MD07G1005500.v1.1

mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005851
Physical Location & Seq
Reverse (-)
558730 .. 572334
13605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1005500.v1.1.491

Sequence Viewer

Length: 4401 bp
ATGCTCCCTCACATAAAGGGAGACTTCGCCGCCTCTCACTCTCCAGCCTCTCTCCTCTCCTCGCTCTCTACCCCTCCTAAAACCCTGCTTCCGACGTCTACCATCTCTCCGCACAGAGATATATACTTATATAATCGTTACACAGTTGCAGGTAATATATTTGTAGGGGTGACAATGGCTGGAAAGTCGAATAAAGGAAAGAACCGTAGAGGGGCGAACAATGCTGTGGTCCCATCTGATGCTCCTGTGAAAGATAACTCAAGCACTTCAGAGCCCGTTAAAGCTGAAGACAATGGTGTTCCAGCTGTGGAGGAATTAACTGATGCCAGTCTGGAGGTAAAGGAGTCTGAAACCGAAAATTCAACCGGCCAACCTAAGCAAGGTGACCTTCATCTGTATCCAGTTTCTGTCAAGACACAAAATGGCGAGAAGCTTGAGCTACAATTGAACCCAGGAGACTCTGTTATGGATATAAGACAATTTCTGCTTGATGCACCAGAGACATGTTTCTTCACATGTTATGACTTATTGCTACACACAAAGGATGGCTCCACTCATCATTTGGAAGACTTTAATGAAATTTCCGAAGTTGCTGATATTACCGTTGGTGGTTGCTCCTTGGAGATGGTTCCTGCTTTATATGATGATAGATCTATAAGGGCTCATGTTCATCGTACGAGAGAATTGCTTTCCCTTTCTACACTTCATGCATCACTATCGACATCGCTTGCATTACAGTATGAGACAGCACAAAACAAAGTGGCGAGCCCTGGAGATACTGTGAAAACTGAGGTCCCAGGGCTTGATGGTTTGGGTTTTATGGAGGATGTTGCCGGTTCATTGAGCAATTTGCTATCTTCACCCTCAAAAGAGATCAAATGTGTGGAGAGTATTGTGTTTTCATCCTTCAATCCTCCGCCAAGCTATAGAAGGCTTGTTGGGGATTTGATTTATTTGGATATAGTAACATTGGAGGGCAATAAACACTGTATTACTGGGACTACAAGAATGTTCTATGTCAATTCGAGCACGGGGAACACTCTTGACCCTAAGCCAAGTAAAAGTAATTGGGAAGCAACCACACTTGTTGGACTTTTGCAAAATATTAGCTCTAAGTTCAAAAAAGCTTTTCGTGAAATTTTGGAGCAGAGGGCATCTGCACATCCTTTTGAAAATGTTCAGTCTTTATTGCCACCAAATTCATGGCTAGGGTTATATCCAGTTCCAGATCACAGGCGTGATGCAGCTAGAGCAGAGGATGCCCTTACTCTTTCATACGTCAGTGAGCTAATTGGCATGCAAAGGGATTGGAATGAGGAGTTGCAATCTTGTAGGGAATTTCCACATACAACTCCTCAGGAAAGAATTTTGCGGGATAGGGCCCTGTACAAAGTGACTTCAGACTTTGTTGATGCAGCTATTAGTGGTGCTATTGGAGTTATTAGTAGATGCATACCTCCTATAAACCCAACTGATCCAGAGTGCTTCCACATGTATGTTCACAATAATATATTCTTCAGTTTTGCTGTTGATGCGGACCTTGAGCAGCTGTCTAAGAAACGTGTATCAAATTCAAGTCCAAAGATAGGGGGCACTGGTTCTGTACACAGTTCTTCTGAGAAGGCTACTGACAATTTGTTGCATGGAGAGAATGCAATTCCCAATAGAGAAAAATGCAAAGGATCAAGTATAATTGATGATGCCACAGAATCCTCCTCTGATGTTTCTGCCGAGACACAATTGGGTGAAACCGAGCAGGCTACTTATGCTTCTGCAAATAATGATTTGAAAGGCACCAAGGCGTACCAGGAAGCTGATGTCTCTGGACTTTATAATCTTGCTATGGCCATAATCGATTACAGGGGCCATAGAGTAGTAGCTCAGAGTGTTTTACCGGGCATTCTTCAAGGGGATAAATCAGACTCTCTTCTATATGGTTCTGTTGATAATGGCAAGAAAATATGCTGGAATGAAGAGTTCCATTCCAAGGTGCTGGAAGCTGCTAAGCGCCTTCATCTTAAAGAGCACACAGTCCTTGATGGATCTGGAAATGTTTTCAGATTGGCTGCACCGGTGGAATGCAAGGGTATTGTTGGTAGTGATGACAGGCATTATCTCCTCGACTTGATGAGAGTGACTCCTCGTGATTCAAACTGCACTGGACCTGGGTCCAGATTTTGTATCTTGAGGCTGGAATTAATTACTGCTTATTGCCAGGCCCAAGCTGCGGAGAAACCAAAATCTAAGTCTAAAGATGGAGAAGGTCTTGTCACCACTGATTCTTCAGTCATCACTGATGCTAAGCAGGACATAACGAAAGAAGGGAATGCCACAGACGCACAAGAAATTGCATCTCCACCTCCATCCACTGATAGCTCTGATCCATGTGAAGAAATTCTTTTTAACCCTAATGTCTTCACCGAGTTTAAGTTGGCTGGAAATGAGGAGGAGATAGCAGAAGATGAAGGAAATGTGAGGAAAGCCAGTTTGTATCTTTCAGATGTTGTGCTTCCAAAATTTATACAAGACCTCTGCACGCTTGAAGTATCACCCATGGACGGGCAGACGCTAACTGAAGCACTCCATGCTCATGGAATTAACGTTCGCTATATTGGAAAAGTGGCTGAGGGGACCAAACATTTACCTCATCTTTGGGATCTTTGCTCCAATGAGATAGTGGTTAGATCTGCTAAGCACATTCTCAAGGATGCCTTGAGAGAAACAAATGATCATGATATTGGGCCTGCAATCACCCATTTCTTTAACTGTTTCTTTGGAAGCTGTCAAGCCGTTGGCCCAAAAGTTGCTGCTAATAATATGCAGTCTAGAACGCCCAAAAAGGAACAAAAAGGACAGCAATCTCCAAGAAAATTGTCTAAGGGACAAGGAAAATTGAAGGATGGAGCATCTGCTAGAAAGAGTCGATCCTCATTTATGCTTGCTAGCTCAGAAACTCTATGGTTCGACATTCAAGAATTCGCAAAACTCAAATATCAGTTTGAGCTGCCGGAGGATGCTAGGATGCGTGTAAAGAAAGATTCTGTTATACGTAATCTTTGCCAAAAGGTGGGCATTACAATTGCAGCTCGCAGATATGATCTTAATTCTGCAGCACCTTTCCAAATGTCAGATATCTTAAATCTTCAACCTGTAGTGAAACATTCAGTTCCTGTCTGTTCAGAAGCTAAGGATCTTGTGGAAACAGGAAAGATTCAACTGGCCGAGGGAATGTTAAGTGAAGCCTACACATTATTTACCGAGGCATTTTCAATACTTCAGCAGGTTACTGGTCCAATGCATAGGGAGGTTGCTAACTGCTGTCGGTATCTTGCCATGGTTCTGTATCATGCAGGAGACATGGCTGGAGCCATAATGCAACAGCATAAGGAACTAATTATAAATGAACGCTGCCTTGGCCTAGACCACCCTGACACTGCTCACAGCTATGGAAATATGGCTCTTTTCTACCATGGACTTAACCAGACAGAACTTGCACTACGGCACATGTCGAGGGCATTGCTTCTGCTAAGTTTATCATCTGGCCCAGATCACCCTGATGTTGCTGCAACTTTCATTAATGTCGCTATGATGTACCAGGATCTTGGGAAGATGGACACTGCTCTTCGATATTTGCAAGAGGCTCTGAAAAAAAATGAAAGGCTTCTAGGGGAGGAGCACATCCAGACTGCTGTATGCTATCACGCACTTGCTATTGCATTTAATTGTATGGGTGCCTTCAAGCTCTCTCACCAGCATGAGAAGAAAACTTATGACATACTTGTCAAACAATTGGGCGAGGAGGATTCTAGAACACGTGACTCTCAAAACTGGATGAAGACATTTAAGATGCGTGAGCTACAGATGAATGCACAAAAGCAAAAAGGTCAAGCTTTGAATGCTGCTTCGGCTCAAAAGGCAATTGACATCTTGAAGGCCCATCCTGACTTGATGCAGGCATTCCAATCTGCTGCCATAGCCGGTGGGTCTGGGAGCTCTAACCCTTCCGCCAACAAATCTCTTAATGCTGCAATAATTGGTGAAACCCTTCCACGAGGTAGGGGAGTTGATGAGCGGGCTGCTCGTGCAGCAGCTGAAGTCAGGAAGAAGGCAGCAGCAAAGGGCCTCTTGATACGCCCACACGGTGTTCCCGTCCAAGCTGTGCCTCCACTTCCTCAGTTACTCGATATCATCAATTCAGGTGCAACCCCACCGGTTGCAGTGGAAAATAAAGAAACTGATGGAGTGAAGGAAGCCAATGGTCATCCTGCAAATGGCTTAACTGATGTGAAACAAGAGCAATCAACAACGGAGCAGGAAGGTCAGCCTCCAGTTGGATTGGGCAAAGGTTTAGCAACACTGGACGGCAAGAAACAGAAATCGAAAACCAAAGCCGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N DUF727 TPR_10 TPR_12 eIF3_p135

Protein Analysis

1467

Amino Acids

160.2

Weight (kDa)

6.06

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 153 - 229 1.3e-14 Mitochondrial function, CLU-N-term
CLU PF13236 436 - 718 4.2e-73 Clustered mitochondria
eIF3_p135 PF12807 849 - 1033 1.8e-47 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 1110 - 1173 1.3e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 1146 - 1287 6.8e-13 Nephrocystin-3 TPR domain
TPR_12 PF13424 1149 - 1216 1.1e-11 Tetratricopeptide repeat
TPR_12 PF13424 1186 - 1259 6.5e-14 Tetratricopeptide repeat
TPR_10 PF13374 1187 - 1227 1.1e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1833, 3398
AasI GACNNNNNNGTC 1 cut(s) 3779
AatII GACGTC 1 cut(s) 98
Acc36I ACCTGC 2 cut(s) 140, 3271
AccB1I GGYRCC 2 cut(s) 1793, 3731
AccB7I CCANNNNNTGG 1 cut(s) 3067
AccBSI CCGCTC 1 cut(s) 4072
AccI GTMKAC 1 cut(s) 98
AciI CCGC 9 cut(s) 30, 110, 917, 1372, 1535, 2228, 4005, 4072, 4392
AclI AACGTT 1 cut(s) 2601
AclWI GGATC 8 cut(s) 1469, 1690, 2050, 2375, 2664, 2919, 3198, 3606
AcoI YGGCCR 3 cut(s) 367, 1845, 3219
AcuI CTGAAG 8 cut(s) 252, 306, 1383, 1501, 2268, 2595, 3260, 4113
AcvI CACGTG 1 cut(s) 3815
AcyI GRCGYC 1 cut(s) 95
AdeI CACNNNGTG 1 cut(s) 4142
AfaI GTAC 5 cut(s) 676, 1388, 1605, 1805, 3593
AflIII ACRYGT 6 cut(s) 503, 515, 1491, 1561, 3504, 3812
AgeI ACCGGT 2 cut(s) 2071, 4210
AjnI CCWGG 7 cut(s) 451, 769, 796, 1806, 2164, 2214, 3594
AjuI GAANNNNNNNTTGG 2 cut(s) 3396, 3428
AleI CACNNNNGTG 1 cut(s) 1236
AloI GAACNNNNNNTCC 2 cut(s) 2586, 2618
Alw21I GWGCWC 4 cut(s) 1031, 2028, 3678, 3995
Alw26I GTCTC 7 cut(s) 15, 450, 494, 737, 1727, 1825, 3348
AlwI GGATC 8 cut(s) 1469, 1690, 2050, 2375, 2664, 2919, 3198, 3606
AlwNI CAGNNNCTG 3 cut(s) 407, 3170, 4091
ApaI GGGCCC 1 cut(s) 1384
ArsI GACNNNNNNTTYG 2 cut(s) 2231, 2263
AseI ATTAAT 2 cut(s) 2198, 3576
AsiGI ACCGGT 2 cut(s) 2071, 4210
Asp700I GAANNNNTTC 4 cut(s) 1176, 2394, 3660, 4044
AspLEI GCGC 1 cut(s) 2010
AsuC2I CCSGG 1 cut(s) 1896
AsuNHI GCTAGC 1 cut(s) 2942
AvaII GGWCC 7 cut(s) 229, 793, 1537, 2162, 2169, 2631, 3290
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 2 cut(s) 1384, 1595
BalI TGGCCA 1 cut(s) 1847
BanI GGYRCC 2 cut(s) 1793, 3731
BanII GRGCYC 5 cut(s) 276, 664, 770, 1384, 3995
BauI CACGAG 3 cut(s) 2142, 4050, 4080
BbrPI CACGTG 1 cut(s) 3815
BbsI GAAGAC 4 cut(s) 294, 573, 2407, 3842
Bbv12I GWGCWC 4 cut(s) 1031, 2028, 3678, 3995
BbvCI CCTCAGC 1 cut(s) 2625
BceAI ACGGC 3 cut(s) 2774, 3515, 4378
BcgI CGANNNNNNTGC 6 cut(s) 667, 699, 701, 733, 3499, 3533
BciT130I CCWGG 7 cut(s) 453, 771, 798, 1808, 2166, 2216, 3596
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 1 cut(s) 2728
BcnI CCSGG 1 cut(s) 1896
BcoDI GTCTC 7 cut(s) 15, 450, 494, 737, 1727, 1825, 3348
BfaI CTAG 9 cut(s) 1208, 1248, 2826, 2913, 2943, 3018, 3419, 3665, 3807
BfmI CTRYAG 4 cut(s) 925, 3108, 3150, 3857
BfoI RGCGCY 1 cut(s) 2011
BfuAI ACCTGC 2 cut(s) 140, 3271
BfuI GTATCC 1 cut(s) 408
BglII AGATCT 2 cut(s) 650, 2684
BlpI GCTNAGC 3 cut(s) 2004, 2301, 2691
Bme1390I CCNGG 8 cut(s) 453, 771, 798, 1808, 1896, 2166, 2216, 3596
Bme18I GGWCC 7 cut(s) 229, 793, 1537, 2162, 2169, 2631, 3290
BmrFI CCNGG 8 cut(s) 453, 771, 798, 1808, 1896, 2166, 2216, 3596
BmrI ACTGGG 1 cut(s) 1005
BmtI GCTAGC 1 cut(s) 2946
BmuI ACTGGG 1 cut(s) 1005
BoxI GACNNNNGTC 1 cut(s) 2167
BpiI GAAGAC 4 cut(s) 294, 573, 2407, 3842
BplI GAGNNNNNCTC 2 cut(s) 2122, 2154
BpmI CTGGAG 5 cut(s) 27, 353, 792, 3384, 4311
Bpu10I CCTNAGC 4 cut(s) 375, 1050, 2625, 3186
Bpu1102I GCTNAGC 3 cut(s) 2004, 2301, 2691
BpuEI CTTGAG 6 cut(s) 244, 455, 1562, 2206, 2687, 2734
BpuMI CCSGG 1 cut(s) 1896
Bsa29I ATCGAT 1 cut(s) 1854
BsaAI YACGTR 2 cut(s) 3050, 3815
BsaHI GRCGYC 1 cut(s) 95
BsaWI WCCGGW 2 cut(s) 2071, 4210
BsaXI ACNNNNNCTCC 6 cut(s) 91, 121, 447, 477, 765, 795
Bse118I RCCGGY 5 cut(s) 365, 833, 2071, 3977, 4210
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 1 cut(s) 3515
BseBI CCWGG 7 cut(s) 453, 771, 798, 1808, 2166, 2216, 3596
BseCI ATCGAT 1 cut(s) 1854
BseMI GCAATG 1 cut(s) 3515
BseMII CTCAG 7 cut(s) 780, 1370, 1608, 1895, 2616, 2961, 4187
BseSI GKGCMC 2 cut(s) 1384, 1595
BsgI GTGCAG 5 cut(s) 1143, 2052, 2140, 2518, 4104
BshNI GGYRCC 2 cut(s) 1793, 3731
BshTI ACCGGT 2 cut(s) 2071, 4210
BshVI ATCGAT 1 cut(s) 1854
BsiHKAI GWGCWC 4 cut(s) 1031, 2028, 3678, 3995
BsiSI CCGG 7 cut(s) 366, 834, 1895, 2072, 3008, 3978, 4211
BsiWI CGTACG 1 cut(s) 674
BslFI GGGAC 5 cut(s) 215, 779, 1012, 2644, 2895
BsmAI GTCTC 7 cut(s) 15, 450, 494, 737, 1727, 1825, 3348
BsmFI GGGAC 5 cut(s) 215, 779, 1012, 2644, 2895
BsmI GAATGC 7 cut(s) 1657, 1899, 2084, 2332, 3871, 3901, 3956
Bsp120I GGGCCC 1 cut(s) 1380
Bsp1286I GDGCHC 9 cut(s) 276, 664, 770, 1031, 1384, 1595, 2028, 3678, 3995
Bsp1407I TGTACA 2 cut(s) 1386, 1603
Bsp1720I GCTNAGC 3 cut(s) 2004, 2301, 2691
Bsp19I CCATGG 3 cut(s) 2553, 3333, 3469
BspACI CCGC 9 cut(s) 30, 110, 917, 1372, 1535, 2228, 4005, 4072, 4392
BspCNI CTCAG 7 cut(s) 781, 1369, 1609, 1894, 2617, 2960, 4186
BspDI ATCGAT 1 cut(s) 1854
BspHI TCATGA 1 cut(s) 2731
BspMAI CTGCAG 1 cut(s) 3112
BspMI ACCTGC 2 cut(s) 140, 3271
BspOI GCTAGC 1 cut(s) 2946
BspPI GGATC 8 cut(s) 1469, 1690, 2050, 2375, 2664, 2919, 3198, 3606
BspQI GCTCTTC 1 cut(s) 3627
BspT107I GGYRCC 2 cut(s) 1793, 3731
BsrBI CCGCTC 1 cut(s) 4072
BsrDI GCAATG 1 cut(s) 3515
BsrFI RCCGGY 5 cut(s) 365, 833, 2071, 3977, 4210
BsrGI TGTACA 2 cut(s) 1386, 1603
BssAI RCCGGY 5 cut(s) 365, 833, 2071, 3977, 4210
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 3 cut(s) 2142, 4050, 4080
BssT1I CCWWGG 7 cut(s) 618, 1797, 1986, 2553, 3333, 3412, 3469
Bst2BI CACGAG 3 cut(s) 2142, 4050, 4080
Bst2UI CCWGG 7 cut(s) 453, 771, 798, 1808, 2166, 2216, 3596
Bst6I CTCTTC 3 cut(s) 1932, 1968, 3627
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 2 cut(s) 1259, 2585
BstAUI TGTACA 2 cut(s) 1386, 1603
BstBAI YACGTR 2 cut(s) 3050, 3815
BstDSI CCRYGG 3 cut(s) 2553, 3333, 3469
BstEII GGTNACC 1 cut(s) 383
BstENI CCTNNNNNAGG 1 cut(s) 378
BstH2I RGCGCY 1 cut(s) 2011
BstHHI GCGC 1 cut(s) 2010
BstMAI GTCTC 7 cut(s) 15, 450, 494, 737, 1727, 1825, 3348
BstNI CCWGG 7 cut(s) 453, 771, 798, 1808, 2166, 2216, 3596
BstNSI RCATGY 5 cut(s) 507, 519, 1300, 1495, 3508
BstPAI GACNNNNGTC 1 cut(s) 2167
BstPI GGTNACC 1 cut(s) 383
BstSCI CCNGG 8 cut(s) 451, 769, 796, 1806, 1894, 2164, 2214, 3594
BstSFI CTRYAG 4 cut(s) 925, 3108, 3150, 3857
BstSLI GKGCMC 2 cut(s) 1384, 1595
BstSNI TACGTA 1 cut(s) 3050
BstV2I GAAGAC 4 cut(s) 294, 573, 2407, 3842
BstX2I RGATCY 6 cut(s) 650, 2042, 2656, 2684, 3190, 3598
BstXI CCANNNNNNTGG 4 cut(s) 1203, 1993, 2591, 3602
BstYI RGATCY 6 cut(s) 650, 2042, 2656, 2684, 3190, 3598
Bsu15I ATCGAT 1 cut(s) 1854
Bsu36I CCTNAGG 1 cut(s) 1356
BsuI GTATCC 1 cut(s) 408
BsuTUI ATCGAT 1 cut(s) 1854
BtgI CCRYGG 3 cut(s) 2553, 3333, 3469
BtgZI GCGATG 1 cut(s) 708
BtsI GCAGTG 3 cut(s) 3432, 3615, 4224
BveI ACCTGC 2 cut(s) 140, 3271
CaiI CAGNNNCTG 3 cut(s) 407, 3170, 4091
CciI TCATGA 1 cut(s) 2731
CfoI GCGC 1 cut(s) 2010
Cfr10I RCCGGY 5 cut(s) 365, 833, 2071, 3977, 4210
ClaI ATCGAT 1 cut(s) 1854
CseI GACGC 2 cut(s) 2345, 2575
Csp6I GTAC 5 cut(s) 675, 1387, 1604, 1804, 3592
CspAI ACCGGT 2 cut(s) 2071, 4210
CspCI CAANNNNNGTGG 2 cut(s) 1069, 1104
CviQI GTAC 5 cut(s) 675, 1387, 1604, 1804, 3592
DraIII CACNNNGTG 1 cut(s) 4142
DrdI GACNNNNNNGTC 1 cut(s) 3779
DseDI GACNNNNNNGTC 1 cut(s) 3779
EaeI YGGCCR 3 cut(s) 367, 1845, 3219
Eam1104I CTCTTC 3 cut(s) 1932, 1968, 3627
EarI CTCTTC 3 cut(s) 1932, 1968, 3627
EciI GGCGGA 2 cut(s) 906, 3994
Ecl136II GAGCTC 1 cut(s) 3993
Eco105I TACGTA 1 cut(s) 3050
Eco130I CCWWGG 7 cut(s) 618, 1797, 1986, 2553, 3333, 3412, 3469
Eco24I GRGCYC 5 cut(s) 276, 664, 770, 1384, 3995
Eco32I GATATC 2 cut(s) 3133, 4186
Eco47I GGWCC 7 cut(s) 229, 793, 1537, 2162, 2169, 2631, 3290
Eco53kI GAGCTC 1 cut(s) 3993
Eco57I CTGAAG 8 cut(s) 252, 306, 1383, 1501, 2268, 2595, 3260, 4113
Eco72I CACGTG 1 cut(s) 3815
Eco81I CCTNAGG 1 cut(s) 1356
Eco91I GGTNACC 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 3993
EcoNI CCTNNNNNAGG 1 cut(s) 378
EcoO109I RGGNCCY 4 cut(s) 793, 1380, 1381, 4120
EcoO65I GGTNACC 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 2975
EcoRII CCWGG 7 cut(s) 451, 769, 796, 1806, 2164, 2214, 3594
EcoRV GATATC 2 cut(s) 3133, 4186
EcoT14I CCWWGG 7 cut(s) 618, 1797, 1986, 2553, 3333, 3412, 3469
EcoT22I ATGCAT 3 cut(s) 712, 1454, 3300
EcoT38I GRGCYC 5 cut(s) 276, 664, 770, 1384, 3995
ErhI CCWWGG 7 cut(s) 618, 1797, 1986, 2553, 3333, 3412, 3469
FaqI GGGAC 5 cut(s) 215, 779, 1012, 2644, 2895
FauI CCCGC 2 cut(s) 1365, 4065
FbaI TGATCA 1 cut(s) 2728
FblI GTMKAC 1 cut(s) 98
FriOI GRGCYC 5 cut(s) 276, 664, 770, 1384, 3995
FspBI CTAG 9 cut(s) 1208, 1248, 2826, 2913, 2943, 3018, 3419, 3665, 3807
GlaI GCGC 1 cut(s) 2009
GsuI CTGGAG 5 cut(s) 27, 353, 792, 3384, 4311
HaeII RGCGCY 1 cut(s) 2011
HapII CCGG 7 cut(s) 366, 834, 1895, 2072, 3008, 3978, 4211
HgaI GACGC 2 cut(s) 2345, 2575
HhaI GCGC 1 cut(s) 2010
Hin1I GRCGYC 1 cut(s) 95
Hin6I GCGC 1 cut(s) 2008
HinP1I GCGC 1 cut(s) 2008
HindIII AAGCTT 3 cut(s) 431, 1125, 3888
HpaII CCGG 7 cut(s) 366, 834, 1895, 2072, 3008, 3978, 4211
Hpy166II GTNNAC 3 cut(s) 99, 1501, 1606
Hpy8I GTNNAC 3 cut(s) 99, 1501, 1606
Hpy99I CGWCG 1 cut(s) 97
HpyCH4IV ACGT 6 cut(s) 95, 1278, 1561, 2601, 3049, 3814
HpySE526I ACGT 6 cut(s) 95, 1278, 1561, 2601, 3049, 3814
Hsp92I GRCGYC 1 cut(s) 95
HspAI GCGC 1 cut(s) 2008
Ksp22I TGATCA 1 cut(s) 2728
LguI GCTCTTC 1 cut(s) 3627
MaeI CTAG 9 cut(s) 1208, 1248, 2826, 2913, 2943, 3018, 3419, 3665, 3807
MaeII ACGT 6 cut(s) 95, 1278, 1561, 2601, 3049, 3814
MbiI CCGCTC 1 cut(s) 4072
MfeI CAATTG 5 cut(s) 443, 1739, 3078, 3788, 3918
MflI RGATCY 6 cut(s) 650, 2042, 2656, 2684, 3190, 3598
MhlI GDGCHC 9 cut(s) 276, 664, 770, 1031, 1384, 1595, 2028, 3678, 3995
MlsI TGGCCA 1 cut(s) 1847
MluNI TGGCCA 1 cut(s) 1847
MlyI GAGTC 6 cut(s) 353, 452, 1916, 2131, 2929, 3812
MmeI TCCRAC 3 cut(s) 116, 1069, 4312
Mox20I TGGCCA 1 cut(s) 1847
Mph1103I ATGCAT 3 cut(s) 712, 1454, 3300
MroXI GAANNNNTTC 4 cut(s) 1176, 2394, 3660, 4044
MscI TGGCCA 1 cut(s) 1847
MslI CAYNNNNRTG 6 cut(s) 1236, 1494, 2589, 3444, 3555, 3753
Msp20I TGGCCA 1 cut(s) 1847
MspA1I CMGCKG 3 cut(s) 305, 1549, 4091
MspI CCGG 7 cut(s) 366, 834, 1895, 2072, 3008, 3978, 4211
MspR9I CCNGG 8 cut(s) 453, 771, 798, 1808, 1896, 2166, 2216, 3596
MunI CAATTG 5 cut(s) 443, 1739, 3078, 3788, 3918
Mva1269I GAATGC 7 cut(s) 1657, 1899, 2084, 2332, 3871, 3901, 3956
MvaI CCWGG 7 cut(s) 453, 771, 798, 1808, 2166, 2216, 3596
NciI CCSGG 1 cut(s) 1896
NcoI CCATGG 3 cut(s) 2553, 3333, 3469
NheI GCTAGC 1 cut(s) 2942
NmeAIII GCCGAG 2 cut(s) 1756, 3247
NmuCI GTSAC 6 cut(s) 169, 383, 1393, 2134, 2269, 3815
NsiI ATGCAT 3 cut(s) 712, 1454, 3300
NspI RCATGY 5 cut(s) 507, 519, 1300, 1495, 3508
OliI CACNNNNGTG 1 cut(s) 1236
PaeI GCATGC 1 cut(s) 1300
PagI TCATGA 1 cut(s) 2731
PasI CCCWGGG 1 cut(s) 797
PciI ACATGT 4 cut(s) 503, 515, 1491, 3504
PciSI GCTCTTC 1 cut(s) 3627
PcsI WCGNNNNNNNCGW 1 cut(s) 4146
PctI GAATGC 7 cut(s) 1657, 1899, 2084, 2332, 3871, 3901, 3956
PdmI GAANNNNTTC 4 cut(s) 1176, 2394, 3660, 4044
PfeI GAWTC 6 cut(s) 1709, 2147, 2279, 3038, 3211, 3803
Pfl23II CGTACG 1 cut(s) 674
PflMI CCANNNNNTGG 1 cut(s) 3067
PinAI ACCGGT 2 cut(s) 2071, 4210
PleI GAGTC 6 cut(s) 352, 452, 1916, 2131, 2928, 3812
PmaCI CACGTG 1 cut(s) 3815
PmlI CACGTG 1 cut(s) 3815
PpsI GAGTC 6 cut(s) 352, 452, 1916, 2131, 2928, 3812
Ppu21I YACGTR 2 cut(s) 3050, 3815
PpuMI RGGWCCY 1 cut(s) 793
PscI ACATGT 4 cut(s) 503, 515, 1491, 3504
PshAI GACNNNNGTC 1 cut(s) 2167
PshBI ATTAAT 2 cut(s) 2198, 3576
PsiI TTATAA 2 cut(s) 1833, 3398
Psp124BI GAGCTC 1 cut(s) 3995
Psp1406I AACGTT 1 cut(s) 2601
Psp5II RGGWCCY 1 cut(s) 793
Psp6I CCWGG 7 cut(s) 451, 769, 796, 1806, 2164, 2214, 3594
PspCI CACGTG 1 cut(s) 3815
PspEI GGTNACC 1 cut(s) 383
PspGI CCWGG 7 cut(s) 451, 769, 796, 1806, 2164, 2214, 3594
PspLI CGTACG 1 cut(s) 674
PspOMI GGGCCC 1 cut(s) 1380
PspPPI RGGWCCY 1 cut(s) 793
PsrI GAACNNNNNNTAC 2 cut(s) 3480, 3512
PstI CTGCAG 1 cut(s) 3112
PstNI CAGNNNCTG 3 cut(s) 407, 3170, 4091
PsuI RGATCY 6 cut(s) 650, 2042, 2656, 2684, 3190, 3598
PvuII CAGCTG 3 cut(s) 305, 1549, 4091
RsaI GTAC 5 cut(s) 676, 1388, 1605, 1805, 3593
RsaNI GTAC 5 cut(s) 675, 1387, 1604, 1804, 3592
RseI CAYNNNNRTG 6 cut(s) 1236, 1494, 2589, 3444, 3555, 3753
SacI GAGCTC 1 cut(s) 3995
SapI GCTCTTC 1 cut(s) 3627
SchI GAGTC 6 cut(s) 353, 452, 1916, 2131, 2929, 3812
ScrFI CCNGG 8 cut(s) 453, 771, 798, 1808, 1896, 2166, 2216, 3596
SduI GDGCHC 9 cut(s) 276, 664, 770, 1031, 1384, 1595, 2028, 3678, 3995
SfcI CTRYAG 4 cut(s) 925, 3108, 3150, 3857
SgrAI CRCCGGYG 1 cut(s) 2071
SinI GGWCC 7 cut(s) 229, 793, 1537, 2162, 2169, 2631, 3290
SmiMI CAYNNNNRTG 6 cut(s) 1236, 1494, 2589, 3444, 3555, 3753
SmlI CTYRAG 6 cut(s) 259, 434, 1541, 2185, 2702, 2713
SmoI CTYRAG 6 cut(s) 259, 434, 1541, 2185, 2702, 2713
SnaBI TACGTA 1 cut(s) 3050
SphI GCATGC 1 cut(s) 1300
SsiI CCGC 9 cut(s) 30, 110, 917, 1372, 1535, 2228, 4005, 4072, 4392
SspI AATATT 1 cut(s) 1105
SspMI CTAG 9 cut(s) 1208, 1248, 2826, 2913, 2943, 3018, 3419, 3665, 3807
SstI GAGCTC 1 cut(s) 3995
StyD4I CCNGG 8 cut(s) 451, 769, 796, 1806, 1894, 2164, 2214, 3594
StyI CCWWGG 7 cut(s) 618, 1797, 1986, 2553, 3333, 3412, 3469
TaiI ACGT 6 cut(s) 98, 1281, 1564, 2604, 3052, 3817
TatI WGTACW 2 cut(s) 1386, 1603
TauI GCSGC 2 cut(s) 32, 4394
TfiI GAWTC 6 cut(s) 1709, 2147, 2279, 3038, 3211, 3803
TseFI GTSAC 6 cut(s) 169, 383, 1393, 2134, 2269, 3815
Tsp45I GTSAC 6 cut(s) 169, 383, 1393, 2134, 2269, 3815
TspGWI ACGGA 1 cut(s) 4322
Van91I CCANNNNNTGG 1 cut(s) 3067
VpaK11BI GGWCC 7 cut(s) 229, 793, 1537, 2162, 2169, 2631, 3290
VspI ATTAAT 2 cut(s) 2198, 3576
XagI CCTNNNNNAGG 1 cut(s) 378
XbaI TCTAGA 2 cut(s) 2825, 3806
XceI RCATGY 5 cut(s) 507, 519, 1300, 1495, 3508
XcmI CCANNNNNNNNNTGG 3 cut(s) 559, 1200, 2674
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 4 cut(s) 1176, 2394, 3660, 4044
XspI CTAG 9 cut(s) 1208, 1248, 2826, 2913, 2943, 3018, 3419, 3665, 3807
ZraI GACGTC 1 cut(s) 96
Zsp2I ATGCAT 3 cut(s) 712, 1454, 3300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.