MD04G1204700.v1.1

Vacuole membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002021
Physical Location & Seq
Forward (+)
29101149 .. 29104904
3756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1204700.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGATCCCACAGACGCAGCCTCGTCGAATTCCACGGATTCCACCATCTCCGGACTCCGTTTGAAGCATCACCGGGAACTAGAAAGGTTAACACTTACAATGCAGCCGCTAAAGACGTTAAAGTATTTCAATTTGGCTGTTGGCCAATATGTTCGGCGGTTCATGGCGAAAGGTGGTTGGTTTGTGCTTCTAATTATGTTAGCAGGAGGTATAGGAGTGATTACTATGACAATTGGTGGACTGCAACAAGAGCATGTTTGGGAGCTTATTCGTTATCTCCAATTTGGTCTCTGGTGGCTGGCTCTTGGTGTAGCATCATCAATTGGTCTTGGATCTGGTTTGCACACGTTTGTGCTGTATTTGGGGCCTCATATAGCTCTTTTTACCATGAAAGCCGTGGACTGTGGCAGAGTTGACATAAAAGGTGCCCCCTATGACACCATTCAATGGAGAAGTGGTCCTTCATGGGTGGACAGAGAATGCTCTGAATATGGCGCCCCACTATTCTTATCACAGCACGGTTCAAGGGTTCCTCTTAGCAGCATTTTGCCTCAGGTTCAGCTAGAGGCTATTCTATGGGGAGTCGGAACTGCACTGGGAGAGCTTCCTCCGTATTTCATATCAAGAGCCGCTAGTATATCTGGTCAAAAGTTGGAAGTCATGAAAGAGTTGGATTCTTCCTCAACTGAAGATAATGGAATGATAGCTAACCGCCTCAACCGGATGAAATCATGGCTTCTTTCGCATACACAATATCTGAACTTTTTAACTATATTAGTTCTTGCTTCGGTGCCCAACCCTCTATTTGACCTTGCTGGTATATTGTGTGGACAATTTGGCATTCGGTTTTGGACATTCTTTCTAGCAACATTGATTGGAAAGGCATTTATTAAAACTCACATACAGACAGTTTTCATCATCTCAGTTTGCAACAATCAGCTTCTAGACTTGGTAGAAAATAAATTGATTTGGTTGTTTGGCTTTTTCCCTGGATTTGGTTTGGTTGTGCCCGACCTCATCAGCAAACTACATACTGTGAAAGACAAGTATGTACATGCCTCACCTCCAGCCCCCTCAAACAGCAAGGATAAAAAGTGGGACTTATCATTTAGTTCGATTTGGAACACAGTTGTGTGGCTCATGCTTATGAACTTCTTCCTCAAGATTGTGACGGCAAGTGCACAAAGCGTTCTCAAGGAAGAACATGAAAGGGAGTTGACTGCATTGTCCAACAATCTGCATTCATCCAACCATAAAAGTAGTGCCGGTTGCAGCCAAGCCTCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 map04140
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

47.72

Weight (kDa)

8.69

Isoelectric Point (pI)

31.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 241 - 295 7.1e-06 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1225
AccB1I GGYRCC 3 cut(s) 425, 494, 790
AccB7I CCANNNNNTGG 1 cut(s) 447
AccIII TCCGGA 1 cut(s) 50
AciI CCGC 4 cut(s) 107, 157, 630, 712
AclWI GGATC 2 cut(s) 12, 340
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 1 cut(s) 1053
AfiI CCNNNNNNNGG 2 cut(s) 447, 995
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 4 cut(s) 64, 130, 446, 525
AjnI CCWGG 1 cut(s) 988
AleI CACNNNNGTG 2 cut(s) 350, 1130
AluBI AGCT 6 cut(s) 265, 377, 562, 604, 707, 940
AluI AGCT 6 cut(s) 265, 377, 562, 604, 707, 940
Alw21I GWGCWC 1 cut(s) 1183
Alw26I GTCTC 1 cut(s) 293
Alw44I GTGCAC 1 cut(s) 1179
AlwI GGATC 2 cut(s) 12, 340
Aor13HI TCCGGA 1 cut(s) 50
AoxI GGCC 2 cut(s) 142, 365
ApaLI GTGCAC 1 cut(s) 1179
ApeKI GCWGC 4 cut(s) 17, 103, 540, 1272
ApoI RAATTY 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 1154
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 2 cut(s) 365, 458
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 62, 1053
AvaII GGWCC 1 cut(s) 458
AxyI CCTNAGG 1 cut(s) 552
BaeGI GKGCMC 4 cut(s) 430, 795, 1011, 1183
BalI TGGCCA 1 cut(s) 144
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 425, 494, 790
Bbv12I GWGCWC 1 cut(s) 1183
BbvI GCAGC 4 cut(s) 29, 115, 552, 1284
BccI CCATC 1 cut(s) 53
BceAI ACGGC 2 cut(s) 380, 1188
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 990
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 5 cut(s) 80, 563, 633, 863, 944
BfoI RGCGCY 1 cut(s) 498
BisI GCNGC 6 cut(s) 18, 104, 107, 541, 630, 1273
BlsI GCNGC 6 cut(s) 19, 105, 108, 542, 631, 1274
Bme1390I CCNGG 2 cut(s) 74, 990
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 365, 458
BmiI GGNNCC 6 cut(s) 6, 366, 427, 496, 531, 792
BmrFI CCNGG 2 cut(s) 74, 990
BmrI ACTGGG 1 cut(s) 605
BmsI GCATC 2 cut(s) 76, 323
BmuI ACTGGG 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 1050
BpuEI CTTGAG 2 cut(s) 1145, 1178
BpuMI CCSGG 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 3 cut(s) 33, 396, 988
BsaWI WCCGGW 2 cut(s) 50, 720
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 447, 995
Bse118I RCCGGY 1 cut(s) 1265
Bse1I ACTGG 1 cut(s) 600
Bse21I CCTNAGG 1 cut(s) 552
BseAI TCCGGA 1 cut(s) 50
BseBI CCWGG 1 cut(s) 990
BseDI CCNNGG 3 cut(s) 33, 396, 988
BseGI GGATG 2 cut(s) 729, 1244
BseLI CCNNNNNNNGG 2 cut(s) 447, 995
BseMII CTCAG 2 cut(s) 566, 936
BseNI ACTGG 1 cut(s) 600
BseSI GKGCMC 4 cut(s) 430, 795, 1011, 1183
BseXI GCAGC 4 cut(s) 29, 115, 552, 1284
BsgI GTGCAG 1 cut(s) 576
BshFI GGCC 2 cut(s) 144, 367
BshNI GGYRCC 3 cut(s) 425, 494, 790
BsiHKAI GWGCWC 1 cut(s) 1183
BsiSI CCGG 4 cut(s) 51, 73, 721, 1266
BslFI GGGAC 1 cut(s) 1112
BslI CCNNNNNNNGG 2 cut(s) 447, 995
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 1112
BsmI GAATGC 3 cut(s) 485, 840, 1240
BsnI GGCC 2 cut(s) 144, 367
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 4 cut(s) 430, 795, 1011, 1183
Bsp13I TCCGGA 1 cut(s) 50
Bsp1407I TGTACA 1 cut(s) 1051
Bsp143I GATC 2 cut(s) 4, 332
BspACI CCGC 4 cut(s) 107, 157, 630, 712
BspANI GGCC 2 cut(s) 144, 367
BspCNI CTCAG 2 cut(s) 565, 935
BspEI TCCGGA 1 cut(s) 50
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 6 cut(s) 6, 366, 427, 496, 531, 792
BspPI GGATC 2 cut(s) 12, 340
BspT107I GGYRCC 3 cut(s) 425, 494, 790
BspTNI GGTCTC 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 1265
BsrGI TGTACA 1 cut(s) 1051
BsrI ACTGG 1 cut(s) 600
BssAI RCCGGY 1 cut(s) 1265
BssECI CCNNGG 3 cut(s) 33, 396, 988
BssMI GATC 2 cut(s) 4, 332
BssNI GRCGYC 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 990
Bst4CI ACNGT 5 cut(s) 404, 521, 910, 1036, 1129
BstACI GRCGYC 1 cut(s) 495
BstAUI TGTACA 1 cut(s) 1051
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 3 cut(s) 536, 552, 922
BstDSI CCRYGG 2 cut(s) 33, 396
BstF5I GGATG 2 cut(s) 729, 1244
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 2 cut(s) 7, 335
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 2 cut(s) 4, 332
BstMWI GCNNNNNNNGC 2 cut(s) 250, 742
BstNI CCWGG 1 cut(s) 990
BstNSI RCATGY 2 cut(s) 257, 1058
BstSCI CCNGG 2 cut(s) 72, 988
BstSLI GKGCMC 4 cut(s) 430, 795, 1011, 1183
BstV1I GCAGC 4 cut(s) 29, 115, 552, 1284
BstX2I RGATCY 2 cut(s) 4, 332
BstYI RGATCY 2 cut(s) 4, 332
Bsu36I CCTNAGG 1 cut(s) 552
BsuRI GGCC 2 cut(s) 144, 367
BtgI CCRYGG 2 cut(s) 33, 396
BtsCI GGATG 2 cut(s) 729, 1244
BtsIMutI CAGTG 1 cut(s) 593
Cac8I GCNNGC 1 cut(s) 300
CciI TCATGA 1 cut(s) 660
CfoI GCGC 1 cut(s) 497
Cfr10I RCCGGY 1 cut(s) 1265
Cfr13I GGNCC 2 cut(s) 365, 458
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 1052
CviAII CATG 9 cut(s) 163, 254, 388, 465, 661, 732, 1055, 1141, 1205
CviQI GTAC 1 cut(s) 1052
DdeI CTNAG 3 cut(s) 536, 552, 922
DinI GGCGCC 1 cut(s) 496
DpnI GATC 2 cut(s) 6, 334
DpnII GATC 2 cut(s) 4, 332
DrdI GACNNNNNNGTC 1 cut(s) 1225
DseDI GACNNNNNNGTC 1 cut(s) 1225
EaeI YGGCCR 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 458
Eco57I CTGAAG 1 cut(s) 708
Eco81I CCTNAGG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 365
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 988
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
FaeI CATG 9 cut(s) 166, 257, 391, 468, 664, 735, 1058, 1144, 1208
FalI AAGNNNNNCTT 4 cut(s) 445, 477, 1085, 1117
FaqI GGGAC 1 cut(s) 1112
FatI CATG 9 cut(s) 162, 253, 387, 464, 660, 731, 1054, 1140, 1204
Fnu4HI GCNGC 6 cut(s) 18, 104, 107, 541, 630, 1273
FokI GGATG 2 cut(s) 736, 1231
Fsp4HI GCNGC 6 cut(s) 18, 104, 107, 541, 630, 1273
FspBI CTAG 5 cut(s) 80, 563, 633, 863, 944
GlaI GCGC 1 cut(s) 496
GluI GCNGC 6 cut(s) 18, 104, 107, 541, 630, 1273
GsuI CTGGAG 1 cut(s) 1050
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 2 cut(s) 144, 367
HapII CCGG 4 cut(s) 51, 73, 721, 1266
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 9 cut(s) 166, 257, 391, 468, 664, 735, 1058, 1144, 1208
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HincII GTYRAC 3 cut(s) 90, 415, 1218
HindII GTYRAC 3 cut(s) 90, 415, 1218
HinfI GANTC 4 cut(s) 38, 54, 582, 674
HpaI GTTAAC 1 cut(s) 90
HpaII CCGG 4 cut(s) 51, 73, 721, 1266
HphI GGTGA 2 cut(s) 62, 1053
Hpy166II GTNNAC 8 cut(s) 90, 239, 400, 415, 472, 830, 1181, 1218
Hpy188I TCNGA 4 cut(s) 487, 587, 759, 1285
Hpy188III TCNNGA 5 cut(s) 51, 624, 661, 944, 1162
Hpy8I GTNNAC 8 cut(s) 90, 239, 400, 415, 472, 830, 1181, 1218
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 5 cut(s) 404, 521, 910, 1036, 1129
HpyCH4IV ACGT 2 cut(s) 116, 347
HpyCH4V TGCA 9 cut(s) 103, 244, 343, 593, 930, 1181, 1223, 1240, 1272
HpyF10VI GCNNNNNNNGC 2 cut(s) 250, 742
HpyF3I CTNAG 3 cut(s) 536, 552, 922
HpySE526I ACGT 2 cut(s) 116, 347
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 9 cut(s) 166, 257, 391, 468, 664, 735, 1058, 1144, 1208
HspAI GCGC 1 cut(s) 495
KasI GGCGCC 1 cut(s) 494
Kpn2I TCCGGA 1 cut(s) 50
KspAI GTTAAC 1 cut(s) 90
Kzo9I GATC 2 cut(s) 4, 332
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 4 cut(s) 29, 115, 552, 1284
LweI GCATC 2 cut(s) 76, 323
MaeI CTAG 5 cut(s) 80, 563, 633, 863, 944
MaeII ACGT 2 cut(s) 116, 347
MaeIII GTNAC 1 cut(s) 1168
MalI GATC 2 cut(s) 6, 334
MboI GATC 2 cut(s) 4, 332
MboII GAAGA 4 cut(s) 669, 701, 1147, 1211
MfeI CAATTG 2 cut(s) 231, 321
MflI RGATCY 2 cut(s) 4, 332
MhlI GDGCHC 4 cut(s) 430, 795, 1011, 1183
MlsI TGGCCA 1 cut(s) 144
MluCI AATT 8 cut(s) 28, 130, 192, 231, 281, 321, 833, 962
MluNI TGGCCA 1 cut(s) 144
Mly113I GGCGCC 1 cut(s) 495
MlyI GAGTC 2 cut(s) 48, 591
MmeI TCCRAC 5 cut(s) 565, 633, 651, 1254, 1272
Mox20I TGGCCA 1 cut(s) 144
MroI TCCGGA 1 cut(s) 50
MroXI GAANNNNTTC 1 cut(s) 1154
MscI TGGCCA 1 cut(s) 144
MseI TTAA 4 cut(s) 89, 119, 767, 891
MslI CAYNNNNRTG 3 cut(s) 350, 1130, 1145
Msp20I TGGCCA 1 cut(s) 144
MspI CCGG 4 cut(s) 51, 73, 721, 1266
MspR9I CCNGG 2 cut(s) 74, 990
MunI CAATTG 2 cut(s) 231, 321
Mva1269I GAATGC 3 cut(s) 485, 840, 1240
MvaI CCWGG 1 cut(s) 990
MwoI GCNNNNNNNGC 2 cut(s) 250, 742
NarI GGCGCC 1 cut(s) 495
NciI CCSGG 1 cut(s) 74
NdeII GATC 2 cut(s) 4, 332
NlaIII CATG 9 cut(s) 166, 257, 391, 468, 664, 735, 1058, 1144, 1208
NlaIV GGNNCC 6 cut(s) 6, 366, 427, 496, 531, 792
NmuCI GTSAC 1 cut(s) 1168
NspI RCATGY 2 cut(s) 257, 1058
OliI CACNNNNGTG 2 cut(s) 350, 1130
PagI TCATGA 1 cut(s) 660
PctI GAATGC 3 cut(s) 485, 840, 1240
PdmI GAANNNNTTC 1 cut(s) 1154
PfeI GAWTC 2 cut(s) 38, 674
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 6 cut(s) 19, 105, 108, 542, 631, 1274
PleI GAGTC 2 cut(s) 48, 590
PluTI GGCGCC 1 cut(s) 498
PpsI GAGTC 2 cut(s) 48, 590
Psp6I CCWGG 1 cut(s) 988
PspGI CCWGG 1 cut(s) 988
PspN4I GGNNCC 6 cut(s) 6, 366, 427, 496, 531, 792
PspPI GGNCC 2 cut(s) 365, 458
PsuI RGATCY 2 cut(s) 4, 332
RsaI GTAC 1 cut(s) 1053
RsaNI GTAC 1 cut(s) 1052
RseI CAYNNNNRTG 3 cut(s) 350, 1130, 1145
SaqAI TTAA 4 cut(s) 89, 119, 767, 891
SatI GCNGC 6 cut(s) 18, 104, 107, 541, 630, 1273
Sau3AI GATC 2 cut(s) 4, 332
Sau96I GGNCC 2 cut(s) 365, 458
SchI GAGTC 2 cut(s) 48, 591
ScrFI CCNGG 2 cut(s) 74, 990
SduI GDGCHC 4 cut(s) 430, 795, 1011, 1183
SfaNI GCATC 2 cut(s) 76, 323
SfoI GGCGCC 1 cut(s) 496
SinI GGWCC 1 cut(s) 458
SmiMI CAYNNNNRTG 3 cut(s) 350, 1130, 1145
SmlI CTYRAG 2 cut(s) 1160, 1193
SmoI CTYRAG 2 cut(s) 1160, 1193
Sse9I AATT 8 cut(s) 28, 130, 192, 231, 281, 321, 833, 962
SsiI CCGC 4 cut(s) 107, 157, 630, 712
SspDI GGCGCC 1 cut(s) 494
SspMI CTAG 5 cut(s) 80, 563, 633, 863, 944
StyD4I CCNGG 2 cut(s) 72, 988
TaaI ACNGT 5 cut(s) 404, 521, 910, 1036, 1129
TaiI ACGT 2 cut(s) 119, 350
TaqI TCGA 2 cut(s) 26, 1115
TasI AATT 8 cut(s) 28, 130, 192, 231, 281, 321, 833, 962
TatI WGTACW 1 cut(s) 1051
TauI GCSGC 2 cut(s) 109, 632
TfiI GAWTC 2 cut(s) 38, 674
Tru1I TTAA 4 cut(s) 89, 119, 767, 891
Tru9I TTAA 4 cut(s) 89, 119, 767, 891
TscAI CASTG 1 cut(s) 600
TseFI GTSAC 1 cut(s) 1168
TseI GCWGC 4 cut(s) 17, 103, 540, 1272
Tsp45I GTSAC 1 cut(s) 1168
TspGWI ACGGA 3 cut(s) 47, 50, 600
TspRI CASTG 1 cut(s) 600
Van91I CCANNNNNTGG 1 cut(s) 447
VneI GTGCAC 1 cut(s) 1179
VpaK11BI GGWCC 1 cut(s) 458
XapI RAATTY 1 cut(s) 28
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 2 cut(s) 257, 1058
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmnI GAANNNNTTC 1 cut(s) 1154
XspI CTAG 5 cut(s) 80, 563, 633, 863, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.