pycom02g26510

mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005851
Physical Location & Seq
Forward (+)
24273357 .. 24277649
4293 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26510.1

Sequence Viewer

Length: 1584 bp
ATGCAGTCTAGAACACCCAAAAAAGAACAAACAGGACAGAAATCTCCAGGAAAATCATCCAAGGGTCAAGGAAAATTGAAGGATGGAGCATCTGCTAGAAAGAGTCGATCCTCATTTATGCTTGCTAGCTCAGAAACTCTATGGTCCGACATTCAACAATTCGCAAAACTCAAATATCAGTTTGAGTTGCCGGAGGATGCTAGGATGCATGTAAAGAAAGATTCTGTTATACGTAATCTTTGCCAAAAGATGGGCATTACAATTGCAGCTCGCAGATATGATCTTAACTCTGTAGCACCCTTCCAAATGTCAGATATCTTAAATCTTCAACCTGTAGTGAAACACTCAGTTCCTGTGTGTTCAGAAGCTAAGGATCTTGTGGAAACAGGAAAGATCCAACTCGCCGAGGGAATGTTAAGTGAAGCCTACACATTATTTACTGAGGCATTTTCGATACTTCAGCAGGTTACTGGTCCAATGCATCGGGAGGTTGCTAACTGCTGTCGGTATCTTGCCATGGTTCTGTATCATGCAGGAGACATGGCTGGAGCCATAATGCAACAGCATAAGGAACTCATTATAAATGAACGCTGCTTGGGCCTAGACCACCCTGACACTGCTCACAGCTATGGAAATATGGCTCTTTTCTACCATGGACTCAACCAGACAGAACTCGCACTACGGCACATGTCGAGGGCATTGCTCCTGCTAAGTTTGTCATCTGGACCAGATCACCCTGATGTTGCTGCAACTTTCATTAATGTCGCTATGATGTATCAAGATCTTGGGAAGATGGATACTGCTCTTCGATATTTGCAAGAGGCCCTGAAAAAAAATGAAAGGCTTCTGGGGGAGGAACACATCCAGACTGCTGTATGCTATCATGCACTTGCTATTGCATTTAATTGTATGGGTGCCTTCAAGCTCTCTCACCAGCATGAAAAGAAAACCTATGACATACTTGTCAAACAATTGGGCGAGGAGGATTCTAGAACACGTGACTCTCAAAACTGGATGAAGACATTTAAGATGCGTGAGCTACAGATGAATGCACAAAAGCAAAAAGGTCAAGCTTTGAGTGCTGCTTCAGCTCAAAAGGCAATTGACATCTTGAAGGCTCATCCTGACTTGATGCAGGCATTCCAGTCTGCTGCCAAATCCGGTGGGTCTGGGAGCTCTAACCCCTCCGTCAACAAATCTCTTAATGCTGCAATAATTGGTGATACTCTTCCAAGAGGTAGGGGAGTTGATGAGAGGGCTGCTCGTGCAGCAGCTGAAGTCAGGAGGAAGGCAGCAGCAAGGGGCCTCTTGGTACGCCCACACGGTGTTCCAGTCCAAGCCTTGCCTCCACTTACTCAGTTCCTCGATATCATCAACTCAGGTGCAACCCCACCAGTTGCTGCAGAAAATGGAGAAACTGATGGAGCGAAGGAAGCCAATAGTCATCCTGCAAATGGCTCAGCTGATGTGAAACAAGAGCAAGCAACAGCGGAGCAGGAAGCTCAGCCTCCCGTTGGATTGGGCAAAGGCTTAGCAACACTGGACGGCAAGAAACAGAAATCGAAAAGCAAAGCCACATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N DUF727 TPR_10 TPR_12 eIF3_p135

Protein Analysis

528

Amino Acids

57.5

Weight (kDa)

9.18

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_p135 PF12807 27 - 94 1.9e-12 Translation initiation factor eIF3 subunit 135
TPR_NPHP3 PF24885 102 - 234 3.7e-06 Nephrocystin-3 TPR domain
TPR_12 PF13424 171 - 234 3.6e-09 Tetratricopeptide repeat
TPR_NPHP3 PF24885 208 - 348 2.3e-13 Nephrocystin-3 TPR domain
TPR_12 PF13424 210 - 277 4.9e-12 Tetratricopeptide repeat
TPR_12 PF13424 247 - 320 1.7e-14 Tetratricopeptide repeat
TPR_10 PF13374 248 - 288 3.2e-09 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 581
AasI GACNNNNNNGTC 1 cut(s) 962
Acc36I ACCTGC 1 cut(s) 454
AccB1I GGYRCC 1 cut(s) 914
AccB7I CCANNNNNTGG 1 cut(s) 250
AciI CCGC 1 cut(s) 1490
AclWI GGATC 3 cut(s) 102, 381, 388
AcuI CTGAAG 3 cut(s) 443, 1071, 1296
AcvI CACGTG 1 cut(s) 998
AdeI CACNNNGTG 1 cut(s) 1325
AfaI GTAC 1 cut(s) 1314
AfiI CCNNNNNNNGG 3 cut(s) 250, 1454, 1514
AflIII ACRYGT 2 cut(s) 687, 995
AgsI TTSAA 5 cut(s) 79, 155, 329, 922, 1114
AjnI CCWGG 1 cut(s) 46
Alw21I GWGCWC 1 cut(s) 1178
Alw26I GTCTC 1 cut(s) 531
AlwI GGATC 3 cut(s) 102, 381, 388
AlwNI CAGNNNCTG 3 cut(s) 353, 1274, 1400
AoxI GGCC 3 cut(s) 598, 822, 1303
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 843
AspS9I GGNCC 6 cut(s) 144, 473, 598, 725, 823, 1303
AsuHPI GGTGA 3 cut(s) 725, 923, 1232
AsuNHI GCTAGC 1 cut(s) 125
AvaII GGWCC 3 cut(s) 144, 473, 725
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 1 cut(s) 1178
BauI CACGAG 1 cut(s) 1263
BbrPI CACGTG 1 cut(s) 998
BbsI GAAGAC 1 cut(s) 1025
Bbv12I GWGCWC 1 cut(s) 1178
BccI CCATC 4 cut(s) 77, 244, 787, 1415
BceAI ACGGC 2 cut(s) 698, 1561
BcgI CGANNNNNNTGC 2 cut(s) 682, 716
BciT130I CCWGG 1 cut(s) 48
BciVI GTATCC 1 cut(s) 790
BcoDI GTCTC 1 cut(s) 531
BfaI CTAG 6 cut(s) 9, 96, 126, 201, 602, 990
BfmI CTRYAG 4 cut(s) 291, 333, 1040, 1401
BfuAI ACCTGC 1 cut(s) 454
BfuI GTATCC 1 cut(s) 790
BglII AGATCT 1 cut(s) 781
BlpI GCTNAGC 3 cut(s) 1459, 1503, 1531
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 3 cut(s) 144, 473, 725
BmgT120I GGNCC 6 cut(s) 144, 473, 598, 725, 823, 1303
BmiI GGNNCC 3 cut(s) 550, 916, 1304
BmrFI CCNGG 1 cut(s) 48
BmsI GCATC 6 cut(s) 98, 187, 195, 490, 1020, 1122
BmtI GCTAGC 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 1025
BplI GAGNNNNNCTC 2 cut(s) 1246, 1278
BpmI CTGGAG 2 cut(s) 30, 567
Bpu10I CCTNAGC 1 cut(s) 369
Bpu1102I GCTNAGC 3 cut(s) 1459, 1503, 1531
BsaAI YACGTR 2 cut(s) 233, 998
BsaJI CCNNGG 4 cut(s) 60, 405, 516, 652
BsaWI WCCGGW 1 cut(s) 1160
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 1454, 1514
Bse1I ACTGG 6 cut(s) 475, 1016, 1144, 1331, 1394, 1545
Bse3DI GCAATG 1 cut(s) 698
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 4 cut(s) 60, 405, 516, 652
BseGI GGATG 8 cut(s) 56, 88, 202, 210, 861, 1020, 1120, 1444
BseLI CCNNNNNNNGG 3 cut(s) 250, 1454, 1514
BseMI GCAATG 1 cut(s) 698
BseMII CTCAG 7 cut(s) 144, 360, 432, 1370, 1392, 1473, 1517
BseNI ACTGG 6 cut(s) 475, 1016, 1144, 1331, 1394, 1545
BseRI GAGGAG 1 cut(s) 995
BsgI GTGCAG 1 cut(s) 1287
BshFI GGCC 3 cut(s) 600, 824, 1305
BshNI GGYRCC 1 cut(s) 914
BsiHKAI GWGCWC 1 cut(s) 1178
BsiSI CCGG 2 cut(s) 191, 1161
BslI CCNNNNNNNGG 3 cut(s) 250, 1454, 1514
BsmAI GTCTC 1 cut(s) 531
BsmI GAATGC 2 cut(s) 1054, 1139
BsnI GGCC 3 cut(s) 600, 824, 1305
Bsp1286I GDGCHC 1 cut(s) 1178
Bsp143I GATC 6 cut(s) 107, 280, 373, 393, 730, 781
Bsp1720I GCTNAGC 3 cut(s) 1459, 1503, 1531
Bsp19I CCATGG 2 cut(s) 516, 652
BspACI CCGC 1 cut(s) 1490
BspANI GGCC 3 cut(s) 600, 824, 1305
BspCNI CTCAG 7 cut(s) 143, 359, 433, 1369, 1391, 1472, 1516
BspLI GGNNCC 3 cut(s) 550, 916, 1304
BspMAI CTGCAG 1 cut(s) 1405
BspMI ACCTGC 1 cut(s) 454
BspOI GCTAGC 1 cut(s) 129
BspPI GGATC 3 cut(s) 102, 381, 388
BspQI GCTCTTC 1 cut(s) 810
BspT107I GGYRCC 1 cut(s) 914
BsrDI GCAATG 1 cut(s) 698
BsrI ACTGG 6 cut(s) 475, 1016, 1144, 1331, 1394, 1545
BssECI CCNNGG 4 cut(s) 60, 405, 516, 652
BssMI GATC 6 cut(s) 107, 280, 373, 393, 730, 781
BssSI CACGAG 1 cut(s) 1263
BssT1I CCWWGG 3 cut(s) 60, 516, 652
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 1 cut(s) 1325
Bst6I CTCTTC 2 cut(s) 810, 1233
BstBAI YACGTR 2 cut(s) 233, 998
BstC8I GCNNGC 5 cut(s) 123, 127, 271, 1137, 1482
BstDSI CCRYGG 2 cut(s) 516, 652
BstF5I GGATG 8 cut(s) 56, 88, 202, 210, 861, 1020, 1120, 1444
BstKTI GATC 6 cut(s) 110, 283, 376, 396, 733, 784
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 6 cut(s) 107, 280, 373, 393, 730, 781
BstMWI GCNNNNNNNGC 7 cut(s) 597, 1079, 1088, 1097, 1265, 1268, 1433
BstNI CCWGG 1 cut(s) 48
BstNSI RCATGY 2 cut(s) 212, 691
BstSCI CCNGG 1 cut(s) 46
BstSFI CTRYAG 4 cut(s) 291, 333, 1040, 1401
BstSNI TACGTA 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 1025
BstX2I RGATCY 3 cut(s) 373, 393, 781
BstYI RGATCY 3 cut(s) 373, 393, 781
BsuI GTATCC 1 cut(s) 790
BsuRI GGCC 3 cut(s) 600, 824, 1305
BtgI CCRYGG 2 cut(s) 516, 652
BtsCI GGATG 8 cut(s) 56, 88, 202, 210, 861, 1020, 1120, 1444
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 615, 1538
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 5 cut(s) 123, 127, 271, 1137, 1482
CaiI CAGNNNCTG 3 cut(s) 353, 1274, 1400
Cfr13I GGNCC 6 cut(s) 144, 473, 598, 725, 823, 1303
Csp6I GTAC 1 cut(s) 1313
CspCI CAANNNNNGTGG 2 cut(s) 1144, 1179
CviAII CATG 8 cut(s) 209, 517, 530, 541, 653, 688, 884, 938
CviQI GTAC 1 cut(s) 1313
DpnI GATC 6 cut(s) 109, 282, 375, 395, 732, 783
DpnII GATC 6 cut(s) 107, 280, 373, 393, 730, 781
DraIII CACNNNGTG 1 cut(s) 1325
DrdI GACNNNNNNGTC 1 cut(s) 962
DseDI GACNNNNNNGTC 1 cut(s) 962
Eam1104I CTCTTC 2 cut(s) 810, 1233
EarI CTCTTC 2 cut(s) 810, 1233
Ecl136II GAGCTC 1 cut(s) 1176
Eco105I TACGTA 1 cut(s) 233
Eco130I CCWWGG 3 cut(s) 60, 516, 652
Eco24I GRGCYC 1 cut(s) 1178
Eco32I GATATC 2 cut(s) 316, 1369
Eco47I GGWCC 3 cut(s) 144, 473, 725
Eco53kI GAGCTC 1 cut(s) 1176
Eco57I CTGAAG 3 cut(s) 443, 1071, 1296
Eco72I CACGTG 1 cut(s) 998
EcoICRI GAGCTC 1 cut(s) 1176
EcoO109I RGGNCCY 2 cut(s) 823, 1303
EcoRII CCWGG 1 cut(s) 46
EcoRV GATATC 2 cut(s) 316, 1369
EcoT14I CCWWGG 3 cut(s) 60, 516, 652
EcoT22I ATGCAT 2 cut(s) 210, 483
EcoT38I GRGCYC 1 cut(s) 1178
ErhI CCWWGG 3 cut(s) 60, 516, 652
FaeI CATG 8 cut(s) 212, 520, 533, 544, 656, 691, 887, 941
FatI CATG 8 cut(s) 208, 516, 529, 540, 652, 687, 883, 937
FokI GGATG 8 cut(s) 43, 95, 209, 217, 848, 1027, 1107, 1431
FriOI GRGCYC 1 cut(s) 1178
FspBI CTAG 6 cut(s) 9, 96, 126, 201, 602, 990
GsuI CTGGAG 2 cut(s) 30, 567
HaeIII GGCC 3 cut(s) 600, 824, 1305
HapII CCGG 2 cut(s) 191, 1161
Hin1II CATG 8 cut(s) 212, 520, 533, 544, 656, 691, 887, 941
HincII GTYRAC 1 cut(s) 1192
HindII GTYRAC 1 cut(s) 1192
HindIII AAGCTT 1 cut(s) 1071
HinfI GANTC 5 cut(s) 103, 221, 657, 986, 1001
HpaII CCGG 2 cut(s) 191, 1161
HphI GGTGA 3 cut(s) 725, 923, 1232
Hpy166II GTNNAC 1 cut(s) 1192
Hpy188I TCNGA 4 cut(s) 133, 148, 313, 364
Hpy8I GTNNAC 1 cut(s) 1192
HpyAV CCTTC 6 cut(s) 73, 310, 928, 1108, 1282, 1423
HpyCH4III ACNGT 1 cut(s) 1325
HpyCH4IV ACGT 2 cut(s) 232, 997
HpyF10VI GCNNNNNNNGC 7 cut(s) 597, 1079, 1088, 1097, 1265, 1268, 1433
HpySE526I ACGT 2 cut(s) 232, 997
Hsp92II CATG 8 cut(s) 212, 520, 533, 544, 656, 691, 887, 941
Kzo9I GATC 6 cut(s) 107, 280, 373, 393, 730, 781
LguI GCTCTTC 1 cut(s) 810
LmnI GCTCC 6 cut(s) 86, 548, 708, 1173, 1424, 1492
LweI GCATC 6 cut(s) 98, 187, 195, 490, 1020, 1122
MaeI CTAG 6 cut(s) 9, 96, 126, 201, 602, 990
MaeII ACGT 2 cut(s) 232, 997
MaeIII GTNAC 2 cut(s) 466, 998
MalI GATC 6 cut(s) 109, 282, 375, 395, 732, 783
MboI GATC 6 cut(s) 107, 280, 373, 393, 730, 781
MboII GAAGA 5 cut(s) 317, 797, 802, 1030, 1220
MfeI CAATTG 3 cut(s) 261, 971, 1101
MflI RGATCY 3 cut(s) 373, 393, 781
MhlI GDGCHC 1 cut(s) 1178
MluCI AATT 7 cut(s) 74, 158, 261, 904, 971, 1101, 1215
MlyI GAGTC 3 cut(s) 112, 651, 995
MmeI TCCRAC 3 cut(s) 171, 421, 1495
Mph1103I ATGCAT 2 cut(s) 210, 483
MroXI GAANNNNTTC 1 cut(s) 843
MseI TTAA 7 cut(s) 285, 320, 416, 759, 903, 1026, 1203
MslI CAYNNNNRTG 3 cut(s) 627, 738, 936
MspA1I CMGCKG 3 cut(s) 1274, 1463, 1490
MspI CCGG 2 cut(s) 191, 1161
MspR9I CCNGG 1 cut(s) 48
MunI CAATTG 3 cut(s) 261, 971, 1101
Mva1269I GAATGC 2 cut(s) 1054, 1139
MvaI CCWGG 1 cut(s) 48
MwoI GCNNNNNNNGC 7 cut(s) 597, 1079, 1088, 1097, 1265, 1268, 1433
NcoI CCATGG 2 cut(s) 516, 652
NdeII GATC 6 cut(s) 107, 280, 373, 393, 730, 781
NheI GCTAGC 1 cut(s) 125
NlaIII CATG 8 cut(s) 212, 520, 533, 544, 656, 691, 887, 941
NlaIV GGNNCC 3 cut(s) 550, 916, 1304
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 1 cut(s) 998
NsiI ATGCAT 2 cut(s) 210, 483
NspI RCATGY 2 cut(s) 212, 691
PciI ACATGT 1 cut(s) 687
PciSI GCTCTTC 1 cut(s) 810
PctI GAATGC 2 cut(s) 1054, 1139
PdmI GAANNNNTTC 1 cut(s) 843
PfeI GAWTC 2 cut(s) 221, 986
PflMI CCANNNNNTGG 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 46
PleI GAGTC 3 cut(s) 111, 651, 995
PmaCI CACGTG 1 cut(s) 998
PmlI CACGTG 1 cut(s) 998
PpsI GAGTC 3 cut(s) 111, 651, 995
Ppu21I YACGTR 2 cut(s) 233, 998
PscI ACATGT 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 759
PsiI TTATAA 1 cut(s) 581
Psp124BI GAGCTC 1 cut(s) 1178
Psp6I CCWGG 1 cut(s) 46
PspCI CACGTG 1 cut(s) 998
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 3 cut(s) 550, 916, 1304
PspPI GGNCC 6 cut(s) 144, 473, 598, 725, 823, 1303
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PstI CTGCAG 1 cut(s) 1405
PstNI CAGNNNCTG 3 cut(s) 353, 1274, 1400
PsuI RGATCY 3 cut(s) 373, 393, 781
PvuII CAGCTG 2 cut(s) 1274, 1463
RsaI GTAC 1 cut(s) 1314
RsaNI GTAC 1 cut(s) 1313
RseI CAYNNNNRTG 3 cut(s) 627, 738, 936
SacI GAGCTC 1 cut(s) 1178
SapI GCTCTTC 1 cut(s) 810
SaqAI TTAA 7 cut(s) 285, 320, 416, 759, 903, 1026, 1203
Sau3AI GATC 6 cut(s) 107, 280, 373, 393, 730, 781
Sau96I GGNCC 6 cut(s) 144, 473, 598, 725, 823, 1303
SchI GAGTC 3 cut(s) 112, 651, 995
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 1 cut(s) 1178
SfaNI GCATC 6 cut(s) 98, 187, 195, 490, 1020, 1122
SfcI CTRYAG 4 cut(s) 291, 333, 1040, 1401
SinI GGWCC 3 cut(s) 144, 473, 725
SmiMI CAYNNNNRTG 3 cut(s) 627, 738, 936
SnaBI TACGTA 1 cut(s) 233
Sse9I AATT 7 cut(s) 74, 158, 261, 904, 971, 1101, 1215
SsiI CCGC 1 cut(s) 1490
SspMI CTAG 6 cut(s) 9, 96, 126, 201, 602, 990
SstI GAGCTC 1 cut(s) 1178
StyD4I CCNGG 1 cut(s) 46
StyI CCWWGG 3 cut(s) 60, 516, 652
TaaI ACNGT 1 cut(s) 1325
TaiI ACGT 2 cut(s) 235, 1000
TaqI TCGA 6 cut(s) 106, 452, 692, 808, 1365, 1562
TasI AATT 7 cut(s) 74, 158, 261, 904, 971, 1101, 1215
TfiI GAWTC 2 cut(s) 221, 986
Tru1I TTAA 7 cut(s) 285, 320, 416, 759, 903, 1026, 1203
Tru9I TTAA 7 cut(s) 285, 320, 416, 759, 903, 1026, 1203
TscAI CASTG 2 cut(s) 622, 1545
TseFI GTSAC 1 cut(s) 998
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 6 cut(s) 600, 745, 852, 954, 1031, 1061
TspGWI ACGGA 1 cut(s) 1177
TspRI CASTG 2 cut(s) 622, 1545
Van91I CCANNNNNTGG 1 cut(s) 250
VpaK11BI GGWCC 3 cut(s) 144, 473, 725
VspI ATTAAT 1 cut(s) 759
XbaI TCTAGA 2 cut(s) 8, 989
XceI RCATGY 2 cut(s) 212, 691
XmnI GAANNNNTTC 1 cut(s) 843
XspI CTAG 6 cut(s) 9, 96, 126, 201, 602, 990
Zsp2I ATGCAT 2 cut(s) 210, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.