FvH4_4g12380

mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005851
Physical Location & Seq
Forward (+)
16087259 .. 16098476
11218 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12380.t1

Sequence Viewer

Length: 4272 bp
ATGGCTGGAAAATCAAACAAAGGGAGGAACCGGAGAGCGTCGAACAATGCTGCAAATTCATCAGCTGAGGTGGTGATCCAATCCGACGCTCCTGTGAAAGACAGCTCAAAAGATGACTCTGAGTCCATCAACACCAATGCTAATGGTGTTCCGACTGTGCAAGAGTGGGCTAGTGACAAGGTGGCTAGTGAAAAGGTGGAGGTAAAGGATTCTGAAACTGATGTCGCAGGCGGCGAAGCAAAGCAAGGTGAATCTGAAACAGAAAATTCAGCTGGCCAACCTAAGCAAGGTGACCTTCATCTTTACCCTGTCTGTGTCAAGACACAAAGTGGCGAGAAGCTAGAACTACAATTGAACCCAGGAGATTCTGTTATGGATATAAGACAATTTCTGCTTGATGCTCCTGAGACATGCTTCTTTACATGCTATGACTTGTTGCTGCACACCAAAGATGGCTCAACCTATCATTTGGAAGATTTTAATGAAATCTCTGAAGTTGCTGACATTACTATTGGTGGATGCTTCTTAGAAATGGTTCCAGCTTTATATGATGATAGATCTATAAGGGCTCATGTTCATCGTACCAGAGAATTACTCTCACTTTCGACACTTCATGCGTCACTATCAACATCACTTGCATTACAATATGAGACAGCTCAGAACAAAGTAGCAACTACTGAAGACTCCGTCAAAACTGAGGTCCCTGAGCTGGATGGTTTGGGCTTTATGGATGATGTTGCCGGTTCGAGTAATTTGCTCTCATCACCCTCAAAAGAGATCAAATGTGTAGAGAGTATTGTGTTTTCATCCTTTAATCCTCCACCAAGCTACAGAAGGCTTGTTGGTGACCTGATTTATCTGGATGTGGTTACATTGGAGGGTAATAAACTTTGTATTACGGGGACTACAAAAATGTTCTATGTCAATTCAAGCTCCGTGAATAATACCCTTAATCCCAGGCCTAGCAAAACTTATCCGGAAGCGACCACTCTTGTTGGGATTTTGCAAAAGATCAGCTCTAAGTTCCAAAAAGCTTTTTGTGAAATTTTGGAGCGGAGAGCTTCTGCACATCCTTTTGAAAATGTGCAATCTCTCTTACCACCAAATTCATGGCTAGGGTTACATCCGGTTCCTGATCACAAGCGCGATGCAGCTAGAGCAGAGGATGCATTGACCCTCTCCTATGGTAGTGAGCTAATTGGTATGCAAAGGGATTGGAATGAGGAGTTGCAATCTTGTAGGGAATTTCCGCATACAACTCCTCAGGAAAGAATTTTGCGGGATAGAGCTCTGTACAAAGTGACATCAGATTTCGTGGATGCAGCAATTAGTGGTGCTACTGGAGTTATCAGCAGATGCATACCACCTATAAATCCAACTGATCCTGAGTGCTTCCACATGTATGTTCACAATAACATATTCTTCAGTTTCGCTGTTGATGCGGATCTTGAGCAGCTGTCCAAGAACCATATGTCAGATTCCAATTCGAAGATGGGGAGCACTGGTTCTCTACGCAGTTCTTCTGAAATGTCTACAGGCAGTTTGTTGCATAAAGAGAGTGAAATTCTCGGTGAAAAATGTGATGCGTCATGCGCAGGGGAATGCCATGATGCCATGGAGACACAATTGGGCGAGACTGAGCAGGCTACTTATGCTTCTGCCAATAATGATCTGAAAGGCACTAAAGCATACCAGGAAGCAGATGTCCCTGGGCTTTATAATCTTGCTATGGCCATAATCGATTACAGAGGCCATAGAGTAGTAGCTCAGAGTGTTTTACCAGGCATTCTTCAAGGGGATAAATCAGACTCTCTTCTGTATGGTTCTGTCGACAATGGCAAGAAGATATGCTGGAATGAGGAGTTTCATTCCAAGGTGGTGGAGGCTGCTAAACGCCTTCATTTGAAGGAACACACTGTCCGTGATGGATCTGGAAATGTTTTCAAATTAGCTGCTCCAGTCGAATGCAAGGGTATTGTTGGTAGTGATGACAGGCATTATCTTCTTGACTTGATGAGAGTAACTCCTCGTGATGCAAACTTCACTGGGTCTGGGTCTCGATTTTGTATCTTGAGGCCAGAACTAATTACTTCTTATTGCCAGGTCCTTGATGCAGAGAAATCAAAATCTAAGTCTATCTGTGAAGGAGAGGCTCAGGTCACCACTGATGGGCCAAATGGTAACCAGGACATAATAGAAAAGGAGAAGATCAGTAATGCTGAAGAAATTGTCTCTCCACCAGCTGAAATTTCTGAACCGCGTGAGGAAATTCTTTTCAACCCTAATGTCTTCACTGAGTTTAAGTTGGCTGGAAGCGCAGAGGAGATAGCAACAGATGAAGAAAATGTGAGGAAAGCCAGTTCGTATCTTACTGACGTTGTTCTTCCCAAGTTCATACAAGATCTTTGCACACTTGAAGTATCACCCATGGATGGGCAGACACTAACCGAAGCTCTCCATGCTCATGGAATTAATGTTCGGTATATCGGAAAAGTGGCTGAAGGGACCAGGCATTTACCTCATCTTTGGGATCTTTGTTCTAATGAGATTGTGGTTAGATCAGCTAAGCACATTCTCAAGGATGTCTTAAGAGACACGGAGGATCATGATATTGGGCCTGCAATCTGCCATTTCTTCAACTGTTTTTTCGGAAGCAATCAAGCTGTTGGCTCAAAAGTCACTGCTAATAGTTCTCAATCTAGAATCCCTAAAAAAGAACAAGCAGGTCATCAATCTCCTGGGAAACGGTCAAAAGGACAAGGAAGATGGAAGGGTGGAGCTTCTACGAAAAAGAATATATCGTCATATATGGATGTTAGCTCAGAGATTCTATGGTCTGACATTCAAGAATTTGCCAAACTCAAATATGAGTTCGAGTTGCCAAAGGATGCTAGGACGCATGTAAAGAAAGATTCAGTTATCCGTAATCTCTGCCAAAAGGTTGGCATTACAATTGCAGCTCGCAGATATGATCTCAATTCTGCTGCACCTTTTGAAATATCAGATATATTGAATCTTCAACCTGTAGTGAAGCACTCAGTTCCTGTTTGTTCAGAAGCCAAGGAACTCGTGGAAACAGGGAAAATCCAATTGGCTGAGGGAATGTTGAGTGAAGCCTACACAGTATTTTCTGAGGCCTTCTCCATACTGCAGCAGGTTACAGGCCCAATGCATCGAGAGGTTGCTAACTGCTGTCGGTATCTTGCAATGGTTCTGTATCATGCAGGAGACATGGCAGGGGCCATAATGCAACAGCACAAGGAGCTAATTATAAATGAGCGTTGCCTGGGTTTGGACCACCCTGACACTGCTCACAGCTATGGGAATATGGCTCTTTTCTATCATGGACTTAACCAAACAGAACTTGCCCTACGACATATGTCCCGAGCATTGCTCTTGCTAAGTTTGTCATCTGGCCCAGATCACCCTGATGTTGCAGCAACATTTATTAATGTCGCTATGATGTACCAGGATCTTGGCAAGATGCCAACTGCTCTTCGATATTTGCAAGAGGCTTTGAAAAAGAATGAAAGGCTTCTTGGCGTGGAACATATCCAGACTGCTGTATGCTATCATGCACTTGCCATTGCATGTAATTGTATGGGCGCCTTCAAGCTCTCCCACCAGCATGAGAAGAAAACTTATGATATACTTGTCAAACAATTGGGTGAGGAGGACTCGAGGACACGCGACTCTCAAAACTGGATGAAGACATTTAAGTTGCGAGAGCAACAGATGAATGCACAAAAGCAAAAAGGTCAAGCTCTGAATGCTGCTCAAGCTCAAAAGGCAATTGACATTCTAAAGGCCAATCCTGACTGGGCGCAGGCACTTCAATCTGCTGCTATAGCCGGCGGGTCTGGGAGTTCCAACGCTTCTGTGAACAGATCACTTAATACTGCTGCAATAATGGGTGAAGCTTTTCCCCGAGGGAGGGGAGTTGATGAAAGGGCTGCACGAGCAGCAGCTGCCAACAGAAAGAAGGCAGCAGCAAGGGGCCTTTTACGTCCACACAGTGTTCCTGTCCAAGCATATAATCCACTGACTCAGATCATCAATATGATGAGTGCTGGTGGGGCCCCAGGGTCAGCGCAAAATGGAGAAACTAATGGATCGAGGGAAGCCAACAACCATCCTTCAAATGGCCCGGCAGATGCGAAAACTAACCAACCATCATTAGAGCAGGAAGGTCAACCTCCAGTTGGGTTGGGCAAAGGCTTGGCAGCACTTGATGGCAAGAAACAAAAATCAAAAGCCAAAGCTGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N DUF727 TPR_10 TPR_12 eIF3_p135

Protein Analysis

1424

Amino Acids

155.62

Weight (kDa)

5.89

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 122 - 198 3.3e-13 Mitochondrial function, CLU-N-term
CLU PF13236 405 - 681 3.7e-75 Clustered mitochondria
eIF3_p135 PF12807 807 - 991 5.1e-48 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 1068 - 1131 1.1e-08 Tetratricopeptide repeat
TPR_12 PF13424 1102 - 1174 2.8e-12 Tetratricopeptide repeat
TPR_NPHP3 PF24885 1104 - 1225 4.2e-11 Nephrocystin-3 TPR domain
TPR_12 PF13424 1144 - 1217 5.5e-13 Tetratricopeptide repeat
TPR_10 PF13374 1145 - 1185 7.8e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1719, 3272
Acc16I TGCGCA 1 cut(s) 1594
Acc36I ACCTGC 2 cut(s) 2714, 3145
AccB1I GGYRCC 1 cut(s) 3605
AccBSI CCGCTC 1 cut(s) 1054
AccI GTMKAC 2 cut(s) 1532, 1830
AccII CGCG 3 cut(s) 1146, 2260, 3690
AccIII TCCGGA 1 cut(s) 976
AciI CCGC 7 cut(s) 231, 1054, 1250, 1279, 1442, 2258, 3855
AclWI GGATC 8 cut(s) 70, 1376, 1452, 1936, 2538, 2610, 3480, 4120
AcoI YGGCCR 2 cut(s) 274, 1731
AcuI CTGAAG 5 cut(s) 513, 699, 1408, 2241, 2520
AcyI GRCGYC 1 cut(s) 3606
AdeI CACNNNGTG 2 cut(s) 329, 4016
AfaI GTAC 3 cut(s) 583, 1295, 3467
AflII CTTAAG 1 cut(s) 2587
AflIII ACRYGT 1 cut(s) 1398
AhdI GACNNNNNGTC 1 cut(s) 121
AjuI GAANNNNNNNTTGG 4 cut(s) 3074, 3106, 3522, 3554
AloI GAACNNNNNNTCC 8 cut(s) 1489, 1521, 1902, 1934, 2344, 2376, 2460, 2492
Alw21I GWGCWC 2 cut(s) 1291, 1502
Alw26I GTCTC 8 cut(s) 401, 644, 1613, 1628, 2061, 2236, 2586, 3222
AlwI GGATC 8 cut(s) 70, 1376, 1452, 1936, 2538, 2610, 3480, 4120
AlwNI CAGNNNCTG 2 cut(s) 3044, 3968
Ama87I CYCGRG 3 cut(s) 3384, 3679, 3927
Aor13HI TCCGGA 1 cut(s) 976
ApaI GGGCCC 1 cut(s) 4081
ArsI GACNNNNNNTTYG 4 cut(s) 684, 716, 2117, 2149
AseI ATTAAT 2 cut(s) 2472, 3450
Asp700I GAANNNNTTC 3 cut(s) 534, 3534, 3921
AspLEI GCGC 6 cut(s) 1146, 1595, 2318, 3608, 3826, 4093
AsuC2I CCSGG 1 cut(s) 4148
AsuII TTCGAA 1 cut(s) 1487
AvaI CYCGRG 3 cut(s) 3384, 3679, 3927
AvaII GGWCC 4 cut(s) 700, 2104, 2505, 3295
AxyI CCTNAGG 1 cut(s) 1263
BaeGI GKGCMC 1 cut(s) 4081
BalI TGGCCA 2 cut(s) 276, 1733
BanI GGYRCC 1 cut(s) 3605
BanII GRGCYC 3 cut(s) 571, 1291, 4081
BauI CACGAG 3 cut(s) 2028, 3068, 3957
BbsI GAAGAC 3 cut(s) 687, 2281, 3716
Bbv12I GWGCWC 2 cut(s) 1291, 1502
BbvCI CCTCAGC 2 cut(s) 66, 3096
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BclI TGATCA 1 cut(s) 1135
BcnI CCSGG 1 cut(s) 4148
BcoDI GTCTC 8 cut(s) 401, 644, 1613, 1628, 2061, 2236, 2586, 3222
BfaI CTAG 8 cut(s) 171, 186, 341, 963, 1115, 1155, 2700, 2892
BfmI CTRYAG 5 cut(s) 829, 1533, 3024, 3149, 3846
BfoI RGCGCY 1 cut(s) 3609
BfrI CTTAAG 1 cut(s) 2587
BfuAI ACCTGC 2 cut(s) 2714, 3145
BglII AGATCT 2 cut(s) 557, 2401
BlpI GCTNAGC 1 cut(s) 2565
Bme18I GGWCC 4 cut(s) 700, 2104, 2505, 3295
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 3 cut(s) 3384, 3679, 3927
BmrI ACTGGG 2 cut(s) 2055, 3829
BmuI ACTGGG 2 cut(s) 2055, 3829
BoxI GACNNNNGTC 1 cut(s) 3379
BpiI GAAGAC 3 cut(s) 687, 2281, 3716
BpmI CTGGAG 3 cut(s) 1362, 1941, 4182
Bpu10I CCTNAGC 5 cut(s) 66, 282, 705, 2154, 3096
Bpu1102I GCTNAGC 1 cut(s) 2565
Bpu14I TTCGAA 1 cut(s) 1487
BpuEI CTTGAG 4 cut(s) 1469, 2092, 2561, 3762
BpuMI CCSGG 1 cut(s) 4148
Bsa29I ATCGAT 1 cut(s) 1740
BsaBI GATNNNNATC 1 cut(s) 1443
BsaHI GRCGYC 1 cut(s) 3606
BsaI GGTCTC 1 cut(s) 2061
BsaWI WCCGGW 3 cut(s) 30, 976, 1126
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 1489, 1519
Bse118I RCCGGY 2 cut(s) 740, 3851
Bse1I ACTGG 8 cut(s) 1345, 1507, 1958, 2050, 2358, 3707, 3824, 4199
Bse21I CCTNAGG 1 cut(s) 1263
Bse3DI GCAATG 3 cut(s) 3213, 3389, 3585
Bse8I GATNNNNATC 1 cut(s) 1443
BseAI TCCGGA 1 cut(s) 976
BseCI ATCGAT 1 cut(s) 1740
BseJI GATNNNNATC 1 cut(s) 1443
BseMI GCAATG 3 cut(s) 3213, 3389, 3585
BseNI ACTGG 8 cut(s) 1345, 1507, 1958, 2050, 2358, 3707, 3824, 4199
BseRI GAGGAG 6 cut(s) 1238, 1251, 1874, 2016, 2336, 3686
BseSI GKGCMC 1 cut(s) 4081
BsgI GTGCAG 4 cut(s) 425, 1050, 2970, 3939
Bsh1236I CGCG 3 cut(s) 1146, 2260, 3690
BshNI GGYRCC 1 cut(s) 3605
BshVI ATCGAT 1 cut(s) 1740
BsiHKAI GWGCWC 2 cut(s) 1291, 1502
BsiHKCI CYCGRG 3 cut(s) 3384, 3679, 3927
BsiSI CCGG 6 cut(s) 31, 741, 977, 1127, 3852, 4148
BslFI GGGAC 5 cut(s) 686, 916, 1691, 2518, 3367
BsmAI GTCTC 8 cut(s) 401, 644, 1613, 1628, 2061, 2236, 2586, 3222
BsmFI GGGAC 5 cut(s) 686, 916, 1691, 2518, 3367
BsmI GAATGC 5 cut(s) 1607, 1785, 1970, 3745, 3775
Bso31I GGTCTC 1 cut(s) 2061
BsoBI CYCGRG 3 cut(s) 3384, 3679, 3927
Bsp119I TTCGAA 1 cut(s) 1487
Bsp120I GGGCCC 1 cut(s) 4077
Bsp1286I GDGCHC 4 cut(s) 571, 1291, 1502, 4081
Bsp13I TCCGGA 1 cut(s) 976
Bsp1407I TGTACA 1 cut(s) 1293
Bsp1720I GCTNAGC 1 cut(s) 2565
Bsp19I CCATGG 2 cut(s) 1614, 2427
BspACI CCGC 7 cut(s) 231, 1054, 1250, 1279, 1442, 2258, 3855
BspDI ATCGAT 1 cut(s) 1740
BspEI TCCGGA 1 cut(s) 976
BspFNI CGCG 3 cut(s) 1146, 2260, 3690
BspHI TCATGA 2 cut(s) 2605, 4268
BspMAI CTGCAG 1 cut(s) 3153
BspMI ACCTGC 2 cut(s) 2714, 3145
BspPI GGATC 8 cut(s) 70, 1376, 1452, 1936, 2538, 2610, 3480, 4120
BspQI GCTCTTC 1 cut(s) 3501
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 1 cut(s) 3605
BspTI CTTAAG 1 cut(s) 2587
BspTNI GGTCTC 1 cut(s) 2061
BsrBI CCGCTC 1 cut(s) 1054
BsrDI GCAATG 3 cut(s) 3213, 3389, 3585
BsrFI RCCGGY 2 cut(s) 740, 3851
BsrGI TGTACA 1 cut(s) 1293
BsrI ACTGG 8 cut(s) 1345, 1507, 1958, 2050, 2358, 3707, 3824, 4199
BssAI RCCGGY 2 cut(s) 740, 3851
BssNI GRCGYC 1 cut(s) 3606
BssSI CACGAG 3 cut(s) 2028, 3068, 3957
BssT1I CCWWGG 4 cut(s) 1614, 1872, 2427, 3060
Bst2BI CACGAG 3 cut(s) 2028, 3068, 3957
Bst4CI ACNGT 6 cut(s) 157, 1918, 2642, 2748, 3124, 4016
Bst6I CTCTTC 2 cut(s) 1818, 3501
BstACI GRCGYC 1 cut(s) 3606
BstAFI CTTAAG 1 cut(s) 2587
BstAPI GCANNNNNTGC 2 cut(s) 1166, 3968
BstAUI TGTACA 1 cut(s) 1293
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 7 cut(s) 229, 274, 1644, 2619, 2962, 3828, 3853
BstDSI CCRYGG 2 cut(s) 1614, 2427
BstEII GGTNACC 4 cut(s) 290, 845, 2158, 2180
BstENI CCTNNNNNAGG 1 cut(s) 285
BstFNI CGCG 3 cut(s) 1146, 2260, 3690
BstH2I RGCGCY 1 cut(s) 3609
BstHHI GCGC 6 cut(s) 1146, 1595, 2318, 3608, 3826, 4093
BstMAI GTCTC 8 cut(s) 401, 644, 1613, 1628, 2061, 2236, 2586, 3222
BstNSI RCATGY 5 cut(s) 414, 426, 1402, 2903, 3594
BstPAI GACNNNNGTC 1 cut(s) 3379
BstPI GGTNACC 4 cut(s) 290, 845, 2158, 2180
BstSFI CTRYAG 5 cut(s) 829, 1533, 3024, 3149, 3846
BstSLI GKGCMC 1 cut(s) 4081
BstUI CGCG 3 cut(s) 1146, 2260, 3690
BstV2I GAAGAC 3 cut(s) 687, 2281, 3716
BstX2I RGATCY 6 cut(s) 557, 1444, 1928, 2401, 2530, 3472
BstXI CCANNNNNNTGG 5 cut(s) 1110, 1879, 2465, 2942, 3476
BstYI RGATCY 6 cut(s) 557, 1444, 1928, 2401, 2530, 3472
Bsu15I ATCGAT 1 cut(s) 1740
Bsu36I CCTNAGG 1 cut(s) 1263
BsuTUI ATCGAT 1 cut(s) 1740
BtgI CCRYGG 2 cut(s) 1614, 2427
BtgZI GCGATG 1 cut(s) 1161
BtsI GCAGTG 2 cut(s) 2679, 3306
BtsIMutI CAGTG 9 cut(s) 1500, 1914, 2043, 2163, 2292, 2679, 3306, 4021, 4040
BveI ACCTGC 2 cut(s) 2714, 3145
Cac8I GCNNGC 7 cut(s) 229, 274, 1644, 2619, 2962, 3828, 3853
CaiI CAGNNNCTG 2 cut(s) 3044, 3968
CciI TCATGA 2 cut(s) 2605, 4268
CfoI GCGC 6 cut(s) 1146, 1595, 2318, 3608, 3826, 4093
Cfr10I RCCGGY 2 cut(s) 740, 3851
ClaI ATCGAT 1 cut(s) 1740
CseI GACGC 5 cut(s) 27, 95, 606, 1575, 2905
Csp6I GTAC 3 cut(s) 582, 1294, 3466
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 3 cut(s) 582, 1294, 3466
DinI GGCGCC 1 cut(s) 3607
DraIII CACNNNGTG 2 cut(s) 329, 4016
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 2 cut(s) 274, 1731
Eam1104I CTCTTC 2 cut(s) 1818, 3501
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 2 cut(s) 1818, 3501
Ecl136II GAGCTC 1 cut(s) 1289
Eco130I CCWWGG 4 cut(s) 1614, 1872, 2427, 3060
Eco147I AGGCCT 2 cut(s) 961, 3137
Eco24I GRGCYC 3 cut(s) 571, 1291, 4081
Eco31I GGTCTC 1 cut(s) 2061
Eco47I GGWCC 4 cut(s) 700, 2104, 2505, 3295
Eco53kI GAGCTC 1 cut(s) 1289
Eco57I CTGAAG 5 cut(s) 513, 699, 1408, 2241, 2520
Eco81I CCTNAGG 1 cut(s) 1263
Eco88I CYCGRG 3 cut(s) 3384, 3679, 3927
Eco91I GGTNACC 4 cut(s) 290, 845, 2158, 2180
EcoICRI GAGCTC 1 cut(s) 1289
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoO109I RGGNCCY 5 cut(s) 700, 2104, 3997, 4077, 4078
EcoO65I GGTNACC 4 cut(s) 290, 845, 2158, 2180
EcoT14I CCWWGG 4 cut(s) 1614, 1872, 2427, 3060
EcoT22I ATGCAT 3 cut(s) 1171, 1361, 3174
EcoT38I GRGCYC 3 cut(s) 571, 1291, 4081
EgeI GGCGCC 1 cut(s) 3607
EheI GGCGCC 1 cut(s) 3607
ErhI CCWWGG 4 cut(s) 1614, 1872, 2427, 3060
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 2570, 2602
FaqI GGGAC 5 cut(s) 686, 916, 1691, 2518, 3367
FauI CCCGC 2 cut(s) 1272, 3848
FauNDI CATATG 2 cut(s) 1470, 3378
FbaI TGATCA 1 cut(s) 1135
FblI GTMKAC 2 cut(s) 1532, 1830
FriOI GRGCYC 3 cut(s) 571, 1291, 4081
FspBI CTAG 8 cut(s) 171, 186, 341, 963, 1115, 1155, 2700, 2892
FspI TGCGCA 1 cut(s) 1594
GlaI GCGC 6 cut(s) 1145, 1594, 2317, 3607, 3825, 4092
GsuI CTGGAG 3 cut(s) 1362, 1941, 4182
HaeII RGCGCY 1 cut(s) 3609
HapII CCGG 6 cut(s) 31, 741, 977, 1127, 3852, 4148
HgaI GACGC 5 cut(s) 27, 95, 606, 1575, 2905
HhaI GCGC 6 cut(s) 1146, 1595, 2318, 3608, 3826, 4093
Hin1I GRCGYC 1 cut(s) 3606
Hin6I GCGC 6 cut(s) 1144, 1593, 2316, 3606, 3824, 4091
HinP1I GCGC 6 cut(s) 1144, 1593, 2316, 3606, 3824, 4091
HincII GTYRAC 2 cut(s) 1831, 4193
HindII GTYRAC 2 cut(s) 1831, 4193
HindIII AAGCTT 2 cut(s) 1032, 3918
HpaII CCGG 6 cut(s) 31, 741, 977, 1127, 3852, 4148
Hpy166II GTNNAC 6 cut(s) 1408, 1533, 1831, 3883, 4010, 4193
Hpy8I GTNNAC 6 cut(s) 1408, 1533, 1831, 3883, 4010, 4193
Hpy99I CGWCG 2 cut(s) 43, 89
HpyCH4III ACNGT 6 cut(s) 157, 1918, 2642, 2748, 3124, 4016
HpyCH4IV ACGT 2 cut(s) 2376, 4006
HpySE526I ACGT 2 cut(s) 2376, 4006
Hsp92I GRCGYC 1 cut(s) 3606
HspAI GCGC 6 cut(s) 1144, 1593, 2316, 3606, 3824, 4091
KasI GGCGCC 1 cut(s) 3605
Kpn2I TCCGGA 1 cut(s) 976
KroI GCCGGC 1 cut(s) 3851
KroNI GCCGGC 1 cut(s) 3853
Ksp22I TGATCA 1 cut(s) 1135
LguI GCTCTTC 1 cut(s) 3501
LmnI GCTCC 8 cut(s) 94, 406, 938, 1051, 1497, 1960, 2777, 3262
MaeI CTAG 8 cut(s) 171, 186, 341, 963, 1115, 1155, 2700, 2892
MaeII ACGT 2 cut(s) 2376, 4006
MbiI CCGCTC 1 cut(s) 1054
MfeI CAATTG 6 cut(s) 350, 1625, 2952, 3089, 3662, 3792
MflI RGATCY 6 cut(s) 557, 1444, 1928, 2401, 2530, 3472
MhlI GDGCHC 4 cut(s) 571, 1291, 1502, 4081
MlsI TGGCCA 2 cut(s) 276, 1733
MluNI TGGCCA 2 cut(s) 276, 1733
Mly113I GGCGCC 1 cut(s) 3606
MlyI GAGTC 7 cut(s) 110, 131, 677, 1802, 3671, 3686, 4039
MmeI TCCRAC 4 cut(s) 108, 176, 1400, 3894
Mox20I TGGCCA 2 cut(s) 276, 1733
Mph1103I ATGCAT 3 cut(s) 1171, 1361, 3174
MroI TCCGGA 1 cut(s) 976
MroNI GCCGGC 1 cut(s) 3851
MroXI GAANNNNTTC 3 cut(s) 534, 3534, 3921
MscI TGGCCA 2 cut(s) 276, 1733
MslI CAYNNNNRTG 6 cut(s) 1401, 2463, 3318, 3429, 3627, 4058
Msp20I TGGCCA 2 cut(s) 276, 1733
MspA1I CMGCKG 5 cut(s) 65, 272, 1456, 2243, 3968
MspCI CTTAAG 1 cut(s) 2587
MspI CCGG 6 cut(s) 31, 741, 977, 1127, 3852, 4148
MunI CAATTG 6 cut(s) 350, 1625, 2952, 3089, 3662, 3792
Mva1269I GAATGC 5 cut(s) 1607, 1785, 1970, 3745, 3775
MvnI CGCG 3 cut(s) 1146, 2260, 3690
NaeI GCCGGC 1 cut(s) 3853
NarI GGCGCC 1 cut(s) 3606
NciI CCSGG 1 cut(s) 4148
NcoI CCATGG 2 cut(s) 1614, 2427
NdeI CATATG 2 cut(s) 1470, 3378
NgoMIV GCCGGC 1 cut(s) 3851
NmuCI GTSAC 7 cut(s) 173, 290, 618, 845, 1300, 2158, 2677
NsbI TGCGCA 1 cut(s) 1594
NsiI ATGCAT 3 cut(s) 1171, 1361, 3174
NspI RCATGY 5 cut(s) 414, 426, 1402, 2903, 3594
NspV TTCGAA 1 cut(s) 1487
PaeR7I CTCGAG 1 cut(s) 3679
PagI TCATGA 2 cut(s) 2605, 4268
PasI CCCWGGG 2 cut(s) 1709, 4082
PceI AGGCCT 2 cut(s) 961, 3137
PciI ACATGT 1 cut(s) 1398
PciSI GCTCTTC 1 cut(s) 3501
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 5 cut(s) 1607, 1785, 1970, 3745, 3775
PdiI GCCGGC 1 cut(s) 3853
PdmI GAANNNNTTC 3 cut(s) 534, 3534, 3921
PfeI GAWTC 8 cut(s) 209, 251, 365, 1478, 2703, 2827, 2912, 3013
PflFI GACNNNGTC 1 cut(s) 686
PleI GAGTC 7 cut(s) 110, 130, 677, 1802, 3671, 3686, 4039
PluTI GGCGCC 1 cut(s) 3609
PpsI GAGTC 7 cut(s) 110, 130, 677, 1802, 3671, 3686, 4039
PpuMI RGGWCCY 2 cut(s) 700, 2104
PscI ACATGT 1 cut(s) 1398
PshAI GACNNNNGTC 1 cut(s) 3379
PshBI ATTAAT 2 cut(s) 2472, 3450
PsiI TTATAA 2 cut(s) 1719, 3272
Psp124BI GAGCTC 1 cut(s) 1291
Psp5II RGGWCCY 2 cut(s) 700, 2104
PspEI GGTNACC 4 cut(s) 290, 845, 2158, 2180
PspOMI GGGCCC 1 cut(s) 4077
PspPPI RGGWCCY 2 cut(s) 700, 2104
PspXI VCTCGAGB 1 cut(s) 3679
PsrI GAACNNNNNNTAC 2 cut(s) 3354, 3386
PstI CTGCAG 1 cut(s) 3153
PstNI CAGNNNCTG 2 cut(s) 3044, 3968
PsuI RGATCY 6 cut(s) 557, 1444, 1928, 2401, 2530, 3472
PsyI GACNNNGTC 1 cut(s) 686
PvuII CAGCTG 5 cut(s) 65, 272, 1456, 2243, 3968
RsaI GTAC 3 cut(s) 583, 1295, 3467
RsaNI GTAC 3 cut(s) 582, 1294, 3466
RseI CAYNNNNRTG 6 cut(s) 1401, 2463, 3318, 3429, 3627, 4058
SacI GAGCTC 1 cut(s) 1291
SalI GTCGAC 1 cut(s) 1829
SapI GCTCTTC 1 cut(s) 3501
SchI GAGTC 7 cut(s) 110, 131, 677, 1802, 3671, 3686, 4039
SduI GDGCHC 4 cut(s) 571, 1291, 1502, 4081
SfcI CTRYAG 5 cut(s) 829, 1533, 3024, 3149, 3846
SfoI GGCGCC 1 cut(s) 3607
Sfr274I CTCGAG 1 cut(s) 3679
SfuI TTCGAA 1 cut(s) 1487
SinI GGWCC 4 cut(s) 700, 2104, 2505, 3295
SlaI CTCGAG 1 cut(s) 3679
SmiMI CAYNNNNRTG 6 cut(s) 1401, 2463, 3318, 3429, 3627, 4058
SmlI CTYRAG 6 cut(s) 1448, 2071, 2576, 2587, 3679, 3777
SmoI CTYRAG 6 cut(s) 1448, 2071, 2576, 2587, 3679, 3777
SseBI AGGCCT 2 cut(s) 961, 3137
SsiI CCGC 7 cut(s) 231, 1054, 1250, 1279, 1442, 2258, 3855
SspDI GGCGCC 1 cut(s) 3605
SspMI CTAG 8 cut(s) 171, 186, 341, 963, 1115, 1155, 2700, 2892
SstI GAGCTC 1 cut(s) 1291
StuI AGGCCT 2 cut(s) 961, 3137
StyI CCWWGG 4 cut(s) 1614, 1872, 2427, 3060
TaaI ACNGT 6 cut(s) 157, 1918, 2642, 2748, 3124, 4016
TaiI ACGT 2 cut(s) 2379, 4009
TatI WGTACW 1 cut(s) 1293
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 8 cut(s) 209, 251, 365, 1478, 2703, 2827, 2912, 3013
TscAI CASTG 9 cut(s) 1507, 1921, 2050, 2170, 2299, 2686, 3313, 4021, 4047
TseFI GTSAC 7 cut(s) 173, 290, 618, 845, 1300, 2158, 2677
Tsp45I GTSAC 7 cut(s) 173, 290, 618, 845, 1300, 2158, 2677
TspGWI ACGGA 5 cut(s) 676, 925, 1910, 2612, 2912
TspRI CASTG 9 cut(s) 1507, 1921, 2050, 2170, 2299, 2686, 3313, 4021, 4047
Tth111I GACNNNGTC 1 cut(s) 686
Vha464I CTTAAG 1 cut(s) 2587
VpaK11BI GGWCC 4 cut(s) 700, 2104, 2505, 3295
VspI ATTAAT 2 cut(s) 2472, 3450
XagI CCTNNNNNAGG 1 cut(s) 285
XbaI TCTAGA 1 cut(s) 2699
XceI RCATGY 5 cut(s) 414, 426, 1402, 2903, 3594
XcmI CCANNNNNNNNNTGG 4 cut(s) 1107, 1489, 3067, 4139
XhoI CTCGAG 1 cut(s) 3679
XmiI GTMKAC 2 cut(s) 1532, 1830
XmnI GAANNNNTTC 3 cut(s) 534, 3534, 3921
XspI CTAG 8 cut(s) 171, 186, 341, 963, 1115, 1155, 2700, 2892
Zsp2I ATGCAT 3 cut(s) 1171, 1361, 3174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.