MD02G1316400.v1.1

mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005851
Physical Location & Seq
Forward (+)
37082315 .. 37096087
13773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1316400.v1.1.491

Sequence Viewer

Length: 4221 bp
ATGGCTGGAAAGTCGAATAAAGGGAGGAACCGTAGAGGTGCGAGCAATGCTGTGGTCCCATCTGATGCTCCTGTGAAAGATAACTCAAGCGCTTCAGAGCCCATTAAAGCCGAAGACAATGGTGTTCCAGCTGTTGAAGAATCAACTGCTAGTCTGGAGGTAAAGGAGTCTGAAACAGAAAATTCAACCAGCCAAACTAAGCAAGGTGACCTACATCTGTATCCAGTTTCTGTCAAGACACAAAGTGGCGAGAAGCTTGAGCTACAATTGAACCCAGGAGACTCTGTTATGGATATAAGACAATTTCTGCTTGATGCACCGGAGACATGTTTCTTCACATGTTATGACTTATTGCTACACACAAAGGATGGCTCCACTCATCATTTGGAAGACTTTAATGAAATTTCTGAAGTTGCTGATATTACCATTGGTGGCTGTTCCTTGGAGATGGTTCCTGCTTTATATGATGATAGATCTATACGGGCTCATGTTCATCGTACGAGAGAATTGCTTTCCCTTTCTACACTTCATGCATCACTATCGACATCACTTGCATTACAGTATGAGACTGCACAAAACAAAGTGGCGAGCCCTGGAGATACGGTGAAAACTGAGGTCCCAGAGCTTGATGGTTTGGGTTTTATGGAGGACATTGCTGGTTCATTGAGCAATTTGCTATCTTCACCCTCAAAAGAGATCAAATGCGTGGAGAGTATTGTGTTTTCATCCTTCAATCCTCCACCAAGCTATAGAAGGCTTGTTGGGGATTTGATTTATTTGGATGTAGTAACATTGGAGGGTAATAAACACTGTATTACTGGGACTACAAAAATGTTCTATGTCAATTCGAGCACGGGGAACTCCCTTGACCCCAAGCTGAGTAAAAGTAATTCGGAAGCAACCACACTTGTTGGACTTTTGCAAAGGATTAGCTCTAAGTTCAAAAAAGCTTTTCATGAAATTTTGGAGCGGAGGGCATCTGCACATCCTTTTGAAAATGTGCAGTCCTTGTTGCCACCAAATTCATGGCTAGGGTTATATCCAGTTCCAGATCACAAGCGTGATGCAGCTAGAGCTGAGGATTCTCTTACTCTTTCATATGGGAGTGAGCTAATCGGCATGCAAAGGGATTGGAATGAGGAGTTGCAATCTTGTAGGGAATTTCCGCATACAACTCCTCAGGAAAGAATTTTGCGGGATAGGGCTCTGTACAAAGTGACTTCAGACTTTGTTGATGCAGCTATTAGTGGTGCTATTGGAGTTATTAGTAGATGCATACCTCCTATAAACCCAACTGATCCAGAGTGCTTCCACATGTATGTTCACAATAATATATTCTTCAGTTTTGCTGTTGATGCGGACCTTGAGCAACTGTCTAAGAAACATGCATCATATTCTAGTCCAAAGATTGGGGGCTCTGGTTTTTTACACAGTTCTGAGAAGGCTCCTGACAGTTTGTTGCATGGAGAGAGTGCAATTCCCAATGGAGAAAAATGCAAAGGATCAAGTACAGTTGATGATGCCACTGAATCCTCTACTGATGTTTCTGCCGAGACTCAATTGGGTGAAACTGAGCAGGCTACTTATGCTTCTGCAAATAATGATTTGAAAGGCACTAAGGCGTACCAGGAAGCTGATGTCTCTGGACTTTATAATCTTGCTATGGCCATAATCGATTACAGGGGGCATAGAGTAGTAGCTCAGAGTGTTTTACCGGGAATTCTTCAAGGGGATAAATCAGACTCTCTTCTGTACGGTTCTGTTGATAATGGCAAGAAAATATGCTGGAATGAAGAGTTTCATTCCAAGGTAGTGGAAGCAGCTAAGCGCCTTCATCTGAAAGAGCATACAGTCCTGGATGGATCTGGAAATGTTTTCAGATTGGCTGCACCGGTGGAATGCAAGGGTATTGTTGGTAGTGATGACAGGCATTATCTCCTCGACTTAATGAGAGTGACTCCTCGTGATGCAAATTTCACTGGATCTGGGTCCAGATTTTGTATCTTGAGGCCAGAACTAATTACTGCCTATTGCCAGGCCCAAGCTGCTGAGAAACCAAAGTCTAAGTCTAAAGATGGAGAAGGTATTATCACCACTGATTCTTCAGTCATCACTGATGCTAAGCAGGACATAACGGAAGAAGGGAAGGCCACAGATGCACAAGAAAGTGCGTCTCCACCTCCACACACTGATAGCTCTGATCCATGTGAAGAAATTCTTTTTAACCCTAATGTCTTTACCGAGTTTAAGTTGGCTGGAAATGAGGAGGAGATGGCAGAAGATGAAGGAAATGTGAGGAAAGCCAGTTTGTATCTTACAGATGTTGTGCTTCCAAAATTTATACAAGACCTCTGCACGCTTGAAGTATCACCCATGGATGGGCAGACACTAACTGAAGCACTCCATGCTCATGGAATTAATGTTCGCTATATTGGAAAAGTGGCTGATGGGACCAGACATTTACCTCATCTTTGGGATCTTTGCTCCAATGAGATAATGGTTAGATCTGCCAAGCACATTCTCAAGGATGCCTTGAGAGAAACAGACGATCATGATATTGGGCCCGCAATCACCCATTTCTTCAACTGTTTCTTTGGAAGCTGTCAAGCAGTTGGCTCAAAAGTCACTGCTAATAATATGCAGTCTAGAACCCCCAAAAAAGAACAAACAGGACAGAAATCTCCAGGAAAATCGTCCAAGGGACAAGGAAAATTGAAGGATCGAGCATCTGCTAGAAAGAGTCGATCCTCATTTATGCTTGCTAGCTCAGAAACTCTATGGTCCGACATTCAAGAATTCGCAAAACTCAAATATCAGTTTGAGTTGCCGGAGGATGCTAGGATGCGTGTAAAGAAAGATTCTGTTATACGTAATCTTTGCCAAAAGATGGGCATTACAATTGCAGCTCGCAGATATGATCTTAACTCCGTAGCACCCTTCCAAATGTCAGATATCTTAAATCTTCAACCTGTAGTAAAACACTCAGTTCCTGTGTGTTCAGAAGCTAAGGATCTTGTGGAAACAGGAAAGATCCAACTGGCCGAGGGAATGTTAAGTGAAGCCTACACATTATTTACTGAGGCATTTTCGATACTTCAGCAGGTTACTGGTCCAATGCATCGGGAGGTTGCTAACTGCTGTCGGTATCTTGCCATGGTTCTGTATCATGCAGGAGACATGGCTGGAGCCATAATGCAACAGCATAAGGAACTAATTATAAATGAACGCTGCCTGGGCCTAGACCACCCTGACACTGCTCACAGCTATGGAAATATGGCTCTTTTCTATCATGGACTCAACCAGACAGAACTCGCACTACGGCACATGTCGAGGGCATTGCTCCTGCTAAGTTTGTCATCTGGACCTGATCACCCTGATGTTGCTGCAACTTTCATTAATGTCGCTATGATGTACCAAGATCTTGGGAAGATGGACACTGCTCTTCGATATTTGCAAGAGGCTCTGAAAAAAAATGAAAGGCTTCTGGGGGAGGAACACATCCAGACTGCTGTATGCTATCATGCACTTGCTATTGCATGTAATTGTATGGGTGCCTTCAAGCTCTCTCACCAGCATGAGAAGAAAACCTATGACATACTTGTCAAACAATTGGGCGAGGAGGATTCTAGGACACGTGACTCTCAAAACTGGATGAAGACATTTAAGATGCGTGAGCTACAGATGAATGCACAAAAGCAAAAAGGTCAAGCTTTGAATGCTGCTTCAGCTCAAAAGGCAATTGACATCTTGAAGGCTCATCCTGACTTGATGCAGGCATTCCAATCTGCTGCCAAATCCGGTGGGTCTGGGAGCTCTAACCCCTCCGTCAACAAATCTCTTAATGCTGCAATAATTGGTGATACTCTTCCACGAGGTAGGGGAGTTGATGAGAGGGCTGCTCGTGCAGCAGCTGAAGTCAGGAGGAAGGCAGCAGCAAGGGGCCTCTTGGTACGCCCACACGGTGTTCCGGTCCAAGCCTTGCCTCCACTTACTCAGTTCCTCGATATCATCAACTCAGGTGCGACCCCACCAGTTGCTGCAGAAAATGGAGAAACTGATGGAGTGAAGGATGCCAATAGTCATCCTGAGAATGGCGCAGCTGATGTGAAACAAGAGCAAGCAACAGCGGAGCAGGAAGCTCAACCTCCCGTTGGATTGGGCAAAGGCTTAGCAACACTGGACAGCAAGAAACAGAAATCAAAAAGCAAAGCCGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N DUF727 TPR_10 TPR_12 eIF3_p135

Protein Analysis

1407

Amino Acids

153.65

Weight (kDa)

5.89

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 94 - 170 2e-14 Mitochondrial function, CLU-N-term
CLU PF13236 377 - 463 9.4e-26 Clustered mitochondria
CLU PF13236 514 - 659 4.2e-47 Clustered mitochondria
eIF3_p135 PF12807 789 - 973 4.3e-47 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 1050 - 1113 1.2e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 1085 - 1227 4.4e-12 Nephrocystin-3 TPR domain
TPR_12 PF13424 1089 - 1156 1.1e-11 Tetratricopeptide repeat
TPR_12 PF13424 1126 - 1199 3.7e-14 Tetratricopeptide repeat
TPR_10 PF13374 1127 - 1167 1e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1653, 3218
AasI GACNNNNNNGTC 1 cut(s) 3599
Acc36I ACCTGC 1 cut(s) 3091
AccB1I GGYRCC 1 cut(s) 3551
AccB7I CCANNNNNTGG 2 cut(s) 1409, 2887
AccBSI CCGCTC 1 cut(s) 970
AciI CCGC 7 cut(s) 970, 1166, 1195, 1358, 2565, 4127, 4212
AcoI YGGCCR 2 cut(s) 1665, 3039
AcuI CTGAAG 9 cut(s) 78, 429, 1206, 1324, 2088, 2415, 3080, 3708, 3933
AcvI CACGTG 1 cut(s) 3635
AdeI CACNNNGTG 2 cut(s) 245, 3962
AfaI GTAC 7 cut(s) 499, 1211, 1510, 1625, 1754, 3413, 3951
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 6 cut(s) 1409, 2378, 2379, 2887, 4091, 4151
AflIII ACRYGT 5 cut(s) 326, 338, 1314, 3324, 3632
AgeI ACCGGT 1 cut(s) 1891
AjnI CCWGG 7 cut(s) 274, 592, 1626, 1854, 2034, 2683, 3231
AleI CACNNNNGTG 1 cut(s) 1059
AloI GAACNNNNNNTCC 2 cut(s) 2406, 2438
Alw21I GWGCWC 2 cut(s) 854, 3815
Alw26I GTCTC 7 cut(s) 273, 317, 560, 1547, 1645, 2178, 3168
AlwNI CAGNNNCTG 4 cut(s) 230, 2990, 3911, 4037
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 8 cut(s) 1665, 2009, 2037, 2148, 2561, 3039, 3235, 3940
ApaI GGGCCC 1 cut(s) 2565
ArsI GACNNNNNNTTYG 2 cut(s) 2051, 2083
AseI ATTAAT 2 cut(s) 2418, 3396
AsiGI ACCGGT 1 cut(s) 1891
Asp700I GAANNNNTTC 3 cut(s) 1797, 2214, 3480
AspLEI GCGC 3 cut(s) 92, 1830, 4097
AsuC2I CCSGG 1 cut(s) 1716
AsuNHI GCTAGC 1 cut(s) 2762
AvaII GGWCC 9 cut(s) 55, 616, 1360, 1989, 2451, 2781, 3110, 3362, 3970
AxyI CCTNAGG 1 cut(s) 1179
BaeGI GKGCMC 1 cut(s) 2565
BaeI ACNNNNGTAYC 2 cut(s) 203, 236
BalI TGGCCA 1 cut(s) 1667
BanI GGYRCC 1 cut(s) 3551
BanII GRGCYC 7 cut(s) 102, 487, 593, 1207, 1418, 2565, 3815
BauI CACGAG 3 cut(s) 1962, 3870, 3900
BbrPI CACGTG 1 cut(s) 3635
BbsI GAAGAC 3 cut(s) 120, 396, 3662
Bbv12I GWGCWC 2 cut(s) 854, 3815
BbvCI CCTCAGC 1 cut(s) 1077
BceAI ACGGC 1 cut(s) 3335
BcgI CGANNNNNNTGC 6 cut(s) 490, 522, 524, 556, 3319, 3353
BciT130I CCWGG 7 cut(s) 276, 594, 1628, 1856, 2036, 2685, 3233
BciVI GTATCC 1 cut(s) 231
BclI TGATCA 1 cut(s) 3367
BcnI CCSGG 1 cut(s) 1716
BcoDI GTCTC 7 cut(s) 273, 317, 560, 1547, 1645, 2178, 3168
BfmI CTRYAG 4 cut(s) 748, 2970, 3677, 4038
BfoI RGCGCY 2 cut(s) 93, 1831
BfuAI ACCTGC 1 cut(s) 3091
BfuI GTATCC 1 cut(s) 231
BglII AGATCT 3 cut(s) 473, 2504, 3418
BlpI GCTNAGC 3 cut(s) 1824, 2121, 4168
Bme1390I CCNGG 8 cut(s) 276, 594, 1628, 1716, 1856, 2036, 2685, 3233
Bme18I GGWCC 9 cut(s) 55, 616, 1360, 1989, 2451, 2781, 3110, 3362, 3970
BmrFI CCNGG 8 cut(s) 276, 594, 1628, 1716, 1856, 2036, 2685, 3233
BmrI ACTGGG 1 cut(s) 828
BmtI GCTAGC 1 cut(s) 2766
BmuI ACTGGG 1 cut(s) 828
BpiI GAAGAC 3 cut(s) 120, 396, 3662
BplI GAGNNNNNCTC 4 cut(s) 1942, 1974, 3883, 3915
BpmI CTGGAG 4 cut(s) 176, 615, 2667, 3204
Bpu10I CCTNAGC 2 cut(s) 1077, 3006
Bpu1102I GCTNAGC 3 cut(s) 1824, 2121, 4168
BpuEI CTTGAG 6 cut(s) 70, 278, 1385, 2026, 2507, 2554
BpuMI CCSGG 1 cut(s) 1716
Bsa29I ATCGAT 1 cut(s) 1674
BsaAI YACGTR 2 cut(s) 2870, 3635
BsaJI CCNNGG 9 cut(s) 274, 441, 592, 1806, 2373, 2697, 3042, 3153, 3232
BsaWI WCCGGW 4 cut(s) 319, 1891, 3797, 3967
BsaXI ACNNNNNCTCC 6 cut(s) 16, 46, 270, 300, 588, 618
Bsc4I CCNNNNNNNGG 6 cut(s) 1409, 2378, 2379, 2887, 4091, 4151
Bse118I RCCGGY 1 cut(s) 1891
Bse21I CCTNAGG 1 cut(s) 1179
Bse3DI GCAATG 3 cut(s) 52, 651, 3335
BseBI CCWGG 7 cut(s) 276, 594, 1628, 1856, 2036, 2685, 3233
BseCI ATCGAT 1 cut(s) 1674
BseDI CCNNGG 9 cut(s) 274, 441, 592, 1806, 2373, 2697, 3042, 3153, 3232
BseLI CCNNNNNNNGG 6 cut(s) 1409, 2378, 2379, 2887, 4091, 4151
BseMI GCAATG 3 cut(s) 52, 651, 3335
BseRI GAGGAG 7 cut(s) 1154, 1167, 1928, 1950, 2279, 2282, 3632
BseSI GKGCMC 1 cut(s) 2565
BsgI GTGCAG 6 cut(s) 555, 966, 1022, 1872, 2338, 3924
BshFI GGCC 8 cut(s) 1667, 2011, 2039, 2150, 2563, 3041, 3237, 3942
BshNI GGYRCC 1 cut(s) 3551
BshTI ACCGGT 1 cut(s) 1891
BshVI ATCGAT 1 cut(s) 1674
BsiHKAI GWGCWC 2 cut(s) 854, 3815
BsiSI CCGG 6 cut(s) 320, 1715, 1892, 2828, 3798, 3968
BsiWI CGTACG 1 cut(s) 497
BslFI GGGAC 5 cut(s) 41, 602, 835, 2464, 2715
BslI CCNNNNNNNGG 6 cut(s) 1409, 2378, 2379, 2887, 4091, 4151
BsmAI GTCTC 7 cut(s) 273, 317, 560, 1547, 1645, 2178, 3168
BsmBI CGTCTC 1 cut(s) 2178
BsmFI GGGAC 5 cut(s) 41, 602, 835, 2464, 2715
BsmI GAATGC 4 cut(s) 1904, 3691, 3721, 3776
BsnI GGCC 8 cut(s) 1667, 2011, 2039, 2150, 2563, 3041, 3237, 3942
Bsp120I GGGCCC 1 cut(s) 2561
Bsp1286I GDGCHC 8 cut(s) 102, 487, 593, 854, 1207, 1418, 2565, 3815
Bsp1407I TGTACA 1 cut(s) 1209
Bsp1720I GCTNAGC 3 cut(s) 1824, 2121, 4168
Bsp19I CCATGG 2 cut(s) 2373, 3153
BspACI CCGC 7 cut(s) 970, 1166, 1195, 1358, 2565, 4127, 4212
BspANI GGCC 8 cut(s) 1667, 2011, 2039, 2150, 2563, 3041, 3237, 3942
BspDI ATCGAT 1 cut(s) 1674
BspHI TCATGA 2 cut(s) 955, 2551
BspMAI CTGCAG 1 cut(s) 4042
BspMI ACCTGC 1 cut(s) 3091
BspOI GCTAGC 1 cut(s) 2766
BspQI GCTCTTC 1 cut(s) 3447
BspT107I GGYRCC 1 cut(s) 3551
BsrBI CCGCTC 1 cut(s) 970
BsrDI GCAATG 3 cut(s) 52, 651, 3335
BsrFI RCCGGY 1 cut(s) 1891
BsrGI TGTACA 1 cut(s) 1209
BssAI RCCGGY 1 cut(s) 1891
BssECI CCNNGG 9 cut(s) 274, 441, 592, 1806, 2373, 2697, 3042, 3153, 3232
BssSI CACGAG 3 cut(s) 1962, 3870, 3900
BssT1I CCWWGG 5 cut(s) 441, 1806, 2373, 2697, 3153
Bst2BI CACGAG 3 cut(s) 1962, 3870, 3900
Bst2UI CCWGG 7 cut(s) 276, 594, 1628, 1856, 2036, 2685, 3233
Bst6I CTCTTC 4 cut(s) 1752, 1788, 3447, 3870
BstAPI GCANNNNNTGC 1 cut(s) 2405
BstAUI TGTACA 1 cut(s) 1209
BstBAI YACGTR 2 cut(s) 2870, 3635
BstDSI CCRYGG 2 cut(s) 2373, 3153
BstEII GGTNACC 1 cut(s) 206
BstH2I RGCGCY 2 cut(s) 93, 1831
BstHHI GCGC 3 cut(s) 92, 1830, 4097
BstMAI GTCTC 7 cut(s) 273, 317, 560, 1547, 1645, 2178, 3168
BstNI CCWGG 7 cut(s) 276, 594, 1628, 1856, 2036, 2685, 3233
BstNSI RCATGY 7 cut(s) 330, 342, 1123, 1318, 1388, 3328, 3540
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 8 cut(s) 274, 592, 1626, 1714, 1854, 2034, 2683, 3231
BstSFI CTRYAG 4 cut(s) 748, 2970, 3677, 4038
BstSLI GKGCMC 1 cut(s) 2565
BstSNI TACGTA 1 cut(s) 2870
BstV2I GAAGAC 3 cut(s) 120, 396, 3662
BstX2I RGATCY 8 cut(s) 473, 1862, 1982, 2476, 2504, 3010, 3030, 3418
BstXI CCANNNNNNTGG 4 cut(s) 1026, 1813, 2411, 3422
BstYI RGATCY 8 cut(s) 473, 1862, 1982, 2476, 2504, 3010, 3030, 3418
Bsu15I ATCGAT 1 cut(s) 1674
Bsu36I CCTNAGG 1 cut(s) 1179
BsuI GTATCC 1 cut(s) 231
BsuRI GGCC 8 cut(s) 1667, 2011, 2039, 2150, 2563, 3041, 3237, 3942
BsuTUI ATCGAT 1 cut(s) 1674
BtgI CCRYGG 2 cut(s) 2373, 3153
BtsI GCAGTG 3 cut(s) 2625, 3252, 3435
BveI ACCTGC 1 cut(s) 3091
CaiI CAGNNNCTG 4 cut(s) 230, 2990, 3911, 4037
CciI TCATGA 2 cut(s) 955, 2551
CfoI GCGC 3 cut(s) 92, 1830, 4097
Cfr10I RCCGGY 1 cut(s) 1891
ClaI ATCGAT 1 cut(s) 1674
CseI GACGC 1 cut(s) 2160
Csp6I GTAC 7 cut(s) 498, 1210, 1509, 1624, 1753, 3412, 3950
CspAI ACCGGT 1 cut(s) 1891
CspCI CAANNNNNGTGG 4 cut(s) 892, 927, 3781, 3816
CviQI GTAC 7 cut(s) 498, 1210, 1509, 1624, 1753, 3412, 3950
DraIII CACNNNGTG 2 cut(s) 245, 3962
DrdI GACNNNNNNGTC 1 cut(s) 3599
DseDI GACNNNNNNGTC 1 cut(s) 3599
EaeI YGGCCR 2 cut(s) 1665, 3039
Eam1104I CTCTTC 4 cut(s) 1752, 1788, 3447, 3870
EarI CTCTTC 4 cut(s) 1752, 1788, 3447, 3870
Ecl136II GAGCTC 1 cut(s) 3813
Eco105I TACGTA 1 cut(s) 2870
Eco130I CCWWGG 5 cut(s) 441, 1806, 2373, 2697, 3153
Eco24I GRGCYC 7 cut(s) 102, 487, 593, 1207, 1418, 2565, 3815
Eco32I GATATC 2 cut(s) 2953, 4006
Eco47I GGWCC 9 cut(s) 55, 616, 1360, 1989, 2451, 2781, 3110, 3362, 3970
Eco47III AGCGCT 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 3813
Eco57I CTGAAG 9 cut(s) 78, 429, 1206, 1324, 2088, 2415, 3080, 3708, 3933
Eco72I CACGTG 1 cut(s) 3635
Eco81I CCTNAGG 1 cut(s) 1179
Eco91I GGTNACC 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 3813
EcoO109I RGGNCCY 2 cut(s) 616, 3940
EcoO65I GGTNACC 1 cut(s) 206
EcoRI GAATTC 2 cut(s) 1719, 2795
EcoRII CCWGG 7 cut(s) 274, 592, 1626, 1854, 2034, 2683, 3231
EcoRV GATATC 2 cut(s) 2953, 4006
EcoT14I CCWWGG 5 cut(s) 441, 1806, 2373, 2697, 3153
EcoT22I ATGCAT 4 cut(s) 535, 1277, 1390, 3120
EcoT38I GRGCYC 7 cut(s) 102, 487, 593, 1207, 1418, 2565, 3815
ErhI CCWWGG 5 cut(s) 441, 1806, 2373, 2697, 3153
Esp3I CGTCTC 1 cut(s) 2178
FalI AAGNNNNNCTT 4 cut(s) 2202, 2234, 2516, 2548
FaqI GGGAC 5 cut(s) 41, 602, 835, 2464, 2715
FauI CCCGC 2 cut(s) 1188, 2572
FauNDI CATATG 1 cut(s) 1099
FbaI TGATCA 1 cut(s) 3367
FriOI GRGCYC 7 cut(s) 102, 487, 593, 1207, 1418, 2565, 3815
GlaI GCGC 3 cut(s) 91, 1829, 4096
GsuI CTGGAG 4 cut(s) 176, 615, 2667, 3204
HaeII RGCGCY 2 cut(s) 93, 1831
HaeIII GGCC 8 cut(s) 1667, 2011, 2039, 2150, 2563, 3041, 3237, 3942
HapII CCGG 6 cut(s) 320, 1715, 1892, 2828, 3798, 3968
HgaI GACGC 1 cut(s) 2160
HhaI GCGC 3 cut(s) 92, 1830, 4097
Hin6I GCGC 3 cut(s) 90, 1828, 4095
HinP1I GCGC 3 cut(s) 90, 1828, 4095
HincII GTYRAC 1 cut(s) 3829
HindII GTYRAC 1 cut(s) 3829
HindIII AAGCTT 3 cut(s) 254, 948, 3708
HpaII CCGG 6 cut(s) 320, 1715, 1892, 2828, 3798, 3968
Hpy166II GTNNAC 2 cut(s) 1324, 3829
Hpy8I GTNNAC 2 cut(s) 1324, 3829
HpyCH4IV ACGT 2 cut(s) 2869, 3634
HpySE526I ACGT 2 cut(s) 2869, 3634
HspAI GCGC 3 cut(s) 90, 1828, 4095
Ksp22I TGATCA 1 cut(s) 3367
LguI GCTCTTC 1 cut(s) 3447
LmnI GCTCC 9 cut(s) 73, 377, 967, 1450, 2489, 3185, 3345, 3810, 4129
MaeII ACGT 2 cut(s) 2869, 3634
MaeIII GTNAC 7 cut(s) 206, 787, 1216, 1954, 2623, 3103, 3635
MbiI CCGCTC 1 cut(s) 970
MfeI CAATTG 5 cut(s) 266, 1559, 2898, 3608, 3738
MflI RGATCY 8 cut(s) 473, 1862, 1982, 2476, 2504, 3010, 3030, 3418
MhlI GDGCHC 8 cut(s) 102, 487, 593, 854, 1207, 1418, 2565, 3815
MlsI TGGCCA 1 cut(s) 1667
MluNI TGGCCA 1 cut(s) 1667
MlyI GAGTC 8 cut(s) 176, 275, 1549, 1736, 1951, 2749, 3288, 3632
MmeI TCCRAC 4 cut(s) 892, 2808, 3058, 4132
Mox20I TGGCCA 1 cut(s) 1667
Mph1103I ATGCAT 4 cut(s) 535, 1277, 1390, 3120
MroXI GAANNNNTTC 3 cut(s) 1797, 2214, 3480
MscI TGGCCA 1 cut(s) 1667
MslI CAYNNNNRTG 6 cut(s) 1059, 1317, 2409, 3264, 3375, 3573
Msp20I TGGCCA 1 cut(s) 1667
MspA1I CMGCKG 4 cut(s) 131, 3911, 4100, 4127
MspI CCGG 6 cut(s) 320, 1715, 1892, 2828, 3798, 3968
MspR9I CCNGG 8 cut(s) 276, 594, 1628, 1716, 1856, 2036, 2685, 3233
MunI CAATTG 5 cut(s) 266, 1559, 2898, 3608, 3738
Mva1269I GAATGC 4 cut(s) 1904, 3691, 3721, 3776
MvaI CCWGG 7 cut(s) 276, 594, 1628, 1856, 2036, 2685, 3233
NciI CCSGG 1 cut(s) 1716
NcoI CCATGG 2 cut(s) 2373, 3153
NdeI CATATG 1 cut(s) 1099
NheI GCTAGC 1 cut(s) 2762
NmeAIII GCCGAG 2 cut(s) 1576, 3067
NmuCI GTSAC 5 cut(s) 206, 1216, 1954, 2623, 3635
NsiI ATGCAT 4 cut(s) 535, 1277, 1390, 3120
NspI RCATGY 7 cut(s) 330, 342, 1123, 1318, 1388, 3328, 3540
OliI CACNNNNGTG 1 cut(s) 1059
PaeI GCATGC 1 cut(s) 1123
PagI TCATGA 2 cut(s) 955, 2551
PciI ACATGT 4 cut(s) 326, 338, 1314, 3324
PciSI GCTCTTC 1 cut(s) 3447
PctI GAATGC 4 cut(s) 1904, 3691, 3721, 3776
PdmI GAANNNNTTC 3 cut(s) 1797, 2214, 3480
PfeI GAWTC 6 cut(s) 140, 1082, 1529, 2099, 2858, 3623
Pfl23II CGTACG 1 cut(s) 497
PflMI CCANNNNNTGG 2 cut(s) 1409, 2887
PfoI TCCNGGA 2 cut(s) 1854, 2683
PinAI ACCGGT 1 cut(s) 1891
PleI GAGTC 8 cut(s) 175, 275, 1549, 1736, 1951, 2748, 3288, 3632
PmaCI CACGTG 1 cut(s) 3635
PmlI CACGTG 1 cut(s) 3635
PpsI GAGTC 8 cut(s) 175, 275, 1549, 1736, 1951, 2748, 3288, 3632
Ppu21I YACGTR 2 cut(s) 2870, 3635
PpuMI RGGWCCY 1 cut(s) 616
PscI ACATGT 4 cut(s) 326, 338, 1314, 3324
PshBI ATTAAT 2 cut(s) 2418, 3396
PsiI TTATAA 2 cut(s) 1653, 3218
Psp124BI GAGCTC 1 cut(s) 3815
Psp5II RGGWCCY 1 cut(s) 616
Psp6I CCWGG 7 cut(s) 274, 592, 1626, 1854, 2034, 2683, 3231
PspCI CACGTG 1 cut(s) 3635
PspEI GGTNACC 1 cut(s) 206
PspGI CCWGG 7 cut(s) 274, 592, 1626, 1854, 2034, 2683, 3231
PspLI CGTACG 1 cut(s) 497
PspOMI GGGCCC 1 cut(s) 2561
PspPPI RGGWCCY 1 cut(s) 616
PsrI GAACNNNNNNTAC 2 cut(s) 3300, 3332
PstI CTGCAG 1 cut(s) 4042
PstNI CAGNNNCTG 4 cut(s) 230, 2990, 3911, 4037
PsuI RGATCY 8 cut(s) 473, 1862, 1982, 2476, 2504, 3010, 3030, 3418
PvuII CAGCTG 3 cut(s) 131, 3911, 4100
RsaI GTAC 7 cut(s) 499, 1211, 1510, 1625, 1754, 3413, 3951
RsaNI GTAC 7 cut(s) 498, 1210, 1509, 1624, 1753, 3412, 3950
RseI CAYNNNNRTG 6 cut(s) 1059, 1317, 2409, 3264, 3375, 3573
SacI GAGCTC 1 cut(s) 3815
SapI GCTCTTC 1 cut(s) 3447
SchI GAGTC 8 cut(s) 176, 275, 1549, 1736, 1951, 2749, 3288, 3632
ScrFI CCNGG 8 cut(s) 276, 594, 1628, 1716, 1856, 2036, 2685, 3233
SduI GDGCHC 8 cut(s) 102, 487, 593, 854, 1207, 1418, 2565, 3815
SfcI CTRYAG 4 cut(s) 748, 2970, 3677, 4038
SgrAI CRCCGGYG 1 cut(s) 1891
SinI GGWCC 9 cut(s) 55, 616, 1360, 1989, 2451, 2781, 3110, 3362, 3970
SmiMI CAYNNNNRTG 6 cut(s) 1059, 1317, 2409, 3264, 3375, 3573
SmlI CTYRAG 6 cut(s) 85, 257, 1364, 2005, 2522, 2533
SmoI CTYRAG 6 cut(s) 85, 257, 1364, 2005, 2522, 2533
SnaBI TACGTA 1 cut(s) 2870
SphI GCATGC 1 cut(s) 1123
SsiI CCGC 7 cut(s) 970, 1166, 1195, 1358, 2565, 4127, 4212
SstI GAGCTC 1 cut(s) 3815
StyD4I CCNGG 8 cut(s) 274, 592, 1626, 1714, 1854, 2034, 2683, 3231
StyI CCWWGG 5 cut(s) 441, 1806, 2373, 2697, 3153
TaiI ACGT 2 cut(s) 2872, 3637
TatI WGTACW 2 cut(s) 1209, 1508
TauI GCSGC 1 cut(s) 4214
TfiI GAWTC 6 cut(s) 140, 1082, 1529, 2099, 2858, 3623
TseFI GTSAC 5 cut(s) 206, 1216, 1954, 2623, 3635
Tsp45I GTSAC 5 cut(s) 206, 1216, 1954, 2623, 3635
TspGWI ACGGA 3 cut(s) 2150, 2917, 3814
Van91I CCANNNNNTGG 2 cut(s) 1409, 2887
VpaK11BI GGWCC 9 cut(s) 55, 616, 1360, 1989, 2451, 2781, 3110, 3362, 3970
VspI ATTAAT 2 cut(s) 2418, 3396
XbaI TCTAGA 1 cut(s) 2645
XceI RCATGY 7 cut(s) 330, 342, 1123, 1318, 1388, 3328, 3540
XcmI CCANNNNNNNNNTGG 3 cut(s) 382, 1023, 2494
XmnI GAANNNNTTC 3 cut(s) 1797, 2214, 3480
Zsp2I ATGCAT 4 cut(s) 535, 1277, 1390, 3120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.