Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0004612

Physical Location & Seq

Genomic Coordinates Forward (+)

21972673 .. 21974193

Gene Structure 1521 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g18001.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 333 bp

ATGGCAACCTCCTCATTCTCCTCACTACTACTCTGCTTCCTCTTCATCTGTTCAATCCTGGCCGCCCATTCCACGGCTTCCTCCTCCGCCGTCGATTTCGGCTGGGGCTCCGGCGGGCTTATAGGAGACTTGGATGAGGAGCTGGAGCTGCAGATGGACTCGGAGATCAGCCGGCGCATCTTAGCCACCAGCAAGTACATTAGCTACGGTGCGCTCAGGAGGAACAGTGTCCCTTGCTCGCGGCGCGGCGCGTCGTACTACAATTGCCGGCCCGGAGCTCAGGCCAATCCTTACTCCCGCGGCTGTAGCAAAATCACTCGCTGCAGGCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

111

Amino Acids

11.97

Weight (kDa)

8.86

Isoelectric Point (pI)

54.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	49 - 109	5.6e-30	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004612)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15800
fragaria_vesca	FvH4_4g18001 FvH4_6g06520
malus_domestica	MD03G1171400.v1.1 MD04G1201100.v1.1 MD12G1213600.v1.1 MD13G1168800.v1.1 MD16G1169200.v1.1
prunus_persica	Prupe.1G137000_v2.0.a1 Prupe.6G321200_v2.0.a1
pyrus_communis	pycom12g19950 pycom13g14440 pycom16g14230 pycom16g14250
rosa_chinensis	RchiOBHm_Chr3g0455671 RchiOBHm_Chr4g0422211
rosa_multiflora	Rmu_sc0002883.1_g000009 Rmu_sc0014362.1_g000002 Rmu_sc0026117.1_g000002
rosa_roxburghii	Rroxscaffold_5G00364330 Rroxscaffold_6G00423610 Rroxscaffold_6G00424110
rosa_rugosa	Rorug03G0009000 Rorug04G0178800
rosa_samantha	Rh3AG068700 Rh3BG071200 Rh3CG069500 Rh3DG071100 Rh4CG252800
rosa_wichuraiana	Rw3G005510 Rw4G020570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	4 cut(s)	241, 246, 251, 300
AciI	CCGC	7 cut(s)	63, 87, 114, 241, 246, 298, 300
AcoI	YGGCCR	1 cut(s)	60
AfaI	GTAC	2 cut(s)	197, 257
AfiI	CCNNNNNNNGG	1 cut(s)	73
AgsI	TTSAA	1 cut(s)	54
AjnI	CCWGG	1 cut(s)	57
AluBI	AGCT	4 cut(s)	142, 148, 204, 278
AluI	AGCT	4 cut(s)	142, 148, 204, 278
Alw21I	GWGCWC	1 cut(s)	280
Alw26I	GTCTC	1 cut(s)	120
AoxI	GGCC	3 cut(s)	60, 269, 282
ApeKI	GCWGC	2 cut(s)	148, 321
AspLEI	GCGC	4 cut(s)	177, 214, 246, 251
AspS9I	GGNCC	1 cut(s)	270
AsuC2I	CCSGG	1 cut(s)	273
BanII	GRGCYC	2 cut(s)	110, 280
Bbv12I	GWGCWC	1 cut(s)	280
BbvI	GCAGC	2 cut(s)	135, 308
BccI	CCATC	1 cut(s)	148
BceAI	ACGGC	2 cut(s)	74, 90
BciT130I	CCWGG	1 cut(s)	59
BcnI	CCSGG	1 cut(s)	273
BcoDI	GTCTC	1 cut(s)	120
BfmI	CTRYAG	3 cut(s)	149, 304, 322
BisI	GCNGC	6 cut(s)	63, 149, 242, 247, 301, 322
BlsI	GCNGC	6 cut(s)	64, 150, 243, 248, 302, 323
Bme1390I	CCNGG	2 cut(s)	59, 273
BmgT120I	GGNCC	1 cut(s)	270
BmiI	GGNNCC	1 cut(s)	109
BmrFI	CCNGG	2 cut(s)	59, 273
BmsI	GCATC	1 cut(s)	186
BpmI	CTGGAG	1 cut(s)	164
Bpu10I	CCTNAGC	2 cut(s)	215, 279
BpuMI	CCSGG	1 cut(s)	273
BsaJI	CCNNGG	2 cut(s)	72, 298
BsaXI	ACNNNNNCTCC	2 cut(s)	211, 241
Bsc4I	CCNNNNNNNGG	1 cut(s)	73
Bse118I	RCCGGY	2 cut(s)	171, 267
BseBI	CCWGG	1 cut(s)	59
BseDI	CCNNGG	2 cut(s)	72, 298
BseGI	GGATG	1 cut(s)	139
BseLI	CCNNNNNNNGG	1 cut(s)	73
BseMII	CTCAG	2 cut(s)	229, 293
BseRI	GAGGAG	3 cut(s)	10, 73, 152
BseXI	GCAGC	2 cut(s)	135, 308
BseYI	CCCAGC	1 cut(s)	102
Bsh1236I	CGCG	4 cut(s)	241, 246, 251, 300
BshFI	GGCC	3 cut(s)	62, 271, 284
BsiHKAI	GWGCWC	1 cut(s)	280
BsiSI	CCGG	4 cut(s)	111, 172, 268, 273
BslFI	GGGAC	1 cut(s)	215
BslI	CCNNNNNNNGG	1 cut(s)	73
BsmAI	GTCTC	1 cut(s)	120
BsmFI	GGGAC	1 cut(s)	215
BsnI	GGCC	3 cut(s)	62, 271, 284
Bsp1286I	GDGCHC	2 cut(s)	110, 280
Bsp143I	GATC	1 cut(s)	165
BspACI	CCGC	7 cut(s)	63, 87, 114, 241, 246, 298, 300
BspANI	GGCC	3 cut(s)	62, 271, 284
BspCNI	CTCAG	2 cut(s)	228, 292
BspFNI	CGCG	4 cut(s)	241, 246, 251, 300
BspLI	GGNNCC	1 cut(s)	109
BspMAI	CTGCAG	2 cut(s)	153, 326
BsrFI	RCCGGY	2 cut(s)	171, 267
BssAI	RCCGGY	2 cut(s)	171, 267
BssECI	CCNNGG	2 cut(s)	72, 298
BssMI	GATC	1 cut(s)	165
Bst2UI	CCWGG	1 cut(s)	59
Bst4CI	ACNGT	2 cut(s)	209, 227
Bst6I	CTCTTC	1 cut(s)	47
BstC8I	GCNNGC	5 cut(s)	116, 173, 239, 269, 326
BstDEI	CTNAG	3 cut(s)	181, 215, 279
BstDSI	CCRYGG	2 cut(s)	72, 298
BstF5I	GGATG	1 cut(s)	139
BstFNI	CGCG	4 cut(s)	241, 246, 251, 300
BstHHI	GCGC	4 cut(s)	177, 214, 246, 251
BstKTI	GATC	1 cut(s)	168
BstMAI	GTCTC	1 cut(s)	120
BstMBI	GATC	1 cut(s)	165
BstMWI	GCNNNNNNNGC	3 cut(s)	148, 243, 306
BstNI	CCWGG	1 cut(s)	59
BstSCI	CCNGG	2 cut(s)	57, 271
BstSFI	CTRYAG	3 cut(s)	149, 304, 322
BstUI	CGCG	4 cut(s)	241, 246, 251, 300
BstV1I	GCAGC	2 cut(s)	135, 308
BsuRI	GGCC	3 cut(s)	62, 271, 284
BtgI	CCRYGG	2 cut(s)	72, 298
BtsCI	GGATG	1 cut(s)	139
BtsIMutI	CAGTG	1 cut(s)	232
Cac8I	GCNNGC	5 cut(s)	116, 173, 239, 269, 326
CfoI	GCGC	4 cut(s)	177, 214, 246, 251
Cfr10I	RCCGGY	2 cut(s)	171, 267
Cfr13I	GGNCC	1 cut(s)	270
Cfr42I	CCGCGG	1 cut(s)	301
CseI	GACGC	1 cut(s)	240
Csp6I	GTAC	2 cut(s)	196, 256
CviQI	GTAC	2 cut(s)	196, 256
DdeI	CTNAG	3 cut(s)	181, 215, 279
DpnI	GATC	1 cut(s)	167
DpnII	GATC	1 cut(s)	165
EaeI	YGGCCR	1 cut(s)	60
Eam1104I	CTCTTC	1 cut(s)	47
EarI	CTCTTC	1 cut(s)	47
EciI	GGCGGA	1 cut(s)	76
Ecl136II	GAGCTC	1 cut(s)	278
Eco24I	GRGCYC	2 cut(s)	110, 280
Eco53kI	GAGCTC	1 cut(s)	278
EcoICRI	GAGCTC	1 cut(s)	278
EcoRII	CCWGG	1 cut(s)	57
EcoT38I	GRGCYC	2 cut(s)	110, 280
FaiI	YATR	1 cut(s)	122
FaqI	GGGAC	1 cut(s)	215
FauI	CCCGC	2 cut(s)	107, 305
Fnu4HI	GCNGC	6 cut(s)	63, 149, 242, 247, 301, 322
FokI	GGATG	1 cut(s)	146
FriOI	GRGCYC	2 cut(s)	110, 280
Fsp4HI	GCNGC	6 cut(s)	63, 149, 242, 247, 301, 322
GlaI	GCGC	4 cut(s)	176, 213, 245, 250
GluI	GCNGC	6 cut(s)	63, 149, 242, 247, 301, 322
GsaI	CCCAGC	1 cut(s)	106
GsuI	CTGGAG	1 cut(s)	164
HaeIII	GGCC	3 cut(s)	62, 271, 284
HapII	CCGG	4 cut(s)	111, 172, 268, 273
HgaI	GACGC	1 cut(s)	240
HhaI	GCGC	4 cut(s)	177, 214, 246, 251
Hin6I	GCGC	4 cut(s)	175, 212, 244, 249
HinP1I	GCGC	4 cut(s)	175, 212, 244, 249
HinfI	GANTC	1 cut(s)	158
HpaII	CCGG	4 cut(s)	111, 172, 268, 273
Hpy188I	TCNGA	1 cut(s)	163
Hpy188III	TCNNGA	1 cut(s)	217
Hpy99I	CGWCG	2 cut(s)	95, 256
HpyCH4III	ACNGT	2 cut(s)	209, 227
HpyCH4V	TGCA	2 cut(s)	151, 324
HpyF10VI	GCNNNNNNNGC	3 cut(s)	148, 243, 306
HpyF3I	CTNAG	3 cut(s)	181, 215, 279
HspAI	GCGC	4 cut(s)	175, 212, 244, 249
KroI	GCCGGC	2 cut(s)	171, 267
KroNI	GCCGGC	2 cut(s)	173, 269
KspI	CCGCGG	1 cut(s)	301
Kzo9I	GATC	1 cut(s)	165
LmnI	GCTCC	4 cut(s)	113, 139, 145, 275
Lsp1109I	GCAGC	2 cut(s)	135, 308
LweI	GCATC	1 cut(s)	186
MalI	GATC	1 cut(s)	167
MboI	GATC	1 cut(s)	165
MboII	GAAGA	1 cut(s)	34
MfeI	CAATTG	1 cut(s)	262
MhlI	GDGCHC	2 cut(s)	110, 280
MluCI	AATT	1 cut(s)	262
MlyI	GAGTC	1 cut(s)	152
MnlI	CCTC	8 cut(s)	19, 22, 31, 50, 91, 94, 130, 213
MroNI	GCCGGC	2 cut(s)	171, 267
MspA1I	CMGCKG	1 cut(s)	300
MspI	CCGG	4 cut(s)	111, 172, 268, 273
MspR9I	CCNGG	2 cut(s)	59, 273
MteI	GCGCNGCGC	1 cut(s)	247
MunI	CAATTG	1 cut(s)	262
MvaI	CCWGG	1 cut(s)	59
MvnI	CGCG	4 cut(s)	241, 246, 251, 300
MwoI	GCNNNNNNNGC	3 cut(s)	148, 243, 306
NaeI	GCCGGC	2 cut(s)	173, 269
NciI	CCSGG	1 cut(s)	273
NdeII	GATC	1 cut(s)	165
NgoMIV	GCCGGC	2 cut(s)	171, 267
NlaIV	GGNNCC	1 cut(s)	109
PdiI	GCCGGC	2 cut(s)	173, 269
PkrI	GCNGC	6 cut(s)	64, 150, 243, 248, 302, 323
PleI	GAGTC	1 cut(s)	152
PpsI	GAGTC	1 cut(s)	152
Psp124BI	GAGCTC	1 cut(s)	280
Psp6I	CCWGG	1 cut(s)	57
PspFI	CCCAGC	1 cut(s)	102
PspGI	CCWGG	1 cut(s)	57
PspN4I	GGNNCC	1 cut(s)	109
PspPI	GGNCC	1 cut(s)	270
PstI	CTGCAG	2 cut(s)	153, 326
RsaI	GTAC	2 cut(s)	197, 257
RsaNI	GTAC	2 cut(s)	196, 256
SacI	GAGCTC	1 cut(s)	280
SacII	CCGCGG	1 cut(s)	301
SatI	GCNGC	6 cut(s)	63, 149, 242, 247, 301, 322
Sau3AI	GATC	1 cut(s)	165
Sau96I	GGNCC	1 cut(s)	270
SchI	GAGTC	1 cut(s)	152
ScrFI	CCNGG	2 cut(s)	59, 273
SduI	GDGCHC	2 cut(s)	110, 280
SetI	ASST	5 cut(s)	11, 144, 150, 206, 280
SfaNI	GCATC	1 cut(s)	186
SfcI	CTRYAG	3 cut(s)	149, 304, 322
Sfr303I	CCGCGG	1 cut(s)	301
SgrBI	CCGCGG	1 cut(s)	301
Sse9I	AATT	1 cut(s)	262
SsiI	CCGC	7 cut(s)	63, 87, 114, 241, 246, 298, 300
SstI	GAGCTC	1 cut(s)	280
StyD4I	CCNGG	2 cut(s)	57, 271
TaaI	ACNGT	2 cut(s)	209, 227
TaqI	TCGA	1 cut(s)	93
TasI	AATT	1 cut(s)	262
TatI	WGTACW	1 cut(s)	195
TauI	GCSGC	4 cut(s)	65, 244, 249, 303
TscAI	CASTG	1 cut(s)	232
TseI	GCWGC	2 cut(s)	148, 321
TspDTI	ATGAA	1 cut(s)	34
TspRI	CASTG	1 cut(s)	232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.