Rw4G010350

FLX-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002508
Physical Location & Seq
Reverse (-)
23961292 .. 23963979
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010350.1

Sequence Viewer

Length: 1020 bp
ATGGCTGGGCGACGACATATTTTACCATCCTTTGACAGACGCCCTATACAGTCTCCAGGGATGATGCGGCACGGGCCATTGCCTGGGTTAGGTCATACTCCTGGCTACCGAGTTTTGGAGCCAGTACCCCGTGCTGAACTACTGGAAAACAAAATTGCATATCAAGCAGCAGAGATAAAACAACTTGCAGGAGACAATCACAGACTGGCAGCTACTGAGGTGGATTTGAGGCGGGAGCTTGTAGCTGCAGAGGAGGAAGTTCAAACAGTCAAGGCGCACATGAGAAGCATCCAGACTGAGAGCGACATCCAGATCCGAGCGTTGCTTGATAAGATTGTAAAAAAGGAACAGGAAATTGCAGCCGGTGAGGGTGTGAAGAAGGAATTGCAAAAGGCTCACATGGAAGCACACAGCTTGGTGGAAGCACGGAAAGAGCTAAGAATTCAAATTCAACAGGCCACTGATGAGTTGCAGAAAGCTTGTATTGAGGTTAAGAAACTGCCAGATCTGCATGGTGAACTTGATAGTTTGAGACAAGAACACCAGAGGCTGCGTGCTACTTTTGAATATGAAAAAGGTAGAAACATAGAACAAGTTGAGCAAATGAAAGCAATGGAGAAGAATCTAATGGGCATGGCAGGAGAAGTAGAAAGGTTGCGTGCTGAAGTCTTGAATGCAGAGAAGAGAGCACAAGCACCAAATCCACGTAATGGCGGTTACATGAATCCAGATTCTTATTACACTGCTACCATGCCAGGTGCTAGTGCGTATTTTGATGGTTATGGAAGGCCTTATGTTTCAATGGGTAATAGGCCACTGGGAGAGGGAATAATTCAATTTGCCAGCAGCAGCAGCAAGCAGCATGCTGCTGCAGGTATTCCAACTGCTGGGGATGGTGCTATCTGGGGAGCAGCATATAATCCCTTGCTTGGCACTGCTTCTGGTGGTGCTACTGTCCCTACCACTGGGAGCAGTGCTGTTTGGGGAGGAGCATATGATTCAGCACTTGCTCGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

37.17

Weight (kDa)

7.18

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LAMB4 PF24999 137 - 187 5.9e-06 Laminin subunit beta-4-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 863
AccB7I CCANNNNNTGG 3 cut(s) 83, 710, 887
AciI CCGC 3 cut(s) 67, 232, 714
AclWI GGATC 1 cut(s) 307
AcsI RAATTY 2 cut(s) 441, 447
AcuI CTGAAG 1 cut(s) 684
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 887, 929, 965
AgsI TTSAA 7 cut(s) 263, 446, 452, 566, 673, 801, 836
AjnI CCWGG 4 cut(s) 55, 82, 100, 754
AluBI AGCT 6 cut(s) 212, 238, 245, 414, 436, 479
AluI AGCT 6 cut(s) 212, 238, 245, 414, 436, 479
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 3 cut(s) 57, 186, 526
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 2 cut(s) 215, 550
AoxI GGCC 4 cut(s) 74, 456, 788, 812
ApoI RAATTY 2 cut(s) 441, 447
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 377, 527
Bbv12I GWGCWC 1 cut(s) 691
BccI CCATC 3 cut(s) 34, 770, 887
BciT130I CCWGG 4 cut(s) 57, 84, 102, 756
BcoDI GTCTC 3 cut(s) 57, 186, 526
BfaI CTAG 1 cut(s) 762
BfmI CTRYAG 2 cut(s) 246, 870
BfuAI ACCTGC 1 cut(s) 863
BglII AGATCT 1 cut(s) 505
Bme1390I CCNGG 4 cut(s) 57, 84, 102, 756
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 4 cut(s) 57, 84, 102, 756
BmrI ACTGGG 2 cut(s) 827, 975
BmsI GCATC 2 cut(s) 54, 297
BmuI ACTGGG 2 cut(s) 827, 975
BpmI CTGGAG 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 707
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 56, 83
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 887, 929, 965
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 5 cut(s) 122, 147, 210, 822, 970
Bse3DI GCAATG 2 cut(s) 77, 618
BseBI CCWGG 4 cut(s) 57, 84, 102, 756
BseDI CCNNGG 2 cut(s) 56, 83
BseGI GGATG 5 cut(s) 26, 66, 288, 306, 898
BseLI CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 887, 929, 965
BseMI GCAATG 2 cut(s) 77, 618
BseMII CTCAG 2 cut(s) 207, 288
BseNI ACTGG 5 cut(s) 122, 147, 210, 822, 970
BseRI GAGGAG 2 cut(s) 266, 1002
BseYI CCCAGC 2 cut(s) 5, 887
BshFI GGCC 4 cut(s) 76, 458, 790, 814
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 1 cut(s) 363
BslFI GGGAC 1 cut(s) 941
BslI CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 887, 929, 965
BsmAI GTCTC 3 cut(s) 57, 186, 526
BsmFI GGGAC 1 cut(s) 941
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 4 cut(s) 76, 458, 790, 814
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 2 cut(s) 312, 505
BspACI CCGC 3 cut(s) 67, 232, 714
BspANI GGCC 4 cut(s) 76, 458, 790, 814
BspCNI CTCAG 2 cut(s) 208, 289
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 2 cut(s) 250, 874
BspMI ACCTGC 1 cut(s) 863
BspPI GGATC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 77, 618
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 5 cut(s) 122, 147, 210, 822, 970
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 2 cut(s) 56, 83
BssMI GATC 2 cut(s) 312, 505
BssNI GRCGYC 1 cut(s) 40
Bst2UI CCWGG 4 cut(s) 57, 84, 102, 756
Bst4CI ACNGT 3 cut(s) 51, 268, 955
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 1 cut(s) 40
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 5 cut(s) 555, 660, 844, 857, 864
BstDEI CTNAG 3 cut(s) 216, 297, 437
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 5 cut(s) 26, 66, 288, 306, 898
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 2 cut(s) 315, 508
BstMAI GTCTC 3 cut(s) 57, 186, 526
BstMBI GATC 2 cut(s) 312, 505
BstMWI GCNNNNNNNGC 4 cut(s) 73, 164, 508, 852
BstNI CCWGG 4 cut(s) 57, 84, 102, 756
BstNSI RCATGY 1 cut(s) 866
BstSCI CCNGG 4 cut(s) 55, 82, 100, 754
BstSFI CTRYAG 2 cut(s) 246, 870
BstX2I RGATCY 2 cut(s) 312, 505
BstYI RGATCY 2 cut(s) 312, 505
BsuRI GGCC 4 cut(s) 76, 458, 790, 814
BtsCI GGATG 5 cut(s) 26, 66, 288, 306, 898
BtsI GCAGTG 3 cut(s) 741, 933, 979
BtsIMutI CAGTG 6 cut(s) 459, 741, 815, 933, 963, 979
BveI ACCTGC 1 cut(s) 863
Cac8I GCNNGC 5 cut(s) 555, 660, 844, 857, 864
CaiI CAGNNNCTG 2 cut(s) 215, 550
CfoI GCGC 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 74
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 125
CviAII CATG 7 cut(s) 280, 400, 512, 634, 721, 751, 863
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 3 cut(s) 216, 297, 437
DpnI GATC 2 cut(s) 314, 507
DpnII GATC 2 cut(s) 312, 505
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
Eco147I AGGCCT 1 cut(s) 790
Eco57I CTGAAG 1 cut(s) 684
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 441
EcoRII CCWGG 4 cut(s) 55, 82, 100, 754
FaeI CATG 7 cut(s) 283, 403, 515, 637, 724, 754, 866
FaqI GGGAC 1 cut(s) 941
FatI CATG 7 cut(s) 279, 399, 511, 633, 720, 750, 862
FauI CCCGC 1 cut(s) 225
FauNDI CATATG 1 cut(s) 994
FokI GGATG 5 cut(s) 13, 73, 275, 293, 905
FspBI CTAG 1 cut(s) 762
GlaI GCGC 1 cut(s) 276
GsaI CCCAGC 2 cut(s) 9, 891
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 4 cut(s) 76, 458, 790, 814
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 48
HhaI GCGC 1 cut(s) 277
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 7 cut(s) 283, 403, 515, 637, 724, 754, 866
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 4 cut(s) 622, 724, 731, 998
HpaII CCGG 1 cut(s) 363
HphI GGTGA 2 cut(s) 377, 527
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 4 cut(s) 292, 310, 670, 728
Hpy8I GTNNAC 1 cut(s) 518
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 2 cut(s) 373, 780
HpyCH4III ACNGT 3 cut(s) 51, 268, 955
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 9 cut(s) 158, 188, 248, 359, 388, 472, 511, 677, 872
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 164, 508, 852
HpyF3I CTNAG 3 cut(s) 216, 297, 437
HpySE526I ACGT 1 cut(s) 706
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 7 cut(s) 283, 403, 515, 637, 724, 754, 866
HspAI GCGC 1 cut(s) 275
Kzo9I GATC 2 cut(s) 312, 505
LmnI GCTCC 5 cut(s) 118, 235, 908, 969, 989
LweI GCATC 2 cut(s) 54, 297
MaeI CTAG 1 cut(s) 762
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 1 cut(s) 716
MalI GATC 2 cut(s) 314, 507
MboI GATC 2 cut(s) 312, 505
MboII GAAGA 3 cut(s) 388, 631, 694
MflI RGATCY 2 cut(s) 312, 505
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 7 cut(s) 153, 354, 383, 441, 447, 831, 836
MmeI TCCRAC 1 cut(s) 905
MnlI CCTC 9 cut(s) 211, 222, 244, 247, 361, 481, 540, 817, 980
MseI TTAA 1 cut(s) 492
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 4 cut(s) 57, 84, 102, 756
Mva1269I GAATGC 1 cut(s) 679
MvaI CCWGG 4 cut(s) 57, 84, 102, 756
MwoI GCNNNNNNNGC 4 cut(s) 73, 164, 508, 852
NdeI CATATG 1 cut(s) 994
NdeII GATC 2 cut(s) 312, 505
NlaIII CATG 7 cut(s) 283, 403, 515, 637, 724, 754, 866
NlaIV GGNNCC 1 cut(s) 120
NspI RCATGY 1 cut(s) 866
PaeI GCATGC 1 cut(s) 866
PceI AGGCCT 1 cut(s) 790
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 4 cut(s) 622, 724, 731, 998
PflMI CCANNNNNTGG 3 cut(s) 83, 710, 887
Ppu21I YACGTR 1 cut(s) 707
Psp6I CCWGG 4 cut(s) 55, 82, 100, 754
PspFI CCCAGC 2 cut(s) 5, 887
PspGI CCWGG 4 cut(s) 55, 82, 100, 754
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 2 cut(s) 250, 874
PstNI CAGNNNCTG 2 cut(s) 215, 550
PsuI RGATCY 2 cut(s) 312, 505
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 492
Sau3AI GATC 2 cut(s) 312, 505
Sau96I GGNCC 1 cut(s) 74
ScrFI CCNGG 4 cut(s) 57, 84, 102, 756
SduI GDGCHC 1 cut(s) 691
SfaNI GCATC 2 cut(s) 54, 297
SfcI CTRYAG 2 cut(s) 246, 870
SphI GCATGC 1 cut(s) 866
Sse9I AATT 7 cut(s) 153, 354, 383, 441, 447, 831, 836
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 3 cut(s) 67, 232, 714
SspMI CTAG 1 cut(s) 762
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 4 cut(s) 55, 82, 100, 754
TaaI ACNGT 3 cut(s) 51, 268, 955
TaiI ACGT 1 cut(s) 709
TasI AATT 7 cut(s) 153, 354, 383, 441, 447, 831, 836
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 4 cut(s) 622, 724, 731, 998
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 6 cut(s) 466, 748, 822, 940, 970, 979
TspDTI ATGAA 3 cut(s) 585, 620, 737
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 6 cut(s) 466, 748, 822, 940, 970, 979
Van91I CCANNNNNTGG 3 cut(s) 83, 710, 887
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 2 cut(s) 441, 447
XceI RCATGY 1 cut(s) 866
XspI CTAG 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.