Rh4DG202700

Vacuole membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002021
Physical Location & Seq
Forward (+)
39424863 .. 39429900
5038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG202700.1

Sequence Viewer

Length: 996 bp
ATGTTTCGGCAGCATGTCGAGGAGCTTTTCAAATATTTCCAATTTGGGTTATGGTGGGTGGCCCTTGGAGTTGCATCTTCAATTGGTCTTGGATCTGGTTTGCACACTTTTGTCCTATATCTGGGTCCTCATATTGCGTTCTTTACTATAAAAGCTATGCAGTGTGGCCGAGTTGATTTAAAAAGTGCTCCTTATGATACAATACAGCTAAACAGTGGTCCTTCGTGGCTTGACAAAAACTGCTCTGAGTTTGGAGCCCCATTGTTTTCTTCATCACATGGTGTGCGGGTTCCACTAAGCAGCATATTACCACAGGTCCAGTTAGAAGCTGTTTTATGGGGTATTGGCACTGCAATTGGTGAACTTCCTCCATATTTCATCTCAAGAGCAGCAAGCTTAGCTGGTAACAAGATGGATGCAATGGAAGAATTGGATACCTCCTCAGATGAAGTACATGGGTTCATGGCTACTCGCCTATACCAAATTAAACGCTGGCTTCTATCACACTCACAGCAATTGAATTTCTTTACAATCTTAGTGCTTGCTTCGGTGCCAAATCCTCTTTTTGACCTAGCAGGCATTATGTGTGGACAATTTGGGGTTCCATTTTGGAAATTTTTTCTGGCAACAGTGATTGGAAAGGCTATTATTAAAACTCACCTGCAGACAGTTATCATCATCTCAGTTTGCAACAATCAACTTCTAAACTGGATAGAGAATGAATTGATTTGGGTATTCAGCTTCATACCTGGTTTTGCCGCTGTCCTGCCCAACCTCGTTGCTAAAATCGATGCAGCAAAAGAAAAGTATTTGACAGCTTCGTACTCCCCATCTCCAAATATCAAGGTGAAAACGTGGGATTTCTCATTTGCATCAATCTGGAACACTTTGGTATGGCTTATGCTATTGAACTTCTTTGTCAAGATTGTTACTTCAACAGCCCAGAGTTATCGGAAGAAAGAGCAGGAAATGGAGCTTGCTTTGTTGACAAGTTAG

Protein Analysis

331

Amino Acids

37.09

Weight (kDa)

7.69

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 115 - 216 1.2e-07 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 669
AccB1I GGYRCC 1 cut(s) 550
AciI CCGC 2 cut(s) 286, 759
AclWI GGATC 1 cut(s) 100
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 2 cut(s) 520, 614
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 2 cut(s) 453, 824
AfiI CCNNNNNNNGG 1 cut(s) 121
AgsI TTSAA 5 cut(s) 31, 81, 520, 910, 936
AjnI CCWGG 1 cut(s) 748
AluBI AGCT 9 cut(s) 25, 155, 208, 329, 396, 401, 741, 818, 976
AluI AGCT 9 cut(s) 25, 155, 208, 329, 396, 401, 741, 818, 976
Alw21I GWGCWC 1 cut(s) 190
AlwI GGATC 1 cut(s) 100
AoxI GGCC 2 cut(s) 60, 166
ApeKI GCWGC 4 cut(s) 10, 300, 389, 794
ApoI RAATTY 2 cut(s) 520, 614
AspS9I GGNCC 4 cut(s) 61, 125, 218, 316
AsuHPI GGTGA 3 cut(s) 371, 650, 859
AvaII GGWCC 3 cut(s) 125, 218, 316
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 259
Bbv12I GWGCWC 1 cut(s) 190
BbvI GCAGC 4 cut(s) 22, 312, 401, 806
BccI CCATC 2 cut(s) 406, 838
BciT130I CCWGG 1 cut(s) 750
BciVI GTATCC 1 cut(s) 427
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 1 cut(s) 662
BfuAI ACCTGC 1 cut(s) 669
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 5 cut(s) 11, 301, 390, 759, 795
BlpI GCTNAGC 1 cut(s) 397
BlsI GCNGC 5 cut(s) 12, 302, 391, 760, 796
Bme1390I CCNGG 1 cut(s) 750
Bme18I GGWCC 3 cut(s) 125, 218, 316
BmgT120I GGNCC 4 cut(s) 61, 125, 218, 316
BmiI GGNNCC 5 cut(s) 126, 256, 291, 552, 603
BmrFI CCNGG 1 cut(s) 750
BmsI GCATC 4 cut(s) 83, 406, 781, 881
Bpu1102I GCTNAGC 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 367
Bsa29I ATCGAT 1 cut(s) 789
BsaJI CCNNGG 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse1I ACTGG 2 cut(s) 319, 713
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 750
BseCI ATCGAT 1 cut(s) 789
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 3 cut(s) 237, 456, 696
BseNI ACTGG 2 cut(s) 319, 713
BseRI GAGGAG 2 cut(s) 35, 430
BseXI GCAGC 4 cut(s) 22, 312, 401, 806
BshFI GGCC 2 cut(s) 62, 168
BshNI GGYRCC 1 cut(s) 550
BshVI ATCGAT 1 cut(s) 789
BsiHKAI GWGCWC 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 121
BsnI GGCC 2 cut(s) 62, 168
Bsp1286I GDGCHC 2 cut(s) 190, 259
Bsp143I GATC 1 cut(s) 92
Bsp1720I GCTNAGC 1 cut(s) 397
BspACI CCGC 2 cut(s) 286, 759
BspANI GGCC 2 cut(s) 62, 168
BspCNI CTCAG 3 cut(s) 238, 455, 695
BspDI ATCGAT 1 cut(s) 789
BspLI GGNNCC 5 cut(s) 126, 256, 291, 552, 603
BspMAI CTGCAG 1 cut(s) 666
BspMI ACCTGC 1 cut(s) 669
BspPI GGATC 1 cut(s) 100
BspT107I GGYRCC 1 cut(s) 550
BsrDI GCAATG 1 cut(s) 426
BsrI ACTGG 2 cut(s) 319, 713
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 3 cut(s) 215, 631, 670
BstC8I GCNNGC 5 cut(s) 394, 494, 543, 577, 978
BstDEI CTNAG 6 cut(s) 246, 296, 397, 442, 535, 682
BstF5I GGATG 1 cut(s) 421
BstKTI GATC 1 cut(s) 95
BstMBI GATC 1 cut(s) 92
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNI CCWGG 1 cut(s) 750
BstNSI RCATGY 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 748
BstSFI CTRYAG 1 cut(s) 662
BstV1I GCAGC 4 cut(s) 22, 312, 401, 806
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
Bsu15I ATCGAT 1 cut(s) 789
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 2 cut(s) 62, 168
BsuTUI ATCGAT 1 cut(s) 789
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 2 cut(s) 167, 348
BtsIMutI CAGTG 4 cut(s) 167, 220, 348, 636
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 5 cut(s) 394, 494, 543, 577, 978
Cfr13I GGNCC 4 cut(s) 61, 125, 218, 316
ClaI ATCGAT 1 cut(s) 789
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 2 cut(s) 452, 823
CviAII CATG 4 cut(s) 14, 278, 455, 463
CviQI GTAC 2 cut(s) 452, 823
DdeI CTNAG 6 cut(s) 246, 296, 397, 442, 535, 682
DpnI GATC 1 cut(s) 94
DpnII GATC 1 cut(s) 92
DraI TTTAAA 1 cut(s) 180
DraIII CACNNNGTG 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 259
Eco47I GGWCC 3 cut(s) 125, 218, 316
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 4 cut(s) 17, 281, 458, 466
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FatI CATG 4 cut(s) 13, 277, 454, 462
FauI CCCGC 1 cut(s) 279
Fnu4HI GCNGC 5 cut(s) 11, 301, 390, 759, 795
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 5 cut(s) 11, 301, 390, 759, 795
FspBI CTAG 1 cut(s) 572
GluI GCNGC 5 cut(s) 11, 301, 390, 759, 795
HaeIII GGCC 2 cut(s) 62, 168
Hin1II CATG 4 cut(s) 17, 281, 458, 466
HincII GTYRAC 1 cut(s) 987
HindII GTYRAC 1 cut(s) 987
HindIII AAGCTT 1 cut(s) 394
HphI GGTGA 3 cut(s) 371, 650, 859
Hpy166II GTNNAC 3 cut(s) 362, 590, 987
Hpy188I TCNGA 3 cut(s) 247, 445, 954
Hpy188III TCNNGA 3 cut(s) 384, 880, 922
Hpy8I GTNNAC 3 cut(s) 362, 590, 987
HpyAV CCTTC 1 cut(s) 231
HpyCH4III ACNGT 3 cut(s) 215, 631, 670
HpyCH4IV ACGT 1 cut(s) 854
HpyCH4V TGCA 9 cut(s) 74, 103, 160, 353, 419, 664, 690, 794, 872
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 6 cut(s) 246, 296, 397, 442, 535, 682
HpySE526I ACGT 1 cut(s) 854
Hsp92II CATG 4 cut(s) 17, 281, 458, 466
Kzo9I GATC 1 cut(s) 92
LmnI GCTCC 4 cut(s) 22, 193, 254, 973
Lsp1109I GCAGC 4 cut(s) 22, 312, 401, 806
LweI GCATC 4 cut(s) 83, 406, 781, 881
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 1 cut(s) 572
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 2 cut(s) 404, 928
MalI GATC 1 cut(s) 94
MboI GATC 1 cut(s) 92
MboII GAAGA 4 cut(s) 69, 261, 437, 967
MfeI CAATTG 3 cut(s) 81, 354, 515
MflI RGATCY 1 cut(s) 92
MhlI GDGCHC 2 cut(s) 190, 259
MnlI CCTC 7 cut(s) 13, 138, 378, 448, 451, 570, 785
MseI TTAA 3 cut(s) 179, 486, 651
MspA1I CMGCKG 1 cut(s) 761
MspR9I CCNGG 1 cut(s) 750
MunI CAATTG 3 cut(s) 81, 354, 515
MvaI CCWGG 1 cut(s) 750
MwoI GCNNNNNNNGC 1 cut(s) 398
NdeII GATC 1 cut(s) 92
NlaIII CATG 4 cut(s) 17, 281, 458, 466
NlaIV GGNNCC 5 cut(s) 126, 256, 291, 552, 603
NmeAIII GCCGAG 1 cut(s) 194
NspI RCATGY 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 669
PkrI GCNGC 5 cut(s) 12, 302, 391, 760, 796
PpuMI RGGWCCY 1 cut(s) 125
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 748
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 5 cut(s) 126, 256, 291, 552, 603
PspPI GGNCC 4 cut(s) 61, 125, 218, 316
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 1 cut(s) 666
PsuI RGATCY 1 cut(s) 92
RsaI GTAC 2 cut(s) 453, 824
RsaNI GTAC 2 cut(s) 452, 823
SaqAI TTAA 3 cut(s) 179, 486, 651
SatI GCNGC 5 cut(s) 11, 301, 390, 759, 795
Sau3AI GATC 1 cut(s) 92
Sau96I GGNCC 4 cut(s) 61, 125, 218, 316
ScrFI CCNGG 1 cut(s) 750
SduI GDGCHC 2 cut(s) 190, 259
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 4 cut(s) 83, 406, 781, 881
SfcI CTRYAG 1 cut(s) 662
SinI GGWCC 3 cut(s) 125, 218, 316
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
SsiI CCGC 2 cut(s) 286, 759
SspI AATATT 1 cut(s) 35
SspMI CTAG 1 cut(s) 572
StyD4I CCNGG 1 cut(s) 748
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 215, 631, 670
TaiI ACGT 1 cut(s) 857
TaqI TCGA 2 cut(s) 18, 789
TatI WGTACW 1 cut(s) 451
TauI GCSGC 1 cut(s) 761
Tru1I TTAA 3 cut(s) 179, 486, 651
Tru9I TTAA 3 cut(s) 179, 486, 651
TscAI CASTG 4 cut(s) 167, 220, 355, 636
TseI GCWGC 4 cut(s) 10, 300, 389, 794
TspDTI ATGAA 6 cut(s) 261, 367, 451, 462, 733, 735
TspRI CASTG 4 cut(s) 167, 220, 355, 636
VpaK11BI GGWCC 3 cut(s) 125, 218, 316
XapI RAATTY 2 cut(s) 520, 614
XceI RCATGY 1 cut(s) 17
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.