Rh4BG121500

FLX-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002508
Physical Location & Seq
Reverse (-)
20337945 .. 20339476
1532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG121500.1

Sequence Viewer

Length: 957 bp
ATGGCTGGGCGGCGACATATTTTACCATCCTTTGACAGACGCCCTATACAGTCTCCAGGGATGATGCGGCACGGGCCATTGCCTGGGTTAGGTCGTGCTCCTGGCTACCGAGTTTTGGAGCCAGTACCCCGTGCTGAACTACTGGAAAACAAAATTGCATATCAAGCAGCAGAGATAAAACAACTTGCAGGAGACAATCATAGACTGGCAGCTACTGAGGTGGATTTGAGGCGGGAGCTTGTAGCTGCAGAGGAGGAAGTTCAAACAGTCAAGGCACACATGAGAAGCATCCAGACTGAGAGCGACATCCAGATCCGAGCGTTGCTTGATAAGATTGTAAAAAAGGAACAGGAAATTGCAGCCGGTGAGGGTGTGAAGAAGGAATTGCAAAAGGCTCACATGGAAGCACACAGCTTGGTGGAAGCACGGAAAGAGCTAAGAATTCAAATTCGACAGGCCACTGATGAGTTGCAGAAAGCTTGTATTGAGGTTAAGAAACTGCCAGATCTGCATGGTGAACTTGATAGTTTGAGACAAGAACACCAGAGGCTGCGTGCTACTTTTGAATATGAAAAAGGTAGAAACATAGAACAAGTCGAGCAAATGAAAGCAATGGAGAAGAATCTAATGGGCATGGCAGGAGAAGTAGAAAGGTTACGTGCTGAGGTCTTGAATGCAGAGAAGAGAGCACAAGGTGCTAGTGCGTATTTTGATGGTTATGGAAGGCCTTATGTTTCAATGGGTAATAGGCCACTGGGAGAGGGAATAATTCAATTTGCCAGCAGCAGCAGCAAGCAGCATGCTGCTGCAGGTATTCCAACTGCTGGGGATGGTGCTATCTGGGGAGCAGCATATAATCCCTTGCTTGGCACTGCTTCTGGTGGTGCTACTGTCCCTACCACTGGGAGCAGTGCTGTTTGGGGAGGAGCATATGATTCAGCACTTGCTCGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

318

Amino Acids

34.89

Weight (kDa)

8.51

Isoelectric Point (pI)

49.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LAMB4 PF24999 137 - 187 1.4e-06 Laminin subunit beta-4-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 800
AccB7I CCANNNNNTGG 2 cut(s) 83, 824
AciI CCGC 3 cut(s) 10, 67, 232
AclWI GGATC 1 cut(s) 307
AcsI RAATTY 2 cut(s) 441, 447
AcyI GRCGYC 1 cut(s) 40
AdeI CACNNNGTG 1 cut(s) 695
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 6 cut(s) 83, 89, 115, 824, 866, 902
AgsI TTSAA 6 cut(s) 263, 446, 566, 673, 738, 773
AjnI CCWGG 3 cut(s) 55, 82, 100
AluBI AGCT 6 cut(s) 212, 238, 245, 414, 436, 479
AluI AGCT 6 cut(s) 212, 238, 245, 414, 436, 479
Alw21I GWGCWC 2 cut(s) 100, 691
Alw26I GTCTC 3 cut(s) 57, 186, 526
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 2 cut(s) 215, 550
AoxI GGCC 4 cut(s) 74, 456, 725, 749
ApoI RAATTY 2 cut(s) 441, 447
AspS9I GGNCC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 377, 527
Bbv12I GWGCWC 2 cut(s) 100, 691
BbvCI CCTCAGC 1 cut(s) 663
BccI CCATC 3 cut(s) 34, 707, 824
BciT130I CCWGG 3 cut(s) 57, 84, 102
BcoDI GTCTC 3 cut(s) 57, 186, 526
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 2 cut(s) 246, 807
BfuAI ACCTGC 1 cut(s) 800
BglII AGATCT 1 cut(s) 505
Bme1390I CCNGG 3 cut(s) 57, 84, 102
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 57, 84, 102
BmrI ACTGGG 2 cut(s) 764, 912
BmsI GCATC 2 cut(s) 54, 297
BmuI ACTGGG 2 cut(s) 764, 912
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 663
BsaAI YACGTR 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 56, 83
Bsc4I CCNNNNNNNGG 6 cut(s) 83, 89, 115, 824, 866, 902
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 5 cut(s) 122, 147, 210, 759, 907
Bse3DI GCAATG 2 cut(s) 77, 618
BseBI CCWGG 3 cut(s) 57, 84, 102
BseDI CCNNGG 2 cut(s) 56, 83
BseGI GGATG 5 cut(s) 26, 66, 288, 306, 835
BseLI CCNNNNNNNGG 6 cut(s) 83, 89, 115, 824, 866, 902
BseMI GCAATG 2 cut(s) 77, 618
BseMII CTCAG 3 cut(s) 207, 288, 654
BseNI ACTGG 5 cut(s) 122, 147, 210, 759, 907
BseRI GAGGAG 2 cut(s) 266, 939
BseYI CCCAGC 2 cut(s) 5, 824
BshFI GGCC 4 cut(s) 76, 458, 727, 751
BsiHKAI GWGCWC 2 cut(s) 100, 691
BsiSI CCGG 1 cut(s) 363
BslFI GGGAC 1 cut(s) 878
BslI CCNNNNNNNGG 6 cut(s) 83, 89, 115, 824, 866, 902
BsmAI GTCTC 3 cut(s) 57, 186, 526
BsmFI GGGAC 1 cut(s) 878
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 4 cut(s) 76, 458, 727, 751
Bsp1286I GDGCHC 2 cut(s) 100, 691
Bsp143I GATC 2 cut(s) 312, 505
BspACI CCGC 3 cut(s) 10, 67, 232
BspANI GGCC 4 cut(s) 76, 458, 727, 751
BspCNI CTCAG 3 cut(s) 208, 289, 655
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 2 cut(s) 250, 811
BspMI ACCTGC 1 cut(s) 800
BspPI GGATC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 77, 618
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 5 cut(s) 122, 147, 210, 759, 907
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 2 cut(s) 56, 83
BssMI GATC 2 cut(s) 312, 505
BssNI GRCGYC 1 cut(s) 40
Bst2UI CCWGG 3 cut(s) 57, 84, 102
Bst4CI ACNGT 3 cut(s) 51, 268, 892
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 695
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 4 cut(s) 555, 781, 794, 801
BstDEI CTNAG 4 cut(s) 216, 297, 437, 663
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 5 cut(s) 26, 66, 288, 306, 835
BstKTI GATC 2 cut(s) 315, 508
BstMAI GTCTC 3 cut(s) 57, 186, 526
BstMBI GATC 2 cut(s) 312, 505
BstMWI GCNNNNNNNGC 5 cut(s) 73, 164, 508, 695, 789
BstNI CCWGG 3 cut(s) 57, 84, 102
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 3 cut(s) 55, 82, 100
BstSFI CTRYAG 2 cut(s) 246, 807
BstX2I RGATCY 2 cut(s) 312, 505
BstYI RGATCY 2 cut(s) 312, 505
BsuRI GGCC 4 cut(s) 76, 458, 727, 751
BtsCI GGATG 5 cut(s) 26, 66, 288, 306, 835
BtsI GCAGTG 2 cut(s) 870, 916
BtsIMutI CAGTG 5 cut(s) 459, 752, 870, 900, 916
BveI ACCTGC 1 cut(s) 800
Cac8I GCNNGC 4 cut(s) 555, 781, 794, 801
CaiI CAGNNNCTG 2 cut(s) 215, 550
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 74
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 125
CviAII CATG 5 cut(s) 280, 400, 512, 634, 800
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 4 cut(s) 216, 297, 437, 663
DpnI GATC 2 cut(s) 314, 507
DpnII GATC 2 cut(s) 312, 505
DraIII CACNNNGTG 1 cut(s) 695
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
Eco147I AGGCCT 1 cut(s) 727
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 441
EcoRII CCWGG 3 cut(s) 55, 82, 100
FaeI CATG 5 cut(s) 283, 403, 515, 637, 803
FaqI GGGAC 1 cut(s) 878
FatI CATG 5 cut(s) 279, 399, 511, 633, 799
FauI CCCGC 1 cut(s) 225
FauNDI CATATG 1 cut(s) 931
FokI GGATG 5 cut(s) 13, 73, 275, 293, 842
FspBI CTAG 1 cut(s) 699
GsaI CCCAGC 2 cut(s) 9, 828
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 4 cut(s) 76, 458, 727, 751
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 5 cut(s) 283, 403, 515, 637, 803
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 2 cut(s) 622, 935
HpaII CCGG 1 cut(s) 363
HphI GGTGA 2 cut(s) 377, 527
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 3 cut(s) 292, 310, 670
Hpy8I GTNNAC 1 cut(s) 518
HpyAV CCTTC 2 cut(s) 373, 717
HpyCH4III ACNGT 3 cut(s) 51, 268, 892
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 9 cut(s) 158, 188, 248, 359, 388, 472, 511, 677, 809
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 164, 508, 695, 789
HpyF3I CTNAG 4 cut(s) 216, 297, 437, 663
HpySE526I ACGT 1 cut(s) 658
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 5 cut(s) 283, 403, 515, 637, 803
Kzo9I GATC 2 cut(s) 312, 505
LmnI GCTCC 6 cut(s) 103, 118, 235, 845, 906, 926
LweI GCATC 2 cut(s) 54, 297
MaeI CTAG 1 cut(s) 699
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 1 cut(s) 654
MalI GATC 2 cut(s) 314, 507
MboI GATC 2 cut(s) 312, 505
MboII GAAGA 3 cut(s) 388, 631, 694
MflI RGATCY 2 cut(s) 312, 505
MhlI GDGCHC 2 cut(s) 100, 691
MluCI AATT 7 cut(s) 153, 354, 383, 441, 447, 768, 773
MmeI TCCRAC 1 cut(s) 842
MseI TTAA 1 cut(s) 492
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 3 cut(s) 57, 84, 102
Mva1269I GAATGC 1 cut(s) 679
MvaI CCWGG 3 cut(s) 57, 84, 102
MwoI GCNNNNNNNGC 5 cut(s) 73, 164, 508, 695, 789
NdeI CATATG 1 cut(s) 931
NdeII GATC 2 cut(s) 312, 505
NlaIII CATG 5 cut(s) 283, 403, 515, 637, 803
NlaIV GGNNCC 1 cut(s) 120
NspI RCATGY 1 cut(s) 803
PaeI GCATGC 1 cut(s) 803
PceI AGGCCT 1 cut(s) 727
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 2 cut(s) 622, 935
PflMI CCANNNNNTGG 2 cut(s) 83, 824
Ppu21I YACGTR 1 cut(s) 659
Psp6I CCWGG 3 cut(s) 55, 82, 100
PspFI CCCAGC 2 cut(s) 5, 824
PspGI CCWGG 3 cut(s) 55, 82, 100
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 2 cut(s) 250, 811
PstNI CAGNNNCTG 2 cut(s) 215, 550
PsuI RGATCY 2 cut(s) 312, 505
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 492
Sau3AI GATC 2 cut(s) 312, 505
Sau96I GGNCC 1 cut(s) 74
ScrFI CCNGG 3 cut(s) 57, 84, 102
SduI GDGCHC 2 cut(s) 100, 691
SfaNI GCATC 2 cut(s) 54, 297
SfcI CTRYAG 2 cut(s) 246, 807
SphI GCATGC 1 cut(s) 803
Sse9I AATT 7 cut(s) 153, 354, 383, 441, 447, 768, 773
SseBI AGGCCT 1 cut(s) 727
SsiI CCGC 3 cut(s) 10, 67, 232
SspMI CTAG 1 cut(s) 699
StuI AGGCCT 1 cut(s) 727
StyD4I CCNGG 3 cut(s) 55, 82, 100
TaaI ACNGT 3 cut(s) 51, 268, 892
TaiI ACGT 1 cut(s) 661
TaqI TCGA 2 cut(s) 451, 597
TasI AATT 7 cut(s) 153, 354, 383, 441, 447, 768, 773
TauI GCSGC 2 cut(s) 13, 70
TfiI GAWTC 2 cut(s) 622, 935
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 5 cut(s) 466, 759, 877, 907, 916
TspDTI ATGAA 2 cut(s) 585, 620
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 5 cut(s) 466, 759, 877, 907, 916
Van91I CCANNNNNTGG 2 cut(s) 83, 824
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 2 cut(s) 441, 447
XceI RCATGY 1 cut(s) 803
XspI CTAG 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.