RLG00000008879

FLX-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002508
Physical Location & Seq
Forward (+)
41707882 .. 41709579
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008879

Sequence Viewer

Length: 711 bp
ATGGCTGGGCGACGACATATTTTACCATCCTTTGACAGACGCCCTATACAGTCTCCAGGGATGATGCGGCACGGGCCATTGCCTGGGTTAGGTCGTGCTCCTGGCTACCGAGTTTTGGAGCCAGTACCCCGTGCTGAACTACTGGAAAACAAAATTGCATATCAAGCAGCAGAGATAAAACAACTTGCAGGAGACAATCATAGATTGGCAGATACTGAGGTGGATTTGAGGCGGGAGGTTGTAGCTGCAGAGGAGGAAGTTCAAACAGTCAAGGCACACATGAGAAGCATCCAGACTGAAAGCGACATCCAGATCCGAGCGTTGCTTGATAAGATTGTAAAAAAGGAACAGGAAATTGTAGCCGGTGAGGGTGTGAAGAAGGAATTGCAAAAGGCTCACATGGAAGCACAGAGCTTGGTGGAAGCACGGAAAGAGCTAAGAATTCAAATTCGACAGGCCACTGATGAGTTGCAGAAAGCTCGTGTTGAGGTTAAGAAACTGCCAGATCTGCATGGTGAACTTGATAGTTTGAGACAAGAACACCAGAGGCTGCGTGCTACTTTTGAATATGAAAAAGGTAGAAACATAGAACAAGTCGAGCAAATGAAAGTAATGGAGAAGAATCTAATGGGCTTGGCAGGAGAAGTAGAAAGGTTGCGTGCTGAGGTCTTGAATGCAGAGAAGAGAGCACAAGGTTACTATTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

27.02

Weight (kDa)

8.69

Isoelectric Point (pI)

54.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LAMB4 PF24999 136 - 187 2.4e-06 Laminin subunit beta-4-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 83
AciI CCGC 2 cut(s) 67, 232
AclWI GGATC 1 cut(s) 307
AcsI RAATTY 2 cut(s) 441, 447
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 3 cut(s) 83, 89, 115
AgsI TTSAA 4 cut(s) 263, 446, 566, 673
AjnI CCWGG 3 cut(s) 55, 82, 100
AluBI AGCT 4 cut(s) 245, 414, 436, 479
AluI AGCT 4 cut(s) 245, 414, 436, 479
Alw21I GWGCWC 2 cut(s) 100, 691
Alw26I GTCTC 3 cut(s) 57, 186, 526
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 2 cut(s) 215, 550
AoxI GGCC 2 cut(s) 74, 456
ApeKI GCWGC 3 cut(s) 167, 245, 550
ApoI RAATTY 2 cut(s) 441, 447
AspS9I GGNCC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 377, 527
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BauI CACGAG 1 cut(s) 480
Bbv12I GWGCWC 2 cut(s) 100, 691
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 3 cut(s) 179, 232, 537
BccI CCATC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 3 cut(s) 57, 84, 102
BcoDI GTCTC 3 cut(s) 57, 186, 526
BfmI CTRYAG 1 cut(s) 246
BglII AGATCT 1 cut(s) 505
BisI GCNGC 4 cut(s) 68, 168, 246, 551
BlsI GCNGC 4 cut(s) 69, 169, 247, 552
Bme1390I CCNGG 3 cut(s) 57, 84, 102
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 57, 84, 102
BmsI GCATC 2 cut(s) 54, 297
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 663
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 56, 83
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 89, 115
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 2 cut(s) 122, 147
Bse3DI GCAATG 1 cut(s) 77
BseBI CCWGG 3 cut(s) 57, 84, 102
BseDI CCNNGG 2 cut(s) 56, 83
BseGI GGATG 4 cut(s) 26, 66, 288, 306
BseLI CCNNNNNNNGG 3 cut(s) 83, 89, 115
BseMI GCAATG 1 cut(s) 77
BseMII CTCAG 2 cut(s) 207, 654
BseNI ACTGG 2 cut(s) 122, 147
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 3 cut(s) 179, 232, 537
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 2 cut(s) 76, 458
BsiHKAI GWGCWC 2 cut(s) 100, 691
BsiSI CCGG 1 cut(s) 363
BslI CCNNNNNNNGG 3 cut(s) 83, 89, 115
BsmAI GTCTC 3 cut(s) 57, 186, 526
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 2 cut(s) 76, 458
Bsp1286I GDGCHC 2 cut(s) 100, 691
Bsp143I GATC 2 cut(s) 312, 505
BspACI CCGC 2 cut(s) 67, 232
BspANI GGCC 2 cut(s) 76, 458
BspCNI CTCAG 2 cut(s) 208, 655
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 1 cut(s) 250
BspPI GGATC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 77
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 2 cut(s) 122, 147
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 2 cut(s) 56, 83
BssMI GATC 2 cut(s) 312, 505
BssNI GRCGYC 1 cut(s) 40
BssSI CACGAG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 480
Bst2UI CCWGG 3 cut(s) 57, 84, 102
Bst4CI ACNGT 2 cut(s) 51, 268
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 555, 660
BstDEI CTNAG 3 cut(s) 216, 437, 663
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 4 cut(s) 26, 66, 288, 306
BstKTI GATC 2 cut(s) 315, 508
BstMAI GTCTC 3 cut(s) 57, 186, 526
BstMBI GATC 2 cut(s) 312, 505
BstMWI GCNNNNNNNGC 3 cut(s) 73, 164, 508
BstNI CCWGG 3 cut(s) 57, 84, 102
BstSCI CCNGG 3 cut(s) 55, 82, 100
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 179, 232, 537
BstX2I RGATCY 2 cut(s) 312, 505
BstYI RGATCY 2 cut(s) 312, 505
BsuRI GGCC 2 cut(s) 76, 458
BtsCI GGATG 4 cut(s) 26, 66, 288, 306
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 2 cut(s) 555, 660
CaiI CAGNNNCTG 2 cut(s) 215, 550
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 74
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 125
CviAII CATG 3 cut(s) 280, 400, 512
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 3 cut(s) 216, 437, 663
DpnI GATC 2 cut(s) 314, 507
DpnII GATC 2 cut(s) 312, 505
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 441
EcoRII CCWGG 3 cut(s) 55, 82, 100
FaeI CATG 3 cut(s) 283, 403, 515
FatI CATG 3 cut(s) 279, 399, 511
FauI CCCGC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 68, 168, 246, 551
FokI GGATG 4 cut(s) 13, 73, 275, 293
Fsp4HI GCNGC 4 cut(s) 68, 168, 246, 551
GluI GCNGC 4 cut(s) 68, 168, 246, 551
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 2 cut(s) 76, 458
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 3 cut(s) 283, 403, 515
HinfI GANTC 1 cut(s) 622
HpaII CCGG 1 cut(s) 363
HphI GGTGA 2 cut(s) 377, 527
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 3 cut(s) 292, 310, 670
Hpy8I GTNNAC 1 cut(s) 518
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 2 cut(s) 51, 268
HpyCH4V TGCA 7 cut(s) 158, 188, 248, 388, 472, 511, 677
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 164, 508
HpyF3I CTNAG 3 cut(s) 216, 437, 663
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 3 cut(s) 283, 403, 515
Kzo9I GATC 2 cut(s) 312, 505
LmnI GCTCC 2 cut(s) 103, 118
Lsp1109I GCAGC 3 cut(s) 179, 232, 537
LweI GCATC 2 cut(s) 54, 297
MaeIII GTNAC 1 cut(s) 695
MalI GATC 2 cut(s) 314, 507
MboI GATC 2 cut(s) 312, 505
MboII GAAGA 3 cut(s) 388, 631, 694
MflI RGATCY 2 cut(s) 312, 505
MhlI GDGCHC 2 cut(s) 100, 691
MluCI AATT 5 cut(s) 153, 354, 383, 441, 447
MnlI CCTC 9 cut(s) 211, 222, 229, 244, 247, 361, 481, 540, 658
MseI TTAA 1 cut(s) 492
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 3 cut(s) 57, 84, 102
Mva1269I GAATGC 1 cut(s) 679
MvaI CCWGG 3 cut(s) 57, 84, 102
MwoI GCNNNNNNNGC 3 cut(s) 73, 164, 508
NdeII GATC 2 cut(s) 312, 505
NlaIII CATG 3 cut(s) 283, 403, 515
NlaIV GGNNCC 1 cut(s) 120
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 1 cut(s) 622
PflMI CCANNNNNTGG 1 cut(s) 83
PkrI GCNGC 4 cut(s) 69, 169, 247, 552
Psp6I CCWGG 3 cut(s) 55, 82, 100
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 55, 82, 100
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 1 cut(s) 250
PstNI CAGNNNCTG 2 cut(s) 215, 550
PsuI RGATCY 2 cut(s) 312, 505
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 4 cut(s) 68, 168, 246, 551
Sau3AI GATC 2 cut(s) 312, 505
Sau96I GGNCC 1 cut(s) 74
ScrFI CCNGG 3 cut(s) 57, 84, 102
SduI GDGCHC 2 cut(s) 100, 691
SfaNI GCATC 2 cut(s) 54, 297
SfcI CTRYAG 1 cut(s) 246
Sse9I AATT 5 cut(s) 153, 354, 383, 441, 447
SsiI CCGC 2 cut(s) 67, 232
StyD4I CCNGG 3 cut(s) 55, 82, 100
TaaI ACNGT 2 cut(s) 51, 268
TaqI TCGA 2 cut(s) 451, 597
TasI AATT 5 cut(s) 153, 354, 383, 441, 447
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 1 cut(s) 466
TseI GCWGC 3 cut(s) 167, 245, 550
TspDTI ATGAA 2 cut(s) 585, 620
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 1 cut(s) 466
Van91I CCANNNNNTGG 1 cut(s) 83
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 2 cut(s) 441, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.