FvH4_4g15240

Vacuole membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002021
Physical Location & Seq
Reverse (-)
18835271 .. 18839744
4474 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15240.t2

Sequence Viewer

Length: 1086 bp
ATGTCGTATTTGTTGGCTAGACGTGGCTGGCTCATGCTGTTGAGTGCTGTGGCATCATCTCTTGGAATTCTTCTAGCGACCATTGACGGCCCCCATGAGAAACATTTCGAGGAGCTTTTCAAATATTTCCAATTTGGGTTATGGTGGGTGGCCCTTGGAGTTGCATCTTCGATTGGTCTTGGATCGGGTTTGCACACCTTTGTCCTATATCTGGGTCCTCATATTGCATTCTTTACTATAAAGGCTATGCAGTGTGGCCGAGTTGATTTAAAAAGTGCTCCTTATGATACAATACAACTATATAGTGGTCCTTCATGGCTTGACAAAAACTGCTCTGAGTTTGGTGCCCCATTGTTTTCTTCATCGCATGGATTGCGGGTTCCACTAAGCAGCATATTACCACAAGTCCAATTAGAGGCTGTTTTATGGGGTATTGGGACTGCAATTGGAGAACTTCCTCCATATTTCATCTCAAGAGCAGCAAGCTTAGCTGGTAACCAGATCGATGAATTGAAAGAATTGGATACCTCCTCAGATGAAGTAAAAGGGTACATAGCTACTCGCCTATATCAAATCAAACGCTGGCTTCTGTCACACTCGCAGCAATTGAACTTCTTTACAATCTTAGTGCTTGCTTCGGTGCCAAATCCTCTTTTTGACCTAGCAGGCATTATGTGTGGACAATTTGGGATTCCATTTTGGAAATTCTTTCTGGCAACAGTGATTGGAAAGGCTATTATTAAAACCCACCTGCAGACCGTTTTCATTATCTCAGTTTGCAACAATCAACTTCTAAATTGGATAGAGAATGAATTGATTTGGGTATTCAGTTTCATACCTGGTTTTGCTGCTGTCCTGCCCAACCTCATTGCTAAAATTGATGGAGCAAAAGAGAAGTATTTGACAGCTTCTTACTCCCGATCTCCAAATATCAAGGTGAAAACGTGGGATTTCTCATTTGCATCAATCTGGAACACTTTGGTATGGCTTATGCTATTGAACTTTTTTGTCAAGATTGTCACTTCAATGGCCCAGAGTTATCGGAAGAAAGAGCAGGAGAAGGAGCTTGCTTTGTTGACAAGTTAG

Protein Analysis

362

Amino Acids

40.45

Weight (kDa)

8.74

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 134 - 235 1.6e-07 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 759
Acc36I ACCTGC 1 cut(s) 759
AccB1I GGYRCC 2 cut(s) 344, 640
AciI CCGC 1 cut(s) 376
AclWI GGATC 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 2 cut(s) 66, 704
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 2 cut(s) 211, 415
AgsI TTSAA 5 cut(s) 121, 514, 610, 1000, 1026
AjiI CACGTC 1 cut(s) 23
AjnI CCWGG 1 cut(s) 838
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
AluBI AGCT 6 cut(s) 115, 486, 491, 557, 908, 1066
AluI AGCT 6 cut(s) 115, 486, 491, 557, 908, 1066
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 1 cut(s) 190
AoxI GGCC 4 cut(s) 88, 150, 256, 1029
ApeKI GCWGC 4 cut(s) 390, 479, 601, 848
ApoI RAATTY 2 cut(s) 66, 704
Asp700I GAANNNNTTC 1 cut(s) 104
AspS9I GGNCC 5 cut(s) 89, 151, 215, 308, 1030
AsuHPI GGTGA 1 cut(s) 949
AvaII GGWCC 2 cut(s) 215, 308
BaeGI GKGCMC 1 cut(s) 349
BanI GGYRCC 2 cut(s) 344, 640
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 4 cut(s) 402, 491, 613, 835
BccI CCATC 1 cut(s) 875
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 840
BciVI GTATCC 1 cut(s) 517
BfaI CTAG 3 cut(s) 18, 74, 662
BfmI CTRYAG 1 cut(s) 752
BfuAI ACCTGC 1 cut(s) 759
BfuI GTATCC 1 cut(s) 517
BisI GCNGC 4 cut(s) 391, 480, 602, 849
BlpI GCTNAGC 1 cut(s) 487
BlsI GCNGC 4 cut(s) 392, 481, 603, 850
Bme1390I CCNGG 1 cut(s) 840
Bme18I GGWCC 2 cut(s) 215, 308
BmgBI CACGTC 1 cut(s) 23
BmgT120I GGNCC 5 cut(s) 89, 151, 215, 308, 1030
BmiI GGNNCC 5 cut(s) 91, 216, 346, 381, 642
BmrFI CCNGG 1 cut(s) 840
BmsI GCATC 3 cut(s) 62, 173, 971
Bpu1102I GCTNAGC 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 457
Bsa29I ATCGAT 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 154
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 415
Bse3DI GCAATG 1 cut(s) 867
BseBI CCWGG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 154
BseLI CCNNNNNNNGG 2 cut(s) 211, 415
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 3 cut(s) 327, 546, 786
BseRI GAGGAG 2 cut(s) 125, 520
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 4 cut(s) 402, 491, 613, 835
BshFI GGCC 4 cut(s) 90, 152, 258, 1031
BshNI GGYRCC 2 cut(s) 344, 640
BshVI ATCGAT 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 280
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 2 cut(s) 211, 415
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 227
BsnI GGCC 4 cut(s) 90, 152, 258, 1031
Bsp1286I GDGCHC 2 cut(s) 280, 349
Bsp143I GATC 3 cut(s) 182, 501, 920
Bsp1720I GCTNAGC 1 cut(s) 487
BspACI CCGC 1 cut(s) 376
BspANI GGCC 4 cut(s) 90, 152, 258, 1031
BspCNI CTCAG 3 cut(s) 328, 545, 785
BspDI ATCGAT 1 cut(s) 504
BspLI GGNNCC 5 cut(s) 91, 216, 346, 381, 642
BspMAI CTGCAG 1 cut(s) 756
BspMI ACCTGC 1 cut(s) 759
BspPI GGATC 1 cut(s) 190
BspT107I GGYRCC 2 cut(s) 344, 640
BsrDI GCAATG 1 cut(s) 867
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 182, 501, 920
BssT1I CCWWGG 1 cut(s) 154
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 2 cut(s) 721, 760
BstAPI GCANNNNNTGC 1 cut(s) 373
BstC8I GCNNGC 6 cut(s) 29, 484, 584, 633, 667, 1068
BstDEI CTNAG 6 cut(s) 336, 386, 487, 532, 625, 772
BstEII GGTNACC 1 cut(s) 494
BstKTI GATC 3 cut(s) 185, 504, 923
BstMBI GATC 3 cut(s) 182, 501, 920
BstMWI GCNNNNNNNGC 2 cut(s) 373, 488
BstNI CCWGG 1 cut(s) 840
BstPI GGTNACC 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 838
BstSFI CTRYAG 1 cut(s) 752
BstSLI GKGCMC 1 cut(s) 349
BstV1I GCAGC 4 cut(s) 402, 491, 613, 835
Bsu15I ATCGAT 1 cut(s) 504
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 4 cut(s) 90, 152, 258, 1031
BsuTUI ATCGAT 1 cut(s) 504
BtgZI GCGATG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 23
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 257, 726
BveI ACCTGC 1 cut(s) 759
Cac8I GCNNGC 6 cut(s) 29, 484, 584, 633, 667, 1068
Cfr13I GGNCC 5 cut(s) 89, 151, 215, 308, 1030
ClaI ATCGAT 1 cut(s) 504
CsiI ACCWGGT 1 cut(s) 838
Csp6I GTAC 1 cut(s) 550
CviAII CATG 4 cut(s) 34, 95, 315, 368
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 6 cut(s) 336, 386, 487, 532, 625, 772
DpnI GATC 3 cut(s) 184, 503, 922
DpnII GATC 3 cut(s) 182, 501, 920
DraI TTTAAA 1 cut(s) 270
EaeI YGGCCR 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 215, 308
Eco91I GGTNACC 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 4 cut(s) 37, 98, 318, 371
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FaqI GGGAC 1 cut(s) 451
FatI CATG 4 cut(s) 33, 94, 314, 367
FauI CCCGC 1 cut(s) 369
Fnu4HI GCNGC 4 cut(s) 391, 480, 602, 849
Fsp4HI GCNGC 4 cut(s) 391, 480, 602, 849
FspBI CTAG 3 cut(s) 18, 74, 662
GluI GCNGC 4 cut(s) 391, 480, 602, 849
HaeIII GGCC 4 cut(s) 90, 152, 258, 1031
Hin1II CATG 4 cut(s) 37, 98, 318, 371
HincII GTYRAC 1 cut(s) 1077
HindII GTYRAC 1 cut(s) 1077
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 1 cut(s) 691
HphI GGTGA 1 cut(s) 949
Hpy166II GTNNAC 2 cut(s) 680, 1077
Hpy188I TCNGA 3 cut(s) 337, 535, 1044
Hpy188III TCNNGA 4 cut(s) 474, 918, 970, 1012
Hpy8I GTNNAC 2 cut(s) 680, 1077
HpyAV CCTTC 2 cut(s) 321, 1054
HpyCH4III ACNGT 2 cut(s) 721, 760
HpyCH4IV ACGT 2 cut(s) 22, 944
HpyCH4V TGCA 8 cut(s) 164, 193, 227, 250, 443, 754, 780, 962
HpyF10VI GCNNNNNNNGC 2 cut(s) 373, 488
HpyF3I CTNAG 6 cut(s) 336, 386, 487, 532, 625, 772
HpySE526I ACGT 2 cut(s) 22, 944
Hsp92II CATG 4 cut(s) 37, 98, 318, 371
Kzo9I GATC 3 cut(s) 182, 501, 920
LmnI GCTCC 4 cut(s) 112, 283, 884, 1063
Lsp1109I GCAGC 4 cut(s) 402, 491, 613, 835
LweI GCATC 3 cut(s) 62, 173, 971
MabI ACCWGGT 1 cut(s) 838
MaeI CTAG 3 cut(s) 18, 74, 662
MaeII ACGT 2 cut(s) 22, 944
MaeIII GTNAC 3 cut(s) 494, 591, 1018
MalI GATC 3 cut(s) 184, 503, 922
MboI GATC 3 cut(s) 182, 501, 920
MboII GAAGA 4 cut(s) 62, 159, 351, 1057
MfeI CAATTG 2 cut(s) 444, 605
MhlI GDGCHC 2 cut(s) 280, 349
MnlI CCTC 8 cut(s) 103, 228, 409, 468, 538, 541, 660, 875
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 2 cut(s) 269, 741
MslI CAYNNNNRTG 1 cut(s) 1025
MspR9I CCNGG 1 cut(s) 840
MunI CAATTG 2 cut(s) 444, 605
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 1 cut(s) 840
MwoI GCNNNNNNNGC 2 cut(s) 373, 488
NdeII GATC 3 cut(s) 182, 501, 920
NlaIII CATG 4 cut(s) 37, 98, 318, 371
NlaIV GGNNCC 5 cut(s) 91, 216, 346, 381, 642
NmeAIII GCCGAG 1 cut(s) 284
NmuCI GTSAC 2 cut(s) 591, 1018
PaqCI CACCTGC 1 cut(s) 759
PctI GAATGC 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 691
PkrI GCNGC 4 cut(s) 392, 481, 603, 850
PpuMI RGGWCCY 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 838
PspEI GGTNACC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 5 cut(s) 91, 216, 346, 381, 642
PspPI GGNCC 5 cut(s) 89, 151, 215, 308, 1030
PspPPI RGGWCCY 1 cut(s) 215
PstI CTGCAG 1 cut(s) 756
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 1 cut(s) 1025
SaqAI TTAA 2 cut(s) 269, 741
SatI GCNGC 4 cut(s) 391, 480, 602, 849
Sau3AI GATC 3 cut(s) 182, 501, 920
Sau96I GGNCC 5 cut(s) 89, 151, 215, 308, 1030
ScrFI CCNGG 1 cut(s) 840
SduI GDGCHC 2 cut(s) 280, 349
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 3 cut(s) 62, 173, 971
SfcI CTRYAG 1 cut(s) 752
SinI GGWCC 2 cut(s) 215, 308
SmiMI CAYNNNNRTG 1 cut(s) 1025
SmlI CTYRAG 1 cut(s) 472
SmoI CTYRAG 1 cut(s) 472
SsiI CCGC 1 cut(s) 376
SspI AATATT 1 cut(s) 125
SspMI CTAG 3 cut(s) 18, 74, 662
StyD4I CCNGG 1 cut(s) 838
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 2 cut(s) 721, 760
TaiI ACGT 2 cut(s) 25, 947
TaqI TCGA 3 cut(s) 108, 170, 504
TfiI GAWTC 1 cut(s) 691
Tru1I TTAA 2 cut(s) 269, 741
Tru9I TTAA 2 cut(s) 269, 741
TscAI CASTG 2 cut(s) 257, 726
TseFI GTSAC 2 cut(s) 591, 1018
TseI GCWGC 4 cut(s) 390, 479, 601, 848
Tsp45I GTSAC 2 cut(s) 591, 1018
TspDTI ATGAA 8 cut(s) 303, 351, 457, 522, 552, 754, 823, 825
TspRI CASTG 2 cut(s) 257, 726
VpaK11BI GGWCC 2 cut(s) 215, 308
XapI RAATTY 2 cut(s) 66, 704
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 3 cut(s) 18, 74, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.