 271, 576, 2304, 2325
GluI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
GsuI CTGGAG 2 cut(s) 747, 1234
HaeII RGCGCY 4 cut(s) 70, 273, 2306, 2327
HaeIII GGCC 2 cut(s) 1286, 1665
HapII CCGG 2 cut(s) 386, 440
HgaI GACGC 2 cut(s) 88, 2589
HhaI GCGC 6 cut(s) 69, 131, 272, 577, 2305, 2326
Hin1I GRCGYC 1 cut(s) 2581
Hin6I GCGC 6 cut(s) 67, 129, 270, 575, 2303, 2324
HinP1I GCGC 6 cut(s) 67, 129, 270, 575, 2303, 2324
HincII GTYRAC 1 cut(s) 2247
HindII GTYRAC 1 cut(s) 2247
HindIII AAGCTT 1 cut(s) 2113
HpaII CCGG 2 cut(s) 386, 440
HphI GGTGA 6 cut(s) 82, 1108, 1812, 1891, 2282, 2444
Hpy166II GTNNAC 2 cut(s) 622, 2247
Hpy188I TCNGA 9 cut(s) 23, 865, 1251, 1399, 1984, 1999, 2020, 2359, 2424
Hpy8I GTNNAC 2 cut(s) 622, 2247
Hpy99I CGWCG 3 cut(s) 104, 155, 2583
HpyCH4III ACNGT 4 cut(s) 94, 522, 924, 2703
HpyCH4IV ACGT 5 cut(s) 263, 653, 1063, 1516, 2622
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 683, 1298, 1598, 2300
HpyF3I CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2125, 2690
HpySE526I ACGT 5 cut(s) 263, 653, 1063, 1516, 2622
Hsp92I GRCGYC 1 cut(s) 2581
HspAI GCGC 6 cut(s) 67, 129, 270, 575, 2303, 2324
Kpn2I TCCGGA 1 cut(s) 439
Ksp22I TGATCA 2 cut(s) 1855, 2541
Kzo9I GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
LguI GCTCTTC 2 cut(s) 2049, 2442
LmnI GCTCC 7 cut(s) 267, 949, 1240, 1545, 1901, 2188, 2619
Lsp1109I GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
LweI GCATC 7 cut(s) 445, 731, 898, 1416, 1646, 1768, 2023
MaeI CTAG 8 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2618
MaeII ACGT 5 cut(s) 263, 653, 1063, 1516, 2622
MaeIII GTNAC 8 cut(s) 88, 649, 679, 1177, 1678, 1801, 2077, 2680
MalI GATC 9 cut(s) 247, 384, 1005, 1038, 1392, 1674, 1857, 2073, 2543
MbiI CCGCTC 1 cut(s) 1348
MboI GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
MfeI CAATTG 3 cut(s) 124, 161, 2469
MflI RGATCY 2 cut(s) 1390, 2071
MhlI GDGCHC 6 cut(s) 18, 184, 946, 1417, 1550, 2163
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 5 cut(s) 370, 384, 1319, 1414, 1990
Mph1103I ATGCAT 2 cut(s) 438, 959
MroI TCCGGA 1 cut(s) 439
MroXI GAANNNNTTC 2 cut(s) 2478, 2574
MseI TTAA 9 cut(s) 360, 399, 452, 848, 876, 1610, 1950, 2117, 2232
MslI CAYNNNNRTG 2 cut(s) 1531, 1791
MspA1I CMGCKG 2 cut(s) 558, 1331
MspI CCGG 2 cut(s) 386, 440
MspR9I CCNGG 3 cut(s) 1034, 2399, 2671
MunI CAATTG 3 cut(s) 124, 161, 2469
MvaI CCWGG 3 cut(s) 1034, 2399, 2671
MwoI GCNNNNNNNGC 5 cut(s) 278, 683, 1298, 1598, 2300
NdeI CATATG 3 cut(s) 752, 1188, 1207
NdeII GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
NlaIV GGNNCC 4 cut(s) 1327, 1392, 2184, 2668
NmeAIII GCCGAG 2 cut(s) 51, 1400
NmuCI GTSAC 5 cut(s) 88, 649, 679, 1801, 2680
NsbI TGCGCA 1 cut(s) 130
NsiI ATGCAT 2 cut(s) 438, 959
NspI RCATGY 5 cut(s) 1185, 1305, 1690, 1790, 2511
NspV TTCGAA 1 cut(s) 2413
PaeI GCATGC 1 cut(s) 2511
PceI AGGCCT 1 cut(s) 1286
PciI ACATGT 2 cut(s) 1181, 1686
PciSI GCTCTTC 2 cut(s) 2049, 2442
PdmI GAANNNNTTC 2 cut(s) 2478, 2574
PfeI GAWTC 9 cut(s) 6, 175, 376, 757, 1448, 1882, 1916, 1994, 2276
PfoI TCCNGGA 1 cut(s) 1032
PkrI GCNGC 7 cut(s) 214, 492, 557, 711, 1373, 1600, 2296
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
Ppu21I YACGTR 1 cut(s) 264
PscI ACATGT 2 cut(s) 1181, 1686
PshAI GACNNNNGTC 1 cut(s) 2584
PsiI TTATAA 3 cut(s) 746, 788, 2135
Psp124BI GAGCTC 2 cut(s) 184, 1550
Psp6I CCWGG 3 cut(s) 1032, 2397, 2669
PspGI CCWGG 3 cut(s) 1032, 2397, 2669
PspN4I GGNNCC 4 cut(s) 1327, 1392, 2184, 2668
PspPI GGNCC 4 cut(s) 145, 155, 1326, 1648
PstI CTGCAG 3 cut(s) 492, 1362, 2341
PsuI RGATCY 2 cut(s) 1390, 2071
PvuII CAGCTG 1 cut(s) 558
RsaI GTAC 2 cut(s) 2058, 2532
RsaNI GTAC 2 cut(s) 2057, 2531
RseI CAYNNNNRTG 2 cut(s) 1531, 1791
Rsr2I CGGWCCG 1 cut(s) 155
RsrII CGGWCCG 1 cut(s) 155
SacI GAGCTC 2 cut(s) 184, 1550
SalI GTCGAC 1 cut(s) 2245
SapI GCTCTTC 2 cut(s) 2049, 2442
SaqAI TTAA 9 cut(s) 360, 399, 452, 848, 876, 1610, 1950, 2117, 2232
SatI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
Sau3AI GATC 9 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071, 2541
Sau96I GGNCC 4 cut(s) 145, 155, 1326, 1648
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 3 cut(s) 1034, 2399, 2671
SduI GDGCHC 6 cut(s) 18, 184, 946, 1417, 1550, 2163
SfaNI GCATC 7 cut(s) 445, 731, 898, 1416, 1646, 1768, 2023
SfcI CTRYAG 4 cut(s) 488, 1358, 2337, 2594
SfuI TTCGAA 1 cut(s) 2413
SinI GGWCC 4 cut(s) 145, 155, 1326, 1648
SmiMI CAYNNNNRTG 2 cut(s) 1531, 1791
SphI GCATGC 1 cut(s) 2511
SseBI AGGCCT 1 cut(s) 1286
SsiI CCGC 6 cut(s) 158, 590, 1329, 1348, 1598, 1618
SspI AATATT 1 cut(s) 2313
SspMI CTAG 8 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2618
SstI GAGCTC 2 cut(s) 184, 1550
StuI AGGCCT 1 cut(s) 1286
StyD4I CCNGG 3 cut(s) 1032, 2397, 2669
TaaI ACNGT 4 cut(s) 94, 522, 924, 2703
TaiI ACGT 5 cut(s) 266, 656, 1066, 1519, 2625
TaqI TCGA 8 cut(s) 178, 762, 1744, 1970, 2246, 2413, 2484, 2578
TaqII GACCGA 1 cut(s) 143
TatI WGTACW 2 cut(s) 2056, 2530
TauI GCSGC 1 cut(s) 1601
TfiI GAWTC 9 cut(s) 6, 175, 376, 757, 1448, 1882, 1916, 1994, 2276
Tru1I TTAA 9 cut(s) 360, 399, 452, 848, 876, 1610, 1950, 2117, 2232
Tru9I TTAA 9 cut(s) 360, 399, 452, 848, 876, 1610, 1950, 2117, 2232
TscAI CASTG 5 cut(s) 82, 825, 849, 1303, 2111
TseFI GTSAC 5 cut(s) 88, 649, 679, 1801, 2680
TseI GCWGC 6 cut(s) 212, 490, 555, 709, 1371, 2294
Tsp45I GTSAC 5 cut(s) 88, 649, 679, 1801, 2680
TspDTI ATGAA 7 cut(s) 605, 654, 846, 852, 2360, 2362, 2457
TspGWI ACGGA 4 cut(s) 37, 49, 1500, 1640
TspRI CASTG 5 cut(s) 82, 825, 849, 1303, 2111
VpaK11BI GGWCC 4 cut(s) 145, 155, 1326, 1648
XapI RAATTY 9 cut(s) 117, 412, 612, 1309, 1655, 2064, 2350, 2360, 2415
XbaI TCTAGA 2 cut(s) 1529, 2282
XceI RCATGY 5 cut(s) 1185, 1305, 1690, 1790, 2511
XmiI GTMKAC 1 cut(s) 2246
XmnI GAANNNNTTC 2 cut(s) 2478, 2574
XspI CTAG 8 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2618
Zsp2I ATGCAT 2 cut(s) 438, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.