MD12G1213600.v1.1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004612
Physical Location & Seq
Reverse (-)
29191352 .. 29191909
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1213600.v1.1.491

Sequence Viewer

Length: 387 bp
ATGGCTAGTCTGCACAGTTTCTCTCTCCTCCTCCTGATCTGCGCCTCAATATTGGCGGTGGGTTCGTCAAGTGAGGACCACCACCACCTCTCCTGGATACCCGCCGCCGCCTCCAAGTCAGCCTGCAAGGGCTCCATAGCCGACTGCGCCTTAGCTGCAGGGAACGATGAGGTGGAGTTTGACATGGACTCAGAGATCAGCCGTCGCATCTTAGCCACCAGCAACTACATCAGCTACGGTGCGCTGCAGAGGAACACCGTGCCTTGCTCCAGGCGCGGCGCCTCCTACTACAATTGCCAGCCCGGGGCCCAGTCCAACCCCTACAGCCGCGGCTGCAGCGCCATCACGAGGTGCAGGAATTTTGGCTATCGAATGAACCCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

129

Amino Acids

13.85

Weight (kDa)

8.19

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 59 - 119 2.6e-29 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AccII CGCG 2 cut(s) 276, 330
AciI CCGC 7 cut(s) 56, 102, 105, 108, 276, 328, 330
AcsI RAATTY 1 cut(s) 358
AcyI GRCGYC 1 cut(s) 279
AdeI CACNNNGTG 1 cut(s) 351
AfiI CCNNNNNNNGG 2 cut(s) 304, 348
AjnI CCWGG 2 cut(s) 92, 269
AluBI AGCT 2 cut(s) 155, 234
AluI AGCT 2 cut(s) 155, 234
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 1 cut(s) 306
ApaI GGGCCC 1 cut(s) 310
ApeKI GCWGC 4 cut(s) 155, 244, 333, 336
ApoI RAATTY 1 cut(s) 358
AspLEI GCGC 6 cut(s) 44, 149, 244, 276, 281, 341
AspS9I GGNCC 3 cut(s) 76, 306, 307
AsuC2I CCSGG 2 cut(s) 303, 304
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 76
BaeGI GKGCMC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 2 cut(s) 134, 310
BauI CACGAG 1 cut(s) 346
BbvI GCAGC 4 cut(s) 142, 231, 320, 348
BccI CCATC 1 cut(s) 350
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 2 cut(s) 94, 271
BciVI GTATCC 1 cut(s) 90
BcnI CCSGG 2 cut(s) 303, 304
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 4 cut(s) 156, 245, 322, 334
BfoI RGCGCY 2 cut(s) 282, 342
BfuI GTATCC 1 cut(s) 90
BisI GCNGC 9 cut(s) 105, 108, 156, 245, 277, 328, 331, 334, 337
BlsI GCNGC 9 cut(s) 106, 109, 157, 246, 278, 329, 332, 335, 338
Bme1390I CCNGG 4 cut(s) 94, 271, 303, 304
Bme18I GGWCC 1 cut(s) 76
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 3 cut(s) 76, 306, 307
BmiI GGNNCC 4 cut(s) 133, 280, 307, 308
BmrFI CCNGG 4 cut(s) 94, 271, 303, 304
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 253
Bpu10I CCTNAGC 1 cut(s) 151
BpuMI CCSGG 2 cut(s) 303, 304
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 302, 303, 328
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 348
Bse1I ACTGG 1 cut(s) 310
BseBI CCWGG 2 cut(s) 94, 271
BseDI CCNNGG 3 cut(s) 302, 303, 328
BseLI CCNNNNNNNGG 2 cut(s) 304, 348
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 2 cut(s) 17, 20
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 4 cut(s) 142, 231, 320, 348
BsgI GTGCAG 1 cut(s) 373
Bsh1236I CGCG 2 cut(s) 276, 330
BshFI GGCC 1 cut(s) 308
BshNI GGYRCC 1 cut(s) 278
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 1 cut(s) 303
BslI CCNNNNNNNGG 2 cut(s) 304, 348
BsnI GGCC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 302
Bsp120I GGGCCC 1 cut(s) 306
Bsp1286I GDGCHC 2 cut(s) 134, 310
Bsp143I GATC 2 cut(s) 36, 195
BspACI CCGC 7 cut(s) 56, 102, 105, 108, 276, 328, 330
BspANI GGCC 1 cut(s) 308
BspCNI CTCAG 1 cut(s) 203
BspFNI CGCG 2 cut(s) 276, 330
BspLI GGNNCC 4 cut(s) 133, 280, 307, 308
BspMAI CTGCAG 3 cut(s) 160, 249, 338
BspT107I GGYRCC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 302, 303, 328
BssMI GATC 2 cut(s) 36, 195
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 346
Bst2UI CCWGG 2 cut(s) 94, 271
Bst4CI ACNGT 3 cut(s) 17, 239, 259
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 124, 299
BstDEI CTNAG 3 cut(s) 151, 190, 211
BstDSI CCRYGG 1 cut(s) 328
BstFNI CGCG 2 cut(s) 276, 330
BstH2I RGCGCY 2 cut(s) 282, 342
BstHHI GCGC 6 cut(s) 44, 149, 244, 276, 281, 341
BstKTI GATC 2 cut(s) 39, 198
BstMBI GATC 2 cut(s) 36, 195
BstMWI GCNNNNNNNGC 5 cut(s) 146, 155, 273, 333, 336
BstNI CCWGG 2 cut(s) 94, 271
BstSCI CCNGG 4 cut(s) 92, 269, 301, 302
BstSFI CTRYAG 4 cut(s) 156, 245, 322, 334
BstSLI GKGCMC 1 cut(s) 310
BstUI CGCG 2 cut(s) 276, 330
BstV1I GCAGC 4 cut(s) 142, 231, 320, 348
BsuI GTATCC 1 cut(s) 90
BsuRI GGCC 1 cut(s) 308
BtgI CCRYGG 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 124, 299
CfoI GCGC 6 cut(s) 44, 149, 244, 276, 281, 341
Cfr13I GGNCC 3 cut(s) 76, 306, 307
Cfr42I CCGCGG 1 cut(s) 331
Cfr9I CCCGGG 1 cut(s) 302
CviAII CATG 1 cut(s) 184
DdeI CTNAG 3 cut(s) 151, 190, 211
DinI GGCGCC 1 cut(s) 280
DpnI GATC 2 cut(s) 38, 197
DpnII GATC 2 cut(s) 36, 195
DraIII CACNNNGTG 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 134, 310
Eco47I GGWCC 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 92, 269
EcoT38I GRGCYC 2 cut(s) 134, 310
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
FaeI CATG 1 cut(s) 187
FaiI YATR 2 cut(s) 137, 185
FatI CATG 1 cut(s) 183
FauI CCCGC 1 cut(s) 109
Fnu4HI GCNGC 9 cut(s) 105, 108, 156, 245, 277, 328, 331, 334, 337
FriOI GRGCYC 2 cut(s) 134, 310
Fsp4HI GCNGC 9 cut(s) 105, 108, 156, 245, 277, 328, 331, 334, 337
FspBI CTAG 1 cut(s) 6
GlaI GCGC 6 cut(s) 43, 148, 243, 275, 280, 340
GluI GCNGC 9 cut(s) 105, 108, 156, 245, 277, 328, 331, 334, 337
GsuI CTGGAG 1 cut(s) 253
HaeII RGCGCY 2 cut(s) 282, 342
HaeIII GGCC 1 cut(s) 308
HapII CCGG 1 cut(s) 303
HhaI GCGC 6 cut(s) 44, 149, 244, 276, 281, 341
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 6 cut(s) 42, 147, 242, 274, 279, 339
HinP1I GCGC 6 cut(s) 42, 147, 242, 274, 279, 339
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 303
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 2 cut(s) 34, 346
Hpy99I CGWCG 1 cut(s) 207
HpyCH4III ACNGT 3 cut(s) 17, 239, 259
HpyCH4V TGCA 6 cut(s) 13, 126, 158, 247, 336, 354
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 155, 273, 333, 336
HpyF3I CTNAG 3 cut(s) 151, 190, 211
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 6 cut(s) 42, 147, 242, 274, 279, 339
KasI GGCGCC 1 cut(s) 278
KspI CCGCGG 1 cut(s) 331
Kzo9I GATC 2 cut(s) 36, 195
LmnI GCTCC 2 cut(s) 137, 272
Lsp1109I GCAGC 4 cut(s) 142, 231, 320, 348
LweI GCATC 1 cut(s) 216
MaeI CTAG 1 cut(s) 6
MalI GATC 2 cut(s) 38, 197
MboI GATC 2 cut(s) 36, 195
MfeI CAATTG 1 cut(s) 292
MhlI GDGCHC 2 cut(s) 134, 310
MluCI AATT 2 cut(s) 292, 358
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 4 cut(s) 94, 271, 303, 304
MteI GCGCNGCGC 1 cut(s) 277
MunI CAATTG 1 cut(s) 292
MvaI CCWGG 2 cut(s) 94, 271
MvnI CGCG 2 cut(s) 276, 330
MwoI GCNNNNNNNGC 5 cut(s) 146, 155, 273, 333, 336
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 2 cut(s) 303, 304
NdeII GATC 2 cut(s) 36, 195
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 4 cut(s) 133, 280, 307, 308
PfoI TCCNGGA 1 cut(s) 92
PkrI GCNGC 9 cut(s) 106, 109, 157, 246, 278, 329, 332, 335, 338
PleI GAGTC 1 cut(s) 182
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 182
Psp6I CCWGG 2 cut(s) 92, 269
PspGI CCWGG 2 cut(s) 92, 269
PspN4I GGNNCC 4 cut(s) 133, 280, 307, 308
PspOMI GGGCCC 1 cut(s) 306
PspPI GGNCC 3 cut(s) 76, 306, 307
PstI CTGCAG 3 cut(s) 160, 249, 338
SacII CCGCGG 1 cut(s) 331
SatI GCNGC 9 cut(s) 105, 108, 156, 245, 277, 328, 331, 334, 337
Sau3AI GATC 2 cut(s) 36, 195
Sau96I GGNCC 3 cut(s) 76, 306, 307
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 4 cut(s) 94, 271, 303, 304
SduI GDGCHC 2 cut(s) 134, 310
SetI ASST 5 cut(s) 90, 157, 174, 236, 353
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 4 cut(s) 156, 245, 322, 334
SfoI GGCGCC 1 cut(s) 280
Sfr303I CCGCGG 1 cut(s) 331
SgrBI CCGCGG 1 cut(s) 331
SinI GGWCC 1 cut(s) 76
SmaI CCCGGG 1 cut(s) 304
Sse9I AATT 2 cut(s) 292, 358
SsiI CCGC 7 cut(s) 56, 102, 105, 108, 276, 328, 330
SspDI GGCGCC 1 cut(s) 278
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 4 cut(s) 92, 269, 301, 302
TaaI ACNGT 3 cut(s) 17, 239, 259
TaqI TCGA 1 cut(s) 370
TasI AATT 2 cut(s) 292, 358
TauI GCSGC 5 cut(s) 107, 110, 279, 330, 333
TseI GCWGC 4 cut(s) 155, 244, 333, 336
TspMI CCCGGG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 1 cut(s) 358
XmaI CCCGGG 1 cut(s) 302
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.