MD12G1218400.v1.1

Vacuole membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002021
Physical Location & Seq
Forward (+)
29603800 .. 29607803
4004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1218400.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGATCCCACCGACGCAGCCTCGTCGAATTCCACGGATTCCTCCATCTCCGAACTCCGTTTGAAGCATCACCAGGAACTAGAAAATTTAACGCTTGCAACGCAACCGTTAAAGACATTAAAGTATTTCAATTTGGCTATTGGCCAATATGTTCGGCGTTTTATGGCGAGAGGTGGTTGGTTTCTGCTTCTAATTATGTTAGCAGGAGGTATAGGAGTGATCACTGCGACAGTTGGTGGACCTCAACAAGAGCATATCCGGGAGCTTATTCGTTATCTGCAATTTGGACTCTGGTGGCTGGCTCTTGGTGTAGCATCATCTATTGGTCTTGGATCTGGTTTGCACACGTTTGTGCTGTATTTGGGGCCTCATGTAGCTCTGTTTACCATGAAAGCCGTGCACTGTGGCAGAGTTGACATAAAAAGTGCCCCATATGACACCATTCAGTGGAGAAGTGGTCCTTCATGGCTGGATAGAGAATGCTCTGAATATGGGACACCCCTATTGTTATCACAGCACGGTTCAAGGGTTCCGCTTAGCAGTATATTGCCTCAGGTTCAGTTAGAGGCCATTCTATGGGGAGTTGGAACTGCACTGGGAGAGCTTCCTCCTTATTTCATATCAAGAGCTGCTAGTATATCTGGTCAAAAATTGGAAGTCATGAAAGAATTGGATTCTTCATCAACTGAAGACAGTGGAATGGTAGCTAGTCGCCTCAACCGGATGAAATCATGGCTTCTTTCACATACACAATATCTGAACTTTTTAACTATATTAGTTCTTGCTTCGGTGCCCAACCCTCTATTTGACCTTGCTGGGATATTGTGTGGACAATTTGGCATTCGATTTTGGACGTTCTTTCTAGCAACATTGATTGGAAAGGCATTTATTAAGACTCACATACAGACAGTTTTCATCATCTCAGTTTGCAACAATCAGCTTCTCGACTTGGTAGAAAATAAATTGATTTGGTTGTTCAGCTTTTTCCCTGGATTTGGTTTGTTTGTGCCCAACCTCATCACCAAATTACATACAATGAAAGACAAGTATATACACGCCTCACCTCCAGTCGCCTCTAACAGCGAGGAGAAAAAGTGGGACTTATCCTTTAGTTCGATTTGGAACACTGTTGTGTGGCTCATGCTCATGAACTTTTTCATCAAGATTGTGACGGCAAGTGCACAAAGCGTTCTCAAGGAAGAACATGAAAAGGAGTTGACTGCGTTGTCGAACAATCTGCATACATCCAAACAAAAAAGTATTGCTAGTTGCAGCCAAGCCTCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 map04140
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

47.78

Weight (kDa)

8.49

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 242 - 295 6.4e-06 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1225
AccB1I GGYRCC 1 cut(s) 790
AccB7I CCANNNNNTGG 2 cut(s) 447, 576
AciI CCGC 1 cut(s) 533
AclWI GGATC 2 cut(s) 12, 340
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 2 cut(s) 28, 85
AcuI CTGAAG 1 cut(s) 708
AfiI CCNNNNNNNGG 3 cut(s) 447, 576, 995
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 3 cut(s) 64, 130, 525
AjnI CCWGG 2 cut(s) 72, 988
AleI CACNNNNGTG 2 cut(s) 350, 1130
AluBI AGCT 7 cut(s) 265, 377, 604, 629, 707, 940, 981
AluI AGCT 7 cut(s) 265, 377, 604, 629, 707, 940, 981
Alw21I GWGCWC 2 cut(s) 402, 1183
Alw44I GTGCAC 2 cut(s) 398, 1179
AlwI GGATC 2 cut(s) 12, 340
AoxI GGCC 3 cut(s) 142, 365, 567
ApaLI GTGCAC 2 cut(s) 398, 1179
ApeKI GCWGC 3 cut(s) 17, 629, 1272
ApoI RAATTY 2 cut(s) 28, 85
Asp700I GAANNNNTTC 1 cut(s) 1154
AspS9I GGNCC 3 cut(s) 239, 365, 458
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 3 cut(s) 62, 1012, 1053
AvaII GGWCC 2 cut(s) 239, 458
AxyI CCTNAGG 1 cut(s) 552
BaeGI GKGCMC 5 cut(s) 402, 430, 795, 1011, 1183
BalI TGGCCA 1 cut(s) 144
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 790
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 2 cut(s) 402, 1183
BbvI GCAGC 3 cut(s) 29, 616, 1284
BccI CCATC 1 cut(s) 53
BceAI ACGGC 2 cut(s) 380, 1188
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 1219, 1253
BciT130I CCWGG 2 cut(s) 74, 990
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 1 cut(s) 260
BfaI CTAG 5 cut(s) 80, 633, 708, 863, 1266
BisI GCNGC 3 cut(s) 18, 630, 1273
BlpI GCTNAGC 1 cut(s) 536
BlsI GCNGC 3 cut(s) 19, 631, 1274
Bme1390I CCNGG 3 cut(s) 74, 260, 990
Bme18I GGWCC 2 cut(s) 239, 458
BmgT120I GGNCC 3 cut(s) 239, 365, 458
BmiI GGNNCC 4 cut(s) 6, 366, 531, 792
BmrFI CCNGG 3 cut(s) 74, 260, 990
BmrI ACTGGG 1 cut(s) 605
BmsI GCATC 2 cut(s) 76, 323
BmuI ACTGGG 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 696
BpmI CTGGAG 1 cut(s) 1050
Bpu1102I GCTNAGC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 1178
BpuMI CCSGG 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 33, 988
BsaWI WCCGGW 1 cut(s) 720
BsaXI ACNNNNNCTCC 4 cut(s) 442, 472, 1079, 1109
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 576, 995
Bse1I ACTGG 2 cut(s) 600, 1067
Bse21I CCTNAGG 1 cut(s) 552
BseBI CCWGG 2 cut(s) 74, 990
BseDI CCNNGG 2 cut(s) 33, 988
BseGI GGATG 2 cut(s) 729, 1244
BseLI CCNNNNNNNGG 3 cut(s) 447, 576, 995
BseMII CTCAG 2 cut(s) 566, 936
BseNI ACTGG 2 cut(s) 600, 1067
BseRI GAGGAG 1 cut(s) 1100
BseSI GKGCMC 5 cut(s) 402, 430, 795, 1011, 1183
BseXI GCAGC 3 cut(s) 29, 616, 1284
BseYI CCCAGC 1 cut(s) 815
BsgI GTGCAG 1 cut(s) 576
BshFI GGCC 3 cut(s) 144, 367, 569
BshNI GGYRCC 1 cut(s) 790
BsiHKAI GWGCWC 2 cut(s) 402, 1183
BsiSI CCGG 2 cut(s) 259, 721
BslFI GGGAC 2 cut(s) 508, 1112
BslI CCNNNNNNNGG 3 cut(s) 447, 576, 995
BsmFI GGGAC 2 cut(s) 508, 1112
BsmI GAATGC 2 cut(s) 485, 840
BsnI GGCC 3 cut(s) 144, 367, 569
Bsp1286I GDGCHC 5 cut(s) 402, 430, 795, 1011, 1183
Bsp143I GATC 3 cut(s) 4, 219, 332
Bsp1720I GCTNAGC 1 cut(s) 536
BspACI CCGC 1 cut(s) 533
BspANI GGCC 3 cut(s) 144, 367, 569
BspCNI CTCAG 2 cut(s) 565, 935
BspHI TCATGA 2 cut(s) 660, 1146
BspLI GGNNCC 4 cut(s) 6, 366, 531, 792
BspPI GGATC 2 cut(s) 12, 340
BspT107I GGYRCC 1 cut(s) 790
BsrI ACTGG 2 cut(s) 600, 1067
BssECI CCNNGG 2 cut(s) 33, 988
BssMI GATC 3 cut(s) 4, 219, 332
Bst2UI CCWGG 2 cut(s) 74, 990
Bst4CI ACNGT 7 cut(s) 108, 232, 404, 521, 695, 910, 1129
BstC8I GCNNGC 2 cut(s) 96, 300
BstDEI CTNAG 3 cut(s) 536, 552, 922
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 2 cut(s) 729, 1244
BstKTI GATC 3 cut(s) 7, 222, 335
BstMBI GATC 3 cut(s) 4, 219, 332
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 2 cut(s) 74, 990
BstSCI CCNGG 3 cut(s) 72, 258, 988
BstSLI GKGCMC 5 cut(s) 402, 430, 795, 1011, 1183
BstV1I GCAGC 3 cut(s) 29, 616, 1284
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 2 cut(s) 4, 332
BstYI RGATCY 2 cut(s) 4, 332
Bsu36I CCTNAGG 1 cut(s) 552
BsuRI GGCC 3 cut(s) 144, 367, 569
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 2 cut(s) 729, 1244
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 6 cut(s) 222, 400, 452, 593, 700, 1125
Cac8I GCNNGC 2 cut(s) 96, 300
CciI TCATGA 2 cut(s) 660, 1146
Cfr13I GGNCC 3 cut(s) 239, 365, 458
CseI GACGC 1 cut(s) 23
CviAII CATG 8 cut(s) 371, 388, 465, 661, 732, 1141, 1147, 1205
DdeI CTNAG 3 cut(s) 536, 552, 922
DpnI GATC 3 cut(s) 6, 221, 334
DpnII GATC 3 cut(s) 4, 219, 332
DrdI GACNNNNNNGTC 1 cut(s) 1225
DseDI GACNNNNNNGTC 1 cut(s) 1225
EaeI YGGCCR 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 239, 458
Eco57I CTGAAG 1 cut(s) 708
Eco81I CCTNAGG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 365
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 72, 988
FaeI CATG 8 cut(s) 374, 391, 468, 664, 735, 1144, 1150, 1208
FalI AAGNNNNNCTT 4 cut(s) 445, 477, 1085, 1117
FaqI GGGAC 2 cut(s) 508, 1112
FatI CATG 8 cut(s) 370, 387, 464, 660, 731, 1140, 1146, 1204
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 18, 630, 1273
FokI GGATG 2 cut(s) 736, 1231
Fsp4HI GCNGC 3 cut(s) 18, 630, 1273
FspBI CTAG 5 cut(s) 80, 633, 708, 863, 1266
GluI GCNGC 3 cut(s) 18, 630, 1273
GsaI CCCAGC 1 cut(s) 819
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 3 cut(s) 144, 367, 569
HapII CCGG 2 cut(s) 259, 721
HgaI GACGC 1 cut(s) 23
Hin1II CATG 8 cut(s) 374, 391, 468, 664, 735, 1144, 1150, 1208
HincII GTYRAC 2 cut(s) 415, 1218
HindII GTYRAC 2 cut(s) 415, 1218
HinfI GANTC 4 cut(s) 38, 288, 674, 895
HpaII CCGG 2 cut(s) 259, 721
HphI GGTGA 3 cut(s) 62, 1012, 1053
Hpy166II GTNNAC 7 cut(s) 239, 384, 400, 415, 830, 1181, 1218
Hpy188I TCNGA 4 cut(s) 52, 487, 759, 1285
Hpy188III TCNNGA 5 cut(s) 624, 661, 944, 1147, 1162
Hpy8I GTNNAC 7 cut(s) 239, 384, 400, 415, 830, 1181, 1218
Hpy99I CGWCG 2 cut(s) 17, 28
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 7 cut(s) 108, 232, 404, 521, 695, 910, 1129
HpyCH4IV ACGT 2 cut(s) 347, 854
HpyCH4V TGCA 9 cut(s) 98, 280, 343, 400, 593, 930, 1181, 1240, 1272
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 3 cut(s) 536, 552, 922
HpySE526I ACGT 2 cut(s) 347, 854
Hsp92II CATG 8 cut(s) 374, 391, 468, 664, 735, 1144, 1150, 1208
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 3 cut(s) 4, 219, 332
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 3 cut(s) 29, 616, 1284
LweI GCATC 2 cut(s) 76, 323
MaeI CTAG 5 cut(s) 80, 633, 708, 863, 1266
MaeII ACGT 2 cut(s) 347, 854
MaeIII GTNAC 1 cut(s) 1168
MalI GATC 3 cut(s) 6, 221, 334
MboI GATC 3 cut(s) 4, 219, 332
MboII GAAGA 3 cut(s) 669, 701, 1211
MflI RGATCY 2 cut(s) 4, 332
MhlI GDGCHC 5 cut(s) 402, 430, 795, 1011, 1183
MlsI TGGCCA 1 cut(s) 144
MluNI TGGCCA 1 cut(s) 144
MlyI GAGTC 2 cut(s) 282, 889
MmeI TCCRAC 1 cut(s) 565
Mox20I TGGCCA 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 1154
MscI TGGCCA 1 cut(s) 144
MseI TTAA 5 cut(s) 89, 110, 119, 767, 891
MslI CAYNNNNRTG 3 cut(s) 350, 1130, 1145
Msp20I TGGCCA 1 cut(s) 144
MspI CCGG 2 cut(s) 259, 721
MspR9I CCNGG 3 cut(s) 74, 260, 990
Mva1269I GAATGC 2 cut(s) 485, 840
MvaI CCWGG 2 cut(s) 74, 990
MwoI GCNNNNNNNGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 260
NdeI CATATG 1 cut(s) 433
NdeII GATC 3 cut(s) 4, 219, 332
NlaIII CATG 8 cut(s) 374, 391, 468, 664, 735, 1144, 1150, 1208
NlaIV GGNNCC 4 cut(s) 6, 366, 531, 792
NmuCI GTSAC 1 cut(s) 1168
OliI CACNNNNGTG 2 cut(s) 350, 1130
PagI TCATGA 2 cut(s) 660, 1146
PctI GAATGC 2 cut(s) 485, 840
PdmI GAANNNNTTC 1 cut(s) 1154
PfeI GAWTC 2 cut(s) 38, 674
PflMI CCANNNNNTGG 2 cut(s) 447, 576
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 3 cut(s) 19, 631, 1274
PleI GAGTC 2 cut(s) 282, 889
PpsI GAGTC 2 cut(s) 282, 889
Psp6I CCWGG 2 cut(s) 72, 988
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 2 cut(s) 72, 988
PspN4I GGNNCC 4 cut(s) 6, 366, 531, 792
PspPI GGNCC 3 cut(s) 239, 365, 458
PsuI RGATCY 2 cut(s) 4, 332
RseI CAYNNNNRTG 3 cut(s) 350, 1130, 1145
SaqAI TTAA 5 cut(s) 89, 110, 119, 767, 891
SatI GCNGC 3 cut(s) 18, 630, 1273
Sau3AI GATC 3 cut(s) 4, 219, 332
Sau96I GGNCC 3 cut(s) 239, 365, 458
SchI GAGTC 2 cut(s) 282, 889
ScrFI CCNGG 3 cut(s) 74, 260, 990
SduI GDGCHC 5 cut(s) 402, 430, 795, 1011, 1183
SfaNI GCATC 2 cut(s) 76, 323
SinI GGWCC 2 cut(s) 239, 458
SmiMI CAYNNNNRTG 3 cut(s) 350, 1130, 1145
SmlI CTYRAG 1 cut(s) 1193
SmoI CTYRAG 1 cut(s) 1193
SsiI CCGC 1 cut(s) 533
SspMI CTAG 5 cut(s) 80, 633, 708, 863, 1266
StyD4I CCNGG 3 cut(s) 72, 258, 988
TaaI ACNGT 7 cut(s) 108, 232, 404, 521, 695, 910, 1129
TaiI ACGT 2 cut(s) 350, 857
TaqI TCGA 5 cut(s) 26, 844, 945, 1115, 1229
TfiI GAWTC 2 cut(s) 38, 674
Tru1I TTAA 5 cut(s) 89, 110, 119, 767, 891
Tru9I TTAA 5 cut(s) 89, 110, 119, 767, 891
TscAI CASTG 6 cut(s) 229, 407, 452, 600, 700, 1132
TseFI GTSAC 1 cut(s) 1168
TseI GCWGC 3 cut(s) 17, 629, 1272
Tsp45I GTSAC 1 cut(s) 1168
TspGWI ACGGA 2 cut(s) 47, 50
TspRI CASTG 6 cut(s) 229, 407, 452, 600, 700, 1132
Van91I CCANNNNNTGG 2 cut(s) 447, 576
VneI GTGCAC 2 cut(s) 398, 1179
VpaK11BI GGWCC 2 cut(s) 239, 458
XapI RAATTY 2 cut(s) 28, 85
XmnI GAANNNNTTC 1 cut(s) 1154
XspI CTAG 5 cut(s) 80, 633, 708, 863, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.