Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004612

Physical Location & Seq

Genomic Coordinates Reverse (-)

13663782 .. 13664162

Gene Structure 381 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1168800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 381 bp

ATGGGAAGCTCAAGCTCAAGAACTCCATCACTCTTTGGCTGCATCTTAATCTGTGCAATCCTGGCCGCCCATTTGGTAGCGATTGCTTCGGCCGTTGATTTCAGCGGCGGCCACCAGCTGGGCGAGTTGAGCTGGGGTTCGATTAGATCGGCGGCGGGGGGACTGATAGCTGACGAGGACGAGGAAGCGGAGATGCTGATGGACTCGGAGATCAACCGGCGCATCTTAGCCTGGAGGAGGAGGTACATTAGCTACGGTGCGCTGAGGAGGAACACTGTACCCTGCTCGCGACGTGGCGCGTCCTACTACAACTGCCGACCCGGGGCTCAGGCAAATCCCTACCGTCGCGGCTGTAGCGCCATTACTCGCTGCAGGCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

127

Amino Acids

13.77

Weight (kDa)

9.54

Isoelectric Point (pI)

63.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	64 - 126	7.9e-31	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004612)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15800
fragaria_vesca	FvH4_4g18001 FvH4_6g06520
malus_domestica	MD03G1171400.v1.1 MD04G1201100.v1.1 MD12G1213600.v1.1 MD13G1168800.v1.1 MD16G1169200.v1.1
prunus_persica	Prupe.1G137000_v2.0.a1 Prupe.6G321200_v2.0.a1
pyrus_communis	pycom12g19950 pycom13g14440 pycom16g14230 pycom16g14250
rosa_chinensis	RchiOBHm_Chr3g0455671 RchiOBHm_Chr4g0422211
rosa_multiflora	Rmu_sc0002883.1_g000009 Rmu_sc0014362.1_g000002 Rmu_sc0026117.1_g000002
rosa_roxburghii	Rroxscaffold_5G00364330 Rroxscaffold_6G00423610 Rroxscaffold_6G00424110
rosa_rugosa	Rorug03G0009000 Rorug04G0178800
rosa_samantha	Rh3AG068700 Rh3BG071200 Rh3CG069500 Rh3DG071100 Rh4CG252800
rosa_wichuraiana	Rw3G005510 Rw4G020570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	118
AccII	CGCG	3 cut(s)	289, 299, 348
AciI	CCGC	7 cut(s)	66, 105, 108, 152, 155, 188, 348
AcoI	YGGCCR	3 cut(s)	63, 90, 109
AfaI	GTAC	2 cut(s)	245, 279
AfiI	CCNNNNNNNGG	3 cut(s)	118, 237, 322
AjiI	CACGTC	1 cut(s)	293
AjnI	CCWGG	2 cut(s)	60, 230
AluBI	AGCT	6 cut(s)	9, 15, 118, 132, 170, 252
AluI	AGCT	6 cut(s)	9, 15, 118, 132, 170, 252
Ama87I	CYCGRG	1 cut(s)	320
AoxI	GGCC	3 cut(s)	63, 90, 109
ApeKI	GCWGC	2 cut(s)	39, 369
AspLEI	GCGC	5 cut(s)	222, 262, 299, 359, 378
AsuC2I	CCSGG	2 cut(s)	321, 322
AvaI	CYCGRG	1 cut(s)	320
BanII	GRGCYC	1 cut(s)	328
BbvCI	CCTCAGC	1 cut(s)	263
BbvI	GCAGC	2 cut(s)	26, 356
BccI	CCATC	2 cut(s)	34, 193
BceAI	ACGGC	1 cut(s)	77
BciT130I	CCWGG	2 cut(s)	62, 232
BcnI	CCSGG	2 cut(s)	321, 322
BfmI	CTRYAG	2 cut(s)	352, 370
BfoI	RGCGCY	2 cut(s)	360, 379
BisI	GCNGC	7 cut(s)	40, 66, 106, 109, 153, 349, 370
BlsI	GCNGC	7 cut(s)	41, 67, 107, 110, 154, 350, 371
Bme1390I	CCNGG	4 cut(s)	62, 232, 321, 322
BmeT110I	CYCGRG	1 cut(s)	320
BmgBI	CACGTC	1 cut(s)	293
BmrFI	CCNGG	4 cut(s)	62, 232, 321, 322
BmsI	GCATC	3 cut(s)	51, 183, 231
BpmI	CTGGAG	1 cut(s)	253
Bpu10I	CCTNAGC	2 cut(s)	263, 327
BpuMI	CCSGG	2 cut(s)	321, 322
BsaJI	CCNNGG	2 cut(s)	320, 321
Bsc4I	CCNNNNNNNGG	3 cut(s)	118, 237, 322
Bse118I	RCCGGY	1 cut(s)	216
BseBI	CCWGG	2 cut(s)	62, 232
BseDI	CCNNGG	2 cut(s)	320, 321
BseLI	CCNNNNNNNGG	3 cut(s)	118, 237, 322
BseMII	CTCAG	2 cut(s)	254, 341
BseRI	GAGGAG	3 cut(s)	250, 253, 280
BseX3I	CGGCCG	1 cut(s)	90
BseXI	GCAGC	2 cut(s)	26, 356
BseYI	CCCAGC	2 cut(s)	118, 132
Bsh1236I	CGCG	3 cut(s)	289, 299, 348
Bsh1285I	CGRYCG	1 cut(s)	93
BshFI	GGCC	3 cut(s)	65, 92, 111
BsiEI	CGRYCG	1 cut(s)	93
BsiHKCI	CYCGRG	1 cut(s)	320
BsiSI	CCGG	2 cut(s)	217, 321
BslFI	GGGAC	1 cut(s)	174
BslI	CCNNNNNNNGG	3 cut(s)	118, 237, 322
BsmFI	GGGAC	1 cut(s)	174
BsnI	GGCC	3 cut(s)	65, 92, 111
BsoBI	CYCGRG	1 cut(s)	320
Bsp1286I	GDGCHC	1 cut(s)	328
Bsp143I	GATC	2 cut(s)	146, 210
Bsp68I	TCGCGA	1 cut(s)	289
BspACI	CCGC	7 cut(s)	66, 105, 108, 152, 155, 188, 348
BspANI	GGCC	3 cut(s)	65, 92, 111
BspCNI	CTCAG	2 cut(s)	255, 340
BspFNI	CGCG	3 cut(s)	289, 299, 348
BspMAI	CTGCAG	1 cut(s)	374
BsrFI	RCCGGY	1 cut(s)	216
BssAI	RCCGGY	1 cut(s)	216
BssECI	CCNNGG	2 cut(s)	320, 321
BssMI	GATC	2 cut(s)	146, 210
Bst2UI	CCWGG	2 cut(s)	62, 232
Bst4CI	ACNGT	3 cut(s)	257, 277, 344
BstC8I	GCNNGC	2 cut(s)	287, 374
BstDEI	CTNAG	3 cut(s)	226, 263, 327
BstENI	CCTNNNNNAGG	1 cut(s)	235
BstFNI	CGCG	3 cut(s)	289, 299, 348
BstH2I	RGCGCY	2 cut(s)	360, 379
BstHHI	GCGC	5 cut(s)	222, 262, 299, 359, 378
BstKTI	GATC	2 cut(s)	149, 213
BstMBI	GATC	2 cut(s)	146, 210
BstMCI	CGRYCG	1 cut(s)	93
BstMWI	GCNNNNNNNGC	4 cut(s)	62, 129, 354, 375
BstNI	CCWGG	2 cut(s)	62, 232
BstSCI	CCNGG	4 cut(s)	60, 230, 319, 320
BstSFI	CTRYAG	2 cut(s)	352, 370
BstUI	CGCG	3 cut(s)	289, 299, 348
BstV1I	GCAGC	2 cut(s)	26, 356
BstZI	CGGCCG	1 cut(s)	90
BsuRI	GGCC	3 cut(s)	65, 92, 111
BtrI	CACGTC	1 cut(s)	293
BtsIMutI	CAGTG	1 cut(s)	273
BtuMI	TCGCGA	1 cut(s)	289
Cac8I	GCNNGC	2 cut(s)	287, 374
CfoI	GCGC	5 cut(s)	222, 262, 299, 359, 378
Cfr10I	RCCGGY	1 cut(s)	216
Cfr9I	CCCGGG	1 cut(s)	320
CseI	GACGC	1 cut(s)	288
Csp6I	GTAC	2 cut(s)	244, 278
CviQI	GTAC	2 cut(s)	244, 278
DdeI	CTNAG	3 cut(s)	226, 263, 327
DpnI	GATC	2 cut(s)	148, 212
DpnII	GATC	2 cut(s)	146, 210
EaeI	YGGCCR	3 cut(s)	63, 90, 109
EagI	CGGCCG	1 cut(s)	90
EclXI	CGGCCG	1 cut(s)	90
Eco24I	GRGCYC	1 cut(s)	328
Eco52I	CGGCCG	1 cut(s)	90
Eco88I	CYCGRG	1 cut(s)	320
EcoNI	CCTNNNNNAGG	1 cut(s)	235
EcoRII	CCWGG	2 cut(s)	60, 230
EcoT38I	GRGCYC	1 cut(s)	328
FaqI	GGGAC	1 cut(s)	174
FauI	CCCGC	1 cut(s)	148
Fnu4HI	GCNGC	7 cut(s)	40, 66, 106, 109, 153, 349, 370
FriOI	GRGCYC	1 cut(s)	328
Fsp4HI	GCNGC	7 cut(s)	40, 66, 106, 109, 153, 349, 370
GlaI	GCGC	5 cut(s)	221, 261, 298, 358, 377
GluI	GCNGC	7 cut(s)	40, 66, 106, 109, 153, 349, 370
GsaI	CCCAGC	2 cut(s)	122, 136
GsuI	CTGGAG	1 cut(s)	253
HaeII	RGCGCY	2 cut(s)	360, 379
HaeIII	GGCC	3 cut(s)	65, 92, 111
HapII	CCGG	2 cut(s)	217, 321
HgaI	GACGC	1 cut(s)	288
HhaI	GCGC	5 cut(s)	222, 262, 299, 359, 378
Hin6I	GCGC	5 cut(s)	220, 260, 297, 357, 376
HinP1I	GCGC	5 cut(s)	220, 260, 297, 357, 376
HinfI	GANTC	1 cut(s)	203
HpaII	CCGG	2 cut(s)	217, 321
Hpy188I	TCNGA	1 cut(s)	208
Hpy188III	TCNNGA	2 cut(s)	18, 288
Hpy99I	CGWCG	2 cut(s)	294, 348
HpyCH4III	ACNGT	3 cut(s)	257, 277, 344
HpyCH4IV	ACGT	1 cut(s)	292
HpyCH4V	TGCA	3 cut(s)	42, 56, 372
HpyF10VI	GCNNNNNNNGC	4 cut(s)	62, 129, 354, 375
HpyF3I	CTNAG	3 cut(s)	226, 263, 327
HpySE526I	ACGT	1 cut(s)	292
HspAI	GCGC	5 cut(s)	220, 260, 297, 357, 376
Kzo9I	GATC	2 cut(s)	146, 210
Lsp1109I	GCAGC	2 cut(s)	26, 356
LweI	GCATC	3 cut(s)	51, 183, 231
MaeII	ACGT	1 cut(s)	292
MalI	GATC	2 cut(s)	148, 212
MboI	GATC	2 cut(s)	146, 210
MhlI	GDGCHC	1 cut(s)	328
MlyI	GAGTC	1 cut(s)	197
MnlI	CCTC	7 cut(s)	169, 175, 228, 231, 234, 258, 261
MseI	TTAA	1 cut(s)	47
MspA1I	CMGCKG	2 cut(s)	105, 118
MspI	CCGG	2 cut(s)	217, 321
MspR9I	CCNGG	4 cut(s)	62, 232, 321, 322
MvaI	CCWGG	2 cut(s)	62, 232
MvnI	CGCG	3 cut(s)	289, 299, 348
MwoI	GCNNNNNNNGC	4 cut(s)	62, 129, 354, 375
NciI	CCSGG	2 cut(s)	321, 322
NdeII	GATC	2 cut(s)	146, 210
NruI	TCGCGA	1 cut(s)	289
PflMI	CCANNNNNTGG	1 cut(s)	118
PkrI	GCNGC	7 cut(s)	41, 67, 107, 110, 154, 350, 371
PleI	GAGTC	1 cut(s)	197
PpsI	GAGTC	1 cut(s)	197
Psp6I	CCWGG	2 cut(s)	60, 230
PspFI	CCCAGC	2 cut(s)	118, 132
PspGI	CCWGG	2 cut(s)	60, 230
PstI	CTGCAG	1 cut(s)	374
PvuII	CAGCTG	1 cut(s)	118
RruI	TCGCGA	1 cut(s)	289
RsaI	GTAC	2 cut(s)	245, 279
RsaNI	GTAC	2 cut(s)	244, 278
SaqAI	TTAA	1 cut(s)	47
SatI	GCNGC	7 cut(s)	40, 66, 106, 109, 153, 349, 370
Sau3AI	GATC	2 cut(s)	146, 210
SchI	GAGTC	1 cut(s)	197
ScrFI	CCNGG	4 cut(s)	62, 232, 321, 322
SduI	GDGCHC	1 cut(s)	328
SetI	ASST	8 cut(s)	11, 17, 120, 134, 172, 245, 254, 295
SfaNI	GCATC	3 cut(s)	51, 183, 231
SfcI	CTRYAG	2 cut(s)	352, 370
SmaI	CCCGGG	1 cut(s)	322
SmlI	CTYRAG	2 cut(s)	10, 16
SmoI	CTYRAG	2 cut(s)	10, 16
SsiI	CCGC	7 cut(s)	66, 105, 108, 152, 155, 188, 348
StyD4I	CCNGG	4 cut(s)	60, 230, 319, 320
TaaI	ACNGT	3 cut(s)	257, 277, 344
TaiI	ACGT	1 cut(s)	295
TaqI	TCGA	1 cut(s)	140
TauI	GCSGC	5 cut(s)	68, 108, 111, 155, 351
Tru1I	TTAA	1 cut(s)	47
Tru9I	TTAA	1 cut(s)	47
TscAI	CASTG	1 cut(s)	280
TseI	GCWGC	2 cut(s)	39, 369
TspMI	CCCGGG	1 cut(s)	320
TspRI	CASTG	1 cut(s)	280
Van91I	CCANNNNNTGG	1 cut(s)	118
XagI	CCTNNNNNAGG	1 cut(s)	235
XmaI	CCCGGG	1 cut(s)	320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.